pycom14g13000

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016821
Physical Location & Seq
Forward (+)
16036415 .. 16036738
324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g13000.1

Sequence Viewer

Length: 324 bp
ATGAAGGCCGACGAGGACGTCCGTATGATCTCCGCCGAGGCCCCCGTACTCTTCGCCAAGGCCTGCGAGCTCTTCATCCTCGAGCTCACCATCCGCTCATGGCTCCACGCCGAGGAGAACAAGCGCCGGACTCTCCAGAAAAACGACATTGCCGCCGCCATTACCCGCACCGATATATTCGATTTCTTGGTGGATATTGTGCCCCGCGACGATCGATCAAGGAGGAGGCGGTGGGTCTCGGAGGTATGGTCGGGGCGACGGCGAGTGGGATGCCGTATTTCTATCCGCCGATGGGTCAGCCGGCTGGTGGCGCCGGGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0005737 GO:0005829 GO:0006109 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010029 GO:0010033 GO:0010099 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010675 GO:0010677 GO:0016602 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0032870 GO:0032881 GO:0032991 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044798 GO:0045893 GO:0045912 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090575 GO:0097305 GO:0097306 GO:0140110 GO:1900140 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000028 GO:2000030 GO:2000112 GO:2000241 GO:2000306 GO:2000904 GO:2000905 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 map04612 map05152
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

108

Amino Acids

12.52

Weight (kDa)

10.65

Isoelectric Point (pI)

93.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 1 - 54 1e-10 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Histone PF00125 3 - 54 2.4e-12 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016821)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g11310
malus_domestica MD17G1250700.v1.1
prunus_persica Prupe.3G149300_v2.0.a1
pyrus_communis pycom09g17270 pycom14g13000 pycom17g25460
rosa_chinensis RchiOBHm_Chr4g0407551
rosa_laevigata RLG00000008711
rosa_roxburghii Rroxscaffold_5G00352170
rosa_rugosa Rorug04G0080300
rosa_samantha Rh4AG145000 Rh4BG142900 Rh4CG151700 Rh4DG138900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
AatII GACGTC 1 cut(s) 21
AccB1I GGYRCC 1 cut(s) 310
AccBSI CCGCTC 1 cut(s) 96
AccII CGCG 1 cut(s) 207
AciI CCGC 8 cut(s) 33, 94, 153, 156, 166, 205, 229, 286
AcyI GRCGYC 2 cut(s) 18, 311
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 3 cut(s) 112, 292, 307
AluBI AGCT 2 cut(s) 70, 85
AluI AGCT 2 cut(s) 70, 85
Alw21I GWGCWC 2 cut(s) 72, 87
Alw26I GTCTC 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 3 cut(s) 6, 39, 60
AspLEI GCGC 2 cut(s) 126, 313
AspS9I GGNCC 1 cut(s) 40
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 2 cut(s) 72, 87
Bbv12I GWGCWC 2 cut(s) 72, 87
BccI CCATC 2 cut(s) 98, 285
BceAI ACGGC 2 cut(s) 258, 275
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 241
BfoI RGCGCY 2 cut(s) 127, 314
BisI GCNGC 2 cut(s) 153, 156
BlsI GCNGC 2 cut(s) 154, 157
Bme1390I CCNGG 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 40
BmiI GGNNCC 3 cut(s) 42, 104, 312
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 315
Bsa29I ATCGAT 1 cut(s) 214
BsaHI GRCGYC 2 cut(s) 18, 311
BsaI GGTCTC 1 cut(s) 241
BsaJI CCNNGG 4 cut(s) 36, 57, 111, 314
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 292, 307
Bse118I RCCGGY 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 147
BseCI ATCGAT 1 cut(s) 214
BseDI CCNNGG 4 cut(s) 36, 57, 111, 314
BseGI GGATG 3 cut(s) 75, 90, 275
BseLI CCNNNNNNNGG 3 cut(s) 112, 292, 307
BseMI GCAATG 1 cut(s) 147
BseRI GAGGAG 2 cut(s) 128, 238
BseSI GKGCMC 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 3 cut(s) 8, 41, 62
BshNI GGYRCC 1 cut(s) 310
BshVI ATCGAT 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 2 cut(s) 72, 87
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 3 cut(s) 127, 301, 314
BslI CCNNNNNNNGG 3 cut(s) 112, 292, 307
BsmAI GTCTC 1 cut(s) 241
BsnI GGCC 3 cut(s) 8, 41, 62
Bso31I GGTCTC 1 cut(s) 241
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 72, 87, 204
Bsp143I GATC 3 cut(s) 27, 211, 215
BspACI CCGC 8 cut(s) 33, 94, 153, 156, 166, 205, 229, 286
BspANI GGCC 3 cut(s) 8, 41, 62
BspDI ATCGAT 1 cut(s) 214
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 42, 104, 312
BspQI GCTCTTC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 241
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 300
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 4 cut(s) 36, 57, 111, 314
BssMI GATC 3 cut(s) 27, 211, 215
BssNI GRCGYC 2 cut(s) 18, 311
BssT1I CCWWGG 1 cut(s) 57
Bst6I CTCTTC 2 cut(s) 56, 77
BstACI GRCGYC 2 cut(s) 18, 311
BstC8I GCNNGC 3 cut(s) 64, 68, 302
BstF5I GGATG 3 cut(s) 75, 90, 275
BstFNI CGCG 1 cut(s) 207
BstH2I RGCGCY 2 cut(s) 127, 314
BstHHI GCGC 2 cut(s) 126, 313
BstKTI GATC 3 cut(s) 30, 214, 218
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 3 cut(s) 27, 211, 215
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstSCI CCNGG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 204
BstUI CGCG 1 cut(s) 207
Bsu15I ATCGAT 1 cut(s) 214
BsuRI GGCC 3 cut(s) 8, 41, 62
BsuTUI ATCGAT 1 cut(s) 214
BtsCI GGATG 3 cut(s) 75, 90, 275
Cac8I GCNNGC 3 cut(s) 64, 68, 302
CfoI GCGC 2 cut(s) 126, 313
Cfr10I RCCGGY 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 214
Csp6I GTAC 1 cut(s) 47
CviAII CATG 1 cut(s) 99
CviJI RGCY 8 cut(s) 8, 41, 62, 70, 85, 103, 300, 304
CviKI_1 RGCY 8 cut(s) 8, 41, 62, 70, 85, 103, 300, 304
CviQI GTAC 1 cut(s) 47
DinI GGCGCC 1 cut(s) 312
DpnI GATC 3 cut(s) 29, 213, 217
DpnII GATC 3 cut(s) 27, 211, 215
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 2 cut(s) 56, 77
EarI CTCTTC 2 cut(s) 56, 77
EciI GGCGGA 2 cut(s) 22, 275
Ecl136II GAGCTC 2 cut(s) 70, 85
Eco130I CCWWGG 1 cut(s) 57
Eco147I AGGCCT 1 cut(s) 62
Eco24I GRGCYC 2 cut(s) 72, 87
Eco31I GGTCTC 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 70, 85
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 2 cut(s) 70, 85
EcoO109I RGGNCCY 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 2 cut(s) 72, 87
EgeI GGCGCC 1 cut(s) 312
EheI GGCGCC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 1 cut(s) 102
FaiI YATR 4 cut(s) 26, 100, 176, 247
FatI CATG 1 cut(s) 98
FauI CCCGC 2 cut(s) 173, 212
Fnu4HI GCNGC 2 cut(s) 153, 156
FokI GGATG 3 cut(s) 62, 77, 282
FriOI GRGCYC 2 cut(s) 72, 87
Fsp4HI GCNGC 2 cut(s) 153, 156
GlaI GCGC 2 cut(s) 125, 312
GluI GCNGC 2 cut(s) 153, 156
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 2 cut(s) 127, 314
HaeIII GGCC 3 cut(s) 8, 41, 62
HapII CCGG 3 cut(s) 127, 301, 314
HhaI GCGC 2 cut(s) 126, 313
Hin1I GRCGYC 2 cut(s) 18, 311
Hin1II CATG 1 cut(s) 102
Hin6I GCGC 2 cut(s) 124, 311
HinP1I GCGC 2 cut(s) 124, 311
HinfI GANTC 1 cut(s) 130
HpaII CCGG 3 cut(s) 127, 301, 314
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 1 cut(s) 136
Hpy99I CGWCG 3 cut(s) 14, 212, 261
HpyCH4IV ACGT 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 2 cut(s) 18, 311
Hsp92II CATG 1 cut(s) 102
HspAI GCGC 2 cut(s) 124, 311
KasI GGCGCC 1 cut(s) 310
KroI GCCGGC 1 cut(s) 300
KroNI GCCGGC 1 cut(s) 302
Kzo9I GATC 3 cut(s) 27, 211, 215
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 108
LpnPI CCDG 5 cut(s) 76, 140, 149, 290, 314
LweI GCATC 1 cut(s) 260
MaeII ACGT 1 cut(s) 18
MalI GATC 3 cut(s) 29, 213, 217
MbiI CCGCTC 1 cut(s) 96
MboI GATC 3 cut(s) 27, 211, 215
MboII GAAGA 2 cut(s) 43, 64
MhlI GDGCHC 3 cut(s) 72, 87, 204
Mly113I GGCGCC 1 cut(s) 311
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 7 cut(s) 7, 31, 89, 106, 216, 219, 235
MroNI GCCGGC 1 cut(s) 300
MspI CCGG 3 cut(s) 127, 301, 314
MspR9I CCNGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 310
NaeI GCCGGC 1 cut(s) 302
NarI GGCGCC 1 cut(s) 311
NciI CCSGG 1 cut(s) 315
NdeII GATC 3 cut(s) 27, 211, 215
NgoMIV GCCGGC 1 cut(s) 300
NlaIII CATG 1 cut(s) 102
NlaIV GGNNCC 3 cut(s) 42, 104, 312
NmeAIII GCCGAG 2 cut(s) 61, 136
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 77
PdiI GCCGGC 1 cut(s) 302
PkrI GCNGC 2 cut(s) 154, 157
Ple19I CGATCG 1 cut(s) 214
PleI GAGTC 1 cut(s) 124
PluTI GGCGCC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 2 cut(s) 72, 87
PspN4I GGNNCC 3 cut(s) 42, 104, 312
PspPI GGNCC 1 cut(s) 40
PspXI VCTCGAGB 1 cut(s) 80
PvuI CGATCG 1 cut(s) 214
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SacI GAGCTC 2 cut(s) 72, 87
SapI GCTCTTC 1 cut(s) 77
SatI GCNGC 2 cut(s) 153, 156
Sau3AI GATC 3 cut(s) 27, 211, 215
Sau96I GGNCC 1 cut(s) 40
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 3 cut(s) 72, 87, 204
SetI ASST 4 cut(s) 21, 72, 87, 246
SfaNI GCATC 1 cut(s) 260
SfoI GGCGCC 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SseBI AGGCCT 1 cut(s) 62
SsiI CCGC 8 cut(s) 33, 94, 153, 156, 166, 205, 229, 286
SspDI GGCGCC 1 cut(s) 310
SstI GAGCTC 2 cut(s) 72, 87
StuI AGGCCT 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 57
TaiI ACGT 1 cut(s) 21
TaqI TCGA 3 cut(s) 81, 180, 214
TauI GCSGC 2 cut(s) 155, 158
TspDTI ATGAA 2 cut(s) 17, 64
TspGWI ACGGA 1 cut(s) 11
XhoI CTCGAG 1 cut(s) 80
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.