pycom13g11920

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005575
Physical Location & Seq
Forward (+)
8218732 .. 8221743
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
ATGAGATTTCTGGGGTCAGCCAACATGAAGCTTTTCTGGGTTTTGGGGTTTATGGGGTTTGTGTTTTGCAGAACAGATGGAACTTTTATTGGGGATGTGAATGTCGCACAGGAACAAGACCTGCATTATACTGAGAGTAATGTGTATGGCTATGCAACTTATGGTTATGGGTTTTCCAGCAGACCCTTAATGGTGGGTCTCACACTTATCAATGGAGCTGCTGCCAAAGGAGCAGTCTGCCTGGATGGAACATTACCTGCTTACCACATCCACCATGGATACGGGTCAGGAGCAAACAGTTGGCTCATTCAGTTGGAGGGAGGAGGATGGTGTGATACAATCAGAAACTGTGTTTACCGTAAGAAAACTCATCGTGGTTCATCAGCATATATGGAGAAGTATATACCATTTGCAGGGATACTAAGCAACAAAGCAGGAGAAAACCCTGACTTTTACAATTGGAACAGAGTTAAGGTTCGATATTGTGATGGTGCATCTTTCGCCGGTGACAGTCACAATGCGGCTGCCCAATTGCATTTTCGAGGACAACGTATCTGGAAAGCTGCTATGGAAGACTTGATGTCAAGGGGAATGCGCTATGCTAATCAGGCTCTTCTTTCTGGATGTTCTGCTGGTGGGGTTGCAACTGTACTCCACTGTGATGAATTTCGTGGAATGTTCCCTGCAACTACTAGAGTGAAGTGCCTCAGTGATGCTGGACTGTTTCTTGACGTAGTTGATGTATCTGGTAGTCGCACTTTGAGAAGTATGTTTAGAAGAGTGGTTAGTCTGCAGGGCGTGCAAAAGAATCTCCCATGGAGTTGTACCAATCGCCTCCACCCGACTTTGTGCTTTTTCCCCCAGCACCTAATTGCAAGTGTCAAGACTCCCCTTTTTCTGGTCAATGCAGCTTATGATACATGGCAGATCCAGGCCAGTTTAGCTCCACGAACAGCAGACCCTAACGGCCATTGGCATGCATGCACAAAAAATAATGGACGTTGTGCTGCATGGCAGATGAACTTTTTGCAAGGTTTCCGGATTCAAATGCTCAAAGCTGTAAGTGGATTCTCGAGGGCTGGTAAAAATGGTTTGTTCATAAATTCTTGCTTTTCTCATTGCCAGACTGAGAGACAAGATACCTGGTTTTCTCAAAATTCTCCTCTTATTGGAAATAAGGGGATAGCAAAATCAGTCGGAAACTGGTATTTCGATCGGTATAGTATCAAAGCTATAGATTGTCCGTACCCTTGTGACAAGACCTGCCATAATCTGGTGTTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

429

Amino Acids

47.68

Weight (kDa)

9.23

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 61 - 423 3.8e-168 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 129, 265, 1271
AccB7I CCANNNNNTGG 1 cut(s) 1273
AccIII TCCGGA 1 cut(s) 1038
AciI CCGC 1 cut(s) 521
AclWI GGATC 1 cut(s) 922
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 3 cut(s) 665, 1102, 1156
AfaI GTAC 3 cut(s) 651, 826, 1247
AfiI CCNNNNNNNGG 4 cut(s) 413, 898, 1169, 1273
AgsI TTSAA 1 cut(s) 1046
AhdI GACNNNNNGTC 1 cut(s) 580
AjnI CCWGG 3 cut(s) 240, 930, 1142
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 7 cut(s) 31, 218, 563, 911, 944, 1058, 1232
AluI AGCT 7 cut(s) 31, 218, 563, 911, 944, 1058, 1232
Alw26I GTCTC 2 cut(s) 203, 1126
AlwI GGATC 1 cut(s) 922
AlwNI CAGNNNCTG 1 cut(s) 348
Ama87I CYCGRG 1 cut(s) 1072
Aor13HI TCCGGA 1 cut(s) 1038
AoxI GGCC 2 cut(s) 933, 967
ApeKI GCWGC 6 cut(s) 218, 221, 524, 563, 908, 1007
ApoI RAATTY 3 cut(s) 665, 1102, 1156
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 597
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 1072
BbsI GAAGAC 1 cut(s) 579
BbvI GCAGC 6 cut(s) 205, 208, 511, 550, 920, 994
BccI CCATC 4 cut(s) 71, 239, 321, 482
BceAI ACGGC 1 cut(s) 982
BciT130I CCWGG 3 cut(s) 242, 932, 1144
BciVI GTATCC 2 cut(s) 272, 411
BcoDI GTCTC 2 cut(s) 203, 1126
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 2 cut(s) 791, 1233
BfuAI ACCTGC 3 cut(s) 129, 265, 1271
BfuI GTATCC 2 cut(s) 272, 411
BisI GCNGC 7 cut(s) 219, 222, 522, 525, 564, 909, 1008
BlsI GCNGC 7 cut(s) 220, 223, 523, 526, 565, 910, 1009
Bme1390I CCNGG 3 cut(s) 242, 932, 1144
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 1072
BmrFI CCNGG 3 cut(s) 242, 932, 1144
BmsI GCATC 2 cut(s) 503, 703
BpiI GAAGAC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 2 cut(s) 274, 815
BsaWI WCCGGW 1 cut(s) 1038
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 898, 1169, 1273
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 936, 1208
Bse3DI GCAATG 1 cut(s) 1117
BseAI TCCGGA 1 cut(s) 1038
BseBI CCWGG 3 cut(s) 242, 932, 1144
BseDI CCNNGG 2 cut(s) 274, 815
BseGI GGATG 5 cut(s) 100, 250, 267, 332, 629
BseLI CCNNNNNNNGG 4 cut(s) 413, 898, 1169, 1273
BseMI GCAATG 1 cut(s) 1117
BseMII CTCAG 3 cut(s) 123, 721, 1119
BseNI ACTGG 2 cut(s) 936, 1208
BseRI GAGGAG 2 cut(s) 336, 1152
BseXI GCAGC 6 cut(s) 205, 208, 511, 550, 920, 994
BseYI CCCAGC 1 cut(s) 861
Bsh1285I CGRYCG 1 cut(s) 1216
BshFI GGCC 2 cut(s) 935, 969
BsiEI CGRYCG 1 cut(s) 1216
BsiHKCI CYCGRG 1 cut(s) 1072
BsiSI CCGG 2 cut(s) 504, 1039
BslI CCNNNNNNNGG 4 cut(s) 413, 898, 1169, 1273
BsmAI GTCTC 2 cut(s) 203, 1126
BsmI GAATGC 1 cut(s) 597
BsnI GGCC 2 cut(s) 935, 969
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 1072
Bsp13I TCCGGA 1 cut(s) 1038
Bsp143I GATC 2 cut(s) 927, 1213
Bsp19I CCATGG 2 cut(s) 274, 815
BspACI CCGC 1 cut(s) 521
BspANI GGCC 2 cut(s) 935, 969
BspCNI CTCAG 3 cut(s) 124, 720, 1120
BspEI TCCGGA 1 cut(s) 1038
BspMAI CTGCAG 1 cut(s) 795
BspMI ACCTGC 3 cut(s) 129, 265, 1271
BspPI GGATC 1 cut(s) 922
BspQI GCTCTTC 1 cut(s) 618
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 1117
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 2 cut(s) 936, 1208
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 2 cut(s) 274, 815
BssMI GATC 2 cut(s) 927, 1213
BssT1I CCWWGG 2 cut(s) 274, 815
Bst2UI CCWGG 3 cut(s) 242, 932, 1144
Bst4CI ACNGT 7 cut(s) 299, 350, 359, 512, 649, 659, 723
Bst6I CTCTTC 2 cut(s) 618, 772
BstAPI GCANNNNNTGC 1 cut(s) 799
BstC8I GCNNGC 3 cut(s) 800, 978, 982
BstDEI CTNAG 4 cut(s) 132, 422, 707, 1128
BstDSI CCRYGG 2 cut(s) 274, 815
BstF5I GGATG 5 cut(s) 100, 250, 267, 332, 629
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 2 cut(s) 930, 1216
BstMAI GTCTC 2 cut(s) 203, 1126
BstMBI GATC 2 cut(s) 927, 1213
BstMCI CGRYCG 1 cut(s) 1216
BstMWI GCNNNNNNNGC 5 cut(s) 230, 500, 608, 799, 941
BstNI CCWGG 3 cut(s) 242, 932, 1144
BstNSI RCATGY 2 cut(s) 980, 984
BstSCI CCNGG 3 cut(s) 240, 930, 1142
BstSFI CTRYAG 2 cut(s) 791, 1233
BstV1I GCAGC 6 cut(s) 205, 208, 511, 550, 920, 994
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 1 cut(s) 927
BstYI RGATCY 1 cut(s) 927
BsuI GTATCC 2 cut(s) 272, 411
BsuRI GGCC 2 cut(s) 935, 969
BtgI CCRYGG 2 cut(s) 274, 815
BtsCI GGATG 5 cut(s) 100, 250, 267, 332, 629
BtsIMutI CAGTG 2 cut(s) 655, 715
BveI ACCTGC 3 cut(s) 129, 265, 1271
Cac8I GCNNGC 3 cut(s) 800, 978, 982
CaiI CAGNNNCTG 1 cut(s) 348
CfoI GCGC 1 cut(s) 597
Cfr10I RCCGGY 1 cut(s) 503
CsiI ACCWGGT 1 cut(s) 1142
Csp6I GTAC 3 cut(s) 650, 825, 1246
CviAII CATG 7 cut(s) 25, 275, 816, 921, 977, 981, 1011
CviQI GTAC 3 cut(s) 650, 825, 1246
DdeI CTNAG 4 cut(s) 132, 422, 707, 1128
DpnI GATC 2 cut(s) 929, 1215
DpnII GATC 2 cut(s) 927, 1213
DriI GACNNNNNGTC 1 cut(s) 580
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 2 cut(s) 618, 772
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 2 cut(s) 618, 772
Eco130I CCWWGG 2 cut(s) 274, 815
Eco31I GGTCTC 1 cut(s) 203
Eco88I CYCGRG 1 cut(s) 1072
EcoRII CCWGG 3 cut(s) 240, 930, 1142
EcoT14I CCWWGG 2 cut(s) 274, 815
EcoT22I ATGCAT 1 cut(s) 982
ErhI CCWWGG 2 cut(s) 274, 815
FaeI CATG 7 cut(s) 28, 278, 819, 924, 980, 984, 1014
FatI CATG 7 cut(s) 24, 274, 815, 920, 976, 980, 1010
Fnu4HI GCNGC 7 cut(s) 219, 222, 522, 525, 564, 909, 1008
FokI GGATG 5 cut(s) 107, 254, 257, 339, 636
Fsp4HI GCNGC 7 cut(s) 219, 222, 522, 525, 564, 909, 1008
FspBI CTAG 1 cut(s) 693
GlaI GCGC 1 cut(s) 596
GluI GCNGC 7 cut(s) 219, 222, 522, 525, 564, 909, 1008
GsaI CCCAGC 1 cut(s) 865
HaeIII GGCC 2 cut(s) 935, 969
HapII CCGG 2 cut(s) 504, 1039
HhaI GCGC 1 cut(s) 597
Hin1II CATG 7 cut(s) 28, 278, 819, 924, 980, 984, 1014
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 4 cut(s) 808, 886, 1042, 1068
HpaII CCGG 2 cut(s) 504, 1039
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 2 cut(s) 344, 1199
Hpy188III TCNNGA 7 cut(s) 288, 556, 621, 728, 883, 1039, 1072
Hpy8I GTNNAC 1 cut(s) 355
HpyCH4III ACNGT 7 cut(s) 299, 350, 359, 512, 649, 659, 723
HpyCH4IV ACGT 3 cut(s) 550, 732, 1000
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 500, 608, 799, 941
HpyF3I CTNAG 4 cut(s) 132, 422, 707, 1128
HpySE526I ACGT 3 cut(s) 550, 732, 1000
Hsp92II CATG 7 cut(s) 28, 278, 819, 924, 980, 984, 1014
HspAI GCGC 1 cut(s) 595
Kpn2I TCCGGA 1 cut(s) 1038
Kzo9I GATC 2 cut(s) 927, 1213
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 4 cut(s) 215, 230, 290, 949
Lsp1109I GCAGC 6 cut(s) 205, 208, 511, 550, 920, 994
LweI GCATC 2 cut(s) 503, 703
MabI ACCWGGT 1 cut(s) 1142
MaeI CTAG 1 cut(s) 693
MaeII ACGT 3 cut(s) 550, 732, 1000
MaeIII GTNAC 3 cut(s) 506, 512, 1253
MalI GATC 2 cut(s) 929, 1215
MboI GATC 2 cut(s) 927, 1213
MboII GAAGA 3 cut(s) 584, 605, 789
MfeI CAATTG 2 cut(s) 457, 530
MflI RGATCY 1 cut(s) 927
MluCI AATT 6 cut(s) 457, 530, 665, 870, 1102, 1156
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 2 cut(s) 294, 1177
MnlI CCTC 8 cut(s) 310, 314, 317, 536, 716, 845, 1068, 1173
Mph1103I ATGCAT 1 cut(s) 982
MroI TCCGGA 1 cut(s) 1038
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 3 cut(s) 188, 471, 1281
MslI CAYNNNNRTG 2 cut(s) 660, 975
MspI CCGG 2 cut(s) 504, 1039
MspR9I CCNGG 3 cut(s) 242, 932, 1144
MunI CAATTG 2 cut(s) 457, 530
Mva1269I GAATGC 1 cut(s) 597
MvaI CCWGG 3 cut(s) 242, 932, 1144
MwoI GCNNNNNNNGC 5 cut(s) 230, 500, 608, 799, 941
NcoI CCATGG 2 cut(s) 274, 815
NdeII GATC 2 cut(s) 927, 1213
NlaIII CATG 7 cut(s) 28, 278, 819, 924, 980, 984, 1014
NmuCI GTSAC 3 cut(s) 506, 512, 1253
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 2 cut(s) 980, 984
PaeI GCATGC 2 cut(s) 980, 984
PaeR7I CTCGAG 1 cut(s) 1072
PciSI GCTCTTC 1 cut(s) 618
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PctI GAATGC 1 cut(s) 597
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 3 cut(s) 808, 1042, 1068
PflMI CCANNNNNTGG 1 cut(s) 1273
PkrI GCNGC 7 cut(s) 220, 223, 523, 526, 565, 910, 1009
Ple19I CGATCG 1 cut(s) 1216
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
Psp6I CCWGG 3 cut(s) 240, 930, 1142
PspFI CCCAGC 1 cut(s) 861
PspGI CCWGG 3 cut(s) 240, 930, 1142
PstI CTGCAG 1 cut(s) 795
PstNI CAGNNNCTG 1 cut(s) 348
PsuI RGATCY 1 cut(s) 927
PvuI CGATCG 1 cut(s) 1216
RsaI GTAC 3 cut(s) 651, 826, 1247
RsaNI GTAC 3 cut(s) 650, 825, 1246
RseI CAYNNNNRTG 2 cut(s) 660, 975
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 3 cut(s) 188, 471, 1281
SatI GCNGC 7 cut(s) 219, 222, 522, 525, 564, 909, 1008
Sau3AI GATC 2 cut(s) 927, 1213
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 3 cut(s) 242, 932, 1144
SexAI ACCWGGT 1 cut(s) 1142
SfaNI GCATC 2 cut(s) 503, 703
SfcI CTRYAG 2 cut(s) 791, 1233
Sfr274I CTCGAG 1 cut(s) 1072
SgrAI CRCCGGYG 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 1072
SmiMI CAYNNNNRTG 2 cut(s) 660, 975
SmlI CTYRAG 1 cut(s) 1072
SmoI CTYRAG 1 cut(s) 1072
SphI GCATGC 2 cut(s) 980, 984
Sse9I AATT 6 cut(s) 457, 530, 665, 870, 1102, 1156
SsiI CCGC 1 cut(s) 521
SspMI CTAG 1 cut(s) 693
StyD4I CCNGG 3 cut(s) 240, 930, 1142
StyI CCWWGG 2 cut(s) 274, 815
TaaI ACNGT 7 cut(s) 299, 350, 359, 512, 649, 659, 723
TaiI ACGT 3 cut(s) 553, 735, 1003
TaqI TCGA 4 cut(s) 478, 541, 1073, 1212
TasI AATT 6 cut(s) 457, 530, 665, 870, 1102, 1156
TatI WGTACW 1 cut(s) 649
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 3 cut(s) 808, 1042, 1068
Tru1I TTAA 3 cut(s) 188, 471, 1281
Tru9I TTAA 3 cut(s) 188, 471, 1281
TscAI CASTG 2 cut(s) 662, 715
TseFI GTSAC 3 cut(s) 506, 512, 1253
TseI GCWGC 6 cut(s) 218, 221, 524, 563, 908, 1007
Tsp45I GTSAC 3 cut(s) 506, 512, 1253
TspDTI ATGAA 5 cut(s) 41, 369, 678, 1034, 1087
TspGWI ACGGA 1 cut(s) 1233
TspRI CASTG 2 cut(s) 662, 715
Van91I CCANNNNNTGG 1 cut(s) 1273
XapI RAATTY 3 cut(s) 665, 1102, 1156
XceI RCATGY 2 cut(s) 980, 984
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.