Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0008859

Physical Location & Seq

Genomic Coordinates Reverse (-)

11163438 .. 11166721

Gene Structure 3284 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGCATCAATGGTGGCCAAGGGCAGTAGTAGTTGCACCACCCAAGTCTCTTCCTCAATAGCTGTTCAAGAAAAGGGAAGTAGGAATAAGAGGAAGTTCCGGGCTGATCCGCCTTTAGGTGACCCGAATAAAATTATTCCTTTATCTCAGACTGAATGCACAAGCTATGAGTTTTCAGCTGAAAAATTCGAAATCAGACAGAGTCATGGACAAATTGGTGTCTGTGACCTGTGCAGTGTTAATCAAGACCATTCTGATGGGTTGAAATTTGACCTTGGATTGTCTAGTGCTGTTGTACCATCCGAGGGTAGCCCAAGCCGGCCCAGAGAGGAACTAGAGGCAGATGAGTTTCAGGATGCCGATTGGAGTGACCTCACAGAAACCCAACTTGAAGGGCTTGTTCTAAGCAATTTGGACATGATTTTCAAAAGTGCAATAAAAAAGATTGTTGCTTGCGGTTACACTGAAGAAGTTGCTGCGAAGGCTGTTTTGCGGTCTGGTCTTTGTTATGGGTGTAAAGACATCGTGTCTAATATAGTGGATAATACTTTAGTTTATCTAAGAAGTGGCCAAGAAATTGATCCTTCGAGGGATCACTGTTTTGAAGATCTACAGCAGCTGGAGAAGTACATATTGGCAGAACTGGTTTGTGTGCTTAGAGAGGTTAGGCCTTTTTTCAGCACTGGTGATGCAATGTGTTGCTTGTTGATTTGTGACATGAATGTATCTCATGCTTGTGCAATGGATGGTGATCCCTTGAATAGTTTCATTAGTGATGGAGCTTCAAATGGAAGTTCCTCCGTCCCGAATCAACCCCAGTCAAAAACAGAAACCAAAAGTTCGGAATTGAATCTTCTGAATCCTTCCAAGCCAGTTCCTTCAGTTCCTGGTTCTCATAGTTCACAGTCTGAGACACCTACGATTGCAGGTGGGGTTTCAAACATGGCTAAACTGAAAAATTCCCTTGTTCGTAGTGGATCGTTGTCTGAGAAAGAGGGTGCACAGTACACATCTGAGAATGGGGATAAATCTTTTGGTGCATCTGGAACATTTCAATCTCCTGTGGTAGAGGAAAAGCTTTTAAGTAGTCGAAAGGTTCATTCTGTTACCACCAAGAGAGAATACATGCTTCGGCACAAGTCACTACACTTGGAAAAAAGTTACCGGACTTATGGATGTAAAGGGTCTTCAAGAACAGGGAAGCTGAGTGGTTTGGGTGGTTTAATCTTGGATAAGAAACTAAAATCTGTGTCAGACTCTACCGCTGTTAATTTGAAGAATGCTTCCTTGAAGATAAGCAAGGCTATGGGAGTTGATGTGCCTCAAGAAAGTGGAAACAATAATCTTTCTGCCAATGCCGGGCCGTCTTCCCCTAGAGCATTTAACCTGGATGTTGAAAATACTGCTTCTGTTTTGCCTAAGAACAGTGTCCCGTCCATGTTACCTGCTGTTTGTGAAACGGCATTACCTGCTGTCGGTACTTCGACTGCATTACCTTCTGTCAATACTTCAACGGCATTACCTGTAGTCAATACTGCAGCTGCATTACCTTCAGCAAATACTACGCCTGCATTATCAGTTGCTGATACTGAGCTTTCCCTTTCTCTGCATCCAAAAAGCATTTCCAATCCAGTTCCTATTAGCTGTCACTCTGATGCAACTAATTCTGTTTTTGCTGGGATACCGTATGATAAATCCTTGGGGCAGTGGGTCCCCCGGGACAAGAAAGATGAAATGATTTTAAAGTTGGTTCCGAGGGCACGGGACTTGCAAAATCAGCTCCAAGAGTGGACAGAGTGGGCAAATCAGAAGGTTATGCAGGCTGCGCGTAGATTGAGCAAGGACAAGGCTGAACTTAAGTCATTGAGGCAAGAGAAAGAAGAAGTTGAGCGGCTGAAGAAAGAGAAGCAAACGCTGGAAGAAAACACCATGAAGAAGCTTTCTGAGATGGAAAATGCTCTCTGCAAGGCTAGTAGCCAGGTTGAACGGGCAAATTCTTCCGTCCGGAGGCTTGAGGTGGAGAATGCTGCCTTGAGGCAGGATATGGAGGCTGCTAAAGTACGTGCTGCAGAATCTGCTGCAAGCTGTCAGGAGGTATCTAAAAGGGAGAAGAAGACACTGATGAAGTTTCAGTCATGGGAGAAGCAGAAAACCATGTTCAGTGAAGAACTTGCGACTGAAAAACGCAAGTTAAAACAGCTGCTACAGGAACTAGAACAAGCAAAAGATCTCCAGGAACAATTGGAGGCTAGATGGCAACAGGAAGAAAAATCAAAGGCAGAAGTACTTGAGCAGGTCAGTTCGATAAAAAAAGAACGGGAACAGATAGAGGCTTCAACAAAATCCAAGGAGGATATGATTAAACTGAAAGCAGAAAACAATCTGCAGAAGTACAAAGATGATATTCAGAAACTCGAAAAAGAAATCTCTCAATTGAGACACAAGAGTGATTCTTCAAAAATTGCTGCGCTGCGGAGGGGCATTGATGGGAGCTACGCCAGCAAAGTAACAGACATCGAAAATGGCCTGGATCACAAGGGGTCCCGGATGCCATACATCTCGGAAGTAGTGAAGGATATTCAAGACTACTCTGAGACAGGAGGGGTGAAACGTGAACGGGAGTGTGTGATGTGCCTCTCTGAGGAGATGTCCGTTGTCTTCCTCCCGTGTGCCCATCAAGTGGTTTGTAGAACATGCAATGAGCTCCACGAGAAACAGGGCATGAAGGATTGCCCGTCTTGTAGGAGCGCGATACAGTGGCGAATTTCTGTACGTTATGCTCGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

930

Amino Acids

102.27

Weight (kDa)

7.15

Isoelectric Point (pI)

52.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIR2-like_helical	PF20235	137 - 249	2.8e-30	PIR2-like, helical domain
zf-C3HC4_3	PF13920	872 - 919	1.7e-11	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008859)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g16660 FvH4_4g16660 FvH4_4g16660 FvH4_4g16660
malus_domestica	MD13G1177600.v1.1 MD16G1179600.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1 Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15320
rosa_chinensis	RchiOBHm_Chr4g0420121
rosa_laevigata	RLG00000007787
rosa_multiflora	Rmu_sc0015433.1_g000001 Rmu_sc0023895.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362830
rosa_rugosa	Rorug04G0162300 Rorug04G0162400
rosa_samantha	Rh4AG223200 Rh4BG225600 Rh4CG236400 Rh4DG222000
rosa_wichuraiana	Rw4G019210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	917
Acc36I	ACCTGC	4 cut(s)	917, 1453, 1477, 2284
AccB7I	CCANNNNNTGG	1 cut(s)	2680
AccBSI	CCGCTC	1 cut(s)	1891
AccII	CGCG	2 cut(s)	1828, 2750
AccIII	TCCGGA	1 cut(s)	2004
AciI	CCGC	6 cut(s)	110, 456, 493, 1263, 1891, 2473
AclWI	GGATC	6 cut(s)	101, 575, 600, 746, 985, 2538
AcoI	YGGCCR	2 cut(s)	15, 568
AcsI	RAATTY	5 cut(s)	185, 266, 958, 1993, 2763
AcuI	CTGAAG	4 cut(s)	486, 864, 1536, 1916
AfaI	GTAC	8 cut(s)	297, 629, 1007, 1480, 2061, 2286, 2393, 2772
AfiI	CCNNNNNNNGG	7 cut(s)	116, 305, 1359, 1475, 2007, 2641, 2680
AflII	CTTAAG	1 cut(s)	1856
AhdI	GACNNNNNGTC	1 cut(s)	526
AjnI	CCWGG	5 cut(s)	886, 1386, 1977, 2232, 2526
AjuI	GAANNNNNNNTTGG	4 cut(s)	617, 649, 1606, 1638
AleI	CACNNNNGTG	1 cut(s)	2445
Alw21I	GWGCWC	2 cut(s)	1003, 2706
Alw26I	GTCTC	4 cut(s)	52, 905, 2431, 2588
Alw44I	GTGCAC	1 cut(s)	999
AlwI	GGATC	6 cut(s)	101, 575, 600, 746, 985, 2538
AlwNI	CAGNNNCTG	4 cut(s)	619, 887, 1583, 2075
Ama87I	CYCGRG	1 cut(s)	1716
Aor13HI	TCCGGA	1 cut(s)	2004
AoxI	GGCC	6 cut(s)	15, 320, 568, 668, 1361, 2524
ApaLI	GTGCAC	1 cut(s)	999
ApoI	RAATTY	5 cut(s)	185, 266, 958, 1993, 2763
Asp700I	GAANNNNTTC	1 cut(s)	764
AspLEI	GCGC	3 cut(s)	1828, 2470, 2750
AspS9I	GGNCC	4 cut(s)	321, 1361, 1711, 2541
AsuC2I	CCSGG	5 cut(s)	101, 1360, 1717, 1718, 2545
AsuHPI	GGTGA	4 cut(s)	131, 698, 761, 2617
AsuII	TTCGAA	1 cut(s)	189
AvaI	CYCGRG	1 cut(s)	1716
AvaII	GGWCC	2 cut(s)	1711, 2541
BaeGI	GKGCMC	3 cut(s)	1003, 1762, 2674
BalI	TGGCCA	2 cut(s)	17, 570
BanII	GRGCYC	1 cut(s)	2706
BarI	GAAGNNNNNNTAC	2 cut(s)	1171, 1203
BauI	CACGAG	1 cut(s)	2708
BbsI	GAAGAC	4 cut(s)	1179, 1359, 2120, 2650
Bbv12I	GWGCWC	2 cut(s)	1003, 2706
BccI	CCATC	8 cut(s)	251, 307, 740, 770, 1942, 2247, 2480, 2682
BceAI	ACGGC	3 cut(s)	1348, 1476, 1530
BciT130I	CCWGG	5 cut(s)	888, 1388, 1979, 2234, 2528
BciVI	GTATCC	1 cut(s)	1674
BcnI	CCSGG	5 cut(s)	101, 1360, 1717, 1718, 2545
BcoDI	GTCTC	4 cut(s)	52, 905, 2431, 2588
BfaI	CTAG	6 cut(s)	285, 335, 1374, 1971, 2213, 2250
BfmI	CTRYAG	6 cut(s)	611, 1524, 1536, 2067, 2204, 2384
BfrI	CTTAAG	1 cut(s)	1856
BfuAI	ACCTGC	4 cut(s)	917, 1453, 1477, 2284
BfuI	GTATCC	1 cut(s)	1674
BglII	AGATCT	2 cut(s)	607, 2227
BmcAI	AGTACT	1 cut(s)	2286
Bme18I	GGWCC	2 cut(s)	1711, 2541
BmeRI	GACNNNNNGTC	1 cut(s)	526
BmeT110I	CYCGRG	1 cut(s)	1716
BmgT120I	GGNCC	4 cut(s)	321, 1361, 1711, 2541
BmiI	GGNNCC	5 cut(s)	1712, 1713, 1752, 2542, 2543
BmrI	ACTGGG	1 cut(s)	811
BmsI	GCATC	7 cut(s)	14, 346, 679, 1049, 1618, 1645, 2538
BmuI	ACTGGG	1 cut(s)	811
BpiI	GAAGAC	4 cut(s)	1179, 1359, 2120, 2650
BpmI	CTGGAG	2 cut(s)	641, 2216
Bpu14I	TTCGAA	1 cut(s)	189
BpuEI	CTTGAG	4 cut(s)	1308, 2033, 2053, 2309
BpuMI	CCSGG	5 cut(s)	101, 1360, 1717, 1718, 2545
BsaAI	YACGTR	1 cut(s)	2063
BsaBI	GATNNNNATC	1 cut(s)	750
BsaJI	CCNNGG	8 cut(s)	18, 274, 303, 1698, 1715, 1716, 1754, 2346
BsaWI	WCCGGW	2 cut(s)	1164, 2004
BsaXI	ACNNNNNCTCC	2 cut(s)	1302, 1332
Bsc4I	CCNNNNNNNGG	7 cut(s)	116, 305, 1359, 1475, 2007, 2641, 2680
Bse118I	RCCGGY	1 cut(s)	318
Bse1I	ACTGG	5 cut(s)	648, 688, 817, 872, 1631
Bse3DI	GCAATG	3 cut(s)	699, 747, 2704
Bse8I	GATNNNNATC	1 cut(s)	750
BseAI	TCCGGA	1 cut(s)	2004
BseBI	CCWGG	5 cut(s)	888, 1388, 1979, 2234, 2528
BseDI	CCNNGG	8 cut(s)	18, 274, 303, 1698, 1715, 1716, 1754, 2346
BseGI	GGATG	7 cut(s)	299, 361, 751, 1181, 1396, 1609, 2553
BseJI	GATNNNNATC	1 cut(s)	750
BseLI	CCNNNNNNNGG	7 cut(s)	116, 305, 1359, 1475, 2007, 2641, 2680
BseMI	GCAATG	3 cut(s)	699, 747, 2704
BseMII	CTCAG	9 cut(s)	161, 900, 978, 1005, 1196, 1581, 1935, 2583, 2631
BseNI	ACTGG	5 cut(s)	648, 688, 817, 872, 1631
BseRI	GAGGAG	1 cut(s)	2657
BseSI	GKGCMC	3 cut(s)	1003, 1762, 2674
BseYI	CCCAGC	1 cut(s)	1676
BsgI	GTGCAG	1 cut(s)	253
Bsh1236I	CGCG	2 cut(s)	1828, 2750
BshFI	GGCC	6 cut(s)	17, 322, 570, 670, 1363, 2526
BsiHKAI	GWGCWC	2 cut(s)	1003, 2706
BsiHKCI	CYCGRG	1 cut(s)	1716
BsiSI	CCGG	7 cut(s)	100, 319, 1165, 1359, 1717, 2005, 2545
BslFI	GGGAC	6 cut(s)	788, 1415, 1697, 1733, 1778, 2527
BslI	CCNNNNNNNGG	7 cut(s)	116, 305, 1359, 1475, 2007, 2641, 2680
BsmAI	GTCTC	4 cut(s)	52, 905, 2431, 2588
BsmFI	GGGAC	6 cut(s)	788, 1415, 1697, 1733, 1778, 2527
BsmI	GAATGC	3 cut(s)	161, 1285, 2029
BsnI	GGCC	6 cut(s)	17, 322, 570, 670, 1363, 2526
BsoBI	CYCGRG	1 cut(s)	1716
Bsp119I	TTCGAA	1 cut(s)	189
Bsp1286I	GDGCHC	4 cut(s)	1003, 1762, 2674, 2706
Bsp13I	TCCGGA	1 cut(s)	2004
Bsp143I	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
BspACI	CCGC	6 cut(s)	110, 456, 493, 1263, 1891, 2473
BspANI	GGCC	6 cut(s)	17, 322, 570, 670, 1363, 2526
BspCNI	CTCAG	9 cut(s)	160, 901, 979, 1006, 1197, 1582, 1936, 2584, 2632
BspEI	TCCGGA	1 cut(s)	2004
BspFNI	CGCG	2 cut(s)	1828, 2750
BspLI	GGNNCC	5 cut(s)	1712, 1713, 1752, 2542, 2543
BspMAI	CTGCAG	3 cut(s)	1540, 2071, 2388
BspMI	ACCTGC	4 cut(s)	917, 1453, 1477, 2284
BspPI	GGATC	6 cut(s)	101, 575, 600, 746, 985, 2538
BspT104I	TTCGAA	1 cut(s)	189
BspTI	CTTAAG	1 cut(s)	1856
BsrBI	CCGCTC	1 cut(s)	1891
BsrDI	GCAATG	3 cut(s)	699, 747, 2704
BsrFI	RCCGGY	1 cut(s)	318
BsrI	ACTGG	5 cut(s)	648, 688, 817, 872, 1631
BssAI	RCCGGY	1 cut(s)	318
BssECI	CCNNGG	8 cut(s)	18, 274, 303, 1698, 1715, 1716, 1754, 2346
BssMI	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
BssSI	CACGAG	1 cut(s)	2708
BssT1I	CCWWGG	4 cut(s)	18, 274, 1698, 2346
Bst2BI	CACGAG	1 cut(s)	2708
Bst2UI	CCWGG	5 cut(s)	888, 1388, 1979, 2234, 2528
Bst4CI	ACNGT	6 cut(s)	599, 906, 1005, 1427, 1686, 2757
Bst6I	CTCTTC	1 cut(s)	55
BstAFI	CTTAAG	1 cut(s)	1856
BstAPI	GCANNNNNTGC	2 cut(s)	1469, 2075
BstBAI	YACGTR	1 cut(s)	2063
BstBI	TTCGAA	1 cut(s)	189
BstC8I	GCNNGC	6 cut(s)	320, 454, 1569, 1821, 2083, 2500
BstEII	GGTNACC	1 cut(s)	119
BstENI	CCTNNNNNAGG	1 cut(s)	2639
BstF5I	GGATG	7 cut(s)	299, 361, 751, 1181, 1396, 1609, 2553
BstFNI	CGCG	2 cut(s)	1828, 2750
BstHHI	GCGC	3 cut(s)	1828, 2470, 2750
BstKTI	GATC	8 cut(s)	109, 583, 595, 610, 754, 980, 2230, 2533
BstMAI	GTCTC	4 cut(s)	52, 905, 2431, 2588
BstMBI	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
BstMWI	GCNNNNNNNGC	6 cut(s)	482, 1469, 1777, 1825, 2075, 2499
BstNI	CCWGG	5 cut(s)	888, 1388, 1979, 2234, 2528
BstNSI	RCATGY	2 cut(s)	1129, 2697
BstPI	GGTNACC	1 cut(s)	119
BstSFI	CTRYAG	6 cut(s)	611, 1524, 1536, 2067, 2204, 2384
BstSLI	GKGCMC	3 cut(s)	1003, 1762, 2674
BstUI	CGCG	2 cut(s)	1828, 2750
BstV2I	GAAGAC	4 cut(s)	1179, 1359, 2120, 2650
BstX2I	RGATCY	2 cut(s)	607, 2227
BstXI	CCANNNNNNTGG	1 cut(s)	257
BstYI	RGATCY	2 cut(s)	607, 2227
BsuI	GTATCC	1 cut(s)	1674
BsuRI	GGCC	6 cut(s)	17, 322, 570, 670, 1363, 2526
BtsCI	GGATG	7 cut(s)	299, 361, 751, 1181, 1396, 1609, 2553
BtsI	GCAGTG	2 cut(s)	241, 1712
BtsIMutI	CAGTG	9 cut(s)	241, 462, 595, 681, 1432, 1712, 2117, 2167, 2762
BveI	ACCTGC	4 cut(s)	917, 1453, 1477, 2284
Cac8I	GCNNGC	6 cut(s)	320, 454, 1569, 1821, 2083, 2500
CaiI	CAGNNNCTG	4 cut(s)	619, 887, 1583, 2075
CfoI	GCGC	3 cut(s)	1828, 2470, 2750
Cfr10I	RCCGGY	1 cut(s)	318
Cfr13I	GGNCC	4 cut(s)	321, 1361, 1711, 2541
Cfr9I	CCCGGG	1 cut(s)	1716
Csp6I	GTAC	8 cut(s)	296, 628, 1006, 1479, 2060, 2285, 2392, 2771
CviQI	GTAC	8 cut(s)	296, 628, 1006, 1479, 2060, 2285, 2392, 2771
DpnI	GATC	8 cut(s)	108, 582, 594, 609, 753, 979, 2229, 2532
DpnII	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
DraI	TTTAAA	1 cut(s)	1743
DriI	GACNNNNNGTC	1 cut(s)	526
EaeI	YGGCCR	2 cut(s)	15, 568
Eam1104I	CTCTTC	1 cut(s)	55
Eam1105I	GACNNNNNGTC	1 cut(s)	526
EarI	CTCTTC	1 cut(s)	55
EciI	GGCGGA	1 cut(s)	99
Ecl136II	GAGCTC	1 cut(s)	2704
Eco130I	CCWWGG	4 cut(s)	18, 274, 1698, 2346
Eco147I	AGGCCT	1 cut(s)	670
Eco24I	GRGCYC	1 cut(s)	2706
Eco47I	GGWCC	2 cut(s)	1711, 2541
Eco53kI	GAGCTC	1 cut(s)	2704
Eco57I	CTGAAG	4 cut(s)	486, 864, 1536, 1916
Eco88I	CYCGRG	1 cut(s)	1716
Eco91I	GGTNACC	1 cut(s)	119
EcoICRI	GAGCTC	1 cut(s)	2704
EcoNI	CCTNNNNNAGG	1 cut(s)	2639
EcoO109I	RGGNCCY	2 cut(s)	1711, 2541
EcoO65I	GGTNACC	1 cut(s)	119
EcoRII	CCWGG	5 cut(s)	886, 1386, 1977, 2232, 2526
EcoT14I	CCWWGG	4 cut(s)	18, 274, 1698, 2346
EcoT38I	GRGCYC	1 cut(s)	2706
ErhI	CCWWGG	4 cut(s)	18, 274, 1698, 2346
FaqI	GGGAC	6 cut(s)	788, 1415, 1697, 1733, 1778, 2527
FokI	GGATG	7 cut(s)	286, 368, 758, 1188, 1403, 1596, 2560
FriOI	GRGCYC	1 cut(s)	2706
FspBI	CTAG	6 cut(s)	285, 335, 1374, 1971, 2213, 2250
GlaI	GCGC	3 cut(s)	1827, 2469, 2749
GsaI	CCCAGC	1 cut(s)	1680
GsuI	CTGGAG	2 cut(s)	641, 2216
HaeIII	GGCC	6 cut(s)	17, 322, 570, 670, 1363, 2526
HapII	CCGG	7 cut(s)	100, 319, 1165, 1359, 1717, 2005, 2545
HhaI	GCGC	3 cut(s)	1828, 2470, 2750
Hin6I	GCGC	3 cut(s)	1826, 2468, 2748
HinP1I	GCGC	3 cut(s)	1826, 2468, 2748
HindIII	AAGCTT	2 cut(s)	1076, 1937
HinfI	GANTC	7 cut(s)	202, 808, 850, 859, 1256, 2072, 2450
HpaII	CCGG	7 cut(s)	100, 319, 1165, 1359, 1717, 2005, 2545
HphI	GGTGA	4 cut(s)	131, 698, 761, 2617
Hpy166II	GTNNAC	5 cut(s)	902, 1001, 1008, 1791, 2615
Hpy8I	GTNNAC	5 cut(s)	902, 1001, 1008, 1791, 2615
HpyCH4III	ACNGT	6 cut(s)	599, 906, 1005, 1427, 1686, 2757
HpyCH4IV	ACGT	3 cut(s)	2062, 2611, 2773
HpyF10VI	GCNNNNNNNGC	6 cut(s)	482, 1469, 1777, 1825, 2075, 2499
HpySE526I	ACGT	3 cut(s)	2062, 2611, 2773
HspAI	GCGC	3 cut(s)	1826, 2468, 2748
KflI	GGGWCCC	2 cut(s)	1711, 2541
Kpn2I	TCCGGA	1 cut(s)	2004
KroI	GCCGGC	1 cut(s)	318
KroNI	GCCGGC	1 cut(s)	320
Kzo9I	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
LmnI	GCTCC	5 cut(s)	779, 1785, 2490, 2709, 2745
LweI	GCATC	7 cut(s)	14, 346, 679, 1049, 1618, 1645, 2538
MaeI	CTAG	6 cut(s)	285, 335, 1374, 1971, 2213, 2250
MaeII	ACGT	3 cut(s)	2062, 2611, 2773
MalI	GATC	8 cut(s)	108, 582, 594, 609, 753, 979, 2229, 2532
MbiI	CCGCTC	1 cut(s)	1891
MboI	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
MfeI	CAATTG	2 cut(s)	2240, 2432
MflI	RGATCY	2 cut(s)	607, 2227
MhlI	GDGCHC	4 cut(s)	1003, 1762, 2674, 2706
MlsI	TGGCCA	2 cut(s)	17, 570
MluNI	TGGCCA	2 cut(s)	17, 570
MlyI	GAGTC	2 cut(s)	211, 1250
Mox20I	TGGCCA	2 cut(s)	17, 570
MroI	TCCGGA	1 cut(s)	2004
MroNI	GCCGGC	1 cut(s)	318
MroXI	GAANNNNTTC	1 cut(s)	764
MscI	TGGCCA	2 cut(s)	17, 570
MslI	CAYNNNNRTG	4 cut(s)	255, 735, 1653, 2445
Msp20I	TGGCCA	2 cut(s)	17, 570
MspA1I	CMGCKG	5 cut(s)	179, 619, 1265, 1541, 2200
MspCI	CTTAAG	1 cut(s)	1856
MspI	CCGG	7 cut(s)	100, 319, 1165, 1359, 1717, 2005, 2545
MunI	CAATTG	2 cut(s)	2240, 2432
Mva1269I	GAATGC	3 cut(s)	161, 1285, 2029
MvaI	CCWGG	5 cut(s)	888, 1388, 1979, 2234, 2528
MvnI	CGCG	2 cut(s)	1828, 2750
MwoI	GCNNNNNNNGC	6 cut(s)	482, 1469, 1777, 1825, 2075, 2499
NaeI	GCCGGC	1 cut(s)	320
NciI	CCSGG	5 cut(s)	101, 1360, 1717, 1718, 2545
NdeII	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
NgoMIV	GCCGGC	1 cut(s)	318
NlaIV	GGNNCC	5 cut(s)	1712, 1713, 1752, 2542, 2543
NmuCI	GTSAC	6 cut(s)	119, 224, 368, 713, 1140, 1646
NspI	RCATGY	2 cut(s)	1129, 2697
NspV	TTCGAA	1 cut(s)	189
OliI	CACNNNNGTG	1 cut(s)	2445
PaqCI	CACCTGC	1 cut(s)	917
PceI	AGGCCT	1 cut(s)	670
PcsI	WCGNNNNNNNCGW	1 cut(s)	2779
PctI	GAATGC	3 cut(s)	161, 1285, 2029
PdiI	GCCGGC	1 cut(s)	320
PdmI	GAANNNNTTC	1 cut(s)	764
PfeI	GAWTC	5 cut(s)	808, 850, 859, 2072, 2450
PflFI	GACNNNGTC	1 cut(s)	201
PflMI	CCANNNNNTGG	1 cut(s)	2680
PfoI	TCCNGGA	2 cut(s)	2232, 2543
PleI	GAGTC	2 cut(s)	210, 1250
PpsI	GAGTC	2 cut(s)	210, 1250
Ppu21I	YACGTR	1 cut(s)	2063
PpuMI	RGGWCCY	2 cut(s)	1711, 2541
Psp124BI	GAGCTC	1 cut(s)	2706
Psp5II	RGGWCCY	2 cut(s)	1711, 2541
Psp6I	CCWGG	5 cut(s)	886, 1386, 1977, 2232, 2526
PspEI	GGTNACC	1 cut(s)	119
PspFI	CCCAGC	1 cut(s)	1676
PspGI	CCWGG	5 cut(s)	886, 1386, 1977, 2232, 2526
PspN4I	GGNNCC	5 cut(s)	1712, 1713, 1752, 2542, 2543
PspPI	GGNCC	4 cut(s)	321, 1361, 1711, 2541
PspPPI	RGGWCCY	2 cut(s)	1711, 2541
PstI	CTGCAG	3 cut(s)	1540, 2071, 2388
PstNI	CAGNNNCTG	4 cut(s)	619, 887, 1583, 2075
PsuI	RGATCY	2 cut(s)	607, 2227
PsyI	GACNNNGTC	1 cut(s)	201
PvuII	CAGCTG	4 cut(s)	179, 619, 1541, 2200
RsaI	GTAC	8 cut(s)	297, 629, 1007, 1480, 2061, 2286, 2393, 2772
RsaNI	GTAC	8 cut(s)	296, 628, 1006, 1479, 2060, 2285, 2392, 2771
RseI	CAYNNNNRTG	4 cut(s)	255, 735, 1653, 2445
SacI	GAGCTC	1 cut(s)	2706
Sau3AI	GATC	8 cut(s)	106, 580, 592, 607, 751, 977, 2227, 2530
Sau96I	GGNCC	4 cut(s)	321, 1361, 1711, 2541
ScaI	AGTACT	1 cut(s)	2286
SchI	GAGTC	2 cut(s)	211, 1250
SduI	GDGCHC	4 cut(s)	1003, 1762, 2674, 2706
SfaNI	GCATC	7 cut(s)	14, 346, 679, 1049, 1618, 1645, 2538
SfcI	CTRYAG	6 cut(s)	611, 1524, 1536, 2067, 2204, 2384
SfuI	TTCGAA	1 cut(s)	189
SinI	GGWCC	2 cut(s)	1711, 2541
SmaI	CCCGGG	1 cut(s)	1718
SmiMI	CAYNNNNRTG	4 cut(s)	255, 735, 1653, 2445
SmlI	CTYRAG	5 cut(s)	1323, 1856, 2012, 2032, 2288
SmoI	CTYRAG	5 cut(s)	1323, 1856, 2012, 2032, 2288
SseBI	AGGCCT	1 cut(s)	670
SsiI	CCGC	6 cut(s)	110, 456, 493, 1263, 1891, 2473
SspMI	CTAG	6 cut(s)	285, 335, 1374, 1971, 2213, 2250
SstI	GAGCTC	1 cut(s)	2706
StuI	AGGCCT	1 cut(s)	670
StyI	CCWWGG	4 cut(s)	18, 274, 1698, 2346
TaaI	ACNGT	6 cut(s)	599, 906, 1005, 1427, 1686, 2757
TaiI	ACGT	3 cut(s)	2065, 2614, 2776
TaqI	TCGA	7 cut(s)	189, 587, 1090, 1484, 2303, 2415, 2517
TatI	WGTACW	4 cut(s)	627, 1005, 2284, 2391
TauI	GCSGC	1 cut(s)	1894
TfiI	GAWTC	5 cut(s)	808, 850, 859, 2072, 2450
TscAI	CASTG	9 cut(s)	241, 469, 602, 688, 1432, 1712, 2124, 2167, 2762
TseFI	GTSAC	6 cut(s)	119, 224, 368, 713, 1140, 1646
Tsp45I	GTSAC	6 cut(s)	119, 224, 368, 713, 1140, 1646
TspDTI	ATGAA	7 cut(s)	734, 757, 1088, 1746, 1946, 2138, 2738
TspGWI	ACGGA	3 cut(s)	790, 1990, 2641
TspMI	CCCGGG	1 cut(s)	1716
TspRI	CASTG	9 cut(s)	241, 469, 602, 688, 1432, 1712, 2124, 2167, 2762
Tth111I	GACNNNGTC	1 cut(s)	201
Van91I	CCANNNNNTGG	1 cut(s)	2680
Vha464I	CTTAAG	1 cut(s)	1856
VneI	GTGCAC	1 cut(s)	999
VpaK11BI	GGWCC	2 cut(s)	1711, 2541
XagI	CCTNNNNNAGG	1 cut(s)	2639
XapI	RAATTY	5 cut(s)	185, 266, 958, 1993, 2763
XceI	RCATGY	2 cut(s)	1129, 2697
XmaI	CCCGGG	1 cut(s)	1716
XmnI	GAANNNNTTC	1 cut(s)	764
XspI	CTAG	6 cut(s)	285, 335, 1374, 1971, 2213, 2250
ZrmI	AGTACT	1 cut(s)	2286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.