Rorug04G0308100

Ras GTPase-activating protein-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003567
Physical Location & Seq
Forward (+)
48353576 .. 48355394
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0308100.1

Sequence Viewer

Length: 1092 bp
ATGAATATGATAGCCGAATTACTATTGAGGGTAGTCTGTTTTGTAGCAGCAGCACTGATACCACTTGTGAGCTTAAGTCTTATCAAGGGATGTTCTTGGACATGGTCATGGACCTCATGCTCATGGTCATGGTCAAGTTCATGGCGCCTACCATTTGTGACTCTCTCCCTTAATGATTCTGCTCTAGGAAGACTGCTCTCTTGCTTCAATAGCCACAGACACAGAGTACTATGCGAGTTAGTCGATGACTCTTATCCTCCTGCTGATCAGGAACAAGAACCTTGTGAAGTGACTGAAGAAGATTTGAAAGTATTGGTCTCTCATATTGAGGAGACTGATGGCGGTCCACCTTGGAATCTCATGATGGAGCAGTCTATTCCTTCCTTGACATACAAAGCTTGGTATCGCGATCCACCGTTAGGCCCAACACAGTACCGTACTAAAACAGTTTTCGAAAATGTATCACCAGAGGTATTGAGGGATTTCTTCTGGGATGATGAATTCAGACCCAGGTGGGATAAGACGCTCATTCACACCAAGGTTCTAAGCATTTGCCTCCAAACTGGTACCCTGATTGTTCATTGGATTAGAAAGCTACCACTATTCTGCAGCAATCGAGAATATATCATAATCCGTCGCATTTTTGAATCTGCATCAAGGTATTATTGCGTGACAAAGGCAACACCATATCCATCCATACCAAGGCTAAGAAAACCAAAACGAGTAGATGTCTATCATTCAAGTTGGCATATAAGACCAGTGAAACCCAGGAATGCAGATCAGCAAATGGGATCAGAAGTGCATCTATTTCACTACGAAGACATATGTCTCCCAAGAGAGGTTGTCAAAATGGGCGTCAGGGCAGGAATGTGGAACATGGTAAAGAAACTACATTTAGGAGTTCAGGTTTACAGTATGGAAAAAGCAAATGATGAATCTCCTTCATACCATGCACTTATGGCACGCATAACTACAAAGCTTCCGGCTTCACCATCCACAATCATGTATCACATCCCAGAAGTTGAAGCAGATGAGAATGCATGCCAGAGCCAAAACCCACAAGAACAAGTAACAGAGTTCATAGTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTF2 RRM_1

Protein Analysis

363

Amino Acids

42.12

Weight (kDa)

6.64

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 118 - 294 2.1e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 566
AccB1I GGYRCC 2 cut(s) 144, 566
AccII CGCG 1 cut(s) 408
AciI CCGC 1 cut(s) 342
AclWI GGATC 2 cut(s) 404, 799
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 1 cut(s) 315
AcyI GRCGYC 2 cut(s) 145, 855
AfaI GTAC 4 cut(s) 228, 434, 439, 568
AfiI CCNNNNNNNGG 3 cut(s) 419, 702, 838
AflII CTTAAG 1 cut(s) 73
AgsI TTSAA 5 cut(s) 208, 307, 647, 741, 1025
AjnI CCWGG 2 cut(s) 509, 767
AluBI AGCT 4 cut(s) 72, 398, 595, 979
AluI AGCT 4 cut(s) 72, 398, 595, 979
Alw26I GTCTC 3 cut(s) 322, 326, 833
AlwI GGATC 2 cut(s) 404, 799
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 3 cut(s) 47, 50, 609
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 840, 872
Asp718I GGTACC 1 cut(s) 566
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 111, 344, 422
AsuHPI GGTGA 2 cut(s) 456, 981
AsuII TTCGAA 1 cut(s) 453
AvaII GGWCC 2 cut(s) 111, 344
BanI GGYRCC 2 cut(s) 144, 566
BbsI GAAGAC 2 cut(s) 196, 825
BbvI GCAGC 3 cut(s) 59, 62, 621
BccI CCATC 4 cut(s) 332, 358, 700, 1000
BciT130I CCWGG 2 cut(s) 511, 769
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 3 cut(s) 322, 326, 833
BfaI CTAG 1 cut(s) 185
BfmI CTRYAG 1 cut(s) 607
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 73
BisI GCNGC 3 cut(s) 48, 51, 610
BlsI GCNGC 3 cut(s) 49, 52, 611
BmcAI AGTACT 1 cut(s) 228
Bme1390I CCNGG 2 cut(s) 511, 769
Bme18I GGWCC 2 cut(s) 111, 344
BmgT120I GGNCC 3 cut(s) 111, 344, 422
BmiI GGNNCC 2 cut(s) 146, 568
BmrFI CCNGG 2 cut(s) 511, 769
BmsI GCATC 2 cut(s) 662, 811
BoxI GACNNNNGTC 1 cut(s) 825
BpiI GAAGAC 2 cut(s) 196, 825
Bpu14I TTCGAA 1 cut(s) 453
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 2 cut(s) 145, 855
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 5 cut(s) 350, 509, 537, 701, 767
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 702, 838
Bse1I ACTGG 2 cut(s) 568, 758
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 511, 769
BseDI CCNNGG 5 cut(s) 350, 509, 537, 701, 767
BseGI GGATG 5 cut(s) 95, 499, 692, 992, 1011
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 3 cut(s) 419, 702, 838
BseNI ACTGG 2 cut(s) 568, 758
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 59, 62, 621
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 2 cut(s) 144, 566
BsiSI CCGG 1 cut(s) 983
BslI CCNNNNNNNGG 3 cut(s) 419, 702, 838
BsmAI GTCTC 3 cut(s) 322, 326, 833
BsmI GAATGC 2 cut(s) 778, 1042
BsnI GGCC 1 cut(s) 423
Bso31I GGTCTC 1 cut(s) 322
Bsp119I TTCGAA 1 cut(s) 453
Bsp143I GATC 4 cut(s) 265, 409, 778, 791
Bsp68I TCGCGA 1 cut(s) 408
BspACI CCGC 1 cut(s) 342
BspANI GGCC 1 cut(s) 423
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 146, 568
BspMAI CTGCAG 1 cut(s) 611
BspPI GGATC 2 cut(s) 404, 799
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 2 cut(s) 144, 566
BspTI CTTAAG 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 322
BsrI ACTGG 2 cut(s) 568, 758
BssECI CCNNGG 5 cut(s) 350, 509, 537, 701, 767
BssMI GATC 4 cut(s) 265, 409, 778, 791
BssNI GRCGYC 2 cut(s) 145, 855
BssT1I CCWWGG 3 cut(s) 350, 537, 701
Bst2UI CCWGG 2 cut(s) 511, 769
Bst4CI ACNGT 5 cut(s) 417, 432, 437, 448, 914
BstACI GRCGYC 2 cut(s) 145, 855
BstAFI CTTAAG 1 cut(s) 73
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 964, 1042
BstDEI CTNAG 2 cut(s) 545, 707
BstF5I GGATG 5 cut(s) 95, 499, 692, 992, 1011
BstFNI CGCG 1 cut(s) 408
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 4 cut(s) 268, 412, 781, 794
BstMAI GTCTC 3 cut(s) 322, 326, 833
BstMBI GATC 4 cut(s) 265, 409, 778, 791
BstMWI GCNNNNNNNGC 2 cut(s) 210, 959
BstNI CCWGG 2 cut(s) 511, 769
BstNSI RCATGY 1 cut(s) 1044
BstPAI GACNNNNGTC 1 cut(s) 825
BstSCI CCNGG 2 cut(s) 509, 767
BstSFI CTRYAG 1 cut(s) 607
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 3 cut(s) 59, 62, 621
BstV2I GAAGAC 2 cut(s) 196, 825
BsuRI GGCC 1 cut(s) 423
BtsCI GGATG 5 cut(s) 95, 499, 692, 992, 1011
BtsIMutI CAGTG 2 cut(s) 53, 765
BtuMI TCGCGA 1 cut(s) 408
Cac8I GCNNGC 2 cut(s) 964, 1042
CciI TCATGA 1 cut(s) 360
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 3 cut(s) 111, 344, 422
CseI GACGC 2 cut(s) 532, 844
Csp6I GTAC 4 cut(s) 227, 433, 438, 567
CviQI GTAC 4 cut(s) 227, 433, 438, 567
DdeI CTNAG 2 cut(s) 545, 707
DinI GGCGCC 1 cut(s) 146
DpnI GATC 4 cut(s) 267, 411, 780, 793
DpnII GATC 4 cut(s) 265, 409, 778, 791
Eco130I CCWWGG 3 cut(s) 350, 537, 701
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 111, 344
Eco57I CTGAAG 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 2 cut(s) 509, 767
EcoT14I CCWWGG 3 cut(s) 350, 537, 701
EcoT22I ATGCAT 1 cut(s) 1042
EgeI GGCGCC 1 cut(s) 146
EheI GGCGCC 1 cut(s) 146
ErhI CCWWGG 3 cut(s) 350, 537, 701
FauNDI CATATG 1 cut(s) 824
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 3 cut(s) 48, 51, 610
FokI GGATG 5 cut(s) 102, 506, 679, 979, 998
Fsp4HI GCNGC 3 cut(s) 48, 51, 610
FspBI CTAG 1 cut(s) 185
GlaI GCGC 1 cut(s) 146
GluI GCNGC 3 cut(s) 48, 51, 610
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 1 cut(s) 423
HapII CCGG 1 cut(s) 983
HgaI GACGC 2 cut(s) 532, 844
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 2 cut(s) 145, 855
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 2 cut(s) 396, 977
HinfI GANTC 6 cut(s) 160, 176, 248, 355, 647, 935
HpaII CCGG 1 cut(s) 983
HphI GGTGA 2 cut(s) 456, 981
Hpy166II GTNNAC 2 cut(s) 347, 910
Hpy188I TCNGA 2 cut(s) 506, 796
Hpy188III TCNNGA 4 cut(s) 269, 361, 407, 617
Hpy8I GTNNAC 2 cut(s) 347, 910
Hpy99I CGWCG 1 cut(s) 639
HpyAV CCTTC 2 cut(s) 390, 951
HpyCH4III ACNGT 5 cut(s) 417, 432, 437, 448, 914
HpyCH4V TGCA 6 cut(s) 609, 653, 776, 802, 953, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 959
HpyF3I CTNAG 2 cut(s) 545, 707
Hsp92I GRCGYC 2 cut(s) 145, 855
HspAI GCGC 1 cut(s) 145
KasI GGCGCC 1 cut(s) 144
KpnI GGTACC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 265, 409, 778, 791
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 3 cut(s) 59, 62, 621
LweI GCATC 2 cut(s) 662, 811
MaeI CTAG 1 cut(s) 185
MaeIII GTNAC 4 cut(s) 157, 289, 670, 1069
MalI GATC 4 cut(s) 267, 411, 780, 793
MboI GATC 4 cut(s) 265, 409, 778, 791
MboII GAAGA 5 cut(s) 201, 308, 311, 478, 830
MluCI AATT 2 cut(s) 17, 500
Mly113I GGCGCC 1 cut(s) 145
MlyI GAGTC 2 cut(s) 154, 242
MnlI CCTC 8 cut(s) 21, 124, 267, 322, 463, 471, 566, 832
Mph1103I ATGCAT 1 cut(s) 1042
MseI TTAA 2 cut(s) 74, 171
MslI CAYNNNNRTG 4 cut(s) 106, 121, 127, 1001
MspCI CTTAAG 1 cut(s) 73
MspI CCGG 1 cut(s) 983
MspR9I CCNGG 2 cut(s) 511, 769
Mva1269I GAATGC 2 cut(s) 778, 1042
MvaI CCWGG 2 cut(s) 511, 769
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 2 cut(s) 210, 959
NarI GGCGCC 1 cut(s) 145
NdeI CATATG 1 cut(s) 824
NdeII GATC 4 cut(s) 265, 409, 778, 791
NlaIV GGNNCC 2 cut(s) 146, 568
NmuCI GTSAC 3 cut(s) 157, 289, 670
NruI TCGCGA 1 cut(s) 408
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 1 cut(s) 1044
NspV TTCGAA 1 cut(s) 453
PaeI GCATGC 1 cut(s) 1044
PagI TCATGA 1 cut(s) 360
PctI GAATGC 2 cut(s) 778, 1042
PfeI GAWTC 4 cut(s) 176, 355, 647, 935
PflFI GACNNNGTC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 49, 52, 611
PleI GAGTC 2 cut(s) 154, 242
PluTI GGCGCC 1 cut(s) 148
PpsI GAGTC 2 cut(s) 154, 242
PshAI GACNNNNGTC 1 cut(s) 825
Psp6I CCWGG 2 cut(s) 509, 767
PspGI CCWGG 2 cut(s) 509, 767
PspN4I GGNNCC 2 cut(s) 146, 568
PspPI GGNCC 3 cut(s) 111, 344, 422
PstI CTGCAG 1 cut(s) 611
PsyI GACNNNGTC 1 cut(s) 103
RruI TCGCGA 1 cut(s) 408
RsaI GTAC 4 cut(s) 228, 434, 439, 568
RsaNI GTAC 4 cut(s) 227, 433, 438, 567
RseI CAYNNNNRTG 4 cut(s) 106, 121, 127, 1001
SaqAI TTAA 2 cut(s) 74, 171
SatI GCNGC 3 cut(s) 48, 51, 610
Sau3AI GATC 4 cut(s) 265, 409, 778, 791
Sau96I GGNCC 3 cut(s) 111, 344, 422
ScaI AGTACT 1 cut(s) 228
SchI GAGTC 2 cut(s) 154, 242
ScrFI CCNGG 2 cut(s) 511, 769
SfaNI GCATC 2 cut(s) 662, 811
SfcI CTRYAG 1 cut(s) 607
SfoI GGCGCC 1 cut(s) 146
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 2 cut(s) 111, 344
SmiMI CAYNNNNRTG 4 cut(s) 106, 121, 127, 1001
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
SphI GCATGC 1 cut(s) 1044
Sse9I AATT 2 cut(s) 17, 500
SsiI CCGC 1 cut(s) 342
SspDI GGCGCC 1 cut(s) 144
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 509, 767
StyI CCWWGG 3 cut(s) 350, 537, 701
TaaI ACNGT 5 cut(s) 417, 432, 437, 448, 914
TaqI TCGA 3 cut(s) 243, 453, 616
TasI AATT 2 cut(s) 17, 500
TatI WGTACW 1 cut(s) 226
TfiI GAWTC 4 cut(s) 176, 355, 647, 935
Tru1I TTAA 2 cut(s) 74, 171
Tru9I TTAA 2 cut(s) 74, 171
TscAI CASTG 2 cut(s) 60, 765
TseFI GTSAC 3 cut(s) 157, 289, 670
TseI GCWGC 3 cut(s) 47, 50, 609
Tsp45I GTSAC 3 cut(s) 157, 289, 670
TspDTI ATGAA 7 cut(s) 17, 129, 513, 569, 933, 948, 1069
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 60, 765
Tth111I GACNNNGTC 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 111, 344
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 1044
XspI CTAG 1 cut(s) 185
ZrmI AGTACT 1 cut(s) 228
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.