Rroxscaffold_6G00428090

Phosphoribosylaminoimidazole carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008753
Physical Location & Seq
Reverse (-)
48582159 .. 48587645
5487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428090.1

Sequence Viewer

Length: 2025 bp
ATGCTTCAGCAGAGCTCCCTCTTCGCCACCGCCGATATCGGGTTCAGGCCGTGTTTGGTTCTCCCCAATAATACCAAAAACCCATTCTTCTCTTCCTCCATGGACCTCAAACCAACCCACGCCAACCTCCCAACTTTTGCTCCTGCTTCTTCTTCTTCTCGCTCACTAAACCAGGCCTTGGCTTGCCGGGCGTCACGTGACTCTCACATGACCCCTCCTCCCAGTGGAGATGGTTTAGCTGCTGTTGTTCATGGATTGTCTGAGACCATTGTGGGTGTTCTTGGAGGAGGCCAGCTGGGTCGGATGCTGTGCCAAGCAGCTTCACAAATGGCTATCAAGGTGATGGTATTGGACCCACAACAGAATTGCCCCGCGAGTAGACTCTCCTATCACCATATGGTTGGGAGCTTTGATGACAGTGCCACTGTCCAGGAATTCGCGAAAAGGTGTGGAGTGTTGACTGTGGAAATCGAACATGTGGATGTGGAGACATTGGAGAAGCTTGAGCAGCAAGGAGTAGACTGCCAGCCAAAAGCCTCTACAATCAGAATAATACAGGATAAATATCTTCAGAAGGTTCACTTTTCGCGGCATGCTATTCCACTTCCTGAATATATGCAGATAGATGATCTGGAAGGTGCCAAGAGGGCAGGCAACGTCTTTGACTATCCTTTAATGATAAAGAGTAAAAGATTAGCTTATGATGGGCGTGGAAATGCTGTTGCTAAGAGTGAGCATGATCTTTCTTCTGCTGTAACAGAAGTAGAACCTATTGCCCTTCTTTTACTCTCTAAGTTTGTTGCAGCTCTGGGAGGGTTTGATCGCGGTTTGTATGTTGAGAAATGGGCCCCTTTTGTAAAGCAGGAGCTGGCCGTCATTGTTGCTAGAGGAAGAGACAATTCTATCGTGTGCTATCCTGTTGTTGAAACTATACACAAGGAAAACATTTGTCACATTGTCAAAGCACCTGCTAACGTGCCATGGAAGATCCGAAAGTTGGCCACTGACATTGCATCTAGAGCTATTAGTTCATTAGAAGGCGCTGGTGTGTTTGCAGTTGAGTTGTTCTTGACAGAGGATGATCAGATATCTGCTTCATTCAACCAAGTTATACATGTATTCGCTTCTTTTCAGATTTTGCTAAATGAAGTAGCTCCCAGACCTCACAATAGCGGTCATCACACAATAGAGTCTTGCTACACCTCACAGTATGAACAGCATTTGCGGGCTGTTGTTGGTCTTCCACTTGGTGATCCATCAATGAAAACTCCTGCTGCAATTATGTACAATTTACTTGGTGAAGATGAGGGGGAAGCTGGTTTCCACCTAGCTCATCAAGTGATTGGAAGAGCCTTGAGTATTCCAGGGGCTACTGTTCATTGGTATGATAAGCCAGACATGCGGAAGCAACGGAAAATGGGTCACATTACCATTGTTGGACCTTCCATGGGCAATGTGGAAAAAGCTCTGGATTCAATGCTAAATGACAAAATATCTGATAGTCAGTCCGCTGCCACACCGCGTGTTGGGATCATAATGGGTTCTGATTCAGATCTTCCTGTTATGAAGGATGCTGCAAAAGTTTTGAATATGTTTGAAGTGCCTCACGAGGTTAGGATAGTTTCTGCGCATCGAACTCCCGATTGGATGCATTCTTATGCCTTGTCTGCCCAGGAAAGGGGAATTCAGGTCATCATTGCTGGTGCTGGTGGCGCAGCTCACTTGCCAGGAATGGTAGCTGCATCCACTCCATTACCCATTATTGGTGTCCCAGTGCGTGCCTCTACATTGGATGGAGTTGATTCGGTTCTATCTATATTGCAGATGCCAAGAGGGGTCCCGGTTGCAACAGTTGCTATAAACAATGCCACCAATGCTGGTTTGCTGGCAGTAAGGATGTTAGCTGTTTGTGATGCTGATCTACTTGCAAGAATCAGCCAGTATCAAGAAGACACAAAGAGCGAAGTCTTGGCAAAAGATGAGAAGCTACAGAAAGTTGGTTGGGAATCTTATTTGAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 ko01110 map00230 map01100 map01110
Pfam Domains
Protein Families
AIRC ATP-grasp

Protein Analysis

674

Amino Acids

72.98

Weight (kDa)

6.25

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS_preATP-grasp-like PF22660 91 - 186 1.4e-31 Ribonucleotide synthetase preATP-grasp domain
ATP-grasp PF02222 194 - 404 2.2e-51 ATP-grasp domain
PurK_C PF17769 426 - 488 5e-18 Phosphoribosylaminoimidazole carboxylase C-terminal domain
AIRC PF00731 508 - 653 2e-63 AIR carboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 976
Acc16I TGCGCA 1 cut(s) 1629
Acc36I ACCTGC 1 cut(s) 976
AccB1I GGYRCC 1 cut(s) 638
AccB7I CCANNNNNTGG 1 cut(s) 178
AccI GTMKAC 2 cut(s) 379, 519
AccII CGCG 5 cut(s) 374, 440, 589, 825, 1522
AciI CCGC 9 cut(s) 30, 372, 589, 825, 1173, 1225, 1402, 1509, 1520
AclWI GGATC 3 cut(s) 982, 1247, 1538
AcoI YGGCCR 2 cut(s) 870, 999
AcsI RAATTY 2 cut(s) 434, 1683
AcuI CTGAAG 1 cut(s) 554
AcvI CACGTG 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 191
AdeI CACNNNGTG 2 cut(s) 1250, 1523
AfaI GTAC 1 cut(s) 1286
AfiI CCNNNNNNNGG 9 cut(s) 39, 178, 224, 997, 1448, 1526, 1677, 1678, 1763
AflIII ACRYGT 2 cut(s) 475, 1114
AgsI TTSAA 6 cut(s) 926, 1102, 1476, 1588, 1598, 2017
AjnI CCWGG 5 cut(s) 171, 429, 1363, 1671, 1726
AjuI GAANNNNNNNTTGG 4 cut(s) 68, 100, 1627, 1659
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 3 cut(s) 257, 482, 888
AlwI GGATC 3 cut(s) 982, 1247, 1538
AlwNI CAGNNNCTG 1 cut(s) 868
AoxI GGCC 6 cut(s) 47, 174, 289, 846, 870, 999
ApaI GGGCCC 1 cut(s) 850
ApeKI GCWGC 9 cut(s) 239, 317, 508, 803, 1274, 1511, 1574, 1715, 1739
ApoI RAATTY 2 cut(s) 434, 1683
AspLEI GCGC 3 cut(s) 1043, 1630, 1715
AspS9I GGNCC 6 cut(s) 103, 352, 846, 847, 1439, 1837
AsuC2I CCSGG 2 cut(s) 188, 1841
AsuHPI GGTGA 4 cut(s) 352, 383, 1262, 1310
AvaII GGWCC 4 cut(s) 103, 352, 1439, 1837
BaeGI GKGCMC 1 cut(s) 850
BalI TGGCCA 1 cut(s) 1001
BanI GGYRCC 1 cut(s) 638
BanII GRGCYC 2 cut(s) 17, 850
BauI CACGAG 1 cut(s) 1607
BbrPI CACGTG 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 1232, 1956
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 9 cut(s) 226, 329, 520, 815, 1261, 1498, 1561, 1726, 1727
BccI CCATC 5 cut(s) 224, 337, 698, 1264, 1787
BceAI ACGGC 2 cut(s) 34, 857
BciT130I CCWGG 5 cut(s) 173, 431, 1365, 1673, 1728
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 2 cut(s) 188, 1841
BcoDI GTCTC 3 cut(s) 257, 482, 888
BfaI CTAG 3 cut(s) 885, 1017, 1328
BfmI CTRYAG 1 cut(s) 1988
BfoI RGCGCY 1 cut(s) 1044
BfuAI ACCTGC 1 cut(s) 976
BglI GCCNNNNNGGC 1 cut(s) 647
BglII AGATCT 1 cut(s) 1552
Bme1390I CCNGG 7 cut(s) 173, 188, 431, 1365, 1673, 1728, 1841
Bme18I GGWCC 4 cut(s) 103, 352, 1439, 1837
BmgT120I GGNCC 6 cut(s) 103, 352, 846, 847, 1439, 1837
BmiI GGNNCC 6 cut(s) 354, 640, 848, 849, 1838, 1839
BmrFI CCNGG 7 cut(s) 173, 188, 431, 1365, 1673, 1728, 1841
BmrI ACTGGG 2 cut(s) 216, 1766
BmsI GCATC 8 cut(s) 294, 1022, 1561, 1638, 1639, 1751, 1815, 1903
BmuI ACTGGG 2 cut(s) 216, 1766
BpiI GAAGAC 2 cut(s) 1232, 1956
BpuEI CTTGAG 2 cut(s) 524, 1375
BpuMI CCSGG 2 cut(s) 188, 1841
BsaAI YACGTR 1 cut(s) 197
BsaBI GATNNNNATC 3 cut(s) 564, 1551, 1917
BsaHI GRCGYC 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 6 cut(s) 99, 177, 980, 1364, 1446, 1671
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 202, 232
Bsc4I CCNNNNNNNGG 9 cut(s) 39, 178, 224, 997, 1448, 1526, 1677, 1678, 1763
Bse1I ACTGG 3 cut(s) 222, 1772, 1939
Bse3DI GCAATG 3 cut(s) 1008, 1459, 1695
Bse8I GATNNNNATC 3 cut(s) 564, 1551, 1917
BseBI CCWGG 5 cut(s) 173, 431, 1365, 1673, 1728
BseDI CCNNGG 6 cut(s) 99, 177, 980, 1364, 1446, 1671
BseGI GGATG 8 cut(s) 309, 487, 1084, 1576, 1653, 1742, 1798, 1902
BseJI GATNNNNATC 3 cut(s) 564, 1551, 1917
BseLI CCNNNNNNNGG 9 cut(s) 39, 178, 224, 997, 1448, 1526, 1677, 1678, 1763
BseMI GCAATG 3 cut(s) 1008, 1459, 1695
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 3 cut(s) 222, 1772, 1939
BseRI GAGGAG 2 cut(s) 207, 300
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 9 cut(s) 226, 329, 520, 815, 1261, 1498, 1561, 1726, 1727
BseYI CCCAGC 1 cut(s) 295
Bsh1236I CGCG 5 cut(s) 374, 440, 589, 825, 1522
BshFI GGCC 6 cut(s) 49, 176, 291, 848, 872, 1001
BshNI GGYRCC 1 cut(s) 638
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 2 cut(s) 187, 1841
BslFI GGGAC 2 cut(s) 1754, 1823
BslI CCNNNNNNNGG 9 cut(s) 39, 178, 224, 997, 1448, 1526, 1677, 1678, 1763
BsmAI GTCTC 3 cut(s) 257, 482, 888
BsmFI GGGAC 2 cut(s) 1754, 1823
BsmI GAATGC 1 cut(s) 1651
BsnI GGCC 6 cut(s) 49, 176, 291, 848, 872, 1001
Bso31I GGTCTC 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 846
Bsp1286I GDGCHC 2 cut(s) 17, 850
Bsp1407I TGTACA 1 cut(s) 1284
Bsp143I GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
Bsp19I CCATGG 3 cut(s) 99, 980, 1446
Bsp68I TCGCGA 1 cut(s) 440
BspACI CCGC 9 cut(s) 30, 372, 589, 825, 1173, 1225, 1402, 1509, 1520
BspANI GGCC 6 cut(s) 49, 176, 291, 848, 872, 1001
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 5 cut(s) 374, 440, 589, 825, 1522
BspLI GGNNCC 6 cut(s) 354, 640, 848, 849, 1838, 1839
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 3 cut(s) 982, 1247, 1538
BspQI GCTCTTC 1 cut(s) 1342
BspT107I GGYRCC 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 3 cut(s) 1008, 1459, 1695
BsrGI TGTACA 1 cut(s) 1284
BsrI ACTGG 3 cut(s) 222, 1772, 1939
BssECI CCNNGG 6 cut(s) 99, 177, 980, 1364, 1446, 1671
BssMI GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
BssNI GRCGYC 1 cut(s) 191
BssSI CACGAG 1 cut(s) 1607
BssT1I CCWWGG 4 cut(s) 99, 177, 980, 1446
Bst2BI CACGAG 1 cut(s) 1607
Bst2UI CCWGG 5 cut(s) 173, 431, 1365, 1673, 1728
Bst4CI ACNGT 6 cut(s) 419, 427, 463, 1209, 1375, 1852
Bst6I CTCTTC 4 cut(s) 26, 97, 886, 1342
BstACI GRCGYC 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 1853
BstAUI TGTACA 1 cut(s) 1284
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 9 cut(s) 184, 293, 527, 594, 652, 870, 1227, 1779, 1887
BstDEI CTNAG 3 cut(s) 261, 726, 792
BstDSI CCRYGG 3 cut(s) 99, 980, 1446
BstF5I GGATG 8 cut(s) 309, 487, 1084, 1576, 1653, 1742, 1798, 1902
BstFNI CGCG 5 cut(s) 374, 440, 589, 825, 1522
BstH2I RGCGCY 1 cut(s) 1044
BstHHI GCGC 3 cut(s) 1043, 1630, 1715
BstKTI GATC 9 cut(s) 631, 742, 823, 990, 1084, 1255, 1533, 1555, 1921
BstMAI GTCTC 3 cut(s) 257, 482, 888
BstMBI GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
BstMWI GCNNNNNNNGC 9 cut(s) 188, 508, 647, 1019, 1399, 1667, 1712, 1853, 1874
BstNI CCWGG 5 cut(s) 173, 431, 1365, 1673, 1728
BstNSI RCATGY 4 cut(s) 479, 596, 1118, 1402
BstSCI CCNGG 7 cut(s) 171, 186, 429, 1363, 1671, 1726, 1839
BstSFI CTRYAG 1 cut(s) 1988
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 5 cut(s) 374, 440, 589, 825, 1522
BstV1I GCAGC 9 cut(s) 226, 329, 520, 815, 1261, 1498, 1561, 1726, 1727
BstV2I GAAGAC 2 cut(s) 1232, 1956
BstX2I RGATCY 2 cut(s) 987, 1552
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 2 cut(s) 987, 1552
BsuRI GGCC 6 cut(s) 49, 176, 291, 848, 872, 1001
BtgI CCRYGG 3 cut(s) 99, 980, 1446
BtsCI GGATG 8 cut(s) 309, 487, 1084, 1576, 1653, 1742, 1798, 1902
BtsIMutI CAGTG 5 cut(s) 229, 423, 424, 1002, 1779
BtuMI TCGCGA 1 cut(s) 440
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 9 cut(s) 184, 293, 527, 594, 652, 870, 1227, 1779, 1887
CaiI CAGNNNCTG 1 cut(s) 868
CfoI GCGC 3 cut(s) 1043, 1630, 1715
Cfr13I GGNCC 6 cut(s) 103, 352, 846, 847, 1439, 1837
CseI GACGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 1285
CspCI CAANNNNNGTGG 2 cut(s) 991, 1026
CviQI GTAC 1 cut(s) 1285
DdeI CTNAG 3 cut(s) 261, 726, 792
DpnI GATC 9 cut(s) 630, 741, 822, 989, 1083, 1254, 1532, 1554, 1920
DpnII GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
DraIII CACNNNGTG 2 cut(s) 1250, 1523
EaeI YGGCCR 2 cut(s) 870, 999
Eam1104I CTCTTC 4 cut(s) 26, 97, 886, 1342
EarI CTCTTC 4 cut(s) 26, 97, 886, 1342
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 4 cut(s) 99, 177, 980, 1446
Eco147I AGGCCT 1 cut(s) 176
Eco24I GRGCYC 2 cut(s) 17, 850
Eco31I GGTCTC 1 cut(s) 257
Eco32I GATATC 2 cut(s) 37, 1089
Eco47I GGWCC 4 cut(s) 103, 352, 1439, 1837
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 1 cut(s) 554
Eco72I CACGTG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 15
EcoO109I RGGNCCY 2 cut(s) 847, 1837
EcoRI GAATTC 2 cut(s) 434, 1683
EcoRII CCWGG 5 cut(s) 171, 429, 1363, 1671, 1726
EcoRV GATATC 2 cut(s) 37, 1089
EcoT14I CCWWGG 4 cut(s) 99, 177, 980, 1446
EcoT22I ATGCAT 1 cut(s) 1653
EcoT38I GRGCYC 2 cut(s) 17, 850
ErhI CCWWGG 4 cut(s) 99, 177, 980, 1446
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 682, 714
FaqI GGGAC 2 cut(s) 1754, 1823
FauI CCCGC 2 cut(s) 379, 1218
FauNDI CATATG 1 cut(s) 396
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 2 cut(s) 379, 519
FokI GGATG 8 cut(s) 316, 494, 1091, 1583, 1660, 1729, 1805, 1909
FriOI GRGCYC 2 cut(s) 17, 850
FspBI CTAG 3 cut(s) 885, 1017, 1328
FspI TGCGCA 1 cut(s) 1629
GlaI GCGC 3 cut(s) 1042, 1629, 1714
GsaI CCCAGC 1 cut(s) 299
HaeII RGCGCY 1 cut(s) 1044
HaeIII GGCC 6 cut(s) 49, 176, 291, 848, 872, 1001
HapII CCGG 2 cut(s) 187, 1841
HgaI GACGC 1 cut(s) 180
HhaI GCGC 3 cut(s) 1043, 1630, 1715
Hin1I GRCGYC 1 cut(s) 191
Hin6I GCGC 3 cut(s) 1041, 1628, 1713
HinP1I GCGC 3 cut(s) 1041, 1628, 1713
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 9 cut(s) 200, 381, 1190, 1472, 1547, 1802, 1932, 2006, 2017
HpaII CCGG 2 cut(s) 187, 1841
HphI GGTGA 4 cut(s) 352, 383, 1262, 1310
Hpy166II GTNNAC 4 cut(s) 380, 459, 520, 580
Hpy188III TCNNGA 9 cut(s) 439, 608, 632, 1017, 1069, 1469, 1607, 1640, 1946
Hpy8I GTNNAC 4 cut(s) 380, 459, 520, 580
HpyAV CCTTC 6 cut(s) 568, 629, 788, 1031, 1452, 1561
HpyCH4III ACNGT 6 cut(s) 419, 427, 463, 1209, 1375, 1852
HpyCH4IV ACGT 3 cut(s) 196, 657, 975
HpyF10VI GCNNNNNNNGC 9 cut(s) 188, 508, 647, 1019, 1399, 1667, 1712, 1853, 1874
HpyF3I CTNAG 3 cut(s) 261, 726, 792
HpySE526I ACGT 3 cut(s) 196, 657, 975
Hsp92I GRCGYC 1 cut(s) 191
HspAI GCGC 3 cut(s) 1041, 1628, 1713
KflI GGGWCCC 1 cut(s) 1837
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
LguI GCTCTTC 1 cut(s) 1342
LmnI GCTCC 5 cut(s) 20, 145, 405, 865, 1159
Lsp1109I GCAGC 9 cut(s) 226, 329, 520, 815, 1261, 1498, 1561, 1726, 1727
LweI GCATC 8 cut(s) 294, 1022, 1561, 1638, 1639, 1751, 1815, 1903
MaeI CTAG 3 cut(s) 885, 1017, 1328
MaeII ACGT 3 cut(s) 196, 657, 975
MaeIII GTNAC 5 cut(s) 192, 197, 754, 950, 1421
MalI GATC 9 cut(s) 630, 741, 822, 989, 1083, 1254, 1532, 1554, 1920
MboI GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
MflI RGATCY 2 cut(s) 987, 1552
MhlI GDGCHC 2 cut(s) 17, 850
MlsI TGGCCA 1 cut(s) 1001
MluCI AATT 6 cut(s) 364, 434, 898, 1278, 1288, 1683
MluNI TGGCCA 1 cut(s) 1001
MlyI GAGTC 3 cut(s) 194, 375, 1199
MmeI TCCRAC 2 cut(s) 281, 1417
Mox20I TGGCCA 1 cut(s) 1001
Mph1103I ATGCAT 1 cut(s) 1653
MscI TGGCCA 1 cut(s) 1001
MseI TTAA 1 cut(s) 674
MslI CAYNNNNRTG 3 cut(s) 480, 1383, 1656
Msp20I TGGCCA 1 cut(s) 1001
MspA1I CMGCKG 2 cut(s) 295, 1511
MspI CCGG 2 cut(s) 187, 1841
MspR9I CCNGG 7 cut(s) 173, 188, 431, 1365, 1673, 1728, 1841
Mva1269I GAATGC 1 cut(s) 1651
MvaI CCWGG 5 cut(s) 173, 431, 1365, 1673, 1728
MvnI CGCG 5 cut(s) 374, 440, 589, 825, 1522
MwoI GCNNNNNNNGC 9 cut(s) 188, 508, 647, 1019, 1399, 1667, 1712, 1853, 1874
NciI CCSGG 2 cut(s) 188, 1841
NcoI CCATGG 3 cut(s) 99, 980, 1446
NdeI CATATG 1 cut(s) 396
NdeII GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
NlaIV GGNNCC 6 cut(s) 354, 640, 848, 849, 1838, 1839
NmuCI GTSAC 4 cut(s) 192, 197, 950, 1421
NruI TCGCGA 1 cut(s) 440
NsbI TGCGCA 1 cut(s) 1629
NsiI ATGCAT 1 cut(s) 1653
NspI RCATGY 4 cut(s) 479, 596, 1118, 1402
PaeI GCATGC 1 cut(s) 596
PaqCI CACCTGC 1 cut(s) 976
PceI AGGCCT 1 cut(s) 176
PciI ACATGT 2 cut(s) 475, 1114
PciSI GCTCTTC 1 cut(s) 1342
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 1651
PfeI GAWTC 6 cut(s) 1472, 1547, 1802, 1932, 2006, 2017
PflMI CCANNNNNTGG 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 429
PleI GAGTC 3 cut(s) 194, 375, 1198
PmaCI CACGTG 1 cut(s) 197
PmlI CACGTG 1 cut(s) 197
PpsI GAGTC 3 cut(s) 194, 375, 1198
Ppu21I YACGTR 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 1837
PscI ACATGT 2 cut(s) 475, 1114
Psp124BI GAGCTC 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 1837
Psp6I CCWGG 5 cut(s) 171, 429, 1363, 1671, 1726
PspCI CACGTG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 5 cut(s) 171, 429, 1363, 1671, 1726
PspN4I GGNNCC 6 cut(s) 354, 640, 848, 849, 1838, 1839
PspOMI GGGCCC 1 cut(s) 846
PspPI GGNCC 6 cut(s) 103, 352, 846, 847, 1439, 1837
PspPPI RGGWCCY 1 cut(s) 1837
PstNI CAGNNNCTG 1 cut(s) 868
PsuI RGATCY 2 cut(s) 987, 1552
PvuII CAGCTG 1 cut(s) 295
RruI TCGCGA 1 cut(s) 440
RsaI GTAC 1 cut(s) 1286
RsaNI GTAC 1 cut(s) 1285
RseI CAYNNNNRTG 3 cut(s) 480, 1383, 1656
SacI GAGCTC 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 1342
SaqAI TTAA 1 cut(s) 674
Sau3AI GATC 9 cut(s) 628, 739, 820, 987, 1081, 1252, 1530, 1552, 1918
Sau96I GGNCC 6 cut(s) 103, 352, 846, 847, 1439, 1837
SchI GAGTC 3 cut(s) 194, 375, 1199
ScrFI CCNGG 7 cut(s) 173, 188, 431, 1365, 1673, 1728, 1841
SduI GDGCHC 2 cut(s) 17, 850
SfaNI GCATC 8 cut(s) 294, 1022, 1561, 1638, 1639, 1751, 1815, 1903
SfcI CTRYAG 1 cut(s) 1988
SinI GGWCC 4 cut(s) 103, 352, 1439, 1837
SmiMI CAYNNNNRTG 3 cut(s) 480, 1383, 1656
SmlI CTYRAG 2 cut(s) 503, 1354
SmoI CTYRAG 2 cut(s) 503, 1354
SphI GCATGC 1 cut(s) 596
Sse9I AATT 6 cut(s) 364, 434, 898, 1278, 1288, 1683
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 9 cut(s) 30, 372, 589, 825, 1173, 1225, 1402, 1509, 1520
SspMI CTAG 3 cut(s) 885, 1017, 1328
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 7 cut(s) 171, 186, 429, 1363, 1671, 1726, 1839
StyI CCWWGG 4 cut(s) 99, 177, 980, 1446
TaaI ACNGT 6 cut(s) 419, 427, 463, 1209, 1375, 1852
TaiI ACGT 3 cut(s) 199, 660, 978
TaqI TCGA 2 cut(s) 471, 1633
TasI AATT 6 cut(s) 364, 434, 898, 1278, 1288, 1683
TatI WGTACW 1 cut(s) 1284
TauI GCSGC 1 cut(s) 592
TfiI GAWTC 6 cut(s) 1472, 1547, 1802, 1932, 2006, 2017
Tru1I TTAA 1 cut(s) 674
Tru9I TTAA 1 cut(s) 674
TscAI CASTG 5 cut(s) 229, 424, 430, 1009, 1779
TseFI GTSAC 4 cut(s) 192, 197, 950, 1421
TseI GCWGC 9 cut(s) 239, 317, 508, 803, 1274, 1511, 1574, 1715, 1739
Tsp45I GTSAC 4 cut(s) 192, 197, 950, 1421
TspDTI ATGAA 8 cut(s) 239, 1020, 1086, 1161, 1227, 1277, 1367, 1580
TspGWI ACGGA 1 cut(s) 1426
TspRI CASTG 5 cut(s) 229, 424, 430, 1009, 1779
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 4 cut(s) 103, 352, 1439, 1837
XapI RAATTY 2 cut(s) 434, 1683
XbaI TCTAGA 1 cut(s) 1016
XceI RCATGY 4 cut(s) 479, 596, 1118, 1402
XcmI CCANNNNNNNNNTGG 1 cut(s) 1453
XmiI GTMKAC 2 cut(s) 379, 519
XspI CTAG 3 cut(s) 885, 1017, 1328
Zsp2I ATGCAT 1 cut(s) 1653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.