pycom12g14800

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007670
Physical Location & Seq
Forward (+)
17312467 .. 17313002
536 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGTCACTCCAGAGCATATACTTCTCGGAGCACAGCGGTGGCAAATGGGTGGATGCATATGATTTGGAGTACACAGACGGGAACAAAGCCATTGTCTACTCATCCAAAAATGAACACGCGAGCTACCCTCATCCTGGGACTTACCTTCAGGGGTCAGACAAGCTAGGCATCGGAATAAGGAACGATGCTGCTCGCAGCAACTTATCTGTGGATTCAAGCATTCGGTATGAGATTGTTGCAGCCGAGTATCTTGGAGACGGGGTTGTGAGTGAGCCGGATTGGCTGCAGTTTATGAGAGATTGGGGTCCAACAATAGTGTACAACTCAAGAACTGAGCTTGATAAGGTTATCAATGTCTTGCCTGTGGTGTTTAAGCACAGATTGGAGAGCTTCCAGTTGAGCTTTATGGGGAGGAAGGTCCTACTGGACCAAAGGAGAAGAACAATTGGGTGGGAGATGAGAGAAGCTAGGGTTTTGCTTGGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

163

Amino Acids

18.51

Weight (kDa)

6.08

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps62 PF06101 3 - 131 1.7e-60 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 96
AccII CGCG 1 cut(s) 119
AciI CCGC 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 131
AfaI GTAC 2 cut(s) 71, 320
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 1 cut(s) 216
AjnI CCWGG 1 cut(s) 133
AleI CACNNNNGTG 1 cut(s) 36
AluBI AGCT 6 cut(s) 123, 163, 337, 390, 402, 467
AluI AGCT 6 cut(s) 123, 163, 337, 390, 402, 467
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 249
ApeKI GCWGC 4 cut(s) 188, 195, 239, 283
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
AspS9I GGNCC 3 cut(s) 305, 418, 427
AvaII GGWCC 3 cut(s) 305, 418, 427
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 4 cut(s) 175, 207, 251, 270
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 2 cut(s) 164, 468
BfmI CTRYAG 1 cut(s) 284
BglI GCCNNNNNGGC 1 cut(s) 280
BisI GCNGC 4 cut(s) 189, 196, 240, 284
BlsI GCNGC 4 cut(s) 190, 197, 241, 285
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 3 cut(s) 305, 418, 427
BmgT120I GGNCC 3 cut(s) 305, 418, 427
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 3 cut(s) 43, 175, 177
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpuEI CTTGAG 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 394, 429
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 3 cut(s) 58, 101, 130
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 394, 429
BseXI GCAGC 4 cut(s) 175, 207, 251, 270
Bsh1236I CGCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 249
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 33
Bsp1407I TGTACA 1 cut(s) 318
BspACI CCGC 1 cut(s) 36
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 288
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 2 cut(s) 394, 429
BssECI CCNNGG 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 121, 193
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 58, 101, 130
BstFNI CGCG 1 cut(s) 119
BstMAI GTCTC 1 cut(s) 249
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 284
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 4 cut(s) 175, 207, 251, 270
BtsCI GGATG 3 cut(s) 58, 101, 130
Cac8I GCNNGC 2 cut(s) 121, 193
Cfr13I GGNCC 3 cut(s) 305, 418, 427
Csp6I GTAC 2 cut(s) 70, 319
CviQI GTAC 2 cut(s) 70, 319
DdeI CTNAG 1 cut(s) 333
Eco47I GGWCC 3 cut(s) 305, 418, 427
Eco57I CTGAAG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 133
EcoT22I ATGCAT 1 cut(s) 58
Esp3I CGTCTC 1 cut(s) 249
FaiI YATR 7 cut(s) 17, 19, 58, 60, 228, 293, 407
FaqI GGGAC 1 cut(s) 151
FauNDI CATATG 1 cut(s) 58
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 4 cut(s) 189, 196, 240, 284
FokI GGATG 3 cut(s) 65, 88, 117
Fsp4HI GCNGC 4 cut(s) 189, 196, 240, 284
FspBI CTAG 2 cut(s) 164, 468
GluI GCNGC 4 cut(s) 189, 196, 240, 284
HapII CCGG 1 cut(s) 275
HinfI GANTC 1 cut(s) 212
HpaII CCGG 1 cut(s) 275
Hpy166II GTNNAC 3 cut(s) 72, 97, 319
Hpy188I TCNGA 3 cut(s) 28, 157, 173
Hpy188III TCNNGA 2 cut(s) 10, 327
Hpy8I GTNNAC 3 cut(s) 72, 97, 319
HpyAV CCTTC 2 cut(s) 155, 409
HpyCH4V TGCA 3 cut(s) 56, 239, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 1 cut(s) 333
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 8 cut(s) 23, 120, 134, 147, 288, 375, 407, 410
Lsp1109I GCAGC 4 cut(s) 175, 207, 251, 270
LweI GCATC 3 cut(s) 43, 175, 177
MaeI CTAG 2 cut(s) 164, 468
MaeIII GTNAC 1 cut(s) 3
MboII GAAGA 1 cut(s) 450
MfeI CAATTG 1 cut(s) 444
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 444
MmeI TCCRAC 1 cut(s) 332
MnlI CCTC 2 cut(s) 138, 405
Mph1103I ATGCAT 1 cut(s) 58
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 280
NdeI CATATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 306
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 36
PctI GAATGC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 4 cut(s) 190, 197, 241, 285
PpuMI RGGWCCY 1 cut(s) 418
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 3 cut(s) 305, 418, 427
PspPPI RGGWCCY 1 cut(s) 418
PstI CTGCAG 1 cut(s) 288
RsaI GTAC 2 cut(s) 71, 320
RsaNI GTAC 2 cut(s) 70, 319
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 4 cut(s) 189, 196, 240, 284
Sau96I GGNCC 3 cut(s) 305, 418, 427
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 33
SetI ASST 9 cut(s) 125, 147, 165, 339, 348, 392, 404, 420, 469
SfaNI GCATC 3 cut(s) 43, 175, 177
SfcI CTRYAG 1 cut(s) 284
SinI GGWCC 3 cut(s) 305, 418, 427
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
Sse9I AATT 1 cut(s) 444
SsiI CCGC 1 cut(s) 36
SspMI CTAG 2 cut(s) 164, 468
StyD4I CCNGG 1 cut(s) 133
TasI AATT 1 cut(s) 444
TatI WGTACW 2 cut(s) 69, 318
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 4 cut(s) 188, 195, 239, 283
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 126
VpaK11BI GGWCC 3 cut(s) 305, 418, 427
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 2 cut(s) 164, 468
Zsp2I ATGCAT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.