pycom13g03960

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007935
Physical Location & Seq
Reverse (-)
2612894 .. 2615676
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1503 bp
ATGAACAACATCTCTCACAGCTCTTCCTCCTCCTCCACCTCCTCCTCCATCGACACCGCTGATCCGAATCTAGTTTCGAGTCGGAGGAGCGTGGATGGTGGTCAAGGCTTGGCTGCTGAGCCATCGCGTCGATGGCGTGACATTTTCTGGTTGGGAGTGTTTCTGATTCATTTGATTTTGGTGGGATGTGCTCTTACGGTTCTGGGGACCAATAGGTTTAAGAAAACAGATAGGCTAAACATAGATAGGTACACCCAGAGATTTTTGGAAAATCAAAGGGGGTTGACGGAGAACTATTGGCTGCTTTATGCTCTTGCCGGGGGAGTTGGGACACTTCTTGGTTGGACATGGTTGTTTTTCCTCCGTTCTCAAGCAACTCACATGATGAAGGTTGCGGTTCACATCTTGACTACTTATCTTGCTGTGATTAGTGTTCTATTGTTTTGGATGGAACAAATTTTCTGGGGTGTTGCATTTGCGATTGGTGCTGTATTGCAGTTCATGTATGTTCTTTCAATTGTGGGCAGACTTCCCTTTACCATGCTGGTGCTGCAAGCAGCTGTCAAGATGGTATGGAGTCTTCGTGAAGTTATGAGGGTAGCGTATGCTTTCATGCTTATTATGCTTTTATGGATGGTATTATGGTCCTTCGGGGCAGCTGGTGTTGTAGCTTCAAGCATTGGAGATGGTGGACGCTGGTGGCTTCTAGTGGTTCTCTCTATAAGCTTATTTTGGACGGGTGCAGTACTCTGTAATACTGTGCATGTTATAGTGTCTGGGATGGTGTTTCTTGTTCTTATCCATGGTGGTAGGGAGGCACCATCAATGCCTCCAAACTCACTGATGAAGTCTCTTCGGTATGCAGTCACAACTTCTTTTGGCAGTATCTGCTATGGTTCACTTTTCACAGCTTTCATTCGGACACTGCGGTGGAAGATCAGGGGAGTCCGGTCCAAGATAGGCAACAACGAGTGCCTACTTTGCTGTGTTGATTTTCTCTTTCATCTAGTGGAGACTCTTGTCCGCTTCTTTAACAAGTATGCCTATGTGCAGATAGCTGTTTCTGGCAAAGGCTTCAATCATTCAGCGAGGGATGCCTGGGAGTTATTTCAGTCTACTGGAGTGGAGGCTCTTGTTGCCTACGATTGTTCAGGTGCTGTTTTATTGATGGGAACAGTTTTGGGCGGGCTTATTACTGGAACTTCCGCGGGGATCTGGACATGGATTAAGTATAGGGACAGAGTAGTTATGGTGGGGTCCACTGCAATGTTGATGGGAATGGTCTTGGTGGGGCTGGCGATGGCGGTTGTGGAAAGTGCCGTTACATCTATTTATATTTGTTATGCAGAAGACCCCTTGTTGATTCGCAGGTGGGATGCTGAGTTCTTCAACCAGATGTCGGAGATGCTACACCAGCGTCTTCAACATAGGAGCTCACGAGTGAGGGAAGTGTTCACCCACCACCACTTTGATAACCACACACAAGAAACACTCCCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

501

Amino Acids

56.1

Weight (kDa)

9.35

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 158 - 452 3.8e-58 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1359
Acc36I ACCTGC 1 cut(s) 1359
AccB1I GGYRCC 1 cut(s) 817
AccI GTMKAC 1 cut(s) 1115
AccII CGCG 2 cut(s) 127, 1208
AciI CCGC 8 cut(s) 57, 395, 928, 1024, 1185, 1206, 1208, 1304
AclWI GGATC 2 cut(s) 56, 1220
AcsI RAATTY 1 cut(s) 456
AfaI GTAC 2 cut(s) 251, 747
AfiI CCNNNNNNNGG 1 cut(s) 1399
AgsI TTSAA 5 cut(s) 516, 675, 1078, 1390, 1424
AjnI CCWGG 1 cut(s) 1097
AleI CACNNNNGTG 1 cut(s) 928
AloI GAACNNNNNNTCC 2 cut(s) 1367, 1399
AluBI AGCT 8 cut(s) 21, 560, 659, 671, 726, 911, 1058, 1434
AluI AGCT 8 cut(s) 21, 560, 659, 671, 726, 911, 1058, 1434
Alw21I GWGCWC 2 cut(s) 193, 1436
Alw26I GTCTC 2 cut(s) 855, 1007
AlwI GGATC 2 cut(s) 56, 1220
AlwNI CAGNNNCTG 2 cut(s) 888, 1157
ApeKI GCWGC 5 cut(s) 113, 301, 550, 557, 656
ApoI RAATTY 1 cut(s) 456
AspS9I GGNCC 4 cut(s) 207, 645, 951, 1257
AsuC2I CCSGG 1 cut(s) 319
AsuHPI GGTGA 1 cut(s) 1447
AvaII GGWCC 4 cut(s) 207, 645, 951, 1257
BanI GGYRCC 1 cut(s) 817
BanII GRGCYC 1 cut(s) 1436
BarI GAAGNNNNNNTAC 2 cut(s) 564, 596
BauI CACGAG 1 cut(s) 1437
BbsI GAAGAC 3 cut(s) 572, 1356, 1412
Bbv12I GWGCWC 2 cut(s) 193, 1436
BbvI GCAGC 5 cut(s) 100, 288, 537, 569, 668
BceAI ACGGC 1 cut(s) 1304
BciT130I CCWGG 1 cut(s) 1099
BcnI CCSGG 1 cut(s) 319
BcoDI GTCTC 2 cut(s) 855, 1007
BfaI CTAG 3 cut(s) 71, 707, 1007
BfuAI ACCTGC 1 cut(s) 1359
BisI GCNGC 5 cut(s) 114, 302, 551, 558, 657
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 5 cut(s) 115, 303, 552, 559, 658
BmcAI AGTACT 1 cut(s) 747
Bme1390I CCNGG 2 cut(s) 319, 1099
Bme18I GGWCC 4 cut(s) 207, 645, 951, 1257
BmgT120I GGNCC 4 cut(s) 207, 645, 951, 1257
BmiI GGNNCC 3 cut(s) 208, 819, 1258
BmrFI CCNGG 2 cut(s) 319, 1099
BmsI GCATC 3 cut(s) 1084, 1366, 1395
BoxI GACNNNNGTC 1 cut(s) 1019
BpiI GAAGAC 3 cut(s) 572, 1356, 1412
BpmI CTGGAG 1 cut(s) 1140
Bpu1102I GCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 354
BpuMI CCSGG 1 cut(s) 319
BsaBI GATNNNNATC 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 318, 802, 1098, 1206
BsaWI WCCGGW 1 cut(s) 948
BsaXI ACNNNNNCTCC 2 cut(s) 568, 598
Bsc4I CCNNNNNNNGG 1 cut(s) 1399
Bse1I ACTGG 2 cut(s) 1123, 1201
Bse3DI GCAATG 1 cut(s) 1272
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 1 cut(s) 1099
BseDI CCNNGG 4 cut(s) 318, 802, 1098, 1206
BseGI GGATG 7 cut(s) 100, 191, 453, 639, 786, 1099, 1381
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 1 cut(s) 1399
BseMI GCAATG 1 cut(s) 1272
BseMII CTCAG 2 cut(s) 108, 1371
BseNI ACTGG 2 cut(s) 1123, 1201
BseRI GAGGAG 5 cut(s) 19, 22, 31, 34, 100
BseXI GCAGC 5 cut(s) 100, 288, 537, 569, 668
BsgI GTGCAG 2 cut(s) 762, 1070
Bsh1236I CGCG 2 cut(s) 127, 1208
BshNI GGYRCC 1 cut(s) 817
BsiHKAI GWGCWC 2 cut(s) 193, 1436
BsiSI CCGG 2 cut(s) 318, 949
BslFI GGGAC 3 cut(s) 220, 343, 1250
BslI CCNNNNNNNGG 1 cut(s) 1399
BsmAI GTCTC 2 cut(s) 855, 1007
BsmFI GGGAC 3 cut(s) 220, 343, 1250
Bsp1286I GDGCHC 2 cut(s) 193, 1436
Bsp143I GATC 3 cut(s) 61, 936, 1212
Bsp1720I GCTNAGC 1 cut(s) 117
Bsp19I CCATGG 1 cut(s) 802
BspACI CCGC 8 cut(s) 57, 395, 928, 1024, 1185, 1206, 1208, 1304
BspCNI CTCAG 2 cut(s) 109, 1372
BspFNI CGCG 2 cut(s) 127, 1208
BspLI GGNNCC 3 cut(s) 208, 819, 1258
BspMI ACCTGC 1 cut(s) 1359
BspPI GGATC 2 cut(s) 56, 1220
BspQI GCTCTTC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 817
BsrDI GCAATG 1 cut(s) 1272
BsrI ACTGG 2 cut(s) 1123, 1201
BssECI CCNNGG 4 cut(s) 318, 802, 1098, 1206
BssMI GATC 3 cut(s) 61, 936, 1212
BssSI CACGAG 1 cut(s) 1437
BssT1I CCWWGG 1 cut(s) 802
Bst2BI CACGAG 1 cut(s) 1437
Bst2UI CCWGG 1 cut(s) 1099
Bst4CI ACNGT 3 cut(s) 199, 760, 1177
Bst6I CTCTTC 2 cut(s) 28, 858
BstAPI GCANNNNNTGC 1 cut(s) 888
BstC8I GCNNGC 3 cut(s) 555, 1187, 1296
BstDEI CTNAG 2 cut(s) 117, 1380
BstDSI CCRYGG 2 cut(s) 802, 1206
BstF5I GGATG 7 cut(s) 100, 191, 453, 639, 786, 1099, 1381
BstFNI CGCG 2 cut(s) 127, 1208
BstKTI GATC 3 cut(s) 64, 939, 1215
BstMAI GTCTC 2 cut(s) 855, 1007
BstMBI GATC 3 cut(s) 61, 936, 1212
BstMWI GCNNNNNNNGC 9 cut(s) 133, 485, 550, 622, 888, 981, 1094, 1136, 1414
BstNI CCWGG 1 cut(s) 1099
BstNSI RCATGY 1 cut(s) 767
BstPAI GACNNNNGTC 1 cut(s) 1019
BstSCI CCNGG 2 cut(s) 317, 1097
BstUI CGCG 2 cut(s) 127, 1208
BstV1I GCAGC 5 cut(s) 100, 288, 537, 569, 668
BstV2I GAAGAC 3 cut(s) 572, 1356, 1412
BstX2I RGATCY 1 cut(s) 1212
BstYI RGATCY 1 cut(s) 1212
BtgI CCRYGG 2 cut(s) 802, 1206
BtgZI GCGATG 2 cut(s) 108, 1313
BtsCI GGATG 7 cut(s) 100, 191, 453, 639, 786, 1099, 1381
BtsI GCAGTG 2 cut(s) 923, 1260
BtsIMutI CAGTG 3 cut(s) 839, 923, 1260
BveI ACCTGC 1 cut(s) 1359
Cac8I GCNNGC 3 cut(s) 555, 1187, 1296
CaiI CAGNNNCTG 2 cut(s) 888, 1157
Cfr13I GGNCC 4 cut(s) 207, 645, 951, 1257
Cfr42I CCGCGG 1 cut(s) 1209
CseI GACGC 3 cut(s) 116, 702, 1406
Csp6I GTAC 2 cut(s) 250, 746
CviAII CATG 8 cut(s) 348, 382, 502, 541, 613, 764, 803, 1221
CviQI GTAC 2 cut(s) 250, 746
DdeI CTNAG 2 cut(s) 117, 1380
DpnI GATC 3 cut(s) 63, 938, 1214
DpnII GATC 3 cut(s) 61, 936, 1212
Eam1104I CTCTTC 2 cut(s) 28, 858
EarI CTCTTC 2 cut(s) 28, 858
Ecl136II GAGCTC 1 cut(s) 1434
Eco130I CCWWGG 1 cut(s) 802
Eco24I GRGCYC 1 cut(s) 1436
Eco47I GGWCC 4 cut(s) 207, 645, 951, 1257
Eco53kI GAGCTC 1 cut(s) 1434
EcoICRI GAGCTC 1 cut(s) 1434
EcoRII CCWGG 1 cut(s) 1097
EcoT14I CCWWGG 1 cut(s) 802
EcoT38I GRGCYC 1 cut(s) 1436
ErhI CCWWGG 1 cut(s) 802
FaeI CATG 8 cut(s) 351, 385, 505, 544, 616, 767, 806, 1224
FaqI GGGAC 3 cut(s) 220, 343, 1250
FatI CATG 8 cut(s) 347, 381, 501, 540, 612, 763, 802, 1220
FauI CCCGC 2 cut(s) 1178, 1201
FblI GTMKAC 1 cut(s) 1115
Fnu4HI GCNGC 5 cut(s) 114, 302, 551, 558, 657
FokI GGATG 7 cut(s) 107, 198, 460, 646, 793, 1106, 1388
FriOI GRGCYC 1 cut(s) 1436
Fsp4HI GCNGC 5 cut(s) 114, 302, 551, 558, 657
FspBI CTAG 3 cut(s) 71, 707, 1007
GluI GCNGC 5 cut(s) 114, 302, 551, 558, 657
GsuI CTGGAG 1 cut(s) 1140
HapII CCGG 2 cut(s) 318, 949
HgaI GACGC 3 cut(s) 116, 702, 1406
Hin1II CATG 8 cut(s) 351, 385, 505, 544, 616, 767, 806, 1224
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 724
HinfI GANTC 7 cut(s) 67, 79, 166, 577, 945, 1015, 1363
HpaII CCGG 2 cut(s) 318, 949
HphI GGTGA 1 cut(s) 1447
Hpy166II GTNNAC 8 cut(s) 252, 285, 400, 692, 899, 1116, 1260, 1455
Hpy188I TCNGA 5 cut(s) 66, 84, 165, 921, 1402
Hpy188III TCNNGA 5 cut(s) 406, 565, 584, 1216, 1437
Hpy8I GTNNAC 8 cut(s) 252, 285, 400, 692, 899, 1116, 1260, 1455
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 2 cut(s) 382, 658
HpyCH4III ACNGT 3 cut(s) 199, 760, 1177
HpyCH4V TGCA 9 cut(s) 473, 496, 553, 743, 763, 863, 1051, 1265, 1346
HpyF10VI GCNNNNNNNGC 9 cut(s) 133, 485, 550, 622, 888, 981, 1094, 1136, 1414
HpyF3I CTNAG 2 cut(s) 117, 1380
Hsp92II CATG 8 cut(s) 351, 385, 505, 544, 616, 767, 806, 1224
KspI CCGCGG 1 cut(s) 1209
Kzo9I GATC 3 cut(s) 61, 936, 1212
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 87, 1431
Lsp1109I GCAGC 5 cut(s) 100, 288, 537, 569, 668
LweI GCATC 3 cut(s) 1084, 1366, 1395
MaeI CTAG 3 cut(s) 71, 707, 1007
MaeIII GTNAC 3 cut(s) 137, 865, 1321
MalI GATC 3 cut(s) 63, 938, 1214
MboI GATC 3 cut(s) 61, 936, 1212
MboII GAAGA 7 cut(s) 15, 572, 845, 946, 1361, 1378, 1412
MfeI CAATTG 1 cut(s) 516
MflI RGATCY 1 cut(s) 1212
MhlI GDGCHC 2 cut(s) 193, 1436
MluCI AATT 2 cut(s) 456, 516
MlyI GAGTC 4 cut(s) 88, 586, 954, 1009
MmeI TCCRAC 3 cut(s) 62, 323, 1380
MseI TTAA 3 cut(s) 219, 1032, 1227
MslI CAYNNNNRTG 3 cut(s) 545, 928, 1265
MspA1I CMGCKG 4 cut(s) 59, 560, 659, 1208
MspI CCGG 2 cut(s) 318, 949
MspR9I CCNGG 2 cut(s) 319, 1099
MunI CAATTG 1 cut(s) 516
MvaI CCWGG 1 cut(s) 1099
MvnI CGCG 2 cut(s) 127, 1208
MwoI GCNNNNNNNGC 9 cut(s) 133, 485, 550, 622, 888, 981, 1094, 1136, 1414
NciI CCSGG 1 cut(s) 319
NcoI CCATGG 1 cut(s) 802
NdeII GATC 3 cut(s) 61, 936, 1212
NlaIII CATG 8 cut(s) 351, 385, 505, 544, 616, 767, 806, 1224
NlaIV GGNNCC 3 cut(s) 208, 819, 1258
NmuCI GTSAC 2 cut(s) 137, 865
NspI RCATGY 1 cut(s) 767
OliI CACNNNNGTG 1 cut(s) 928
PaqCI CACCTGC 1 cut(s) 1359
PciSI GCTCTTC 1 cut(s) 28
PfeI GAWTC 3 cut(s) 67, 166, 1363
PkrI GCNGC 5 cut(s) 115, 303, 552, 559, 658
PleI GAGTC 4 cut(s) 87, 585, 953, 1009
PpsI GAGTC 4 cut(s) 87, 585, 953, 1009
PshAI GACNNNNGTC 1 cut(s) 1019
Psp124BI GAGCTC 1 cut(s) 1436
Psp6I CCWGG 1 cut(s) 1097
PspGI CCWGG 1 cut(s) 1097
PspN4I GGNNCC 3 cut(s) 208, 819, 1258
PspPI GGNCC 4 cut(s) 207, 645, 951, 1257
PstNI CAGNNNCTG 2 cut(s) 888, 1157
PsuI RGATCY 1 cut(s) 1212
PvuII CAGCTG 2 cut(s) 560, 659
RsaI GTAC 2 cut(s) 251, 747
RsaNI GTAC 2 cut(s) 250, 746
RseI CAYNNNNRTG 3 cut(s) 545, 928, 1265
SacI GAGCTC 1 cut(s) 1436
SacII CCGCGG 1 cut(s) 1209
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 3 cut(s) 219, 1032, 1227
SatI GCNGC 5 cut(s) 114, 302, 551, 558, 657
Sau3AI GATC 3 cut(s) 61, 936, 1212
Sau96I GGNCC 4 cut(s) 207, 645, 951, 1257
ScaI AGTACT 1 cut(s) 747
SchI GAGTC 4 cut(s) 88, 586, 954, 1009
ScrFI CCNGG 2 cut(s) 319, 1099
SduI GDGCHC 2 cut(s) 193, 1436
SfaNI GCATC 3 cut(s) 1084, 1366, 1395
Sfr303I CCGCGG 1 cut(s) 1209
SgrBI CCGCGG 1 cut(s) 1209
SinI GGWCC 4 cut(s) 207, 645, 951, 1257
SmiMI CAYNNNNRTG 3 cut(s) 545, 928, 1265
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
Sse9I AATT 2 cut(s) 456, 516
SsiI CCGC 8 cut(s) 57, 395, 928, 1024, 1185, 1206, 1208, 1304
SspMI CTAG 3 cut(s) 71, 707, 1007
SstI GAGCTC 1 cut(s) 1436
StyD4I CCNGG 2 cut(s) 317, 1097
StyI CCWWGG 1 cut(s) 802
TaaI ACNGT 3 cut(s) 199, 760, 1177
TaqI TCGA 3 cut(s) 51, 77, 130
TasI AATT 2 cut(s) 456, 516
TatI WGTACW 1 cut(s) 745
TfiI GAWTC 3 cut(s) 67, 166, 1363
Tru1I TTAA 3 cut(s) 219, 1032, 1227
Tru9I TTAA 3 cut(s) 219, 1032, 1227
TscAI CASTG 3 cut(s) 846, 930, 1267
TseFI GTSAC 2 cut(s) 137, 865
TseI GCWGC 5 cut(s) 113, 301, 550, 557, 656
Tsp45I GTSAC 2 cut(s) 137, 865
TspDTI ATGAA 8 cut(s) 17, 158, 401, 490, 601, 860, 904, 992
TspGWI ACGGA 2 cut(s) 302, 353
TspRI CASTG 3 cut(s) 846, 930, 1267
VpaK11BI GGWCC 4 cut(s) 207, 645, 951, 1257
XapI RAATTY 1 cut(s) 456
XceI RCATGY 1 cut(s) 767
XcmI CCANNNNNNNNNTGG 1 cut(s) 129
XmiI GTMKAC 1 cut(s) 1115
XspI CTAG 3 cut(s) 71, 707, 1007
ZrmI AGTACT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.