pycom14g10300

Sin3 binding region of histone deacetylase complex subunit SAP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010563
Physical Location & Seq
Reverse (-)
12811519 .. 12813782
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGCTGGAGGCAGAGCTCTGTGGTTCTCGGATTCTATTGCCTTTTCGCGAAGAAAGCGGGGATGAGGAACTTTCAGTTCTTCCCAGGCACACAAAGGTTATTGTCACTGGAAACAACAGAACAAAGTCTATTTTGGTGGGTCTTCAAGGCGTGGTCAAGAAAGCTGTTGGGCTTGGGGGTTGGCACTGGCTGGTTCTGAAAAATGGGGTTGAAGTTAAGCTGCAAAGGAATGCATTGAGTGTGCTCGAACATCCTACAGGAAATGAGGAAGATTCCAATCTTGAGTACTCTAGCAGCAGCTCTGACCACGGTGATAAGGAAAATGATTTCTCTAGATGTTTTGAGTTCCATAAGCATAGTAAACCAAGAGTTCGATTTTCAAGGCCATATGTTCCATCCTCAGCAACGAAGTCAATGAATCGTGGCAGTTATAGAGAAGTTCAATACATCCAAGCACCCCAGTCGAGAGTAAACTTGGGAAAACTAGGAACAAGCTCATTGAGGAGGTATTGCGGACACTTCAATCTTTTAAGCGGGAATTCTAATCCAACAAGGGAACAGTTGATCAATGCTGCACAACGACATTTTACCTTGCAGCAGCTGCCGTTGGATGAGGTATCTGTGGTCACTGAATTCGTCGATGCTGCCAGGAGTAGGAAGTGGAAACAAGCAGAGCGACAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAP30_Sin3_bdg

Protein Analysis

229

Amino Acids

25.78

Weight (kDa)

9.42

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP30_Sin3_bdg PF13867 162 - 217 1.8e-15 Sin3 binding region of histone deacetylase complex subunit SAP30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 48
AciI CCGC 3 cut(s) 57, 513, 534
AcsI RAATTY 2 cut(s) 538, 632
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 654
AgsI TTSAA 5 cut(s) 146, 212, 381, 443, 523
AjnI CCWGG 2 cut(s) 83, 647
AluBI AGCT 6 cut(s) 16, 164, 220, 300, 495, 601
AluI AGCT 6 cut(s) 16, 164, 220, 300, 495, 601
Alw21I GWGCWC 2 cut(s) 18, 246
AlwNI CAGNNNCTG 1 cut(s) 601
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 8 cut(s) 220, 294, 297, 572, 595, 598, 601, 644
ApoI RAATTY 2 cut(s) 538, 632
AsuHPI GGTGA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 18, 246
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 8 cut(s) 207, 306, 309, 559, 588, 607, 610, 631
BccI CCATC 1 cut(s) 403
BceAI ACGGC 1 cut(s) 589
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 2 cut(s) 85, 649
BclI TGATCA 1 cut(s) 564
BfaI CTAG 3 cut(s) 291, 333, 485
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 8 cut(s) 221, 295, 298, 573, 596, 599, 602, 645
BlsI GCNGC 8 cut(s) 222, 296, 299, 574, 597, 600, 603, 646
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 2 cut(s) 85, 649
BmrFI CCNGG 2 cut(s) 85, 649
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 1 cut(s) 631
BmuI ACTGGG 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 400
BpuEI CTTGAG 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 83, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse1I ACTGG 3 cut(s) 112, 191, 460
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 2 cut(s) 85, 649
BseDI CCNNGG 2 cut(s) 83, 307
BseGI GGATG 5 cut(s) 67, 250, 395, 447, 616
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 3 cut(s) 112, 191, 460
BseRI GAGGAG 1 cut(s) 517
BseXI GCAGC 8 cut(s) 207, 306, 309, 559, 588, 607, 610, 631
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 385
BsiHKAI GWGCWC 2 cut(s) 18, 246
BslI CCNNNNNNNGG 1 cut(s) 654
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 1 cut(s) 385
Bsp1286I GDGCHC 2 cut(s) 18, 246
Bsp143I GATC 1 cut(s) 564
Bsp68I TCGCGA 1 cut(s) 48
BspACI CCGC 3 cut(s) 57, 513, 534
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 1 cut(s) 413
BspFNI CGCG 1 cut(s) 48
BsrI ACTGG 3 cut(s) 112, 191, 460
BssECI CCNNGG 2 cut(s) 83, 307
BssMI GATC 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 85, 649
Bst4CI ACNGT 2 cut(s) 311, 561
BstAPI GCANNNNNTGC 1 cut(s) 601
BstDEI CTNAG 1 cut(s) 400
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 5 cut(s) 67, 250, 395, 447, 616
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 1 cut(s) 567
BstMBI GATC 1 cut(s) 564
BstMWI GCNNNNNNNGC 2 cut(s) 54, 601
BstNI CCWGG 2 cut(s) 85, 649
BstSCI CCNGG 2 cut(s) 83, 647
BstSFI CTRYAG 1 cut(s) 255
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 8 cut(s) 207, 306, 309, 559, 588, 607, 610, 631
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 5 cut(s) 67, 250, 395, 447, 616
BtsIMutI CAGTG 3 cut(s) 105, 184, 627
BtuMI TCGCGA 1 cut(s) 48
CaiI CAGNNNCTG 1 cut(s) 601
Csp6I GTAC 1 cut(s) 286
CviJI RGCY 9 cut(s) 16, 164, 172, 190, 220, 300, 385, 495, 601
CviKI_1 RGCY 9 cut(s) 16, 164, 172, 190, 220, 300, 385, 495, 601
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 1 cut(s) 400
DpnI GATC 1 cut(s) 566
DpnII GATC 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoRI GAATTC 2 cut(s) 538, 632
EcoRII CCWGG 2 cut(s) 83, 647
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 18
FaiI YATR 5 cut(s) 351, 357, 388, 390, 432
FauI CCCGC 2 cut(s) 50, 527
FauNDI CATATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 564
Fnu4HI GCNGC 8 cut(s) 221, 295, 298, 573, 596, 599, 602, 645
FokI GGATG 5 cut(s) 74, 237, 382, 434, 623
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 8 cut(s) 221, 295, 298, 573, 596, 599, 602, 645
FspBI CTAG 3 cut(s) 291, 333, 485
GluI GCNGC 8 cut(s) 221, 295, 298, 573, 596, 599, 602, 645
GsuI CTGGAG 1 cut(s) 26
HaeIII GGCC 1 cut(s) 385
HinfI GANTC 3 cut(s) 31, 272, 418
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 362, 472
Hpy188I TCNGA 3 cut(s) 30, 198, 304
Hpy188III TCNNGA 5 cut(s) 47, 157, 281, 333, 465
Hpy8I GTNNAC 2 cut(s) 362, 472
Hpy99I CGWCG 1 cut(s) 641
HpyCH4III ACNGT 2 cut(s) 311, 561
HpyCH4V TGCA 4 cut(s) 223, 233, 575, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 601
HpyF3I CTNAG 1 cut(s) 400
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 1 cut(s) 564
LpnPI CCDG 9 cut(s) 70, 93, 97, 172, 176, 243, 473, 634, 661
Lsp1109I GCAGC 8 cut(s) 207, 306, 309, 559, 588, 607, 610, 631
LweI GCATC 1 cut(s) 631
MaeI CTAG 3 cut(s) 291, 333, 485
MaeIII GTNAC 2 cut(s) 103, 625
MalI GATC 1 cut(s) 566
MboI GATC 1 cut(s) 564
MboII GAAGA 4 cut(s) 62, 71, 134, 281
MhlI GDGCHC 2 cut(s) 18, 246
MluCI AATT 2 cut(s) 538, 632
MmeI TCCRAC 2 cut(s) 572, 588
MnlI CCTC 6 cut(s) 58, 259, 409, 495, 498, 607
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 2 cut(s) 216, 530
MspA1I CMGCKG 1 cut(s) 601
MspR9I CCNGG 2 cut(s) 85, 649
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 2 cut(s) 85, 649
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 54, 601
NdeI CATATG 1 cut(s) 388
NdeII GATC 1 cut(s) 564
NmuCI GTSAC 2 cut(s) 103, 625
NruI TCGCGA 1 cut(s) 48
NsiI ATGCAT 1 cut(s) 235
PctI GAATGC 1 cut(s) 235
PfeI GAWTC 3 cut(s) 31, 272, 418
PkrI GCNGC 8 cut(s) 222, 296, 299, 574, 597, 600, 603, 646
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 83, 647
PspGI CCWGG 2 cut(s) 83, 647
PstNI CAGNNNCTG 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 601
RruI TCGCGA 1 cut(s) 48
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 216, 530
SatI GCNGC 8 cut(s) 221, 295, 298, 573, 596, 599, 602, 645
Sau3AI GATC 1 cut(s) 564
ScaI AGTACT 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 85, 649
SduI GDGCHC 2 cut(s) 18, 246
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 2 cut(s) 538, 632
SsiI CCGC 3 cut(s) 57, 513, 534
SspMI CTAG 3 cut(s) 291, 333, 485
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 2 cut(s) 83, 647
TaaI ACNGT 2 cut(s) 311, 561
TaqI TCGA 4 cut(s) 246, 373, 464, 639
TasI AATT 2 cut(s) 538, 632
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 3 cut(s) 31, 272, 418
Tru1I TTAA 2 cut(s) 216, 530
Tru9I TTAA 2 cut(s) 216, 530
TscAI CASTG 3 cut(s) 112, 191, 634
TseFI GTSAC 2 cut(s) 103, 625
TseI GCWGC 8 cut(s) 220, 294, 297, 572, 595, 598, 601, 644
Tsp45I GTSAC 2 cut(s) 103, 625
TspDTI ATGAA 1 cut(s) 431
TspRI CASTG 3 cut(s) 112, 191, 634
XapI RAATTY 2 cut(s) 538, 632
XbaI TCTAGA 1 cut(s) 332
XspI CTAG 3 cut(s) 291, 333, 485
ZrmI AGTACT 1 cut(s) 287
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.