Rmu_co8435519.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8435519.1
OG0010108
Physical Location & Seq
Reverse (-)
164 .. 553
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8435519.1_g000001.1.cds

Sequence Viewer

Length: 390 bp
atggcgacgatcgctgcatctctggagcagtctctgcaaaactgttccttaaaccaccgccagaccaccatcgccgtcggtggcggaaacggtagccgtagcagtcaagggtcgagtcatgttgggatgagaaggtcgtcgtcaacctcatcaacctcagacgatgcgcagcagaatcaattcatgcccgacaacagcaatgaggacacggctctggagctcaactcccatgtctcgcttccctaccattgggaacaatgcctcgatttaaaggtaacaaacgagtttggtctatcgatctccttgctggacaaaatcggaaacccatttatagttttgggttgttttagtttccatttaaagggttccataaatgcagagaaaacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

13.9

Weight (kDa)

5.62

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 168
AciI CCGC 2 cut(s) 58, 84
AfiI CCNNNNNNNGG 2 cut(s) 249, 361
AluBI AGCT 1 cut(s) 220
AluI AGCT 1 cut(s) 220
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 36, 238
AlwNI CAGNNNCTG 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 14, 169
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 77
BceAI ACGGC 3 cut(s) 59, 81, 225
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BcoDI GTCTC 2 cut(s) 36, 238
BisI GCNGC 2 cut(s) 15, 170
BlsI GCNGC 2 cut(s) 16, 171
BmiI GGNNCC 1 cut(s) 367
BmsI GCATC 2 cut(s) 26, 154
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 2 cut(s) 44, 236
Bsa29I ATCGAT 1 cut(s) 296
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 361
Bse3DI GCAATG 1 cut(s) 205
BseCI ATCGAT 1 cut(s) 296
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 249, 361
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 249, 361
BsmAI GTCTC 2 cut(s) 36, 238
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 2 cut(s) 9, 297
BspACI CCGC 2 cut(s) 58, 84
BspCNI CTCAG 1 cut(s) 170
BspDI ATCGAT 1 cut(s) 296
BspLI GGNNCC 1 cut(s) 367
BsrDI GCAATG 1 cut(s) 205
BssMI GATC 2 cut(s) 9, 297
Bst4CI ACNGT 2 cut(s) 44, 92
BstAPI GCANNNNNTGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 2 cut(s) 12, 300
BstMAI GTCTC 2 cut(s) 36, 238
BstMBI GATC 2 cut(s) 9, 297
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 11, 34
BstV1I GCAGC 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 296
BsuTUI ATCGAT 1 cut(s) 296
BtgZI GCGATG 1 cut(s) 55
BtsCI GGATG 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 296
CviAII CATG 3 cut(s) 119, 184, 230
CviJI RGCY 3 cut(s) 96, 212, 220
CviKI_1 RGCY 3 cut(s) 96, 212, 220
DdeI CTNAG 1 cut(s) 157
DpnI GATC 2 cut(s) 11, 299
DpnII GATC 2 cut(s) 9, 297
DraI TTTAAA 2 cut(s) 270, 360
EciI GGCGGA 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 222
FaeI CATG 3 cut(s) 122, 187, 233
FaiI YATR 6 cut(s) 120, 185, 231, 332, 371, 388
FatI CATG 3 cut(s) 118, 183, 229
Fnu4HI GCNGC 2 cut(s) 15, 170
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 2 cut(s) 15, 170
FspI TGCGCA 1 cut(s) 168
GlaI GCGC 1 cut(s) 168
GluI GCNGC 2 cut(s) 15, 170
GsuI CTGGAG 2 cut(s) 44, 236
HhaI GCGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 122, 187, 233
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HinfI GANTC 2 cut(s) 115, 175
Hpy166II GTNNAC 1 cut(s) 144
Hpy188I TCNGA 2 cut(s) 160, 320
Hpy188III TCNNGA 2 cut(s) 23, 215
Hpy8I GTNNAC 1 cut(s) 144
Hpy99I CGWCG 3 cut(s) 10, 80, 142
HpyAV CCTTC 1 cut(s) 126
HpyCH4III ACNGT 2 cut(s) 44, 92
HpyCH4V TGCA 3 cut(s) 17, 37, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 34
HpyF3I CTNAG 1 cut(s) 157
Hsp92II CATG 3 cut(s) 122, 187, 233
HspAI GCGC 1 cut(s) 167
Kzo9I GATC 2 cut(s) 9, 297
LmnI GCTCC 2 cut(s) 25, 217
LpnPI CCDG 4 cut(s) 8, 74, 200, 293
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 2 cut(s) 26, 154
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 11, 299
MboI GATC 2 cut(s) 9, 297
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 1 cut(s) 179
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 4 cut(s) 157, 166, 196, 272
MroXI GAANNNNTTC 1 cut(s) 179
MseI TTAA 3 cut(s) 50, 269, 359
MwoI GCNNNNNNNGC 2 cut(s) 11, 34
NdeII GATC 2 cut(s) 9, 297
NlaIII CATG 3 cut(s) 122, 187, 233
NlaIV GGNNCC 1 cut(s) 367
NsbI TGCGCA 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 2 cut(s) 16, 171
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Psp124BI GAGCTC 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 34
PvuI CGATCG 1 cut(s) 12
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 3 cut(s) 50, 269, 359
SatI GCNGC 2 cut(s) 15, 170
Sau3AI GATC 2 cut(s) 9, 297
SchI GAGTC 1 cut(s) 124
SduI GDGCHC 1 cut(s) 222
SetI ASST 5 cut(s) 137, 149, 158, 222, 276
SfaNI GCATC 2 cut(s) 26, 154
Sse9I AATT 1 cut(s) 179
SsiI CCGC 2 cut(s) 58, 84
SstI GAGCTC 1 cut(s) 222
TaaI ACNGT 2 cut(s) 44, 92
TaqI TCGA 3 cut(s) 113, 264, 296
TasI AATT 1 cut(s) 179
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 3 cut(s) 50, 269, 359
Tru9I TTAA 3 cut(s) 50, 269, 359
TseI GCWGC 2 cut(s) 14, 169
TspDTI ATGAA 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.