Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0008791

Physical Location & Seq

Genomic Coordinates Reverse (-)

20477482 .. 20481065

Gene Structure 3584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2187 bp

ATGACTTCTATTGTTGGAGTTTCGTCTGTATATGCCCAGACTCCGTGCAGCGAGCTGTACAGAAGAGCGGCGGCGCCGGCTCCGGCAACGACGAGCAGTGCTACTCTTTCGTTGGCGTTTCCCGAGAGGCCTCACTTCAACAGTGTTTTGAGAGGCAAGAGTGGGTTGGTCAGGCAGGGCTCCGGTGTCGTTTGTTCGGCCATTGCCACTCCGAACTCGGTGCTCAGTGAAGAGGCGTTTAAGGGACTCGGTGGCTTTTCGAAGGACTCGCTTGACTCCGACTCGGAGTACGAGTCGGAAACTGAGCCGGGTTCCGCTGGTAGTGACGACGATGAGCTCGCTCTTTCCAAGCTGGGGTTGCCTCAGCGGCTTGTTGATAGCCTTGAGAGACGTGGCATTTCGAGCCTTTTTCCAATTCAGAGAGCTGTGCTAGTACCTGCACTGGAAGGTCGAGATATCATTGCTCGTGCGAAGACAGGGACTGGAAAGACATTAGCCTTTGGAATCCCAATACTCAAGCGTCTCACAGAGGAGGATGAACAGAGAGGCTCTCATAGGCGGACTGGTTATCTTCCGAGAGTTTTGGTGCTTGCCCCTACTCGGGAGTTGGCAAAGCAAGTTGAAAAGGAGATGAAAGAATCTGCCCCTTACCTAAACACTGTTTGTGTCTATGGGGGAGTTTCCTACATAACACAACAAAGTGCTCTCTCCCGTGGAGTTGATGTAGTTGTTGGAACTCCTGGTCGGATTATTGACCTGATAAACGGGAACAGTCTCAAATTGGGCGAAGTTCAATATTTGGTACTTGATGAAGCTGATTCGATGCTTGCTGTTGGATTTGAGGAGGATGTGGAAGTGATTCTACAAAAGCTTCCAGCTCAGAGGCAGAGCATGCTTTTTTCTGCAACCATGCCTGGTTGGGTGAAAAAACTATCACGGAAATATTTGGACAATCCATTAACAATTGATTTGGTTGGCGACCAAGACGAGAAGCTTGCAGAAGGGATCGATCTTTATTCTTTATCAACCACTTCAGCTTCAAAGAAGACAATTCTTAGTGATCTTATAACGGTGTATGCAAAGGGTGGGAAGACCATTGTATTTACACAAACGAAACGAGAGGCTGATGCAGTCTCAATGTCATTAACAAGTAGCATAGCTTCTGAGGCATTGCATGGAGATATATCACAGAATCAAAGAGAGAGAACATTAAATGGTTTTCGGCAAGGGAAATTCACTGTGCTTGTTGCCACTGATGTTGCATCCCGTGGACTTGATATTCCCAATGTCGATTTGGTTATCCACTACGAGCTTCCCAATGATTCAGAGACATTTGTGCATCGCTCTGGGCGTACTGGACGTGCAGGGAAACAAGGTACTGCCGTTCTGATGTTTACGAACAACCAGAGGAGAACAATTAAAACTCTTGAGCGTGATGTGGGGTGCAAGTTTGAGTTTGCTAGTCCACCAACTATGGAGGAGGTTTTGGAGTCATCTGCTCAGCACGTGGTTGCTACTCTTAGTGGAGTGCACCCTGAATCCGTACAGTTTTTCACACCAACGGCACAGAGATTGATTGATGAACAGGGAACAAGTGCTCTTGCTGCTGCACTTGCACAGCTCAGTGGATTTTCTCGTCCTCCATCATCCAAGTCCCTTATCACTCATGAGGCGGGATGGACGACATTGCAACTTATCCGAGATCGATCTTTTTCCAGAGGGTTCCTGTCTGCTAGATCTGTCACTGGGTTTCTTTCAGATGTTTATTCTACAGCTGCTGATGAAGTTGGAAAAATACATATAATTGCAGATGAACGGATTCAAGGAGCAGTTTTTATCGATCTTCCAGAGGAGATTGCCAAAGAGTTGCTGGCCAGGCAAATACCTCCCGGAAATACTATTTCCAAGATATCCAAGTTGCCAGCCCTGCAAGATGATGGGCCGGTGAGTGACAACTATGGCAGGTTTTCAGGCAGAGATCGCCGGGGAGGAGGCCGCGGAGGAGGCCGGGGTGGAGGTTTTAGGGATCGTCAAGGATCATCAGGTGGTTTTAGAAGCTCCAGGGGTTGGGGTAGTGATGGTGGTGCCGACGATTCATTTAGGAGTAGTGGCCGAAGTTATGGATCTAGCAACAGCAGACCTCGGAGTCCAAGAAGCATCGATGACGATTGGCTTATTGAACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0010501 GO:0016020 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C zf-CCHC

Protein Analysis

729

Amino Acids

78.49

Weight (kDa)

6.39

Isoelectric Point (pI)

43.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	138 - 310	6.2e-45	DEAD/DEAH box helicase
ResIII	PF04851	151 - 304	6.3e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	348 - 456	3.8e-32	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	492 - 540	9.1e-16	DDX21/DDX50 dimerisation domain
GUCT	PF08152	543 - 644	5.4e-13	GUCT (NUC152) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26742 AT5G26742 AT5G26742
fragaria_vesca	FvH4_6g08810 FvH4_6g08810 FvH4_6g08810 FvH4_6g08810
malus_domestica	MD04G1183400.v1.1 MD12G1196800.v1.1
prunus_persica	Prupe.6G301900_v2.0.a1
pyrus_communis	pycom04g16190 pycom12g18300
rosa_chinensis	RchiOBHm_Chr3g0459081
rosa_laevigata	RLG00000025121
rosa_multiflora	Rmu_sc0000466.1_g000002 Rmu_sc0003515.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420530
rosa_rugosa	Rorug03G0033400
rosa_samantha	Rh3AG091300 Rh3BG094400 Rh3CG094800 Rh3DG094800
rosa_wichuraiana	Rw3G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1067
AasI	GACNNNNNNGTC	1 cut(s)	2145
Acc36I	ACCTGC	2 cut(s)	445, 1953
AccB1I	GGYRCC	2 cut(s)	73, 2084
AccB7I	CCANNNNNTGG	1 cut(s)	2067
AccBSI	CCGCTC	1 cut(s)	68
AccII	CGCG	1 cut(s)	1998
AciI	CCGC	8 cut(s)	68, 71, 315, 367, 559, 1673, 1996, 1998
AclWI	GGATC	4 cut(s)	1013, 2034, 2044, 2131
AcoI	YGGCCR	3 cut(s)	198, 1872, 2110
AcsI	RAATTY	1 cut(s)	1232
AcuI	CTGAAG	1 cut(s)	1017
AcvI	CACGTG	1 cut(s)	1507
AcyI	GRCGYC	1 cut(s)	74
AfaI	GTAC	7 cut(s)	59, 290, 435, 804, 1354, 1378, 1545
AfiI	CCNNNNNNNGG	4 cut(s)	354, 600, 601, 2067
AgsI	TTSAA	6 cut(s)	139, 623, 794, 1041, 1823, 2180
AjiI	CACGTC	2 cut(s)	392, 1361
AjnI	CCWGG	4 cut(s)	739, 913, 1874, 2060
Alw21I	GWGCWC	5 cut(s)	225, 339, 706, 1533, 1600
Alw26I	GTCTC	5 cut(s)	382, 527, 779, 1138, 1322
Alw44I	GTGCAC	1 cut(s)	1529
AlwI	GGATC	4 cut(s)	1013, 2034, 2044, 2131
AlwNI	CAGNNNCTG	2 cut(s)	482, 1778
Ama87I	CYCGRG	2 cut(s)	122, 600
AoxI	GGCC	7 cut(s)	128, 198, 1872, 1940, 1993, 2005, 2110
ApaLI	GTGCAC	1 cut(s)	1529
ApeKI	GCWGC	4 cut(s)	48, 1604, 1607, 1775
ApoI	RAATTY	1 cut(s)	1232
Asp700I	GAANNNNTTC	2 cut(s)	858, 1818
AspLEI	GCGC	1 cut(s)	76
AspS9I	GGNCC	1 cut(s)	1940
AsuC2I	CCSGG	4 cut(s)	309, 1890, 1985, 2009
AsuHPI	GGTGA	2 cut(s)	934, 1957
AsuII	TTCGAA	1 cut(s)	260
AvaI	CYCGRG	2 cut(s)	122, 600
BaeGI	GKGCMC	1 cut(s)	1533
BaeI	ACNNNNGTAYC	2 cut(s)	1368, 1401
BalI	TGGCCA	1 cut(s)	1874
BanI	GGYRCC	2 cut(s)	73, 2084
BanII	GRGCYC	2 cut(s)	182, 339
BarI	GAAGNNNNNNTAC	4 cut(s)	846, 878, 1144, 1176
BauI	CACGAG	1 cut(s)	465
BbrPI	CACGTG	1 cut(s)	1507
BbsI	GAAGAC	3 cut(s)	479, 1052, 1097
Bbv12I	GWGCWC	5 cut(s)	225, 339, 706, 1533, 1600
BbvCI	CCTCAGC	1 cut(s)	363
BbvI	GCAGC	4 cut(s)	60, 1591, 1594, 1762
BccI	CCATC	4 cut(s)	1651, 1671, 1931, 2072
BceAI	ACGGC	2 cut(s)	1367, 1578
BcgI	CGANNNNNNTGC	4 cut(s)	202, 236, 977, 1011
BciT130I	CCWGG	4 cut(s)	741, 915, 1876, 2062
BcnI	CCSGG	4 cut(s)	309, 1890, 1985, 2009
BcoDI	GTCTC	5 cut(s)	382, 527, 779, 1138, 1322
BfaI	CTAG	4 cut(s)	431, 1461, 1734, 2127
BfmI	CTRYAG	1 cut(s)	1770
BfoI	RGCGCY	1 cut(s)	77
BfuAI	ACCTGC	2 cut(s)	445, 1953
BglI	GCCNNNNNGGC	1 cut(s)	367
BglII	AGATCT	1 cut(s)	1736
BisI	GCNGC	8 cut(s)	49, 69, 72, 368, 1605, 1608, 1776, 1996
BlpI	GCTNAGC	1 cut(s)	1500
BlsI	GCNGC	8 cut(s)	50, 70, 73, 369, 1606, 1609, 1777, 1997
Bme1390I	CCNGG	8 cut(s)	309, 741, 915, 1876, 1890, 1985, 2009, 2062
BmeT110I	CYCGRG	2 cut(s)	122, 600
BmgBI	CACGTC	2 cut(s)	392, 1361
BmgT120I	GGNCC	1 cut(s)	1940
BmiI	GGNNCC	6 cut(s)	75, 81, 181, 313, 1724, 2086
BmrFI	CCNGG	8 cut(s)	309, 741, 915, 1876, 1890, 1985, 2009, 2062
BmrI	ACTGGG	1 cut(s)	1755
BmsI	GCATC	5 cut(s)	813, 1117, 1271, 1348, 2166
BmuI	ACTGGG	1 cut(s)	1755
BpiI	GAAGAC	3 cut(s)	479, 1052, 1097
BplI	GAGNNNNNCTC	2 cut(s)	535, 567
BpmI	CTGGAG	1 cut(s)	2044
Bpu10I	CCTNAGC	1 cut(s)	363
Bpu1102I	GCTNAGC	1 cut(s)	1500
Bpu14I	TTCGAA	1 cut(s)	260
BpuEI	CTTGAG	3 cut(s)	404, 500, 1448
BpuMI	CCSGG	4 cut(s)	309, 1890, 1985, 2009
Bsa29I	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
BsaAI	YACGTR	1 cut(s)	1507
BsaHI	GRCGYC	1 cut(s)	74
BsaJI	CCNNGG	7 cut(s)	712, 1267, 1984, 1996, 2008, 2061, 2141
BsaWI	WCCGGW	1 cut(s)	182
BsaXI	ACNNNNNCTCC	4 cut(s)	278, 308, 708, 738
Bsc4I	CCNNNNNNNGG	4 cut(s)	354, 600, 601, 2067
Bse118I	RCCGGY	2 cut(s)	76, 1942
Bse1I	ACTGG	5 cut(s)	447, 487, 568, 1360, 1750
Bse3DI	GCAATG	4 cut(s)	201, 459, 1169, 1685
BseBI	CCWGG	4 cut(s)	741, 915, 1876, 2062
BseCI	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
BseDI	CCNNGG	7 cut(s)	712, 1267, 1984, 1996, 2008, 2061, 2141
BseGI	GGATG	5 cut(s)	541, 853, 1262, 1646, 1682
BseLI	CCNNNNNNNGG	4 cut(s)	354, 600, 601, 2067
BseMI	GCAATG	4 cut(s)	201, 459, 1169, 1685
BseMII	CTCAG	7 cut(s)	238, 294, 377, 893, 1155, 1514, 1636
BseNI	ACTGG	5 cut(s)	447, 487, 568, 1360, 1750
BseRI	GAGGAG	7 cut(s)	545, 857, 1423, 1493, 1865, 2004, 2016
BseSI	GKGCMC	1 cut(s)	1533
BseXI	GCAGC	4 cut(s)	60, 1591, 1594, 1762
BseYI	CCCAGC	1 cut(s)	352
BsgI	GTGCAG	4 cut(s)	67, 423, 1383, 1593
Bsh1236I	CGCG	1 cut(s)	1998
BshFI	GGCC	7 cut(s)	130, 200, 1874, 1942, 1995, 2007, 2112
BshNI	GGYRCC	2 cut(s)	73, 2084
BshVI	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
BsiHKAI	GWGCWC	5 cut(s)	225, 339, 706, 1533, 1600
BsiHKCI	CYCGRG	2 cut(s)	122, 600
BsiSI	CCGG	8 cut(s)	77, 83, 183, 308, 1890, 1943, 1984, 2008
BslFI	GGGAC	3 cut(s)	258, 493, 1639
BslI	CCNNNNNNNGG	4 cut(s)	354, 600, 601, 2067
BsmAI	GTCTC	5 cut(s)	382, 527, 779, 1138, 1322
BsmBI	CGTCTC	2 cut(s)	382, 527
BsmFI	GGGAC	3 cut(s)	258, 493, 1639
BsnI	GGCC	7 cut(s)	130, 200, 1874, 1942, 1995, 2007, 2112
BsoBI	CYCGRG	2 cut(s)	122, 600
Bsp119I	TTCGAA	1 cut(s)	260
Bsp1286I	GDGCHC	6 cut(s)	182, 225, 339, 706, 1533, 1600
Bsp1407I	TGTACA	1 cut(s)	57
Bsp1720I	GCTNAGC	1 cut(s)	1500
BspACI	CCGC	8 cut(s)	68, 71, 315, 367, 559, 1673, 1996, 1998
BspANI	GGCC	7 cut(s)	130, 200, 1874, 1942, 1995, 2007, 2112
BspCNI	CTCAG	7 cut(s)	237, 295, 376, 892, 1156, 1513, 1635
BspDI	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
BspFNI	CGCG	1 cut(s)	1998
BspHI	TCATGA	1 cut(s)	1666
BspLI	GGNNCC	6 cut(s)	75, 81, 181, 313, 1724, 2086
BspMI	ACCTGC	2 cut(s)	445, 1953
BspPI	GGATC	4 cut(s)	1013, 2034, 2044, 2131
BspQI	GCTCTTC	1 cut(s)	58
BspT104I	TTCGAA	1 cut(s)	260
BspT107I	GGYRCC	2 cut(s)	73, 2084
BsrBI	CCGCTC	1 cut(s)	68
BsrDI	GCAATG	4 cut(s)	201, 459, 1169, 1685
BsrFI	RCCGGY	2 cut(s)	76, 1942
BsrGI	TGTACA	1 cut(s)	57
BsrI	ACTGG	5 cut(s)	447, 487, 568, 1360, 1750
BssAI	RCCGGY	2 cut(s)	76, 1942
BssECI	CCNNGG	7 cut(s)	712, 1267, 1984, 1996, 2008, 2061, 2141
BssNI	GRCGYC	1 cut(s)	74
BssSI	CACGAG	1 cut(s)	465
Bst2BI	CACGAG	1 cut(s)	465
Bst2UI	CCWGG	4 cut(s)	741, 915, 1876, 2062
Bst4CI	ACNGT	7 cut(s)	143, 661, 773, 1072, 1240, 1548, 2184
Bst6I	CTCTTC	2 cut(s)	58, 225
BstACI	GRCGYC	1 cut(s)	74
BstAPI	GCANNNNNTGC	1 cut(s)	892
BstAUI	TGTACA	1 cut(s)	57
BstBAI	YACGTR	1 cut(s)	1507
BstBI	TTCGAA	1 cut(s)	260
BstC8I	GCNNGC	9 cut(s)	53, 78, 339, 591, 828, 893, 996, 1872, 1923
BstDEI	CTNAG	9 cut(s)	224, 303, 363, 879, 1055, 1164, 1500, 1520, 1622
BstDSI	CCRYGG	3 cut(s)	712, 1267, 1996
BstF5I	GGATG	5 cut(s)	541, 853, 1262, 1646, 1682
BstFNI	CGCG	1 cut(s)	1998
BstH2I	RGCGCY	1 cut(s)	77
BstHHI	GCGC	1 cut(s)	76
BstMAI	GTCTC	5 cut(s)	382, 527, 779, 1138, 1322
BstNI	CCWGG	4 cut(s)	741, 915, 1876, 2062
BstNSI	RCATGY	1 cut(s)	895
BstSCI	CCNGG	8 cut(s)	307, 739, 913, 1874, 1888, 1983, 2007, 2060
BstSFI	CTRYAG	1 cut(s)	1770
BstSLI	GKGCMC	1 cut(s)	1533
BstUI	CGCG	1 cut(s)	1998
BstV1I	GCAGC	4 cut(s)	60, 1591, 1594, 1762
BstV2I	GAAGAC	3 cut(s)	479, 1052, 1097
BstX2I	RGATCY	2 cut(s)	1736, 2123
BstYI	RGATCY	2 cut(s)	1736, 2123
Bsu15I	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
BsuRI	GGCC	7 cut(s)	130, 200, 1874, 1942, 1995, 2007, 2112
BsuTUI	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
BtgI	CCRYGG	3 cut(s)	712, 1267, 1996
BtgZI	GCGATG	1 cut(s)	1325
BtrI	CACGTC	2 cut(s)	392, 1361
BtsCI	GGATG	5 cut(s)	541, 853, 1262, 1646, 1682
BtsI	GCAGTG	1 cut(s)	103
BtsIMutI	CAGTG	9 cut(s)	103, 148, 232, 440, 657, 1236, 1251, 1630, 1743
BveI	ACCTGC	2 cut(s)	445, 1953
Cac8I	GCNNGC	9 cut(s)	53, 78, 339, 591, 828, 893, 996, 1872, 1923
CaiI	CAGNNNCTG	2 cut(s)	482, 1778
CciI	TCATGA	1 cut(s)	1666
CfoI	GCGC	1 cut(s)	76
Cfr10I	RCCGGY	2 cut(s)	76, 1942
Cfr13I	GGNCC	1 cut(s)	1940
Cfr42I	CCGCGG	1 cut(s)	1999
ClaI	ATCGAT	4 cut(s)	1008, 1705, 1839, 2160
CseI	GACGC	1 cut(s)	509
Csp6I	GTAC	7 cut(s)	58, 289, 434, 803, 1353, 1377, 1544
CspCI	CAANNNNNGTGG	2 cut(s)	1240, 1275
CviAII	CATG	4 cut(s)	892, 910, 1175, 1667
CviQI	GTAC	7 cut(s)	58, 289, 434, 803, 1353, 1377, 1544
DdeI	CTNAG	9 cut(s)	224, 303, 363, 879, 1055, 1164, 1500, 1520, 1622
DinI	GGCGCC	1 cut(s)	75
DrdI	GACNNNNNNGTC	1 cut(s)	2145
DseDI	GACNNNNNNGTC	1 cut(s)	2145
EaeI	YGGCCR	3 cut(s)	198, 1872, 2110
Eam1104I	CTCTTC	2 cut(s)	58, 225
EarI	CTCTTC	2 cut(s)	58, 225
EciI	GGCGGA	1 cut(s)	574
Ecl136II	GAGCTC	1 cut(s)	337
Eco147I	AGGCCT	1 cut(s)	130
Eco24I	GRGCYC	2 cut(s)	182, 339
Eco32I	GATATC	2 cut(s)	457, 1911
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	1 cut(s)	1017
Eco72I	CACGTG	1 cut(s)	1507
Eco88I	CYCGRG	2 cut(s)	122, 600
EcoICRI	GAGCTC	1 cut(s)	337
EcoRII	CCWGG	4 cut(s)	739, 913, 1874, 2060
EcoRV	GATATC	2 cut(s)	457, 1911
EcoT38I	GRGCYC	2 cut(s)	182, 339
EgeI	GGCGCC	1 cut(s)	75
EheI	GGCGCC	1 cut(s)	75
Esp3I	CGTCTC	2 cut(s)	382, 527
FaeI	CATG	4 cut(s)	895, 913, 1178, 1670
FaqI	GGGAC	3 cut(s)	258, 493, 1639
FatI	CATG	4 cut(s)	891, 909, 1174, 1666
FauI	CCCGC	1 cut(s)	1666
Fnu4HI	GCNGC	8 cut(s)	49, 69, 72, 368, 1605, 1608, 1776, 1996
FokI	GGATG	5 cut(s)	548, 860, 1249, 1633, 1689
FriOI	GRGCYC	2 cut(s)	182, 339
Fsp4HI	GCNGC	8 cut(s)	49, 69, 72, 368, 1605, 1608, 1776, 1996
FspBI	CTAG	4 cut(s)	431, 1461, 1734, 2127
GlaI	GCGC	1 cut(s)	75
GluI	GCNGC	8 cut(s)	49, 69, 72, 368, 1605, 1608, 1776, 1996
GsaI	CCCAGC	1 cut(s)	356
GsuI	CTGGAG	1 cut(s)	2044
HaeII	RGCGCY	1 cut(s)	77
HaeIII	GGCC	7 cut(s)	130, 200, 1874, 1942, 1995, 2007, 2112
HapII	CCGG	8 cut(s)	77, 83, 183, 308, 1890, 1943, 1984, 2008
HgaI	GACGC	1 cut(s)	509
HhaI	GCGC	1 cut(s)	76
Hin1I	GRCGYC	1 cut(s)	74
Hin1II	CATG	4 cut(s)	895, 913, 1178, 1670
Hin6I	GCGC	1 cut(s)	74
HinP1I	GCGC	1 cut(s)	74
HindIII	AAGCTT	2 cut(s)	869, 992
HpaII	CCGG	8 cut(s)	77, 83, 183, 308, 1890, 1943, 1984, 2008
HphI	GGTGA	2 cut(s)	934, 1957
Hpy166II	GTNNAC	4 cut(s)	1271, 1395, 1466, 1531
Hpy188III	TCNNGA	7 cut(s)	122, 452, 602, 1427, 1667, 1716, 1847
Hpy8I	GTNNAC	4 cut(s)	1271, 1395, 1466, 1531
Hpy99I	CGWCG	3 cut(s)	94, 332, 2093
HpyAV	CCTTC	3 cut(s)	256, 440, 995
HpyCH4III	ACNGT	7 cut(s)	143, 661, 773, 1072, 1240, 1548, 2184
HpyCH4IV	ACGT	3 cut(s)	391, 1360, 1506
HpyF3I	CTNAG	9 cut(s)	224, 303, 363, 879, 1055, 1164, 1500, 1520, 1622
HpySE526I	ACGT	3 cut(s)	391, 1360, 1506
Hsp92I	GRCGYC	1 cut(s)	74
Hsp92II	CATG	4 cut(s)	895, 913, 1178, 1670
HspAI	GCGC	1 cut(s)	74
KasI	GGCGCC	1 cut(s)	73
KroI	GCCGGC	1 cut(s)	76
KroNI	GCCGGC	1 cut(s)	78
KspI	CCGCGG	1 cut(s)	1999
LguI	GCTCTTC	1 cut(s)	58
LmnI	GCTCC	4 cut(s)	85, 185, 1826, 2063
Lsp1109I	GCAGC	4 cut(s)	60, 1591, 1594, 1762
LweI	GCATC	5 cut(s)	813, 1117, 1271, 1348, 2166
MaeI	CTAG	4 cut(s)	431, 1461, 1734, 2127
MaeII	ACGT	3 cut(s)	391, 1360, 1506
MaeIII	GTNAC	3 cut(s)	323, 1741, 1949
MbiI	CCGCTC	1 cut(s)	68
MboII	GAAGA	7 cut(s)	75, 242, 484, 563, 1057, 1102, 1835
MfeI	CAATTG	1 cut(s)	963
MflI	RGATCY	2 cut(s)	1736, 2123
MhlI	GDGCHC	6 cut(s)	182, 225, 339, 706, 1533, 1600
MlsI	TGGCCA	1 cut(s)	1874
MluCI	AATT	7 cut(s)	414, 779, 963, 1050, 1232, 1416, 1803
MluNI	TGGCCA	1 cut(s)	1874
Mly113I	GGCGCC	1 cut(s)	74
MlyI	GAGTC	8 cut(s)	34, 240, 260, 269, 275, 302, 1499, 2155
MmeI	TCCRAC	6 cut(s)	276, 303, 712, 725, 814, 1768
Mox20I	TGGCCA	1 cut(s)	1874
MroNI	GCCGGC	1 cut(s)	76
MroXI	GAANNNNTTC	2 cut(s)	858, 1818
MscI	TGGCCA	1 cut(s)	1874
MseI	TTAA	5 cut(s)	240, 959, 1145, 1211, 1419
Msp20I	TGGCCA	1 cut(s)	1874
MspA1I	CMGCKG	4 cut(s)	317, 367, 1775, 1998
MspI	CCGG	8 cut(s)	77, 83, 183, 308, 1890, 1943, 1984, 2008
MspR9I	CCNGG	8 cut(s)	309, 741, 915, 1876, 1890, 1985, 2009, 2062
MunI	CAATTG	1 cut(s)	963
MvaI	CCWGG	4 cut(s)	741, 915, 1876, 2062
MvnI	CGCG	1 cut(s)	1998
NaeI	GCCGGC	1 cut(s)	78
NarI	GGCGCC	1 cut(s)	74
NciI	CCSGG	4 cut(s)	309, 1890, 1985, 2009
NgoMIV	GCCGGC	1 cut(s)	76
NlaIII	CATG	4 cut(s)	895, 913, 1178, 1670
NlaIV	GGNNCC	6 cut(s)	75, 81, 181, 313, 1724, 2086
NmuCI	GTSAC	3 cut(s)	323, 1741, 1949
NspI	RCATGY	1 cut(s)	895
NspV	TTCGAA	1 cut(s)	260
PaeI	GCATGC	1 cut(s)	895
PagI	TCATGA	1 cut(s)	1666
PceI	AGGCCT	1 cut(s)	130
PciSI	GCTCTTC	1 cut(s)	58
PcsI	WCGNNNNNNNCGW	1 cut(s)	1348
PdiI	GCCGGC	1 cut(s)	78
PdmI	GAANNNNTTC	2 cut(s)	858, 1818
PfeI	GAWTC	9 cut(s)	504, 638, 818, 859, 1192, 1322, 1538, 1819, 2093
PflMI	CCANNNNNTGG	1 cut(s)	2067
PfoI	TCCNGGA	1 cut(s)	1888
PkrI	GCNGC	8 cut(s)	50, 70, 73, 369, 1606, 1609, 1777, 1997
PleI	GAGTC	8 cut(s)	34, 240, 260, 269, 275, 301, 1498, 2154
PluTI	GGCGCC	1 cut(s)	77
PmaCI	CACGTG	1 cut(s)	1507
PmlI	CACGTG	1 cut(s)	1507
PpsI	GAGTC	8 cut(s)	34, 240, 260, 269, 275, 301, 1498, 2154
Ppu21I	YACGTR	1 cut(s)	1507
PsiI	TTATAA	1 cut(s)	1067
Psp124BI	GAGCTC	1 cut(s)	339
Psp6I	CCWGG	4 cut(s)	739, 913, 1874, 2060
PspCI	CACGTG	1 cut(s)	1507
PspFI	CCCAGC	1 cut(s)	352
PspGI	CCWGG	4 cut(s)	739, 913, 1874, 2060
PspN4I	GGNNCC	6 cut(s)	75, 81, 181, 313, 1724, 2086
PspPI	GGNCC	1 cut(s)	1940
PstNI	CAGNNNCTG	2 cut(s)	482, 1778
PsuI	RGATCY	2 cut(s)	1736, 2123
PvuII	CAGCTG	1 cut(s)	1775
RsaI	GTAC	7 cut(s)	59, 290, 435, 804, 1354, 1378, 1545
RsaNI	GTAC	7 cut(s)	58, 289, 434, 803, 1353, 1377, 1544
SacI	GAGCTC	1 cut(s)	339
SacII	CCGCGG	1 cut(s)	1999
SapI	GCTCTTC	1 cut(s)	58
SaqAI	TTAA	5 cut(s)	240, 959, 1145, 1211, 1419
SatI	GCNGC	8 cut(s)	49, 69, 72, 368, 1605, 1608, 1776, 1996
Sau96I	GGNCC	1 cut(s)	1940
SchI	GAGTC	8 cut(s)	34, 240, 260, 269, 275, 302, 1499, 2155
ScrFI	CCNGG	8 cut(s)	309, 741, 915, 1876, 1890, 1985, 2009, 2062
SduI	GDGCHC	6 cut(s)	182, 225, 339, 706, 1533, 1600
SfaNI	GCATC	5 cut(s)	813, 1117, 1271, 1348, 2166
SfcI	CTRYAG	1 cut(s)	1770
SfoI	GGCGCC	1 cut(s)	75
Sfr303I	CCGCGG	1 cut(s)	1999
SfuI	TTCGAA	1 cut(s)	260
SgrBI	CCGCGG	1 cut(s)	1999
SmlI	CTYRAG	3 cut(s)	383, 515, 1427
SmoI	CTYRAG	3 cut(s)	383, 515, 1427
SphI	GCATGC	1 cut(s)	895
Sse9I	AATT	7 cut(s)	414, 779, 963, 1050, 1232, 1416, 1803
SseBI	AGGCCT	1 cut(s)	130
SsiI	CCGC	8 cut(s)	68, 71, 315, 367, 559, 1673, 1996, 1998
SspDI	GGCGCC	1 cut(s)	73
SspI	AATATT	2 cut(s)	797, 944
SspMI	CTAG	4 cut(s)	431, 1461, 1734, 2127
SstI	GAGCTC	1 cut(s)	339
StuI	AGGCCT	1 cut(s)	130
StyD4I	CCNGG	8 cut(s)	307, 739, 913, 1874, 1888, 1983, 2007, 2060
TaaI	ACNGT	7 cut(s)	143, 661, 773, 1072, 1240, 1548, 2184
TaiI	ACGT	3 cut(s)	394, 1363, 1509
TaqI	TCGA	9 cut(s)	260, 401, 451, 821, 1008, 1290, 1705, 1839, 2160
TasI	AATT	7 cut(s)	414, 779, 963, 1050, 1232, 1416, 1803
TatI	WGTACW	1 cut(s)	57
TauI	GCSGC	4 cut(s)	71, 74, 370, 1998
TfiI	GAWTC	9 cut(s)	504, 638, 818, 859, 1192, 1322, 1538, 1819, 2093
Tru1I	TTAA	5 cut(s)	240, 959, 1145, 1211, 1419
Tru9I	TTAA	5 cut(s)	240, 959, 1145, 1211, 1419
TscAI	CASTG	9 cut(s)	103, 148, 232, 447, 664, 1243, 1258, 1630, 1750
TseFI	GTSAC	3 cut(s)	323, 1741, 1949
TseI	GCWGC	4 cut(s)	48, 1604, 1607, 1775
Tsp45I	GTSAC	3 cut(s)	323, 1741, 1949
TspDTI	ATGAA	7 cut(s)	552, 647, 825, 1596, 1797, 1827, 2085
TspGWI	ACGGA	4 cut(s)	33, 952, 1531, 1831
TspRI	CASTG	9 cut(s)	103, 148, 232, 447, 664, 1243, 1258, 1630, 1750
Van91I	CCANNNNNTGG	1 cut(s)	2067
VneI	GTGCAC	1 cut(s)	1529
XapI	RAATTY	1 cut(s)	1232
XceI	RCATGY	1 cut(s)	895
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1291, 1867
XmnI	GAANNNNTTC	2 cut(s)	858, 1818
XspI	CTAG	4 cut(s)	431, 1461, 1734, 2127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.