pycom13g20430

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011792
Physical Location & Seq
Reverse (-)
16509886 .. 16512546
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20430.1

Sequence Viewer

Length: 903 bp
ATGACGAGCCCAAGGAATGGAGAGAGGACTGATCAGGTGCAGCAGCAGCAGCAGTCTCCTCCTTACCATGTTCTTCACAAGCTGCCTCCTGGTGACAGCCCTTACGTCAGAGCCAAGCACGTCCAGTTAGTTCAGAAAGATCCGGAAGCGGCTATTGTACTGTTCTGGAAGGCAATAACTGCGGGTGATAGGGTGGATAGTTCCCTCAAAGACATGGCAGTGGTCATGAAGCAACAGGATAGAGCAGAAGAAGCAATTGAAGCAATAAAGTCTTTCAGGGATCGGTGCTCCAGGCAAGCACAGGAATCCCTTGACAATGTACTAATCGACTTGTACAAGAAATGTGGAAGAGTTGAGGAACAAATAGAGCTATTAAAACAGAAGCTTCGGATGATTTTCCAGGGAGAGGTCTTCAATGGCAAGCACACCAAGACAGCGCGCTCTCATGGAAGGAAATTCCAAGTCACAATCAAGCAAGAAACCTCCAGGATACTGGGCAACTTAGGTTGGGCATACATGCAACAAGGGAACCATGCGGCTGCAGAAGTGGTGTATCGTAAAGCCCAAATCATTGACCCGGATGCAAACAAGGCCTGCAACTTATGCCTGTGCCTGATCAAGCAAAGAAGGTATGCGGAGGCGCAATCAGTTGTTGACCTTGTGTTGAAGGGCATGCTTCCGGGATCTGATGAGCCCAAGTCAAAAGTTCGTGCCGAGGAACTGGTGCAGGAGCTAGAGCAATGTCAACGTGTAGTTCTACCTCCCAATTCGTTAAGTTTAAATATGGAAGATGCCTTTCTCGAGGGGCTTGACCACTTGGTGAAGCAGTGGACTCCTCAAAGGTCGAGGAGACTTGCTATATTTGAAGAGATATCGTCGTTTAGAGATCAGTTAGCTTGCTGA

Protein Analysis

301

Amino Acids

34.23

Weight (kDa)

8.75

Isoelectric Point (pI)

54.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_1 PF00515 163 - 194 2.3e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04770
fragaria_vesca FvH4_4g05910 FvH4_4g05910
malus_domestica MD13G1231400.v1.1
prunus_persica Prupe.1G061500_v2.0.a1
pyrus_communis pycom13g20430
rosa_chinensis RchiOBHm_Chr4g0397051
rosa_laevigata RLG00000009453
rosa_multiflora Rmu_sc0007785.1_g000009
rosa_roxburghii Rroxscaffold_5G00342050
rosa_rugosa Rorug03G0345200 Rorug03G0345300 Rorug03G0345400
rosa_samantha Rh4AG076800 Rh4BG072700 Rh4CG081700 Rh4DG070200
rosa_wichuraiana Rw0G020190 Rw4G006180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 17
AccII CGCG 1 cut(s) 439
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 4 cut(s) 149, 182, 536, 635
AclWI GGATC 3 cut(s) 134, 288, 691
AcsI RAATTY 1 cut(s) 455
AdeI CACNNNGTG 1 cut(s) 820
AfaI GTAC 3 cut(s) 159, 321, 335
AfiI CCNNNNNNNGG 2 cut(s) 17, 406
AflIII ACRYGT 1 cut(s) 748
AgsI TTSAA 4 cut(s) 260, 415, 667, 866
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 4 cut(s) 88, 290, 399, 485
AluBI AGCT 5 cut(s) 82, 370, 385, 733, 896
AluI AGCT 5 cut(s) 82, 370, 385, 733, 896
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 60, 844
AlwI GGATC 3 cut(s) 134, 288, 691
Ama87I CYCGRG 1 cut(s) 800
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 1 cut(s) 591
ApeKI GCWGC 6 cut(s) 40, 43, 46, 49, 82, 539
ApoI RAATTY 1 cut(s) 455
AspLEI GCGC 3 cut(s) 439, 441, 643
AsuC2I CCSGG 2 cut(s) 578, 681
AsuHPI GGTGA 3 cut(s) 104, 197, 832
AvaI CYCGRG 1 cut(s) 800
BanII GRGCYC 2 cut(s) 11, 696
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 6 cut(s) 52, 55, 58, 61, 69, 526
BciT130I CCWGG 4 cut(s) 90, 292, 401, 487
BciVI GTATCC 1 cut(s) 483
BclI TGATCA 2 cut(s) 31, 615
BcnI CCSGG 2 cut(s) 578, 681
BcoDI GTCTC 2 cut(s) 60, 844
BfaI CTAG 1 cut(s) 734
BfmI CTRYAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 8 cut(s) 41, 44, 47, 50, 83, 150, 537, 540
BlsI GCNGC 8 cut(s) 42, 45, 48, 51, 84, 151, 538, 541
Bme1390I CCNGG 6 cut(s) 90, 292, 401, 487, 578, 681
BmeT110I CYCGRG 1 cut(s) 800
BmgBI CACGTC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 530
BmrFI CCNGG 6 cut(s) 90, 292, 401, 487, 578, 681
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 2 cut(s) 571, 781
BmuI ACTGGG 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 403
BpmI CTGGAG 2 cut(s) 274, 469
BpuMI CCSGG 2 cut(s) 578, 681
BsaJI CCNNGG 3 cut(s) 11, 400, 714
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 406
Bse1I ACTGG 3 cut(s) 124, 498, 726
Bse3DI GCAATG 1 cut(s) 746
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 4 cut(s) 90, 292, 401, 487
BseDI CCNNGG 3 cut(s) 11, 400, 714
BseGI GGATG 2 cut(s) 396, 586
BseLI CCNNNNNNNGG 2 cut(s) 17, 406
BseMI GCAATG 1 cut(s) 746
BseNI ACTGG 3 cut(s) 124, 498, 726
BsePI GCGCGC 1 cut(s) 437
BseRI GAGGAG 3 cut(s) 48, 825, 862
BseXI GCAGC 6 cut(s) 52, 55, 58, 61, 69, 526
BsgI GTGCAG 2 cut(s) 59, 746
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 1 cut(s) 593
BsiHKAI GWGCWC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 3 cut(s) 143, 578, 680
BslI CCNNNNNNNGG 2 cut(s) 17, 406
BsmAI GTCTC 2 cut(s) 60, 844
BsnI GGCC 1 cut(s) 593
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 3 cut(s) 11, 290, 696
Bsp13I TCCGGA 1 cut(s) 142
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 6 cut(s) 31, 139, 280, 615, 683, 886
BspACI CCGC 4 cut(s) 149, 182, 536, 635
BspANI GGCC 1 cut(s) 593
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 1 cut(s) 439
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 530
BspMAI CTGCAG 1 cut(s) 544
BspPI GGATC 3 cut(s) 134, 288, 691
BsrDI GCAATG 1 cut(s) 746
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 3 cut(s) 124, 498, 726
BssECI CCNNGG 3 cut(s) 11, 400, 714
BssHII GCGCGC 1 cut(s) 437
BssMI GATC 6 cut(s) 31, 139, 280, 615, 683, 886
BssT1I CCWWGG 1 cut(s) 11
Bst2UI CCWGG 4 cut(s) 90, 292, 401, 487
Bst4CI ACNGT 1 cut(s) 162
Bst6I CTCTTC 2 cut(s) 343, 861
BstAPI GCANNNNNTGC 2 cut(s) 179, 603
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 6 cut(s) 297, 422, 439, 595, 674, 898
BstDEI CTNAG 1 cut(s) 502
BstF5I GGATG 2 cut(s) 396, 586
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 3 cut(s) 439, 441, 643
BstKTI GATC 6 cut(s) 34, 142, 283, 618, 686, 889
BstMAI GTCTC 2 cut(s) 60, 844
BstMBI GATC 6 cut(s) 31, 139, 280, 615, 683, 886
BstMWI GCNNNNNNNGC 7 cut(s) 46, 49, 179, 251, 260, 590, 603
BstNI CCWGG 4 cut(s) 90, 292, 401, 487
BstNSI RCATGY 2 cut(s) 520, 676
BstSCI CCNGG 6 cut(s) 88, 290, 399, 485, 576, 679
BstSFI CTRYAG 1 cut(s) 540
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 6 cut(s) 52, 55, 58, 61, 69, 526
BstV2I GAAGAC 1 cut(s) 403
BstX2I RGATCY 2 cut(s) 139, 683
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 2 cut(s) 139, 683
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 1 cut(s) 593
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 2 cut(s) 396, 586
BtsI GCAGTG 2 cut(s) 225, 833
BtsIMutI CAGTG 2 cut(s) 225, 833
Cac8I GCNNGC 6 cut(s) 297, 422, 439, 595, 674, 898
CciI TCATGA 1 cut(s) 225
CfoI GCGC 3 cut(s) 439, 441, 643
Csp6I GTAC 3 cut(s) 158, 320, 334
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 7 cut(s) 68, 214, 226, 446, 517, 533, 673
CviQI GTAC 3 cut(s) 158, 320, 334
DdeI CTNAG 1 cut(s) 502
DpnI GATC 6 cut(s) 33, 141, 282, 617, 685, 888
DpnII GATC 6 cut(s) 31, 139, 280, 615, 683, 886
DraI TTTAAA 1 cut(s) 780
DraIII CACNNNGTG 1 cut(s) 820
Eam1104I CTCTTC 2 cut(s) 343, 861
EarI CTCTTC 2 cut(s) 343, 861
Eco130I CCWWGG 1 cut(s) 11
Eco147I AGGCCT 1 cut(s) 593
Eco24I GRGCYC 2 cut(s) 11, 696
Eco32I GATATC 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 800
EcoRII CCWGG 4 cut(s) 88, 290, 399, 485
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 1 cut(s) 11
EcoT38I GRGCYC 2 cut(s) 11, 696
ErhI CCWWGG 1 cut(s) 11
FaeI CATG 7 cut(s) 71, 217, 229, 449, 520, 536, 676
FatI CATG 7 cut(s) 67, 213, 225, 445, 516, 532, 672
FauI CCCGC 1 cut(s) 175
FbaI TGATCA 2 cut(s) 31, 615
Fnu4HI GCNGC 8 cut(s) 41, 44, 47, 50, 83, 150, 537, 540
FokI GGATG 2 cut(s) 403, 593
FriOI GRGCYC 2 cut(s) 11, 696
Fsp4HI GCNGC 8 cut(s) 41, 44, 47, 50, 83, 150, 537, 540
FspBI CTAG 1 cut(s) 734
GlaI GCGC 3 cut(s) 438, 440, 642
GluI GCNGC 8 cut(s) 41, 44, 47, 50, 83, 150, 537, 540
GsuI CTGGAG 2 cut(s) 274, 469
HaeIII GGCC 1 cut(s) 593
HapII CCGG 3 cut(s) 143, 578, 680
HhaI GCGC 3 cut(s) 439, 441, 643
Hin1II CATG 7 cut(s) 71, 217, 229, 449, 520, 536, 676
Hin6I GCGC 3 cut(s) 437, 439, 641
HinP1I GCGC 3 cut(s) 437, 439, 641
HincII GTYRAC 2 cut(s) 655, 746
HindII GTYRAC 2 cut(s) 655, 746
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 2 cut(s) 305, 832
HpaII CCGG 3 cut(s) 143, 578, 680
HphI GGTGA 3 cut(s) 104, 197, 832
Hpy166II GTNNAC 3 cut(s) 655, 746, 831
Hpy188I TCNGA 4 cut(s) 110, 135, 390, 688
Hpy188III TCNNGA 4 cut(s) 143, 166, 226, 800
Hpy8I GTNNAC 3 cut(s) 655, 746, 831
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 4 cut(s) 163, 444, 621, 661
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 3 cut(s) 105, 120, 748
HpyCH4V TGCA 6 cut(s) 40, 520, 542, 584, 597, 727
HpyF10VI GCNNNNNNNGC 7 cut(s) 46, 49, 179, 251, 260, 590, 603
HpyF3I CTNAG 1 cut(s) 502
HpySE526I ACGT 3 cut(s) 105, 120, 748
Hsp92II CATG 7 cut(s) 71, 217, 229, 449, 520, 536, 676
HspAI GCGC 3 cut(s) 437, 439, 641
Kpn2I TCCGGA 1 cut(s) 142
Ksp22I TGATCA 2 cut(s) 31, 615
Kzo9I GATC 6 cut(s) 31, 139, 280, 615, 683, 886
LmnI GCTCC 2 cut(s) 293, 730
Lsp1109I GCAGC 6 cut(s) 52, 55, 58, 61, 69, 526
LweI GCATC 2 cut(s) 571, 781
MaeI CTAG 1 cut(s) 734
MaeII ACGT 3 cut(s) 105, 120, 748
MaeIII GTNAC 2 cut(s) 92, 463
MalI GATC 6 cut(s) 33, 141, 282, 617, 685, 888
MboI GATC 6 cut(s) 31, 139, 280, 615, 683, 886
MboII GAAGA 6 cut(s) 65, 260, 360, 403, 800, 878
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 2 cut(s) 139, 683
MhlI GDGCHC 3 cut(s) 11, 290, 696
MluCI AATT 3 cut(s) 255, 455, 766
MlyI GAGTC 1 cut(s) 826
MroI TCCGGA 1 cut(s) 142
MseI TTAA 3 cut(s) 374, 773, 779
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 3 cut(s) 143, 578, 680
MspR9I CCNGG 6 cut(s) 90, 292, 401, 487, 578, 681
MunI CAATTG 1 cut(s) 255
MvaI CCWGG 4 cut(s) 90, 292, 401, 487
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 7 cut(s) 46, 49, 179, 251, 260, 590, 603
NciI CCSGG 2 cut(s) 578, 681
NdeII GATC 6 cut(s) 31, 139, 280, 615, 683, 886
NlaIII CATG 7 cut(s) 71, 217, 229, 449, 520, 536, 676
NlaIV GGNNCC 1 cut(s) 530
NmeAIII GCCGAG 1 cut(s) 739
NmuCI GTSAC 2 cut(s) 92, 463
NspI RCATGY 2 cut(s) 520, 676
PaeI GCATGC 1 cut(s) 676
PaeR7I CTCGAG 1 cut(s) 800
PagI TCATGA 1 cut(s) 225
PauI GCGCGC 1 cut(s) 437
PceI AGGCCT 1 cut(s) 593
PfeI GAWTC 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 17
PfoI TCCNGGA 2 cut(s) 485, 679
PkrI GCNGC 8 cut(s) 42, 45, 48, 51, 84, 151, 538, 541
PleI GAGTC 1 cut(s) 826
PpsI GAGTC 1 cut(s) 826
Psp6I CCWGG 4 cut(s) 88, 290, 399, 485
PspGI CCWGG 4 cut(s) 88, 290, 399, 485
PspN4I GGNNCC 1 cut(s) 530
PstI CTGCAG 1 cut(s) 544
PsuI RGATCY 2 cut(s) 139, 683
PteI GCGCGC 1 cut(s) 437
RsaI GTAC 3 cut(s) 159, 321, 335
RsaNI GTAC 3 cut(s) 158, 320, 334
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 3 cut(s) 374, 773, 779
SatI GCNGC 8 cut(s) 41, 44, 47, 50, 83, 150, 537, 540
Sau3AI GATC 6 cut(s) 31, 139, 280, 615, 683, 886
SchI GAGTC 1 cut(s) 826
ScrFI CCNGG 6 cut(s) 90, 292, 401, 487, 578, 681
SduI GDGCHC 3 cut(s) 11, 290, 696
SfaNI GCATC 2 cut(s) 571, 781
SfcI CTRYAG 1 cut(s) 540
Sfr274I CTCGAG 1 cut(s) 800
SlaI CTCGAG 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
SphI GCATGC 1 cut(s) 676
Sse9I AATT 3 cut(s) 255, 455, 766
SseBI AGGCCT 1 cut(s) 593
SsiI CCGC 4 cut(s) 149, 182, 536, 635
SspMI CTAG 1 cut(s) 734
StuI AGGCCT 1 cut(s) 593
StyD4I CCNGG 6 cut(s) 88, 290, 399, 485, 576, 679
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 3 cut(s) 108, 123, 751
TaqI TCGA 3 cut(s) 327, 801, 845
TasI AATT 3 cut(s) 255, 455, 766
TatI WGTACW 3 cut(s) 157, 319, 333
TauI GCSGC 2 cut(s) 152, 539
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 3 cut(s) 374, 773, 779
Tru9I TTAA 3 cut(s) 374, 773, 779
TscAI CASTG 2 cut(s) 225, 833
TseFI GTSAC 2 cut(s) 92, 463
TseI GCWGC 6 cut(s) 40, 43, 46, 49, 82, 539
Tsp45I GTSAC 2 cut(s) 92, 463
TspDTI ATGAA 1 cut(s) 242
TspRI CASTG 2 cut(s) 225, 833
Van91I CCANNNNNTGG 1 cut(s) 17
XapI RAATTY 1 cut(s) 455
XceI RCATGY 2 cut(s) 520, 676
XhoI CTCGAG 1 cut(s) 800
XspI CTAG 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.