pycom13g04680

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015722
Physical Location & Seq
Reverse (-)
3123903 .. 3124981
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGGTGGCCTCGTCCTCATCGTTGTCTGAGTACTCCTCGGACTCGTCCTCGTCGTCTTCGTCTGTATCTCGCCGCAGACATCATCCCCACCACCGCAGTTGCCGAGATAAAAACAAAGATGCCTTCAAGATCCGAAAAAAGACCAGTCGCTCCAACACTAAACGACGTCGTAGACACCGCCACCGCCACTCCTCGTCGGATTCTTACTCTTCCTCCTCTCCTTCCGATTACAGGTCAATTTACAAAACCCTTCTTTTATTTTTTGGGCTTATGTATGGTGGCTTTAATTTTGTTATCAGTTTTATCCTCTGTTTGGTTAGGCCTCCTTTCTCTCTCGCTCTGTCTCTCAGGCGACTCGCGTTGTCAGCAAGAGTGGAAGATCCCAACGGCGGATTCGGTTCCCAAGCTCCAACGATTCTGTCTGTGGCAAAGAGAATTCAGGAAGAGTGGAAGATCCTGGAGAAGGATTTGCCAGGCAAGTTCTTCCGTTTTTTTATTTTGAATATCCTTTTAGATGTGCTTACCCCCTTTTCATTGACATCCACGGATACAATAGTTGTTAGAGCTTATGAGGCAATAATGGATTGTGTGAGAGCTGTAATTGTTGGGGCGGAGGGTACTCCTTACCATGATGGTCTCTTCTTCTTTGATGTTTGCTTCCCCAGTGGCTATCCCAATGTACCACCAGTATGTGATAATTTTGAACTCCTAGAATTGATTTGTATCTGTTTACATCTTTGGCCTAACCGGGAACTCTGTTATGCGGAATGTCTACTACCACTCTGGTGGCCTTTGATTTAA

Protein Analysis

267

Amino Acids

30.38

Weight (kDa)

9.27

Isoelectric Point (pI)

72.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 193 - 231 7.4e-07 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 169
AccI GTMKAC 2 cut(s) 172, 772
AccII CGCG 1 cut(s) 359
AciI CCGC 7 cut(s) 73, 94, 178, 184, 390, 611, 764
AclWI GGATC 3 cut(s) 124, 374, 448
AcsI RAATTY 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 3 cut(s) 32, 619, 681
AfiI CCNNNNNNNGG 4 cut(s) 231, 313, 389, 463
AgsI TTSAA 3 cut(s) 127, 502, 704
AjnI CCWGG 2 cut(s) 456, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AleI CACNNNNGTG 1 cut(s) 784
AluBI AGCT 3 cut(s) 407, 566, 596
AluI AGCT 3 cut(s) 407, 566, 596
Alw26I GTCTC 2 cut(s) 348, 641
AlwI GGATC 3 cut(s) 124, 374, 448
AoxI GGCC 4 cut(s) 6, 320, 740, 788
ApoI RAATTY 1 cut(s) 435
AsuC2I CCSGG 1 cut(s) 749
BaeI ACNNNNGTAYC 2 cut(s) 540, 573
BbsI GAAGAC 1 cut(s) 48
BccI CCATC 1 cut(s) 626
BceAI ACGGC 1 cut(s) 403
BciT130I CCWGG 2 cut(s) 458, 474
BciVI GTATCC 1 cut(s) 541
BcnI CCSGG 1 cut(s) 749
BcoDI GTCTC 2 cut(s) 348, 641
BfaI CTAG 1 cut(s) 710
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
BmcAI AGTACT 1 cut(s) 32
Bme1390I CCNGG 3 cut(s) 458, 474, 749
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 3 cut(s) 458, 474, 749
BmrI ACTGGG 1 cut(s) 657
BmsI GCATC 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 657
BpiI GAAGAC 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpmI CTGGAG 1 cut(s) 479
BpuMI CCSGG 1 cut(s) 749
BsaBI GATNNNNATC 1 cut(s) 722
BsaHI GRCGYC 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 2 cut(s) 36, 543
BsaXI ACNNNNNCTCC 4 cut(s) 173, 197, 203, 227
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 313, 389, 463
Bse1I ACTGG 3 cut(s) 144, 663, 686
Bse8I GATNNNNATC 1 cut(s) 722
BseBI CCWGG 2 cut(s) 458, 474
BseDI CCNNGG 2 cut(s) 36, 543
BseGI GGATG 2 cut(s) 82, 539
BseJI GATNNNNATC 1 cut(s) 722
BseLI CCNNNNNNNGG 4 cut(s) 231, 313, 389, 463
BseMII CTCAG 2 cut(s) 18, 361
BseNI ACTGG 3 cut(s) 144, 663, 686
BseRI GAGGAG 3 cut(s) 25, 181, 205
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 4 cut(s) 8, 322, 742, 790
BsiSI CCGG 1 cut(s) 748
BslI CCNNNNNNNGG 4 cut(s) 231, 313, 389, 463
BsmAI GTCTC 2 cut(s) 348, 641
BsnI GGCC 4 cut(s) 8, 322, 742, 790
Bso31I GGTCTC 1 cut(s) 641
Bsp143I GATC 3 cut(s) 129, 379, 453
BspACI CCGC 7 cut(s) 73, 94, 178, 184, 390, 611, 764
BspANI GGCC 4 cut(s) 8, 322, 742, 790
BspCNI CTCAG 2 cut(s) 19, 360
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 3 cut(s) 124, 374, 448
BspTNI GGTCTC 1 cut(s) 641
BsrI ACTGG 3 cut(s) 144, 663, 686
BssECI CCNNGG 2 cut(s) 36, 543
BssMI GATC 3 cut(s) 129, 379, 453
BssNI GRCGYC 1 cut(s) 166
Bst2UI CCWGG 2 cut(s) 458, 474
Bst6I CTCTTC 3 cut(s) 214, 438, 644
BstACI GRCGYC 1 cut(s) 166
BstDEI CTNAG 2 cut(s) 27, 347
BstDSI CCRYGG 1 cut(s) 543
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 2 cut(s) 82, 539
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 3 cut(s) 132, 382, 456
BstMAI GTCTC 2 cut(s) 348, 641
BstMBI GATC 3 cut(s) 129, 379, 453
BstMWI GCNNNNNNNGC 2 cut(s) 365, 572
BstNI CCWGG 2 cut(s) 458, 474
BstSCI CCNGG 3 cut(s) 456, 472, 747
BstUI CGCG 1 cut(s) 359
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 3 cut(s) 129, 379, 453
BstXI CCANNNNNNTGG 1 cut(s) 786
BstYI RGATCY 3 cut(s) 129, 379, 453
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 4 cut(s) 8, 322, 742, 790
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 2 cut(s) 82, 539
BtsIMutI CAGTG 1 cut(s) 670
Csp6I GTAC 3 cut(s) 31, 618, 680
CspCI CAANNNNNGTGG 2 cut(s) 80, 115
CviAII CATG 1 cut(s) 629
CviQI GTAC 3 cut(s) 31, 618, 680
DdeI CTNAG 2 cut(s) 27, 347
DpnI GATC 3 cut(s) 131, 381, 455
DpnII GATC 3 cut(s) 129, 379, 453
Eam1104I CTCTTC 3 cut(s) 214, 438, 644
EarI CTCTTC 3 cut(s) 214, 438, 644
EciI GGCGGA 2 cut(s) 405, 626
Eco147I AGGCCT 1 cut(s) 322
Eco31I GGTCTC 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 456, 472
FaeI CATG 1 cut(s) 632
FaiI YATR 6 cut(s) 272, 276, 570, 630, 691, 762
FatI CATG 1 cut(s) 628
FblI GTMKAC 2 cut(s) 172, 772
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 2 cut(s) 69, 526
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 1 cut(s) 710
GluI GCNGC 1 cut(s) 73
GsuI CTGGAG 1 cut(s) 479
HaeIII GGCC 4 cut(s) 8, 322, 742, 790
HapII CCGG 1 cut(s) 748
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 1 cut(s) 632
HinfI GANTC 5 cut(s) 41, 200, 354, 393, 415
HpaII CCGG 1 cut(s) 748
Hpy166II GTNNAC 3 cut(s) 173, 731, 773
Hpy188I TCNGA 5 cut(s) 28, 40, 134, 199, 226
Hpy188III TCNNGA 2 cut(s) 127, 440
Hpy8I GTNNAC 3 cut(s) 173, 731, 773
Hpy99I CGWCG 4 cut(s) 55, 168, 171, 199
HpyAV CCTTC 4 cut(s) 133, 231, 260, 457
HpyCH4IV ACGT 1 cut(s) 166
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 572
HpyF3I CTNAG 2 cut(s) 27, 347
HpySE526I ACGT 1 cut(s) 166
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 1 cut(s) 632
Kzo9I GATC 3 cut(s) 129, 379, 453
LmnI GCTCC 2 cut(s) 155, 412
LweI GCATC 1 cut(s) 109
MaeI CTAG 1 cut(s) 710
MaeII ACGT 1 cut(s) 166
MalI GATC 3 cut(s) 131, 381, 455
MboI GATC 3 cut(s) 129, 379, 453
MboII GAAGA 8 cut(s) 48, 201, 389, 455, 463, 475, 631, 634
MflI RGATCY 3 cut(s) 129, 379, 453
MluCI AATT 6 cut(s) 237, 286, 435, 600, 697, 713
MlyI GAGTC 2 cut(s) 35, 348
MmeI TCCRAC 3 cut(s) 177, 177, 434
MseI TTAA 2 cut(s) 285, 799
MslI CAYNNNNRTG 2 cut(s) 688, 784
MspI CCGG 1 cut(s) 748
MspR9I CCNGG 3 cut(s) 458, 474, 749
MvaI CCWGG 2 cut(s) 458, 474
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 365, 572
NciI CCSGG 1 cut(s) 749
NdeII GATC 3 cut(s) 129, 379, 453
NlaIII CATG 1 cut(s) 632
NlaIV GGNNCC 1 cut(s) 400
NmeAIII GCCGAG 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 784
PceI AGGCCT 1 cut(s) 322
PcsI WCGNNNNNNNCGW 3 cut(s) 17, 50, 56
PfeI GAWTC 3 cut(s) 200, 393, 415
PfoI TCCNGGA 1 cut(s) 456
PkrI GCNGC 1 cut(s) 74
PleI GAGTC 2 cut(s) 35, 348
PpsI GAGTC 2 cut(s) 35, 348
Psp6I CCWGG 2 cut(s) 456, 472
PspGI CCWGG 2 cut(s) 456, 472
PspN4I GGNNCC 1 cut(s) 400
PsuI RGATCY 3 cut(s) 129, 379, 453
RsaI GTAC 3 cut(s) 32, 619, 681
RsaNI GTAC 3 cut(s) 31, 618, 680
RseI CAYNNNNRTG 2 cut(s) 688, 784
SaqAI TTAA 2 cut(s) 285, 799
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 3 cut(s) 129, 379, 453
ScaI AGTACT 1 cut(s) 32
SchI GAGTC 2 cut(s) 35, 348
ScrFI CCNGG 3 cut(s) 458, 474, 749
SetI ASST 5 cut(s) 169, 236, 409, 568, 598
SfaNI GCATC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 688, 784
Sse9I AATT 6 cut(s) 237, 286, 435, 600, 697, 713
SseBI AGGCCT 1 cut(s) 322
SsiI CCGC 7 cut(s) 73, 94, 178, 184, 390, 611, 764
SspMI CTAG 1 cut(s) 710
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 3 cut(s) 456, 472, 747
TaiI ACGT 1 cut(s) 169
TasI AATT 6 cut(s) 237, 286, 435, 600, 697, 713
TatI WGTACW 1 cut(s) 30
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 3 cut(s) 200, 393, 415
Tru1I TTAA 2 cut(s) 285, 799
Tru9I TTAA 2 cut(s) 285, 799
TscAI CASTG 1 cut(s) 670
TspDTI ATGAA 1 cut(s) 522
TspGWI ACGGA 2 cut(s) 476, 560
TspRI CASTG 1 cut(s) 670
XagI CCTNNNNNAGG 1 cut(s) 461
XapI RAATTY 1 cut(s) 435
XmiI GTMKAC 2 cut(s) 172, 772
XspI CTAG 1 cut(s) 710
ZraI GACGTC 1 cut(s) 167
ZrmI AGTACT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.