pycom12g10250

TPL-binding domain in jasmonate signalling

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
Physical Location & Seq
Forward (+)
12236877 .. 12240970
4094 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g10250.11

Sequence Viewer

Length: 1509 bp
ATGGAAGATAACACCAAATATCTATCTTCAAAATCAATTCTAATCTCAAGGTCCCCTAGTACTTCTTCAGTGTATTTTTCTTCGCAGAAGAAAAGTCAGTGGAGGATTACAACAAAAGATCAGCGGCTGCATAAGTTGGTTTTCGAGGAAGGTGGATTGCCTGACGGAACAGAAGTAGCATATTATGCACGCGGACAGAAATTGCTAGTTGGTTACAAGAAAGGATTTGGAATATTTTGTTGTTGCTGCAACAGTGAGGTCAGCCCGTCACAGTTTGAGGCCCATGCAGGTTGGGCCACACGCAGGAAACCCTATGCATACATTTACACATCTAATGGAGTGTCTCTCCACGAGTTGGCTATATCTTTGTCAAGAGGTCGGAAATATGCAGCCAAGGATAATGATGACTTGTGCATCATTTGTGCCGATGGTGGGAACCTTGTGCTTTGTGATGGATGCCCTAGGGCCTTCCACAGAGACTGTGCTTCTCTACCAAGTGTTCCTCGTGGTGACTGGTACTGTAAGTTTTGTCAAAATATGTTTCAAAGAGAAAAGTTTGTGGAACATAATGAAAATGCTGTCGCTGCTGGAAGGATTGATGGAATTGATCGATCTATAGAACAGATAACCCAAAGATGCATTCGTATTGTCAAAGACATAGAAGCTGAACTAACTGGATGCGTTTTATGTAGAGGTTATGATTTTAGCAAATCAGGATTTGGTCCACGAACTATTATACTTTGTGATCAGTGTGAGAAGGAATATCATGTTGGCTGTTTGAAGAAACATAAAATGGCAAATTTAAAGGAATTGCCAAAAGGGAAGTGGTTTTGTTGCGCAGACTGCAGTAGAATTCATTCTATTTTGCAAAGGTTGCTGACTAGAGGAGCTGAGAAGCTTCCTGACTCTCATTTGGATGTTATAAAGAAGAAGATGGAGGCAAACGGTTTGGAGACTGTCAGTGGGTTTGATGTGAGATGGAGACTCATTAGTGGCAAAATTGCTTCTCAGGAATGTCGTTTTTTACTTTCAAAGGCTGTTGCTATCTTCCATGATTGTTTTGATCCTATAGTTGATGCAGAATCTGGGCGAGACCTTATTCCCGCCATGGTTTATGGAAGGAATGTTAGGAGCCAAGAATTTGGGAATATGTACTGTGCAATACTGATGGTCAACTCGACTGTGGTATCAGCTGGAATTATACGTGTTTTTGGTCATGAAGTTGCTGAACTTCCTTTGGTTGCCACTAGTAATGGTAACCATGGCAAGGGCTACTTCCAACTATTGTTCTCCTGCATTGAAAAGCTGCTAGCTTTCTTGAATGTGAAAAGTATTGTGCTGCCGGCTGCGGAAGAAGCAGAATCCATATGGACCGATAAATTTGGTTTTACAAAAATGAAGCCGGAGCAGCTCACCACCTACCGAAGAACCTGCTACCAAATGGTGACATTCAAAGGAACGTCCATGCTACATAAAAGGGTTCCCGAGTGCCGGGTTGTAAGCACATGA

Protein Analysis

503

Amino Acids

56.45

Weight (kDa)

8.83

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 923
Acc16I TGCGCA 1 cut(s) 838
Acc36I ACCTGC 2 cut(s) 278, 1439
AccB7I CCANNNNNTGG 1 cut(s) 355
AccII CGCG 1 cut(s) 192
AciI CCGC 4 cut(s) 124, 192, 1104, 1349
AclWI GGATC 1 cut(s) 1058
AcsI RAATTY 4 cut(s) 799, 852, 1139, 1379
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 3 cut(s) 61, 518, 1154
AfiI CCNNNNNNNGG 5 cut(s) 303, 355, 432, 1267, 1491
AflIII ACRYGT 1 cut(s) 1204
AgsI TTSAA 7 cut(s) 30, 545, 781, 1032, 1301, 1321, 1453
AhlI ACTAGT 1 cut(s) 1247
AjuI GAANNNNNNNTTGG 4 cut(s) 624, 656, 753, 785
AluBI AGCT 7 cut(s) 665, 890, 898, 1193, 1306, 1313, 1411
AluI AGCT 7 cut(s) 665, 890, 898, 1193, 1306, 1313, 1411
Alw26I GTCTC 5 cut(s) 348, 471, 947, 976, 1086
AlwI GGATC 1 cut(s) 1058
AlwNI CAGNNNCTG 3 cut(s) 127, 480, 1085
Ama87I CYCGRG 1 cut(s) 1484
AoxI GGCC 3 cut(s) 279, 294, 465
ApeKI GCWGC 8 cut(s) 127, 246, 389, 584, 1306, 1339, 1346, 1408
ApoI RAATTY 4 cut(s) 799, 852, 1139, 1379
Asp700I GAANNNNTTC 1 cut(s) 856
AspA2I CCTAGG 1 cut(s) 461
AspLEI GCGC 1 cut(s) 839
AspS9I GGNCC 6 cut(s) 51, 280, 294, 465, 722, 1371
AsuC2I CCSGG 1 cut(s) 1493
AsuHPI GGTGA 3 cut(s) 521, 1405, 1456
AsuNHI GCTAGC 1 cut(s) 1309
AvaI CYCGRG 1 cut(s) 1484
AvaII GGWCC 3 cut(s) 51, 722, 1371
AvrII CCTAGG 1 cut(s) 461
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BauI CACGAG 2 cut(s) 350, 504
BbvI GCAGC 8 cut(s) 114, 233, 401, 571, 1293, 1326, 1333, 1420
BccI CCATC 6 cut(s) 422, 446, 593, 928, 972, 1162
BcgI CGANNNNNNTGC 2 cut(s) 1413, 1447
BclI TGATCA 1 cut(s) 745
BcnI CCSGG 1 cut(s) 1493
BcoDI GTCTC 5 cut(s) 348, 471, 947, 976, 1086
BcuI ACTAGT 1 cut(s) 1247
BfaI CTAG 6 cut(s) 57, 206, 462, 882, 1248, 1310
BfmI CTRYAG 3 cut(s) 615, 844, 1068
BfuAI ACCTGC 2 cut(s) 278, 1439
BisI GCNGC 9 cut(s) 125, 128, 247, 390, 585, 1307, 1340, 1347, 1409
BlnI CCTAGG 1 cut(s) 461
BlsI GCNGC 9 cut(s) 126, 129, 248, 391, 586, 1308, 1341, 1348, 1410
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 1493
Bme18I GGWCC 3 cut(s) 51, 722, 1371
BmeT110I CYCGRG 1 cut(s) 1484
BmgT120I GGNCC 6 cut(s) 51, 280, 294, 465, 722, 1371
BmiI GGNNCC 4 cut(s) 53, 437, 1133, 1482
BmrFI CCNGG 1 cut(s) 1493
BmsI GCATC 5 cut(s) 423, 446, 626, 668, 1066
BmtI GCTAGC 1 cut(s) 1313
BplI GAGNNNNNCTC 2 cut(s) 330, 362
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 1493
Bsa29I ATCGAT 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 1205
BsaI GGTCTC 1 cut(s) 1086
BsaJI CCNNGG 4 cut(s) 393, 461, 1107, 1261
Bsc4I CCNNNNNNNGG 5 cut(s) 303, 355, 432, 1267, 1491
Bse118I RCCGGY 1 cut(s) 1342
Bse1I ACTGG 2 cut(s) 518, 679
BseCI ATCGAT 1 cut(s) 610
BseDI CCNNGG 4 cut(s) 393, 461, 1107, 1261
BseGI GGATG 3 cut(s) 461, 683, 922
BseLI CCNNNNNNNGG 5 cut(s) 303, 355, 432, 1267, 1491
BseMII CTCAG 2 cut(s) 882, 1022
BseNI ACTGG 2 cut(s) 518, 679
BseRI GAGGAG 1 cut(s) 900
BseXI GCAGC 8 cut(s) 114, 233, 401, 571, 1293, 1326, 1333, 1420
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 3 cut(s) 281, 296, 467
BshVI ATCGAT 1 cut(s) 610
BsiHKCI CYCGRG 1 cut(s) 1484
BsiSI CCGG 3 cut(s) 1343, 1403, 1492
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 5 cut(s) 303, 355, 432, 1267, 1491
BsmAI GTCTC 5 cut(s) 348, 471, 947, 976, 1086
BsmFI GGGAC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 3 cut(s) 281, 296, 467
Bso31I GGTCTC 1 cut(s) 1086
BsoBI CYCGRG 1 cut(s) 1484
Bsp143I GATC 5 cut(s) 118, 607, 611, 745, 1063
Bsp19I CCATGG 2 cut(s) 1107, 1261
BspACI CCGC 4 cut(s) 124, 192, 1104, 1349
BspANI GGCC 3 cut(s) 281, 296, 467
BspCNI CTCAG 2 cut(s) 883, 1021
BspDI ATCGAT 1 cut(s) 610
BspFNI CGCG 1 cut(s) 192
BspHI TCATGA 1 cut(s) 1216
BspLI GGNNCC 4 cut(s) 53, 437, 1133, 1482
BspMAI CTGCAG 1 cut(s) 848
BspMI ACCTGC 2 cut(s) 278, 1439
BspOI GCTAGC 1 cut(s) 1313
BspPI GGATC 1 cut(s) 1058
BspTNI GGTCTC 1 cut(s) 1086
BsrFI RCCGGY 1 cut(s) 1342
BsrI ACTGG 2 cut(s) 518, 679
BssAI RCCGGY 1 cut(s) 1342
BssECI CCNNGG 4 cut(s) 393, 461, 1107, 1261
BssMI GATC 5 cut(s) 118, 607, 611, 745, 1063
BssSI CACGAG 2 cut(s) 350, 504
BssT1I CCWWGG 4 cut(s) 393, 461, 1107, 1261
Bst2BI CACGAG 2 cut(s) 350, 504
Bst4CI ACNGT 8 cut(s) 254, 273, 482, 521, 947, 958, 1157, 1183
BstAPI GCANNNNNTGC 2 cut(s) 185, 874
BstBAI YACGTR 1 cut(s) 1205
BstC8I GCNNGC 3 cut(s) 190, 1311, 1344
BstDEI CTNAG 2 cut(s) 891, 1008
BstDSI CCRYGG 2 cut(s) 1107, 1261
BstEII GGTNACC 1 cut(s) 1256
BstF5I GGATG 3 cut(s) 461, 683, 922
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 839
BstKTI GATC 5 cut(s) 121, 610, 614, 748, 1066
BstMAI GTCTC 5 cut(s) 348, 471, 947, 976, 1086
BstMBI GATC 5 cut(s) 118, 607, 611, 745, 1063
BstMWI GCNNNNNNNGC 7 cut(s) 185, 293, 584, 843, 874, 1355, 1408
BstPI GGTNACC 1 cut(s) 1256
BstSCI CCNGG 1 cut(s) 1491
BstSFI CTRYAG 3 cut(s) 615, 844, 1068
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 8 cut(s) 114, 233, 401, 571, 1293, 1326, 1333, 1420
BstXI CCANNNNNNTGG 1 cut(s) 1142
Bsu15I ATCGAT 1 cut(s) 610
BsuRI GGCC 3 cut(s) 281, 296, 467
BsuTUI ATCGAT 1 cut(s) 610
BtgI CCRYGG 2 cut(s) 1107, 1261
BtsCI GGATG 3 cut(s) 461, 683, 922
BtsIMutI CAGTG 5 cut(s) 75, 104, 259, 755, 967
BveI ACCTGC 2 cut(s) 278, 1439
Cac8I GCNNGC 3 cut(s) 190, 1311, 1344
CaiI CAGNNNCTG 3 cut(s) 127, 480, 1085
CciI TCATGA 1 cut(s) 1216
CfoI GCGC 1 cut(s) 839
Cfr10I RCCGGY 1 cut(s) 1342
Cfr13I GGNCC 6 cut(s) 51, 280, 294, 465, 722, 1371
ClaI ATCGAT 1 cut(s) 610
Csp6I GTAC 3 cut(s) 60, 517, 1153
CviAII CATG 8 cut(s) 284, 767, 1052, 1108, 1217, 1262, 1465, 1506
CviQI GTAC 3 cut(s) 60, 517, 1153
DdeI CTNAG 2 cut(s) 891, 1008
DpnI GATC 5 cut(s) 120, 609, 613, 747, 1065
DpnII GATC 5 cut(s) 118, 607, 611, 745, 1063
DraI TTTAAA 1 cut(s) 804
Eco130I CCWWGG 4 cut(s) 393, 461, 1107, 1261
Eco31I GGTCTC 1 cut(s) 1086
Eco47I GGWCC 3 cut(s) 51, 722, 1371
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 1484
Eco91I GGTNACC 1 cut(s) 1256
EcoO109I RGGNCCY 2 cut(s) 51, 465
EcoO65I GGTNACC 1 cut(s) 1256
EcoRI GAATTC 1 cut(s) 852
EcoT14I CCWWGG 4 cut(s) 393, 461, 1107, 1261
EcoT22I ATGCAT 2 cut(s) 319, 641
ErhI CCWWGG 4 cut(s) 393, 461, 1107, 1261
FaeI CATG 8 cut(s) 287, 770, 1055, 1111, 1220, 1265, 1468, 1509
FalI AAGNNNNNCTT 2 cut(s) 1259, 1291
FaqI GGGAC 1 cut(s) 37
FatI CATG 8 cut(s) 283, 766, 1051, 1107, 1216, 1261, 1464, 1505
FauI CCCGC 1 cut(s) 1111
FauNDI CATATG 1 cut(s) 1367
FbaI TGATCA 1 cut(s) 745
Fnu4HI GCNGC 9 cut(s) 125, 128, 247, 390, 585, 1307, 1340, 1347, 1409
FokI GGATG 3 cut(s) 468, 690, 929
Fsp4HI GCNGC 9 cut(s) 125, 128, 247, 390, 585, 1307, 1340, 1347, 1409
FspBI CTAG 6 cut(s) 57, 206, 462, 882, 1248, 1310
FspI TGCGCA 1 cut(s) 838
GlaI GCGC 1 cut(s) 838
GluI GCNGC 9 cut(s) 125, 128, 247, 390, 585, 1307, 1340, 1347, 1409
HaeIII GGCC 3 cut(s) 281, 296, 467
HapII CCGG 3 cut(s) 1343, 1403, 1492
HhaI GCGC 1 cut(s) 839
Hin1II CATG 8 cut(s) 287, 770, 1055, 1111, 1220, 1265, 1468, 1509
Hin6I GCGC 1 cut(s) 837
HinP1I GCGC 1 cut(s) 837
HincII GTYRAC 1 cut(s) 1174
HindII GTYRAC 1 cut(s) 1174
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 4 cut(s) 905, 984, 1082, 1361
HpaII CCGG 3 cut(s) 1343, 1403, 1492
HphI GGTGA 3 cut(s) 521, 1405, 1456
Hpy166II GTNNAC 2 cut(s) 725, 1174
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 7 cut(s) 372, 714, 902, 1010, 1217, 1318, 1484
Hpy8I GTNNAC 2 cut(s) 725, 1174
HpyAV CCTTC 5 cut(s) 143, 478, 585, 751, 1113
HpyCH4III ACNGT 8 cut(s) 254, 273, 482, 521, 947, 958, 1157, 1183
HpyCH4IV ACGT 2 cut(s) 1204, 1460
HpyF10VI GCNNNNNNNGC 7 cut(s) 185, 293, 584, 843, 874, 1355, 1408
HpyF3I CTNAG 2 cut(s) 891, 1008
HpySE526I ACGT 2 cut(s) 1204, 1460
Hsp92II CATG 8 cut(s) 287, 770, 1055, 1111, 1220, 1265, 1468, 1509
HspAI GCGC 1 cut(s) 837
KroI GCCGGC 1 cut(s) 1342
KroNI GCCGGC 1 cut(s) 1344
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 5 cut(s) 118, 607, 611, 745, 1063
LmnI GCTCC 3 cut(s) 887, 1131, 1405
Lsp1109I GCAGC 8 cut(s) 114, 233, 401, 571, 1293, 1326, 1333, 1420
LweI GCATC 5 cut(s) 423, 446, 626, 668, 1066
MaeI CTAG 6 cut(s) 57, 206, 462, 882, 1248, 1310
MaeII ACGT 2 cut(s) 1204, 1460
MaeIII GTNAC 5 cut(s) 212, 267, 509, 1256, 1444
MalI GATC 5 cut(s) 120, 609, 613, 747, 1065
MboI GATC 5 cut(s) 118, 607, 611, 745, 1063
MlyI GAGTC 2 cut(s) 899, 978
MmeI TCCRAC 2 cut(s) 359, 1303
MnlI CCTC 9 cut(s) 96, 139, 250, 271, 368, 513, 686, 878, 931
Mph1103I ATGCAT 2 cut(s) 319, 641
MroNI GCCGGC 1 cut(s) 1342
MroXI GAANNNNTTC 1 cut(s) 856
MseI TTAA 1 cut(s) 803
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 2 cut(s) 124, 1193
MspI CCGG 3 cut(s) 1343, 1403, 1492
MspR9I CCNGG 1 cut(s) 1493
Mva1269I GAATGC 1 cut(s) 639
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 7 cut(s) 185, 293, 584, 843, 874, 1355, 1408
NaeI GCCGGC 1 cut(s) 1344
NciI CCSGG 1 cut(s) 1493
NcoI CCATGG 2 cut(s) 1107, 1261
NdeI CATATG 1 cut(s) 1367
NdeII GATC 5 cut(s) 118, 607, 611, 745, 1063
NgoMIV GCCGGC 1 cut(s) 1342
NheI GCTAGC 1 cut(s) 1309
NlaIII CATG 8 cut(s) 287, 770, 1055, 1111, 1220, 1265, 1468, 1509
NlaIV GGNNCC 4 cut(s) 53, 437, 1133, 1482
NmuCI GTSAC 3 cut(s) 267, 509, 1444
NsbI TGCGCA 1 cut(s) 838
NsiI ATGCAT 2 cut(s) 319, 641
PagI TCATGA 1 cut(s) 1216
PctI GAATGC 1 cut(s) 639
PdiI GCCGGC 1 cut(s) 1344
PdmI GAANNNNTTC 1 cut(s) 856
PfeI GAWTC 2 cut(s) 1082, 1361
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 9 cut(s) 126, 129, 248, 391, 586, 1308, 1341, 1348, 1410
PleI GAGTC 2 cut(s) 899, 978
PpsI GAGTC 2 cut(s) 899, 978
Ppu21I YACGTR 1 cut(s) 1205
PpuMI RGGWCCY 1 cut(s) 51
PsiI TTATAA 1 cut(s) 923
Psp5II RGGWCCY 1 cut(s) 51
PspEI GGTNACC 1 cut(s) 1256
PspN4I GGNNCC 4 cut(s) 53, 437, 1133, 1482
PspPI GGNCC 6 cut(s) 51, 280, 294, 465, 722, 1371
PspPPI RGGWCCY 1 cut(s) 51
PstI CTGCAG 1 cut(s) 848
PstNI CAGNNNCTG 3 cut(s) 127, 480, 1085
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 3 cut(s) 61, 518, 1154
RsaNI GTAC 3 cut(s) 60, 517, 1153
RseI CAYNNNNRTG 1 cut(s) 915
SaqAI TTAA 1 cut(s) 803
SatI GCNGC 9 cut(s) 125, 128, 247, 390, 585, 1307, 1340, 1347, 1409
Sau3AI GATC 5 cut(s) 118, 607, 611, 745, 1063
Sau96I GGNCC 6 cut(s) 51, 280, 294, 465, 722, 1371
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 2 cut(s) 899, 978
ScrFI CCNGG 1 cut(s) 1493
SfaNI GCATC 5 cut(s) 423, 446, 626, 668, 1066
SfcI CTRYAG 3 cut(s) 615, 844, 1068
SinI GGWCC 3 cut(s) 51, 722, 1371
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
SpeI ACTAGT 1 cut(s) 1247
SsiI CCGC 4 cut(s) 124, 192, 1104, 1349
SspI AATATT 1 cut(s) 234
SspMI CTAG 6 cut(s) 57, 206, 462, 882, 1248, 1310
StyD4I CCNGG 1 cut(s) 1491
StyI CCWWGG 4 cut(s) 393, 461, 1107, 1261
TaaI ACNGT 8 cut(s) 254, 273, 482, 521, 947, 958, 1157, 1183
TaiI ACGT 2 cut(s) 1207, 1463
TaqI TCGA 3 cut(s) 144, 610, 1178
TaqII GACCGA 1 cut(s) 1388
TatI WGTACW 2 cut(s) 59, 1152
TauI GCSGC 1 cut(s) 127
TfiI GAWTC 2 cut(s) 1082, 1361
Tru1I TTAA 1 cut(s) 803
Tru9I TTAA 1 cut(s) 803
TscAI CASTG 5 cut(s) 75, 104, 259, 755, 967
TseFI GTSAC 3 cut(s) 267, 509, 1444
TseI GCWGC 8 cut(s) 127, 246, 389, 584, 1306, 1339, 1346, 1408
Tsp45I GTSAC 3 cut(s) 267, 509, 1444
TspDTI ATGAA 4 cut(s) 585, 845, 1233, 1412
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 5 cut(s) 75, 104, 259, 755, 967
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 3 cut(s) 51, 722, 1371
XapI RAATTY 4 cut(s) 799, 852, 1139, 1379
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XmaJI CCTAGG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 856
XspI CTAG 6 cut(s) 57, 206, 462, 882, 1248, 1310
ZrmI AGTACT 1 cut(s) 61
Zsp2I ATGCAT 2 cut(s) 319, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.