pycom12g17580

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001663
Physical Location & Seq
Reverse (-)
19974402 .. 19975355
954 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGTGTAAGGAATCCAAGAAAAGGAGAATAGTGGCAGACCCTAGGCTAACCCCGGAGCACGAAGCTTCTGGCCTTGATGCTTTGGCCAATGCTGCAATACTCGGAGACAGTGTGGCCAATCCAGAAGCTGCATCAGTTGCTACGACAACAAAACATCCCAGGCATCGTCCTGGCTGCTCATGCATTGTGTGCATCCAGCCCCCAAGTGGAAAGGGCAAACATAAGCCTACATGCACATGCAACGTGTGCATGACCGTCAAACGCCGTTTTAAAACCATGATGATCAACAAGAAGAAACGCCAATCAGAGCGGGAAGCAGAGATTGCGTACAGAAGCCAGCACACATGGAACCCTAGGGATGAAGCAGAAGTAGACAGCACTTCTAGGCTTGTATCATCACACGTTGATCCTTCAGACAACAAGGCCAAGTCTGCAAATGAATCAGAAACCAAGAGCCAAAGCAAACTTGCTGAAACTGGGAAGGGAATACTAGACCTGAATTCCCATCCAGGCCGAGAAGGAGACTTACAAGGAGGGCCAGACCATGTGAGTATGATATCTCTTGTACAAGTAGCAACTCTTCCTCTTGAGACGTATATGAAACACAATGGTATTACCAGCTTGATATCTGAGCAACAGGAAAGTTCGACATCACATGTTCCCCCACAAGCCGCTAATGAGACTGAGGAACAACTCGATGAGAATCGCTGTCCAGAGCAGGAAAGCAGGGGTGAAGAAAGGCCGGATAAAAGCCAAGATGACCCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

257

Amino Acids

28.13

Weight (kDa)

6.38

Isoelectric Point (pI)

57.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 310
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 310, 672
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 2 cut(s) 84, 114
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 2 cut(s) 329, 567
AfiI CCNNNNNNNGG 2 cut(s) 21, 206
AflIII ACRYGT 3 cut(s) 243, 400, 655
AjnI CCWGG 3 cut(s) 158, 169, 508
AluBI AGCT 3 cut(s) 65, 128, 621
AluI AGCT 3 cut(s) 65, 128, 621
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 4 cut(s) 99, 516, 584, 674
AlwI GGATC 1 cut(s) 401
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 7 cut(s) 70, 84, 114, 423, 511, 536, 740
ApeKI GCWGC 3 cut(s) 92, 128, 174
ApoI RAATTY 1 cut(s) 499
AspA2I CCTAGG 2 cut(s) 41, 353
AspS9I GGNCC 1 cut(s) 536
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 743
AvrII CCTAGG 2 cut(s) 41, 353
BalI TGGCCA 2 cut(s) 86, 116
BarI GAAGNNNNNNTAC 4 cut(s) 510, 542, 564, 596
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 79, 115, 161
BccI CCATC 1 cut(s) 513
BceAI ACGGC 1 cut(s) 249
BciT130I CCWGG 3 cut(s) 160, 171, 510
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 4 cut(s) 99, 516, 584, 674
BfaI CTAG 4 cut(s) 42, 354, 384, 491
BisI GCNGC 4 cut(s) 93, 129, 175, 672
BlnI CCTAGG 2 cut(s) 41, 353
BlsI GCNGC 4 cut(s) 94, 130, 176, 673
Bme1390I CCNGG 4 cut(s) 53, 160, 171, 510
BmgT120I GGNCC 1 cut(s) 536
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 4 cut(s) 53, 160, 171, 510
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 4 cut(s) 67, 140, 172, 201
BmuI ACTGGG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 1 cut(s) 53
BsaBI GATNNNNATC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 41, 51, 158, 353
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 206
Bse1I ACTGG 1 cut(s) 481
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 3 cut(s) 160, 171, 510
BseDI CCNNGG 4 cut(s) 41, 51, 158, 353
BseGI GGATG 4 cut(s) 154, 192, 364, 505
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 2 cut(s) 21, 206
BseMII CTCAG 2 cut(s) 621, 675
BseNI ACTGG 1 cut(s) 481
BseXI GCAGC 3 cut(s) 79, 115, 161
BshFI GGCC 7 cut(s) 72, 86, 116, 425, 513, 538, 742
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 2 cut(s) 53, 743
BslI CCNNNNNNNGG 2 cut(s) 21, 206
BsmAI GTCTC 4 cut(s) 99, 516, 584, 674
BsmBI CGTCTC 1 cut(s) 584
BsnI GGCC 7 cut(s) 72, 86, 116, 425, 513, 538, 742
Bsp1286I GDGCHC 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 2 cut(s) 282, 406
BspACI CCGC 2 cut(s) 310, 672
BspANI GGCC 7 cut(s) 72, 86, 116, 425, 513, 538, 742
BspCNI CTCAG 2 cut(s) 622, 676
BspLI GGNNCC 1 cut(s) 350
BspPI GGATC 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 310
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 4 cut(s) 41, 51, 158, 353
BssMI GATC 2 cut(s) 282, 406
BssT1I CCWWGG 2 cut(s) 41, 353
Bst2UI CCWGG 3 cut(s) 160, 171, 510
Bst4CI ACNGT 2 cut(s) 110, 256
Bst6I CTCTTC 1 cut(s) 585
BstAPI GCANNNNNTGC 4 cut(s) 137, 189, 246, 323
BstAUI TGTACA 1 cut(s) 565
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 630, 684
BstF5I GGATG 4 cut(s) 154, 192, 364, 505
BstKTI GATC 2 cut(s) 285, 409
BstMAI GTCTC 4 cut(s) 99, 516, 584, 674
BstMBI GATC 2 cut(s) 282, 406
BstMWI GCNNNNNNNGC 7 cut(s) 92, 137, 180, 189, 246, 323, 431
BstNI CCWGG 3 cut(s) 160, 171, 510
BstNSI RCATGY 3 cut(s) 234, 240, 659
BstSCI CCNGG 4 cut(s) 51, 158, 169, 508
BstV1I GCAGC 3 cut(s) 79, 115, 161
BsuRI GGCC 7 cut(s) 72, 86, 116, 425, 513, 538, 742
BtsCI GGATG 4 cut(s) 154, 192, 364, 505
BtsIMutI CAGTG 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 338
CaiI CAGNNNCTG 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 536
Csp6I GTAC 2 cut(s) 328, 566
CviAII CATG 8 cut(s) 180, 231, 237, 250, 277, 345, 545, 656
CviQI GTAC 2 cut(s) 328, 566
DdeI CTNAG 2 cut(s) 630, 684
DpnI GATC 2 cut(s) 284, 408
DpnII GATC 2 cut(s) 282, 406
DraI TTTAAA 1 cut(s) 271
EaeI YGGCCR 2 cut(s) 84, 114
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 41, 353
Eco32I GATATC 2 cut(s) 558, 627
Eco57I CTGAAG 1 cut(s) 396
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 158, 169, 508
EcoRV GATATC 2 cut(s) 558, 627
EcoT14I CCWWGG 2 cut(s) 41, 353
EcoT22I ATGCAT 1 cut(s) 185
ErhI CCWWGG 2 cut(s) 41, 353
Esp3I CGTCTC 1 cut(s) 584
FaeI CATG 8 cut(s) 183, 234, 240, 253, 280, 348, 548, 659
FatI CATG 8 cut(s) 179, 230, 236, 249, 276, 344, 544, 655
FauI CCCGC 1 cut(s) 303
FbaI TGATCA 1 cut(s) 282
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 93, 129, 175, 672
FokI GGATG 4 cut(s) 141, 179, 371, 492
Fsp4HI GCNGC 4 cut(s) 93, 129, 175, 672
FspBI CTAG 4 cut(s) 42, 354, 384, 491
GluI GCNGC 4 cut(s) 93, 129, 175, 672
HaeIII GGCC 7 cut(s) 72, 86, 116, 425, 513, 538, 742
HapII CCGG 2 cut(s) 53, 743
Hin1II CATG 8 cut(s) 183, 234, 240, 253, 280, 348, 548, 659
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 3 cut(s) 11, 440, 703
HpaII CCGG 2 cut(s) 53, 743
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 5 cut(s) 104, 307, 415, 445, 631
Hpy188III TCNNGA 3 cut(s) 122, 587, 713
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 3 cut(s) 420, 475, 512
HpyCH4III ACNGT 2 cut(s) 110, 256
HpyCH4IV ACGT 3 cut(s) 243, 402, 593
HpyCH4V TGCA 8 cut(s) 95, 131, 183, 192, 234, 240, 249, 434
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 137, 180, 189, 246, 323, 431
HpyF3I CTNAG 2 cut(s) 630, 684
HpySE526I ACGT 3 cut(s) 243, 402, 593
Hsp92II CATG 8 cut(s) 183, 234, 240, 253, 280, 348, 548, 659
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 2 cut(s) 282, 406
LmnI GCTCC 1 cut(s) 55
Lsp1109I GCAGC 3 cut(s) 79, 115, 161
LweI GCATC 4 cut(s) 67, 140, 172, 201
MaeI CTAG 4 cut(s) 42, 354, 384, 491
MaeII ACGT 3 cut(s) 243, 402, 593
MalI GATC 2 cut(s) 284, 408
MbiI CCGCTC 1 cut(s) 310
MboI GATC 2 cut(s) 282, 406
MboII GAAGA 3 cut(s) 304, 572, 746
MhlI GDGCHC 1 cut(s) 60
MlsI TGGCCA 2 cut(s) 86, 116
MluCI AATT 1 cut(s) 499
MluNI TGGCCA 2 cut(s) 86, 116
MnlI CCTC 3 cut(s) 527, 594, 679
Mox20I TGGCCA 2 cut(s) 86, 116
Mph1103I ATGCAT 1 cut(s) 185
MscI TGGCCA 2 cut(s) 86, 116
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 235
Msp20I TGGCCA 2 cut(s) 86, 116
MspI CCGG 2 cut(s) 53, 743
MspR9I CCNGG 4 cut(s) 53, 160, 171, 510
MvaI CCWGG 3 cut(s) 160, 171, 510
MwoI GCNNNNNNNGC 7 cut(s) 92, 137, 180, 189, 246, 323, 431
NciI CCSGG 1 cut(s) 53
NdeII GATC 2 cut(s) 282, 406
NlaIII CATG 8 cut(s) 183, 234, 240, 253, 280, 348, 548, 659
NlaIV GGNNCC 1 cut(s) 350
NmeAIII GCCGAG 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 185
NspI RCATGY 3 cut(s) 234, 240, 659
PciI ACATGT 1 cut(s) 655
PfeI GAWTC 3 cut(s) 11, 440, 703
PkrI GCNGC 4 cut(s) 94, 130, 176, 673
PscI ACATGT 1 cut(s) 655
Psp6I CCWGG 3 cut(s) 158, 169, 508
PspGI CCWGG 3 cut(s) 158, 169, 508
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 1 cut(s) 536
PstNI CAGNNNCTG 1 cut(s) 128
RsaI GTAC 2 cut(s) 329, 567
RsaNI GTAC 2 cut(s) 328, 566
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 4 cut(s) 93, 129, 175, 672
Sau3AI GATC 2 cut(s) 282, 406
Sau96I GGNCC 1 cut(s) 536
ScrFI CCNGG 4 cut(s) 53, 160, 171, 510
SduI GDGCHC 1 cut(s) 60
SetI ASST 7 cut(s) 67, 130, 246, 405, 498, 596, 623
SfaNI GCATC 4 cut(s) 67, 140, 172, 201
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 1 cut(s) 499
SsiI CCGC 2 cut(s) 310, 672
SspMI CTAG 4 cut(s) 42, 354, 384, 491
StyD4I CCNGG 4 cut(s) 51, 158, 169, 508
StyI CCWWGG 2 cut(s) 41, 353
TaaI ACNGT 2 cut(s) 110, 256
TaiI ACGT 3 cut(s) 246, 405, 596
TaqI TCGA 2 cut(s) 647, 696
TasI AATT 1 cut(s) 499
TatI WGTACW 1 cut(s) 565
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 3 cut(s) 11, 440, 703
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 115
TseI GCWGC 3 cut(s) 92, 128, 174
TspDTI ATGAA 3 cut(s) 375, 453, 614
TspRI CASTG 1 cut(s) 115
XapI RAATTY 1 cut(s) 499
XceI RCATGY 3 cut(s) 234, 240, 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmaJI CCTAGG 2 cut(s) 41, 353
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 4 cut(s) 42, 354, 384, 491
Zsp2I ATGCAT 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.