pycom12g12400

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014760
Physical Location & Seq
Forward (+)
14862865 .. 14867564
4700 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2688 bp
ATGAAGCCCATTCCAAAACGCCATGAAGCCCAAGCTCATCCCAAAATTTGCAGTCAAAGCCCAGTCACTCAACGCGCTAAAAGCCACCAAAAAACGCTCGTTCTGCCTACACGAACACAAATCCAAACCCTCAAACCTCTTCTCTCTCCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTACTTTTCCGTGCTTTATCCTATCCAGGGTTTCCTCCCCTGCACTCCATTTCCTGCTTCTCGTCCTTCTCCTTCTCCGTCGCACACTGTGAGTCTGAAAATCATGAGTGAAATCGGAGAGAAAACGTGTCCGATATGCGCTGAGGAGATGGATTTGACGGATCAACAGCTGAAGCCGTGCAAATGCGGTTATGAGGTATGTGTTTGGTGTTGGCATCACATTATGGAAATGGCAGTGAATGATGGAAAGGAGGGTCGCTGTCCATTATGCCGTACACCTTATGACAAAGAAAAAATTGTTGGAGTGGCGGCAAATTGTCAAAAGTTGGTGGCTGAAATTAATGTGAAGCGGAAACAGAAGAAACCCAAGGGATCTGAAGTAAAGAAGCAGCATCTTACTGATGTTAGAGTTCTCCAACGGACCCTAGTATATGTGATTGGAATACCTCTTAACCTAGCGAGTGAAGATCTTCTCAAGCGAAGGGAGTATTTTGGCCAGTACGGAAAGGTTTTGAAGGTTTCTATCTCTCGTACAGCAAATGGGGCTATCCAACAGGCTCCGAACAACAGTTGTTGTGTGTATGTATCATACTCAAAAGAACATGAAGCAGCTCGGTGCATTCAATCAGTACACAGTTTTGTGTTAGATGGTCGATGCTTGAGGGCATCTTTTGGAACCACAAAGTACTGCCATGCATGGTTGAAAAGTGTGCCTTGCAACAATCCAGATTGTTGGTATTTGCATGATTTTGGTTCTCAGGAAGATAGTTTTACCAAGGATGAATTGCTTTCAGCCTTTGAAAGGAGTAAGGATCACCAAAATATTGGTGTTACAAATAATTTGAATCGGCATTCAGTGAAAGGCTTACCTCCCCCAACTGCTGAGAATAGCAAAGCCGAAAGGTCATCAGCTACTAATCATCCATCAAATATTACTGGGAATGAGGTTAAGAGTTATCGTGCAGATGGTGACCCCAGAACATCTATTCCTGCAATAGCACCTTGGGTCATGCAAGTCACCCCTAGTGTGCCCCCAGCAACAAGTTCTCCAGGTTCTGAAAGGCCTCTTTATCAAAATCCTGAGAAATCAATTGATCTTCATCCCCTTTCATCAGAAGTGGTACGCAATGAAAGTTCTACCCACAATGTAAGATCTGAAGAAAGCCGTGAGTGGCTAATGACCGAACAGGTTATTACCAAAAATGGTCAAGCAGCTATATCAAATACAACTTCTGGAGCTCTAGCAGAAAGAGCTCCGGTCAGTGAAACTTCCAACAAAAACTTATGTTGTCCATCACAAAACAATGGCATTGAGCGGGGTAGCAGAGCAACATCCACCAACTTGATCAGCAACAAAAACAGTCATTGTTCATCATCAAACTTATCAGATAAATCCATTGATATCCAAGGCAGTAGCGTTGCTGAATTGTATAGAACATATGAACCTACCAGTGTGTTACCTGAGATGGGATTAGGGAAGTACTCGAGAGGGTGTGAAACCAATAAGCCCAGCATTCACCGCAGTCTTTCTCCAGATATTGGAGAGAGTGGCATAATCTCAAGTATTTTGACCATGGACTTGGATGCACCTGATGGTTCTTTATCTCCTCTTCACAATCTTGTAAAGTTGTTGAGTGAAACTGATAAAGAGTGCAGTCTCCTCAGAGTACAAAATTCACGAAAACTGCTTGATAAAAAGCAGTCCAGGTTCTCATTTGCTCAACAAGAGGATTCTTGTGATAATGATATATGGAATGCATCCAAGGACTACGCCTTATCTGGTTTGATGGGCAACAAGGAGTACTTCGTAGATGTAGAGTCTAATAAGTTTCTTGGGGTTCCTTCTTTTACGTCTTCTAAAGTATCAGAAGTTCCTCGTTCAATTGCGCCAGGATTTTCAGTGCCAAGTAGAGCACCACCTCCAGGGTTTTCTTCTCATTTGAGGCCTAGGAGGGTGGATCAGGCTTTTGACAGTTCAGTGAATCACTTGTTGCAACCATCATGTATACCAACTGGTTATTCTGGTATTAATGGGAATGTCGAGATCAATGATCCAGCAATTCTGGAAGTCAGTGAGGAACTATTGGCAAGAGGGTTGAGTAAGAGGGCAAATTTATCTCAGTTTAACTCTTCTGAGCCAGATGCTAGGCTCCATATGCTGAGACAGCAGTCAACTTCTGCCCCACAGAACTTAGGATGTCAAGACCACTTCATGAATAGTTACTCTTACCTAAATGATGCACAAAGAATTACTACACAGCAGCCAGATCAATTCCAGCCCAATCCATCTTCTTCATTTGTGCAACCCACCAGTCAACACTTCAGTGACATGCATTTATCAAATAATGATTGGGTGCAATGGAATCGGGTCAATAGTGTTGCCGATCTTGGTATATCAGATCTACTGAGAAACGATCGACTGGGATTTAACAAGCTCATTCCCTGTTATGAGAATAAGCAGTCTCCAACAAGTTACTTTAGTCATCTAAACAACAGATCATTTGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
RRM_1 zf-RING_4

Protein Analysis

896

Amino Acids

99.36

Weight (kDa)

8.39

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 102 - 154 2.1e-18 RING/Ubox like zinc-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g15190
malus_domestica MD12G1125800.v1.1
prunus_persica Prupe.6G242300_v2.0.a1 Prupe.6G242300_v2.0.a1
pyrus_communis pycom12g12400
rosa_chinensis RchiOBHm_Chr3g0468541
rosa_laevigata RLG00000024424
rosa_multiflora Rmu_sc0008735.1_g000001 Rmu_sc0009516.1_g000009
rosa_roxburghii Rroxscaffold_6G00412710
rosa_rugosa Rorug03G0095700 Rorug03G0095800 Rorug03G0095800
rosa_samantha Rh3AG144200 Rh3BG166400 Rh3DG165800
rosa_wichuraiana Rw3G013610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1715
AccBSI CCGCTC 1 cut(s) 1492
AccI GTMKAC 1 cut(s) 2182
AccII CGCG 1 cut(s) 75
AciI CCGC 5 cut(s) 363, 485, 526, 1492, 1696
AclWI GGATC 5 cut(s) 345, 556, 996, 2142, 2222
AcoI YGGCCR 1 cut(s) 670
AcsI RAATTY 3 cut(s) 45, 1849, 2287
AcuI CTGAAG 4 cut(s) 368, 573, 1353, 2482
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 9 cut(s) 451, 677, 709, 807, 863, 1299, 1658, 1845, 1979
AfiI CCNNNNNNNGG 5 cut(s) 203, 978, 1643, 1715, 2099
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 7 cut(s) 691, 800, 880, 977, 1021, 2058, 2681
AjnI CCWGG 5 cut(s) 201, 1225, 1880, 2065, 2098
AjuI GAANNNNNNNTTGG 4 cut(s) 459, 491, 2179, 2211
AleI CACNNNNGTG 1 cut(s) 2499
AloI GAACNNNNNNTCC 2 cut(s) 1147, 1179
AluBI AGCT 8 cut(s) 35, 346, 788, 1088, 1391, 1415, 1430, 2611
AluI AGCT 8 cut(s) 35, 346, 788, 1088, 1391, 1415, 1430, 2611
Alw21I GWGCWC 3 cut(s) 1417, 1432, 2092
Alw26I GTCTC 3 cut(s) 1838, 2332, 2643
AlwI GGATC 5 cut(s) 345, 556, 996, 2142, 2222
AlwNI CAGNNNCTG 1 cut(s) 1232
Ama87I CYCGRG 1 cut(s) 1660
AoxI GGCC 3 cut(s) 670, 1238, 2120
ApeKI GCWGC 4 cut(s) 565, 785, 1388, 2437
ApoI RAATTY 3 cut(s) 45, 1849, 2287
AseI ATTAAT 2 cut(s) 516, 2205
Asp700I GAANNNNTTC 1 cut(s) 645
AspA2I CCTAGG 1 cut(s) 2123
AspLEI GCGC 3 cut(s) 77, 317, 2065
AspS9I GGNCC 1 cut(s) 597
AsuHPI GGTGA 4 cut(s) 983, 1157, 1186, 1685
AvaI CYCGRG 1 cut(s) 1660
AvaII GGWCC 1 cut(s) 597
AvrII CCTAGG 1 cut(s) 2123
BaeGI GKGCMC 1 cut(s) 1209
BalI TGGCCA 1 cut(s) 672
BanII GRGCYC 2 cut(s) 1417, 1432
BbsI GAAGAC 1 cut(s) 2022
Bbv12I GWGCWC 3 cut(s) 1417, 1432, 2092
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 4 cut(s) 577, 797, 1400, 2449
BceAI ACGGC 3 cut(s) 337, 432, 1326
BcgI CGANNNNNNTGC 2 cut(s) 2522, 2556
BciT130I CCWGG 5 cut(s) 203, 1227, 1882, 2067, 2100
BclI TGATCA 1 cut(s) 1521
BcoDI GTCTC 3 cut(s) 1838, 2332, 2643
BfaI CTAG 6 cut(s) 602, 632, 1200, 1418, 2124, 2322
BglII AGATCT 3 cut(s) 643, 1328, 2575
BisI GCNGC 5 cut(s) 486, 566, 786, 1389, 2438
BlnI CCTAGG 1 cut(s) 2123
BlsI GCNGC 5 cut(s) 487, 567, 787, 1390, 2439
BmcAI AGTACT 3 cut(s) 863, 1658, 1979
Bme1390I CCNGG 5 cut(s) 203, 1227, 1882, 2067, 2100
Bme18I GGWCC 1 cut(s) 597
BmeT110I CYCGRG 1 cut(s) 1660
BmgT120I GGNCC 1 cut(s) 597
BmiI GGNNCC 5 cut(s) 599, 735, 853, 2016, 2327
BmrFI CCNGG 5 cut(s) 203, 1227, 1882, 2067, 2100
BmrI ACTGGG 3 cut(s) 56, 1122, 2606
BmsI GCATC 8 cut(s) 400, 577, 821, 851, 1750, 1943, 2308, 2404
BmuI ACTGGG 3 cut(s) 56, 1122, 2606
BoxI GACNNNNGTC 1 cut(s) 2344
BpiI GAAGAC 1 cut(s) 2022
BpmI CTGGAG 4 cut(s) 1209, 1431, 1692, 2082
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 3 cut(s) 635, 856, 1720
BsaBI GATNNNNATC 1 cut(s) 1275
BsaJI CCNNGG 9 cut(s) 202, 543, 951, 1178, 1582, 1749, 1938, 2099, 2123
BsaWI WCCGGW 1 cut(s) 1432
BsaXI ACNNNNNCTCC 2 cut(s) 1207, 1237
Bsc4I CCNNNNNNNGG 5 cut(s) 203, 978, 1643, 1715, 2099
Bse1I ACTGG 7 cut(s) 62, 673, 1117, 1626, 2194, 2487, 2601
Bse3DI GCAATG 2 cut(s) 1309, 2540
Bse8I GATNNNNATC 1 cut(s) 1275
BseBI CCWGG 5 cut(s) 203, 1227, 1882, 2067, 2100
BseDI CCNNGG 9 cut(s) 202, 543, 951, 1178, 1582, 1749, 1938, 2099, 2123
BseGI GGATG 8 cut(s) 37, 961, 1096, 1276, 1508, 1765, 1934, 2378
BseJI GATNNNNATC 1 cut(s) 1275
BseLI CCNNNNNNNGG 5 cut(s) 203, 978, 1643, 1715, 2099
BseMI GCAATG 2 cut(s) 1309, 2540
BseNI ACTGG 7 cut(s) 62, 673, 1117, 1626, 2194, 2487, 2601
BseRI GAGGAG 4 cut(s) 138, 335, 1773, 1826
BseSI GKGCMC 1 cut(s) 1209
BseXI GCAGC 4 cut(s) 577, 797, 1400, 2449
BseYI CCCAGC 2 cut(s) 1210, 1685
BsgI GTGCAG 3 cut(s) 202, 1158, 1849
Bsh1236I CGCG 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 2593
BshFI GGCC 3 cut(s) 672, 1240, 2122
BsiEI CGRYCG 1 cut(s) 2593
BsiHKAI GWGCWC 3 cut(s) 1417, 1432, 2092
BsiHKCI CYCGRG 1 cut(s) 1660
BsiSI CCGG 1 cut(s) 1433
BslI CCNNNNNNNGG 5 cut(s) 203, 978, 1643, 1715, 2099
BsmAI GTCTC 3 cut(s) 1838, 2332, 2643
BsmI GAATGC 4 cut(s) 795, 1027, 1689, 1936
BsnI GGCC 3 cut(s) 672, 1240, 2122
BsoBI CYCGRG 1 cut(s) 1660
Bsp1286I GDGCHC 4 cut(s) 1209, 1417, 1432, 2092
Bsp19I CCATGG 1 cut(s) 1749
BspACI CCGC 5 cut(s) 363, 485, 526, 1492, 1696
BspANI GGCC 3 cut(s) 672, 1240, 2122
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 2 cut(s) 279, 2388
BspLI GGNNCC 5 cut(s) 599, 735, 853, 2016, 2327
BspPI GGATC 5 cut(s) 345, 556, 996, 2142, 2222
BsrBI CCGCTC 1 cut(s) 1492
BsrDI GCAATG 2 cut(s) 1309, 2540
BsrI ACTGG 7 cut(s) 62, 673, 1117, 1626, 2194, 2487, 2601
BssECI CCNNGG 9 cut(s) 202, 543, 951, 1178, 1582, 1749, 1938, 2099, 2123
BssNAI GTATAC 1 cut(s) 2183
BssT1I CCWWGG 7 cut(s) 543, 951, 1178, 1582, 1749, 1938, 2123
Bst1107I GTATAC 1 cut(s) 2183
Bst2UI CCWGG 5 cut(s) 203, 1227, 1882, 2067, 2100
Bst4CI ACNGT 5 cut(s) 265, 746, 812, 1538, 2150
Bst6I CTCTTC 3 cut(s) 144, 1791, 2311
BstDSI CCRYGG 1 cut(s) 1749
BstEII GGTNACC 1 cut(s) 1145
BstENI CCTNNNNNAGG 1 cut(s) 976
BstF5I GGATG 8 cut(s) 37, 961, 1096, 1276, 1508, 1765, 1934, 2378
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 3 cut(s) 77, 317, 2065
BstMAI GTCTC 3 cut(s) 1838, 2332, 2643
BstMCI CGRYCG 1 cut(s) 2593
BstMWI GCNNNNNNNGC 8 cut(s) 57, 81, 103, 719, 1427, 1695, 2332, 2341
BstNI CCWGG 5 cut(s) 203, 1227, 1882, 2067, 2100
BstNSI RCATGY 1 cut(s) 2509
BstPAI GACNNNNGTC 1 cut(s) 2344
BstPI GGTNACC 1 cut(s) 1145
BstSCI CCNGG 5 cut(s) 201, 1225, 1880, 2065, 2098
BstSLI GKGCMC 1 cut(s) 1209
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 4 cut(s) 577, 797, 1400, 2449
BstV2I GAAGAC 1 cut(s) 2022
BstX2I RGATCY 4 cut(s) 548, 643, 1328, 2575
BstXI CCANNNNNNTGG 3 cut(s) 909, 1001, 1756
BstYI RGATCY 4 cut(s) 548, 643, 1328, 2575
BstZ17I GTATAC 1 cut(s) 2183
BsuRI GGCC 3 cut(s) 672, 1240, 2122
BtgI CCRYGG 1 cut(s) 1749
BtsCI GGATG 8 cut(s) 37, 961, 1096, 1276, 1508, 1765, 1934, 2378
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 9 cut(s) 261, 417, 1038, 1444, 1633, 2082, 2160, 2254, 2506
CaiI CAGNNNCTG 1 cut(s) 1232
CciI TCATGA 2 cut(s) 279, 2388
CfoI GCGC 3 cut(s) 77, 317, 2065
Cfr13I GGNCC 1 cut(s) 597
Csp6I GTAC 9 cut(s) 450, 676, 708, 806, 862, 1298, 1657, 1844, 1978
CviQI GTAC 9 cut(s) 450, 676, 708, 806, 862, 1298, 1657, 1844, 1978
DraIII CACNNNGTG 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 670
Eam1104I CTCTTC 3 cut(s) 144, 1791, 2311
EarI CTCTTC 3 cut(s) 144, 1791, 2311
Ecl136II GAGCTC 2 cut(s) 1415, 1430
Eco130I CCWWGG 7 cut(s) 543, 951, 1178, 1582, 1749, 1938, 2123
Eco147I AGGCCT 2 cut(s) 1240, 2122
Eco24I GRGCYC 2 cut(s) 1417, 1432
Eco32I GATATC 1 cut(s) 1579
Eco47I GGWCC 1 cut(s) 597
Eco53kI GAGCTC 2 cut(s) 1415, 1430
Eco57I CTGAAG 4 cut(s) 368, 573, 1353, 2482
Eco88I CYCGRG 1 cut(s) 1660
Eco91I GGTNACC 1 cut(s) 1145
EcoICRI GAGCTC 2 cut(s) 1415, 1430
EcoNI CCTNNNNNAGG 1 cut(s) 976
EcoO65I GGTNACC 1 cut(s) 1145
EcoRII CCWGG 5 cut(s) 201, 1225, 1880, 2065, 2098
EcoRV GATATC 1 cut(s) 1579
EcoT14I CCWWGG 7 cut(s) 543, 951, 1178, 1582, 1749, 1938, 2123
EcoT22I ATGCAT 3 cut(s) 874, 1936, 2511
EcoT38I GRGCYC 2 cut(s) 1417, 1432
ErhI CCWWGG 7 cut(s) 543, 951, 1178, 1582, 1749, 1938, 2123
FalI AAGNNNNNCTT 4 cut(s) 874, 906, 1964, 1996
FauI CCCGC 1 cut(s) 1485
FauNDI CATATG 2 cut(s) 1615, 2331
FbaI TGATCA 1 cut(s) 1521
FblI GTMKAC 1 cut(s) 2182
Fnu4HI GCNGC 5 cut(s) 486, 566, 786, 1389, 2438
FokI GGATG 8 cut(s) 24, 968, 1083, 1263, 1495, 1772, 1921, 2385
FriOI GRGCYC 2 cut(s) 1417, 1432
Fsp4HI GCNGC 5 cut(s) 486, 566, 786, 1389, 2438
FspBI CTAG 6 cut(s) 602, 632, 1200, 1418, 2124, 2322
GlaI GCGC 3 cut(s) 76, 316, 2064
GluI GCNGC 5 cut(s) 486, 566, 786, 1389, 2438
GsaI CCCAGC 2 cut(s) 1214, 1689
GsuI CTGGAG 4 cut(s) 1209, 1431, 1692, 2082
HaeIII GGCC 3 cut(s) 672, 1240, 2122
HapII CCGG 1 cut(s) 1433
HhaI GCGC 3 cut(s) 77, 317, 2065
Hin6I GCGC 3 cut(s) 75, 315, 2063
HinP1I GCGC 3 cut(s) 75, 315, 2063
HincII GTYRAC 2 cut(s) 2349, 2492
HindII GTYRAC 2 cut(s) 2349, 2492
HinfI GANTC 6 cut(s) 268, 1021, 1907, 1994, 2158, 2539
HpaII CCGG 1 cut(s) 1433
HphI GGTGA 4 cut(s) 983, 1157, 1186, 1685
Hpy166II GTNNAC 5 cut(s) 452, 808, 2183, 2349, 2492
Hpy8I GTNNAC 5 cut(s) 452, 808, 2183, 2349, 2492
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 5 cut(s) 252, 258, 651, 685, 2028
HpyCH4III ACNGT 5 cut(s) 265, 746, 812, 1538, 2150
HpyCH4IV ACGT 2 cut(s) 302, 2027
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 81, 103, 719, 1427, 1695, 2332, 2341
HpySE526I ACGT 2 cut(s) 302, 2027
HspAI GCGC 3 cut(s) 75, 315, 2063
Ksp22I TGATCA 1 cut(s) 1521
LmnI GCTCC 4 cut(s) 739, 1412, 1435, 2331
Lsp1109I GCAGC 4 cut(s) 577, 797, 1400, 2449
LweI GCATC 8 cut(s) 400, 577, 821, 851, 1750, 1943, 2308, 2404
MaeI CTAG 6 cut(s) 602, 632, 1200, 1418, 2124, 2322
MaeII ACGT 2 cut(s) 302, 2027
MaeIII GTNAC 8 cut(s) 64, 1006, 1145, 1192, 1632, 2396, 2501, 2648
MbiI CCGCTC 1 cut(s) 1492
MfeI CAATTG 2 cut(s) 1266, 2058
MflI RGATCY 4 cut(s) 548, 643, 1328, 2575
MhlI GDGCHC 4 cut(s) 1209, 1417, 1432, 2092
MlsI TGGCCA 1 cut(s) 672
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 2 cut(s) 277, 2003
MmeI TCCRAC 5 cut(s) 457, 616, 751, 1473, 2666
Mox20I TGGCCA 1 cut(s) 672
Mph1103I ATGCAT 3 cut(s) 874, 1936, 2511
MroXI GAANNNNTTC 1 cut(s) 645
MscI TGGCCA 1 cut(s) 672
MseI TTAA 6 cut(s) 516, 627, 1125, 2205, 2301, 2604
MslI CAYNNNNRTG 1 cut(s) 2499
Msp20I TGGCCA 1 cut(s) 672
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 1 cut(s) 1433
MspR9I CCNGG 5 cut(s) 203, 1227, 1882, 2067, 2100
MunI CAATTG 2 cut(s) 1266, 2058
Mva1269I GAATGC 4 cut(s) 795, 1027, 1689, 1936
MvaI CCWGG 5 cut(s) 203, 1227, 1882, 2067, 2100
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 8 cut(s) 57, 81, 103, 719, 1427, 1695, 2332, 2341
NcoI CCATGG 1 cut(s) 1749
NdeI CATATG 2 cut(s) 1615, 2331
NlaIV GGNNCC 5 cut(s) 599, 735, 853, 2016, 2327
NmuCI GTSAC 4 cut(s) 64, 1145, 1192, 2501
NsiI ATGCAT 3 cut(s) 874, 1936, 2511
NspI RCATGY 1 cut(s) 2509
OliI CACNNNNGTG 1 cut(s) 2499
PaeR7I CTCGAG 1 cut(s) 1660
PagI TCATGA 2 cut(s) 279, 2388
PceI AGGCCT 2 cut(s) 1240, 2122
PctI GAATGC 4 cut(s) 795, 1027, 1689, 1936
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 4 cut(s) 1021, 1907, 2158, 2539
PflMI CCANNNNNTGG 1 cut(s) 1715
PkrI GCNGC 5 cut(s) 487, 567, 787, 1390, 2439
Ple19I CGATCG 1 cut(s) 2593
PleI GAGTC 2 cut(s) 276, 2002
PpsI GAGTC 2 cut(s) 276, 2002
PshAI GACNNNNGTC 1 cut(s) 2344
PshBI ATTAAT 2 cut(s) 516, 2205
Psp124BI GAGCTC 2 cut(s) 1417, 1432
Psp6I CCWGG 5 cut(s) 201, 1225, 1880, 2065, 2098
PspEI GGTNACC 1 cut(s) 1145
PspFI CCCAGC 2 cut(s) 1210, 1685
PspGI CCWGG 5 cut(s) 201, 1225, 1880, 2065, 2098
PspN4I GGNNCC 5 cut(s) 599, 735, 853, 2016, 2327
PspPI GGNCC 1 cut(s) 597
PsrI GAACNNNNNNTAC 2 cut(s) 2031, 2063
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 4 cut(s) 548, 643, 1328, 2575
PvuI CGATCG 1 cut(s) 2593
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 9 cut(s) 451, 677, 709, 807, 863, 1299, 1658, 1845, 1979
RsaNI GTAC 9 cut(s) 450, 676, 708, 806, 862, 1298, 1657, 1844, 1978
RseI CAYNNNNRTG 1 cut(s) 2499
SacI GAGCTC 2 cut(s) 1417, 1432
SaqAI TTAA 6 cut(s) 516, 627, 1125, 2205, 2301, 2604
SatI GCNGC 5 cut(s) 486, 566, 786, 1389, 2438
Sau96I GGNCC 1 cut(s) 597
ScaI AGTACT 3 cut(s) 863, 1658, 1979
SchI GAGTC 2 cut(s) 277, 2003
ScrFI CCNGG 5 cut(s) 203, 1227, 1882, 2067, 2100
SduI GDGCHC 4 cut(s) 1209, 1417, 1432, 2092
SfaNI GCATC 8 cut(s) 400, 577, 821, 851, 1750, 1943, 2308, 2404
Sfr274I CTCGAG 1 cut(s) 1660
SinI GGWCC 1 cut(s) 597
SlaI CTCGAG 1 cut(s) 1660
SmiMI CAYNNNNRTG 1 cut(s) 2499
SmlI CTYRAG 4 cut(s) 650, 835, 1660, 1735
SmoI CTYRAG 4 cut(s) 650, 835, 1660, 1735
SseBI AGGCCT 2 cut(s) 1240, 2122
SsiI CCGC 5 cut(s) 363, 485, 526, 1492, 1696
SspI AATATT 2 cut(s) 1000, 1108
SspMI CTAG 6 cut(s) 602, 632, 1200, 1418, 2124, 2322
SstI GAGCTC 2 cut(s) 1417, 1432
StuI AGGCCT 2 cut(s) 1240, 2122
StyD4I CCNGG 5 cut(s) 201, 1225, 1880, 2065, 2098
StyI CCWWGG 7 cut(s) 543, 951, 1178, 1582, 1749, 1938, 2123
TaaI ACNGT 5 cut(s) 265, 746, 812, 1538, 2150
TaiI ACGT 2 cut(s) 305, 2030
TaqI TCGA 4 cut(s) 829, 1661, 2217, 2593
TaqII GACCGA 1 cut(s) 1373
TatI WGTACW 5 cut(s) 805, 861, 1656, 1843, 1977
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 4 cut(s) 1021, 1907, 2158, 2539
Tru1I TTAA 6 cut(s) 516, 627, 1125, 2205, 2301, 2604
Tru9I TTAA 6 cut(s) 516, 627, 1125, 2205, 2301, 2604
TscAI CASTG 9 cut(s) 268, 417, 1038, 1444, 1633, 2082, 2160, 2254, 2506
TseFI GTSAC 4 cut(s) 64, 1145, 1192, 2501
TseI GCWGC 4 cut(s) 565, 785, 1388, 2437
Tsp45I GTSAC 4 cut(s) 64, 1145, 1192, 2501
TspGWI ACGGA 5 cut(s) 175, 243, 350, 610, 693
TspRI CASTG 9 cut(s) 268, 417, 1038, 1444, 1633, 2082, 2160, 2254, 2506
Van91I CCANNNNNTGG 1 cut(s) 1715
VpaK11BI GGWCC 1 cut(s) 597
VspI ATTAAT 2 cut(s) 516, 2205
XagI CCTNNNNNAGG 1 cut(s) 976
XapI RAATTY 3 cut(s) 45, 1849, 2287
XceI RCATGY 1 cut(s) 2509
XhoI CTCGAG 1 cut(s) 1660
XmaJI CCTAGG 1 cut(s) 2123
XmiI GTMKAC 1 cut(s) 2182
XmnI GAANNNNTTC 1 cut(s) 645
XspI CTAG 6 cut(s) 602, 632, 1200, 1418, 2124, 2322
ZrmI AGTACT 3 cut(s) 863, 1658, 1979
Zsp2I ATGCAT 3 cut(s) 874, 1936, 2511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.