pycom14g12630

Responsible for synthesis of pseudouridine from uracil

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009164
Physical Location & Seq
Reverse (-)
15613823 .. 15616966
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGAAGAGGACGTGGGAAGACAAGGCGATGGAGATCGTGTGGCAGAGTCCAGCGATTCGGCCGCAACCGCAAGATTACATCTTTCGCGACGGGAGGCGCCATGTCAGACCCTACTACTTCGAGTTCATCTGTCATGTGAAGAATCGGTGGGCGGGGAAGACCATCGTTGATTTGTTCACTCATGAGTTCAAAGGCCGGCCTTATGATTACTATGTTAGTGCAGTGAAATCTGGAAGGATACAGGTTGATGGAGAGATGGTGCCGGTTTCCCACATTGTTAAAACGTCGCAAAAGATAAGCCACTTCGTCCACAGGCATGAACCCCCAGTCATGACTTGGGATATATCGATTCTTCAAGAAAAACCGGATGTGGTTGTTGTTTGTAAGCCTGCATCTGTTCCGGTGCATCCGTGTGGTCAATATCGTAAGAACACGGTTGTTGGCATCCTTGAGGCTGAGCATGGCTTGGCACCTCTATTTCCAATTCATCGACTAGATCGCCTTGTCTCAGGACTCCTTATCTTGGCCAGAAGTGCTTCAAAAGCCGACTATTTTAGGCAGCAGATTGAAGGAGGTATGGTCCAGAAACAGTACATTGCAAGAGTTGCTGGGGAATTTCCTGAGCAAGAGCAAGTTGTAGATGCTAATGTTAATCATGATGCTCGAGAATGTAGGAGCACAACAGAGATTAGTCACTCAGGTGATGATATTCCCATCAAGGGGAAGACTGCCTGTACAAAGTTTACCAGACTCAGTACCAATGGAATCCATAGTCTTGTCTTGTGCGAGCCAGTCACTGGCCGAACTCATCAAATACGTGTCCATTTGCAACATTTAGGCTATCCGATAGCCAATGACGCGCTTTACCTGTCCAAACATGTTAATGGTCGTTCAACTGAAGGAATGGGTGCTGATAGAGCTGCTGCAAATTCCAGCCCCTCTCCGCCATCTGGCTTTCGCGAAAATTTATCCACTGAACCTGTAGATTCCAATGAAGATTTCAGCATTGATCCCATGTGTACAAATTGTCCGAATTTGGTTCCAAAAGGATACGATGTTTATGAAGAGGCTTTGTGGCTGCACTGTGTTCGGTATTCCGCACCGGGATGGACTTATGAATGCCCATATCCTGATTGGGCATCACTTAGCTAA

Protein Analysis

384

Amino Acids

43.35

Weight (kDa)

7.01

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 123 - 278 9.1e-30 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 96, 259, 469
AccB7I CCANNNNNTGG 1 cut(s) 797
AccII CGCG 3 cut(s) 87, 860, 960
AciI CCGC 5 cut(s) 62, 68, 152, 944, 1098
AclWI GGATC 1 cut(s) 1004
AcoI YGGCCR 3 cut(s) 59, 525, 799
AcsI RAATTY 4 cut(s) 614, 928, 964, 1033
AcuI CTGAAG 1 cut(s) 918
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 4 cut(s) 593, 736, 757, 1021
AfiI CCNNNNNNNGG 5 cut(s) 523, 719, 720, 797, 950
AflIII ACRYGT 2 cut(s) 817, 877
AgsI TTSAA 5 cut(s) 190, 356, 540, 569, 894
AjiI CACGTC 1 cut(s) 12
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
AleI CACNNNNGTG 1 cut(s) 699
AluBI AGCT 2 cut(s) 920, 1149
AluI AGCT 2 cut(s) 920, 1149
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 1 cut(s) 1004
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 663
AoxI GGCC 5 cut(s) 59, 193, 197, 525, 799
ApeKI GCWGC 4 cut(s) 559, 920, 923, 1078
ApoI RAATTY 4 cut(s) 614, 928, 964, 1033
Asp700I GAANNNNTTC 1 cut(s) 535
AspLEI GCGC 2 cut(s) 99, 862
AspS9I GGNCC 1 cut(s) 580
AsuC2I CCSGG 1 cut(s) 1104
AsuHPI GGTGA 1 cut(s) 713
AvaI CYCGRG 1 cut(s) 663
AvaII GGWCC 1 cut(s) 580
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 3 cut(s) 96, 259, 469
BbsI GAAGAC 3 cut(s) 24, 164, 731
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 4 cut(s) 571, 907, 910, 1065
BccI CCATC 7 cut(s) 22, 170, 242, 250, 722, 955, 1101
BcgI CGANNNNNNTGC 2 cut(s) 1070, 1104
BciVI GTATCC 2 cut(s) 231, 1043
BcnI CCSGG 1 cut(s) 1104
BcoDI GTCTC 1 cut(s) 511
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 981
BfoI RGCGCY 1 cut(s) 100
BfuI GTATCC 2 cut(s) 231, 1043
BisI GCNGC 5 cut(s) 62, 560, 921, 924, 1079
BlpI GCTNAGC 1 cut(s) 456
BlsI GCNGC 5 cut(s) 63, 561, 922, 925, 1080
Bme1390I CCNGG 1 cut(s) 1104
Bme18I GGWCC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 663
BmgBI CACGTC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 4 cut(s) 98, 261, 471, 1041
BmrFI CCNGG 1 cut(s) 1104
BmrI ACTGGG 1 cut(s) 320
BmsI GCATC 6 cut(s) 401, 415, 453, 631, 649, 1148
BmuI ACTGGG 1 cut(s) 320
BpiI GAAGAC 3 cut(s) 24, 164, 731
Bpu10I CCTNAGC 1 cut(s) 621
Bpu1102I GCTNAGC 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 1104
Bsa29I ATCGAT 1 cut(s) 347
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 97
BsaWI WCCGGW 2 cut(s) 364, 400
BsaXI ACNNNNNCTCC 6 cut(s) 23, 53, 243, 273, 564, 594
Bsc4I CCNNNNNNNGG 5 cut(s) 523, 719, 720, 797, 950
Bse118I RCCGGY 2 cut(s) 195, 262
Bse1I ACTGG 3 cut(s) 326, 791, 802
Bse3DI GCAATG 1 cut(s) 594
Bse8I GATNNNNATC 1 cut(s) 32
BseCI ATCGAT 1 cut(s) 347
BseGI GGATG 4 cut(s) 373, 406, 444, 1112
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 5 cut(s) 523, 719, 720, 797, 950
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 5 cut(s) 447, 522, 612, 711, 766
BseNI ACTGG 3 cut(s) 326, 791, 802
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 4 cut(s) 571, 907, 910, 1065
BseYI CCCAGC 1 cut(s) 608
BsgI GTGCAG 2 cut(s) 240, 1064
Bsh1236I CGCG 3 cut(s) 87, 860, 960
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 5 cut(s) 61, 195, 199, 527, 801
BshNI GGYRCC 3 cut(s) 96, 259, 469
BshVI ATCGAT 1 cut(s) 347
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 5 cut(s) 196, 263, 365, 401, 1103
BslI CCNNNNNNNGG 5 cut(s) 523, 719, 720, 797, 950
BsmAI GTCTC 1 cut(s) 511
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 5 cut(s) 61, 195, 199, 527, 801
BsoBI CYCGRG 1 cut(s) 663
Bsp1286I GDGCHC 1 cut(s) 680
Bsp1407I TGTACA 2 cut(s) 734, 1019
Bsp143I GATC 3 cut(s) 33, 496, 1009
Bsp1720I GCTNAGC 1 cut(s) 456
Bsp68I TCGCGA 2 cut(s) 87, 960
BspACI CCGC 5 cut(s) 62, 68, 152, 944, 1098
BspANI GGCC 5 cut(s) 61, 195, 199, 527, 801
BspCNI CTCAG 5 cut(s) 448, 521, 613, 710, 765
BspDI ATCGAT 1 cut(s) 347
BspFNI CGCG 3 cut(s) 87, 860, 960
BspHI TCATGA 3 cut(s) 181, 330, 655
BspLI GGNNCC 4 cut(s) 98, 261, 471, 1041
BspPI GGATC 1 cut(s) 1004
BspT107I GGYRCC 3 cut(s) 96, 259, 469
BsrDI GCAATG 1 cut(s) 594
BsrFI RCCGGY 2 cut(s) 195, 262
BsrGI TGTACA 2 cut(s) 734, 1019
BsrI ACTGG 3 cut(s) 326, 791, 802
BssAI RCCGGY 2 cut(s) 195, 262
BssMI GATC 3 cut(s) 33, 496, 1009
BssNI GRCGYC 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 436, 591, 1085
Bst6I CTCTTC 1 cut(s) 1059
BstACI GRCGYC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 605
BstAUI TGTACA 2 cut(s) 734, 1019
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 3 cut(s) 197, 390, 788
BstDEI CTNAG 6 cut(s) 456, 508, 621, 697, 752, 1145
BstF5I GGATG 4 cut(s) 373, 406, 444, 1112
BstFNI CGCG 3 cut(s) 87, 860, 960
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 2 cut(s) 99, 862
BstKTI GATC 3 cut(s) 36, 499, 1012
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 3 cut(s) 33, 496, 1009
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 6 cut(s) 67, 533, 542, 605, 857, 917
BstNSI RCATGY 1 cut(s) 881
BstSCI CCNGG 1 cut(s) 1102
BstSFI CTRYAG 1 cut(s) 981
BstUI CGCG 3 cut(s) 87, 860, 960
BstV1I GCAGC 4 cut(s) 571, 907, 910, 1065
BstV2I GAAGAC 3 cut(s) 24, 164, 731
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 347
BsuI GTATCC 2 cut(s) 231, 1043
BsuRI GGCC 5 cut(s) 61, 195, 199, 527, 801
BsuTUI ATCGAT 1 cut(s) 347
BtgZI GCGATG 1 cut(s) 41
BtrI CACGTC 1 cut(s) 12
BtsCI GGATG 4 cut(s) 373, 406, 444, 1112
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 4 cut(s) 228, 795, 972, 1081
BtuMI TCGCGA 2 cut(s) 87, 960
Cac8I GCNNGC 3 cut(s) 197, 390, 788
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 3 cut(s) 181, 330, 655
CfoI GCGC 2 cut(s) 99, 862
Cfr10I RCCGGY 2 cut(s) 195, 262
Cfr13I GGNCC 1 cut(s) 580
ClaI ATCGAT 1 cut(s) 347
CseI GACGC 1 cut(s) 866
Csp6I GTAC 4 cut(s) 592, 735, 756, 1020
CviAII CATG 9 cut(s) 101, 134, 182, 317, 331, 461, 656, 878, 1015
CviQI GTAC 4 cut(s) 592, 735, 756, 1020
DdeI CTNAG 6 cut(s) 456, 508, 621, 697, 752, 1145
DinI GGCGCC 1 cut(s) 98
DpnI GATC 3 cut(s) 35, 498, 1011
DpnII GATC 3 cut(s) 33, 496, 1009
EaeI YGGCCR 3 cut(s) 59, 525, 799
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 1059
EarI CTCTTC 1 cut(s) 1059
EciI GGCGGA 1 cut(s) 933
EclXI CGGCCG 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 580
Eco52I CGGCCG 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 918
Eco88I CYCGRG 1 cut(s) 663
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
FaeI CATG 9 cut(s) 104, 137, 185, 320, 334, 464, 659, 881, 1018
FatI CATG 9 cut(s) 100, 133, 181, 316, 330, 460, 655, 877, 1014
FauI CCCGC 1 cut(s) 145
Fnu4HI GCNGC 5 cut(s) 62, 560, 921, 924, 1079
FokI GGATG 4 cut(s) 380, 393, 431, 1119
FseI GGCCGGCC 1 cut(s) 199
Fsp4HI GCNGC 5 cut(s) 62, 560, 921, 924, 1079
FspBI CTAG 1 cut(s) 494
GlaI GCGC 2 cut(s) 98, 861
GluI GCNGC 5 cut(s) 62, 560, 921, 924, 1079
GsaI CCCAGC 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 5 cut(s) 61, 195, 199, 527, 801
HapII CCGG 5 cut(s) 196, 263, 365, 401, 1103
HgaI GACGC 1 cut(s) 866
HhaI GCGC 2 cut(s) 99, 862
Hin1I GRCGYC 1 cut(s) 97
Hin1II CATG 9 cut(s) 104, 137, 185, 320, 334, 464, 659, 881, 1018
Hin6I GCGC 2 cut(s) 97, 860
HinP1I GCGC 2 cut(s) 97, 860
HinfI GANTC 8 cut(s) 46, 55, 142, 349, 513, 750, 765, 986
HpaII CCGG 5 cut(s) 196, 263, 365, 401, 1103
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 4 cut(s) 177, 310, 744, 1020
Hpy188I TCNGA 3 cut(s) 107, 846, 1032
Hpy8I GTNNAC 4 cut(s) 177, 310, 744, 1020
Hpy99I CGWCG 2 cut(s) 92, 289
HpyAV CCTTC 3 cut(s) 228, 563, 893
HpyCH4III ACNGT 3 cut(s) 436, 591, 1085
HpyCH4IV ACGT 3 cut(s) 11, 284, 817
HpyCH4V TGCA 7 cut(s) 221, 392, 406, 599, 829, 926, 1081
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 533, 542, 605, 857, 917
HpyF3I CTNAG 6 cut(s) 456, 508, 621, 697, 752, 1145
HpySE526I ACGT 3 cut(s) 11, 284, 817
Hsp92I GRCGYC 1 cut(s) 97
Hsp92II CATG 9 cut(s) 104, 137, 185, 320, 334, 464, 659, 881, 1018
HspAI GCGC 2 cut(s) 97, 860
KasI GGCGCC 1 cut(s) 96
KroI GCCGGC 1 cut(s) 195
KroNI GCCGGC 1 cut(s) 197
Kzo9I GATC 3 cut(s) 33, 496, 1009
LmnI GCTCC 1 cut(s) 675
Lsp1109I GCAGC 4 cut(s) 571, 907, 910, 1065
LweI GCATC 6 cut(s) 401, 415, 453, 631, 649, 1148
MaeI CTAG 1 cut(s) 494
MaeII ACGT 3 cut(s) 11, 284, 817
MaeIII GTNAC 2 cut(s) 692, 793
MalI GATC 3 cut(s) 35, 498, 1011
MboI GATC 3 cut(s) 33, 496, 1009
MboII GAAGA 8 cut(s) 16, 29, 151, 169, 344, 736, 1007, 1076
MhlI GDGCHC 1 cut(s) 680
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 6 cut(s) 483, 614, 928, 964, 1024, 1033
MluNI TGGCCA 1 cut(s) 527
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 3 cut(s) 55, 507, 744
MnlI CCTC 6 cut(s) 87, 445, 483, 566, 949, 1060
Mox20I TGGCCA 1 cut(s) 527
MroNI GCCGGC 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 535
MscI TGGCCA 1 cut(s) 527
MseI TTAA 3 cut(s) 279, 651, 882
MslI CAYNNNNRTG 5 cut(s) 315, 411, 699, 882, 1105
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 5 cut(s) 196, 263, 365, 401, 1103
MspR9I CCNGG 1 cut(s) 1104
Mva1269I GAATGC 1 cut(s) 1124
MvnI CGCG 3 cut(s) 87, 860, 960
MwoI GCNNNNNNNGC 6 cut(s) 67, 533, 542, 605, 857, 917
NaeI GCCGGC 1 cut(s) 197
NarI GGCGCC 1 cut(s) 97
NciI CCSGG 1 cut(s) 1104
NdeII GATC 3 cut(s) 33, 496, 1009
NgoMIV GCCGGC 1 cut(s) 195
NlaIII CATG 9 cut(s) 104, 137, 185, 320, 334, 464, 659, 881, 1018
NlaIV GGNNCC 4 cut(s) 98, 261, 471, 1041
NmuCI GTSAC 2 cut(s) 692, 793
NruI TCGCGA 2 cut(s) 87, 960
NspI RCATGY 1 cut(s) 881
OliI CACNNNNGTG 1 cut(s) 699
PaeR7I CTCGAG 1 cut(s) 663
PagI TCATGA 3 cut(s) 181, 330, 655
PciI ACATGT 1 cut(s) 877
PctI GAATGC 1 cut(s) 1124
PdiI GCCGGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 5 cut(s) 55, 142, 349, 765, 986
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 5 cut(s) 63, 561, 922, 925, 1080
PleI GAGTC 3 cut(s) 54, 507, 744
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 3 cut(s) 54, 507, 744
Ppu21I YACGTR 1 cut(s) 818
PscI ACATGT 1 cut(s) 877
PspFI CCCAGC 1 cut(s) 608
PspN4I GGNNCC 4 cut(s) 98, 261, 471, 1041
PspPI GGNCC 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 797
RigI GGCCGGCC 1 cut(s) 199
RruI TCGCGA 2 cut(s) 87, 960
RsaI GTAC 4 cut(s) 593, 736, 757, 1021
RsaNI GTAC 4 cut(s) 592, 735, 756, 1020
RseI CAYNNNNRTG 5 cut(s) 315, 411, 699, 882, 1105
SaqAI TTAA 3 cut(s) 279, 651, 882
SatI GCNGC 5 cut(s) 62, 560, 921, 924, 1079
Sau3AI GATC 3 cut(s) 33, 496, 1009
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 3 cut(s) 55, 507, 744
ScrFI CCNGG 1 cut(s) 1104
SduI GDGCHC 1 cut(s) 680
SfaNI GCATC 6 cut(s) 401, 415, 453, 631, 649, 1148
SfcI CTRYAG 1 cut(s) 981
SfoI GGCGCC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 580
SlaI CTCGAG 1 cut(s) 663
SmiMI CAYNNNNRTG 5 cut(s) 315, 411, 699, 882, 1105
SmlI CTYRAG 2 cut(s) 449, 663
SmoI CTYRAG 2 cut(s) 449, 663
Sse9I AATT 6 cut(s) 483, 614, 928, 964, 1024, 1033
SsiI CCGC 5 cut(s) 62, 68, 152, 944, 1098
SspDI GGCGCC 1 cut(s) 96
SspMI CTAG 1 cut(s) 494
StyD4I CCNGG 1 cut(s) 1102
TaaI ACNGT 3 cut(s) 436, 591, 1085
TaiI ACGT 3 cut(s) 14, 287, 820
TaqI TCGA 4 cut(s) 120, 347, 490, 664
TasI AATT 6 cut(s) 483, 614, 928, 964, 1024, 1033
TatI WGTACW 3 cut(s) 591, 734, 1019
TauI GCSGC 1 cut(s) 64
TfiI GAWTC 5 cut(s) 55, 142, 349, 765, 986
Tru1I TTAA 3 cut(s) 279, 651, 882
Tru9I TTAA 3 cut(s) 279, 651, 882
TscAI CASTG 4 cut(s) 228, 802, 979, 1088
TseFI GTSAC 2 cut(s) 692, 793
TseI GCWGC 4 cut(s) 559, 920, 923, 1078
Tsp45I GTSAC 2 cut(s) 692, 793
TspDTI ATGAA 7 cut(s) 17, 115, 333, 476, 1008, 1077, 1131
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 4 cut(s) 228, 802, 979, 1088
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 4 cut(s) 614, 928, 964, 1033
XceI RCATGY 1 cut(s) 881
XcmI CCANNNNNNNNNTGG 2 cut(s) 333, 1131
XhoI CTCGAG 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.