pycom14g12220

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007591
Physical Location & Seq
Reverse (-)
15257611 .. 15261822
4212 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1587 bp
ATGGCGGAAGAGGAGAACGGCTATCCAGATAGCAGGCAAGAGCAAGAAGAAGCCCTCGTCGCTCTGATCGAGCATCGCACCCGGGAAGTCCACCATCTCCGCCAGCGCGTCGACTATTACAAAACTCAGCTTGGAGAAACGGAGAAGAGGTTAACTGATACCGAAATTAAGTTGGCTCGACTTCGACGCCAAGACAAAGTAGGGTCAACCAGGAGTTCTCCGGGTGGTGGAACGAAAAGTGCAAAGGTGGAGCGCAGATCAACAAGTCCTATACATTTAAATGAAGGTTCTTCTAGACACCAGCATCAATCAAAAACAGAACTTTTCATTCCTGCAGTCGGCCCAAAAGTTTCCCAACCTACAATGGTGGCAGGGTCTAGTGGGAAAAATCCAGTTAATTATAGTGCTCAAGCAAGTCCATCAGTTTCTTGTCAGTCTATGAGTGCTAACAGAACGAAAGGTGACAAATTGTATAGAAATTCTTCAAAGCAGGACGTTGAAGTTCAAGATAATGGGAAAAAACGGAAATTTGAACAGAAAGAACACAAAGAATTAGTCTCGTTGGTTCGCACTAGCTCTCAACCATGTAAAATCAACTGCTATGCGAGCAGTCACATCTCAAGTCAGCACAAGAGAAAGTTGAGAAGTCTTTCTTTGTGTCCAGTGAACGATCAGCTTTTTGTGACCAGTGCTTTGGATGGAGTAGTCAACTTCTGGCAAGTTCAGTCCAAGGGGTCTAGTGCTTCTCGACTTAGTTCTACTGATTGTGCATCTCCAAACAGGAGATGGCCAGAAGATATAGCCTGGCACCCTGATGGAAACAGACTATTTTCTGTGTATAGTGCTGATGGTGGCGACTCGCAAGTATCAGTCCTAGATTTAAATATAACACAAGGGAAAGGTCGTGTTACTTTCATCAAAGATAAACCTCATGTGAGAGGGATCATTAACAACATAGTTTTCCCACCCTGGGAGGATACCTGTTTTGTCACTGGTGGTAGTGATCATGCTGTTATAATCTGGAAAGAGGCTGATGCAGAGAATGTATGGAAACCAAAGACACTGCATAGGAATATGCACTCATCTGCTGTTATGGGAGTTGCCGGGATGTTCCAAAAGCAGATAATACTATCAGCCGGAGCAGACAAGAGAATTGTTGGGTTTGACGCTAACACAGGAAGAGCAGAGTTTAAACATCAGATAGAAAGTAAATGCATGAGTGTCTTGCCAAATCCTTGCGACTTCAATATCTTCATGGTTCAATCAGGGACCCATGAGAAGCAGCTCCGACTATTTGATATCAGACTGAGGCAAACAGAGATACATGCTTTTGGGTGGAAGCAAGAGAGCAGTGAATCTCAGTCAGCTCTGATCAATCAGTCGTGGTCTCCTGATGGCTTATACATCTCATCTGGTACAGCAGATCCATTGATCCACATATTCGATATCATATACAACGCCAACAAGCCTTCTCAATCTATAAGCGCTCATCAGAAACGGGTATTCAAAGCTGTATGGCTCCACTCATGCCCCCTTCTCATTTCCATATCTTCTGATTTGAACATTGGTTTACACAAAACCACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

529

Amino Acids

59.0

Weight (kDa)

9.26

Isoelectric Point (pI)

64.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 205 - 393 2.1e-12 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 208 - 435 5.5e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 284 - 483 2.9e-10 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 360 - 520 5.9e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 365 - 523 3.6e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 420 - 526 2.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AccB1I GGYRCC 1 cut(s) 807
AccI GTMKAC 1 cut(s) 111
AccII CGCG 1 cut(s) 108
AciI CCGC 2 cut(s) 5, 100
AclWI GGATC 3 cut(s) 950, 1418, 1426
AcoI YGGCCR 1 cut(s) 788
AcsI RAATTY 2 cut(s) 478, 527
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 1 cut(s) 1417
AfeI AGCGCT 1 cut(s) 1486
AfiI CCNNNNNNNGG 3 cut(s) 227, 338, 970
AgsI TTSAA 8 cut(s) 486, 500, 506, 533, 1246, 1262, 1507, 1561
AjnI CCWGG 3 cut(s) 209, 803, 968
AluBI AGCT 6 cut(s) 130, 576, 676, 1285, 1367, 1511
AluI AGCT 6 cut(s) 130, 576, 676, 1285, 1367, 1511
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 2 cut(s) 562, 1392
AlwI GGATC 3 cut(s) 950, 1418, 1426
Ama87I CYCGRG 1 cut(s) 81
Aor51HI AGCGCT 1 cut(s) 1486
AoxI GGCC 2 cut(s) 340, 788
ApeKI GCWGC 1 cut(s) 1282
ApoI RAATTY 2 cut(s) 478, 527
ArsI GACNNNNNNTTYG 4 cut(s) 540, 572, 676, 708
Asp700I GAANNNNTTC 2 cut(s) 481, 649
AspLEI GCGC 3 cut(s) 108, 255, 1487
AspS9I GGNCC 2 cut(s) 341, 1269
AsuC2I CCSGG 4 cut(s) 82, 83, 222, 1105
AsuHPI GGTGA 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 1 cut(s) 1269
BalI TGGCCA 1 cut(s) 790
BanI GGYRCC 1 cut(s) 807
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 1294
BccI CCATC 7 cut(s) 102, 427, 692, 780, 809, 842, 1388
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 3 cut(s) 211, 805, 970
BciVI GTATCC 1 cut(s) 970
BclI TGATCA 2 cut(s) 1003, 1371
BcnI CCSGG 4 cut(s) 82, 83, 222, 1105
BcoDI GTCTC 2 cut(s) 562, 1392
BfaI CTAG 5 cut(s) 294, 378, 573, 738, 875
BfmI CTRYAG 1 cut(s) 333
BfoI RGCGCY 1 cut(s) 1488
BfuI GTATCC 1 cut(s) 970
BisI GCNGC 1 cut(s) 1283
BlsI GCNGC 1 cut(s) 1284
Bme1390I CCNGG 7 cut(s) 82, 83, 211, 222, 805, 970, 1105
Bme18I GGWCC 1 cut(s) 1269
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 341, 1269
BmiI GGNNCC 4 cut(s) 809, 1270, 1271, 1520
BmrFI CCNGG 7 cut(s) 82, 83, 211, 222, 805, 970, 1105
BmsI GCATC 4 cut(s) 82, 313, 779, 1024
BpuEI CTTGAG 2 cut(s) 393, 604
BpuMI CCSGG 4 cut(s) 82, 83, 222, 1105
BsaAI YACGTR 1 cut(s) 1584
BsaHI GRCGYC 1 cut(s) 187
BsaI GGTCTC 1 cut(s) 1392
BsaJI CCNNGG 4 cut(s) 81, 729, 968, 969
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 338, 970
Bse1I ACTGG 4 cut(s) 392, 662, 687, 997
BseBI CCWGG 3 cut(s) 211, 805, 970
BseDI CCNNGG 4 cut(s) 81, 729, 968, 969
BseGI GGATG 2 cut(s) 703, 1113
BseLI CCNNNNNNNGG 3 cut(s) 227, 338, 970
BseMII CTCAG 3 cut(s) 140, 1298, 1373
BseNI ACTGG 4 cut(s) 392, 662, 687, 997
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 1294
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 342, 790
BshNI GGYRCC 1 cut(s) 807
BsiHKAI GWGCWC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 4 cut(s) 82, 221, 1104, 1137
BslFI GGGAC 1 cut(s) 1282
BslI CCNNNNNNNGG 3 cut(s) 227, 338, 970
BsmAI GTCTC 2 cut(s) 562, 1392
BsmFI GGGAC 1 cut(s) 1282
BsnI GGCC 2 cut(s) 342, 790
Bso31I GGTCTC 1 cut(s) 1392
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
BspACI CCGC 2 cut(s) 5, 100
BspANI GGCC 2 cut(s) 342, 790
BspCNI CTCAG 3 cut(s) 139, 1299, 1372
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 4 cut(s) 809, 1270, 1271, 1520
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 3 cut(s) 950, 1418, 1426
BspQI GCTCTTC 1 cut(s) 1174
BspT107I GGYRCC 1 cut(s) 807
BspTNI GGTCTC 1 cut(s) 1392
BsrI ACTGG 4 cut(s) 392, 662, 687, 997
BssECI CCNNGG 4 cut(s) 81, 729, 968, 969
BssMI GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
BssNI GRCGYC 1 cut(s) 187
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 3 cut(s) 211, 805, 970
Bst6I CTCTTC 3 cut(s) 3, 140, 1174
BstACI GRCGYC 1 cut(s) 187
BstBAI YACGTR 1 cut(s) 1584
BstC8I GCNNGC 3 cut(s) 35, 104, 607
BstDEI CTNAG 4 cut(s) 126, 752, 1307, 1359
BstF5I GGATG 2 cut(s) 703, 1113
BstFNI CGCG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 1488
BstHHI GCGC 3 cut(s) 108, 255, 1487
BstKTI GATC 8 cut(s) 69, 260, 673, 945, 1006, 1374, 1426, 1434
BstMAI GTCTC 2 cut(s) 562, 1392
BstMBI GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
BstMWI GCNNNNNNNGC 2 cut(s) 59, 606
BstNI CCWGG 3 cut(s) 211, 805, 970
BstNSI RCATGY 1 cut(s) 1328
BstSCI CCNGG 7 cut(s) 80, 81, 209, 220, 803, 968, 1103
BstSFI CTRYAG 1 cut(s) 333
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 1294
BstX2I RGATCY 1 cut(s) 1423
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 1 cut(s) 1423
BsuI GTATCC 1 cut(s) 970
BsuRI GGCC 2 cut(s) 342, 790
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 2 cut(s) 703, 1113
BtsI GCAGTG 2 cut(s) 1061, 1357
BtsIMutI CAGTG 5 cut(s) 669, 694, 990, 1061, 1357
Cac8I GCNNGC 3 cut(s) 35, 104, 607
CfoI GCGC 3 cut(s) 108, 255, 1487
Cfr13I GGNCC 2 cut(s) 341, 1269
Cfr9I CCCGGG 1 cut(s) 81
CseI GACGC 3 cut(s) 97, 195, 1175
Csp6I GTAC 1 cut(s) 1416
CviAII CATG 8 cut(s) 585, 932, 1007, 1216, 1255, 1274, 1325, 1527
CviQI GTAC 1 cut(s) 1416
DdeI CTNAG 4 cut(s) 126, 752, 1307, 1359
DpnI GATC 8 cut(s) 68, 259, 672, 944, 1005, 1373, 1425, 1433
DpnII GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
DraI TTTAAA 3 cut(s) 279, 882, 1192
EaeI YGGCCR 1 cut(s) 788
Eam1104I CTCTTC 3 cut(s) 3, 140, 1174
EarI CTCTTC 3 cut(s) 3, 140, 1174
EciI GGCGGA 2 cut(s) 20, 89
Eco130I CCWWGG 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 1392
Eco32I GATATC 2 cut(s) 1300, 1447
Eco47I GGWCC 1 cut(s) 1269
Eco47III AGCGCT 1 cut(s) 1486
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 1269
EcoRII CCWGG 3 cut(s) 209, 803, 968
EcoRV GATATC 2 cut(s) 1300, 1447
EcoT14I CCWWGG 1 cut(s) 729
EcoT22I ATGCAT 1 cut(s) 1217
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 8 cut(s) 588, 935, 1010, 1219, 1258, 1277, 1328, 1530
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FaqI GGGAC 1 cut(s) 1282
FatI CATG 8 cut(s) 584, 931, 1006, 1215, 1254, 1273, 1324, 1526
FbaI TGATCA 2 cut(s) 1003, 1371
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 1283
FokI GGATG 2 cut(s) 710, 1120
Fsp4HI GCNGC 1 cut(s) 1283
FspBI CTAG 5 cut(s) 294, 378, 573, 738, 875
GlaI GCGC 3 cut(s) 107, 254, 1486
GluI GCNGC 1 cut(s) 1283
HaeII RGCGCY 1 cut(s) 1488
HaeIII GGCC 2 cut(s) 342, 790
HapII CCGG 4 cut(s) 82, 221, 1104, 1137
HgaI GACGC 3 cut(s) 97, 195, 1175
HhaI GCGC 3 cut(s) 108, 255, 1487
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 8 cut(s) 588, 935, 1010, 1219, 1258, 1277, 1328, 1530
Hin6I GCGC 3 cut(s) 106, 253, 1485
HinP1I GCGC 3 cut(s) 106, 253, 1485
HincII GTYRAC 4 cut(s) 112, 153, 207, 709
HindII GTYRAC 4 cut(s) 112, 153, 207, 709
HinfI GANTC 2 cut(s) 857, 1355
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 4 cut(s) 82, 221, 1104, 1137
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 7 cut(s) 91, 112, 153, 207, 667, 709, 1571
Hpy188I TCNGA 7 cut(s) 66, 1200, 1289, 1304, 1371, 1494, 1555
Hpy188III TCNNGA 6 cut(s) 26, 294, 506, 747, 1021, 1391
Hpy8I GTNNAC 7 cut(s) 91, 112, 153, 207, 667, 709, 1571
Hpy99I CGWCG 3 cut(s) 62, 113, 189
HpyAV CCTTC 3 cut(s) 278, 1479, 1544
HpyCH4IV ACGT 2 cut(s) 495, 1583
HpyCH4V TGCA 7 cut(s) 242, 335, 770, 1037, 1066, 1078, 1215
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 606
HpyF3I CTNAG 4 cut(s) 126, 752, 1307, 1359
HpySE526I ACGT 2 cut(s) 495, 1583
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 8 cut(s) 588, 935, 1010, 1219, 1258, 1277, 1328, 1530
HspAI GCGC 3 cut(s) 106, 253, 1485
KflI GGGWCCC 1 cut(s) 1269
Ksp22I TGATCA 2 cut(s) 1003, 1371
KspAI GTTAAC 1 cut(s) 153
Kzo9I GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
LguI GCTCTTC 1 cut(s) 1174
LmnI GCTCC 4 cut(s) 250, 1139, 1290, 1524
Lsp1109I GCAGC 1 cut(s) 1294
LweI GCATC 4 cut(s) 82, 313, 779, 1024
MaeI CTAG 5 cut(s) 294, 378, 573, 738, 875
MaeII ACGT 2 cut(s) 495, 1583
MaeIII GTNAC 5 cut(s) 461, 611, 682, 907, 988
MalI GATC 8 cut(s) 68, 259, 672, 944, 1005, 1373, 1425, 1433
MboI GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
MboII GAAGA 9 cut(s) 20, 59, 157, 282, 474, 806, 1191, 1243, 1542
MflI RGATCY 1 cut(s) 1423
MhlI GDGCHC 1 cut(s) 409
MlsI TGGCCA 1 cut(s) 790
MluCI AATT 7 cut(s) 165, 397, 467, 478, 527, 551, 1152
MluNI TGGCCA 1 cut(s) 790
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 1 cut(s) 1312
MnlI CCTC 8 cut(s) 4, 65, 141, 932, 939, 967, 1021, 1302
Mox20I TGGCCA 1 cut(s) 790
Mph1103I ATGCAT 1 cut(s) 1217
MroXI GAANNNNTTC 2 cut(s) 481, 649
MscI TGGCCA 1 cut(s) 790
MseI TTAA 7 cut(s) 152, 168, 278, 396, 881, 948, 1191
MslI CAYNNNNRTG 2 cut(s) 279, 813
Msp20I TGGCCA 1 cut(s) 790
MspI CCGG 4 cut(s) 82, 221, 1104, 1137
MspR9I CCNGG 7 cut(s) 82, 83, 211, 222, 805, 970, 1105
MssI GTTTAAAC 1 cut(s) 1192
MvaI CCWGG 3 cut(s) 211, 805, 970
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 59, 606
NciI CCSGG 4 cut(s) 82, 83, 222, 1105
NdeII GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
NlaIII CATG 8 cut(s) 588, 935, 1010, 1219, 1258, 1277, 1328, 1530
NlaIV GGNNCC 4 cut(s) 809, 1270, 1271, 1520
NmuCI GTSAC 4 cut(s) 461, 611, 682, 988
NsiI ATGCAT 1 cut(s) 1217
NspI RCATGY 1 cut(s) 1328
PasI CCCWGGG 1 cut(s) 969
PciSI GCTCTTC 1 cut(s) 1174
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 2 cut(s) 481, 649
PfeI GAWTC 1 cut(s) 1355
PkrI GCNGC 1 cut(s) 1284
PleI GAGTC 1 cut(s) 851
PmeI GTTTAAAC 1 cut(s) 1192
PpsI GAGTC 1 cut(s) 851
Ppu21I YACGTR 1 cut(s) 1584
PpuMI RGGWCCY 1 cut(s) 1269
PsiI TTATAA 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 1269
Psp6I CCWGG 3 cut(s) 209, 803, 968
PspGI CCWGG 3 cut(s) 209, 803, 968
PspN4I GGNNCC 4 cut(s) 809, 1270, 1271, 1520
PspPI GGNCC 2 cut(s) 341, 1269
PspPPI RGGWCCY 1 cut(s) 1269
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 1423
RsaI GTAC 1 cut(s) 1417
RsaNI GTAC 1 cut(s) 1416
RseI CAYNNNNRTG 2 cut(s) 279, 813
SalI GTCGAC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 1174
SaqAI TTAA 7 cut(s) 152, 168, 278, 396, 881, 948, 1191
SatI GCNGC 1 cut(s) 1283
Sau3AI GATC 8 cut(s) 66, 257, 670, 942, 1003, 1371, 1423, 1431
Sau96I GGNCC 2 cut(s) 341, 1269
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 7 cut(s) 82, 83, 211, 222, 805, 970, 1105
SduI GDGCHC 1 cut(s) 409
SfaNI GCATC 4 cut(s) 82, 313, 779, 1024
SfcI CTRYAG 1 cut(s) 333
SinI GGWCC 1 cut(s) 1269
SmaI CCCGGG 1 cut(s) 83
SmiI ATTTAAAT 2 cut(s) 279, 882
SmiMI CAYNNNNRTG 2 cut(s) 279, 813
SmlI CTYRAG 2 cut(s) 408, 619
SmoI CTYRAG 2 cut(s) 408, 619
Sse9I AATT 7 cut(s) 165, 397, 467, 478, 527, 551, 1152
SsiI CCGC 2 cut(s) 5, 100
SspMI CTAG 5 cut(s) 294, 378, 573, 738, 875
StyD4I CCNGG 7 cut(s) 80, 81, 209, 220, 803, 968, 1103
StyI CCWWGG 1 cut(s) 729
SwaI ATTTAAAT 2 cut(s) 279, 882
TaiI ACGT 2 cut(s) 498, 1586
TaqI TCGA 6 cut(s) 69, 111, 178, 184, 748, 1443
TasI AATT 7 cut(s) 165, 397, 467, 478, 527, 551, 1152
TfiI GAWTC 1 cut(s) 1355
Tru1I TTAA 7 cut(s) 152, 168, 278, 396, 881, 948, 1191
Tru9I TTAA 7 cut(s) 152, 168, 278, 396, 881, 948, 1191
TscAI CASTG 5 cut(s) 669, 694, 997, 1068, 1357
TseFI GTSAC 4 cut(s) 461, 611, 682, 988
TseI GCWGC 1 cut(s) 1282
Tsp45I GTSAC 4 cut(s) 461, 611, 682, 988
TspDTI ATGAA 4 cut(s) 297, 316, 904, 1243
TspGWI ACGGA 2 cut(s) 155, 538
TspMI CCCGGG 1 cut(s) 81
TspRI CASTG 5 cut(s) 669, 694, 997, 1068, 1357
VpaK11BI GGWCC 1 cut(s) 1269
XapI RAATTY 2 cut(s) 478, 527
XbaI TCTAGA 1 cut(s) 293
XceI RCATGY 1 cut(s) 1328
XcmI CCANNNNNNNNNTGG 1 cut(s) 783
XmaI CCCGGG 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 2 cut(s) 481, 649
XspI CTAG 5 cut(s) 294, 378, 573, 738, 875
Zsp2I ATGCAT 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.