pycom14g13670

Kelch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006640
Physical Location & Seq
Reverse (-)
16520973 .. 16522861
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGGATACACAAGATTCTGATTTGCCAGAGGTAGTGAAGCCCTGTGGACGTATCAATTCTTGCATGGTTGTTGGAAGAGATACATTATATGTTTACGGGGGAATGATGGAGGTTAAAGATCGAGAAATTACACTCAATGATTTGTATTCTCTAAATCTTAGCAAACTTGACGAGTGGAAGTGCATCATACCGGCAACAGAAACTGAATGGGTGGAGGGTTCCAAGGACGAAGATGAGGATGAAGATGACGATGATGATAGTGAAGATGAAGGTGATGAAGATAGTAACAGTGATGAGACCGATGATGATAATGACGAAGAGAGTGGGAAAGATGGATCGATTCAGATGGGGGATGCTGTTGCTTTAATCAAGGGTGAGGGAAAGAGTCTCCGTAGGAAAGAGAAACGGGCCAGAATTGAGCAGATCAGAGCCAGTCTTGGCCTTTCAGATTCGCAAAGAACCCCAACGCCTGGTGAAACTTTGAGAGAATTCTACAAACGTACAAATATGTACTGGCAAATGGCGGCACACGAACATACTCAACACACTGGAAAGGAGCTCCGTAAGGACGGTTTTGATCTTGCTGAAGCACGATACAGAGAGCTTAAACCAATTCTCGATGAGTTGGCCATATTAGAGGCAGAACAGAAGGCTGAACAAAAAAAAAGGCAAGAAGAAAAGATAAGCGGTTCGCGATGGTGCACACAGTACTGCTATATCGAGTGTATTTTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4110 Kelch_3 Kelch_4 Kelch_5

Protein Analysis

246

Amino Acids

28.18

Weight (kDa)

4.49

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4110 PF13422 147 - 227 2e-29 Domain of unknown function (DUF4110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 470
AccII CGCG 1 cut(s) 694
AciI CCGC 2 cut(s) 524, 687
AclWI GGATC 1 cut(s) 343
AcoI YGGCCR 1 cut(s) 627
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 606
AfaI GTAC 3 cut(s) 502, 512, 710
AfiI CCNNNNNNNGG 1 cut(s) 470
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 2 cut(s) 559, 604
AluI AGCT 2 cut(s) 559, 604
Alw21I GWGCWC 2 cut(s) 561, 704
Alw26I GTCTC 2 cut(s) 290, 392
Alw44I GTGCAC 1 cut(s) 700
AlwI GGATC 1 cut(s) 343
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 3 cut(s) 408, 439, 627
ApaLI GTGCAC 1 cut(s) 700
ApoI RAATTY 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 408
AsuHPI GGTGA 3 cut(s) 284, 386, 485
BaeGI GKGCMC 1 cut(s) 704
BalI TGGCCA 1 cut(s) 629
BanII GRGCYC 1 cut(s) 561
Bbv12I GWGCWC 2 cut(s) 561, 704
BccI CCATC 4 cut(s) 100, 326, 340, 690
BciT130I CCWGG 1 cut(s) 471
BcoDI GTCTC 2 cut(s) 290, 392
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
BmcAI AGTACT 1 cut(s) 710
Bme1390I CCNGG 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 408
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 471
BmsI GCATC 2 cut(s) 192, 343
Bsa29I ATCGAT 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 3 cut(s) 432, 518, 553
BseBI CCWGG 1 cut(s) 471
BseCI ATCGAT 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 2 cut(s) 244, 358
BseLI CCNNNNNNNGG 1 cut(s) 470
BseNI ACTGG 3 cut(s) 432, 518, 553
BseSI GKGCMC 1 cut(s) 704
Bsh1236I CGCG 1 cut(s) 694
BshFI GGCC 3 cut(s) 410, 441, 629
BshVI ATCGAT 1 cut(s) 338
BsiHKAI GWGCWC 2 cut(s) 561, 704
BsiSI CCGG 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 290, 392
BsnI GGCC 3 cut(s) 410, 441, 629
Bso31I GGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 561, 704
Bsp143I GATC 4 cut(s) 118, 335, 423, 577
Bsp68I TCGCGA 1 cut(s) 694
BspACI CCGC 2 cut(s) 524, 687
BspANI GGCC 3 cut(s) 410, 441, 629
BspDI ATCGAT 1 cut(s) 338
BspFNI CGCG 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 220
BspPI GGATC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 290
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 3 cut(s) 432, 518, 553
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 4 cut(s) 118, 335, 423, 577
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 3 cut(s) 290, 572, 708
Bst6I CTCTTC 2 cut(s) 70, 312
BstDEI CTNAG 1 cut(s) 158
BstF5I GGATG 2 cut(s) 244, 358
BstFNI CGCG 1 cut(s) 694
BstKTI GATC 4 cut(s) 121, 338, 426, 580
BstMAI GTCTC 2 cut(s) 290, 392
BstMBI GATC 4 cut(s) 118, 335, 423, 577
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 704
BstUI CGCG 1 cut(s) 694
Bsu15I ATCGAT 1 cut(s) 338
BsuRI GGCC 3 cut(s) 410, 441, 629
BsuTUI ATCGAT 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 709
BtsCI GGATG 2 cut(s) 244, 358
BtsIMutI CAGTG 2 cut(s) 295, 546
BtuMI TCGCGA 1 cut(s) 694
CaiI CAGNNNCTG 1 cut(s) 203
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 408
ClaI ATCGAT 1 cut(s) 338
Csp6I GTAC 3 cut(s) 501, 511, 709
CviAII CATG 1 cut(s) 64
CviJI RGCY 8 cut(s) 40, 410, 431, 441, 559, 604, 629, 653
CviKI_1 RGCY 8 cut(s) 40, 410, 431, 441, 559, 604, 629, 653
CviQI GTAC 3 cut(s) 501, 511, 709
DdeI CTNAG 1 cut(s) 158
DpnI GATC 4 cut(s) 120, 337, 425, 579
DpnII GATC 4 cut(s) 118, 335, 423, 577
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 2 cut(s) 70, 312
EarI CTCTTC 2 cut(s) 70, 312
Ecl136II GAGCTC 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 561
Eco31I GGTCTC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 559
Eco57I CTGAAG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 67
FaiI YATR 8 cut(s) 65, 88, 90, 188, 509, 537, 632, 717
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 2 cut(s) 251, 365
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 525
GluI GCNGC 1 cut(s) 525
HaeIII GGCC 3 cut(s) 410, 441, 629
HapII CCGG 1 cut(s) 191
Hin1II CATG 1 cut(s) 67
HinfI GANTC 4 cut(s) 14, 340, 385, 449
HpaII CCGG 1 cut(s) 191
HphI GGTGA 3 cut(s) 284, 386, 485
Hpy166II GTNNAC 3 cut(s) 47, 94, 702
Hpy188I TCNGA 4 cut(s) 19, 345, 428, 448
Hpy188III TCNNGA 3 cut(s) 122, 617, 693
Hpy8I GTNNAC 3 cut(s) 47, 94, 702
HpyAV CCTTC 2 cut(s) 263, 643
HpyCH4III ACNGT 3 cut(s) 290, 572, 708
HpyCH4IV ACGT 2 cut(s) 49, 499
HpyCH4V TGCA 3 cut(s) 63, 183, 702
HpyF3I CTNAG 1 cut(s) 158
HpySE526I ACGT 2 cut(s) 49, 499
Hsp92II CATG 1 cut(s) 67
Kzo9I GATC 4 cut(s) 118, 335, 423, 577
LmnI GCTCC 2 cut(s) 556, 564
LpnPI CCDG 9 cut(s) 39, 55, 204, 424, 445, 456, 483, 499, 534
LweI GCATC 2 cut(s) 192, 343
MaeII ACGT 2 cut(s) 49, 499
MaeIII GTNAC 1 cut(s) 284
MalI GATC 4 cut(s) 120, 337, 425, 579
MboI GATC 4 cut(s) 118, 335, 423, 577
MboII GAAGA 7 cut(s) 87, 242, 254, 275, 290, 329, 686
MhlI GDGCHC 2 cut(s) 561, 704
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 5 cut(s) 55, 126, 414, 488, 612
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 6 cut(s) 22, 103, 208, 229, 370, 631
Mox20I TGGCCA 1 cut(s) 629
MscI TGGCCA 1 cut(s) 629
MseI TTAA 3 cut(s) 114, 365, 606
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 1 cut(s) 694
NdeII GATC 4 cut(s) 118, 335, 423, 577
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 1 cut(s) 220
NruI TCGCGA 1 cut(s) 694
PfeI GAWTC 3 cut(s) 14, 340, 449
PflMI CCANNNNNTGG 1 cut(s) 470
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 1 cut(s) 408
PstNI CAGNNNCTG 1 cut(s) 203
RruI TCGCGA 1 cut(s) 694
RsaI GTAC 3 cut(s) 502, 512, 710
RsaNI GTAC 3 cut(s) 501, 511, 709
SacI GAGCTC 1 cut(s) 561
SaqAI TTAA 3 cut(s) 114, 365, 606
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 4 cut(s) 118, 335, 423, 577
Sau96I GGNCC 1 cut(s) 408
ScaI AGTACT 1 cut(s) 710
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 2 cut(s) 561, 704
SetI ASST 7 cut(s) 33, 52, 114, 274, 502, 561, 606
SfaNI GCATC 2 cut(s) 192, 343
Sse9I AATT 5 cut(s) 55, 126, 414, 488, 612
SsiI CCGC 2 cut(s) 524, 687
SstI GAGCTC 1 cut(s) 561
StyD4I CCNGG 1 cut(s) 469
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 290, 572, 708
TaiI ACGT 2 cut(s) 52, 502
TaqI TCGA 4 cut(s) 121, 338, 618, 720
TaqII GACCGA 1 cut(s) 314
TasI AATT 5 cut(s) 55, 126, 414, 488, 612
TatI WGTACW 2 cut(s) 510, 708
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 3 cut(s) 14, 340, 449
Tru1I TTAA 3 cut(s) 114, 365, 606
Tru9I TTAA 3 cut(s) 114, 365, 606
TscAI CASTG 2 cut(s) 295, 553
TspDTI ATGAA 3 cut(s) 255, 282, 291
TspGWI ACGGA 2 cut(s) 380, 551
TspRI CASTG 2 cut(s) 295, 553
Van91I CCANNNNNTGG 1 cut(s) 470
VneI GTGCAC 1 cut(s) 700
XapI RAATTY 1 cut(s) 488
ZrmI AGTACT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.