pycom12g16310

Chlorophyll synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010287
Physical Location & Seq
Forward (+)
18689633 .. 18693402
3770 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
ATGGCGTCCGTGCTCAATACGGTGTCGTCTATCACCTTATCGAAGCTCAACACCAACCGAGTTCGGGCTCAGTCCGCCTTCGCGCCGGTTTCCGTTTCGTTTACCAGAAGGAGGCTTACTGTAAGGGCTGCGGAGACCGATACGAACGAAGTACCGGATAAGGCACCTGCGAAAGGCGGTTCGAGCTTCAATCAGCTTCTGGGGATTAAAGGAGCTTCAAAGGAAACTAACACATGGAAGATTAGGCTTCAGCTGACAAAACCTGTAACTTGGCCTCCACTGGTATGGGGAGTAGTTTGCGGAGCTGCTGCTTCTGGAAATTTTCATTGGAGTTTCGAGGACGTTGCTAAATCAATAATGTGCATGTGCATGTCCGGCCCTTTTCTCACTGGTTATACCCAGACTCTGAATGATTGGTATGACCGAGAGATTGATGCAATTAACGAACCTTATCGTCCAATTCCCTCAGGGGCAATATCTGAGAATGAGGTTATTGCTCAAATATGGGTGCTGCTTTTAGGAGGCCTTAGCTTTGCTGGTATATTAGATGTGTGGGCAGGACATAACTTCCCAATATTATTTTACCTTGCTTTGGGTGGATCCTTGCTATCATACATTTACTCCGCTCCACCTTTAAAGCTGAAACAAAATGGATGGATTGGAAATTTTGCCCTTGGTGCAAGTTATATCAGTTTGCCATGGTGGGCTGGTCAGGCATTATTTGGGACCCTCACACCTGACATAATCGTCCTCACACTCTTGTATAGCATAGCTGGGTTGGGAATTGCTATTGTAAACGATTTTAAAAGTGTTGAAGGAGATAGAGAACTGGGACTTCAGTCACTCCCTGTAGCTTTTGGTTCTGAAACTGCCAAATGGATTTGCGTTGGTGCCATTGACATCACTCAGTTATCAATTGTTGGTTATCTGCTAGGGGCTGGTAAACCATATTATGCATTGGCTCTCCTTGCTTTGATAGGTCCTCAAGTCTTTTTCCAGTTCAAGTACTTTCTCAAAGACCCCGTCAAGTACGATGTTAAATATCAGGCCAGTGCGCAGCCGTTTCTCGTGCTCGGTCTTTTAGTAACAGCATTGGCGACAAGTCATTGA

Protein Analysis

370

Amino Acids

40.04

Weight (kDa)

8.53

Isoelectric Point (pI)

23.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 92 - 340 2.6e-39 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 175
AasI GACNNNNNNGTC 1 cut(s) 746
Acc16I TGCGCA 1 cut(s) 1056
Acc36I ACCTGC 1 cut(s) 175
AccB1I GGYRCC 2 cut(s) 163, 890
AccBSI CCGCTC 1 cut(s) 626
AccII CGCG 1 cut(s) 83
AciI CCGC 5 cut(s) 75, 131, 177, 300, 624
AclWI GGATC 2 cut(s) 594, 607
AcsI RAATTY 2 cut(s) 319, 664
AcuI CTGAAG 2 cut(s) 233, 821
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 153, 1007, 1031
AfiI CCNNNNNNNGG 4 cut(s) 64, 111, 173, 592
AgsI TTSAA 4 cut(s) 190, 219, 815, 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 310, 342
Alw21I GWGCWC 2 cut(s) 15, 1074
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 594, 607
AlwNI CAGNNNCTG 2 cut(s) 199, 406
AoxI GGCC 4 cut(s) 272, 376, 523, 1047
ApeKI GCWGC 5 cut(s) 128, 305, 308, 511, 1057
ApoI RAATTY 2 cut(s) 319, 664
ArsI GACNNNNNNTTYG 2 cut(s) 1008, 1040
AspLEI GCGC 2 cut(s) 85, 1057
AspS9I GGNCC 3 cut(s) 377, 726, 980
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 2 cut(s) 726, 980
AxyI CCTNAGG 1 cut(s) 466
BamHI GGATCC 1 cut(s) 599
BanI GGYRCC 2 cut(s) 163, 890
BanII GRGCYC 1 cut(s) 70
BarI GAAGNNNNNNTAC 2 cut(s) 100, 132
BauI CACGAG 1 cut(s) 1067
Bbv12I GWGCWC 2 cut(s) 15, 1074
BbvI GCAGC 5 cut(s) 115, 292, 295, 498, 1069
BccI CCATC 1 cut(s) 648
BceAI ACGGC 1 cut(s) 1045
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 932
BfmI CTRYAG 1 cut(s) 849
BfuAI ACCTGC 1 cut(s) 175
BisI GCNGC 5 cut(s) 129, 306, 309, 512, 1058
BlsI GCNGC 5 cut(s) 130, 307, 310, 513, 1059
BmcAI AGTACT 1 cut(s) 1007
Bme18I GGWCC 2 cut(s) 726, 980
BmgT120I GGNCC 3 cut(s) 377, 726, 980
BmiI GGNNCC 5 cut(s) 165, 601, 727, 728, 892
BmrI ACTGGG 1 cut(s) 839
BmsI GCATC 1 cut(s) 424
BmuI ACTGGG 1 cut(s) 839
BoxI GACNNNNGTC 1 cut(s) 838
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 969
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 673, 698
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 4 cut(s) 259, 289, 605, 635
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 111, 173, 592
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 5 cut(s) 285, 394, 834, 997, 1050
Bse21I CCTNAGG 1 cut(s) 466
BseDI CCNNGG 2 cut(s) 673, 698
BseGI GGATG 1 cut(s) 659
BseLI CCNNNNNNNGG 4 cut(s) 64, 111, 173, 592
BseMII CTCAG 4 cut(s) 83, 471, 480, 920
BseNI ACTGG 5 cut(s) 285, 394, 834, 997, 1050
BseXI GCAGC 5 cut(s) 115, 292, 295, 498, 1069
BseYI CCCAGC 1 cut(s) 773
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 4 cut(s) 274, 378, 525, 1049
BshNI GGYRCC 2 cut(s) 163, 890
BsiHKAI GWGCWC 2 cut(s) 15, 1074
BsiSI CCGG 3 cut(s) 86, 155, 375
BslFI GGGAC 2 cut(s) 739, 846
BslI CCNNNNNNNGG 4 cut(s) 64, 111, 173, 592
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 2 cut(s) 739, 846
BsnI GGCC 4 cut(s) 274, 378, 525, 1049
Bso31I GGTCTC 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 15, 70, 1074
Bsp143I GATC 1 cut(s) 599
Bsp19I CCATGG 1 cut(s) 698
BspACI CCGC 5 cut(s) 75, 131, 177, 300, 624
BspANI GGCC 4 cut(s) 274, 378, 525, 1049
BspCNI CTCAG 4 cut(s) 82, 472, 479, 919
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 5 cut(s) 165, 601, 727, 728, 892
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 2 cut(s) 594, 607
BspT107I GGYRCC 2 cut(s) 163, 890
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 626
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 5 cut(s) 285, 394, 834, 997, 1050
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 673, 698
BssMI GATC 1 cut(s) 599
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 2 cut(s) 673, 698
Bst2BI CACGAG 1 cut(s) 1067
Bst4CI ACNGT 2 cut(s) 22, 121
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 5 cut(s) 69, 466, 480, 527, 906
BstDSI CCRYGG 1 cut(s) 698
BstENI CCTNNNNNAGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 659
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 2 cut(s) 85, 1057
BstKTI GATC 1 cut(s) 602
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 599
BstMWI GCNNNNNNNGC 6 cut(s) 74, 183, 375, 677, 713, 968
BstNSI RCATGY 2 cut(s) 367, 373
BstPAI GACNNNNGTC 1 cut(s) 838
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 115, 292, 295, 498, 1069
BstX2I RGATCY 1 cut(s) 599
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 1 cut(s) 599
Bsu36I CCTNAGG 1 cut(s) 466
BsuRI GGCC 4 cut(s) 274, 378, 525, 1049
BtgI CCRYGG 1 cut(s) 698
BtsCI GGATG 1 cut(s) 659
BtsIMutI CAGTG 3 cut(s) 278, 387, 1057
BveI ACCTGC 1 cut(s) 175
CaiI CAGNNNCTG 2 cut(s) 199, 406
CfoI GCGC 2 cut(s) 85, 1057
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 3 cut(s) 377, 726, 980
Csp6I GTAC 3 cut(s) 152, 1006, 1030
CviAII CATG 4 cut(s) 234, 364, 370, 699
CviQI GTAC 3 cut(s) 152, 1006, 1030
DdeI CTNAG 5 cut(s) 69, 466, 480, 527, 906
DpnI GATC 1 cut(s) 601
DpnII GATC 1 cut(s) 599
DraI TTTAAA 2 cut(s) 636, 805
DrdI GACNNNNNNGTC 1 cut(s) 746
DseDI GACNNNNNNGTC 1 cut(s) 746
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 2 cut(s) 673, 698
Eco147I AGGCCT 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 726, 980
Eco57I CTGAAG 2 cut(s) 233, 821
Eco81I CCTNAGG 1 cut(s) 466
EcoNI CCTNNNNNAGG 1 cut(s) 171
EcoO109I RGGNCCY 2 cut(s) 726, 980
EcoT14I CCWWGG 2 cut(s) 673, 698
EcoT22I ATGCAT 1 cut(s) 958
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 2 cut(s) 673, 698
FaeI CATG 4 cut(s) 237, 367, 373, 702
FaqI GGGAC 2 cut(s) 739, 846
FatI CATG 4 cut(s) 233, 363, 369, 698
Fnu4HI GCNGC 5 cut(s) 129, 306, 309, 512, 1058
FokI GGATG 1 cut(s) 666
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 5 cut(s) 129, 306, 309, 512, 1058
FspBI CTAG 1 cut(s) 932
FspI TGCGCA 1 cut(s) 1056
GlaI GCGC 2 cut(s) 84, 1056
GluI GCNGC 5 cut(s) 129, 306, 309, 512, 1058
GsaI CCCAGC 1 cut(s) 777
HaeIII GGCC 4 cut(s) 274, 378, 525, 1049
HapII CCGG 3 cut(s) 86, 155, 375
HhaI GCGC 2 cut(s) 85, 1057
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 237, 367, 373, 702
Hin6I GCGC 2 cut(s) 83, 1055
HinP1I GCGC 2 cut(s) 83, 1055
HinfI GANTC 1 cut(s) 403
HpaII CCGG 3 cut(s) 86, 155, 375
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 3 cut(s) 102, 796, 944
Hpy188I TCNGA 3 cut(s) 408, 481, 865
Hpy188III TCNNGA 1 cut(s) 315
Hpy8I GTNNAC 3 cut(s) 102, 796, 944
HpyAV CCTTC 3 cut(s) 88, 102, 809
HpyCH4III ACNGT 2 cut(s) 22, 121
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 5 cut(s) 363, 369, 437, 680, 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 183, 375, 677, 713, 968
HpyF3I CTNAG 5 cut(s) 69, 466, 480, 527, 906
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 237, 367, 373, 702
HspAI GCGC 2 cut(s) 83, 1055
KflI GGGWCCC 1 cut(s) 726
Kzo9I GATC 1 cut(s) 599
LmnI GCTCC 3 cut(s) 212, 302, 631
Lsp1109I GCAGC 5 cut(s) 115, 292, 295, 498, 1069
LweI GCATC 1 cut(s) 424
MaeI CTAG 1 cut(s) 932
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 265, 840, 1084
MalI GATC 1 cut(s) 601
MbiI CCGCTC 1 cut(s) 626
MboI GATC 1 cut(s) 599
MboII GAAGA 1 cut(s) 250
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 1 cut(s) 599
MhlI GDGCHC 3 cut(s) 15, 70, 1074
MluCI AATT 6 cut(s) 319, 438, 459, 664, 783, 915
MlyI GAGTC 1 cut(s) 397
MnlI CCTC 9 cut(s) 105, 285, 331, 475, 481, 515, 740, 761, 993
Mph1103I ATGCAT 1 cut(s) 958
MseI TTAA 5 cut(s) 207, 441, 635, 804, 1038
MslI CAYNNNNRTG 2 cut(s) 283, 368
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 3 cut(s) 86, 155, 375
MunI CAATTG 1 cut(s) 915
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 6 cut(s) 74, 183, 375, 677, 713, 968
NcoI CCATGG 1 cut(s) 698
NdeII GATC 1 cut(s) 599
NlaIII CATG 4 cut(s) 237, 367, 373, 702
NlaIV GGNNCC 5 cut(s) 165, 601, 727, 728, 892
NmuCI GTSAC 1 cut(s) 840
NsbI TGCGCA 1 cut(s) 1056
NsiI ATGCAT 1 cut(s) 958
NspI RCATGY 2 cut(s) 367, 373
PaqCI CACCTGC 1 cut(s) 175
PceI AGGCCT 1 cut(s) 525
PflFI GACNNNGTC 1 cut(s) 1022
PkrI GCNGC 5 cut(s) 130, 307, 310, 513, 1059
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
PpuMI RGGWCCY 2 cut(s) 726, 980
PshAI GACNNNNGTC 1 cut(s) 838
Psp5II RGGWCCY 2 cut(s) 726, 980
PspFI CCCAGC 1 cut(s) 773
PspN4I GGNNCC 5 cut(s) 165, 601, 727, 728, 892
PspPI GGNCC 3 cut(s) 377, 726, 980
PspPPI RGGWCCY 2 cut(s) 726, 980
PstNI CAGNNNCTG 2 cut(s) 199, 406
PsuI RGATCY 1 cut(s) 599
PsyI GACNNNGTC 1 cut(s) 1022
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 3 cut(s) 153, 1007, 1031
RsaNI GTAC 3 cut(s) 152, 1006, 1030
RseI CAYNNNNRTG 2 cut(s) 283, 368
SaqAI TTAA 5 cut(s) 207, 441, 635, 804, 1038
SatI GCNGC 5 cut(s) 129, 306, 309, 512, 1058
Sau3AI GATC 1 cut(s) 599
Sau96I GGNCC 3 cut(s) 377, 726, 980
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 1 cut(s) 397
SduI GDGCHC 3 cut(s) 15, 70, 1074
SfaNI GCATC 1 cut(s) 424
SfcI CTRYAG 1 cut(s) 849
SinI GGWCC 2 cut(s) 726, 980
SmiMI CAYNNNNRTG 2 cut(s) 283, 368
SmlI CTYRAG 1 cut(s) 984
SmoI CTYRAG 1 cut(s) 984
Sse9I AATT 6 cut(s) 319, 438, 459, 664, 783, 915
SseBI AGGCCT 1 cut(s) 525
SsiI CCGC 5 cut(s) 75, 131, 177, 300, 624
SspI AATATT 1 cut(s) 576
SspMI CTAG 1 cut(s) 932
StuI AGGCCT 1 cut(s) 525
StyI CCWWGG 2 cut(s) 673, 698
TaaI ACNGT 2 cut(s) 22, 121
TaiI ACGT 1 cut(s) 345
TaqI TCGA 3 cut(s) 41, 182, 336
TaqII GACCGA 3 cut(s) 152, 438, 1064
TasI AATT 6 cut(s) 319, 438, 459, 664, 783, 915
TatI WGTACW 1 cut(s) 1005
Tru1I TTAA 5 cut(s) 207, 441, 635, 804, 1038
Tru9I TTAA 5 cut(s) 207, 441, 635, 804, 1038
TscAI CASTG 3 cut(s) 285, 394, 1057
TseFI GTSAC 1 cut(s) 840
TseI GCWGC 5 cut(s) 128, 305, 308, 511, 1057
Tsp45I GTSAC 1 cut(s) 840
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 3 cut(s) 285, 394, 1057
Tth111I GACNNNGTC 1 cut(s) 1022
VpaK11BI GGWCC 2 cut(s) 726, 980
XagI CCTNNNNNAGG 1 cut(s) 171
XapI RAATTY 2 cut(s) 319, 664
XceI RCATGY 2 cut(s) 367, 373
XspI CTAG 1 cut(s) 932
ZrmI AGTACT 1 cut(s) 1007
Zsp2I ATGCAT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.