pycom12g09230

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010662
Physical Location & Seq
Reverse (-)
11136737 .. 11140101
3365 bp
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UTR
Exon/CDS
Intron
pycom12g09230.1

Sequence Viewer

Length: 558 bp
ATGGCACAGAATAAACCCAGTAGGCTCTACCAAGTTTGGAAAGGAAGCAATAAGTTTTTCTGTGGCGGGAGATTAATTTTTGGTCCTGATGTGGCATCTCTTCTTCTGTCTACACTCCTTGTAGCCGGCCCAGCAGTTGCCTTCTGTTTGAAGGTTTATTTTAAAATAAAAAATGGGAATCACAAGAATGACAATTTATGGCTTCCTGTATTAGCTATTGGCTCACTCCTTACAATTTTGGATTTAACCTTTCTCTTCTTGACTTCTGGGAGGGATCCTGGAATAATCCCTCGAAATTCAAGGCCTCCAGAATCAGATGAAGCATTTGATCTAGCTACCCCATCCATGGAGTGGGTTAATGAAAGAACTCCTCATTTGAAACTACCTCGAACAAAAGATGTCGTTGTAAATGGTCACACAGTAAAAGTGAAGTATTGCGATACTTGTTTTCTTTATCGCCCTCCCCGCGTATCTCACTGTTCTATCTGCAACAATTGTGTTCAGAGATTCGATCATCACTGTCCATGGGTTGGTCAGTGCATTGGAATTTCCCTATAA

Protein Analysis

186

Amino Acids

20.76

Weight (kDa)

9.14

Isoelectric Point (pI)

22.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 143 - 182 6.9e-17 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010662)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56930 AT3G56930
fragaria_vesca FvH4_1g28800 FvH4_1g28800 FvH4_1g28800
malus_domestica MD00G1107600.v1.1 MD14G1100600.v1.1
prunus_persica Prupe.7G022600_v2.0.a1
pyrus_communis pycom12g09230 pycom14g08900
rosa_chinensis RchiOBHm_Chr3g0493221
rosa_laevigata RLG00000022721
rosa_multiflora Rmu_sc0003406.1_g000006
rosa_roxburghii Rroxscaffold_6G00391740
rosa_rugosa Rorug03G0259900
rosa_samantha Rh3AG308800 Rh3BG344800 Rh3CG342000 Rh3DG344100
rosa_wichuraiana Rw3G027150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 351, 530
AccI GTMKAC 1 cut(s) 110
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 66, 466
AclWI GGATC 2 cut(s) 269, 282
AcsI RAATTY 2 cut(s) 295, 546
AfiI CCNNNNNNNGG 3 cut(s) 346, 351, 530
AgsI TTSAA 3 cut(s) 151, 300, 379
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 2 cut(s) 215, 335
AluI AGCT 2 cut(s) 215, 335
AlwI GGATC 2 cut(s) 269, 282
AoxI GGCC 2 cut(s) 127, 302
ApoI RAATTY 2 cut(s) 295, 546
AseI ATTAAT 1 cut(s) 74
AspS9I GGNCC 2 cut(s) 83, 128
AvaII GGWCC 1 cut(s) 83
BamHI GGATCC 1 cut(s) 274
BccI CCATC 1 cut(s) 349
BciT130I CCWGG 1 cut(s) 279
BfaI CTAG 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 83, 128
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 279
BmrI ACTGGG 1 cut(s) 12
BmsI GCATC 1 cut(s) 104
BmuI ACTGGG 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 345, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 351, 530
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 345, 524
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 3 cut(s) 346, 351, 530
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 1 cut(s) 360
BseYI CCCAGC 1 cut(s) 130
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 2 cut(s) 129, 304
BsiSI CCGG 1 cut(s) 126
BslI CCNNNNNNNGG 3 cut(s) 346, 351, 530
BsnI GGCC 2 cut(s) 129, 304
Bsp143I GATC 3 cut(s) 274, 328, 511
Bsp19I CCATGG 2 cut(s) 345, 524
BspACI CCGC 2 cut(s) 66, 466
BspANI GGCC 2 cut(s) 129, 304
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 1 cut(s) 276
BspPI GGATC 2 cut(s) 269, 282
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 345, 524
BssMI GATC 3 cut(s) 274, 328, 511
BssT1I CCWWGG 2 cut(s) 345, 524
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 421, 479, 521
Bst6I CTCTTC 2 cut(s) 105, 260
BstC8I GCNNGC 1 cut(s) 127
BstDSI CCRYGG 2 cut(s) 345, 524
BstF5I GGATG 1 cut(s) 341
BstFNI CGCG 1 cut(s) 468
BstKTI GATC 3 cut(s) 277, 331, 514
BstMBI GATC 3 cut(s) 274, 328, 511
BstMWI GCNNNNNNNGC 2 cut(s) 131, 465
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstUI CGCG 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 2 cut(s) 129, 304
BtgI CCRYGG 2 cut(s) 345, 524
BtsCI GGATG 1 cut(s) 341
BtsIMutI CAGTG 3 cut(s) 475, 517, 542
Cac8I GCNNGC 1 cut(s) 127
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 83, 128
CviAII CATG 2 cut(s) 346, 525
CviJI RGCY 8 cut(s) 25, 125, 129, 202, 215, 222, 304, 335
CviKI_1 RGCY 8 cut(s) 25, 125, 129, 202, 215, 222, 304, 335
DpnI GATC 3 cut(s) 276, 330, 513
DpnII GATC 3 cut(s) 274, 328, 511
DraI TTTAAA 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 105, 260
EarI CTCTTC 2 cut(s) 105, 260
Eco130I CCWWGG 2 cut(s) 345, 524
Eco147I AGGCCT 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 345, 524
ErhI CCWWGG 2 cut(s) 345, 524
FaeI CATG 2 cut(s) 349, 528
FaiI YATR 4 cut(s) 199, 347, 526, 556
FatI CATG 2 cut(s) 345, 524
FauI CCCGC 2 cut(s) 59, 473
FblI GTMKAC 1 cut(s) 110
FokI GGATG 1 cut(s) 328
FspBI CTAG 1 cut(s) 332
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 2 cut(s) 129, 304
HapII CCGG 1 cut(s) 126
Hin1II CATG 2 cut(s) 349, 528
HinfI GANTC 3 cut(s) 178, 311, 507
HpaII CCGG 1 cut(s) 126
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 2 cut(s) 316, 504
Hpy188III TCNNGA 3 cut(s) 86, 259, 308
Hpy8I GTNNAC 1 cut(s) 111
HpyAV CCTTC 2 cut(s) 145, 151
HpyCH4III ACNGT 3 cut(s) 421, 479, 521
HpyCH4V TGCA 2 cut(s) 489, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 465
Hsp92II CATG 2 cut(s) 349, 528
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 274, 328, 511
LpnPI CCDG 9 cut(s) 31, 99, 139, 144, 219, 252, 264, 291, 321
LweI GCATC 1 cut(s) 104
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 413
MalI GATC 3 cut(s) 276, 330, 513
MboI GATC 3 cut(s) 274, 328, 511
MboII GAAGA 3 cut(s) 92, 95, 247
MfeI CAATTG 1 cut(s) 493
MflI RGATCY 1 cut(s) 274
MluCI AATT 6 cut(s) 75, 193, 234, 295, 493, 546
MnlI CCTC 6 cut(s) 264, 300, 315, 381, 396, 471
MroNI GCCGGC 1 cut(s) 125
MseI TTAA 4 cut(s) 74, 162, 245, 357
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 279
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 131, 465
NaeI GCCGGC 1 cut(s) 127
NcoI CCATGG 2 cut(s) 345, 524
NdeII GATC 3 cut(s) 274, 328, 511
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 2 cut(s) 349, 528
NlaIV GGNNCC 1 cut(s) 276
NmuCI GTSAC 1 cut(s) 413
PceI AGGCCT 1 cut(s) 304
PdiI GCCGGC 1 cut(s) 127
PfeI GAWTC 3 cut(s) 178, 311, 507
PflMI CCANNNNNTGG 2 cut(s) 351, 530
PfoI TCCNGGA 1 cut(s) 277
PshBI ATTAAT 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 2 cut(s) 83, 128
PsuI RGATCY 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 4 cut(s) 74, 162, 245, 357
Sau3AI GATC 3 cut(s) 274, 328, 511
Sau96I GGNCC 2 cut(s) 83, 128
ScrFI CCNGG 1 cut(s) 279
SetI ASST 5 cut(s) 156, 217, 251, 337, 388
SfaNI GCATC 1 cut(s) 104
SinI GGWCC 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 6 cut(s) 75, 193, 234, 295, 493, 546
SseBI AGGCCT 1 cut(s) 304
SsiI CCGC 2 cut(s) 66, 466
SspMI CTAG 1 cut(s) 332
StuI AGGCCT 1 cut(s) 304
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 2 cut(s) 345, 524
TaaI ACNGT 3 cut(s) 421, 479, 521
TaqI TCGA 3 cut(s) 292, 388, 510
TasI AATT 6 cut(s) 75, 193, 234, 295, 493, 546
TfiI GAWTC 3 cut(s) 178, 311, 507
Tru1I TTAA 4 cut(s) 74, 162, 245, 357
Tru9I TTAA 4 cut(s) 74, 162, 245, 357
TscAI CASTG 3 cut(s) 482, 524, 542
TseFI GTSAC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 2 cut(s) 333, 375
TspRI CASTG 3 cut(s) 482, 524, 542
Van91I CCANNNNNTGG 2 cut(s) 351, 530
VpaK11BI GGWCC 1 cut(s) 83
VspI ATTAAT 1 cut(s) 74
XapI RAATTY 2 cut(s) 295, 546
XcmI CCANNNNNNNNNTGG 1 cut(s) 348
XmiI GTMKAC 1 cut(s) 110
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.