pycom13g05430

Ras GTPase-activating protein-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003567
Physical Location & Seq
Forward (+)
3627319 .. 3628986
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGGCAAATCAATCAGAAAACCTACCTACAGCAACGGACGTGGGCAATATTTTCATAAACCAATATTATGATTTCTTTCAAAACGATTCTCAGGTGCTCCATAAATTTTACCAGGAGTCAAGTGTGCTATCTCGTCCTGGACCAGATGGTTCGACGACGACAGTGACAACTATGGAAGGCATTGAGAGCATGCTACTGTCCATGGGCTACCAGAATTATGGTGATTTAGAGATATCAACCACTGATTCTCAGTTTTCATTCAATGGTGGAGTGATTGTTTTAGTGACTGGGTATTTAACTGGGGATGACAACGTGCGAAGAAATTTTACTCAAACTTTCTTTCTAGCCCCGCAACACAACGGATACTTTGTCTTGAACGATATCTTTAAATATGTGGATGTTGTGCCAAGTAATGTCGATGAAAATACACCACAAGCGCCTGTGCCCTCCAACCATGGTATGTTATATTGTATAGGTGCTGGTTTGCTGCCCGCTGCCGTTGCTGATCAGCCCGTGGTCAGTCAAACTACATCTGTGGAGGTTGAAAATACTAATGGAAAGAGAGGGAATGATGTGATGGAAAATGTTCCGGAACCTGTTACTAAATATAGTGTTAGTGCTGATGATCCCATTGAATCAAGACGGAGTGTTGCCCAGAAAGAAACGGAAGCCACAGCCTCTATCACCCAGAATGATGCTCCGAAGAAGTCTTTTGCAGCAGTGGTGTTACATAACTTTCTCGCTGTTGGGAAAAAGTATTCATACTTTGTACCCTTCTTATTTACTGTTGCAGTTAAGTCTCATGCAATTTTCGTCGCCAAGTTGCCCATGGATGCGACTCGTGAAGAACTGGAGAAGATATTCAAGCCTTACGGACCCATCAAGCGCAACGGGATTCAAGTTAGAAGCAATTGGGTATGCATTACGTTTTGTGTGTACTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTF2 RRM_1

Protein Analysis

316

Amino Acids

34.79

Weight (kDa)

4.98

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTF2 PF02136 14 - 131 1.2e-31 Nuclear transport factor 2 (NTF2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 2 cut(s) 350, 492
AclWI GGATC 1 cut(s) 620
AcsI RAATTY 2 cut(s) 104, 322
AfaI GTAC 2 cut(s) 771, 938
AgsI TTSAA 7 cut(s) 80, 262, 376, 545, 635, 865, 899
AjiI CACGTC 1 cut(s) 40
AjnI CCWGG 2 cut(s) 111, 136
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 804
AlwI GGATC 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 589
ApeKI GCWGC 3 cut(s) 487, 494, 716
ApoI RAATTY 2 cut(s) 104, 322
Asp700I GAANNNNTTC 2 cut(s) 585, 860
AspLEI GCGC 2 cut(s) 439, 888
AspS9I GGNCC 2 cut(s) 140, 875
AsuHPI GGTGA 2 cut(s) 233, 676
AvaII GGWCC 2 cut(s) 140, 875
BaeGI GKGCMC 1 cut(s) 447
BarI GAAGNNNNNNTAC 2 cut(s) 310, 342
BauI CACGAG 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 3 cut(s) 474, 481, 728
BccI CCATC 3 cut(s) 140, 571, 887
BceAI ACGGC 1 cut(s) 482
BciT130I CCWGG 2 cut(s) 113, 138
BciVI GTATCC 1 cut(s) 356
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 804
BfaI CTAG 1 cut(s) 344
BfmI CTRYAG 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 440
BfuI GTATCC 1 cut(s) 356
BisI GCNGC 3 cut(s) 488, 495, 717
BlsI GCNGC 3 cut(s) 489, 496, 718
Bme1390I CCNGG 2 cut(s) 113, 138
Bme18I GGWCC 2 cut(s) 140, 875
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 2 cut(s) 140, 875
BmiI GGNNCC 2 cut(s) 594, 877
BmrFI CCNGG 2 cut(s) 113, 138
BmrI ACTGGG 2 cut(s) 297, 309
BmsI GCATC 2 cut(s) 685, 823
BmuI ACTGGG 2 cut(s) 297, 309
BpmI CTGGAG 1 cut(s) 872
BsaJI CCNNGG 4 cut(s) 201, 454, 513, 828
BsaWI WCCGGW 1 cut(s) 589
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bse1I ACTGG 3 cut(s) 292, 304, 855
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 2 cut(s) 113, 138
BseDI CCNNGG 4 cut(s) 201, 454, 513, 828
BseGI GGATG 3 cut(s) 310, 403, 838
BseMII CTCAG 2 cut(s) 104, 263
BseNI ACTGG 3 cut(s) 292, 304, 855
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 3 cut(s) 474, 481, 728
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 590
BsmAI GTCTC 1 cut(s) 804
Bsp1286I GDGCHC 2 cut(s) 99, 447
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 2 cut(s) 505, 625
Bsp19I CCATGG 3 cut(s) 201, 454, 828
BspACI CCGC 2 cut(s) 350, 492
BspCNI CTCAG 2 cut(s) 103, 262
BspEI TCCGGA 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 594, 877
BspPI GGATC 1 cut(s) 620
BsrI ACTGG 3 cut(s) 292, 304, 855
BssECI CCNNGG 4 cut(s) 201, 454, 513, 828
BssMI GATC 2 cut(s) 505, 625
BssSI CACGAG 1 cut(s) 840
BssT1I CCWWGG 3 cut(s) 201, 454, 828
Bst2BI CACGAG 1 cut(s) 840
Bst2UI CCWGG 2 cut(s) 113, 138
Bst4CI ACNGT 3 cut(s) 163, 198, 787
BstC8I GCNNGC 2 cut(s) 191, 492
BstDEI CTNAG 2 cut(s) 90, 249
BstDSI CCRYGG 4 cut(s) 201, 454, 513, 828
BstF5I GGATG 3 cut(s) 310, 403, 838
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 2 cut(s) 439, 888
BstKTI GATC 2 cut(s) 508, 628
BstMAI GTCTC 1 cut(s) 804
BstMBI GATC 2 cut(s) 505, 625
BstMWI GCNNNNNNNGC 2 cut(s) 186, 500
BstNI CCWGG 2 cut(s) 113, 138
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 111, 136
BstSFI CTRYAG 1 cut(s) 27
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 474, 481, 728
BstXI CCANNNNNNTGG 1 cut(s) 218
BsuI GTATCC 1 cut(s) 356
BtgI CCRYGG 4 cut(s) 201, 454, 513, 828
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 3 cut(s) 310, 403, 838
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 3 cut(s) 168, 240, 726
Cac8I GCNNGC 2 cut(s) 191, 492
CfoI GCGC 2 cut(s) 439, 888
Cfr13I GGNCC 2 cut(s) 140, 875
Csp6I GTAC 2 cut(s) 770, 937
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviAII CATG 5 cut(s) 190, 202, 455, 803, 829
CviJI RGCY 6 cut(s) 207, 347, 511, 671, 677, 868
CviKI_1 RGCY 6 cut(s) 207, 347, 511, 671, 677, 868
CviQI GTAC 2 cut(s) 770, 937
DdeI CTNAG 2 cut(s) 90, 249
DpnI GATC 2 cut(s) 507, 627
DpnII GATC 2 cut(s) 505, 625
DraI TTTAAA 1 cut(s) 388
Eco130I CCWWGG 3 cut(s) 201, 454, 828
Eco32I GATATC 2 cut(s) 234, 382
Eco47I GGWCC 2 cut(s) 140, 875
EcoRII CCWGG 2 cut(s) 111, 136
EcoRV GATATC 2 cut(s) 234, 382
EcoT14I CCWWGG 3 cut(s) 201, 454, 828
EcoT22I ATGCAT 1 cut(s) 923
ErhI CCWWGG 3 cut(s) 201, 454, 828
FaeI CATG 5 cut(s) 193, 205, 458, 806, 832
FatI CATG 5 cut(s) 189, 201, 454, 802, 828
FauI CCCGC 2 cut(s) 357, 499
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 488, 495, 717
FokI GGATG 3 cut(s) 317, 410, 845
Fsp4HI GCNGC 3 cut(s) 488, 495, 717
FspBI CTAG 1 cut(s) 344
GlaI GCGC 2 cut(s) 438, 887
GluI GCNGC 3 cut(s) 488, 495, 717
GsuI CTGGAG 1 cut(s) 872
HaeII RGCGCY 1 cut(s) 440
HapII CCGG 1 cut(s) 590
HhaI GCGC 2 cut(s) 439, 888
Hin1II CATG 5 cut(s) 193, 205, 458, 806, 832
Hin6I GCGC 2 cut(s) 437, 886
HinP1I GCGC 2 cut(s) 437, 886
HinfI GANTC 6 cut(s) 86, 116, 245, 635, 838, 895
HpaII CCGG 1 cut(s) 590
HphI GGTGA 2 cut(s) 233, 676
Hpy166II GTNNAC 1 cut(s) 937
Hpy188I TCNGA 2 cut(s) 16, 702
Hpy188III TCNNGA 5 cut(s) 373, 590, 639, 842, 945
Hpy8I GTNNAC 1 cut(s) 937
Hpy99I CGWCG 3 cut(s) 157, 160, 818
HpyAV CCTTC 2 cut(s) 170, 784
HpyCH4III ACNGT 3 cut(s) 163, 198, 787
HpyCH4IV ACGT 3 cut(s) 39, 312, 926
HpyCH4V TGCA 4 cut(s) 716, 791, 806, 921
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 500
HpyF3I CTNAG 2 cut(s) 90, 249
HpySE526I ACGT 3 cut(s) 39, 312, 926
Hsp92II CATG 5 cut(s) 193, 205, 458, 806, 832
HspAI GCGC 2 cut(s) 437, 886
Kpn2I TCCGGA 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 2 cut(s) 505, 625
LmnI GCTCC 2 cut(s) 102, 703
Lsp1109I GCAGC 3 cut(s) 474, 481, 728
LweI GCATC 2 cut(s) 685, 823
MaeI CTAG 1 cut(s) 344
MaeII ACGT 3 cut(s) 39, 312, 926
MaeIII GTNAC 4 cut(s) 163, 283, 598, 726
MalI GATC 2 cut(s) 507, 627
MboI GATC 2 cut(s) 505, 625
MboII GAAGA 5 cut(s) 330, 715, 857, 868, 933
MfeI CAATTG 1 cut(s) 910
MhlI GDGCHC 2 cut(s) 99, 447
MluCI AATT 5 cut(s) 104, 214, 322, 807, 910
MlyI GAGTC 2 cut(s) 125, 832
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 4 cut(s) 457, 532, 557, 688
Mph1103I ATGCAT 1 cut(s) 923
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 585, 860
MseI TTAA 3 cut(s) 296, 387, 795
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 2 cut(s) 113, 138
MunI CAATTG 1 cut(s) 910
MvaI CCWGG 2 cut(s) 113, 138
MwoI GCNNNNNNNGC 2 cut(s) 186, 500
NcoI CCATGG 3 cut(s) 201, 454, 828
NdeII GATC 2 cut(s) 505, 625
NlaIII CATG 5 cut(s) 193, 205, 458, 806, 832
NlaIV GGNNCC 2 cut(s) 594, 877
NmuCI GTSAC 2 cut(s) 163, 283
NsiI ATGCAT 1 cut(s) 923
NspI RCATGY 1 cut(s) 193
PaeI GCATGC 1 cut(s) 193
PdmI GAANNNNTTC 2 cut(s) 585, 860
PfeI GAWTC 4 cut(s) 86, 245, 635, 895
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 3 cut(s) 489, 496, 718
PleI GAGTC 2 cut(s) 124, 832
PpsI GAGTC 2 cut(s) 124, 832
Psp6I CCWGG 2 cut(s) 111, 136
PspGI CCWGG 2 cut(s) 111, 136
PspN4I GGNNCC 2 cut(s) 594, 877
PspPI GGNCC 2 cut(s) 140, 875
RsaI GTAC 2 cut(s) 771, 938
RsaNI GTAC 2 cut(s) 770, 937
SaqAI TTAA 3 cut(s) 296, 387, 795
SatI GCNGC 3 cut(s) 488, 495, 717
Sau3AI GATC 2 cut(s) 505, 625
Sau96I GGNCC 2 cut(s) 140, 875
SchI GAGTC 2 cut(s) 125, 832
ScrFI CCNGG 2 cut(s) 113, 138
SduI GDGCHC 2 cut(s) 99, 447
SetI ASST 9 cut(s) 24, 28, 42, 96, 315, 478, 543, 598, 929
SfaNI GCATC 2 cut(s) 685, 823
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 2 cut(s) 140, 875
SphI GCATGC 1 cut(s) 193
Sse9I AATT 5 cut(s) 104, 214, 322, 807, 910
SsiI CCGC 2 cut(s) 350, 492
SspI AATATT 2 cut(s) 49, 65
SspMI CTAG 1 cut(s) 344
StyD4I CCNGG 2 cut(s) 111, 136
StyI CCWWGG 3 cut(s) 201, 454, 828
TaaI ACNGT 3 cut(s) 163, 198, 787
TaiI ACGT 3 cut(s) 42, 315, 929
TaqI TCGA 2 cut(s) 152, 417
TasI AATT 5 cut(s) 104, 214, 322, 807, 910
TatI WGTACW 1 cut(s) 936
TfiI GAWTC 4 cut(s) 86, 245, 635, 895
Tru1I TTAA 3 cut(s) 296, 387, 795
Tru9I TTAA 3 cut(s) 296, 387, 795
TscAI CASTG 3 cut(s) 168, 247, 726
TseFI GTSAC 2 cut(s) 163, 283
TseI GCWGC 3 cut(s) 487, 494, 716
Tsp45I GTSAC 2 cut(s) 163, 283
TspDTI ATGAA 4 cut(s) 43, 246, 435, 750
TspGWI ACGGA 5 cut(s) 50, 375, 658, 680, 888
TspRI CASTG 3 cut(s) 168, 247, 726
VpaK11BI GGWCC 2 cut(s) 140, 875
XapI RAATTY 2 cut(s) 104, 322
XceI RCATGY 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmnI GAANNNNTTC 2 cut(s) 585, 860
XspI CTAG 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.