pycom13g00030

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005757
Physical Location & Seq
Reverse (-)
17122 .. 25582
8461 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2403 bp
ATGACTACTAAACGCGCTTACAAGCTACAGGAATTTGTAGCACATTCTGCAGCTGTGAATTGCCTCAAGATTGGCAGAAAATCTTCGAGAGTTCTTGTCACCGGCGGAGAAGATCACAAGGTCAATCTCTGGGCCATTGGGAAACCCAATGCCTTACTGAGTTTGTCTGGACACACCAGTGGAATTGATTCAGTAAGCTTTGATTCTTCAGAGGTCTTAGTAGCTGCAGGAGCTGCAAGTGGGACAATTAAGCTTTGGGATTTAGAGGAGGCAAAGATTGTTCGCACACTTACTGGTCACAGATCGAATTGTATATCTGTGGACTTTCATCCTTTTGGGGAGTTCTTTGCATCTGGCTCCTTAGACACAAATCTCAAAATATGGGATATCAGGAAGAAGGGGTGTATTCACACATACAAAGGCCATACACGAGGGGTCAATGCTATCAGATTTACCCCGGATGGCCGCTGGGTTGTTTCTTGTGGAGAGGACAACACTGTGAAGTTGTGGGACCTGACTGCTGGAAAGATGCTGCATGATTTCAAGTGTCATGAGGGCCAGGTTCAGTGCATAGACTTTCATCCCCATGAGTTTCTGCTAGCAACAGGTTCAGCAGATAGGACAGTCAAATTTTGGGACCTCGAAACCTTTGAGCTAATTGGCTCAGCTGGACCTGAGACAACTGGAGTGCGTTCTTTGACTTTTAGCCCGGATGGGAGAGTCCTACTCTGTGGATTGCATGAAAGTTTAAAGGTTTTCTCGTGGGAGCCAATAAGATGCCATGATGCTGTGGAGGTGGGATGGTCTCGATTGTCAGATCTTACTGTTCATGAGGGAAAGCTTCTTGGATGCTCATACAATCAGCGTTGTGTTGGAGTGTGGGGTGTAGACATCTCGCGCATAGAACCGTATGCAATTGGTAATGCCAGCAGATTGAGTGAGCACTCAGAATCTAAATCATTGTCTGGTGGGAATCTCTCCGTGCTCAATGAAAATGCTGCCAAATCTAGTTTGGGCCGGTTATCTATTTCTCAAAATTCAGATTCTTTAGTGAAGGAAACAAAGTCTCTTGGAAGGTTATCAGTTTCACAGAATTCAGATCTCGTTAAGGAGCCAAAAATTATTGCATCCACAGGAAGTTTTCCTGTTACTCCTCAAAGGATCAATTTGAATTCCAGTCCAAAAACTATTGCGGCTAGTTCAGTGGCAGTTCCTACTGTAACAACCCTGAAGAGGAGTTCTACTAAGACCCATTCAACAGCTAATATTCCAACATTTAACAAGTCTGATGTCATACCTGTAATTGTGCCAAGAACTAGTATGAGGTCAGAGCTGTCAGCTGAATCTAGAAAAGAGGTCGGCATTGCTGGAAGACCACTGGCGTTATCTTTGCAGTCAAAGGCAACTGATCTCCGGAAGTTGTCCAATAGCAGAGAAGAAGTGGATAAGCCAGCTGTGTCTGAATCCCCTGCTTCTAAGGCTATTGAATTAAGCAGTGCGGCAGATAAGAATGGCTTCTCAACAGTTATAAATTCAACTCGGGAAATACATGCTGAAAAAAACACAAAGGATGATATATGTTTTGCGCCTGGGAAGCTTGAAACAAGTTCAATGATGGAGCTGACTGCAAGTTACCAGCATGAAAGTTATGAGGCTCCAGGGCACAAGGTGATTAGAGATGCACATTTTGCTGAAGGTCAAAGAGGAGGGAGAATGCGCTCAATCGCTGTAAATTGGGAGAAAAGAGAGAGAAATTCTAACTACGAGGGACCTACTTCCAACGGTACCATGGGAACAGCATCTGCACCAAACATTATCCCTTTAATTGCGTTTAAGCAACGAGGTTATCCTACATCTACTGAAAAAGAAACAGTGACTGCTAGTGATGAGGATGCTATTGCAGATCTCCTGGAAAAACACAATCTATTTGTTGGCTCCATGCAATCTCGTCTTGCCAAATTACAGGTAGTCTATAGATATTGGGAAAGGAATGATGTTAAGGCAGTCCTTGGTGAAATGGAGAAGATGGCTGATCATGCTGTCATTGCAGACGTAGTTGGCATTATGACAGAAAAATTTGATATTGTCACGCTGGATATATGCACTTGTCTTCTGCCACTCCTCACTGGCCTTCTAGAAAGTAACATGGATAGGCATTTAGGCATTTCTTTGGACATGCTGCTCAAGTTGGTTAGAGTATTTGGTTCTGTGATCTATTCAGCAATATCTGCATCATCATCTGTTGGCGTTGATATTGAAGCCGAGAAAAGGCTGGAGCGTTGCAACATCTGTTTTATGGAGCTTGAAAAGGTCAAATGCAGTTTGCCTGCTCTTACCAGAAGAGGAGGATCAATTGCAAAGTCTGCACAGGAGTTGAGTTTAGCTCTTCAGGAAATTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

801

Amino Acids

87.24

Weight (kDa)

7.84

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 6 - 128 8.1e-08 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 6 - 172 5.1e-13 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 7 - 89 6.3e-09 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 130 5.9e-11 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 138 8.4e-26 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 130 5e-23 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 11 - 132 9.3e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 13 - 137 5.5e-13 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 15 - 255 7.8e-47 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 97 1.4e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 28 - 157 1.8e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_SCAP PF24017 28 - 129 6.6e-10 SCAP Beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 28 - 133 1.6e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 34 - 170 2.4e-13 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 50 - 250 3.3e-33 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 52 - 174 3.2e-18 WDR3 second beta-propeller domain
WD40 PF00400 52 - 87 3e-06 WD domain, G-beta repeat
EIF3I PF24805 53 - 139 8e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 54 - 138 5.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 57 - 177 1.5e-08 Echinoderm microtubule-associated protein first beta-propeller
WDR55 PF24796 62 - 258 4.4e-20 WDR55
Beta-prop_THOC3 PF25174 63 - 184 5.8e-29 THOC3 beta-propeller domain
Beta-prop_EIPR1 PF23609 67 - 170 1e-07 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 73 - 141 2.3e-12 WDHD1 first WD40 domain
WD40 PF00400 92 - 129 3.1e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 96 - 187 7.4e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 96 - 260 6.2e-38 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 102 - 190 1.1e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 114 - 253 5.6e-30 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 121 - 260 5.9e-17 G protein beta WD-40 repeat protein
Beta-prop_WDR75_1st PF23869 124 - 181 7e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 129 - 202 7.5e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 130 - 241 1.3e-07 MABP1/WDR62 first WD40 domain
WD40 PF00400 135 - 171 1.2e-13 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 137 - 218 7.6e-11 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 140 - 248 2.3e-17 EIF3I
ANAPC4_WD40 PF12894 142 - 194 6.4e-06 Anaphase-promoting complex subunit 4 WD40 domain
WD40_WDHD1_1st PF24817 144 - 254 1.2e-11 WDHD1 first WD40 domain
WDR90_beta-prop_4th PF23342 146 - 253 1.2e-07 WDR90, 4th beta-propeller
NBCH_WD40 PF20426 151 - 215 4.6e-06 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 153 - 249 4.1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 163 - 244 1.7e-11 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 164 - 246 1.1e-16 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 167 - 255 5e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd PF25172 169 - 218 1.9e-06 WDR3 second beta-propeller domain
WD40 PF00400 175 - 213 1.1e-09 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 178 - 262 1.6e-06 WDR36/Utp21 second beta-propeller domain
Katanin_con80 PF13925 639 - 796 1.3e-52 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1529
Acc65I GGTACC 1 cut(s) 1784
AccB1I GGYRCC 1 cut(s) 1784
AccI GTMKAC 1 cut(s) 888
AccII CGCG 2 cut(s) 15, 898
AccIII TCCGGA 1 cut(s) 1413
AciI CCGC 4 cut(s) 105, 466, 1193, 1499
AclWI GGATC 2 cut(s) 1169, 2356
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 9 cut(s) 32, 629, 1036, 1093, 1171, 1531, 1753, 2075, 2394
AcuI CTGAAG 4 cut(s) 192, 1250, 1713, 2372
AdeI CACNNNGTG 1 cut(s) 1669
AfaI GTAC 1 cut(s) 1786
AfiI CCNNNNNNNGG 2 cut(s) 1233, 2268
AgsI TTSAA 9 cut(s) 544, 1171, 1257, 1487, 1536, 1601, 1611, 2258, 2306
AhlI ACTAGT 1 cut(s) 1316
AjnI CCWGG 4 cut(s) 558, 1588, 1657, 1908
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 2 cut(s) 945, 987
Alw26I GTCTC 3 cut(s) 671, 810, 1071
AlwI GGATC 2 cut(s) 1169, 2356
AlwNI CAGNNNCTG 3 cut(s) 233, 1802, 1877
Ama87I CYCGRG 1 cut(s) 1539
Aor13HI TCCGGA 1 cut(s) 1413
AoxI GGCC 6 cut(s) 132, 421, 463, 556, 1015, 2128
ApeKI GCWGC 6 cut(s) 50, 224, 233, 532, 998, 2179
ApoI RAATTY 9 cut(s) 32, 629, 1036, 1093, 1171, 1531, 1753, 2075, 2394
Asp700I GAANNNNTTC 3 cut(s) 82, 187, 1514
Asp718I GGTACC 1 cut(s) 1784
AspLEI GCGC 4 cut(s) 17, 900, 1588, 1719
AspS9I GGNCC 7 cut(s) 132, 511, 556, 637, 671, 1015, 1769
AsuC2I CCSGG 2 cut(s) 458, 710
AsuHPI GGTGA 3 cut(s) 91, 1681, 2024
AsuNHI GCTAGC 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 1539
AvaII GGWCC 4 cut(s) 511, 637, 671, 1769
BaeGI GKGCMC 1 cut(s) 1665
BanI GGYRCC 1 cut(s) 1784
BauI CACGAG 2 cut(s) 429, 760
BbsI GAAGAC 2 cut(s) 1378, 2102
Bbv12I GWGCWC 2 cut(s) 945, 987
BbvI GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2166
BccI CCATC 5 cut(s) 455, 707, 795, 1609, 2020
BciT130I CCWGG 4 cut(s) 560, 1590, 1659, 1910
BclI TGATCA 1 cut(s) 2032
BcnI CCSGG 2 cut(s) 458, 710
BcoDI GTCTC 3 cut(s) 671, 810, 1071
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1881, 2135
BfmI CTRYAG 4 cut(s) 26, 48, 225, 1972
BglII AGATCT 3 cut(s) 817, 1099, 1903
BisI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1500, 2180
BlpI GCTNAGC 1 cut(s) 664
BlsI GCNGC 9 cut(s) 52, 226, 235, 467, 534, 1000, 1195, 1501, 2181
Bme1390I CCNGG 6 cut(s) 458, 560, 710, 1590, 1659, 1910
Bme18I GGWCC 4 cut(s) 511, 637, 671, 1769
BmeT110I CYCGRG 1 cut(s) 1539
BmgT120I GGNCC 7 cut(s) 132, 511, 556, 637, 671, 1015, 1769
BmiI GGNNCC 9 cut(s) 358, 512, 638, 768, 1113, 1656, 1770, 1786, 1936
BmrFI CCNGG 6 cut(s) 458, 560, 710, 1590, 1659, 1910
BmtI GCTAGC 1 cut(s) 602
BpiI GAAGAC 2 cut(s) 1378, 2102
BplI GAGNNNNNCTC 4 cut(s) 711, 743, 2368, 2400
BpmI CTGGAG 3 cut(s) 705, 1641, 2294
Bpu1102I GCTNAGC 1 cut(s) 664
BpuEI CTTGAG 2 cut(s) 50, 2168
BpuMI CCSGG 2 cut(s) 458, 710
BsaI GGTCTC 1 cut(s) 810
BsaJI CCNNGG 5 cut(s) 456, 1589, 1658, 1788, 2008
BsaWI WCCGGW 1 cut(s) 1413
Bsc4I CCNNNNNNNGG 2 cut(s) 1233, 2268
Bse118I RCCGGY 2 cut(s) 101, 1017
Bse1I ACTGG 6 cut(s) 177, 298, 688, 1176, 1383, 2131
Bse3DI GCAATG 2 cut(s) 1362, 2043
BseAI TCCGGA 1 cut(s) 1413
BseBI CCWGG 4 cut(s) 560, 1590, 1659, 1910
BseDI CCNNGG 5 cut(s) 456, 1589, 1658, 1788, 2008
BseGI GGATG 9 cut(s) 328, 466, 580, 718, 806, 854, 1127, 1576, 1897
BseLI CCNNNNNNNGG 2 cut(s) 1233, 2268
BseMI GCAATG 2 cut(s) 1362, 2043
BseMII CTCAG 4 cut(s) 149, 666, 678, 960
BseNI ACTGG 6 cut(s) 177, 298, 688, 1176, 1383, 2131
BseRI GAGGAG 6 cut(s) 281, 1143, 1249, 1719, 2111, 2358
BseSI GKGCMC 1 cut(s) 1665
BseXI GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2166
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 2 cut(s) 1788, 2349
Bsh1236I CGCG 2 cut(s) 15, 898
BshFI GGCC 6 cut(s) 134, 423, 465, 558, 1017, 2130
BshNI GGYRCC 1 cut(s) 1784
BsiHKAI GWGCWC 2 cut(s) 945, 987
BsiHKCI CYCGRG 1 cut(s) 1539
BsiSI CCGG 5 cut(s) 102, 458, 710, 1018, 1414
BslFI GGGAC 4 cut(s) 256, 524, 650, 1782
BslI CCNNNNNNNGG 2 cut(s) 1233, 2268
BsmAI GTCTC 3 cut(s) 671, 810, 1071
BsmFI GGGAC 4 cut(s) 256, 524, 650, 1782
BsmI GAATGC 1 cut(s) 1719
BsnI GGCC 6 cut(s) 134, 423, 465, 558, 1017, 2130
Bso31I GGTCTC 1 cut(s) 810
BsoBI CYCGRG 1 cut(s) 1539
Bsp1286I GDGCHC 3 cut(s) 945, 987, 1665
Bsp13I TCCGGA 1 cut(s) 1413
Bsp1720I GCTNAGC 1 cut(s) 664
Bsp19I CCATGG 1 cut(s) 1788
BspACI CCGC 4 cut(s) 105, 466, 1193, 1499
BspANI GGCC 6 cut(s) 134, 423, 465, 558, 1017, 2130
BspCNI CTCAG 4 cut(s) 150, 667, 677, 959
BspEI TCCGGA 1 cut(s) 1413
BspFNI CGCG 2 cut(s) 15, 898
BspHI TCATGA 3 cut(s) 550, 829, 2399
BspLI GGNNCC 9 cut(s) 358, 512, 638, 768, 1113, 1656, 1770, 1786, 1936
BspMAI CTGCAG 2 cut(s) 52, 229
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 2 cut(s) 1169, 2356
BspQI GCTCTTC 1 cut(s) 2391
BspT107I GGYRCC 1 cut(s) 1784
BspTNI GGTCTC 1 cut(s) 810
BsrDI GCAATG 2 cut(s) 1362, 2043
BsrFI RCCGGY 2 cut(s) 101, 1017
BsrI ACTGG 6 cut(s) 177, 298, 688, 1176, 1383, 2131
BssAI RCCGGY 2 cut(s) 101, 1017
BssECI CCNNGG 5 cut(s) 456, 1589, 1658, 1788, 2008
BssSI CACGAG 2 cut(s) 429, 760
BssT1I CCWWGG 2 cut(s) 1788, 2008
Bst2BI CACGAG 2 cut(s) 429, 760
Bst2UI CCWGG 4 cut(s) 560, 1590, 1659, 1910
Bst4CI ACNGT 8 cut(s) 499, 625, 826, 909, 1219, 1525, 1784, 1873
Bst6I CTCTTC 3 cut(s) 1226, 2335, 2391
BstAPI GCANNNNNTGC 5 cut(s) 47, 233, 1688, 2228, 2363
BstC8I GCNNGC 4 cut(s) 600, 928, 1452, 2328
BstDEI CTNAG 8 cut(s) 158, 217, 361, 664, 675, 946, 1245, 1476
BstDSI CCRYGG 1 cut(s) 1788
BstF5I GGATG 9 cut(s) 328, 466, 580, 718, 806, 854, 1127, 1576, 1897
BstFNI CGCG 2 cut(s) 15, 898
BstHHI GCGC 4 cut(s) 17, 900, 1588, 1719
BstMAI GTCTC 3 cut(s) 671, 810, 1071
BstNI CCWGG 4 cut(s) 560, 1590, 1659, 1910
BstNSI RCATGY 2 cut(s) 1553, 2179
BstSCI CCNGG 6 cut(s) 456, 558, 708, 1588, 1657, 1908
BstSFI CTRYAG 4 cut(s) 26, 48, 225, 1972
BstSLI GKGCMC 1 cut(s) 1665
BstUI CGCG 2 cut(s) 15, 898
BstV1I GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2166
BstV2I GAAGAC 2 cut(s) 1378, 2102
BstX2I RGATCY 3 cut(s) 817, 1099, 1903
BstYI RGATCY 3 cut(s) 817, 1099, 1903
BsuRI GGCC 6 cut(s) 134, 423, 465, 558, 1017, 2130
BtgI CCRYGG 1 cut(s) 1788
BtsCI GGATG 9 cut(s) 328, 466, 580, 718, 806, 854, 1127, 1576, 1897
BtsI GCAGTG 1 cut(s) 1501
BtsIMutI CAGTG 8 cut(s) 184, 495, 572, 1209, 1376, 1501, 1878, 2124
Cac8I GCNNGC 4 cut(s) 600, 928, 1452, 2328
CaiI CAGNNNCTG 3 cut(s) 233, 1802, 1877
CciI TCATGA 3 cut(s) 550, 829, 2399
CfoI GCGC 4 cut(s) 17, 900, 1588, 1719
Cfr10I RCCGGY 2 cut(s) 101, 1017
Cfr13I GGNCC 7 cut(s) 132, 511, 556, 637, 671, 1015, 1769
Csp6I GTAC 1 cut(s) 1785
CviQI GTAC 1 cut(s) 1785
DdeI CTNAG 8 cut(s) 158, 217, 361, 664, 675, 946, 1245, 1476
DraI TTTAAA 1 cut(s) 750
DraIII CACNNNGTG 1 cut(s) 1669
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 3 cut(s) 1226, 2335, 2391
EarI CTCTTC 3 cut(s) 1226, 2335, 2391
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 2 cut(s) 1788, 2008
Eco31I GGTCTC 1 cut(s) 810
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 4 cut(s) 511, 637, 671, 1769
Eco57I CTGAAG 4 cut(s) 192, 1250, 1713, 2372
Eco88I CYCGRG 1 cut(s) 1539
EcoO109I RGGNCCY 3 cut(s) 511, 637, 1769
EcoRI GAATTC 2 cut(s) 1093, 1171
EcoRII CCWGG 4 cut(s) 558, 1588, 1657, 1908
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 2 cut(s) 1788, 2008
ErhI CCWWGG 2 cut(s) 1788, 2008
FalI AAGNNNNNCTT 2 cut(s) 1499, 1531
FaqI GGGAC 4 cut(s) 256, 524, 650, 1782
FbaI TGATCA 1 cut(s) 2032
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1500, 2180
FokI GGATG 9 cut(s) 315, 473, 567, 725, 813, 861, 1114, 1583, 1904
Fsp4HI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1500, 2180
FspBI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1881, 2135
GlaI GCGC 4 cut(s) 16, 899, 1587, 1718
GluI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1500, 2180
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 3 cut(s) 705, 1641, 2294
HaeIII GGCC 6 cut(s) 134, 423, 465, 558, 1017, 2130
HapII CCGG 5 cut(s) 102, 458, 710, 1018, 1414
HhaI GCGC 4 cut(s) 17, 900, 1588, 1719
Hin6I GCGC 4 cut(s) 15, 898, 1586, 1717
HinP1I GCGC 4 cut(s) 15, 898, 1586, 1717
HindIII AAGCTT 4 cut(s) 196, 251, 839, 1595
HinfI GANTC 8 cut(s) 188, 203, 720, 950, 973, 1043, 1343, 1463
HpaII CCGG 5 cut(s) 102, 458, 710, 1018, 1414
HphI GGTGA 3 cut(s) 91, 1681, 2024
Hpy166II GTNNAC 2 cut(s) 322, 889
Hpy188I TCNGA 9 cut(s) 211, 449, 817, 949, 1042, 1099, 1288, 1330, 1462
Hpy8I GTNNAC 2 cut(s) 322, 889
HpyAV CCTTC 5 cut(s) 391, 1048, 1068, 1688, 2141
HpyCH4III ACNGT 8 cut(s) 499, 625, 826, 909, 1219, 1525, 1784, 1873
HpyCH4IV ACGT 1 cut(s) 2052
HpyF3I CTNAG 8 cut(s) 158, 217, 361, 664, 675, 946, 1245, 1476
HpySE526I ACGT 1 cut(s) 2052
HspAI GCGC 4 cut(s) 15, 898, 1586, 1717
Kpn2I TCCGGA 1 cut(s) 1413
KpnI GGTACC 1 cut(s) 1788
Ksp22I TGATCA 1 cut(s) 2032
LguI GCTCTTC 1 cut(s) 2391
LmnI GCTCC 9 cut(s) 230, 362, 766, 1111, 1618, 1660, 1940, 2275, 2299
Lsp1109I GCAGC 6 cut(s) 62, 211, 220, 519, 985, 2166
MaeI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1881, 2135
MaeII ACGT 1 cut(s) 2052
MaeIII GTNAC 8 cut(s) 97, 296, 1147, 1219, 1631, 1873, 2086, 2141
MfeI CAATTG 2 cut(s) 915, 2352
MflI RGATCY 3 cut(s) 817, 1099, 1903
MhlI GDGCHC 3 cut(s) 945, 987, 1665
MlyI GAGTC 1 cut(s) 729
MmeI TCCRAC 3 cut(s) 853, 1295, 1803
MroI TCCGGA 1 cut(s) 1413
MroXI GAANNNNTTC 3 cut(s) 82, 187, 1514
MseI TTAA 8 cut(s) 249, 749, 1107, 1278, 1490, 1823, 1833, 1998
MslI CAYNNNNRTG 2 cut(s) 177, 585
MspA1I CMGCKG 5 cut(s) 53, 468, 668, 1340, 1454
MspI CCGG 5 cut(s) 102, 458, 710, 1018, 1414
MspR9I CCNGG 6 cut(s) 458, 560, 710, 1590, 1659, 1910
MunI CAATTG 2 cut(s) 915, 2352
Mva1269I GAATGC 1 cut(s) 1719
MvaI CCWGG 4 cut(s) 560, 1590, 1659, 1910
MvnI CGCG 2 cut(s) 15, 898
NciI CCSGG 2 cut(s) 458, 710
NcoI CCATGG 1 cut(s) 1788
NheI GCTAGC 1 cut(s) 598
NlaIV GGNNCC 9 cut(s) 358, 512, 638, 768, 1113, 1656, 1770, 1786, 1936
NmeAIII GCCGAG 1 cut(s) 2287
NmuCI GTSAC 4 cut(s) 97, 296, 1873, 2086
NspI RCATGY 2 cut(s) 1553, 2179
OliI CACNNNNGTG 1 cut(s) 177
PagI TCATGA 3 cut(s) 550, 829, 2399
PciSI GCTCTTC 1 cut(s) 2391
PctI GAATGC 1 cut(s) 1719
PdmI GAANNNNTTC 3 cut(s) 82, 187, 1514
PfeI GAWTC 7 cut(s) 188, 203, 950, 973, 1043, 1343, 1463
PfoI TCCNGGA 1 cut(s) 1908
PkrI GCNGC 9 cut(s) 52, 226, 235, 467, 534, 1000, 1195, 1501, 2181
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
PpuMI RGGWCCY 3 cut(s) 511, 637, 1769
PsiI TTATAA 1 cut(s) 1529
Psp5II RGGWCCY 3 cut(s) 511, 637, 1769
Psp6I CCWGG 4 cut(s) 558, 1588, 1657, 1908
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 4 cut(s) 558, 1588, 1657, 1908
PspN4I GGNNCC 9 cut(s) 358, 512, 638, 768, 1113, 1656, 1770, 1786, 1936
PspPI GGNCC 7 cut(s) 132, 511, 556, 637, 671, 1015, 1769
PspPPI RGGWCCY 3 cut(s) 511, 637, 1769
PstI CTGCAG 2 cut(s) 52, 229
PstNI CAGNNNCTG 3 cut(s) 233, 1802, 1877
PsuI RGATCY 3 cut(s) 817, 1099, 1903
PvuII CAGCTG 4 cut(s) 53, 668, 1340, 1454
RsaI GTAC 1 cut(s) 1786
RsaNI GTAC 1 cut(s) 1785
RseI CAYNNNNRTG 2 cut(s) 177, 585
SapI GCTCTTC 1 cut(s) 2391
SaqAI TTAA 8 cut(s) 249, 749, 1107, 1278, 1490, 1823, 1833, 1998
SatI GCNGC 9 cut(s) 51, 225, 234, 466, 533, 999, 1194, 1500, 2180
Sau96I GGNCC 7 cut(s) 132, 511, 556, 637, 671, 1015, 1769
SchI GAGTC 1 cut(s) 729
ScrFI CCNGG 6 cut(s) 458, 560, 710, 1590, 1659, 1910
SduI GDGCHC 3 cut(s) 945, 987, 1665
SfcI CTRYAG 4 cut(s) 26, 48, 225, 1972
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 4 cut(s) 511, 637, 671, 1769
SmiMI CAYNNNNRTG 2 cut(s) 177, 585
SmlI CTYRAG 2 cut(s) 65, 2183
SmoI CTYRAG 2 cut(s) 65, 2183
SpeI ACTAGT 1 cut(s) 1316
SsiI CCGC 4 cut(s) 105, 466, 1193, 1499
SspI AATATT 1 cut(s) 1267
SspMI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1881, 2135
StyD4I CCNGG 6 cut(s) 456, 558, 708, 1588, 1657, 1908
StyI CCWWGG 2 cut(s) 1788, 2008
TaaI ACNGT 8 cut(s) 499, 625, 826, 909, 1219, 1525, 1784, 1873
TaiI ACGT 1 cut(s) 2055
TaqI TCGA 4 cut(s) 86, 305, 642, 808
TauI GCSGC 3 cut(s) 468, 1196, 1502
TfiI GAWTC 7 cut(s) 188, 203, 950, 973, 1043, 1343, 1463
Tru1I TTAA 8 cut(s) 249, 749, 1107, 1278, 1490, 1823, 1833, 1998
Tru9I TTAA 8 cut(s) 249, 749, 1107, 1278, 1490, 1823, 1833, 1998
TscAI CASTG 8 cut(s) 184, 502, 572, 1209, 1383, 1501, 1878, 2131
TseFI GTSAC 4 cut(s) 97, 296, 1873, 2086
TseI GCWGC 6 cut(s) 50, 224, 233, 532, 998, 2179
Tsp45I GTSAC 4 cut(s) 97, 296, 1873, 2086
TspDTI ATGAA 7 cut(s) 317, 569, 756, 818, 1005, 1656, 2388
TspGWI ACGGA 1 cut(s) 970
TspRI CASTG 8 cut(s) 184, 502, 572, 1209, 1383, 1501, 1878, 2131
VpaK11BI GGWCC 4 cut(s) 511, 637, 671, 1769
XapI RAATTY 9 cut(s) 32, 629, 1036, 1093, 1171, 1531, 1753, 2075, 2394
XbaI TCTAGA 2 cut(s) 1346, 2134
XceI RCATGY 2 cut(s) 1553, 2179
XcmI CCANNNNNNNNNTGG 2 cut(s) 1009, 1786
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 3 cut(s) 82, 187, 1514
XspI CTAG 7 cut(s) 599, 1008, 1197, 1317, 1347, 1881, 2135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.