pycom14g11350

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013309
Physical Location & Seq
Reverse (-)
14414036 .. 14415712
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1278 bp
ATGGCGGCCACCACCAGCTTCCCCTACCCCTCTGCAATTTTCCTGTCAGTCCTCATCATTTCAGGGTTCAAGTTTCCAGAAACGGTAAATTCAATGGGAATAAACTACGGCCAAATCGCCAACAACTTACCGTCACCGGACGACGTCGTTCCGCTGGTGAAGTCCATCGGCATCTCCAAGATCAAGCTGTACGACGCCGATCCCAAAGTCCTCAAAGCCTTCGCCAACACTGGCGTCGAGTTCATAGTGGGCCTCCCCAACGAGTACCTCTCCAAGGTCCAGGCCCCGGCCCAGGCCCAATCCTGGGTCAAGTCCAACGTCCAGGCCCACCTCCCCGCCACCAACATCACAAGCATCTTCGTCGGCAACGAGGTCCTCACCCTCAACGACACGTCGTTGAGCAACAACCTCCTTCCGGCAATGCAGAGCGTCCACTCCGCCCTCGTTGGTCTAGGCCTCGATAAGCAAGTCGCCGTCACCACCGCCCACTCCCTCTCCATTCTCGAGACCTCGTTTCCGCCCTCCGCCGGCGCTTTCCGCCGAGATCTGACTAGCTGCATCACTCCGATCCTCAATTTCCACGCCAAAACCGGATCGCCATTCCTGATCAATGCCTACCCTTACTTCGCCTACAAGTCGAACCCAAAGCAGGTGCCTCTCGACTACGTCCTCTTCCAGCCGAACTCTGGCTCCGTCGATCCCGCCACCAATCTCCACTATGACAACATGCTCATCGCCCAGATCGACGCCGTGTACTCCGCCCTGGGCTCCCTCGGTTTGAAGAAGCTCCCGGTCCACATCTCCGAGACCGGGTGGCCTTCGAAGGGAGACGAGGACGAGGCCGGAGCGACGCCGGAGAACGCGAAGAAGTACAACGGGAATCTGATCAAGCTCATTTACCAGAAGAAGGGAACGCCGCTCAGGCCCGGGTCGGATCTCAACATCTACGTCTTCGCTCTGTTCAACGAGAACATGAAACCCGGCCCGAGTTCAGAGAGGAATTTCGGGCTTTTTAAACCCGACGGGTCGCCTGCGTACTCAATCGGGCTCGCGGCGGGCAACATTTCCTTTGGTAACTCCAGTTCGAGTTCGGGTTCTGGTTCGAGTTCGGGCCAGGGACAGGTGAACCCGATTAGTAACGGTAGGTCTCCGTCGGATTTTTCCGGCACGCCATACTTGTCCATTTCCTCCGCCCCGGCCACGGAGAGATGGGATGTGATTGGTGGGATTCCGATGACGTGGCTAGTGGTCTCGTTGTTGATGAAACACGTCCCTTGA

Protein Analysis

426

Amino Acids

45.23

Weight (kDa)

7.06

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 32 - 348 1.6e-86 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 640
AatII GACGTC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 640
AccB1I GGYRCC 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 919
AccII CGCG 2 cut(s) 863, 1052
AclWI GGATC 5 cut(s) 194, 562, 601, 692, 942
AcoI YGGCCR 3 cut(s) 6, 109, 1197
AcsI RAATTY 2 cut(s) 88, 1000
AcyI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
AfaI GTAC 5 cut(s) 191, 266, 755, 872, 1037
AflIII ACRYGT 2 cut(s) 390, 1267
AgsI TTSAA 4 cut(s) 70, 93, 781, 964
AjiI CACGTC 3 cut(s) 393, 1239, 1270
AjnI CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1113
AloI GAACNNNNNNTCC 2 cut(s) 674, 706
AluBI AGCT 5 cut(s) 18, 187, 555, 787, 892
AluI AGCT 5 cut(s) 18, 187, 555, 787, 892
Alw26I GTCTC 5 cut(s) 500, 800, 822, 1152, 1255
AlwI GGATC 5 cut(s) 194, 562, 601, 692, 942
Ama87I CYCGRG 3 cut(s) 503, 926, 985
ApeKI GCWGC 1 cut(s) 555
ApoI RAATTY 2 cut(s) 88, 1000
AspLEI GCGC 1 cut(s) 533
AsuC2I CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1196
AsuHPI GGTGA 5 cut(s) 126, 169, 370, 469, 1135
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 3 cut(s) 503, 926, 985
AvaII GGWCC 3 cut(s) 277, 373, 793
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 2 cut(s) 770, 1050
BbsI GAAGAC 1 cut(s) 943
BbvI GCAGC 1 cut(s) 542
BccI CCATC 2 cut(s) 173, 1203
BceAI ACGGC 3 cut(s) 124, 458, 734
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1115
BclI TGATCA 2 cut(s) 606, 885
BcnI CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1196
BcoDI GTCTC 5 cut(s) 500, 800, 822, 1152, 1255
BfaI CTAG 3 cut(s) 452, 552, 1244
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 640
BglI GCCNNNNNGGC 1 cut(s) 922
BglII AGATCT 1 cut(s) 544
BisI GCNGC 4 cut(s) 6, 556, 917, 1053
BlsI GCNGC 4 cut(s) 7, 557, 918, 1054
Bme18I GGWCC 3 cut(s) 277, 373, 793
BmeT110I CYCGRG 3 cut(s) 503, 926, 985
BmgBI CACGTC 3 cut(s) 393, 1239, 1270
BmiI GGNNCC 4 cut(s) 285, 654, 691, 769
BmsI GCATC 3 cut(s) 180, 363, 567
BpiI GAAGAC 1 cut(s) 943
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 1 cut(s) 1063
Bpu10I CCTNAGC 1 cut(s) 920
Bpu14I TTCGAA 1 cut(s) 821
BpuMI CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1196
BsaHI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BsaI GGTCTC 4 cut(s) 500, 800, 1152, 1255
BsaWI WCCGGW 2 cut(s) 136, 590
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 674, 704
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 2 cut(s) 235, 1080
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1115
BseGI GGATG 1 cut(s) 1219
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 934
BseNI ACTGG 2 cut(s) 235, 1080
BseXI GCAGC 1 cut(s) 542
Bsh1236I CGCG 2 cut(s) 863, 1052
BshNI GGYRCC 1 cut(s) 652
BsiHKCI CYCGRG 3 cut(s) 503, 926, 985
BslFI GGGAC 2 cut(s) 1131, 1256
BsmAI GTCTC 5 cut(s) 500, 800, 822, 1152, 1255
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 2 cut(s) 1131, 1256
Bso31I GGTCTC 4 cut(s) 500, 800, 1152, 1255
BsoBI CYCGRG 3 cut(s) 503, 926, 985
Bsp119I TTCGAA 1 cut(s) 821
Bsp1286I GDGCHC 2 cut(s) 770, 1050
BspCNI CTCAG 1 cut(s) 933
BspFNI CGCG 2 cut(s) 863, 1052
BspLI GGNNCC 4 cut(s) 285, 654, 691, 769
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 5 cut(s) 194, 562, 601, 692, 942
BspT104I TTCGAA 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 652
BspTNI GGTCTC 4 cut(s) 500, 800, 1152, 1255
BsrBI CCGCTC 1 cut(s) 919
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 2 cut(s) 235, 1080
BssAI RCCGGY 1 cut(s) 527
BssNI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1115
Bst4CI ACNGT 3 cut(s) 85, 132, 1142
Bst6I CTCTTC 1 cut(s) 677
BstACI GRCGYC 5 cut(s) 144, 195, 234, 747, 851
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 5 cut(s) 529, 1032, 1050, 1057, 1169
BstDEI CTNAG 1 cut(s) 920
BstDSI CCRYGG 1 cut(s) 1200
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 1219
BstFNI CGCG 2 cut(s) 863, 1052
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstMAI GTCTC 5 cut(s) 500, 800, 822, 1152, 1255
BstMWI GCNNNNNNNGC 2 cut(s) 537, 922
BstNI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1115
BstNSI RCATGY 1 cut(s) 730
BstUI CGCG 2 cut(s) 863, 1052
BstV1I GCAGC 1 cut(s) 542
BstV2I GAAGAC 1 cut(s) 943
BstX2I RGATCY 2 cut(s) 544, 934
BstYI RGATCY 2 cut(s) 544, 934
BtgI CCRYGG 1 cut(s) 1200
BtgZI GCGATG 1 cut(s) 718
BtrI CACGTC 3 cut(s) 393, 1239, 1270
BtsCI GGATG 1 cut(s) 1219
BtsIMutI CAGTG 1 cut(s) 228
BveI ACCTGC 1 cut(s) 640
Cac8I GCNNGC 5 cut(s) 529, 1032, 1050, 1057, 1169
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 1 cut(s) 527
Cfr9I CCCGGG 1 cut(s) 926
CseI GACGC 5 cut(s) 203, 223, 418, 755, 859
Csp6I GTAC 5 cut(s) 190, 265, 754, 871, 1036
CviAII CATG 2 cut(s) 727, 973
CviQI GTAC 5 cut(s) 190, 265, 754, 871, 1036
DdeI CTNAG 1 cut(s) 920
DraI TTTAAA 1 cut(s) 1015
EaeI YGGCCR 3 cut(s) 6, 109, 1197
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
EciI GGCGGA 6 cut(s) 427, 507, 514, 527, 748, 1180
Eco130I CCWWGG 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 770, 1050
Eco31I GGTCTC 4 cut(s) 500, 800, 1152, 1255
Eco47I GGWCC 3 cut(s) 277, 373, 793
Eco88I CYCGRG 3 cut(s) 503, 926, 985
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 283, 373
EcoRII CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1113
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 770, 1050
ErhI CCWWGG 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 822
FaeI CATG 2 cut(s) 730, 976
FaiI YATR 5 cut(s) 245, 720, 728, 974, 1174
FaqI GGGAC 2 cut(s) 1131, 1256
FatI CATG 2 cut(s) 726, 972
FauI CCCGC 3 cut(s) 343, 709, 1048
FbaI TGATCA 2 cut(s) 606, 885
Fnu4HI GCNGC 4 cut(s) 6, 556, 917, 1053
FokI GGATG 1 cut(s) 1226
FriOI GRGCYC 2 cut(s) 770, 1050
Fsp4HI GCNGC 4 cut(s) 6, 556, 917, 1053
FspBI CTAG 3 cut(s) 452, 552, 1244
GlaI GCGC 1 cut(s) 532
GluI GCNGC 4 cut(s) 6, 556, 917, 1053
GsuI CTGGAG 1 cut(s) 1063
HaeII RGCGCY 1 cut(s) 534
HgaI GACGC 5 cut(s) 203, 223, 418, 755, 859
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 5 cut(s) 144, 195, 234, 747, 851
Hin1II CATG 2 cut(s) 730, 976
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HinfI GANTC 2 cut(s) 880, 1228
HphI GGTGA 5 cut(s) 126, 169, 370, 469, 1135
Hpy166II GTNNAC 4 cut(s) 433, 754, 796, 1126
Hpy188I TCNGA 8 cut(s) 549, 567, 805, 885, 934, 994, 1156, 1233
Hpy188III TCNNGA 5 cut(s) 77, 503, 505, 604, 659
Hpy8I GTNNAC 4 cut(s) 433, 754, 796, 1126
HpyAV CCTTC 5 cut(s) 229, 422, 817, 828, 901
HpyCH4III ACNGT 3 cut(s) 85, 132, 1142
HpyCH4IV ACGT 7 cut(s) 144, 318, 392, 666, 948, 1238, 1269
HpyCH4V TGCA 3 cut(s) 35, 424, 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 537, 922
HpyF3I CTNAG 1 cut(s) 920
HpySE526I ACGT 7 cut(s) 144, 318, 392, 666, 948, 1238, 1269
Hsp92I GRCGYC 5 cut(s) 144, 195, 234, 747, 851
Hsp92II CATG 2 cut(s) 730, 976
HspAI GCGC 1 cut(s) 531
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 606, 885
LmnI GCTCC 4 cut(s) 695, 773, 792, 845
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 3 cut(s) 180, 363, 567
MaeI CTAG 3 cut(s) 452, 552, 1244
MaeII ACGT 7 cut(s) 144, 318, 392, 666, 948, 1238, 1269
MaeIII GTNAC 4 cut(s) 132, 475, 1073, 1136
MbiI CCGCTC 1 cut(s) 919
MboII GAAGA 6 cut(s) 349, 664, 793, 877, 916, 943
MflI RGATCY 2 cut(s) 544, 934
MhlI GDGCHC 2 cut(s) 770, 1050
MluCI AATT 4 cut(s) 36, 88, 574, 1000
MmeI TCCRAC 3 cut(s) 339, 912, 1134
MreI CGCCGGCG 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 527
MseI TTAA 1 cut(s) 1014
MspA1I CMGCKG 1 cut(s) 154
MvaI CCWGG 6 cut(s) 281, 293, 304, 323, 764, 1115
MvnI CGCG 2 cut(s) 863, 1052
MwoI GCNNNNNNNGC 2 cut(s) 537, 922
NaeI GCCGGC 1 cut(s) 529
NciI CCSGG 7 cut(s) 287, 791, 811, 927, 928, 981, 1196
NgoMIV GCCGGC 1 cut(s) 527
NlaIII CATG 2 cut(s) 730, 976
NlaIV GGNNCC 4 cut(s) 285, 654, 691, 769
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 2 cut(s) 132, 475
NspI RCATGY 1 cut(s) 730
NspV TTCGAA 1 cut(s) 821
PaeR7I CTCGAG 1 cut(s) 503
PaqCI CACCTGC 1 cut(s) 640
PasI CCCWGGG 1 cut(s) 763
PceI AGGCCT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 2 cut(s) 366, 741
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 880, 1228
PflFI GACNNNGTC 2 cut(s) 143, 665
PkrI GCNGC 4 cut(s) 7, 557, 918, 1054
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1113
PspGI CCWGG 6 cut(s) 279, 291, 302, 321, 762, 1113
PspN4I GGNNCC 4 cut(s) 285, 654, 691, 769
PspPPI RGGWCCY 1 cut(s) 373
PsrI GAACNNNNNNTAC 2 cut(s) 1066, 1098
PsuI RGATCY 2 cut(s) 544, 934
PsyI GACNNNGTC 2 cut(s) 143, 665
RsaI GTAC 5 cut(s) 191, 266, 755, 872, 1037
RsaNI GTAC 5 cut(s) 190, 265, 754, 871, 1036
SaqAI TTAA 1 cut(s) 1014
SatI GCNGC 4 cut(s) 6, 556, 917, 1053
SduI GDGCHC 2 cut(s) 770, 1050
SfaNI GCATC 3 cut(s) 180, 363, 567
Sfr274I CTCGAG 1 cut(s) 503
SfuI TTCGAA 1 cut(s) 821
SgrAI CRCCGGYG 1 cut(s) 527
SinI GGWCC 3 cut(s) 277, 373, 793
SlaI CTCGAG 1 cut(s) 503
SmaI CCCGGG 1 cut(s) 928
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 4 cut(s) 36, 88, 574, 1000
SseBI AGGCCT 1 cut(s) 456
SspMI CTAG 3 cut(s) 452, 552, 1244
StuI AGGCCT 1 cut(s) 456
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 85, 132, 1142
TaiI ACGT 7 cut(s) 147, 321, 395, 669, 951, 1241, 1272
TasI AATT 4 cut(s) 36, 88, 574, 1000
TatI WGTACW 2 cut(s) 753, 870
TauI GCSGC 3 cut(s) 8, 919, 1055
TfiI GAWTC 2 cut(s) 880, 1228
Tru1I TTAA 1 cut(s) 1014
Tru9I TTAA 1 cut(s) 1014
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 2 cut(s) 132, 475
TseI GCWGC 1 cut(s) 555
Tsp45I GTSAC 2 cut(s) 132, 475
TspDTI ATGAA 3 cut(s) 232, 989, 1277
TspGWI ACGGA 3 cut(s) 682, 1140, 1217
TspMI CCCGGG 1 cut(s) 926
TspRI CASTG 1 cut(s) 235
Tth111I GACNNNGTC 2 cut(s) 143, 665
VpaK11BI GGWCC 3 cut(s) 277, 373, 793
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 2 cut(s) 88, 1000
XceI RCATGY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 683
XhoI CTCGAG 1 cut(s) 503
XmaI CCCGGG 1 cut(s) 926
XspI CTAG 3 cut(s) 452, 552, 1244
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.