pycom12g19270

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013694
Physical Location & Seq
Reverse (-)
21170006 .. 21177843
7838 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3006 bp
ATGTTACCGAATTTTGAAAATACTCTTGTGGAGAAGAAGGTGGCGAAAGAGAATTGGCAACCAACATTGATAGGTTCTACATCTGCAGAACAGAAGCGGTTCCCCCAGCTGCACTCACAAAAGAAAGAGATGAAAAGACGTCGTGGTTTTCCACGAGGGACACAAAGTGCTTATACTATCTTCCTCAGGAAAGAATGTGAGCGGTTGAAAAATTCTGCTTCAGAGCAACTTAAGGGTCAAAGGATCTTAGACTTGGCCGTCAAGGCATGGAGGTGCCTCTCTGAGAGTGAGAAACAGCCATATATTGAGGCAAGTAAGAGGGTTAATGAAAAAAGAGCGGCTCAAGAAATGATGGCTGATGAGGAAAACCATGGCATGCAAAATGCAGAAAGAGGGAAGAAGCCTTCACAATCACACAACGGGAACTGCCCCGCGGCATTGCAATCTGATACGGAAAAATCCTGTGTTCAAAATCAAGCAGTGAATTTTCAAGAAAGTCTCCTAGAAGGGATCTACTGGAGTTCAACATCTTCAGAACAGAAACAATTCCCCCAGCTAAGTTCAAAGAGAAAAGAGATGAAAAAGCGTTGTGGCGCTCCTCGAGGGGCACAAAGTGGTTATCATATCTACCTCAGGAAAGAATGCGAAAGGCTGAAAAGTACTGATGCAGGGAAACTGAAGGGTCAAAATTTCCGAGCCATGGCAGACAATGCATGGAGATCCTTCTCTGAGACTGAGAAACTGCCATACATTGAGGCAAGTAAGAAGGTTAATGGAAAACGACTGGCTCAACAAGTGACTGCTGATGAGGAAAACCTGCGCATGCAAAATCTAGAGTTTCCGTTGGGGGTTGCCCTTGAAGTCGAAGAGGCTTTATATTCCGGTGAAAGGCGGATGCTATACAAAACGACAGGACATTATCTGCAGCCTGACTTTACATTCTGCAATCCGCACCTATTAGCCAAAATACTGGATAGCACAGCCTATGAGAGCAAAAAGATGAGCACATCCACCACCACAAATCTTCCTCACAACATCAAACCAAATGAATCCTCAGAAGATATCAAATCTCCCACCCCCAAGCTCTCCCAGAAAACAATCTTACACATCGTAAACACGAAATGCGCAACTTCGCTGCAACATCTCAAGCAAGCTCATGGTGTTGCCCTGAGGTCTGGCCACTTCCAAGACCATTACGTGGCCGGGGCTATCGTGAAATGCTACGCAAGCAACAACTTCGGGCTTGCGTTGAATGTCTTCGATTGCGTGTGGAGACCGAATGTGTTTGTGTGGAATATTGTGATCAAAGGGTGCCTAGAGCACAATGAGGCATTCAGTTGTGTGTCATATTATTGTAAGATGGTGGATATGAATGCCAGGCCTAATAAGTTCACGTATTCCATGTTGTTCAAAGCCTGTTCGTTGGCTCAAGCGGTGGAGGAAGCCCTGCAAATCCACGCGCATGTTGTTAAAAATCAGTTTGGTGAAGATGGGCACATAAGGAGCGCTGGGATTCAAATGTACGCGTCTTTTGGGCTCGTGGAGGAGGCAAGGATAATGTTCGATGAGGGTGGGGAAGCGAACGATGTTGTTTGTTGGAACGCGATGATTGATGGTTACATGAAATGTGGAGATGTAGAGGTAGCTAAAGCATTGTTTTATAAGCATATGGCGAGTAAAAATATCGGTTCTTGGAATGCAATGGTGAGCGGTCTTGCTAGGTGTGGCAGGATTAAAGAGGCGAGGGAGTTGTTTGACGGCATGAGCGAAAGGGACGAGATATCTTGGAGTGCTATGATTGATGGCTATATTCAAGGAGGGTTTCACAAGGAGGCCTTGGAAATTTTCAATGAGATGCAAAAGGAGAATCTAATTAGTCCGAAAAAATTCGTGTTGTCAAGTGTGCTAGCTGCTTGTGCTAATGTGGGAGCGCTTGATCAAGGAAAATGGATTCATGCTTACATCAAGAAGAACAATATTCAGTTGGATGCGGTTCTTGGGACTGCTTTGCTTGACATGTACGCAAAATGCGGGCGGATTGACATGGCGTGGGAGGTGTTTGAAAATGTCAAACAGAAAGAGATTTCGACATGGAATGCCATGATTGGAGCGCTTGCTATGCACGGCCGAGCAGAGGATGCAGTTGAACTTTTTGCCAAGATGCAGATGCGTAAGTTGGAACCAAATGGAATTACATTTTTGAATGCCTTAACTGCTTGTGCTCATTCAGGTTTTGTCGAGAAAGGTCTTGAAATTTTCAGTTCTATGAAGAGATTTTATGGCATTCAGCCTGAGGTGGAGCACTACGGGTGTGTGGTTGATATGTTTGGAAGGGCAGGGAAATTGGAAGAGGCAGAGCAGCTCATAAACTTGATGCCAATCGAACCCAATGCGGCAGTTTGGGGGGCGTTATTAGGCGCTTGCAGGATACATGGAAATGTCGAAATGGGAGAGAGAGTCGGAAAGATTTTGCTCGAATTGGAGCCTCAAAACAGCGGTCGCTACACATTGTTATCAAACATTTACGCGAAGGCAGGCAGGTTTGATGATGCTGCAAAGGTAAGAACTTTGATGAAGGAAAGAGGGGTGAAGACAAGTCCAGGGATTAGCATGGTCGACATTGGTGGCATTGTTCATGAATTCAAAGTGGGAGACGGATCACACCCGAATACTAAGGAGGTCTATTCGATGCTGGAGAGGATCATAGAGAAGCTGCAGACGGAGGGCTATTCGCTGAATAGCTCACAAGTCTTGTTTGACATTGCAGAGGAGGAGAAGGAAAGTGCATTGCAGTACCACAGTGAAAAGCTTGCCATTGCTTTCGGAGTCCTCAACACAAAACCCGGAACAGCCATTCGCGTCACGAAGAACTTGAGGATCTGTGAGGATTGCCATTCTGCAGTAAAGATCTTCTCAAAAGTATACGAAAGGGATGTGATCGTTAGGGACCGGATGCGGTATCATCATTTTAGAAATGGAAGGTGTTCATGTAAGGATTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

1002

Amino Acids

112.89

Weight (kDa)

8.62

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMG_box_2 PF09011 51 - 118 2.9e-06 HMG-box domain
PPR_2 PF13041 426 - 473 8.7e-10 PPR repeat family
PPR PF01535 564 - 591 1.9e-06 PPR repeat
PPR PF01535 594 - 623 7.2e-08 PPR repeat
PPR_2 PF13041 695 - 741 2.9e-09 PPR repeat family
PPR PF01535 696 - 724 1.5e-06 PPR repeat
E_motif PF20431 811 - 872 3e-20 E motif
DYW_deaminase PF14432 909 - 1001 1.3e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1662
AasI GACNNNNNNGTC 1 cut(s) 257
AatII GACGTC 1 cut(s) 142
AbsI CCTCGAGG 1 cut(s) 600
Acc16I TGCGCA 2 cut(s) 821, 1126
Acc36I ACCTGC 2 cut(s) 825, 2529
AccB1I GGYRCC 2 cut(s) 273, 1311
AccB7I CCANNNNNTGG 1 cut(s) 1198
AccBSI CCGCTC 3 cut(s) 202, 338, 1710
AccI GTMKAC 2 cut(s) 2616, 2922
AccII CGCG 6 cut(s) 434, 1460, 1526, 1604, 2528, 2859
AclWI GGATC 6 cut(s) 251, 518, 714, 2665, 2708, 2885
AcoI YGGCCR 4 cut(s) 255, 1177, 1200, 2125
AcsI RAATTY 8 cut(s) 10, 211, 484, 688, 1842, 1886, 2253, 2639
AcuI CTGAAG 3 cut(s) 204, 516, 698
AcyI GRCGYC 1 cut(s) 139
AdeI CACNNNGTG 2 cut(s) 167, 614
AfaI GTAC 4 cut(s) 661, 1523, 2021, 2795
AfeI AGCGCT 3 cut(s) 1507, 1932, 2112
AfiI CCNNNNNNNGG 4 cut(s) 700, 888, 1198, 2134
AflII CTTAAG 1 cut(s) 230
AflIII ACRYGT 2 cut(s) 1524, 2016
AjnI CCWGG 2 cut(s) 1376, 2599
Alw21I GWGCWC 4 cut(s) 1007, 1323, 2224, 2304
Alw26I GTCTC 4 cut(s) 503, 725, 1267, 2646
AlwI GGATC 6 cut(s) 251, 518, 714, 2665, 2708, 2885
Ama87I CYCGRG 1 cut(s) 600
Aor51HI AGCGCT 3 cut(s) 1507, 1932, 2112
AoxI GGCC 6 cut(s) 255, 1177, 1200, 1379, 1833, 2125
ApeKI GCWGC 7 cut(s) 109, 925, 1135, 1910, 2359, 2552, 2713
ApoI RAATTY 8 cut(s) 10, 211, 484, 688, 1842, 1886, 2253, 2639
ArsI GACNNNNNNTTYG 2 cut(s) 2482, 2514
Asp700I GAANNNNTTC 5 cut(s) 98, 545, 1256, 1886, 2983
AspLEI GCGC 8 cut(s) 596, 822, 1127, 1462, 1508, 1933, 2113, 2420
AspS9I GGNCC 1 cut(s) 2947
AsuC2I CCSGG 2 cut(s) 1204, 2844
AsuHPI GGTGA 4 cut(s) 896, 1496, 1717, 2599
AsuNHI GCTAGC 1 cut(s) 1906
AvaI CYCGRG 1 cut(s) 600
AvaII GGWCC 1 cut(s) 2947
AxyI CCTNAGG 4 cut(s) 185, 632, 1169, 2292
BaeGI GKGCMC 2 cut(s) 610, 1497
BalI TGGCCA 1 cut(s) 1179
BanI GGYRCC 2 cut(s) 273, 1311
BanII GRGCYC 1 cut(s) 1539
BauI CACGAG 2 cut(s) 153, 1538
BbsI GAAGAC 2 cut(s) 1249, 2597
Bbv12I GWGCWC 4 cut(s) 1007, 1323, 2224, 2304
BbvI GCAGC 7 cut(s) 96, 937, 1122, 1897, 2371, 2539, 2700
BccI CCATC 5 cut(s) 346, 1354, 1484, 1607, 1796
BceAI ACGGC 3 cut(s) 242, 1774, 2140
BcgI CGANNNNNNTGC 4 cut(s) 1219, 1253, 2372, 2406
BciT130I CCWGG 2 cut(s) 1378, 2601
BciVI GTATCC 1 cut(s) 2421
BclI TGATCA 2 cut(s) 1302, 1936
BcnI CCSGG 2 cut(s) 1204, 2844
BcoDI GTCTC 4 cut(s) 503, 725, 1267, 2646
BfaI CTAG 5 cut(s) 503, 833, 1316, 1719, 1907
BfmI CTRYAG 4 cut(s) 84, 923, 2714, 2898
BfoI RGCGCY 5 cut(s) 597, 1509, 1934, 2114, 2421
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 2 cut(s) 825, 2529
BfuI GTATCC 1 cut(s) 2421
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 1 cut(s) 2907
BmcAI AGTACT 1 cut(s) 661
Bme1390I CCNGG 4 cut(s) 1204, 1378, 2601, 2844
Bme18I GGWCC 1 cut(s) 2947
BmeT110I CYCGRG 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 2947
BmiI GGNNCC 6 cut(s) 101, 275, 1313, 2181, 2484, 2948
BmrFI CCNGG 4 cut(s) 1204, 1378, 2601, 2844
BmtI GCTAGC 1 cut(s) 1910
BpiI GAAGAC 2 cut(s) 1249, 2597
BpmI CTGGAG 2 cut(s) 538, 2714
BpuEI CTTGAG 4 cut(s) 327, 1130, 1413, 2893
BpuMI CCSGG 2 cut(s) 1204, 2844
BsaAI YACGTR 2 cut(s) 1198, 1395
BsaBI GATNNNNATC 1 cut(s) 2378
BsaHI GRCGYC 1 cut(s) 139
BsaI GGTCTC 1 cut(s) 1267
BsaJI CCNNGG 6 cut(s) 370, 432, 699, 1203, 1836, 2600
BsaWI WCCGGW 2 cut(s) 881, 2949
BsaXI ACNNNNNCTCC 2 cut(s) 2442, 2472
Bsc4I CCNNNNNNNGG 4 cut(s) 700, 888, 1198, 2134
Bse1I ACTGG 3 cut(s) 521, 789, 975
Bse21I CCTNAGG 4 cut(s) 185, 632, 1169, 2292
Bse3DI GCAATG 5 cut(s) 437, 1707, 2760, 2786, 2814
Bse8I GATNNNNATC 1 cut(s) 2378
BseBI CCWGG 2 cut(s) 1378, 2601
BseDI CCNNGG 6 cut(s) 370, 432, 699, 1203, 1836, 2600
BseGI GGATG 6 cut(s) 900, 1007, 1993, 2143, 2938, 2958
BseJI GATNNNNATC 1 cut(s) 2378
BseLI CCNNNNNNNGG 4 cut(s) 700, 888, 1198, 2134
BseMI GCAATG 5 cut(s) 437, 1707, 2760, 2786, 2814
BseMII CTCAG 8 cut(s) 199, 273, 646, 720, 726, 1068, 1160, 2283
BseNI ACTGG 3 cut(s) 521, 789, 975
BseRI GAGGAG 4 cut(s) 588, 1559, 2783, 2786
BseSI GKGCMC 2 cut(s) 610, 1497
BseX3I CGGCCG 1 cut(s) 2125
BseXI GCAGC 7 cut(s) 96, 937, 1122, 1897, 2371, 2539, 2700
BseYI CCCAGC 3 cut(s) 105, 552, 1508
BsgI GTGCAG 1 cut(s) 95
Bsh1236I CGCG 6 cut(s) 434, 1460, 1526, 1604, 2528, 2859
Bsh1285I CGRYCG 2 cut(s) 2128, 2500
BshFI GGCC 6 cut(s) 257, 1179, 1202, 1381, 1835, 2127
BshNI GGYRCC 2 cut(s) 273, 1311
BsiEI CGRYCG 2 cut(s) 2128, 2500
BsiHKAI GWGCWC 4 cut(s) 1007, 1323, 2224, 2304
BsiHKCI CYCGRG 1 cut(s) 600
BsiSI CCGG 4 cut(s) 882, 1203, 2844, 2950
BslFI GGGAC 4 cut(s) 172, 1787, 2014, 2960
BslI CCNNNNNNNGG 4 cut(s) 700, 888, 1198, 2134
BsmAI GTCTC 4 cut(s) 503, 725, 1267, 2646
BsmBI CGTCTC 1 cut(s) 2646
BsmFI GGGAC 4 cut(s) 172, 1787, 2014, 2960
BsmI GAATGC 7 cut(s) 647, 1331, 1378, 1702, 2101, 2209, 2283
BsnI GGCC 6 cut(s) 257, 1179, 1202, 1381, 1835, 2127
Bso31I GGTCTC 1 cut(s) 1267
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 7 cut(s) 610, 1007, 1323, 1497, 1539, 2224, 2304
Bsp19I CCATGG 2 cut(s) 370, 699
BspANI GGCC 6 cut(s) 257, 1179, 1202, 1381, 1835, 2127
BspCNI CTCAG 8 cut(s) 198, 274, 645, 721, 727, 1067, 1161, 2284
BspFNI CGCG 6 cut(s) 434, 1460, 1526, 1604, 2528, 2859
BspHI TCATGA 1 cut(s) 2635
BspLI GGNNCC 6 cut(s) 101, 275, 1313, 2181, 2484, 2948
BspMAI CTGCAG 4 cut(s) 88, 927, 2718, 2902
BspMI ACCTGC 2 cut(s) 825, 2529
BspOI GCTAGC 1 cut(s) 1910
BspPI GGATC 6 cut(s) 251, 518, 714, 2665, 2708, 2885
BspT107I GGYRCC 2 cut(s) 273, 1311
BspTI CTTAAG 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 1267
BsrBI CCGCTC 3 cut(s) 202, 338, 1710
BsrDI GCAATG 5 cut(s) 437, 1707, 2760, 2786, 2814
BsrI ACTGG 3 cut(s) 521, 789, 975
BssECI CCNNGG 6 cut(s) 370, 432, 699, 1203, 1836, 2600
BssNAI GTATAC 1 cut(s) 2923
BssNI GRCGYC 1 cut(s) 139
BssSI CACGAG 2 cut(s) 153, 1538
BssT1I CCWWGG 3 cut(s) 370, 699, 1836
Bst1107I GTATAC 1 cut(s) 2923
Bst2BI CACGAG 2 cut(s) 153, 1538
Bst2UI CCWGG 2 cut(s) 1378, 2601
Bst4CI ACNGT 1 cut(s) 2801
Bst6I CTCTTC 3 cut(s) 861, 2264, 2343
BstACI GRCGYC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 230
BstAPI GCANNNNNTGC 2 cut(s) 710, 2138
BstBAI YACGTR 2 cut(s) 1198, 1395
BstDSI CCRYGG 3 cut(s) 370, 432, 699
BstF5I GGATG 6 cut(s) 900, 1007, 1993, 2143, 2938, 2958
BstFNI CGCG 6 cut(s) 434, 1460, 1526, 1604, 2528, 2859
BstH2I RGCGCY 5 cut(s) 597, 1509, 1934, 2114, 2421
BstHHI GCGC 8 cut(s) 596, 822, 1127, 1462, 1508, 1933, 2113, 2420
BstMAI GTCTC 4 cut(s) 503, 725, 1267, 2646
BstMCI CGRYCG 2 cut(s) 2128, 2500
BstMWI GCNNNNNNNGC 7 cut(s) 263, 710, 1227, 1916, 2119, 2138, 2213
BstNI CCWGG 2 cut(s) 1378, 2601
BstNSI RCATGY 4 cut(s) 379, 826, 1466, 2020
BstSCI CCNGG 4 cut(s) 1202, 1376, 2599, 2842
BstSFI CTRYAG 4 cut(s) 84, 923, 2714, 2898
BstSLI GKGCMC 2 cut(s) 610, 1497
BstUI CGCG 6 cut(s) 434, 1460, 1526, 1604, 2528, 2859
BstV1I GCAGC 7 cut(s) 96, 937, 1122, 1897, 2371, 2539, 2700
BstV2I GAAGAC 2 cut(s) 1249, 2597
BstX2I RGATCY 5 cut(s) 243, 510, 719, 2877, 2907
BstXI CCANNNNNNTGG 1 cut(s) 970
BstYI RGATCY 5 cut(s) 243, 510, 719, 2877, 2907
BstZ17I GTATAC 1 cut(s) 2923
BstZI CGGCCG 1 cut(s) 2125
Bsu36I CCTNAGG 4 cut(s) 185, 632, 1169, 2292
BsuI GTATCC 1 cut(s) 2421
BsuRI GGCC 6 cut(s) 257, 1179, 1202, 1381, 1835, 2127
BtgI CCRYGG 3 cut(s) 370, 432, 699
BtgZI GCGATG 1 cut(s) 1619
BtsCI GGATG 6 cut(s) 900, 1007, 1993, 2143, 2938, 2958
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 486, 2806
BveI ACCTGC 2 cut(s) 825, 2529
CciI TCATGA 1 cut(s) 2635
CfoI GCGC 8 cut(s) 596, 822, 1127, 1462, 1508, 1933, 2113, 2420
Cfr13I GGNCC 1 cut(s) 2947
Cfr42I CCGCGG 1 cut(s) 435
CseI GACGC 2 cut(s) 1515, 2848
Csp6I GTAC 4 cut(s) 660, 1522, 2020, 2794
CviQI GTAC 4 cut(s) 660, 1522, 2020, 2794
DraIII CACNNNGTG 2 cut(s) 167, 614
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 4 cut(s) 255, 1177, 1200, 2125
EagI CGGCCG 1 cut(s) 2125
Eam1104I CTCTTC 3 cut(s) 861, 2264, 2343
EarI CTCTTC 3 cut(s) 861, 2264, 2343
EciI GGCGGA 2 cut(s) 907, 2050
EclXI CGGCCG 1 cut(s) 2125
Eco130I CCWWGG 3 cut(s) 370, 699, 1836
Eco147I AGGCCT 2 cut(s) 1381, 1835
Eco24I GRGCYC 1 cut(s) 1539
Eco31I GGTCTC 1 cut(s) 1267
Eco32I GATATC 2 cut(s) 1063, 1782
Eco47I GGWCC 1 cut(s) 2947
Eco47III AGCGCT 3 cut(s) 1507, 1932, 2112
Eco52I CGGCCG 1 cut(s) 2125
Eco57I CTGAAG 3 cut(s) 204, 516, 698
Eco81I CCTNAGG 4 cut(s) 185, 632, 1169, 2292
Eco88I CYCGRG 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 2639
EcoRII CCWGG 2 cut(s) 1376, 2599
EcoRV GATATC 2 cut(s) 1063, 1782
EcoT14I CCWWGG 3 cut(s) 370, 699, 1836
EcoT22I ATGCAT 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 1539
ErhI CCWWGG 3 cut(s) 370, 699, 1836
Esp3I CGTCTC 1 cut(s) 2646
FalI AAGNNNNNCTT 2 cut(s) 1820, 1852
FaqI GGGAC 4 cut(s) 172, 1787, 2014, 2960
FauI CCCGC 2 cut(s) 439, 2024
FauNDI CATATG 1 cut(s) 1668
FbaI TGATCA 2 cut(s) 1302, 1936
FblI GTMKAC 2 cut(s) 2616, 2922
FokI GGATG 6 cut(s) 907, 994, 2000, 2150, 2945, 2965
FriOI GRGCYC 1 cut(s) 1539
FspBI CTAG 5 cut(s) 503, 833, 1316, 1719, 1907
FspI TGCGCA 2 cut(s) 821, 1126
GlaI GCGC 8 cut(s) 595, 821, 1126, 1461, 1507, 1932, 2112, 2419
GsaI CCCAGC 3 cut(s) 109, 556, 1512
GsuI CTGGAG 2 cut(s) 538, 2714
HaeII RGCGCY 5 cut(s) 597, 1509, 1934, 2114, 2421
HaeIII GGCC 6 cut(s) 257, 1179, 1202, 1381, 1835, 2127
HapII CCGG 4 cut(s) 882, 1203, 2844, 2950
HgaI GACGC 2 cut(s) 1515, 2848
HhaI GCGC 8 cut(s) 596, 822, 1127, 1462, 1508, 1933, 2113, 2420
Hin1I GRCGYC 1 cut(s) 139
Hin6I GCGC 8 cut(s) 594, 820, 1125, 1460, 1506, 1931, 2111, 2418
HinP1I GCGC 8 cut(s) 594, 820, 1125, 1460, 1506, 1931, 2111, 2418
HincII GTYRAC 1 cut(s) 2617
HindII GTYRAC 1 cut(s) 2617
HindIII AAGCTT 1 cut(s) 2807
HinfI GANTC 6 cut(s) 1049, 1513, 1867, 1951, 2457, 2826
HpaII CCGG 4 cut(s) 882, 1203, 2844, 2950
HphI GGTGA 4 cut(s) 896, 1496, 1717, 2599
Hpy166II GTNNAC 4 cut(s) 1114, 1392, 2617, 2923
Hpy8I GTNNAC 4 cut(s) 1114, 1392, 2617, 2923
Hpy99I CGWCG 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 2801
HpyCH4IV ACGT 3 cut(s) 139, 1197, 1394
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 710, 1227, 1916, 2119, 2138, 2213
HpySE526I ACGT 3 cut(s) 139, 1197, 1394
Hsp92I GRCGYC 1 cut(s) 139
HspAI GCGC 8 cut(s) 594, 820, 1125, 1460, 1506, 1931, 2111, 2418
Ksp22I TGATCA 2 cut(s) 1302, 1936
KspI CCGCGG 1 cut(s) 435
LmnI GCTCC 6 cut(s) 601, 1503, 1928, 2108, 2299, 2482
Lsp1109I GCAGC 7 cut(s) 96, 937, 1122, 1897, 2371, 2539, 2700
MaeI CTAG 5 cut(s) 503, 833, 1316, 1719, 1907
MaeII ACGT 3 cut(s) 139, 1197, 1394
MaeIII GTNAC 4 cut(s) 3, 796, 1616, 2860
MbiI CCGCTC 3 cut(s) 202, 338, 1710
MflI RGATCY 5 cut(s) 243, 510, 719, 2877, 2907
MhlI GDGCHC 7 cut(s) 610, 1007, 1323, 1497, 1539, 2224, 2304
MlsI TGGCCA 1 cut(s) 1179
MluI ACGCGT 1 cut(s) 1524
MluNI TGGCCA 1 cut(s) 1179
MlyI GAGTC 2 cut(s) 2466, 2835
MmeI TCCRAC 4 cut(s) 1577, 1965, 2157, 2440
Mox20I TGGCCA 1 cut(s) 1179
Mph1103I ATGCAT 1 cut(s) 715
MroXI GAANNNNTTC 5 cut(s) 98, 545, 1256, 1886, 2983
MscI TGGCCA 1 cut(s) 1179
MseI TTAA 6 cut(s) 231, 324, 771, 1470, 1734, 2210
MslI CAYNNNNRTG 3 cut(s) 271, 1461, 2436
Msp20I TGGCCA 1 cut(s) 1179
MspA1I CMGCKG 3 cut(s) 109, 434, 2496
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 4 cut(s) 882, 1203, 2844, 2950
MspR9I CCNGG 4 cut(s) 1204, 1378, 2601, 2844
Mva1269I GAATGC 7 cut(s) 647, 1331, 1378, 1702, 2101, 2209, 2283
MvaI CCWGG 2 cut(s) 1378, 2601
MvnI CGCG 6 cut(s) 434, 1460, 1526, 1604, 2528, 2859
MwoI GCNNNNNNNGC 7 cut(s) 263, 710, 1227, 1916, 2119, 2138, 2213
NciI CCSGG 2 cut(s) 1204, 2844
NcoI CCATGG 2 cut(s) 370, 699
NdeI CATATG 1 cut(s) 1668
NheI GCTAGC 1 cut(s) 1906
NlaIV GGNNCC 6 cut(s) 101, 275, 1313, 2181, 2484, 2948
NmeAIII GCCGAG 1 cut(s) 2153
NmuCI GTSAC 2 cut(s) 796, 2860
NsbI TGCGCA 2 cut(s) 821, 1126
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 4 cut(s) 379, 826, 1466, 2020
PaeI GCATGC 2 cut(s) 379, 826
PaeR7I CTCGAG 1 cut(s) 600
PagI TCATGA 1 cut(s) 2635
PceI AGGCCT 2 cut(s) 1381, 1835
PciI ACATGT 1 cut(s) 2016
PcsI WCGNNNNNNNCGW 1 cut(s) 1764
PctI GAATGC 7 cut(s) 647, 1331, 1378, 1702, 2101, 2209, 2283
PdmI GAANNNNTTC 5 cut(s) 98, 545, 1256, 1886, 2983
PfeI GAWTC 4 cut(s) 1049, 1513, 1867, 1951
PflMI CCANNNNNTGG 1 cut(s) 1198
PleI GAGTC 2 cut(s) 2465, 2834
PpsI GAGTC 2 cut(s) 2465, 2834
Ppu21I YACGTR 2 cut(s) 1198, 1395
PscI ACATGT 1 cut(s) 2016
PsiI TTATAA 1 cut(s) 1662
Psp6I CCWGG 2 cut(s) 1376, 2599
PspFI CCCAGC 3 cut(s) 105, 552, 1508
PspGI CCWGG 2 cut(s) 1376, 2599
PspN4I GGNNCC 6 cut(s) 101, 275, 1313, 2181, 2484, 2948
PspPI GGNCC 1 cut(s) 2947
PspXI VCTCGAGB 1 cut(s) 600
PstI CTGCAG 4 cut(s) 88, 927, 2718, 2902
PsuI RGATCY 5 cut(s) 243, 510, 719, 2877, 2907
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 4 cut(s) 661, 1523, 2021, 2795
RsaNI GTAC 4 cut(s) 660, 1522, 2020, 2794
RseI CAYNNNNRTG 3 cut(s) 271, 1461, 2436
SacII CCGCGG 1 cut(s) 435
SalI GTCGAC 1 cut(s) 2615
SaqAI TTAA 6 cut(s) 231, 324, 771, 1470, 1734, 2210
Sau96I GGNCC 1 cut(s) 2947
ScaI AGTACT 1 cut(s) 661
SchI GAGTC 2 cut(s) 2466, 2835
ScrFI CCNGG 4 cut(s) 1204, 1378, 2601, 2844
SduI GDGCHC 7 cut(s) 610, 1007, 1323, 1497, 1539, 2224, 2304
SfcI CTRYAG 4 cut(s) 84, 923, 2714, 2898
Sfr274I CTCGAG 1 cut(s) 600
Sfr303I CCGCGG 1 cut(s) 435
SgrBI CCGCGG 1 cut(s) 435
SinI GGWCC 1 cut(s) 2947
SlaI CTCGAG 1 cut(s) 600
SmiMI CAYNNNNRTG 3 cut(s) 271, 1461, 2436
SmlI CTYRAG 6 cut(s) 230, 342, 600, 1145, 1428, 2872
SmoI CTYRAG 6 cut(s) 230, 342, 600, 1145, 1428, 2872
SphI GCATGC 2 cut(s) 379, 826
SseBI AGGCCT 2 cut(s) 1381, 1835
SspI AATATT 2 cut(s) 1297, 1978
SspMI CTAG 5 cut(s) 503, 833, 1316, 1719, 1907
StuI AGGCCT 2 cut(s) 1381, 1835
StyD4I CCNGG 4 cut(s) 1202, 1376, 2599, 2842
StyI CCWWGG 3 cut(s) 370, 699, 1836
TaaI ACNGT 1 cut(s) 2801
TaiI ACGT 3 cut(s) 142, 1200, 1397
TaqII GACCGA 1 cut(s) 1291
TatI WGTACW 1 cut(s) 659
TauI GCSGC 3 cut(s) 341, 437, 2396
TfiI GAWTC 4 cut(s) 1049, 1513, 1867, 1951
Tru1I TTAA 6 cut(s) 231, 324, 771, 1470, 1734, 2210
Tru9I TTAA 6 cut(s) 231, 324, 771, 1470, 1734, 2210
TscAI CASTG 2 cut(s) 486, 2806
TseFI GTSAC 2 cut(s) 796, 2860
TseI GCWGC 7 cut(s) 109, 925, 1135, 1910, 2359, 2552, 2713
Tsp45I GTSAC 2 cut(s) 796, 2860
TspGWI ACGGA 4 cut(s) 467, 831, 2670, 2735
TspRI CASTG 2 cut(s) 486, 2806
Van91I CCANNNNNTGG 1 cut(s) 1198
Vha464I CTTAAG 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 2947
XapI RAATTY 8 cut(s) 10, 211, 484, 688, 1842, 1886, 2253, 2639
XbaI TCTAGA 1 cut(s) 832
XceI RCATGY 4 cut(s) 379, 826, 1466, 2020
XhoI CTCGAG 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 2616, 2922
XmnI GAANNNNTTC 5 cut(s) 98, 545, 1256, 1886, 2983
XspI CTAG 5 cut(s) 503, 833, 1316, 1719, 1907
ZraI GACGTC 1 cut(s) 140
ZrmI AGTACT 1 cut(s) 661
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.