pycom12g24100

Phospholipase A(1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005852
Physical Location & Seq
Forward (+)
24469663 .. 24474128
4466 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1359 bp
ATGCCAGGCCAGTGCTTCTGCCCCTGTTTCGACTTTGAACATGACAACCGCACCTCCTCTCAAACGGAGGTGGAGGTGGAGCCGGTGCTGCTGGTGTCGGGCATCGGAGGCTCCATTCTGCACTCCAGGAGGAGGAAGTTGGGCTTCGACACACGGGTCTGGGTCCGGATCTTATTGGCTGACTTGGAGTTCAAGAAGAAGCTCTGGTCTATCTACAATCCCCAAACAGGTTATACAGAAACGCTAGACAAAGAAACTGAAATTGTGGTCCCAGATGACGATTACGGCCTATATGCCATTGATATTCTAGATCCTTGCTGGTTTGTCAAATTGTTACGTTTGAAAGAGGTGTACCAGTTCCATGATATGATTGATATGCTTGTCGAGTGTGGGTATAAGAAGGGAACCACTTTGTTTGGATACGGATATGATTTTCGACAAAGCAATAGAATTGACAAGTCAATGGAGGGTCTTAAGGTGAAACTGGAAACTGCTTACAAGGCTTCTGGGGGTAGAAAAGTAAATATAATTTCGCATTCAATGGGTGGACTGCTAGTCACATGTTTTATGTCACTCCATACTGATGTATTTTCAAAGTACGTGAGTAAATGGATCTGCATAGCTTGCCCTTTTCAAGGTGCACCAGGATGCATCAATGATTCTCTCTTAACTGGATTACAGTTTGTTGAAGGCTTGGGAAATTACTTTTTTGTATCAAGGTGGACTATGCACCAGCTGTTGATTGAGTGTCCATCAATCTATGAGATGTTGGCAAATCCAAAATTTAATTGGAAAAGGCCGCCCGAAATTCAGGTTTGGAGGAACAAGTCTACGGATGGGGAAACCTTGGTTGATTTGGAGTCTTATGGCCCAATTGAAAGCATCCCTTTGTTTGAAGAAGCATTGAAATATAATGAGCTAAATTATGATGGTGAAACGGTAGCTTTGCCATTTAACTGTTCTATTCTCAGATGGGCTGCTGAGACTCGCCAAGTTTTGAACTGTGCTAAACTACCAGATAGCGTCAGCTTCTATAACGTATATGGCACATCGTTCGACACCCCTTTTGATGTTTGCTACGGTTCGGAGACAGCTCCAATTCAGGACCTGTCTGAAATATGCCATTCAATACCTCAGTATTCTTATGTGGATGGAGATGAAACAGTTCCGGCTGAGTCAGCAAAGGCGGATGGATTTGCTGCAGTGGAAAGAGTAGCAATAGCTGCAAGGCACCGTGAACTATTACGGGACAAAACAGTTTTTCAACATATCCTAAGGTGGTTGGGAGTCGAACAGAAGGTCAGCAGACATTCTAAGACATCAAGAGTTGCAGACGCCTCTTCAAATAAGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
LCAT

Protein Analysis

453

Amino Acids

51.41

Weight (kDa)

5.59

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 71 - 394 1.4e-23 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 1230
AccI GTMKAC 1 cut(s) 830
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 3 cut(s) 49, 800, 1187
AclWI GGATC 3 cut(s) 176, 305, 620
AcsI RAATTY 2 cut(s) 782, 807
AcyI GRCGYC 1 cut(s) 1335
AfaI GTAC 2 cut(s) 353, 599
AfiI CCNNNNNNNGG 3 cut(s) 132, 227, 635
AflII CTTAAG 1 cut(s) 473
AflIII ACRYGT 1 cut(s) 560
AhdI GACNNNNNGTC 1 cut(s) 554
AjnI CCWGG 3 cut(s) 4, 125, 643
AluBI AGCT 8 cut(s) 202, 623, 736, 919, 944, 1029, 1094, 1223
AluI AGCT 8 cut(s) 202, 623, 736, 919, 944, 1029, 1094, 1223
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 2 cut(s) 977, 1082
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 3 cut(s) 176, 305, 620
AlwNI CAGNNNCTG 1 cut(s) 1108
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 4 cut(s) 7, 286, 797, 868
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 4 cut(s) 88, 977, 1199, 1223
ApoI RAATTY 2 cut(s) 782, 807
ArsI GACNNNNNNTTYG 4 cut(s) 1049, 1081, 1284, 1316
Asp700I GAANNNNTTC 1 cut(s) 1164
AspS9I GGNCC 4 cut(s) 163, 268, 869, 1105
AsuHPI GGTGA 2 cut(s) 490, 944
AvaII GGWCC 3 cut(s) 163, 268, 1105
AxyI CCTNAGG 1 cut(s) 1274
BaeGI GKGCMC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 1230
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 4 cut(s) 75, 964, 1186, 1210
BccI CCATC 6 cut(s) 760, 830, 923, 966, 1145, 1184
BceAI ACGGC 1 cut(s) 301
BcgI CGANNNNNNTGC 2 cut(s) 1036, 1070
BciT130I CCWGG 3 cut(s) 6, 127, 645
BciVI GTATCC 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 977, 1082
BfaI CTAG 3 cut(s) 245, 308, 554
BfmI CTRYAG 1 cut(s) 1200
BfrI CTTAAG 1 cut(s) 473
BfuI GTATCC 1 cut(s) 413
BisI GCNGC 5 cut(s) 89, 800, 978, 1200, 1224
BlsI GCNGC 5 cut(s) 90, 801, 979, 1201, 1225
Bme1390I CCNGG 3 cut(s) 6, 127, 645
Bme18I GGWCC 3 cut(s) 163, 268, 1105
BmeRI GACNNNNNGTC 1 cut(s) 554
BmgT120I GGNCC 4 cut(s) 163, 268, 869, 1105
BmiI GGNNCC 6 cut(s) 81, 112, 164, 270, 406, 1232
BmrFI CCNGG 3 cut(s) 6, 127, 645
BmsI GCATC 4 cut(s) 111, 638, 660, 891
BpmI CTGGAG 1 cut(s) 109
BsaAI YACGTR 1 cut(s) 601
BsaHI GRCGYC 1 cut(s) 1335
BsaJI CCNNGG 1 cut(s) 846
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 227, 635
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 4 cut(s) 10, 355, 489, 676
Bse21I CCTNAGG 1 cut(s) 1274
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 3 cut(s) 6, 127, 645
BseDI CCNNGG 1 cut(s) 846
BseGI GGATG 5 cut(s) 653, 841, 882, 1156, 1195
BseLI CCNNNNNNNGG 3 cut(s) 132, 227, 635
BseMII CTCAG 4 cut(s) 972, 982, 1148, 1164
BseNI ACTGG 4 cut(s) 10, 355, 489, 676
BseRI GAGGAG 2 cut(s) 46, 145
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 4 cut(s) 75, 964, 1186, 1210
BsgI GTGCAG 1 cut(s) 104
BshFI GGCC 4 cut(s) 9, 288, 799, 870
BshNI GGYRCC 1 cut(s) 1230
BsiHKAI GWGCWC 1 cut(s) 643
BsiSI CCGG 3 cut(s) 83, 166, 1169
BslFI GGGAC 2 cut(s) 254, 1262
BslI CCNNNNNNNGG 3 cut(s) 132, 227, 635
BsmAI GTCTC 2 cut(s) 977, 1082
BsmFI GGGAC 2 cut(s) 254, 1262
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 4 cut(s) 9, 288, 799, 870
Bsp1286I GDGCHC 1 cut(s) 643
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 168, 310, 612
BspACI CCGC 3 cut(s) 49, 800, 1187
BspANI GGCC 4 cut(s) 9, 288, 799, 870
BspCNI CTCAG 4 cut(s) 973, 981, 1147, 1165
BspEI TCCGGA 1 cut(s) 165
BspLI GGNNCC 6 cut(s) 81, 112, 164, 270, 406, 1232
BspMAI CTGCAG 1 cut(s) 1204
BspPI GGATC 3 cut(s) 176, 305, 620
BspT107I GGYRCC 1 cut(s) 1230
BspTI CTTAAG 1 cut(s) 473
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 4 cut(s) 10, 355, 489, 676
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 846
BssMI GATC 3 cut(s) 168, 310, 612
BssNI GRCGYC 1 cut(s) 1335
BssT1I CCWWGG 1 cut(s) 846
Bst2UI CCWGG 3 cut(s) 6, 127, 645
Bst4CI ACNGT 8 cut(s) 681, 940, 959, 1004, 1082, 1165, 1235, 1258
Bst6I CTCTTC 1 cut(s) 1345
BstACI GRCGYC 1 cut(s) 1335
BstAFI CTTAAG 1 cut(s) 473
BstAPI GCANNNNNTGC 2 cut(s) 624, 1223
BstBAI YACGTR 1 cut(s) 601
BstC8I GCNNGC 2 cut(s) 625, 1353
BstDEI CTNAG 6 cut(s) 968, 981, 1134, 1173, 1274, 1314
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 5 cut(s) 653, 841, 882, 1156, 1195
BstKTI GATC 3 cut(s) 171, 313, 615
BstMAI GTCTC 2 cut(s) 977, 1082
BstMBI GATC 3 cut(s) 168, 310, 612
BstMWI GCNNNNNNNGC 6 cut(s) 88, 108, 500, 624, 1178, 1223
BstNI CCWGG 3 cut(s) 6, 127, 645
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 3 cut(s) 4, 125, 643
BstSFI CTRYAG 1 cut(s) 1200
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 4 cut(s) 75, 964, 1186, 1210
BstX2I RGATCY 3 cut(s) 168, 310, 612
BstYI RGATCY 3 cut(s) 168, 310, 612
Bsu36I CCTNAGG 1 cut(s) 1274
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 4 cut(s) 9, 288, 799, 870
BtsCI GGATG 5 cut(s) 653, 841, 882, 1156, 1195
BtsI GCAGTG 1 cut(s) 1209
BtsIMutI CAGTG 2 cut(s) 17, 1209
Cac8I GCNNGC 2 cut(s) 625, 1353
CaiI CAGNNNCTG 1 cut(s) 1108
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 4 cut(s) 163, 268, 869, 1105
CseI GACGC 2 cut(s) 1012, 1343
Csp6I GTAC 2 cut(s) 352, 598
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 3 cut(s) 41, 362, 561
CviQI GTAC 2 cut(s) 352, 598
DdeI CTNAG 6 cut(s) 968, 981, 1134, 1173, 1274, 1314
DpnI GATC 3 cut(s) 170, 312, 614
DpnII GATC 3 cut(s) 168, 310, 612
DrdI GACNNNNNNGTC 1 cut(s) 155
DriI GACNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 1345
Eam1105I GACNNNNNGTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 1345
EciI GGCGGA 1 cut(s) 1202
Eco130I CCWWGG 1 cut(s) 846
Eco47I GGWCC 3 cut(s) 163, 268, 1105
Eco81I CCTNAGG 1 cut(s) 1274
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 1105
EcoRII CCWGG 3 cut(s) 4, 125, 643
EcoT14I CCWWGG 1 cut(s) 846
EcoT22I ATGCAT 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 3 cut(s) 44, 365, 564
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 871, 903
FaqI GGGAC 2 cut(s) 254, 1262
FatI CATG 3 cut(s) 40, 361, 560
FblI GTMKAC 1 cut(s) 830
Fnu4HI GCNGC 5 cut(s) 89, 800, 978, 1200, 1224
FokI GGATG 5 cut(s) 660, 848, 869, 1163, 1202
Fsp4HI GCNGC 5 cut(s) 89, 800, 978, 1200, 1224
FspBI CTAG 3 cut(s) 245, 308, 554
GluI GCNGC 5 cut(s) 89, 800, 978, 1200, 1224
GsuI CTGGAG 1 cut(s) 109
HaeIII GGCC 4 cut(s) 9, 288, 799, 870
HapII CCGG 3 cut(s) 83, 166, 1169
HgaI GACGC 2 cut(s) 1012, 1343
Hin1I GRCGYC 1 cut(s) 1335
Hin1II CATG 3 cut(s) 44, 365, 564
HinfI GANTC 5 cut(s) 659, 860, 985, 1175, 1287
HpaII CCGG 3 cut(s) 83, 166, 1169
HphI GGTGA 2 cut(s) 490, 944
Hpy166II GTNNAC 6 cut(s) 352, 548, 641, 723, 831, 1238
Hpy188I TCNGA 4 cut(s) 107, 971, 1087, 1114
Hpy188III TCNNGA 5 cut(s) 166, 193, 308, 1103, 1323
Hpy8I GTNNAC 6 cut(s) 352, 548, 641, 723, 831, 1238
HpyAV CCTTC 3 cut(s) 394, 683, 1291
HpyCH4III ACNGT 8 cut(s) 681, 940, 959, 1004, 1082, 1165, 1235, 1258
HpyCH4IV ACGT 3 cut(s) 337, 600, 1038
HpyCH4V TGCA 8 cut(s) 121, 618, 641, 651, 730, 1202, 1226, 1331
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 108, 500, 624, 1178, 1223
HpyF3I CTNAG 6 cut(s) 968, 981, 1134, 1173, 1274, 1314
HpySE526I ACGT 3 cut(s) 337, 600, 1038
Hsp92I GRCGYC 1 cut(s) 1335
Hsp92II CATG 3 cut(s) 44, 365, 564
Kpn2I TCCGGA 1 cut(s) 165
Kzo9I GATC 3 cut(s) 168, 310, 612
LmnI GCTCC 3 cut(s) 79, 116, 1099
Lsp1109I GCAGC 4 cut(s) 75, 964, 1186, 1210
LweI GCATC 4 cut(s) 111, 638, 660, 891
MaeI CTAG 3 cut(s) 245, 308, 554
MaeII ACGT 3 cut(s) 337, 600, 1038
MaeIII GTNAC 3 cut(s) 333, 556, 570
MalI GATC 3 cut(s) 170, 312, 614
MboI GATC 3 cut(s) 168, 310, 612
MboII GAAGA 3 cut(s) 208, 908, 1332
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 3 cut(s) 168, 310, 612
MhlI GDGCHC 1 cut(s) 643
MlyI GAGTC 4 cut(s) 869, 979, 1184, 1296
Mph1103I ATGCAT 1 cut(s) 653
MroI TCCGGA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 4 cut(s) 474, 668, 786, 954
MslI CAYNNNNRTG 2 cut(s) 582, 646
MspA1I CMGCKG 1 cut(s) 736
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 3 cut(s) 83, 166, 1169
MspR9I CCNGG 3 cut(s) 6, 127, 645
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 3 cut(s) 6, 127, 645
MwoI GCNNNNNNNGC 6 cut(s) 88, 108, 500, 624, 1178, 1223
NdeII GATC 3 cut(s) 168, 310, 612
NlaIII CATG 3 cut(s) 44, 365, 564
NlaIV GGNNCC 6 cut(s) 81, 112, 164, 270, 406, 1232
NmuCI GTSAC 2 cut(s) 556, 570
NsiI ATGCAT 1 cut(s) 653
NspI RCATGY 1 cut(s) 564
PciI ACATGT 1 cut(s) 560
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 125
PkrI GCNGC 5 cut(s) 90, 801, 979, 1201, 1225
PleI GAGTC 4 cut(s) 868, 979, 1183, 1295
PpsI GAGTC 4 cut(s) 868, 979, 1183, 1295
Ppu21I YACGTR 1 cut(s) 601
PpuMI RGGWCCY 1 cut(s) 1105
PscI ACATGT 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 1105
Psp6I CCWGG 3 cut(s) 4, 125, 643
PspGI CCWGG 3 cut(s) 4, 125, 643
PspN4I GGNNCC 6 cut(s) 81, 112, 164, 270, 406, 1232
PspPI GGNCC 4 cut(s) 163, 268, 869, 1105
PspPPI RGGWCCY 1 cut(s) 1105
PstI CTGCAG 1 cut(s) 1204
PstNI CAGNNNCTG 1 cut(s) 1108
PsuI RGATCY 3 cut(s) 168, 310, 612
PvuII CAGCTG 1 cut(s) 736
RsaI GTAC 2 cut(s) 353, 599
RsaNI GTAC 2 cut(s) 352, 598
RseI CAYNNNNRTG 2 cut(s) 582, 646
SaqAI TTAA 4 cut(s) 474, 668, 786, 954
SatI GCNGC 5 cut(s) 89, 800, 978, 1200, 1224
Sau3AI GATC 3 cut(s) 168, 310, 612
Sau96I GGNCC 4 cut(s) 163, 268, 869, 1105
SchI GAGTC 4 cut(s) 869, 979, 1184, 1296
ScrFI CCNGG 3 cut(s) 6, 127, 645
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 4 cut(s) 111, 638, 660, 891
SfcI CTRYAG 1 cut(s) 1200
SinI GGWCC 3 cut(s) 163, 268, 1105
SmiMI CAYNNNNRTG 2 cut(s) 582, 646
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SsiI CCGC 3 cut(s) 49, 800, 1187
SspMI CTAG 3 cut(s) 245, 308, 554
StyD4I CCNGG 3 cut(s) 4, 125, 643
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 8 cut(s) 681, 940, 959, 1004, 1082, 1165, 1235, 1258
TaiI ACGT 3 cut(s) 340, 603, 1041
TaqI TCGA 6 cut(s) 30, 147, 384, 436, 1056, 1290
TauI GCSGC 1 cut(s) 802
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 4 cut(s) 474, 668, 786, 954
Tru9I TTAA 4 cut(s) 474, 668, 786, 954
TscAI CASTG 2 cut(s) 17, 1209
TseFI GTSAC 2 cut(s) 556, 570
TseI GCWGC 4 cut(s) 88, 977, 1199, 1223
Tsp45I GTSAC 2 cut(s) 556, 570
TspDTI ATGAA 1 cut(s) 1173
TspGWI ACGGA 3 cut(s) 80, 438, 848
TspRI CASTG 2 cut(s) 17, 1209
Vha464I CTTAAG 1 cut(s) 473
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 3 cut(s) 163, 268, 1105
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 782, 807
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 1 cut(s) 564
XcmI CCANNNNNNNNNTGG 1 cut(s) 786
XmiI GTMKAC 1 cut(s) 830
XmnI GAANNNNTTC 1 cut(s) 1164
XspI CTAG 3 cut(s) 245, 308, 554
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.