pycom12g22410

Phosphoribosylaminoimidazole carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008753
Physical Location & Seq
Forward (+)
23296340 .. 23299239
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1254 bp
ATGATAAAAAGTAAAAGGTTAGCTTACGATGGACGTGGAAATGCTGTTGCTAAGAGTGAGGATGAGCTTTCGTCTGCTGTGACTGCTCTTGGAGGGTTTGATCGTGGCTTGTATGTTGAGAAATGGGCCCCATTTGTAAAGGAGCTGGCTGTTATTGTCGCAAGAGGAAGAGACAATTCTATCGTGTGCTACCCTGTTGTTGAAACAATACACAAAGAAAACATTTGTCACATTGTAAAGGCACCTGCTAACATGTCATGGAAGATCAGAAAGCTGGCCACTGATATTGCAACCAGAGCTGTTCGTTCATTAGAAGGTGCTGGTGTCTTTGCAGTTGAGTTGTTCTTAACAAAGGATGATCAGATTTTGCTAAATGAAGTAGCTCCTAGACCTCACAATAGCGGTCATCACACAATAGAGTCTTGCCACACTTCACAGTATGAACAGCATTTGAGGGCTGTTGTTGGTCTTCCACTTGGTGATCCATCGATGAAAACTCCAGCTGCGATTATGTACAATTTACTTGGTGAAGATGAGGGGGAACCTGGTTTCCTTCTAGCTCAGCAGCTGATTAGAAGGGCATTGCATATTCCAGGGGCCACTGTTCATTGGTATGATAAGCCAGAAATGCGGAAGCAGCGGAAGATGGGTCATATCACCATTGTTGGACCTTCCCTGGGCAATGTCGAAAAGCTTCTAGAGTCGATGCTAAATGAAGAAAGATTCGATAGGCAGTCTGCAGTCATACCGCGTGTTGGTATCGTAATGGGCTCTGATTCAGATCTTCCTGTTATGAAAGATGCTGCAAAGATTTTGAATACGTTTGGAGTACCTAATGAGGTGAGAATAGTTTCAGCACATCGAACTCCTGAATTGATGTATTCTTATGCCTTGTCTGCTCGGGAGAGAGGCATTCAGGTCATCATTGCTGGTGCTGGTGGTGCAGCGCACTTGCCAGGCATGGTAGCTGCCCTCACTCCTTTGCCTGTTATTGGTGTCCCTGTGCGCGCTTCTACATTGGATGGAATCGATTCTCTTTTATCCATTGTGCAGATGCCGAGAGGGGTCCCAGTTGCAACAGTAGCTGTAAACAACGCCACCAATGCTGGTTTGCTGGCAGTTAGGATATTGGGTGTTTGTGATGCTGATCTAGTATCAAGAATGACACAGTATCAAGAAGACACAAGGGACGAAGTTTTGACGAAGGCAGAGAAACTACAGAGAGATGGTTGGGAGTCTTATTTGAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 ko01110 map00230 map01100 map01110
Pfam Domains
Protein Families
AIRC ATP-grasp

Protein Analysis

418

Amino Acids

45.47

Weight (kDa)

6.88

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-grasp PF02222 1 - 147 8.6e-56 ATP-grasp domain
Dala_Dala_lig_C PF07478 15 - 131 2.9e-07 D-ala D-ala ligase C-terminus
PurK_C PF17769 169 - 230 1.4e-18 Phosphoribosylaminoimidazole carboxylase C-terminal domain
AIRC PF00731 251 - 397 1.8e-65 AIR carboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 241
AccII CGCG 2 cut(s) 751, 1008
AciI CCGC 4 cut(s) 402, 631, 640, 749
AclWI GGATC 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 2 cut(s) 515, 831
AfiI CCNNNNNNNGG 3 cut(s) 677, 755, 992
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 3 cut(s) 203, 817, 1246
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 4 cut(s) 544, 592, 675, 955
AloI GAACNNNNNNTCC 2 cut(s) 534, 566
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 1 cut(s) 476
AlwNI CAGNNNCTG 2 cut(s) 568, 1085
Ama87I CYCGRG 1 cut(s) 900
AoxI GGCC 3 cut(s) 126, 276, 597
ApaI GGGCCC 1 cut(s) 130
ApeKI GCWGC 6 cut(s) 503, 565, 637, 803, 944, 968
Asp700I GAANNNNTTC 3 cut(s) 693, 850, 1030
AspLEI GCGC 3 cut(s) 949, 1008, 1010
AspS9I GGNCC 5 cut(s) 126, 127, 597, 668, 1066
AsuHPI GGTGA 4 cut(s) 491, 539, 649, 853
AvaI CYCGRG 1 cut(s) 900
AvaII GGWCC 2 cut(s) 668, 1066
BaeGI GKGCMC 1 cut(s) 130
BalI TGGCCA 1 cut(s) 278
BanI GGYRCC 1 cut(s) 241
BanII GRGCYC 2 cut(s) 130, 773
BbsI GAAGAC 2 cut(s) 461, 1185
BbvI GCAGC 6 cut(s) 490, 577, 649, 790, 955, 956
BccI CCATC 5 cut(s) 23, 493, 640, 1016, 1220
BciT130I CCWGG 4 cut(s) 546, 594, 677, 957
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 4 cut(s) 387, 557, 698, 1151
BfmI CTRYAG 2 cut(s) 738, 1217
BfuAI ACCTGC 1 cut(s) 253
BglII AGATCT 1 cut(s) 781
BisI GCNGC 6 cut(s) 504, 566, 638, 804, 945, 969
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 6 cut(s) 505, 567, 639, 805, 946, 970
Bme1390I CCNGG 4 cut(s) 546, 594, 677, 957
Bme18I GGWCC 2 cut(s) 668, 1066
BmeT110I CYCGRG 1 cut(s) 900
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 5 cut(s) 126, 127, 597, 668, 1066
BmiI GGNNCC 7 cut(s) 128, 129, 243, 543, 598, 1067, 1068
BmrFI CCNGG 4 cut(s) 546, 594, 677, 957
BmrI ACTGGG 1 cut(s) 1064
BmsI GCATC 4 cut(s) 696, 790, 1044, 1132
BmuI ACTGGG 1 cut(s) 1064
BpiI GAAGAC 2 cut(s) 461, 1185
BpmI CTGGAG 1 cut(s) 483
Bpu1102I GCTNAGC 1 cut(s) 561
Bsa29I ATCGAT 2 cut(s) 488, 1029
BsaBI GATNNNNATC 2 cut(s) 780, 1146
BsaJI CCNNGG 3 cut(s) 593, 675, 676
Bsc4I CCNNNNNNNGG 3 cut(s) 677, 755, 992
Bse1I ACTGG 1 cut(s) 1070
Bse3DI GCAATG 3 cut(s) 581, 688, 924
Bse8I GATNNNNATC 2 cut(s) 780, 1146
BseBI CCWGG 4 cut(s) 546, 594, 677, 957
BseCI ATCGAT 2 cut(s) 488, 1029
BseDI CCNNGG 3 cut(s) 593, 675, 676
BseGI GGATG 3 cut(s) 67, 361, 1027
BseJI GATNNNNATC 2 cut(s) 780, 1146
BseLI CCNNNNNNNGG 3 cut(s) 677, 755, 992
BseMI GCAATG 3 cut(s) 581, 688, 924
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 1 cut(s) 1070
BsePI GCGCGC 1 cut(s) 1006
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 6 cut(s) 490, 577, 649, 790, 955, 956
BsgI GTGCAG 2 cut(s) 963, 1070
Bsh1236I CGCG 2 cut(s) 751, 1008
BshFI GGCC 3 cut(s) 128, 278, 599
BshNI GGYRCC 1 cut(s) 241
BshVI ATCGAT 2 cut(s) 488, 1029
BsiHKCI CYCGRG 1 cut(s) 900
BslFI GGGAC 3 cut(s) 983, 1052, 1202
BslI CCNNNNNNNGG 3 cut(s) 677, 755, 992
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 3 cut(s) 983, 1052, 1202
BsmI GAATGC 1 cut(s) 912
BsnI GGCC 3 cut(s) 128, 278, 599
BsoBI CYCGRG 1 cut(s) 900
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 130, 773
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
Bsp1720I GCTNAGC 1 cut(s) 561
BspACI CCGC 4 cut(s) 402, 631, 640, 749
BspANI GGCC 3 cut(s) 128, 278, 599
BspCNI CTCAG 1 cut(s) 574
BspDI ATCGAT 2 cut(s) 488, 1029
BspFNI CGCG 2 cut(s) 751, 1008
BspLI GGNNCC 7 cut(s) 128, 129, 243, 543, 598, 1067, 1068
BspMAI CTGCAG 1 cut(s) 742
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 1 cut(s) 476
BspT107I GGYRCC 1 cut(s) 241
BsrDI GCAATG 3 cut(s) 581, 688, 924
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 1 cut(s) 1070
BssECI CCNNGG 3 cut(s) 593, 675, 676
BssHII GCGCGC 1 cut(s) 1006
BssMI GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
Bst2UI CCWGG 4 cut(s) 546, 594, 677, 957
Bst4CI ACNGT 4 cut(s) 438, 604, 1081, 1170
Bst6I CTCTTC 1 cut(s) 163
BstAUI TGTACA 1 cut(s) 513
BstC8I GCNNGC 4 cut(s) 147, 276, 1008, 1116
BstDEI CTNAG 2 cut(s) 51, 561
BstF5I GGATG 3 cut(s) 67, 361, 1027
BstFNI CGCG 2 cut(s) 751, 1008
BstHHI GCGC 3 cut(s) 949, 1008, 1010
BstKTI GATC 6 cut(s) 103, 267, 361, 484, 784, 1150
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
BstMWI GCNNNNNNNGC 8 cut(s) 83, 296, 628, 637, 896, 941, 1082, 1103
BstNI CCWGG 4 cut(s) 546, 594, 677, 957
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 4 cut(s) 544, 592, 675, 955
BstSFI CTRYAG 2 cut(s) 738, 1217
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 2 cut(s) 751, 1008
BstV1I GCAGC 6 cut(s) 490, 577, 649, 790, 955, 956
BstV2I GAAGAC 2 cut(s) 461, 1185
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
Bsu15I ATCGAT 2 cut(s) 488, 1029
BsuRI GGCC 3 cut(s) 128, 278, 599
BsuTUI ATCGAT 2 cut(s) 488, 1029
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 3 cut(s) 67, 361, 1027
BtsIMutI CAGTG 2 cut(s) 279, 600
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 4 cut(s) 147, 276, 1008, 1116
CaiI CAGNNNCTG 2 cut(s) 568, 1085
CfoI GCGC 3 cut(s) 949, 1008, 1010
Cfr13I GGNCC 5 cut(s) 126, 127, 597, 668, 1066
ClaI ATCGAT 2 cut(s) 488, 1029
CsiI ACCWGGT 1 cut(s) 544
Csp6I GTAC 2 cut(s) 514, 830
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 3 cut(s) 253, 258, 961
CviQI GTAC 2 cut(s) 514, 830
DdeI CTNAG 2 cut(s) 51, 561
DpnI GATC 6 cut(s) 102, 266, 360, 483, 783, 1149
DpnII GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
DraIII CACNNNGTG 1 cut(s) 479
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 130, 773
Eco47I GGWCC 2 cut(s) 668, 1066
Eco88I CYCGRG 1 cut(s) 900
EcoO109I RGGNCCY 2 cut(s) 127, 1066
EcoRII CCWGG 4 cut(s) 544, 592, 675, 955
EcoT38I GRGCYC 2 cut(s) 130, 773
FaeI CATG 3 cut(s) 256, 261, 964
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 3 cut(s) 983, 1052, 1202
FatI CATG 3 cut(s) 252, 257, 960
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 6 cut(s) 504, 566, 638, 804, 945, 969
FokI GGATG 3 cut(s) 74, 368, 1034
FriOI GRGCYC 2 cut(s) 130, 773
Fsp4HI GCNGC 6 cut(s) 504, 566, 638, 804, 945, 969
FspBI CTAG 4 cut(s) 387, 557, 698, 1151
GlaI GCGC 3 cut(s) 948, 1007, 1009
GluI GCNGC 6 cut(s) 504, 566, 638, 804, 945, 969
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 3 cut(s) 128, 278, 599
HhaI GCGC 3 cut(s) 949, 1008, 1010
Hin1II CATG 3 cut(s) 256, 261, 964
Hin6I GCGC 3 cut(s) 947, 1006, 1008
HinP1I GCGC 3 cut(s) 947, 1006, 1008
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 8 cut(s) 419, 701, 723, 776, 1026, 1031, 1235, 1246
HphI GGTGA 4 cut(s) 491, 539, 649, 853
Hpy166II GTNNAC 1 cut(s) 1090
Hpy188I TCNGA 4 cut(s) 269, 363, 775, 781
Hpy188III TCNNGA 5 cut(s) 698, 869, 902, 1158, 1175
Hpy8I GTNNAC 1 cut(s) 1090
HpyAV CCTTC 5 cut(s) 308, 563, 570, 681, 1198
HpyCH4III ACNGT 4 cut(s) 438, 604, 1081, 1170
HpyCH4IV ACGT 2 cut(s) 34, 821
HpyCH4V TGCA 8 cut(s) 290, 332, 586, 740, 806, 944, 1051, 1076
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 296, 628, 637, 896, 941, 1082, 1103
HpyF3I CTNAG 2 cut(s) 51, 561
HpySE526I ACGT 2 cut(s) 34, 821
Hsp92II CATG 3 cut(s) 256, 261, 964
HspAI GCGC 3 cut(s) 947, 1006, 1008
KflI GGGWCCC 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
LmnI GCTCC 2 cut(s) 142, 388
Lsp1109I GCAGC 6 cut(s) 490, 577, 649, 790, 955, 956
LweI GCATC 4 cut(s) 696, 790, 1044, 1132
MabI ACCWGGT 1 cut(s) 544
MaeI CTAG 4 cut(s) 387, 557, 698, 1151
MaeII ACGT 2 cut(s) 34, 821
MaeIII GTNAC 2 cut(s) 79, 227
MalI GATC 6 cut(s) 102, 266, 360, 483, 783, 1149
MboI GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
MboII GAAGA 8 cut(s) 180, 274, 461, 542, 655, 728, 776, 1190
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 2 cut(s) 130, 773
MlsI TGGCCA 1 cut(s) 278
MluCI AATT 3 cut(s) 175, 517, 872
MluNI TGGCCA 1 cut(s) 278
MlyI GAGTC 3 cut(s) 428, 710, 1244
MmeI TCCRAC 1 cut(s) 646
Mox20I TGGCCA 1 cut(s) 278
MroXI GAANNNNTTC 3 cut(s) 693, 850, 1030
MscI TGGCCA 1 cut(s) 278
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 278
MspA1I CMGCKG 3 cut(s) 503, 568, 640
MspR9I CCNGG 4 cut(s) 546, 594, 677, 957
Mva1269I GAATGC 1 cut(s) 912
MvaI CCWGG 4 cut(s) 546, 594, 677, 957
MvnI CGCG 2 cut(s) 751, 1008
MwoI GCNNNNNNNGC 8 cut(s) 83, 296, 628, 637, 896, 941, 1082, 1103
NdeII GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
NlaIII CATG 3 cut(s) 256, 261, 964
NlaIV GGNNCC 7 cut(s) 128, 129, 243, 543, 598, 1067, 1068
NmeAIII GCCGAG 1 cut(s) 1083
NmuCI GTSAC 2 cut(s) 79, 227
NspI RCATGY 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 253
PasI CCCWGGG 1 cut(s) 676
PauI GCGCGC 1 cut(s) 1006
PciI ACATGT 1 cut(s) 252
PctI GAATGC 1 cut(s) 912
PdmI GAANNNNTTC 3 cut(s) 693, 850, 1030
PfeI GAWTC 5 cut(s) 723, 776, 1026, 1031, 1246
PkrI GCNGC 6 cut(s) 505, 567, 639, 805, 946, 970
PleI GAGTC 3 cut(s) 427, 709, 1243
PpsI GAGTC 3 cut(s) 427, 709, 1243
PpuMI RGGWCCY 1 cut(s) 1066
PscI ACATGT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 1066
Psp6I CCWGG 4 cut(s) 544, 592, 675, 955
PspGI CCWGG 4 cut(s) 544, 592, 675, 955
PspN4I GGNNCC 7 cut(s) 128, 129, 243, 543, 598, 1067, 1068
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 5 cut(s) 126, 127, 597, 668, 1066
PspPPI RGGWCCY 1 cut(s) 1066
PstI CTGCAG 1 cut(s) 742
PstNI CAGNNNCTG 2 cut(s) 568, 1085
PsuI RGATCY 1 cut(s) 781
PteI GCGCGC 1 cut(s) 1006
PvuII CAGCTG 2 cut(s) 503, 568
RsaI GTAC 2 cut(s) 515, 831
RsaNI GTAC 2 cut(s) 514, 830
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 6 cut(s) 504, 566, 638, 804, 945, 969
Sau3AI GATC 6 cut(s) 100, 264, 358, 481, 781, 1147
Sau96I GGNCC 5 cut(s) 126, 127, 597, 668, 1066
SchI GAGTC 3 cut(s) 428, 710, 1244
ScrFI CCNGG 4 cut(s) 546, 594, 677, 957
SduI GDGCHC 2 cut(s) 130, 773
SexAI ACCWGGT 1 cut(s) 544
SfaNI GCATC 4 cut(s) 696, 790, 1044, 1132
SfcI CTRYAG 2 cut(s) 738, 1217
SinI GGWCC 2 cut(s) 668, 1066
SmiMI CAYNNNNRTG 1 cut(s) 612
Sse9I AATT 3 cut(s) 175, 517, 872
SsiI CCGC 4 cut(s) 402, 631, 640, 749
SspMI CTAG 4 cut(s) 387, 557, 698, 1151
StyD4I CCNGG 4 cut(s) 544, 592, 675, 955
TaaI ACNGT 4 cut(s) 438, 604, 1081, 1170
TaiI ACGT 2 cut(s) 37, 824
TaqI TCGA 6 cut(s) 488, 687, 704, 726, 862, 1029
TasI AATT 3 cut(s) 175, 517, 872
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 5 cut(s) 723, 776, 1026, 1031, 1246
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TscAI CASTG 2 cut(s) 286, 607
TseFI GTSAC 2 cut(s) 79, 227
TseI GCWGC 6 cut(s) 503, 565, 637, 803, 944, 968
Tsp45I GTSAC 2 cut(s) 79, 227
TspDTI ATGAA 7 cut(s) 297, 390, 456, 506, 596, 729, 809
TspRI CASTG 2 cut(s) 286, 607
VpaK11BI GGWCC 2 cut(s) 668, 1066
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 1 cut(s) 256
XmnI GAANNNNTTC 3 cut(s) 693, 850, 1030
XspI CTAG 4 cut(s) 387, 557, 698, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.