pycom13g10990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010108
Physical Location & Seq
Forward (+)
7440008 .. 7441504
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10990.1

Sequence Viewer

Length: 618 bp
ATGACAGCTCCAAACATGGCGACAATCGCTGCTTCTCTGGAGAGGTCTCTCCAAAACTGCTCCCTAAACAACCACCAGAGTGACCACCACAACCGGAGCAGCGGCAACGTCACAGTCGACGGCGAAGGTCAAGGCTCGAGCGTCACTGACGGCGTATTGGCCGCCATGGGGATGCAGGGCAGGTCATCGTCTTCAACCTCATCAACCTCGGACGCCACTTTAGAGCTCAACTCCCATGTCTCCCTTCCATACCATTGGGAACAATGCCTCGATTTAAAGACAGGGGAGATTTACTACATAAACTGGAGGAACGGGATGAAAGTGAAAGAAGATCCGAGGACAAGAGCAGAGTACAGTGGCGACTTCTACTACTCAGAAGAAGATGAAAACAGCTCCTATGACAACGAAGAGTCTTCGACCGACTCGTCGCCATCTTGGTGCAGATACGAGCAGCAGCAGCAGCAGGTAGAGAATCACAACAACAACAATAATGTGTTAGTAGTGGGTGGCTGCAAAGCTTGTCTCATGTACTTCATGGTCCCGAAACAGCTCGAGGACTGCCCCAAATGCTCCGGTCAACTTCTCCACTTCGATCGATCCGAAAATGGCTCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

22.81

Weight (kDa)

4.75

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 424
Acc36I ACCTGC 2 cut(s) 171, 454
AccI GTMKAC 1 cut(s) 117
AciI CCGC 2 cut(s) 102, 162
AclWI GGATC 2 cut(s) 326, 591
AcoI YGGCCR 1 cut(s) 159
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 353, 530
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 1 cut(s) 195
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 5 cut(s) 8, 226, 393, 518, 550
AluI AGCT 5 cut(s) 8, 226, 393, 518, 550
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 3 cut(s) 51, 244, 527
AlwI GGATC 2 cut(s) 326, 591
Ama87I CYCGRG 2 cut(s) 136, 551
AoxI GGCC 1 cut(s) 159
ApeKI GCWGC 7 cut(s) 29, 99, 451, 454, 457, 460, 510
AspS9I GGNCC 1 cut(s) 538
AvaI CYCGRG 2 cut(s) 136, 551
AvaII GGWCC 1 cut(s) 538
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 2 cut(s) 183, 405
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 7 cut(s) 16, 111, 463, 466, 469, 472, 497
BccI CCATC 1 cut(s) 439
BceAI ACGGC 2 cut(s) 136, 166
BcoDI GTCTC 3 cut(s) 51, 244, 527
BfuAI ACCTGC 2 cut(s) 171, 454
BisI GCNGC 9 cut(s) 30, 100, 103, 162, 452, 455, 458, 461, 511
BlsI GCNGC 9 cut(s) 31, 101, 104, 163, 453, 456, 459, 462, 512
Bme18I GGWCC 1 cut(s) 538
BmeT110I CYCGRG 2 cut(s) 136, 551
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 2 cut(s) 540, 610
BmsI GCATC 1 cut(s) 162
BpiI GAAGAC 2 cut(s) 183, 405
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpmI CTGGAG 2 cut(s) 59, 325
Bsa29I ATCGAT 1 cut(s) 595
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 165, 207, 335
BsaWI WCCGGW 2 cut(s) 93, 572
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 1 cut(s) 308
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 3 cut(s) 165, 207, 335
BseGI GGATG 2 cut(s) 177, 321
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 7 cut(s) 16, 111, 463, 466, 469, 472, 497
BsgI GTGCAG 1 cut(s) 460
Bsh1285I CGRYCG 2 cut(s) 420, 595
BshFI GGCC 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 595
BsiEI CGRYCG 2 cut(s) 420, 595
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 2 cut(s) 136, 551
BsiSI CCGG 2 cut(s) 94, 573
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 3 cut(s) 51, 244, 527
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 1 cut(s) 161
Bso31I GGTCTC 1 cut(s) 51
BsoBI CYCGRG 2 cut(s) 136, 551
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 3 cut(s) 331, 592, 596
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 2 cut(s) 102, 162
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 386
BspDI ATCGAT 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 540, 610
BspMI ACCTGC 2 cut(s) 171, 454
BspPI GGATC 2 cut(s) 326, 591
BspTNI GGTCTC 1 cut(s) 51
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 3 cut(s) 165, 207, 335
BssMI GATC 3 cut(s) 331, 592, 596
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 115, 356
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 373
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 177, 321
BstKTI GATC 3 cut(s) 334, 595, 599
BstMAI GTCTC 3 cut(s) 51, 244, 527
BstMBI GATC 3 cut(s) 331, 592, 596
BstMCI CGRYCG 2 cut(s) 420, 595
BstMWI GCNNNNNNNGC 4 cut(s) 26, 457, 460, 567
BstV1I GCAGC 7 cut(s) 16, 111, 463, 466, 469, 472, 497
BstV2I GAAGAC 2 cut(s) 183, 405
BstX2I RGATCY 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 255
BstYI RGATCY 1 cut(s) 331
Bsu15I ATCGAT 1 cut(s) 595
BsuRI GGCC 1 cut(s) 161
BsuTUI ATCGAT 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 2 cut(s) 177, 321
BtsIMutI CAGTG 2 cut(s) 144, 361
BveI ACCTGC 2 cut(s) 171, 454
Cfr13I GGNCC 1 cut(s) 538
ClaI ATCGAT 1 cut(s) 595
CseI GACGC 2 cut(s) 130, 221
Csp6I GTAC 2 cut(s) 352, 529
CviAII CATG 6 cut(s) 16, 166, 236, 526, 535, 615
CviJI RGCY 9 cut(s) 8, 135, 161, 226, 393, 510, 518, 550, 609
CviKI_1 RGCY 9 cut(s) 8, 135, 161, 226, 393, 510, 518, 550, 609
CviQI GTAC 2 cut(s) 352, 529
DdeI CTNAG 1 cut(s) 373
DpnI GATC 3 cut(s) 333, 594, 598
DpnII GATC 3 cut(s) 331, 592, 596
DraI TTTAAA 1 cut(s) 276
DrdI GACNNNNNNGTC 1 cut(s) 424
DseDI GACNNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 226
Eco88I CYCGRG 2 cut(s) 136, 551
EcoICRI GAGCTC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 6 cut(s) 19, 169, 239, 529, 538, 618
FaiI YATR 9 cut(s) 17, 167, 237, 250, 299, 399, 527, 536, 616
FaqI GGGAC 1 cut(s) 524
FatI CATG 6 cut(s) 15, 165, 235, 525, 534, 614
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 9 cut(s) 30, 100, 103, 162, 452, 455, 458, 461, 511
FokI GGATG 2 cut(s) 184, 328
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 9 cut(s) 30, 100, 103, 162, 452, 455, 458, 461, 511
GluI GCNGC 9 cut(s) 30, 100, 103, 162, 452, 455, 458, 461, 511
GsuI CTGGAG 2 cut(s) 59, 325
HaeIII GGCC 1 cut(s) 161
HapII CCGG 2 cut(s) 94, 573
HgaI GACGC 2 cut(s) 130, 221
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 19, 169, 239, 529, 538, 618
HincII GTYRAC 2 cut(s) 118, 578
HindII GTYRAC 2 cut(s) 118, 578
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 3 cut(s) 410, 422, 472
HpaII CCGG 2 cut(s) 94, 573
Hpy166II GTNNAC 2 cut(s) 118, 578
Hpy188I TCNGA 4 cut(s) 211, 336, 376, 601
Hpy188III TCNNGA 2 cut(s) 38, 541
Hpy8I GTNNAC 2 cut(s) 118, 578
Hpy99I CGWCG 2 cut(s) 122, 430
HpyAV CCTTC 2 cut(s) 119, 254
HpyCH4III ACNGT 2 cut(s) 115, 356
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 175, 441, 513
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 457, 460, 567
HpyF3I CTNAG 1 cut(s) 373
HpySE526I ACGT 1 cut(s) 108
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 19, 169, 239, 529, 538, 618
Kzo9I GATC 3 cut(s) 331, 592, 596
LmnI GCTCC 6 cut(s) 13, 65, 96, 398, 575, 614
LpnPI CCDG 9 cut(s) 23, 89, 107, 161, 166, 267, 289, 449, 586
Lsp1109I GCAGC 7 cut(s) 16, 111, 463, 466, 469, 472, 497
LweI GCATC 1 cut(s) 162
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 3 cut(s) 80, 109, 142
MalI GATC 3 cut(s) 333, 594, 598
MboI GATC 3 cut(s) 331, 592, 596
MboII GAAGA 6 cut(s) 183, 341, 389, 392, 405, 419
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 228
MlyI GAGTC 2 cut(s) 416, 419
MnlI CCTC 7 cut(s) 36, 208, 217, 278, 300, 330, 547
MseI TTAA 1 cut(s) 275
MslI CAYNNNNRTG 3 cut(s) 78, 170, 436
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 2 cut(s) 94, 573
MwoI GCNNNNNNNGC 4 cut(s) 26, 457, 460, 567
NcoI CCATGG 1 cut(s) 165
NdeII GATC 3 cut(s) 331, 592, 596
NlaIII CATG 6 cut(s) 19, 169, 239, 529, 538, 618
NlaIV GGNNCC 2 cut(s) 540, 610
NmuCI GTSAC 3 cut(s) 80, 109, 142
OliI CACNNNNGTG 1 cut(s) 78
PaeR7I CTCGAG 2 cut(s) 136, 551
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 422, 597
PfeI GAWTC 1 cut(s) 472
PkrI GCNGC 9 cut(s) 31, 101, 104, 163, 453, 456, 459, 462, 512
Ple19I CGATCG 1 cut(s) 595
PleI GAGTC 2 cut(s) 416, 418
PpsI GAGTC 2 cut(s) 416, 418
Psp124BI GAGCTC 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 540, 610
PspPI GGNCC 1 cut(s) 538
PspXI VCTCGAGB 2 cut(s) 136, 551
PsuI RGATCY 1 cut(s) 331
PvuI CGATCG 1 cut(s) 595
RsaI GTAC 2 cut(s) 353, 530
RsaNI GTAC 2 cut(s) 352, 529
RseI CAYNNNNRTG 3 cut(s) 78, 170, 436
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 9 cut(s) 30, 100, 103, 162, 452, 455, 458, 461, 511
Sau3AI GATC 3 cut(s) 331, 592, 596
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 2 cut(s) 416, 419
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 162
Sfr274I CTCGAG 2 cut(s) 136, 551
SinI GGWCC 1 cut(s) 538
SlaI CTCGAG 2 cut(s) 136, 551
SmiMI CAYNNNNRTG 3 cut(s) 78, 170, 436
SmlI CTYRAG 2 cut(s) 136, 551
SmoI CTYRAG 2 cut(s) 136, 551
SsiI CCGC 2 cut(s) 102, 162
SstI GAGCTC 1 cut(s) 228
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 115, 356
TaiI ACGT 1 cut(s) 111
TaqI TCGA 7 cut(s) 117, 137, 270, 416, 552, 591, 595
TaqII GACCGA 1 cut(s) 434
TatI WGTACW 2 cut(s) 351, 528
TauI GCSGC 2 cut(s) 105, 164
TfiI GAWTC 1 cut(s) 472
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 2 cut(s) 151, 361
TseFI GTSAC 3 cut(s) 80, 109, 142
TseI GCWGC 7 cut(s) 29, 99, 451, 454, 457, 460, 510
Tsp45I GTSAC 3 cut(s) 80, 109, 142
TspDTI ATGAA 3 cut(s) 332, 399, 523
TspRI CASTG 2 cut(s) 151, 361
VpaK11BI GGWCC 1 cut(s) 538
XhoI CTCGAG 2 cut(s) 136, 551
XmiI GTMKAC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.