pycom12g22400

Phosphoribosylaminoimidazole carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008753
Physical Location & Seq
Forward (+)
23295087 .. 23296293
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g22400.1

Sequence Viewer

Length: 627 bp
ATGCTTCAGCAGAGCTCTAGCTGCTGTGACTCCCTCTTCGCGTCGCCGGATTTCGAGTTTAGGCCGTGTTTGACCTCGACGAAAACCAAAAACGCATTCTCCTGCTCCATGGAAAAGCACAAGCTGCTTTTCACTTCTGCTTCTTCTCTCTCGCTCAAGCAGCGCTTCCAAACGAAGCGCAATCCGGTGCTCGCCTGCCGAGCATCACGTGACCCTCGAGTGACTTCTGGGAAAGATGATGCTGCGTTCCATGGAGTTGCGGATTTGATTGTTGGTGTTCTTGGAGGAGGCCAACTGGGTCGGATGCTCTGCCAAGCAGCTTCGCAAATGGCGATTAAAGTGATGGTATTGGACCCACAAGAGAACTGCCCGGCTAGTGAGATTTCCCATCATCATATGGTTGGAAGCTTCGATGATAGTGCAACGGTCCAGGAATTCGCAAAGAGGTGTGGAGTGCTGACTGTGGAAATTGAGCATGTGGATGTGGAGACTTTGGAGAAGCTTGAGCAGCAAGGAGTGGATTGCCAACCCAAAGCCTCTACAATCAGAATAATTCAGGATAAGTATCTCCAAAAGGTTCATTTTTCGAAGCATGATATTCCCCTTCCTGAATTCATGCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 ko01110 map00230 map01100 map01110
Pfam Domains
Protein Families
AIRC ATP-grasp

Protein Analysis

209

Amino Acids

23.06

Weight (kDa)

7.06

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS_preATP-grasp-like PF22660 91 - 186 3.3e-33 Ribonucleotide synthetase preATP-grasp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 41
AciI CCGC 1 cut(s) 260
AcsI RAATTY 2 cut(s) 434, 611
AcvI CACGTG 1 cut(s) 209
AfeI AGCGCT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 429
AjuI GAANNNNNNNTTGG 2 cut(s) 80, 112
AluBI AGCT 6 cut(s) 15, 21, 124, 320, 408, 502
AluI AGCT 6 cut(s) 15, 21, 124, 320, 408, 502
Alw21I GWGCWC 2 cut(s) 17, 192
Alw26I GTCTC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 216
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 2 cut(s) 62, 289
ApeKI GCWGC 6 cut(s) 21, 124, 160, 242, 317, 508
ApoI RAATTY 2 cut(s) 434, 611
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AspLEI GCGC 2 cut(s) 165, 180
AspS9I GGNCC 2 cut(s) 352, 427
AsuC2I CCSGG 1 cut(s) 371
AsuII TTCGAA 1 cut(s) 587
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 2 cut(s) 352, 427
BanII GRGCYC 1 cut(s) 17
BbrPI CACGTG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 17, 192
BbvI GCAGC 6 cut(s) 8, 111, 172, 229, 329, 520
BccI CCATC 2 cut(s) 337, 396
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 431
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 2 cut(s) 18, 375
BfoI RGCGCY 1 cut(s) 166
BisI GCNGC 6 cut(s) 22, 125, 161, 243, 318, 509
BlsI GCNGC 6 cut(s) 23, 126, 162, 244, 319, 510
Bme1390I CCNGG 2 cut(s) 371, 431
Bme18I GGWCC 2 cut(s) 352, 427
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 2 cut(s) 352, 427
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 2 cut(s) 371, 431
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 3 cut(s) 212, 229, 294
BmuI ACTGGG 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 2 cut(s) 140, 524
BpuMI CCSGG 1 cut(s) 371
BsaAI YACGTR 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 2 cut(s) 108, 250
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse1I ACTGG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 108, 250
BseGI GGATG 2 cut(s) 309, 487
BseJI GATNNNNATC 1 cut(s) 564
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 300
BseXI GCAGC 6 cut(s) 8, 111, 172, 229, 329, 520
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 2 cut(s) 64, 291
BsiHKAI GWGCWC 2 cut(s) 17, 192
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 3 cut(s) 47, 185, 371
BsmAI GTCTC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 2 cut(s) 64, 291
BsoBI CYCGRG 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 17, 192
Bsp19I CCATGG 2 cut(s) 108, 250
BspACI CCGC 1 cut(s) 260
BspANI GGCC 2 cut(s) 64, 291
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 1 cut(s) 354
BspT104I TTCGAA 1 cut(s) 587
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 2 cut(s) 108, 250
BssT1I CCWWGG 2 cut(s) 108, 250
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 2 cut(s) 427, 463
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 124
BstBAI YACGTR 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 192, 196
BstDSI CCRYGG 2 cut(s) 108, 250
BstF5I GGATG 2 cut(s) 309, 487
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 2 cut(s) 165, 180
BstMAI GTCTC 1 cut(s) 482
BstMWI GCNNNNNNNGC 5 cut(s) 21, 124, 160, 200, 508
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 369, 429
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 6 cut(s) 8, 111, 172, 229, 329, 520
BsuRI GGCC 2 cut(s) 64, 291
BtgI CCRYGG 2 cut(s) 108, 250
BtsCI GGATG 2 cut(s) 309, 487
Cac8I GCNNGC 2 cut(s) 192, 196
CfoI GCGC 2 cut(s) 165, 180
Cfr13I GGNCC 2 cut(s) 352, 427
CseI GACGC 1 cut(s) 30
CviAII CATG 5 cut(s) 109, 251, 476, 593, 616
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 2 cut(s) 108, 250
Eco24I GRGCYC 1 cut(s) 17
Eco47I GGWCC 2 cut(s) 352, 427
Eco47III AGCGCT 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 15
Eco72I CACGTG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 15
EcoRI GAATTC 2 cut(s) 434, 611
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 108, 250
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 2 cut(s) 108, 250
FaeI CATG 5 cut(s) 112, 254, 479, 596, 619
FaiI YATR 7 cut(s) 110, 252, 396, 398, 477, 594, 617
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FatI CATG 5 cut(s) 108, 250, 475, 592, 615
FauNDI CATATG 1 cut(s) 396
Fnu4HI GCNGC 6 cut(s) 22, 125, 161, 243, 318, 509
FokI GGATG 2 cut(s) 316, 494
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 6 cut(s) 22, 125, 161, 243, 318, 509
FspBI CTAG 2 cut(s) 18, 375
GlaI GCGC 2 cut(s) 164, 179
GluI GCNGC 6 cut(s) 22, 125, 161, 243, 318, 509
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 2 cut(s) 64, 291
HapII CCGG 3 cut(s) 47, 185, 371
HgaI GACGC 1 cut(s) 30
HhaI GCGC 2 cut(s) 165, 180
Hin1II CATG 5 cut(s) 112, 254, 479, 596, 619
Hin6I GCGC 2 cut(s) 163, 178
HinP1I GCGC 2 cut(s) 163, 178
HindIII AAGCTT 2 cut(s) 406, 500
HinfI GANTC 1 cut(s) 29
HpaII CCGG 3 cut(s) 47, 185, 371
Hpy188I TCNGA 2 cut(s) 303, 548
Hpy188III TCNNGA 2 cut(s) 557, 608
Hpy99I CGWCG 2 cut(s) 46, 82
HpyAV CCTTC 1 cut(s) 614
HpyCH4III ACNGT 2 cut(s) 427, 463
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 2 cut(s) 422, 619
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 124, 160, 200, 508
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 5 cut(s) 112, 254, 479, 596, 619
HspAI GCGC 2 cut(s) 163, 178
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 6 cut(s) 8, 111, 172, 229, 329, 520
LweI GCATC 3 cut(s) 212, 229, 294
MaeI CTAG 2 cut(s) 18, 375
MaeII ACGT 1 cut(s) 208
MaeIII GTNAC 3 cut(s) 26, 209, 220
MboII GAAGA 2 cut(s) 28, 135
MhlI GDGCHC 2 cut(s) 17, 192
MluCI AATT 4 cut(s) 434, 468, 552, 611
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 281, 382
MnlI CCTC 7 cut(s) 44, 85, 225, 278, 281, 438, 547
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 480
MspI CCGG 3 cut(s) 47, 185, 371
MspR9I CCNGG 2 cut(s) 371, 431
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 5 cut(s) 21, 124, 160, 200, 508
NciI CCSGG 1 cut(s) 371
NcoI CCATGG 2 cut(s) 108, 250
NdeI CATATG 1 cut(s) 396
NlaIII CATG 5 cut(s) 112, 254, 479, 596, 619
NlaIV GGNNCC 1 cut(s) 354
NmeAIII GCCGAG 1 cut(s) 224
NmuCI GTSAC 3 cut(s) 26, 209, 220
NspI RCATGY 1 cut(s) 479
NspV TTCGAA 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 214, 329
PctI GAATGC 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 6 cut(s) 23, 126, 162, 244, 319, 510
PleI GAGTC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
PpsI GAGTC 1 cut(s) 23
Ppu21I YACGTR 1 cut(s) 209
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 429
PspCI CACGTG 1 cut(s) 209
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 2 cut(s) 352, 427
PspXI VCTCGAGB 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 480
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 6 cut(s) 22, 125, 161, 243, 318, 509
Sau96I GGNCC 2 cut(s) 352, 427
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 371, 431
SduI GDGCHC 2 cut(s) 17, 192
SfaNI GCATC 3 cut(s) 212, 229, 294
Sfr274I CTCGAG 1 cut(s) 216
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 2 cut(s) 352, 427
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 3 cut(s) 155, 216, 503
SmoI CTYRAG 3 cut(s) 155, 216, 503
Sse9I AATT 4 cut(s) 434, 468, 552, 611
SsiI CCGC 1 cut(s) 260
SspMI CTAG 2 cut(s) 18, 375
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 369, 429
StyI CCWWGG 2 cut(s) 108, 250
TaaI ACNGT 2 cut(s) 427, 463
TaiI ACGT 1 cut(s) 211
TaqI TCGA 5 cut(s) 54, 77, 217, 411, 587
TasI AATT 4 cut(s) 434, 468, 552, 611
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseFI GTSAC 3 cut(s) 26, 209, 220
TseI GCWGC 6 cut(s) 21, 124, 160, 242, 317, 508
Tsp45I GTSAC 3 cut(s) 26, 209, 220
TspDTI ATGAA 2 cut(s) 569, 604
VpaK11BI GGWCC 2 cut(s) 352, 427
XapI RAATTY 2 cut(s) 434, 611
XceI RCATGY 1 cut(s) 479
XhoI CTCGAG 1 cut(s) 216
XspI CTAG 2 cut(s) 18, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.