pycom14g03850

Sterile alpha motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006591
Physical Location & Seq
Forward (+)
3314743 .. 3315234
492 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGAGGATTAAATCTTTTGCTCAGAAAAGGGTTGTTCAAGTTACTAGAAAATCTCCTCCTGCTCACTACGTTTCAACATTTGGGAAGTCAATCAAGCGCGATGTAAGAATGGAATTACGAAATCCAGATCAGATTGCTACTTCACAGTCTCGTAGAACACCGGGGGTATTTAAAACCTCAAAAGCCATCATTCAGCCAAGAAAATTACGACTTGGCCCGAGTATTTTCCTTGATCAGGGAATGCTGCTGAATCAAAAGCTGAGAGCTCTGAATCTTGAGAGGAAGCTTCAGAAAGCTGGCGGGTTAAGCAGGTGGTTAGCATCACTAGGACTGGGGCAGTTTGTTAGGATTTTCCAGAGGAAAGGTCTGAGTAAGTTCCAGTTGGTGAATCTGACCATGAAGAAGCTCAAAGACATGGGTGCCAATGCAGTAGGGCCCCGGAGGAAACTGATGCACGCAATCGACTGCTTTTGCCAGCCGTGCTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAM_2

Protein Analysis

164

Amino Acids

18.61

Weight (kDa)

11.67

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_2 PF07647 101 - 156 7.8e-15 SAM domain (Sterile alpha motif)
SAM_1 PF00536 102 - 156 2.6e-10 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 300
Acc36I ACCTGC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 419
AccII CGCG 1 cut(s) 99
AciI CCGC 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 2 cut(s) 38, 75
AluBI AGCT 5 cut(s) 259, 266, 286, 296, 406
AluI AGCT 5 cut(s) 259, 266, 286, 296, 406
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 1 cut(s) 153
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 2 cut(s) 214, 434
ApaI GGGCCC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 244
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 3 cut(s) 215, 434, 435
AsuC2I CCSGG 2 cut(s) 162, 439
AsuHPI GGTGA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 217
BaeGI GKGCMC 1 cut(s) 438
BanI GGYRCC 1 cut(s) 419
BanII GRGCYC 2 cut(s) 268, 438
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 1 cut(s) 231
BccI CCATC 1 cut(s) 194
BceAI ACGGC 1 cut(s) 463
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 2 cut(s) 162, 439
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 2 cut(s) 45, 326
BfuAI ACCTGC 1 cut(s) 300
BisI GCNGC 1 cut(s) 245
BlsI GCNGC 1 cut(s) 246
Bme1390I CCNGG 2 cut(s) 162, 439
BmeT110I CYCGRG 1 cut(s) 217
BmgT120I GGNCC 3 cut(s) 215, 434, 435
BmiI GGNNCC 3 cut(s) 421, 436, 437
BmrFI CCNGG 2 cut(s) 162, 439
BmrI ACTGGG 1 cut(s) 341
BmsI GCATC 2 cut(s) 329, 441
BmuI ACTGGG 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 2 cut(s) 162, 439
BsaJI CCNNGG 2 cut(s) 161, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 336, 379
BseDI CCNNGG 2 cut(s) 161, 437
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMII CTCAG 3 cut(s) 35, 251, 359
BseNI ACTGG 2 cut(s) 336, 379
BseRI GAGGAG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 216, 436
BshNI GGYRCC 1 cut(s) 419
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 2 cut(s) 161, 439
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 1 cut(s) 153
BsmI GAATGC 1 cut(s) 246
BsnI GGCC 2 cut(s) 216, 436
BsoBI CYCGRG 1 cut(s) 217
Bsp120I GGGCCC 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 268, 438
Bsp143I GATC 2 cut(s) 127, 232
BspACI CCGC 1 cut(s) 300
BspANI GGCC 2 cut(s) 216, 436
BspCNI CTCAG 3 cut(s) 34, 252, 360
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 3 cut(s) 421, 436, 437
BspMI ACCTGC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 419
BsrI ACTGG 2 cut(s) 336, 379
BssECI CCNNGG 2 cut(s) 161, 437
BssMI GATC 2 cut(s) 127, 232
Bst4CI ACNGT 1 cut(s) 147
BstC8I GCNNGC 3 cut(s) 298, 456, 476
BstDEI CTNAG 3 cut(s) 21, 260, 368
BstENI CCTNNNNNAGG 1 cut(s) 233
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 2 cut(s) 130, 235
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 2 cut(s) 127, 232
BstMWI GCNNNNNNNGC 2 cut(s) 306, 480
BstSCI CCNGG 2 cut(s) 160, 437
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 1 cut(s) 231
BsuRI GGCC 2 cut(s) 216, 436
BtgZI GCGATG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 300
Cac8I GCNNGC 3 cut(s) 298, 456, 476
CfoI GCGC 1 cut(s) 99
Cfr13I GGNCC 3 cut(s) 215, 434, 435
CviAII CATG 2 cut(s) 397, 415
DdeI CTNAG 3 cut(s) 21, 260, 368
DpnI GATC 2 cut(s) 129, 234
DpnII GATC 2 cut(s) 127, 232
DraI TTTAAA 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 266
Eco24I GRGCYC 2 cut(s) 268, 438
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 266
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 434, 435
EcoT38I GRGCYC 2 cut(s) 268, 438
FaeI CATG 2 cut(s) 400, 418
FaiI YATR 2 cut(s) 398, 416
FatI CATG 2 cut(s) 396, 414
FauI CCCGC 1 cut(s) 293
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 245
FriOI GRGCYC 2 cut(s) 268, 438
Fsp4HI GCNGC 1 cut(s) 245
FspBI CTAG 2 cut(s) 45, 326
GlaI GCGC 1 cut(s) 98
GluI GCNGC 1 cut(s) 245
HaeIII GGCC 2 cut(s) 216, 436
HapII CCGG 2 cut(s) 161, 439
HhaI GCGC 1 cut(s) 99
Hin1II CATG 2 cut(s) 400, 418
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 250, 271, 388
HpaII CCGG 2 cut(s) 161, 439
HphI GGTGA 1 cut(s) 397
Hpy188I TCNGA 6 cut(s) 24, 132, 270, 291, 369, 393
Hpy188III TCNNGA 3 cut(s) 125, 275, 355
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 428, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 480
HpyF3I CTNAG 3 cut(s) 21, 260, 368
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 2 cut(s) 400, 418
HspAI GCGC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 2 cut(s) 127, 232
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 2 cut(s) 329, 441
MaeI CTAG 2 cut(s) 45, 326
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 40
MalI GATC 2 cut(s) 129, 234
MboI GATC 2 cut(s) 127, 232
MboII GAAGA 1 cut(s) 412
MhlI GDGCHC 2 cut(s) 268, 438
MluCI AATT 2 cut(s) 113, 203
MnlI CCTC 5 cut(s) 66, 187, 273, 351, 435
MseI TTAA 4 cut(s) 9, 171, 305, 490
MspI CCGG 2 cut(s) 161, 439
MspR9I CCNGG 2 cut(s) 162, 439
Mva1269I GAATGC 1 cut(s) 246
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 306, 480
NciI CCSGG 2 cut(s) 162, 439
NdeII GATC 2 cut(s) 127, 232
NlaIII CATG 2 cut(s) 400, 418
NlaIV GGNNCC 3 cut(s) 421, 436, 437
PaqCI CACCTGC 1 cut(s) 300
PctI GAATGC 1 cut(s) 246
PfeI GAWTC 3 cut(s) 250, 271, 388
PkrI GCNGC 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 268
PspN4I GGNNCC 3 cut(s) 421, 436, 437
PspOMI GGGCCC 1 cut(s) 434
PspPI GGNCC 3 cut(s) 215, 434, 435
SacI GAGCTC 1 cut(s) 268
SaqAI TTAA 4 cut(s) 9, 171, 305, 490
SatI GCNGC 1 cut(s) 245
Sau3AI GATC 2 cut(s) 127, 232
Sau96I GGNCC 3 cut(s) 215, 434, 435
ScrFI CCNGG 2 cut(s) 162, 439
SduI GDGCHC 2 cut(s) 268, 438
SetI ASST 9 cut(s) 72, 179, 261, 268, 288, 298, 314, 367, 408
SfaNI GCATC 2 cut(s) 329, 441
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 2 cut(s) 113, 203
SsiI CCGC 1 cut(s) 300
SspMI CTAG 2 cut(s) 45, 326
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 160, 437
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 462
TasI AATT 2 cut(s) 113, 203
TfiI GAWTC 3 cut(s) 250, 271, 388
Tru1I TTAA 4 cut(s) 9, 171, 305, 490
Tru9I TTAA 4 cut(s) 9, 171, 305, 490
TseI GCWGC 1 cut(s) 244
TspDTI ATGAA 1 cut(s) 413
XagI CCTNNNNNAGG 1 cut(s) 233
XspI CTAG 2 cut(s) 45, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.