pycom12g19030

Chloroplast stem-loop binding protein of 41 kDa b

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008555
Physical Location & Seq
Reverse (-)
21020298 .. 21022787
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGGCAAGGTTGGTGGCTGTGCAAACACAGCAGAGAAACCCATCTCTCTCTCTCCTCCCTCCTTCCTCTCTCTCTGACTTCAATGGCACCAAACTCCTCCACTCCCAACTCCAGTGTAAAAGAAGGGCATCGCAGCCAAGAGGAGGGGCATTGCAAGTTTCAGCATCGAGTGCAAAGAAGATTCTTATAATGGGAGGAACTCGATTCATCGGCGTCTTCTTGTCAAGACTCCTTGTTAAAGACGGTCATCAGGTGACTCTGTTTACCAGAGGAAAAGCACCCATTACTCAGCAGTTGCCAGGAGAATCTGATGCGGATTACACAGATTTCGCTTCAAAGATTTTGCACTTGAAAGGGGACAGAAAGGACTATGACTTTGTGAAATCCAGTCTTTCAGCTGAAGGCTATGATGTTGTTTACGATATAAATGGTGCGCATAGACGAGAGGCAGAAGAAGTTGTGCCGATAATTGAGGGACTACCGAAGTTAGAACAGTACATATACTGCTCTTCAGCCGGTGTCTATCTCAAATCTGATCAGCTACCTCACTTTGAGATCGATGCAGTTGATCCAAAGAGCAGGCACAAGGGAAAGCTCGAGACAGAGAGCTTGCTCAAATCAAAGGGTGTGAACTGGACTTCAATAAGGCCAGTCTACATCTATGGACCATTGAACTATAACCCTGTTGAAGAGTGGTTCTTCCACCGGTTGAAAGCTGGCCGCCCAATCCCAGTTCCAAACTCAGGGATACAAATTACACAGCTTGGTCATGTCAAGGACTTAGCGACAGCCTTCATTAAGGTTCTTGGTAACGAGAAGGCCAGCAAACAAGTATTCAACATCTCGGGAGACAAATATGTCACCTTTGATGGACTAGCAAAAGCATGCGCAAAGGCTGCTGGTTTTCCTGAGCCTGAGATCGATTCATTACAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000427 GO:0000428 GO:0001101 GO:0001871 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005777 GO:0005840 GO:0005911 GO:0005975 GO:0005996 GO:0006139 GO:0006355 GO:0006364 GO:0006396 GO:0006412 GO:0006417 GO:0006518 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007623 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009506 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009657 GO:0009658 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0009991 GO:0010035 GO:0010287 GO:0010297 GO:0010319 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016020 GO:0016043 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0019538 GO:0022613 GO:0030054 GO:0030246 GO:0030247 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031668 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0032544 GO:0032991 GO:0033554 GO:0034248 GO:0034250 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042254 GO:0042579 GO:0042631 GO:0042742 GO:0043043 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0045893 GO:0045935 GO:0046483 GO:0048046 GO:0048511 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:1990904 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

313

Amino Acids

34.44

Weight (kDa)

9.22

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 61 - 281 1.3e-16 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 65 - 220 3.3e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AasI GACNNNNNNGTC 1 cut(s) 857
Acc16I TGCGCA 2 cut(s) 435, 889
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 654
AciI CCGC 2 cut(s) 314, 721
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 718
AcuI CTGAAG 2 cut(s) 420, 495
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 2 cut(s) 143, 743
AgeI ACCGGT 1 cut(s) 705
AgsI TTSAA 8 cut(s) 82, 336, 352, 642, 673, 689, 712, 838
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 6 cut(s) 398, 541, 595, 609, 716, 763
AluI AGCT 6 cut(s) 398, 541, 595, 609, 716, 763
Alw26I GTCTC 2 cut(s) 593, 843
AlwI GGATC 1 cut(s) 563
Ama87I CYCGRG 2 cut(s) 596, 844
AoxI GGCC 3 cut(s) 647, 718, 819
ApeKI GCWGC 2 cut(s) 133, 896
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AsiGI ACCGGT 1 cut(s) 705
AspLEI GCGC 2 cut(s) 436, 890
AspS9I GGNCC 1 cut(s) 665
AsuHPI GGTGA 2 cut(s) 265, 853
AvaI CYCGRG 2 cut(s) 596, 844
AvaII GGWCC 1 cut(s) 665
BanI GGYRCC 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 208
BbvI GCAGC 2 cut(s) 145, 883
BccI CCATC 2 cut(s) 49, 863
BciT130I CCWGG 1 cut(s) 300
BciVI GTATCC 1 cut(s) 741
BclI TGATCA 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 593, 843
BfaI CTAG 1 cut(s) 875
BfuI GTATCC 1 cut(s) 741
BisI GCNGC 3 cut(s) 134, 721, 897
BlsI GCNGC 3 cut(s) 135, 722, 898
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 2 cut(s) 596, 844
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 725
BmsI GCATC 4 cut(s) 137, 173, 301, 550
BmuI ACTGGG 1 cut(s) 725
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 909
Bsa29I ATCGAT 2 cut(s) 558, 921
BsaHI GRCGYC 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 705
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 743
Bse118I RCCGGY 2 cut(s) 515, 705
Bse1I ACTGG 5 cut(s) 112, 387, 638, 650, 731
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 300
BseCI ATCGAT 2 cut(s) 558, 921
BseLI CCNNNNNNNGG 2 cut(s) 143, 743
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 4 cut(s) 302, 756, 900, 906
BseNI ACTGG 5 cut(s) 112, 387, 638, 650, 731
BseRI GAGGAG 3 cut(s) 44, 86, 156
BseXI GCAGC 2 cut(s) 145, 883
BshFI GGCC 3 cut(s) 649, 720, 821
BshNI GGYRCC 1 cut(s) 86
BshTI ACCGGT 1 cut(s) 705
BshVI ATCGAT 2 cut(s) 558, 921
BsiHKCI CYCGRG 2 cut(s) 596, 844
BsiSI CCGG 2 cut(s) 516, 706
BslFI GGGAC 2 cut(s) 371, 489
BslI CCNNNNNNNGG 2 cut(s) 143, 743
BsmAI GTCTC 2 cut(s) 593, 843
BsmFI GGGAC 2 cut(s) 371, 489
BsnI GGCC 3 cut(s) 649, 720, 821
BsoBI CYCGRG 2 cut(s) 596, 844
Bsp143I GATC 4 cut(s) 535, 555, 568, 918
BspACI CCGC 2 cut(s) 314, 721
BspANI GGCC 3 cut(s) 649, 720, 821
BspCNI CTCAG 4 cut(s) 301, 755, 901, 907
BspDI ATCGAT 2 cut(s) 558, 921
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 149
BsrFI RCCGGY 2 cut(s) 515, 705
BsrI ACTGG 5 cut(s) 112, 387, 638, 650, 731
BssAI RCCGGY 2 cut(s) 515, 705
BssMI GATC 4 cut(s) 535, 555, 568, 918
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 245, 495
Bst6I CTCTTC 2 cut(s) 514, 684
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 2 cut(s) 170, 896
BstC8I GCNNGC 5 cut(s) 581, 611, 718, 823, 886
BstDEI CTNAG 5 cut(s) 288, 742, 781, 909, 915
BstHHI GCGC 2 cut(s) 436, 890
BstKTI GATC 4 cut(s) 538, 558, 571, 921
BstMAI GTCTC 2 cut(s) 593, 843
BstMBI GATC 4 cut(s) 535, 555, 568, 918
BstMWI GCNNNNNNNGC 3 cut(s) 28, 170, 896
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 1 cut(s) 888
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 145, 883
BstV2I GAAGAC 1 cut(s) 208
Bsu15I ATCGAT 2 cut(s) 558, 921
BsuI GTATCC 1 cut(s) 741
BsuRI GGCC 3 cut(s) 649, 720, 821
BsuTUI ATCGAT 2 cut(s) 558, 921
BtgZI GCGATG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 119
Cac8I GCNNGC 5 cut(s) 581, 611, 718, 823, 886
CfoI GCGC 2 cut(s) 436, 890
Cfr10I RCCGGY 2 cut(s) 515, 705
Cfr13I GGNCC 1 cut(s) 665
ClaI ATCGAT 2 cut(s) 558, 921
CseI GACGC 1 cut(s) 202
Csp6I GTAC 1 cut(s) 496
CspAI ACCGGT 1 cut(s) 705
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 2 cut(s) 770, 885
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 5 cut(s) 288, 742, 781, 909, 915
DpnI GATC 4 cut(s) 537, 557, 570, 920
DpnII GATC 4 cut(s) 535, 555, 568, 918
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 514, 684
EarI CTCTTC 2 cut(s) 514, 684
Eco47I GGWCC 1 cut(s) 665
Eco57I CTGAAG 2 cut(s) 420, 495
Eco88I CYCGRG 2 cut(s) 596, 844
EcoRII CCWGG 1 cut(s) 298
FaeI CATG 2 cut(s) 773, 888
FaqI GGGAC 2 cut(s) 371, 489
FatI CATG 2 cut(s) 769, 884
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 3 cut(s) 134, 721, 897
Fsp4HI GCNGC 3 cut(s) 134, 721, 897
FspAI RTGCGCAY 1 cut(s) 435
FspBI CTAG 1 cut(s) 875
FspI TGCGCA 2 cut(s) 435, 889
GlaI GCGC 2 cut(s) 435, 889
GluI GCNGC 3 cut(s) 134, 721, 897
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 3 cut(s) 649, 720, 821
HapII CCGG 2 cut(s) 516, 706
HgaI GACGC 1 cut(s) 202
HhaI GCGC 2 cut(s) 436, 890
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 2 cut(s) 773, 888
Hin6I GCGC 2 cut(s) 434, 888
HinP1I GCGC 2 cut(s) 434, 888
HinfI GANTC 6 cut(s) 181, 204, 228, 256, 305, 923
HpaII CCGG 2 cut(s) 516, 706
HphI GGTGA 2 cut(s) 265, 853
Hpy166II GTNNAC 4 cut(s) 264, 418, 631, 655
Hpy188I TCNGA 3 cut(s) 76, 310, 535
Hpy188III TCNNGA 4 cut(s) 225, 598, 846, 908
Hpy8I GTNNAC 4 cut(s) 264, 418, 631, 655
HpyAV CCTTC 5 cut(s) 72, 117, 395, 802, 811
HpyCH4III ACNGT 2 cut(s) 245, 495
HpyCH4V TGCA 5 cut(s) 22, 154, 173, 346, 563
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 170, 896
HpyF3I CTNAG 5 cut(s) 288, 742, 781, 909, 915
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 2 cut(s) 773, 888
HspAI GCGC 2 cut(s) 434, 888
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 4 cut(s) 535, 555, 568, 918
LguI GCTCTTC 1 cut(s) 514
Lsp1109I GCAGC 2 cut(s) 145, 883
LweI GCATC 4 cut(s) 137, 173, 301, 550
MaeI CTAG 1 cut(s) 875
MaeIII GTNAC 3 cut(s) 253, 809, 859
MalI GATC 4 cut(s) 537, 557, 570, 920
MboI GATC 4 cut(s) 535, 555, 568, 918
MboII GAAGA 6 cut(s) 190, 208, 464, 501, 691, 701
MluCI AATT 2 cut(s) 468, 753
MlyI GAGTC 2 cut(s) 222, 250
MseI TTAA 2 cut(s) 237, 798
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 2 cut(s) 516, 706
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 3 cut(s) 28, 170, 896
NdeII GATC 4 cut(s) 535, 555, 568, 918
NlaIII CATG 2 cut(s) 773, 888
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 253, 859
NsbI TGCGCA 2 cut(s) 435, 889
NspI RCATGY 1 cut(s) 888
PaeI GCATGC 1 cut(s) 888
PaeR7I CTCGAG 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 514
PfeI GAWTC 4 cut(s) 181, 204, 305, 923
PinAI ACCGGT 1 cut(s) 705
PkrI GCNGC 3 cut(s) 135, 722, 898
PleI GAGTC 2 cut(s) 222, 250
PpsI GAGTC 2 cut(s) 222, 250
PsiI TTATAA 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 665
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SapI GCTCTTC 1 cut(s) 514
SaqAI TTAA 2 cut(s) 237, 798
SatI GCNGC 3 cut(s) 134, 721, 897
Sau3AI GATC 4 cut(s) 535, 555, 568, 918
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 2 cut(s) 222, 250
ScrFI CCNGG 1 cut(s) 300
SfaNI GCATC 4 cut(s) 137, 173, 301, 550
Sfr274I CTCGAG 1 cut(s) 596
SinI GGWCC 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
SphI GCATGC 1 cut(s) 888
Sse9I AATT 2 cut(s) 468, 753
SsiI CCGC 2 cut(s) 314, 721
SspMI CTAG 1 cut(s) 875
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 245, 495
TaqI TCGA 5 cut(s) 167, 202, 558, 597, 921
TasI AATT 2 cut(s) 468, 753
TatI WGTACW 1 cut(s) 495
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 4 cut(s) 181, 204, 305, 923
Tru1I TTAA 2 cut(s) 237, 798
Tru9I TTAA 2 cut(s) 237, 798
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 2 cut(s) 253, 859
TseI GCWGC 2 cut(s) 133, 896
Tsp45I GTSAC 2 cut(s) 253, 859
TspDTI ATGAA 3 cut(s) 196, 784, 915
TspRI CASTG 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 665
XceI RCATGY 1 cut(s) 888
XhoI CTCGAG 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.