pycom13g00450

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014077
Physical Location & Seq
Forward (+)
278261 .. 281226
2966 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGTATAGGTCCAGTGGTATTAACAAAACCCCCAACTCCTTAACCCGACCACATAGGAACCCGACCAACCCAGATCCTAAAACACAAAAACACAGTGCCTCGAACACTTCGAGGGTTCGAGTACCGTTTGAACCCATAAAACCTATCCATCGTCTGCGAATCCCTCCCCTCGTTTCTGTCATTATGAGAGGCTTAATCTCAAAGGCGAAGCTCCTGGCAAGCAGCAGGCCATGGCGCCCATCTCTGCGCAGCTACAGCTTGATCCCCATGGTCATTGAGCACTCCTCCCGAGGGGAACGCGCGTACGACATCTTCTCCCGCCTCCTCAAAGAGCCGATCATTTGCATCAACGGACCCATCGACGACGACACTTCCCACGTTGTCGTCGCGCAGCTTCTCTACTTGGAATCCGAGAACCCGTCGAAGCCCATCCACATGTACCTCAATTCCCCCGGTGGTCACGTCACTGCAGGTCTTGCAATTTATGATACGATGCAGTACATTCGGTCACCAATCAGTACCATTTGTTTGGGGCAAGCTGCATCAATGGCTTCTCTTCTCTTAGCTGCGGGCGCCAAGGGTGAGCGGCGGTCTCTCCCAAATGCTACAGTTATGATTCATCAGCCTTCAGGCGGGTACAGCGGACAGGCCAAAGATATGACAATTCACACGAAGCAGATTGTTCGTATCTGGGATTCCCTTAATGCGTTGTACTCAAAGCATACAGGGCAATCAGTAGATATAATAGAGAAGAATATGGACAGGGATCGATTTTATGACCCCAGAAGAGGCAAGGGCATTCGGAATCATTGA

Protein Analysis

271

Amino Acids

30.18

Weight (kDa)

10.13

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 97 - 261 1.2e-70 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 461
AccB1I GGYRCC 2 cut(s) 234, 572
AccBSI CCGCTC 1 cut(s) 586
AccII CGCG 3 cut(s) 300, 302, 389
AciI CCGC 6 cut(s) 319, 569, 586, 589, 633, 642
AclWI GGATC 3 cut(s) 68, 256, 774
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 2 cut(s) 235, 573
AfaI GTAC 7 cut(s) 123, 305, 440, 500, 520, 638, 713
AfiI CCNNNNNNNGG 3 cut(s) 291, 632, 788
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 1 cut(s) 131
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 6 cut(s) 211, 252, 258, 394, 539, 566
AluI AGCT 6 cut(s) 211, 252, 258, 394, 539, 566
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 3 cut(s) 68, 256, 774
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 2 cut(s) 227, 648
ApeKI GCWGC 5 cut(s) 222, 249, 391, 539, 566
AspLEI GCGC 5 cut(s) 237, 249, 302, 391, 575
AspS9I GGNCC 2 cut(s) 9, 353
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 501, 593
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 2 cut(s) 9, 353
BanI GGYRCC 2 cut(s) 234, 572
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 5 cut(s) 234, 261, 403, 526, 553
BccI CCATC 4 cut(s) 156, 247, 365, 437
BcgI CGANNNNNNTGC 4 cut(s) 485, 519, 665, 699
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 597
BfmI CTRYAG 3 cut(s) 253, 468, 606
BfoI RGCGCY 2 cut(s) 238, 576
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 6 cut(s) 223, 250, 392, 540, 567, 587
BlsI GCNGC 6 cut(s) 224, 251, 393, 541, 568, 588
Bme1390I CCNGG 2 cut(s) 215, 453
Bme18I GGWCC 2 cut(s) 9, 353
BmeT110I CYCGRG 1 cut(s) 288
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 2 cut(s) 9, 353
BmiI GGNNCC 4 cut(s) 59, 236, 355, 574
BmrFI CCNGG 2 cut(s) 215, 453
BmsI GCATC 3 cut(s) 354, 483, 551
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BpuMI CCSGG 1 cut(s) 453
Bsa29I ATCGAT 1 cut(s) 769
BsaHI GRCGYC 2 cut(s) 235, 573
BsaI GGTCTC 1 cut(s) 597
BsaJI CCNNGG 5 cut(s) 230, 267, 289, 451, 576
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 632, 788
Bse1I ACTGG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 769
BseDI CCNNGG 5 cut(s) 230, 267, 289, 451, 576
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 291, 632, 788
BseNI ACTGG 1 cut(s) 12
BseRI GAGGAG 2 cut(s) 274, 314
BseXI GCAGC 5 cut(s) 234, 261, 403, 526, 553
Bsh1236I CGCG 3 cut(s) 300, 302, 389
BshFI GGCC 2 cut(s) 229, 650
BshNI GGYRCC 2 cut(s) 234, 572
BshVI ATCGAT 1 cut(s) 769
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 1 cut(s) 453
BsiWI CGTACG 1 cut(s) 303
BslI CCNNNNNNNGG 3 cut(s) 291, 632, 788
BsmAI GTCTC 1 cut(s) 597
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 2 cut(s) 229, 650
Bso31I GGTCTC 1 cut(s) 597
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 4 cut(s) 73, 261, 336, 766
Bsp19I CCATGG 2 cut(s) 230, 267
BspACI CCGC 6 cut(s) 319, 569, 586, 589, 633, 642
BspANI GGCC 2 cut(s) 229, 650
BspDI ATCGAT 1 cut(s) 769
BspFNI CGCG 3 cut(s) 300, 302, 389
BspLI GGNNCC 4 cut(s) 59, 236, 355, 574
BspMAI CTGCAG 1 cut(s) 472
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 68, 256, 774
BspT107I GGYRCC 2 cut(s) 234, 572
BspTNI GGTCTC 1 cut(s) 597
BsrBI CCGCTC 1 cut(s) 586
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 5 cut(s) 230, 267, 289, 451, 576
BssMI GATC 4 cut(s) 73, 261, 336, 766
BssNI GRCGYC 2 cut(s) 235, 573
BssT1I CCWWGG 3 cut(s) 230, 267, 576
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 3 cut(s) 95, 126, 610
Bst6I CTCTTC 2 cut(s) 561, 781
BstACI GRCGYC 2 cut(s) 235, 573
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 4 cut(s) 220, 227, 537, 571
BstDEI CTNAG 1 cut(s) 562
BstDSI CCRYGG 2 cut(s) 230, 267
BstEII GGTNACC 1 cut(s) 507
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 3 cut(s) 300, 302, 389
BstH2I RGCGCY 2 cut(s) 238, 576
BstHHI GCGC 5 cut(s) 237, 249, 302, 391, 575
BstKTI GATC 4 cut(s) 76, 264, 339, 769
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 4 cut(s) 73, 261, 336, 766
BstMWI GCNNNNNNNGC 6 cut(s) 255, 476, 548, 572, 639, 727
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 439
BstPI GGTNACC 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 213, 451
BstSFI CTRYAG 3 cut(s) 253, 468, 606
BstUI CGCG 3 cut(s) 300, 302, 389
BstV1I GCAGC 5 cut(s) 234, 261, 403, 526, 553
BstX2I RGATCY 1 cut(s) 73
BstXI CCANNNNNNTGG 1 cut(s) 529
BstYI RGATCY 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 769
BsuRI GGCC 2 cut(s) 229, 650
BsuTUI ATCGAT 1 cut(s) 769
BtgI CCRYGG 2 cut(s) 230, 267
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 1 cut(s) 429
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 3 cut(s) 19, 100, 465
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 4 cut(s) 220, 227, 537, 571
CfoI GCGC 5 cut(s) 237, 249, 302, 391, 575
Cfr13I GGNCC 2 cut(s) 9, 353
ClaI ATCGAT 1 cut(s) 769
Csp6I GTAC 7 cut(s) 122, 304, 439, 499, 519, 637, 712
CviAII CATG 3 cut(s) 231, 268, 436
CviQI GTAC 7 cut(s) 122, 304, 439, 499, 519, 637, 712
DdeI CTNAG 1 cut(s) 562
DinI GGCGCC 2 cut(s) 236, 574
DpnI GATC 4 cut(s) 75, 263, 338, 768
DpnII GATC 4 cut(s) 73, 261, 336, 766
Eam1104I CTCTTC 2 cut(s) 561, 781
EarI CTCTTC 2 cut(s) 561, 781
Eco130I CCWWGG 3 cut(s) 230, 267, 576
Eco31I GGTCTC 1 cut(s) 597
Eco47I GGWCC 2 cut(s) 9, 353
Eco57I CTGAAG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 288
Eco91I GGTNACC 1 cut(s) 507
EcoO65I GGTNACC 1 cut(s) 507
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 3 cut(s) 230, 267, 576
EgeI GGCGCC 2 cut(s) 236, 574
EheI GGCGCC 2 cut(s) 236, 574
ErhI CCWWGG 3 cut(s) 230, 267, 576
FaeI CATG 3 cut(s) 234, 271, 439
FatI CATG 3 cut(s) 230, 267, 435
FauI CCCGC 3 cut(s) 326, 562, 626
Fnu4HI GCNGC 6 cut(s) 223, 250, 392, 540, 567, 587
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 6 cut(s) 223, 250, 392, 540, 567, 587
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 5 cut(s) 236, 248, 301, 390, 574
GluI GCNGC 6 cut(s) 223, 250, 392, 540, 567, 587
HaeII RGCGCY 2 cut(s) 238, 576
HaeIII GGCC 2 cut(s) 229, 650
HapII CCGG 1 cut(s) 453
HhaI GCGC 5 cut(s) 237, 249, 302, 391, 575
Hin1I GRCGYC 2 cut(s) 235, 573
Hin1II CATG 3 cut(s) 234, 271, 439
Hin6I GCGC 5 cut(s) 235, 247, 300, 389, 573
HinP1I GCGC 5 cut(s) 235, 247, 300, 389, 573
HinfI GANTC 5 cut(s) 159, 407, 616, 695, 805
HpaII CCGG 1 cut(s) 453
HphI GGTGA 2 cut(s) 501, 593
Hpy188I TCNGA 2 cut(s) 412, 804
Hpy188III TCNNGA 1 cut(s) 288
Hpy99I CGWCG 4 cut(s) 365, 368, 389, 424
HpyAV CCTTC 1 cut(s) 636
HpyCH4III ACNGT 3 cut(s) 95, 126, 610
HpyCH4IV ACGT 2 cut(s) 378, 462
HpyCH4V TGCA 5 cut(s) 345, 470, 479, 496, 542
HpyF10VI GCNNNNNNNGC 6 cut(s) 255, 476, 548, 572, 639, 727
HpyF3I CTNAG 1 cut(s) 562
HpySE526I ACGT 2 cut(s) 378, 462
Hsp92I GRCGYC 2 cut(s) 235, 573
Hsp92II CATG 3 cut(s) 234, 271, 439
HspAI GCGC 5 cut(s) 235, 247, 300, 389, 573
KasI GGCGCC 2 cut(s) 234, 572
Kzo9I GATC 4 cut(s) 73, 261, 336, 766
LmnI GCTCC 1 cut(s) 216
Lsp1109I GCAGC 5 cut(s) 234, 261, 403, 526, 553
LweI GCATC 3 cut(s) 354, 483, 551
MaeII ACGT 2 cut(s) 378, 462
MaeIII GTNAC 3 cut(s) 458, 463, 507
MalI GATC 4 cut(s) 75, 263, 338, 768
MbiI CCGCTC 1 cut(s) 586
MboI GATC 4 cut(s) 73, 261, 336, 766
MboII GAAGA 4 cut(s) 304, 548, 763, 798
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 3 cut(s) 445, 480, 663
Mly113I GGCGCC 2 cut(s) 235, 573
MseI TTAA 4 cut(s) 21, 41, 194, 702
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 642
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 2 cut(s) 215, 453
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 3 cut(s) 300, 302, 389
MwoI GCNNNNNNNGC 6 cut(s) 255, 476, 548, 572, 639, 727
NarI GGCGCC 2 cut(s) 235, 573
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 2 cut(s) 230, 267
NdeII GATC 4 cut(s) 73, 261, 336, 766
NlaIII CATG 3 cut(s) 234, 271, 439
NlaIV GGNNCC 4 cut(s) 59, 236, 355, 574
NmuCI GTSAC 3 cut(s) 458, 463, 507
NsbI TGCGCA 1 cut(s) 248
NspI RCATGY 1 cut(s) 439
PciI ACATGT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 357
PctI GAATGC 1 cut(s) 798
PfeI GAWTC 5 cut(s) 159, 407, 616, 695, 805
Pfl23II CGTACG 1 cut(s) 303
PkrI GCNGC 6 cut(s) 224, 251, 393, 541, 568, 588
PluTI GGCGCC 2 cut(s) 238, 576
PscI ACATGT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 213
PspEI GGTNACC 1 cut(s) 507
PspGI CCWGG 1 cut(s) 213
PspLI CGTACG 1 cut(s) 303
PspN4I GGNNCC 4 cut(s) 59, 236, 355, 574
PspPI GGNCC 2 cut(s) 9, 353
PstI CTGCAG 1 cut(s) 472
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 7 cut(s) 123, 305, 440, 500, 520, 638, 713
RsaNI GTAC 7 cut(s) 122, 304, 439, 499, 519, 637, 712
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 4 cut(s) 21, 41, 194, 702
SatI GCNGC 6 cut(s) 223, 250, 392, 540, 567, 587
Sau3AI GATC 4 cut(s) 73, 261, 336, 766
Sau96I GGNCC 2 cut(s) 9, 353
ScrFI CCNGG 2 cut(s) 215, 453
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 3 cut(s) 354, 483, 551
SfcI CTRYAG 3 cut(s) 253, 468, 606
SfoI GGCGCC 2 cut(s) 236, 574
SinI GGWCC 2 cut(s) 9, 353
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 3 cut(s) 445, 480, 663
SsiI CCGC 6 cut(s) 319, 569, 586, 589, 633, 642
SspDI GGCGCC 2 cut(s) 234, 572
StyD4I CCNGG 2 cut(s) 213, 451
StyI CCWWGG 3 cut(s) 230, 267, 576
TaaI ACNGT 3 cut(s) 95, 126, 610
TaiI ACGT 2 cut(s) 381, 465
TaqI TCGA 6 cut(s) 101, 110, 118, 360, 422, 769
TaqII GACCGA 1 cut(s) 495
TasI AATT 3 cut(s) 445, 480, 663
TatI WGTACW 2 cut(s) 498, 711
TauI GCSGC 1 cut(s) 589
TfiI GAWTC 5 cut(s) 159, 407, 616, 695, 805
Tru1I TTAA 4 cut(s) 21, 41, 194, 702
Tru9I TTAA 4 cut(s) 21, 41, 194, 702
TscAI CASTG 3 cut(s) 19, 100, 472
TseFI GTSAC 3 cut(s) 458, 463, 507
TseI GCWGC 5 cut(s) 222, 249, 391, 539, 566
Tsp45I GTSAC 3 cut(s) 458, 463, 507
TspDTI ATGAA 1 cut(s) 608
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 3 cut(s) 19, 100, 472
VpaK11BI GGWCC 2 cut(s) 9, 353
XceI RCATGY 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.