pycom12g15880

Domain of unknown function (DUF3453)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007393
Physical Location & Seq
Forward (+)
18318609 .. 18321050
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 855 bp
ATGCCCCACTTTGCGATAAATTATTTGGATCGATCCCAGTCTGGTCCTCCTCTGGCTCCAGCTGAAATCTTGATTGCTATCCACGGAATTGATCCCGACAGAGATGGAATTCCACTAAAGAAGGTCACAGATGCATGCAATGCCTGCTTTGAGCAGAGGCAGATATTCACCCAACAAGTTCTTGCCAAAGTTTTGAATCAGTTGGTTGAGCAGATTCCTCTTCCTTTACTGTTCATGCGTACGGTATTACAAGCCATAGGTGCCTTTCCTGCACTGGTGGATTTTATAATGGAGATCCTGTCTCGGCTTGTGAGCAAGCAGATATGGAAATACCCGAAGTTGTGGGTGGGATTCTTAAAGTGTACGTTCTTGACAAAACCTCAGTCATTTGCCGTGCTGCTTCAGCTACCTCCAGCACAGCTAGAAAACGCACTGAAGAGAACTGCGGCTCTGAAAGGACCGTTGGTTGCTCATGCTAGTCAACCGGACATCCGATCTTCCCTTCCAAGGTCGATATTGGTAGTTTTGGGAATTTCTCAAGCCACGGCACAAACAAGTCAGTCTCAAGCTGCGGCACAGACAAGTCAGTCTCAAGCTGCGGCACAGACAAGTCAGTCTCAGGCCGCGGCACATACAAGTCAGTCTCAGGCCGAGGCACATACAAGTCAGTCTCAGGCCGAGGCATATACAAGTCAGTCTCAGGCCGAGGCACAGACAAGTCAGTCTAAGCCTGAGGCACAGACAGGTCAGTCTCAGGCCGAGGCACAGTCAAGTCAGTCCCAGGCTGGGGATGCAAGCAACTCGGAAAACGACGCAGTCACAGAGAAACCAAAGGAATCATCTAGTGCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04530 map03015 map04530
Pfam Domains
Protein Families
DUF3453 Symplekin_C

Protein Analysis

285

Amino Acids

30.44

Weight (kDa)

6.52

Isoelectric Point (pI)

66.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Symplekin_C PF12295 12 - 147 1.2e-54 Symplekin tight junction protein C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
AasI GACNNNNNNGTC 4 cut(s) 586, 613, 721, 748
AccB1I GGYRCC 1 cut(s) 260
AccII CGCG 1 cut(s) 626
AciI CCGC 5 cut(s) 446, 572, 599, 624, 626
AclWI GGATC 4 cut(s) 27, 36, 86, 289
AcsI RAATTY 2 cut(s) 108, 531
AcuI CTGAAG 2 cut(s) 386, 455
AfaI GTAC 2 cut(s) 241, 364
AfiI CCNNNNNNNGG 3 cut(s) 507, 786, 787
AgsI TTSAA 1 cut(s) 196
AjnI CCWGG 1 cut(s) 780
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AluBI AGCT 5 cut(s) 62, 406, 421, 569, 596
AluI AGCT 5 cut(s) 62, 406, 421, 569, 596
Alw26I GTCTC 8 cut(s) 306, 567, 594, 621, 648, 675, 702, 756
AlwI GGATC 4 cut(s) 27, 36, 86, 289
AoxI GGCC 5 cut(s) 621, 648, 675, 702, 756
ApeKI GCWGC 3 cut(s) 397, 569, 596
ApoI RAATTY 2 cut(s) 108, 531
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspS9I GGNCC 2 cut(s) 44, 458
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 2 cut(s) 44, 458
AxyI CCTNAGG 1 cut(s) 732
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 3 cut(s) 384, 556, 583
BccI CCATC 1 cut(s) 98
BceAI ACGGC 2 cut(s) 377, 561
BcgI CGANNNNNNTGC 2 cut(s) 783, 817
BciT130I CCWGG 1 cut(s) 782
BcoDI GTCTC 8 cut(s) 306, 567, 594, 621, 648, 675, 702, 756
BfaI CTAG 4 cut(s) 422, 477, 843, 849
BisI GCNGC 8 cut(s) 398, 447, 570, 573, 597, 600, 624, 627
BlsI GCNGC 8 cut(s) 399, 448, 571, 574, 598, 601, 625, 628
Bme1390I CCNGG 1 cut(s) 782
Bme18I GGWCC 2 cut(s) 44, 458
BmgT120I GGNCC 2 cut(s) 44, 458
BmiI GGNNCC 2 cut(s) 57, 262
BmrFI CCNGG 1 cut(s) 782
BmrI ACTGGG 1 cut(s) 31
BmsI GCATC 2 cut(s) 121, 781
BmuI ACTGGG 1 cut(s) 31
BpmI CTGGAG 2 cut(s) 42, 396
BpuEI CTTGAG 3 cut(s) 522, 549, 576
Bsa29I ATCGAT 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 9 cut(s) 82, 506, 543, 624, 651, 678, 705, 759, 780
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 3 cut(s) 507, 786, 787
Bse1I ACTGG 2 cut(s) 37, 279
Bse21I CCTNAGG 1 cut(s) 732
Bse3DI GCAATG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 1 cut(s) 782
BseCI ATCGAT 1 cut(s) 31
BseDI CCNNGG 9 cut(s) 82, 506, 543, 624, 651, 678, 705, 759, 780
BseGI GGATG 2 cut(s) 489, 796
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 3 cut(s) 507, 786, 787
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 7 cut(s) 395, 632, 659, 686, 713, 723, 767
BseNI ACTGG 2 cut(s) 37, 279
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 3 cut(s) 384, 556, 583
BseYI CCCAGC 1 cut(s) 785
BsgI GTGCAG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 626
BshFI GGCC 5 cut(s) 623, 650, 677, 704, 758
BshNI GGYRCC 1 cut(s) 260
BshVI ATCGAT 1 cut(s) 31
BsiSI CCGG 1 cut(s) 485
BsiWI CGTACG 1 cut(s) 239
BslFI GGGAC 1 cut(s) 763
BslI CCNNNNNNNGG 3 cut(s) 507, 786, 787
BsmAI GTCTC 8 cut(s) 306, 567, 594, 621, 648, 675, 702, 756
BsmFI GGGAC 1 cut(s) 763
BsnI GGCC 5 cut(s) 623, 650, 677, 704, 758
Bsp143I GATC 5 cut(s) 28, 32, 91, 294, 494
BspACI CCGC 5 cut(s) 446, 572, 599, 624, 626
BspANI GGCC 5 cut(s) 623, 650, 677, 704, 758
BspCNI CTCAG 7 cut(s) 394, 631, 658, 685, 712, 724, 766
BspDI ATCGAT 1 cut(s) 31
BspFNI CGCG 1 cut(s) 626
BspLI GGNNCC 2 cut(s) 57, 262
BspPI GGATC 4 cut(s) 27, 36, 86, 289
BspT107I GGYRCC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 2 cut(s) 37, 279
BssECI CCNNGG 9 cut(s) 82, 506, 543, 624, 651, 678, 705, 759, 780
BssMI GATC 5 cut(s) 28, 32, 91, 294, 494
BssT1I CCWWGG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 782
Bst4CI ACNGT 4 cut(s) 231, 244, 462, 768
Bst6I CTCTTC 2 cut(s) 225, 431
BstAPI GCANNNNNTGC 2 cut(s) 140, 144
BstC8I GCNNGC 4 cut(s) 136, 145, 317, 796
BstDEI CTNAG 8 cut(s) 381, 618, 645, 672, 699, 726, 732, 753
BstDSI CCRYGG 3 cut(s) 82, 543, 624
BstF5I GGATG 2 cut(s) 489, 796
BstFNI CGCG 1 cut(s) 626
BstKTI GATC 5 cut(s) 31, 35, 94, 297, 497
BstMAI GTCTC 8 cut(s) 306, 567, 594, 621, 648, 675, 702, 756
BstMBI GATC 5 cut(s) 28, 32, 91, 294, 494
BstMWI GCNNNNNNNGC 6 cut(s) 140, 144, 260, 269, 403, 791
BstNI CCWGG 1 cut(s) 782
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 780
BstUI CGCG 1 cut(s) 626
BstV1I GCAGC 3 cut(s) 384, 556, 583
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 31
Bsu36I CCTNAGG 1 cut(s) 732
BsuRI GGCC 5 cut(s) 623, 650, 677, 704, 758
BsuTUI ATCGAT 1 cut(s) 31
BtgI CCRYGG 3 cut(s) 82, 543, 624
BtsCI GGATG 2 cut(s) 489, 796
BtsIMutI CAGTG 2 cut(s) 272, 431
Cac8I GCNNGC 4 cut(s) 136, 145, 317, 796
Cfr13I GGNCC 2 cut(s) 44, 458
Cfr42I CCGCGG 1 cut(s) 627
ClaI ATCGAT 1 cut(s) 31
CseI GACGC 1 cut(s) 821
Csp6I GTAC 2 cut(s) 240, 363
CviAII CATG 3 cut(s) 135, 235, 473
CviQI GTAC 2 cut(s) 240, 363
DdeI CTNAG 8 cut(s) 381, 618, 645, 672, 699, 726, 732, 753
DpnI GATC 5 cut(s) 30, 34, 93, 296, 496
DpnII GATC 5 cut(s) 28, 32, 91, 294, 494
DrdI GACNNNNNNGTC 4 cut(s) 586, 613, 721, 748
DseDI GACNNNNNNGTC 4 cut(s) 586, 613, 721, 748
Eam1104I CTCTTC 2 cut(s) 225, 431
EarI CTCTTC 2 cut(s) 225, 431
Eco130I CCWWGG 1 cut(s) 506
Eco47I GGWCC 2 cut(s) 44, 458
Eco57I CTGAAG 2 cut(s) 386, 455
Eco81I CCTNAGG 1 cut(s) 732
EcoRI GAATTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 780
EcoT14I CCWWGG 1 cut(s) 506
EcoT22I ATGCAT 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 506
FaeI CATG 3 cut(s) 138, 238, 476
FaqI GGGAC 1 cut(s) 763
FatI CATG 3 cut(s) 134, 234, 472
Fnu4HI GCNGC 8 cut(s) 398, 447, 570, 573, 597, 600, 624, 627
FokI GGATG 2 cut(s) 476, 803
Fsp4HI GCNGC 8 cut(s) 398, 447, 570, 573, 597, 600, 624, 627
FspBI CTAG 4 cut(s) 422, 477, 843, 849
GluI GCNGC 8 cut(s) 398, 447, 570, 573, 597, 600, 624, 627
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 2 cut(s) 42, 396
HaeIII GGCC 5 cut(s) 623, 650, 677, 704, 758
HapII CCGG 1 cut(s) 485
HgaI GACGC 1 cut(s) 821
Hin1II CATG 3 cut(s) 138, 238, 476
HincII GTYRAC 1 cut(s) 482
HindII GTYRAC 1 cut(s) 482
HinfI GANTC 4 cut(s) 196, 214, 351, 836
HpaII CCGG 1 cut(s) 485
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 363, 482
Hpy188I TCNGA 3 cut(s) 453, 494, 805
Hpy188III TCNNGA 3 cut(s) 70, 95, 370
Hpy8I GTNNAC 2 cut(s) 363, 482
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 2 cut(s) 115, 512
HpyCH4III ACNGT 4 cut(s) 231, 244, 462, 768
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 4 cut(s) 134, 138, 272, 794
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 144, 260, 269, 403, 791
HpyF3I CTNAG 8 cut(s) 381, 618, 645, 672, 699, 726, 732, 753
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 3 cut(s) 138, 238, 476
KspI CCGCGG 1 cut(s) 627
Kzo9I GATC 5 cut(s) 28, 32, 91, 294, 494
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 3 cut(s) 384, 556, 583
LweI GCATC 2 cut(s) 121, 781
MaeI CTAG 4 cut(s) 422, 477, 843, 849
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 2 cut(s) 124, 817
MalI GATC 5 cut(s) 30, 34, 93, 296, 496
MboI GATC 5 cut(s) 28, 32, 91, 294, 494
MboII GAAGA 3 cut(s) 212, 448, 489
MflI RGATCY 1 cut(s) 294
MluCI AATT 4 cut(s) 19, 87, 108, 531
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 62, 626
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 782
MvaI CCWGG 1 cut(s) 782
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 6 cut(s) 140, 144, 260, 269, 403, 791
NdeII GATC 5 cut(s) 28, 32, 91, 294, 494
NlaIII CATG 3 cut(s) 138, 238, 476
NlaIV GGNNCC 2 cut(s) 57, 262
NmeAIII GCCGAG 5 cut(s) 283, 676, 703, 730, 784
NmuCI GTSAC 2 cut(s) 124, 817
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 138
PaeI GCATGC 1 cut(s) 138
PfeI GAWTC 4 cut(s) 196, 214, 351, 836
Pfl23II CGTACG 1 cut(s) 239
PflFI GACNNNGTC 1 cut(s) 815
PkrI GCNGC 8 cut(s) 399, 448, 571, 574, 598, 601, 625, 628
PsiI TTATAA 1 cut(s) 287
Psp6I CCWGG 1 cut(s) 780
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 1 cut(s) 780
PspLI CGTACG 1 cut(s) 239
PspN4I GGNNCC 2 cut(s) 57, 262
PspPI GGNCC 2 cut(s) 44, 458
PsuI RGATCY 1 cut(s) 294
PsyI GACNNNGTC 1 cut(s) 815
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 241, 364
RsaNI GTAC 2 cut(s) 240, 363
SacII CCGCGG 1 cut(s) 627
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 8 cut(s) 398, 447, 570, 573, 597, 600, 624, 627
Sau3AI GATC 5 cut(s) 28, 32, 91, 294, 494
Sau96I GGNCC 2 cut(s) 44, 458
ScrFI CCNGG 1 cut(s) 782
SfaNI GCATC 2 cut(s) 121, 781
Sfr303I CCGCGG 1 cut(s) 627
SgrBI CCGCGG 1 cut(s) 627
SinI GGWCC 2 cut(s) 44, 458
SmlI CTYRAG 3 cut(s) 537, 564, 591
SmoI CTYRAG 3 cut(s) 537, 564, 591
SphI GCATGC 1 cut(s) 138
Sse9I AATT 4 cut(s) 19, 87, 108, 531
SsiI CCGC 5 cut(s) 446, 572, 599, 624, 626
SspMI CTAG 4 cut(s) 422, 477, 843, 849
StyD4I CCNGG 1 cut(s) 780
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 4 cut(s) 231, 244, 462, 768
TaiI ACGT 1 cut(s) 368
TaqI TCGA 2 cut(s) 31, 512
TasI AATT 4 cut(s) 19, 87, 108, 531
TauI GCSGC 5 cut(s) 449, 575, 602, 626, 629
TfiI GAWTC 4 cut(s) 196, 214, 351, 836
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 2 cut(s) 279, 438
TseFI GTSAC 2 cut(s) 124, 817
TseI GCWGC 3 cut(s) 397, 569, 596
Tsp45I GTSAC 2 cut(s) 124, 817
TspDTI ATGAA 1 cut(s) 223
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 2 cut(s) 279, 438
Tth111I GACNNNGTC 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 44, 458
XapI RAATTY 2 cut(s) 108, 531
XceI RCATGY 1 cut(s) 138
XspI CTAG 4 cut(s) 422, 477, 843, 849
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.