pycom13g05640

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009683
Physical Location & Seq
Forward (+)
3714585 .. 3715479
895 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGGCATCCTCAGCTTCCTCGCAGTCCGCTGACAAGTACGAGGGGTTTGATCTGTTGAAGTGTTCGACCGTCCAAGAGGGGAAGAAGGAAGCGGATGTCAGGTTACCGTTAGGGTATCGATTTAATCCCACCGAAGATGAGATCGTTGTGTACTTTCTGTTTAACAAGATTATGGACCGCGCAATGCCTACCTACGGTCTTATAAAAGAGGTTGATGTGTACGAGTATGATCCCCATCAGCTGCCGAATGGTGACTTCAAACACAATGCTGGTTGCAATGCGGCCTATTACTTCGCCAACAGAGAACCCTTTGACGCTAGTGAAGGGAAGATAATTAAGACGGCTGCAAACGGTGGTTACTGGAAGGTGATCGATGACGAATATGAGGTTTTGTTCAAGGATAGTGAAGTAATCGTAGGGTTTGAAGCCGTCATGAACTTCTACGAGGGGAAGGCACCAAACGGGACCAAAACTCCCTTTGTCATGAACGAATACAGGCTCAATCCTCGTGTAGTACCTGCTCATGTGCTGAATGACAGCATTAGGAATAAGATTGAGAGGTACGTGGTATGCCGAATTATAAACGAGGAGGTTTCGAATCAGCCCGTTATCGACTATAGAAAGGAATCTCTCGAGCTTCTTTTGAAATATGCGTCTGCCGGCACTGTTAAAGACGTAGAACCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

230

Amino Acids

26.07

Weight (kDa)

5.08

Isoelectric Point (pI)

26.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 36 - 167 2.4e-26 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 203, 581
Acc36I ACCTGC 1 cut(s) 526
AccB1I GGYRCC 1 cut(s) 454
AccII CGCG 1 cut(s) 180
AciI CCGC 4 cut(s) 27, 92, 178, 281
AclWI GGATC 1 cut(s) 224
AfaI GTAC 5 cut(s) 38, 152, 221, 516, 563
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 5 cut(s) 58, 259, 397, 425, 646
AluBI AGCT 3 cut(s) 14, 241, 637
AluI AGCT 3 cut(s) 14, 241, 637
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 2 cut(s) 241, 344
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 2 cut(s) 175, 465
AsuHPI GGTGA 2 cut(s) 263, 379
AsuII TTCGAA 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 2 cut(s) 175, 465
BanI GGYRCC 1 cut(s) 454
BauI CACGAG 1 cut(s) 507
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 2 cut(s) 228, 331
BccI CCATC 1 cut(s) 243
BceAI ACGGC 2 cut(s) 357, 413
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 616
BfuAI ACCTGC 1 cut(s) 526
BisI GCNGC 3 cut(s) 242, 282, 345
BlsI GCNGC 3 cut(s) 243, 283, 346
Bme18I GGWCC 2 cut(s) 175, 465
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 175, 465
BmiI GGNNCC 2 cut(s) 456, 466
BmsI GCATC 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 596
Bsa29I ATCGAT 2 cut(s) 118, 372
BsaAI YACGTR 1 cut(s) 565
BsaBI GATNNNNATC 1 cut(s) 234
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 365
Bse3DI GCAATG 2 cut(s) 189, 283
Bse8I GATNNNNATC 1 cut(s) 234
BseCI ATCGAT 2 cut(s) 118, 372
BseGI GGATG 2 cut(s) 5, 100
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 2 cut(s) 189, 283
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 2 cut(s) 228, 331
Bsh1236I CGCG 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 284
BshNI GGYRCC 1 cut(s) 454
BshVI ATCGAT 2 cut(s) 118, 372
BsiEI CGRYCG 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 478
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 632
Bsp119I TTCGAA 1 cut(s) 596
Bsp143I GATC 4 cut(s) 49, 141, 229, 369
BspACI CCGC 4 cut(s) 27, 92, 178, 281
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 23
BspDI ATCGAT 2 cut(s) 118, 372
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 2 cut(s) 432, 483
BspLI GGNNCC 2 cut(s) 456, 466
BspMI ACCTGC 1 cut(s) 526
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 454
BsrDI GCAATG 2 cut(s) 189, 283
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 659
BssMI GATC 4 cut(s) 49, 141, 229, 369
BssSI CACGAG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 507
Bst4CI ACNGT 5 cut(s) 70, 108, 197, 353, 667
BstBAI YACGTR 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 596
BstC8I GCNNGC 1 cut(s) 661
BstDEI CTNAG 1 cut(s) 10
BstEII GGTNACC 1 cut(s) 102
BstF5I GGATG 2 cut(s) 5, 100
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 4 cut(s) 52, 144, 232, 372
BstMBI GATC 4 cut(s) 49, 141, 229, 369
BstMCI CGRYCG 1 cut(s) 69
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstPI GGTNACC 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 616
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 228, 331
Bsu15I ATCGAT 2 cut(s) 118, 372
BsuRI GGCC 1 cut(s) 284
BsuTUI ATCGAT 2 cut(s) 118, 372
BtsCI GGATG 2 cut(s) 5, 100
BtsIMutI CAGTG 1 cut(s) 663
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 661
CciI TCATGA 2 cut(s) 432, 483
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 175, 465
ClaI ATCGAT 2 cut(s) 118, 372
CseI GACGC 2 cut(s) 323, 642
Csp6I GTAC 5 cut(s) 37, 151, 220, 515, 562
CviAII CATG 3 cut(s) 433, 484, 524
CviJI RGCY 8 cut(s) 14, 241, 284, 344, 428, 499, 604, 637
CviKI_1 RGCY 8 cut(s) 14, 241, 284, 344, 428, 499, 604, 637
CviQI GTAC 5 cut(s) 37, 151, 220, 515, 562
DdeI CTNAG 1 cut(s) 10
DpnI GATC 4 cut(s) 51, 143, 231, 371
DpnII GATC 4 cut(s) 49, 141, 229, 369
Eco47I GGWCC 2 cut(s) 175, 465
Eco88I CYCGRG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 102
EcoO65I GGTNACC 1 cut(s) 102
FaeI CATG 3 cut(s) 436, 487, 527
FaqI GGGAC 1 cut(s) 478
FatI CATG 3 cut(s) 432, 483, 523
Fnu4HI GCNGC 3 cut(s) 242, 282, 345
FokI GGATG 1 cut(s) 107
Fsp4HI GCNGC 3 cut(s) 242, 282, 345
FspBI CTAG 1 cut(s) 318
GlaI GCGC 1 cut(s) 181
GluI GCNGC 3 cut(s) 242, 282, 345
HaeIII GGCC 1 cut(s) 284
HapII CCGG 1 cut(s) 660
HgaI GACGC 2 cut(s) 323, 642
HhaI GCGC 1 cut(s) 182
Hin1II CATG 3 cut(s) 436, 487, 527
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 2 cut(s) 598, 626
HpaII CCGG 1 cut(s) 660
HphI GGTGA 2 cut(s) 263, 379
Hpy166II GTNNAC 2 cut(s) 151, 220
Hpy188III TCNNGA 3 cut(s) 433, 484, 632
Hpy8I GTNNAC 2 cut(s) 151, 220
HpyAV CCTTC 4 cut(s) 79, 317, 358, 445
HpyCH4III ACNGT 5 cut(s) 70, 108, 197, 353, 667
HpyCH4IV ACGT 2 cut(s) 564, 675
HpyCH4V TGCA 2 cut(s) 276, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 2 cut(s) 564, 675
Hsp92II CATG 3 cut(s) 436, 487, 527
HspAI GCGC 1 cut(s) 180
KroI GCCGGC 1 cut(s) 659
KroNI GCCGGC 1 cut(s) 661
Kzo9I GATC 4 cut(s) 49, 141, 229, 369
LpnPI CCDG 6 cut(s) 85, 255, 346, 481, 531, 673
Lsp1109I GCAGC 2 cut(s) 228, 331
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 318
MaeII ACGT 2 cut(s) 564, 675
MaeIII GTNAC 3 cut(s) 102, 251, 356
MalI GATC 4 cut(s) 51, 143, 231, 371
MboI GATC 4 cut(s) 49, 141, 229, 369
MboII GAAGA 3 cut(s) 94, 146, 340
MluCI AATT 2 cut(s) 333, 576
MroNI GCCGGC 1 cut(s) 659
MseI TTAA 4 cut(s) 123, 162, 336, 669
MspA1I CMGCKG 2 cut(s) 29, 241
MspI CCGG 1 cut(s) 660
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 661
NdeII GATC 4 cut(s) 49, 141, 229, 369
NgoMIV GCCGGC 1 cut(s) 659
NlaIII CATG 3 cut(s) 436, 487, 527
NlaIV GGNNCC 2 cut(s) 456, 466
NmuCI GTSAC 1 cut(s) 251
NspV TTCGAA 1 cut(s) 596
PaeR7I CTCGAG 1 cut(s) 632
PagI TCATGA 2 cut(s) 432, 483
PdiI GCCGGC 1 cut(s) 661
PfeI GAWTC 2 cut(s) 598, 626
PkrI GCNGC 3 cut(s) 243, 283, 346
Ppu21I YACGTR 1 cut(s) 565
PsiI TTATAA 2 cut(s) 203, 581
PspEI GGTNACC 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 456, 466
PspPI GGNCC 2 cut(s) 175, 465
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 5 cut(s) 38, 152, 221, 516, 563
RsaNI GTAC 5 cut(s) 37, 151, 220, 515, 562
SaqAI TTAA 4 cut(s) 123, 162, 336, 669
SatI GCNGC 3 cut(s) 242, 282, 345
Sau3AI GATC 4 cut(s) 49, 141, 229, 369
Sau96I GGNCC 2 cut(s) 175, 465
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 616
Sfr274I CTCGAG 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 596
SinI GGWCC 2 cut(s) 175, 465
SlaI CTCGAG 1 cut(s) 632
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 2 cut(s) 333, 576
SsiI CCGC 4 cut(s) 27, 92, 178, 281
SspMI CTAG 1 cut(s) 318
TaaI ACNGT 5 cut(s) 70, 108, 197, 353, 667
TaiI ACGT 2 cut(s) 567, 678
TaqI TCGA 6 cut(s) 65, 118, 372, 596, 612, 633
TasI AATT 2 cut(s) 333, 576
TatI WGTACW 1 cut(s) 150
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 2 cut(s) 598, 626
Tru1I TTAA 4 cut(s) 123, 162, 336, 669
Tru9I TTAA 4 cut(s) 123, 162, 336, 669
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 2 cut(s) 241, 344
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 2 cut(s) 449, 500
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 2 cut(s) 175, 465
XhoI CTCGAG 1 cut(s) 632
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.