Rorug04G0307900

Ras GTPase-activating protein-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003567
Physical Location & Seq
Reverse (-)
48344888 .. 48347337
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0307900.1

Sequence Viewer

Length: 1173 bp
ATGGAGCTTCGGAGTTCGTGGATGGTAGCGTTAACGTTGACGGCGGTGATGGTTACGGCCGTAACGGCAGACCGGAGTAATAACAAGTCGGGAGGTTTATACTGGTCAACGGCGGAAGAGGAGGCTCAGGCTCAGCTTCAGCCTAGGGTTGACGATTCCGCCGTGGACGCCGACTTCGACGCCGACGGAGGCTTCTCCTCGCTCGATTCAATGTTGCAGTGGGCTTTGGGTCACTCCGATCCCGATCAATTGAAAGAAGCGGCGAAAGAGGTGCAGGGATTGAACCCTTCCGACCTGAACAAGCGGCGGCTCGAGATAAAGCAGCTCATGGAGGAGCTCAAAACGCCGTCGGATGCTCAGCTGATGAAGGTGGCGATACTGGACCTGAAGAACTCGTCGTTGTCGCTGGAAGATCGGCACCGGGCTTTGCAGGAGCTGCTGGAGCTTGTTGAGCCGATTGACAATGCAAATGATTTGAACAAGCTTGGAGGACTTGCTGTGGTGATAGGAGAGCTTGATCATTCTGATTCAGAGACGAGGAAAATGGCTGCATGGATTATTGGGAAGGCTAGTCAGAACAATCCACTTGTTCAGAAGCAGGTTTTGGAACTTGGGGCACTGTCAAAGCTAATGAAGATGGTAAAATCGAGCTTTGCGGAAGAAGCCATTAAGGCAATTTATGCTGTTTCAGCCTTGCTGAGGAATAATGTAGCTGGTCAGGAAATGTTCTATGAGGAATCTGGGCAAAAACTGCTTCAGGACATTGTGAGTGATTCGAGCATTGGTAACAGACTGCGCATAAGAGCTGTGGTTCTTCTGGGAGATCTTGCAGAGTTTCAATTAGGAAATACGGATAAGGACGAGCAGCCCTTTTTCAGTAATCGCATTTTCTTGAAGTCTGTTGTTGATCTAACATCAGCTGCTGATCTTCACCTACAGGAAAAGGCCCTCATTGCGATTAAGAGTCTTCTGCGACTCAAGACAACTGAAGCTCTGGTGTTTGAGGACTTTTGTGGGTTGGATGCTGCATTGGAGAGAATGAGGCAGCAGCTGCAGGACTTAATGGTAGAGGAATACCACAGAGATTATGCAATGGATGTAGAAAGCCTCCGCAGTGAAGTGCAGCTGATTTTCCAAAGAAAGCTGGAAAAGGTAACACGGATGACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTF2 RRM_1

Protein Analysis

390

Amino Acids

43.51

Weight (kDa)

4.85

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fes1 PF08609 68 - 159 2.5e-17 Nucleotide exchange factor Fes1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 417
AciI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 656, 1111
AclI AACGTT 1 cut(s) 35
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 4 cut(s) 122, 407, 740, 1008
AcyI GRCGYC 2 cut(s) 168, 180
AfiI CCNNNNNNNGG 1 cut(s) 699
AgsI TTSAA 6 cut(s) 210, 253, 283, 478, 839, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 3 cut(s) 436, 923, 1051
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 57, 945
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspA2I CCTAGG 1 cut(s) 143
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 2 cut(s) 382, 946
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 58, 514, 923
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 382
AvrII CCTAGG 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 959
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 698
BccI CCATC 3 cut(s) 16, 43, 631
BceAI ACGGC 7 cut(s) 44, 57, 72, 81, 126, 146, 331
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 2 cut(s) 144, 570
BfmI CTRYAG 2 cut(s) 935, 1052
BfuAI ACCTGC 1 cut(s) 589
BglI GCCNNNNNGGC 2 cut(s) 65, 671
BglII AGATCT 1 cut(s) 823
BlnI CCTAGG 1 cut(s) 143
BlpI GCTNAGC 2 cut(s) 132, 357
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 382, 946
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 2 cut(s) 343, 1012
BpiI GAAGAC 1 cut(s) 959
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 126, 698
Bpu1102I GCTNAGC 2 cut(s) 132, 357
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 243
BsaHI GRCGYC 2 cut(s) 168, 180
BsaJI CCNNGG 2 cut(s) 143, 162
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse1I ACTGG 2 cut(s) 107, 384
Bse3DI GCAATG 2 cut(s) 951, 1098
Bse8I GATNNNNATC 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 143, 162
BseGI GGATG 5 cut(s) 27, 358, 1027, 1102, 1167
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 699
BseMI GCAATG 2 cut(s) 951, 1098
BseMII CTCAG 4 cut(s) 140, 146, 371, 689
BseNI ACTGG 2 cut(s) 107, 384
BseRI GAGGAG 3 cut(s) 134, 187, 347
BseSI GKGCMC 1 cut(s) 619
BseX3I CGGCCG 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 293, 1142
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 947
BshNI GGYRCC 1 cut(s) 417
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 2 cut(s) 73, 421
BslI CCNNNNNNNGG 1 cut(s) 699
BsmAI GTCTC 1 cut(s) 527
BsmBI CGTCTC 1 cut(s) 527
BsnI GGCC 2 cut(s) 59, 947
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 339, 619
Bsp143I GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
Bsp1720I GCTNAGC 2 cut(s) 132, 357
BspACI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 656, 1111
BspANI GGCC 2 cut(s) 59, 947
BspCNI CTCAG 4 cut(s) 139, 145, 370, 690
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 1 cut(s) 1056
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 417
BsrDI GCAATG 2 cut(s) 951, 1098
BsrI ACTGG 2 cut(s) 107, 384
BssECI CCNNGG 2 cut(s) 143, 162
BssMI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
BssNI GRCGYC 2 cut(s) 168, 180
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 2 cut(s) 168, 180
BstAPI GCANNNNNTGC 4 cut(s) 436, 680, 751, 1051
BstDEI CTNAG 4 cut(s) 126, 132, 357, 698
BstDSI CCRYGG 1 cut(s) 162
BstENI CCTNNNNNAGG 1 cut(s) 697
BstF5I GGATG 5 cut(s) 27, 358, 1027, 1102, 1167
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 7 cut(s) 241, 247, 415, 520, 826, 910, 928
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
BstMCI CGRYCG 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 2 cut(s) 935, 1052
BstSLI GKGCMC 1 cut(s) 619
BstV2I GAAGAC 1 cut(s) 959
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 947
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 5 cut(s) 27, 358, 1027, 1102, 1167
BtsI GCAGTG 2 cut(s) 224, 1120
BtsIMutI CAGTG 3 cut(s) 224, 617, 1120
BveI ACCTGC 1 cut(s) 589
CaiI CAGNNNCTG 3 cut(s) 436, 923, 1051
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 2 cut(s) 382, 946
CseI GACGC 2 cut(s) 176, 188
CviAII CATG 3 cut(s) 328, 552, 1170
DdeI CTNAG 4 cut(s) 126, 132, 357, 698
DpnI GATC 7 cut(s) 240, 246, 414, 519, 825, 909, 927
DpnII GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 2 cut(s) 128, 148
Ecl136II GAGCTC 1 cut(s) 337
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 382
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 4 cut(s) 122, 407, 740, 1008
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 946
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 3 cut(s) 331, 555, 1173
FaiI YATR 8 cut(s) 100, 329, 553, 681, 732, 800, 1089, 1171
FatI CATG 3 cut(s) 327, 551, 1169
FbaI TGATCA 1 cut(s) 517
FokI GGATG 4 cut(s) 34, 365, 1034, 1109
FriOI GRGCYC 1 cut(s) 339
FspBI CTAG 2 cut(s) 144, 570
FspI TGCGCA 1 cut(s) 797
GlaI GCGC 1 cut(s) 797
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 2 cut(s) 59, 947
HapII CCGG 2 cut(s) 73, 421
HgaI GACGC 2 cut(s) 176, 188
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 2 cut(s) 168, 180
Hin1II CATG 3 cut(s) 331, 555, 1173
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 4 cut(s) 33, 39, 108, 151
HindII GTYRAC 4 cut(s) 33, 39, 108, 151
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 7 cut(s) 155, 206, 527, 737, 773, 964, 975
HpaI GTTAAC 1 cut(s) 33
HpaII CCGG 2 cut(s) 73, 421
HphI GGTGA 3 cut(s) 58, 514, 923
Hpy166II GTNNAC 5 cut(s) 33, 39, 108, 151, 166
Hpy188I TCNGA 8 cut(s) 12, 238, 292, 352, 526, 532, 576, 594
Hpy188III TCNNGA 7 cut(s) 90, 242, 313, 719, 758, 892, 979
Hpy8I GTNNAC 5 cut(s) 33, 39, 108, 151, 166
Hpy99I CGWCG 4 cut(s) 182, 188, 352, 400
HpyAV CCTTC 3 cut(s) 297, 361, 559
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4IV ACGT 1 cut(s) 35
HpyF3I CTNAG 4 cut(s) 126, 132, 357, 698
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 2 cut(s) 168, 180
Hsp92II CATG 3 cut(s) 331, 555, 1173
HspAI GCGC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 517
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
LmnI GCTCC 4 cut(s) 4, 334, 433, 442
LweI GCATC 2 cut(s) 343, 1012
MaeI CTAG 2 cut(s) 144, 570
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 5 cut(s) 52, 61, 230, 785, 1153
MalI GATC 7 cut(s) 240, 246, 414, 519, 825, 909, 927
MboI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
MboII GAAGA 8 cut(s) 128, 400, 422, 646, 671, 806, 920, 959
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 2 cut(s) 339, 619
MluCI AATT 3 cut(s) 248, 675, 839
MlyI GAGTC 2 cut(s) 969, 973
MmeI TCCRAC 3 cut(s) 315, 330, 999
MseI TTAA 4 cut(s) 32, 669, 960, 1061
MspA1I CMGCKG 4 cut(s) 361, 920, 1051, 1126
MspI CCGG 2 cut(s) 73, 421
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 248
NciI CCSGG 1 cut(s) 422
NdeII GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
NlaIII CATG 3 cut(s) 331, 555, 1173
NlaIV GGNNCC 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 230
NsbI TGCGCA 1 cut(s) 797
PaeR7I CTCGAG 1 cut(s) 311
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 174
PfeI GAWTC 5 cut(s) 155, 206, 527, 737, 773
PleI GAGTC 2 cut(s) 969, 972
PpsI GAGTC 2 cut(s) 969, 972
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 2 cut(s) 382, 946
PstI CTGCAG 1 cut(s) 1056
PstNI CAGNNNCTG 3 cut(s) 436, 923, 1051
PsuI RGATCY 1 cut(s) 823
PvuII CAGCTG 4 cut(s) 361, 920, 1051, 1126
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 4 cut(s) 32, 669, 960, 1061
Sau3AI GATC 7 cut(s) 238, 244, 412, 517, 823, 907, 925
Sau96I GGNCC 2 cut(s) 382, 946
SchI GAGTC 2 cut(s) 969, 973
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 2 cut(s) 339, 619
SfaNI GCATC 2 cut(s) 343, 1012
SfcI CTRYAG 2 cut(s) 935, 1052
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 311, 977
SmoI CTYRAG 2 cut(s) 311, 977
Sse9I AATT 3 cut(s) 248, 675, 839
SsiI CCGC 8 cut(s) 44, 113, 159, 260, 304, 307, 656, 1111
SspMI CTAG 2 cut(s) 144, 570
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 621
TaiI ACGT 1 cut(s) 38
TaqI TCGA 5 cut(s) 177, 204, 312, 647, 776
TasI AATT 3 cut(s) 248, 675, 839
TauI GCSGC 3 cut(s) 263, 307, 310
TfiI GAWTC 5 cut(s) 155, 206, 527, 737, 773
Tru1I TTAA 4 cut(s) 32, 669, 960, 1061
Tru9I TTAA 4 cut(s) 32, 669, 960, 1061
TscAI CASTG 3 cut(s) 224, 624, 1120
TseFI GTSAC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 380, 647
TspGWI ACGGA 2 cut(s) 201, 866
TspRI CASTG 3 cut(s) 224, 624, 1120
VpaK11BI GGWCC 1 cut(s) 382
XagI CCTNNNNNAGG 1 cut(s) 697
XhoI CTCGAG 1 cut(s) 311
XmaJI CCTAGG 1 cut(s) 143
XspI CTAG 2 cut(s) 144, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.