Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0005666

Physical Location & Seq

Genomic Coordinates Reverse (-)

1195489 .. 1201302

Gene Structure 5814 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGACTCGGAATGAATTTGGAGTCTGGGAGATCTTTTTGCCAAACAATGCAGATGGCTCACCTTCGATTCCACATGGTTCTCGAGTGAAGATCCGTATGGATACTCCTTCAGGCATCAAAGACTCGATTTCTGCTTGGATCAAGTTTTCTGTGCAGGCTCCAGGTGAAATCCCATATAATGGCATATACTATGATCCACCAGAAGAGGAAAAGTACGTATTTCAACATTCTCAGCCGACGAGACCAAAATCACTTCGCATTTATGAGGCACATGTTGGAATGAGTAGCACGGAGCCCAAAATTAACACATATGCTGAATTCAGAGATGATGTGCTTCCACGCATTAAGAAGCTTGGTTACAATGCTGTTCAGCTCATGGCCATTCAAGAGCACTCCTACTATGCTAGCTTTGGGTACCATGTCACCAATTTTTTTGCACCTAGCAGTCGCTGTGGAACCCCTGACGATCTGAAGTCTCTGATAGATAAAGCTCACGAGCTTGGTCTGCTTGTACTTATGGATATTGTTCACAGCCATGCATCAAATAACACGTTGGATGGGTTGAACATGTTTGATGGAACCGATAGTCATTATTTCCACTCTGGGTCTCGAGGATATCATTGGATGTGGGATTCCCGCTTGTTTAATTATGGAAGCTGGGAGGTTTTGAGGTATCTTCTTTCCAATGCAAGGTGGTGGCTGGAAGAATACAAGTTCGATGGGTTCAGATTTGATGGTGTGACTTCAATGATGTACACTCACCATGGATTGAAGGTAGCATTTACTGGAAATTACAGTGAGTACTTTGGATTAGCAACAGATGTAGATGCTGTGACTTACCTGATGCTGGTTAACGATCTGATTCACGGGCTTTACCCTGAAGCTGTTACCGTTGGTGAAGATGTTAGTGGAATGCCAACTTTCTGTGTTGCTGTTACCGATGGTGGTGTTGGATTTGATTATCGCCTGCAAATGGCTATTGCTGATAAATGGATTGAGCTCCTTCAGAAAAGGGATGAAGAGTGGAAAATGGGTGATATTGTTTTCACGCTCACCAACAGAAGGTGGCGGGAGAACTGTGTTGCTTATGCCGAAAGTCATGACCAAGCTCTTGTTGGTGACAAAACAATTGCATTTTGGCTGATGGACAAGGATATGTATGATTTCATGGCTTTGGACAGACCATCAACTCCCCGTATAGATCGTGGAATAGCATTACATAAAATGATTAGGCTTATTACTATGGGATTAGGCGGAGAAGGATATCTGAATTTCATGGGAAATGAATTTGGCCATCCTGAGTGGATTGATTTTCCAAGAGGTGCTCAACATCTTCCAAGCGGTAAAATAGTTCCTGGGAACAATAACAGTTTTGACAAATGCCGACGTAGATTTGATCTTGGGGATGCTGACTATCTAAGATATCACGGGATGCAAGAATTTGATCGAGCAATGCAACATCTCGAACAAACCTATGGTTTCATGACTTCCGAGCACCAATATATATCACGGAAGGATGAAGGAGACAAAATTATTGTCTTTGAAAGGGGAGACCTGGTTTTTGTCTTCAATTTTCATTGGAGTAAAAGCTATTCAGACTACCGAATTGGCTGCTTAAAGCCAGGAAAGTACAAGATTGTCTTGGATTCAGATGAAAAGTTATTTGGGGGCTTCGATAGGCTTGATCACAGTGCTGAGTACTTCACGACTGATGGGTGGTTTGATGACCGGCCTCATTCTTTCCTCCTCTATGCACCTTGTAGAACAGCTGTGGTCTACGCTCTTGTAGAGGGACCTAAAAAAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001678 GO:0003674 GO:0003824 GO:0003844 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009501 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009987 GO:0010021 GO:0010033 GO:0016740 GO:0016757 GO:0016758 GO:0019725 GO:0033500 GO:0034284 GO:0034285 GO:0042221 GO:0042592 GO:0042593 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071326 GO:0071329 GO:0071331 GO:0071332 GO:0071333 GO:0071478 GO:0071482 GO:0071704 GO:0104004 GO:1901135 GO:1901137 GO:1901576 GO:1901700 GO:1901701 GO:2000896

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

603

Amino Acids

69.34

Weight (kDa)

5.67

Isoelectric Point (pI)

31.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	112 - 370	7.6e-12	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	501 - 595	6.5e-24	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36390 AT5G03650
fragaria_vesca	FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120 FvH4_6g18120
malus_domestica	MD12G1020600.v1.1 MD14G1017700.v1.1
prunus_persica	Prupe.7G115400_v2.0.a1 Prupe.7G115400_v2.0.a1
pyrus_communis	pycom12g01930 pycom14g01610
rosa_chinensis	RchiOBHm_Chr3g0472421
rosa_laevigata	RLG00000024081
rosa_multiflora	Rmu_co8370739.1_g000001 Rmu_sc0001096.1_g000015
rosa_roxburghii	Rroxscaffold_6G00408930
rosa_rugosa	Rorug03G0124700 Rorug03G0124700 Rorug03G0124800 Rorug03G0124900
rosa_samantha	Rh3AG177100 Rh3BG203100 Rh3CG193400 Rh3CG200600 Rh3DG172000
rosa_wichuraiana	Rw3G016440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	414
AccB1I	GGYRCC	1 cut(s)	414
AccB7I	CCANNNNNTGG	1 cut(s)	179
AccI	GTMKAC	1 cut(s)	1776
AciI	CCGC	4 cut(s)	637, 1069, 1254, 1341
AclWI	GGATC	3 cut(s)	85, 146, 188
AcoI	YGGCCR	2 cut(s)	378, 1291
AcsI	RAATTY	5 cut(s)	14, 317, 1270, 1286, 1439
AcuI	CTGAAG	4 cut(s)	93, 491, 900, 989
AfaI	GTAC	7 cut(s)	215, 416, 513, 755, 803, 1631, 1700
AfiI	CCNNNNNNNGG	6 cut(s)	179, 690, 847, 973, 1062, 1802
AflIII	ACRYGT	3 cut(s)	271, 549, 567
AgsI	TTSAA	7 cut(s)	224, 386, 565, 747, 772, 1544, 1570
AjnI	CCWGG	4 cut(s)	160, 1354, 1554, 1621
AjuI	GAANNNNNNNTTGG	2 cut(s)	1647, 1679
Alw21I	GWGCWC	4 cut(s)	393, 1002, 1327, 1497
Alw26I	GTCTC	5 cut(s)	235, 480, 612, 1518, 1545
AlwI	GGATC	3 cut(s)	85, 146, 188
AlwNI	CAGNNNCTG	1 cut(s)	450
Ama87I	CYCGRG	2 cut(s)	81, 609
AoxI	GGCC	3 cut(s)	378, 1291, 1730
ApeKI	GCWGC	1 cut(s)	1611
ApoI	RAATTY	5 cut(s)	14, 317, 1270, 1286, 1439
ArsI	GACNNNNNNTTYG	4 cut(s)	1521, 1544, 1553, 1576
Asp718I	GGTACC	1 cut(s)	414
AspS9I	GGNCC	1 cut(s)	1793
AsuHPI	GGTGA	8 cut(s)	51, 176, 415, 752, 908, 1045, 1046, 1130
AsuNHI	GCTAGC	1 cut(s)	404
AvaI	CYCGRG	2 cut(s)	81, 609
AvaII	GGWCC	1 cut(s)	1793
BalI	TGGCCA	2 cut(s)	380, 1293
BanI	GGYRCC	1 cut(s)	414
BanII	GRGCYC	2 cut(s)	297, 1002
BarI	GAAGNNNNNNTAC	2 cut(s)	341, 373
BauI	CACGAG	1 cut(s)	494
BbsI	GAAGAC	1 cut(s)	1558
Bbv12I	GWGCWC	4 cut(s)	393, 1002, 1327, 1497
BbvI	GCAGC	1 cut(s)	1598
BcgI	CGANNNNNNTGC	2 cut(s)	1593, 1627
BciT130I	CCWGG	4 cut(s)	162, 1356, 1556, 1623
BciVI	GTATCC	1 cut(s)	94
BclI	TGATCA	1 cut(s)	1684
BcoDI	GTCTC	5 cut(s)	235, 480, 612, 1518, 1545
BfaI	CTAG	2 cut(s)	405, 441
BfuI	GTATCC	1 cut(s)	94
BglII	AGATCT	1 cut(s)	30
BisI	GCNGC	1 cut(s)	1612
BlsI	GCNGC	1 cut(s)	1613
BmcAI	AGTACT	2 cut(s)	803, 1700
Bme1390I	CCNGG	4 cut(s)	162, 1356, 1556, 1623
Bme18I	GGWCC	1 cut(s)	1793
BmeT110I	CYCGRG	2 cut(s)	81, 609
BmgT120I	GGNCC	1 cut(s)	1793
BmiI	GGNNCC	6 cut(s)	159, 294, 416, 457, 580, 1794
BmrFI	CCNGG	4 cut(s)	162, 1356, 1556, 1623
BmsI	GCATC	6 cut(s)	123, 548, 817, 834, 1396, 1422
BmtI	GCTAGC	1 cut(s)	408
BpiI	GAAGAC	1 cut(s)	1558
BpmI	CTGGAG	1 cut(s)	144
BsaAI	YACGTR	1 cut(s)	217
BsaI	GGTCTC	3 cut(s)	235, 612, 1545
BsaJI	CCNNGG	2 cut(s)	763, 1355
BsaXI	ACNNNNNCTCC	2 cut(s)	1542, 1572
Bsc4I	CCNNNNNNNGG	6 cut(s)	179, 690, 847, 973, 1062, 1802
Bse118I	RCCGGY	1 cut(s)	1728
Bse1I	ACTGG	1 cut(s)	790
Bse3DI	GCAATG	1 cut(s)	1458
BseBI	CCWGG	4 cut(s)	162, 1356, 1556, 1623
BseDI	CCNNGG	2 cut(s)	763, 1355
BseGI	GGATG	7 cut(s)	562, 630, 1021, 1294, 1411, 1437, 1522
BseLI	CCNNNNNNNGG	6 cut(s)	179, 690, 847, 973, 1062, 1802
BseMI	GCAATG	1 cut(s)	1458
BseMII	CTCAG	3 cut(s)	245, 1290, 1686
BseNI	ACTGG	1 cut(s)	790
BseRI	GAGGAG	1 cut(s)	1736
BseXI	GCAGC	1 cut(s)	1598
BseYI	CCCAGC	1 cut(s)	657
BsgI	GTGCAG	1 cut(s)	173
BshFI	GGCC	3 cut(s)	380, 1293, 1732
BshNI	GGYRCC	1 cut(s)	414
BsiHKAI	GWGCWC	4 cut(s)	393, 1002, 1327, 1497
BsiHKCI	CYCGRG	2 cut(s)	81, 609
BsiSI	CCGG	1 cut(s)	1729
BslI	CCNNNNNNNGG	6 cut(s)	179, 690, 847, 973, 1062, 1802
BsmAI	GTCTC	5 cut(s)	235, 480, 612, 1518, 1545
BsmI	GAATGC	1 cut(s)	918
BsnI	GGCC	3 cut(s)	380, 1293, 1732
Bso31I	GGTCTC	3 cut(s)	235, 612, 1545
BsoBI	CYCGRG	2 cut(s)	81, 609
Bsp1286I	GDGCHC	5 cut(s)	297, 393, 1002, 1327, 1497
Bsp1407I	TGTACA	1 cut(s)	753
Bsp19I	CCATGG	1 cut(s)	763
BspACI	CCGC	4 cut(s)	637, 1069, 1254, 1341
BspANI	GGCC	3 cut(s)	380, 1293, 1732
BspCNI	CTCAG	3 cut(s)	244, 1291, 1687
BspHI	TCATGA	2 cut(s)	1099, 1482
BspLI	GGNNCC	6 cut(s)	159, 294, 416, 457, 580, 1794
BspOI	GCTAGC	1 cut(s)	408
BspPI	GGATC	3 cut(s)	85, 146, 188
BspT107I	GGYRCC	1 cut(s)	414
BspTNI	GGTCTC	3 cut(s)	235, 612, 1545
BsrDI	GCAATG	1 cut(s)	1458
BsrFI	RCCGGY	1 cut(s)	1728
BsrGI	TGTACA	1 cut(s)	753
BsrI	ACTGG	1 cut(s)	790
BssAI	RCCGGY	1 cut(s)	1728
BssECI	CCNNGG	2 cut(s)	763, 1355
BssSI	CACGAG	1 cut(s)	494
BssT1I	CCWWGG	1 cut(s)	763
Bst2BI	CACGAG	1 cut(s)	494
Bst2UI	CCWGG	4 cut(s)	162, 1356, 1556, 1623
Bst4CI	ACNGT	5 cut(s)	797, 892, 1079, 1370, 1691
Bst6I	CTCTTC	2 cut(s)	198, 1014
BstAUI	TGTACA	1 cut(s)	753
BstBAI	YACGTR	1 cut(s)	217
BstC8I	GCNNGC	3 cut(s)	156, 406, 968
BstDEI	CTNAG	4 cut(s)	231, 1299, 1418, 1695
BstDSI	CCRYGG	1 cut(s)	763
BstENI	CCTNNNNNAGG	1 cut(s)	1800
BstF5I	GGATG	7 cut(s)	562, 630, 1021, 1294, 1411, 1437, 1522
BstMAI	GTCTC	5 cut(s)	235, 480, 612, 1518, 1545
BstMWI	GCNNNNNNNGC	1 cut(s)	505
BstNI	CCWGG	4 cut(s)	162, 1356, 1556, 1623
BstNSI	RCATGY	2 cut(s)	275, 571
BstSCI	CCNGG	4 cut(s)	160, 1354, 1554, 1621
BstSNI	TACGTA	1 cut(s)	217
BstV1I	GCAGC	1 cut(s)	1598
BstV2I	GAAGAC	1 cut(s)	1558
BstX2I	RGATCY	2 cut(s)	30, 90
BstYI	RGATCY	2 cut(s)	30, 90
BsuI	GTATCC	1 cut(s)	94
BsuRI	GGCC	3 cut(s)	380, 1293, 1732
BtgI	CCRYGG	1 cut(s)	763
BtsCI	GGATG	7 cut(s)	562, 630, 1021, 1294, 1411, 1437, 1522
BtsIMutI	CAGTG	2 cut(s)	802, 1696
Cac8I	GCNNGC	3 cut(s)	156, 406, 968
CaiI	CAGNNNCTG	1 cut(s)	450
CciI	TCATGA	2 cut(s)	1099, 1482
Cfr10I	RCCGGY	1 cut(s)	1728
Cfr13I	GGNCC	1 cut(s)	1793
CsiI	ACCWGGT	1 cut(s)	1554
Csp6I	GTAC	7 cut(s)	214, 415, 512, 754, 802, 1630, 1699
CviQI	GTAC	7 cut(s)	214, 415, 512, 754, 802, 1630, 1699
DdeI	CTNAG	4 cut(s)	231, 1299, 1418, 1695
EaeI	YGGCCR	2 cut(s)	378, 1291
Eam1104I	CTCTTC	2 cut(s)	198, 1014
EarI	CTCTTC	2 cut(s)	198, 1014
EciI	GGCGGA	1 cut(s)	1269
Ecl136II	GAGCTC	1 cut(s)	1000
Eco105I	TACGTA	1 cut(s)	217
Eco130I	CCWWGG	1 cut(s)	763
Eco24I	GRGCYC	2 cut(s)	297, 1002
Eco31I	GGTCTC	3 cut(s)	235, 612, 1545
Eco32I	GATATC	3 cut(s)	617, 1265, 1424
Eco47I	GGWCC	1 cut(s)	1793
Eco53kI	GAGCTC	1 cut(s)	1000
Eco57I	CTGAAG	4 cut(s)	93, 491, 900, 989
Eco88I	CYCGRG	2 cut(s)	81, 609
EcoICRI	GAGCTC	1 cut(s)	1000
EcoNI	CCTNNNNNAGG	1 cut(s)	1800
EcoO109I	RGGNCCY	1 cut(s)	1793
EcoRI	GAATTC	1 cut(s)	317
EcoRII	CCWGG	4 cut(s)	160, 1354, 1554, 1621
EcoRV	GATATC	3 cut(s)	617, 1265, 1424
EcoT14I	CCWWGG	1 cut(s)	763
EcoT22I	ATGCAT	1 cut(s)	541
EcoT38I	GRGCYC	2 cut(s)	297, 1002
ErhI	CCWWGG	1 cut(s)	763
FalI	AAGNNNNNCTT	2 cut(s)	1625, 1657
FauI	CCCGC	2 cut(s)	644, 1062
FauNDI	CATATG	1 cut(s)	310
FbaI	TGATCA	1 cut(s)	1684
FblI	GTMKAC	1 cut(s)	1776
Fnu4HI	GCNGC	1 cut(s)	1612
FokI	GGATG	7 cut(s)	569, 637, 1028, 1281, 1418, 1444, 1529
FriOI	GRGCYC	2 cut(s)	297, 1002
Fsp4HI	GCNGC	1 cut(s)	1612
FspBI	CTAG	2 cut(s)	405, 441
GluI	GCNGC	1 cut(s)	1612
GsaI	CCCAGC	1 cut(s)	661
GsuI	CTGGAG	1 cut(s)	144
HaeIII	GGCC	3 cut(s)	380, 1293, 1732
HapII	CCGG	1 cut(s)	1729
HincII	GTYRAC	1 cut(s)	853
HindII	GTYRAC	1 cut(s)	853
HindIII	AAGCTT	1 cut(s)	350
HinfI	GANTC	7 cut(s)	4, 21, 67, 122, 632, 862, 1646
HpaI	GTTAAC	1 cut(s)	853
HpaII	CCGG	1 cut(s)	1729
HphI	GGTGA	8 cut(s)	51, 176, 415, 752, 908, 1045, 1046, 1130
Hpy166II	GTNNAC	4 cut(s)	529, 756, 853, 1777
Hpy188III	TCNNGA	9 cut(s)	81, 386, 494, 609, 1100, 1298, 1463, 1483, 1705
Hpy8I	GTNNAC	4 cut(s)	529, 756, 853, 1777
Hpy99I	CGWCG	2 cut(s)	241, 1389
HpyAV	CCTTC	8 cut(s)	72, 117, 766, 1013, 1056, 1253, 1507, 1514
HpyCH4III	ACNGT	5 cut(s)	797, 892, 1079, 1370, 1691
HpyCH4IV	ACGT	3 cut(s)	216, 551, 1387
HpyF10VI	GCNNNNNNNGC	1 cut(s)	505
HpyF3I	CTNAG	4 cut(s)	231, 1299, 1418, 1695
HpySE526I	ACGT	3 cut(s)	216, 551, 1387
KpnI	GGTACC	1 cut(s)	418
Ksp22I	TGATCA	1 cut(s)	1684
KspAI	GTTAAC	1 cut(s)	853
LmnI	GCTCC	3 cut(s)	163, 292, 1005
Lsp1109I	GCAGC	1 cut(s)	1598
LweI	GCATC	6 cut(s)	123, 548, 817, 834, 1396, 1422
MabI	ACCWGGT	1 cut(s)	1554
MaeI	CTAG	2 cut(s)	405, 441
MaeII	ACGT	3 cut(s)	216, 551, 1387
MaeIII	GTNAC	7 cut(s)	356, 421, 739, 832, 886, 934, 1118
MboII	GAAGA	8 cut(s)	100, 215, 668, 716, 911, 1031, 1325, 1558
MfeI	CAATTG	1 cut(s)	1128
MflI	RGATCY	2 cut(s)	30, 90
MhlI	GDGCHC	5 cut(s)	297, 393, 1002, 1327, 1497
MlsI	TGGCCA	2 cut(s)	380, 1293
MluNI	TGGCCA	2 cut(s)	380, 1293
MlyI	GAGTC	2 cut(s)	30, 116
MmeI	TCCRAC	3 cut(s)	256, 534, 931
Mox20I	TGGCCA	2 cut(s)	380, 1293
Mph1103I	ATGCAT	1 cut(s)	541
MscI	TGGCCA	2 cut(s)	380, 1293
MseI	TTAA	5 cut(s)	303, 345, 645, 852, 1616
MslI	CAYNNNNRTG	1 cut(s)	534
Msp20I	TGGCCA	2 cut(s)	380, 1293
MspA1I	CMGCKG	1 cut(s)	1769
MspI	CCGG	1 cut(s)	1729
MspR9I	CCNGG	4 cut(s)	162, 1356, 1556, 1623
MunI	CAATTG	1 cut(s)	1128
Mva1269I	GAATGC	1 cut(s)	918
MvaI	CCWGG	4 cut(s)	162, 1356, 1556, 1623
MwoI	GCNNNNNNNGC	1 cut(s)	505
NcoI	CCATGG	1 cut(s)	763
NdeI	CATATG	1 cut(s)	310
NheI	GCTAGC	1 cut(s)	404
NlaIV	GGNNCC	6 cut(s)	159, 294, 416, 457, 580, 1794
NmuCI	GTSAC	4 cut(s)	421, 739, 832, 1118
NsiI	ATGCAT	1 cut(s)	541
NspI	RCATGY	2 cut(s)	275, 571
PaeR7I	CTCGAG	2 cut(s)	81, 609
PagI	TCATGA	2 cut(s)	1099, 1482
PciI	ACATGT	2 cut(s)	271, 567
PctI	GAATGC	1 cut(s)	918
PfeI	GAWTC	4 cut(s)	67, 632, 862, 1646
PflMI	CCANNNNNTGG	1 cut(s)	179
PkrI	GCNGC	1 cut(s)	1613
PleI	GAGTC	2 cut(s)	29, 116
PpsI	GAGTC	2 cut(s)	29, 116
Ppu21I	YACGTR	1 cut(s)	217
PpuMI	RGGWCCY	1 cut(s)	1793
PscI	ACATGT	2 cut(s)	271, 567
Psp124BI	GAGCTC	1 cut(s)	1002
Psp5II	RGGWCCY	1 cut(s)	1793
Psp6I	CCWGG	4 cut(s)	160, 1354, 1554, 1621
PspFI	CCCAGC	1 cut(s)	657
PspGI	CCWGG	4 cut(s)	160, 1354, 1554, 1621
PspN4I	GGNNCC	6 cut(s)	159, 294, 416, 457, 580, 1794
PspPI	GGNCC	1 cut(s)	1793
PspPPI	RGGWCCY	1 cut(s)	1793
PstNI	CAGNNNCTG	1 cut(s)	450
PsuI	RGATCY	2 cut(s)	30, 90
PvuII	CAGCTG	1 cut(s)	1769
RsaI	GTAC	7 cut(s)	215, 416, 513, 755, 803, 1631, 1700
RsaNI	GTAC	7 cut(s)	214, 415, 512, 754, 802, 1630, 1699
RseI	CAYNNNNRTG	1 cut(s)	534
SacI	GAGCTC	1 cut(s)	1002
SaqAI	TTAA	5 cut(s)	303, 345, 645, 852, 1616
SatI	GCNGC	1 cut(s)	1612
Sau96I	GGNCC	1 cut(s)	1793
ScaI	AGTACT	2 cut(s)	803, 1700
SchI	GAGTC	2 cut(s)	30, 116
ScrFI	CCNGG	4 cut(s)	162, 1356, 1556, 1623
SduI	GDGCHC	5 cut(s)	297, 393, 1002, 1327, 1497
SexAI	ACCWGGT	1 cut(s)	1554
SfaNI	GCATC	6 cut(s)	123, 548, 817, 834, 1396, 1422
Sfr274I	CTCGAG	2 cut(s)	81, 609
SinI	GGWCC	1 cut(s)	1793
SlaI	CTCGAG	2 cut(s)	81, 609
SmiMI	CAYNNNNRTG	1 cut(s)	534
SmlI	CTYRAG	2 cut(s)	81, 609
SmoI	CTYRAG	2 cut(s)	81, 609
SnaBI	TACGTA	1 cut(s)	217
SsiI	CCGC	4 cut(s)	637, 1069, 1254, 1341
SspMI	CTAG	2 cut(s)	405, 441
SstI	GAGCTC	1 cut(s)	1002
StyD4I	CCNGG	4 cut(s)	160, 1354, 1554, 1621
StyI	CCWWGG	1 cut(s)	763
TaaI	ACNGT	5 cut(s)	797, 892, 1079, 1370, 1691
TaiI	ACGT	3 cut(s)	219, 554, 1390
TaqI	TCGA	8 cut(s)	65, 82, 125, 610, 717, 1447, 1464, 1674
TatI	WGTACW	5 cut(s)	511, 753, 801, 1629, 1698
TfiI	GAWTC	4 cut(s)	67, 632, 862, 1646
Tru1I	TTAA	5 cut(s)	303, 345, 645, 852, 1616
Tru9I	TTAA	5 cut(s)	303, 345, 645, 852, 1616
TscAI	CASTG	2 cut(s)	802, 1696
TseFI	GTSAC	4 cut(s)	421, 739, 832, 1118
TseI	GCWGC	1 cut(s)	1611
Tsp45I	GTSAC	4 cut(s)	421, 739, 832, 1118
TspDTI	ATGAA	9 cut(s)	27, 1032, 1156, 1264, 1299, 1471, 1533, 1565, 1668
TspGWI	ACGGA	3 cut(s)	83, 305, 1525
TspRI	CASTG	2 cut(s)	802, 1696
Van91I	CCANNNNNTGG	1 cut(s)	179
VpaK11BI	GGWCC	1 cut(s)	1793
XagI	CCTNNNNNAGG	1 cut(s)	1800
XapI	RAATTY	5 cut(s)	14, 317, 1270, 1286, 1439
XceI	RCATGY	2 cut(s)	275, 571
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1112
XhoI	CTCGAG	2 cut(s)	81, 609
XmiI	GTMKAC	1 cut(s)	1776
XspI	CTAG	2 cut(s)	405, 441
ZrmI	AGTACT	2 cut(s)	803, 1700
Zsp2I	ATGCAT	1 cut(s)	541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.