pycom14g14690

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013824
Physical Location & Seq
Reverse (-)
17398764 .. 17402760
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2460 bp
ATGGCGCCTCCGAGTCCATCTGCTCCTGATCGTTCTCAGCCCTCTTCGTCTTCGGACCCGAAGAGCAACAAGAAAGATTTTTCGACTGCTATCTTGGAACGAAAGAAGTCTCCTAATCGACTTGTTGTCGATGAAGCTGTCAATGATGATAATTCTGTGATTTCCTTGCACCCTGCTGCACTGGAGAAGCTTCAGTTTTTCCGCGGAGACACTGTTTTAATTAAGGGCAAGAAAAGGAGAGACACAATTTGCATCATTATTGCCGATGATGAATGCGAGGAATCGAAAATCAGAATGAACAAAGTTGTGAGAGCTAATCTTAGGGTTCGCCTTGGAGATGTTGTGTCTGTTCATCCCTGCCCTGATGTCAAGTATGGAAAGAAGGTTCACATCCTTCCAATCGATGATACAATTGAAGGAGTGACAGGAAGCTTATTCGATGCATATTTGAAGCCATACTTCGTTGAATCTTACCGGCCTATGAGGAAGGGTGATCTTTTCCTGGTTCGGGGCATGATGCGAAGCGTTGAGTTCAAGGTCATGGAGACAGACCCTGCTGAGTACTGCATTGTTGCGCCAGATACTGAGATTTTCTGTGAGGGAGAGCCTGTCAAACGTGAAGACGAGGAAAGACTAGAGGATGTGGGATATGATGATGTTGGAGGTGTTAGAAAGCAAATGGCCCAGATACGCGAGTTAGTAGAACTGCCTCTTAGACATCCGCAGCTCTTCAAATCGATTGGAGTAAAACCCCCTAAAGGCATTTTACTCTATGGCCCACCGGGGACTGGAAAAACTTTGATTGCAAGGGCTGTAGCGAATGAGACTGGAGCGTTCTTCCTTTTGATTAATGGACCTGAAATAATGTCCAAGATGGCAGGTGAAAGTGAAAGTAACTTGAGGAAGGCGTTTGAGGAAGCCGAAAAGAATGCACCCTCAATCATTTTTATTGATGAGATAGATTCTATTGCTCCAAAGAGAGAAAAGACCCACGGTGAAGTGGAAAGGCGTATAGTTTCCCAGCTTCTGACTTTAATGGATGGCCTTAAGTCCCGTGCGCATGTCATTGTCATGGGAGCAACGAATAGGCCAAACAGCATCGACCCTGCTCTGAGGAGATTTGGAAGATTCGATAGAGAGATTGACATTGGTGTGCCGGATGAAGTTGGAAGATTGGAAGTTTTACGAATCCATACAAAGAGGATGAAACTTGCAGACGATGTTGATCTCGAAAGAGTTGCAAAAGATACCCATGGTTTTGTTGGTGCAGATCTTGCTGCTCTCTGCACAGAGGCCGCACTCCAGTGCATCCGCGAAAAAATGGATGTTATTGATTTGGAAGACGAAACGATTGATGCTGAGGTGTTGAATTCGATGGCTGTGACAAATGAACACTTCCAGACCTCGTTGTCCTCTACAAATCCCTCTGCCTTGCGCGAAACTGTTGTGGAGGTACCAAATGTCTCATGGGATGCCATTGGTGGGTTGAACAACGTCAAAAGAGAACTTCAAGAGACTGTTTCAATGCCCGTGGAGCATCCAGAAATGTTTGAGAAATTTGGCATGTCGCCTTCCAAAGGCGTTCTCTTTTATGGACCGCCTGGCTGTGGGAAAACTCTTCTCGCCAAGGCAATTGCCAGCGAATGTCAGGCCAACTTCATCAGTATCAAAGGGCCCGAGTTGCTAACAATGTGGTTCGGAGAAAGTGAGGCAAATGTGCGCGAGATATTTGACAAGGCTCGCCAATCTGCTCCTTGTGTTCTCTTCTTTGATGAGCTAGACTCAATTGCAACTCAGCGTGGAAATTCTGTGGGAGATGCCGGTGGTGCTGGAGACAGAGTCTTGAACCAACTTCTAACTGAAATGGATGGAATGAACGCAAAGAAAACCGTCTTTATCATTGGGGCAACAAACAGGCCAGATATTCTTGACCCGGCATTGCTCAGGCCTGGACGTCTTGACCAACTTATCTACATCCCACTCCCAGATGAGGCTTCGCGCCTTCAGATTTTTAAAGCATGTTTGCGCAAGTCCCCCATCTCTAAGGATATTGACCTAGCAGCTCTTGCCCGTTATACGCACGGTTTTAGTGGTGCAGATATCACTGAGATATGTCAACGTGCCTGCAAATATGCCATCAGAGAAGACATTGAGAAGGATATTGAGAGGGAAAGAAGAAAGAATGCTAACCCCGAAGCCATGGAAGAAGATGAAATTGATGAGGTAGCCGAGATCAAGCCAGCACACTTTGAGGAATCGATGAAATATGCCCGAAGAAGTGTGAGTGATGCAGACATCAAGAAGTACCAGCTATTTGCTCAGACTTTGCAGCAATCTCGTGGGTTTGGATCCGAATTTCGATTTCCTGCTCAACAGACAGAGAACAATGCAGGAGAAGCTGCTCTATCCCCATTTTCTTCGGCCGCAGTTGCTGGAGAGGAAGCTGATCTCTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

820

Amino Acids

90.95

Weight (kDa)

5.19

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 40 - 121 6.8e-22 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 142 - 205 4.1e-11 Cell division protein 48 (CDC48), domain 2
RuvB_N PF05496 254 - 329 5.3e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 255 - 384 2.3e-48 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 407 - 448 2.7e-16 AAA+ lid domain
AAA PF00004 528 - 661 1.4e-46 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 683 - 722 4.5e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013824)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09840 AT5G03340
fragaria_vesca FvH4_5g05770 FvH4_5g05770
malus_domestica MD06G1170300.v1.1 MD14G1175800.v1.1
pyrus_communis pycom06g15170 pycom14g14690
rosa_chinensis RchiOBHm_Chr7g0193051
rosa_laevigata RLG00000004299
rosa_multiflora Rmu_sc0028357.1_g000001
rosa_roxburghii Rroxscaffold_3G00262420
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132400 Rh7CG136400 Rh7DG134300
rosa_wichuraiana Rw7G011310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 869
AasI GACNNNNNNGTC 1 cut(s) 1408
AatII GACGTC 1 cut(s) 1957
Acc16I TGCGCA 2 cut(s) 1059, 2027
Acc36I ACCTGC 1 cut(s) 869
Acc65I GGTACC 1 cut(s) 1453
AccB1I GGYRCC 2 cut(s) 4, 1453
AccII CGCG 6 cut(s) 204, 693, 1314, 1437, 1722, 1999
AciI CCGC 7 cut(s) 202, 204, 722, 1296, 1312, 1598, 2424
AclWI GGATC 2 cut(s) 2343, 2356
AcoI YGGCCR 1 cut(s) 2421
AcsI RAATTY 4 cut(s) 1369, 1556, 1804, 2354
AcuI CTGAAG 2 cut(s) 176, 1988
AcyI GRCGYC 2 cut(s) 5, 1954
AfaI GTAC 3 cut(s) 563, 1455, 2306
AfiI CCNNNNNNNGG 7 cut(s) 508, 758, 788, 1482, 1577, 1607, 1990
AflII CTTAAG 1 cut(s) 1046
AhdI GACNNNNNGTC 1 cut(s) 125
AjnI CCWGG 3 cut(s) 501, 1600, 1948
AleI CACNNNNGTG 1 cut(s) 1303
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
Alw26I GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1468, 1508, 1827
AlwI GGATC 2 cut(s) 2343, 2356
AlwNI CAGNNNCTG 4 cut(s) 554, 584, 1027, 2432
Ama87I CYCGRG 1 cut(s) 1676
ApaI GGGCCC 1 cut(s) 1677
ApeKI GCWGC 6 cut(s) 176, 724, 1277, 2060, 2329, 2399
ApoI RAATTY 4 cut(s) 1369, 1556, 1804, 2354
AseI ATTAAT 1 cut(s) 849
Asp718I GGTACC 1 cut(s) 1453
AspLEI GCGC 7 cut(s) 7, 577, 1060, 1437, 1722, 2001, 2028
AspS9I GGNCC 7 cut(s) 55, 682, 776, 854, 1595, 1673, 1674
AsuC2I CCSGG 2 cut(s) 783, 1934
AsuHPI GGTGA 3 cut(s) 503, 893, 1007
AvaI CYCGRG 1 cut(s) 1676
AvaII GGWCC 3 cut(s) 55, 854, 1595
BaeGI GKGCMC 1 cut(s) 1677
BaeI ACNNNNGTAYC 2 cut(s) 680, 713
BamHI GGATCC 1 cut(s) 2348
BanI GGYRCC 2 cut(s) 4, 1453
BanII GRGCYC 1 cut(s) 1677
BauI CACGAG 1 cut(s) 2337
BbsI GAAGAC 4 cut(s) 42, 627, 1347, 2151
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 6 cut(s) 163, 736, 1264, 2072, 2341, 2386
BccI CCATC 7 cut(s) 25, 868, 1034, 1369, 1862, 2045, 2144
BcgI CGANNNNNNTGC 6 cut(s) 911, 945, 1220, 1254, 2318, 2352
BciT130I CCWGG 3 cut(s) 503, 1602, 1950
BcnI CCSGG 2 cut(s) 783, 1934
BcoDI GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1468, 1508, 1827
BfaI CTAG 3 cut(s) 635, 1778, 2057
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 1046
BfuAI ACCTGC 1 cut(s) 869
BglII AGATCT 1 cut(s) 1270
BisI GCNGC 8 cut(s) 177, 725, 1278, 1296, 2061, 2330, 2400, 2424
BlsI GCNGC 8 cut(s) 178, 726, 1279, 1297, 2062, 2331, 2401, 2425
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 5 cut(s) 503, 783, 1602, 1934, 1950
Bme18I GGWCC 3 cut(s) 55, 854, 1595
BmeRI GACNNNNNGTC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 1676
BmgT120I GGNCC 7 cut(s) 55, 682, 776, 854, 1595, 1673, 1674
BmiI GGNNCC 5 cut(s) 6, 57, 1455, 1675, 2350
BmrFI CCNGG 5 cut(s) 503, 783, 1602, 1934, 1950
BpiI GAAGAC 4 cut(s) 42, 627, 1347, 2151
BplI GAGNNNNNCTC 2 cut(s) 1766, 1798
BpmI CTGGAG 5 cut(s) 203, 849, 1286, 1851, 2454
Bpu10I CCTNAGC 2 cut(s) 1359, 1943
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 2 cut(s) 783, 1934
Bsa29I ATCGAT 3 cut(s) 402, 737, 2258
BsaBI GATNNNNATC 1 cut(s) 1224
BsaHI GRCGYC 2 cut(s) 5, 1954
BsaJI CCNNGG 8 cut(s) 202, 331, 782, 991, 1252, 1530, 1626, 2199
BsaXI ACNNNNNCTCC 8 cut(s) 198, 228, 327, 357, 594, 624, 1824, 1854
Bsc4I CCNNNNNNNGG 7 cut(s) 508, 758, 788, 1482, 1577, 1607, 1990
Bse118I RCCGGY 2 cut(s) 474, 1820
Bse1I ACTGG 4 cut(s) 186, 793, 832, 1303
Bse3DI GCAATG 1 cut(s) 1937
Bse8I GATNNNNATC 1 cut(s) 1224
BseBI CCWGG 3 cut(s) 503, 1602, 1950
BseCI ATCGAT 3 cut(s) 402, 737, 2258
BseDI CCNNGG 8 cut(s) 202, 331, 782, 991, 1252, 1530, 1626, 2199
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 7 cut(s) 508, 758, 788, 1482, 1577, 1607, 1990
BseMI GCAATG 1 cut(s) 1937
BseMII CTCAG 9 cut(s) 50, 549, 576, 1103, 1350, 1808, 1957, 2097, 2333
BseNI ACTGG 4 cut(s) 186, 793, 832, 1303
BseRI GAGGAG 1 cut(s) 1129
BseSI GKGCMC 1 cut(s) 1677
BseX3I CGGCCG 1 cut(s) 2421
BseXI GCAGC 6 cut(s) 163, 736, 1264, 2072, 2341, 2386
BseYI CCCAGC 1 cut(s) 1020
BsgI GTGCAG 4 cut(s) 162, 1270, 1287, 2115
Bsh1236I CGCG 6 cut(s) 204, 693, 1314, 1437, 1722, 1999
Bsh1285I CGRYCG 1 cut(s) 2424
BshNI GGYRCC 2 cut(s) 4, 1453
BshVI ATCGAT 3 cut(s) 402, 737, 2258
BsiEI CGRYCG 1 cut(s) 2424
BsiHKCI CYCGRG 1 cut(s) 1676
BsiSI CCGG 5 cut(s) 475, 782, 1157, 1821, 1934
BslFI GGGAC 3 cut(s) 799, 1036, 2017
BslI CCNNNNNNNGG 7 cut(s) 508, 758, 788, 1482, 1577, 1607, 1990
BsmAI GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1468, 1508, 1827
BsmFI GGGAC 3 cut(s) 799, 1036, 2017
BsmI GAATGC 3 cut(s) 278, 934, 2188
BsoBI CYCGRG 1 cut(s) 1676
Bsp120I GGGCCC 1 cut(s) 1673
Bsp1286I GDGCHC 1 cut(s) 1677
Bsp143I GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
Bsp19I CCATGG 2 cut(s) 1252, 2199
BspACI CCGC 7 cut(s) 202, 204, 722, 1296, 1312, 1598, 2424
BspCNI CTCAG 9 cut(s) 49, 550, 577, 1104, 1351, 1807, 1956, 2098, 2332
BspDI ATCGAT 3 cut(s) 402, 737, 2258
BspFNI CGCG 6 cut(s) 204, 693, 1314, 1437, 1722, 1999
BspLI GGNNCC 5 cut(s) 6, 57, 1455, 1675, 2350
BspMI ACCTGC 1 cut(s) 869
BspPI GGATC 2 cut(s) 2343, 2356
BspQI GCTCTTC 2 cut(s) 56, 734
BspT107I GGYRCC 2 cut(s) 4, 1453
BspTI CTTAAG 1 cut(s) 1046
BsrDI GCAATG 1 cut(s) 1937
BsrFI RCCGGY 2 cut(s) 474, 1820
BsrI ACTGG 4 cut(s) 186, 793, 832, 1303
BssAI RCCGGY 2 cut(s) 474, 1820
BssECI CCNNGG 8 cut(s) 202, 331, 782, 991, 1252, 1530, 1626, 2199
BssMI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
BssNI GRCGYC 2 cut(s) 5, 1954
BssSI CACGAG 1 cut(s) 2337
BssT1I CCWWGG 4 cut(s) 331, 1252, 1626, 2199
Bst2BI CACGAG 1 cut(s) 2337
Bst2UI CCWGG 3 cut(s) 503, 1602, 1950
Bst4CI ACNGT 6 cut(s) 214, 995, 1444, 1519, 1891, 2084
Bst6I CTCTTC 5 cut(s) 49, 56, 734, 1623, 1769
BstACI GRCGYC 2 cut(s) 5, 1954
BstAFI CTTAAG 1 cut(s) 1046
BstAPI GCANNNNNTGC 2 cut(s) 1274, 2066
BstC8I GCNNGC 4 cut(s) 1639, 1741, 2125, 2241
BstDSI CCRYGG 5 cut(s) 202, 991, 1252, 1530, 2199
BstENI CCTNNNNNAGG 1 cut(s) 1575
BstFNI CGCG 6 cut(s) 204, 693, 1314, 1437, 1722, 1999
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 7 cut(s) 7, 577, 1060, 1437, 1722, 2001, 2028
BstKTI GATC 7 cut(s) 31, 496, 1228, 1273, 2235, 2351, 2449
BstMAI GTCTC 8 cut(s) 114, 201, 234, 539, 818, 1468, 1508, 1827
BstMBI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
BstMCI CGRYCG 1 cut(s) 2424
BstMWI GCNNNNNNNGC 7 cut(s) 1274, 1534, 1681, 1826, 2066, 2396, 2429
BstNI CCWGG 3 cut(s) 503, 1602, 1950
BstNSI RCATGY 3 cut(s) 1064, 1567, 2022
BstSCI CCNGG 5 cut(s) 501, 781, 1600, 1932, 1948
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 1677
BstUI CGCG 6 cut(s) 204, 693, 1314, 1437, 1722, 1999
BstV1I GCAGC 6 cut(s) 163, 736, 1264, 2072, 2341, 2386
BstV2I GAAGAC 4 cut(s) 42, 627, 1347, 2151
BstX2I RGATCY 2 cut(s) 1270, 2348
BstYI RGATCY 2 cut(s) 1270, 2348
BstZI CGGCCG 1 cut(s) 2421
Bsu15I ATCGAT 3 cut(s) 402, 737, 2258
BsuTUI ATCGAT 3 cut(s) 402, 737, 2258
BtgI CCRYGG 5 cut(s) 202, 991, 1252, 1530, 2199
BtsIMutI CAGTG 4 cut(s) 179, 210, 1310, 2103
BveI ACCTGC 1 cut(s) 869
Cac8I GCNNGC 4 cut(s) 1639, 1741, 2125, 2241
CaiI CAGNNNCTG 4 cut(s) 554, 584, 1027, 2432
CfoI GCGC 7 cut(s) 7, 577, 1060, 1437, 1722, 2001, 2028
Cfr10I RCCGGY 2 cut(s) 474, 1820
Cfr13I GGNCC 7 cut(s) 55, 682, 776, 854, 1595, 1673, 1674
Cfr42I CCGCGG 1 cut(s) 205
ClaI ATCGAT 3 cut(s) 402, 737, 2258
Csp6I GTAC 3 cut(s) 562, 1454, 2305
CspCI CAANNNNNGTGG 2 cut(s) 1512, 1547
CviAII CATG 9 cut(s) 514, 541, 1061, 1072, 1253, 1467, 1564, 2019, 2200
CviQI GTAC 3 cut(s) 562, 1454, 2305
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 30, 495, 1227, 1272, 2234, 2350, 2448
DpnII GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
DraI TTTAAA 1 cut(s) 2014
DrdI GACNNNNNNGTC 1 cut(s) 1408
DriI GACNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 1408
EaeI YGGCCR 1 cut(s) 2421
EagI CGGCCG 1 cut(s) 2421
Eam1104I CTCTTC 5 cut(s) 49, 56, 734, 1623, 1769
Eam1105I GACNNNNNGTC 1 cut(s) 125
EarI CTCTTC 5 cut(s) 49, 56, 734, 1623, 1769
EclXI CGGCCG 1 cut(s) 2421
Eco130I CCWWGG 4 cut(s) 331, 1252, 1626, 2199
Eco147I AGGCCT 1 cut(s) 1948
Eco24I GRGCYC 1 cut(s) 1677
Eco32I GATATC 1 cut(s) 2101
Eco47I GGWCC 3 cut(s) 55, 854, 1595
Eco52I CGGCCG 1 cut(s) 2421
Eco57I CTGAAG 2 cut(s) 176, 1988
Eco88I CYCGRG 1 cut(s) 1676
EcoNI CCTNNNNNAGG 1 cut(s) 1575
EcoO109I RGGNCCY 1 cut(s) 1673
EcoRI GAATTC 1 cut(s) 1369
EcoRII CCWGG 3 cut(s) 501, 1600, 1948
EcoRV GATATC 1 cut(s) 2101
EcoT14I CCWWGG 4 cut(s) 331, 1252, 1626, 2199
EcoT22I ATGCAT 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 1677
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 331, 1252, 1626, 2199
FaeI CATG 9 cut(s) 517, 544, 1064, 1075, 1256, 1470, 1567, 2022, 2203
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 3 cut(s) 799, 1036, 2017
FatI CATG 9 cut(s) 513, 540, 1060, 1071, 1252, 1466, 1563, 2018, 2199
Fnu4HI GCNGC 8 cut(s) 177, 725, 1278, 1296, 2061, 2330, 2400, 2424
FriOI GRGCYC 1 cut(s) 1677
Fsp4HI GCNGC 8 cut(s) 177, 725, 1278, 1296, 2061, 2330, 2400, 2424
FspAI RTGCGCAY 1 cut(s) 1059
FspBI CTAG 3 cut(s) 635, 1778, 2057
FspI TGCGCA 2 cut(s) 1059, 2027
GlaI GCGC 7 cut(s) 6, 576, 1059, 1436, 1721, 2000, 2027
GluI GCNGC 8 cut(s) 177, 725, 1278, 1296, 2061, 2330, 2400, 2424
GsaI CCCAGC 1 cut(s) 1024
GsuI CTGGAG 5 cut(s) 203, 849, 1286, 1851, 2454
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 5 cut(s) 475, 782, 1157, 1821, 1934
HhaI GCGC 7 cut(s) 7, 577, 1060, 1437, 1722, 2001, 2028
Hin1I GRCGYC 2 cut(s) 5, 1954
Hin1II CATG 9 cut(s) 517, 544, 1064, 1075, 1256, 1470, 1567, 2022, 2203
Hin6I GCGC 7 cut(s) 5, 575, 1058, 1435, 1720, 1999, 2026
HinP1I GCGC 7 cut(s) 5, 575, 1058, 1435, 1720, 1999, 2026
HincII GTYRAC 1 cut(s) 2117
HindII GTYRAC 1 cut(s) 2117
HindIII AAGCTT 2 cut(s) 188, 430
HinfI GANTC 9 cut(s) 13, 281, 467, 962, 1128, 1188, 1781, 1839, 2255
HpaII CCGG 5 cut(s) 475, 782, 1157, 1821, 1934
HphI GGTGA 3 cut(s) 503, 893, 1007
Hpy166II GTNNAC 2 cut(s) 388, 2117
Hpy188III TCNNGA 9 cut(s) 26, 1229, 1399, 1511, 1541, 1843, 1928, 1958, 2299
Hpy8I GTNNAC 2 cut(s) 388, 2117
HpyAV CCTTC 8 cut(s) 376, 404, 410, 481, 898, 1581, 2012, 2149
HpyCH4III ACNGT 6 cut(s) 214, 995, 1444, 1519, 1891, 2084
HpyCH4IV ACGT 4 cut(s) 616, 1494, 1954, 2119
HpyF10VI GCNNNNNNNGC 7 cut(s) 1274, 1534, 1681, 1826, 2066, 2396, 2429
HpySE526I ACGT 4 cut(s) 616, 1494, 1954, 2119
Hsp92I GRCGYC 2 cut(s) 5, 1954
Hsp92II CATG 9 cut(s) 517, 544, 1064, 1075, 1256, 1470, 1567, 2022, 2203
HspAI GCGC 7 cut(s) 5, 575, 1058, 1435, 1720, 1999, 2026
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 1457
KspI CCGCGG 1 cut(s) 205
Kzo9I GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
LguI GCTCTTC 2 cut(s) 56, 734
LmnI GCTCC 6 cut(s) 28, 830, 976, 1076, 1534, 1756
Lsp1109I GCAGC 6 cut(s) 163, 736, 1264, 2072, 2341, 2386
MaeI CTAG 3 cut(s) 635, 1778, 2057
MaeII ACGT 4 cut(s) 616, 1494, 1954, 2119
MaeIII GTNAC 3 cut(s) 421, 893, 1381
MalI GATC 7 cut(s) 30, 495, 1227, 1272, 2234, 2350, 2448
MboI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
MfeI CAATTG 3 cut(s) 411, 1632, 1785
MflI RGATCY 2 cut(s) 1270, 2348
MhlI GDGCHC 1 cut(s) 1677
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 3 cut(s) 22, 1775, 1848
MmeI TCCRAC 2 cut(s) 640, 1147
Mph1103I ATGCAT 1 cut(s) 445
MseI TTAA 6 cut(s) 218, 222, 849, 1034, 1047, 2013
MslI CAYNNNNRTG 3 cut(s) 1070, 1151, 1303
MspA1I CMGCKG 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 5 cut(s) 475, 782, 1157, 1821, 1934
MspR9I CCNGG 5 cut(s) 503, 783, 1602, 1934, 1950
MunI CAATTG 3 cut(s) 411, 1632, 1785
Mva1269I GAATGC 3 cut(s) 278, 934, 2188
MvaI CCWGG 3 cut(s) 503, 1602, 1950
MvnI CGCG 6 cut(s) 204, 693, 1314, 1437, 1722, 1999
MwoI GCNNNNNNNGC 7 cut(s) 1274, 1534, 1681, 1826, 2066, 2396, 2429
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 783, 1934
NcoI CCATGG 2 cut(s) 1252, 2199
NdeII GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
NlaIII CATG 9 cut(s) 517, 544, 1064, 1075, 1256, 1470, 1567, 2022, 2203
NlaIV GGNNCC 5 cut(s) 6, 57, 1455, 1675, 2350
NmeAIII GCCGAG 1 cut(s) 2254
NmuCI GTSAC 2 cut(s) 421, 1381
NsbI TGCGCA 2 cut(s) 1059, 2027
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 3 cut(s) 1064, 1567, 2022
OliI CACNNNNGTG 1 cut(s) 1303
PacI TTAATTAA 1 cut(s) 222
PaqCI CACCTGC 1 cut(s) 869
PceI AGGCCT 1 cut(s) 1948
PciSI GCTCTTC 2 cut(s) 56, 734
PctI GAATGC 3 cut(s) 278, 934, 2188
PfeI GAWTC 6 cut(s) 281, 467, 962, 1128, 1188, 2255
PflFI GACNNNGTC 1 cut(s) 1838
PkrI GCNGC 8 cut(s) 178, 726, 1279, 1297, 2062, 2331, 2401, 2425
PleI GAGTC 3 cut(s) 21, 1775, 1847
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 3 cut(s) 21, 1775, 1847
PshBI ATTAAT 1 cut(s) 849
Psp6I CCWGG 3 cut(s) 501, 1600, 1948
PspFI CCCAGC 1 cut(s) 1020
PspGI CCWGG 3 cut(s) 501, 1600, 1948
PspN4I GGNNCC 5 cut(s) 6, 57, 1455, 1675, 2350
PspOMI GGGCCC 1 cut(s) 1673
PspPI GGNCC 7 cut(s) 55, 682, 776, 854, 1595, 1673, 1674
PstNI CAGNNNCTG 4 cut(s) 554, 584, 1027, 2432
PsuI RGATCY 2 cut(s) 1270, 2348
PsyI GACNNNGTC 1 cut(s) 1838
RsaI GTAC 3 cut(s) 563, 1455, 2306
RsaNI GTAC 3 cut(s) 562, 1454, 2305
RseI CAYNNNNRTG 3 cut(s) 1070, 1151, 1303
SacII CCGCGG 1 cut(s) 205
SapI GCTCTTC 2 cut(s) 56, 734
SaqAI TTAA 6 cut(s) 218, 222, 849, 1034, 1047, 2013
SatI GCNGC 8 cut(s) 177, 725, 1278, 1296, 2061, 2330, 2400, 2424
Sau3AI GATC 7 cut(s) 28, 493, 1225, 1270, 2232, 2348, 2446
Sau96I GGNCC 7 cut(s) 55, 682, 776, 854, 1595, 1673, 1674
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 3 cut(s) 22, 1775, 1848
ScrFI CCNGG 5 cut(s) 503, 783, 1602, 1934, 1950
SduI GDGCHC 1 cut(s) 1677
SfcI CTRYAG 1 cut(s) 813
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 205
SinI GGWCC 3 cut(s) 55, 854, 1595
SmiMI CAYNNNNRTG 3 cut(s) 1070, 1151, 1303
SmlI CTYRAG 2 cut(s) 898, 1046
SmoI CTYRAG 2 cut(s) 898, 1046
SseBI AGGCCT 1 cut(s) 1948
SsiI CCGC 7 cut(s) 202, 204, 722, 1296, 1312, 1598, 2424
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 635, 1778, 2057
StuI AGGCCT 1 cut(s) 1948
StyD4I CCNGG 5 cut(s) 501, 781, 1600, 1932, 1948
StyI CCWWGG 4 cut(s) 331, 1252, 1626, 2199
TaaI ACNGT 6 cut(s) 214, 995, 1444, 1519, 1891, 2084
TaiI ACGT 4 cut(s) 619, 1497, 1957, 2122
TatI WGTACW 1 cut(s) 561
TauI GCSGC 2 cut(s) 1298, 2426
TfiI GAWTC 6 cut(s) 281, 467, 962, 1128, 1188, 2255
Tru1I TTAA 6 cut(s) 218, 222, 849, 1034, 1047, 2013
Tru9I TTAA 6 cut(s) 218, 222, 849, 1034, 1047, 2013
TscAI CASTG 4 cut(s) 186, 217, 1310, 2110
TseFI GTSAC 2 cut(s) 421, 1381
TseI GCWGC 6 cut(s) 176, 724, 1277, 2060, 2329, 2399
Tsp45I GTSAC 2 cut(s) 421, 1381
TspRI CASTG 4 cut(s) 186, 217, 1310, 2110
Tth111I GACNNNGTC 1 cut(s) 1838
Vha464I CTTAAG 1 cut(s) 1046
VpaK11BI GGWCC 3 cut(s) 55, 854, 1595
VspI ATTAAT 1 cut(s) 849
XagI CCTNNNNNAGG 1 cut(s) 1575
XapI RAATTY 4 cut(s) 1369, 1556, 1804, 2354
XceI RCATGY 3 cut(s) 1064, 1567, 2022
XcmI CCANNNNNNNNNTGG 2 cut(s) 1259, 1464
XspI CTAG 3 cut(s) 635, 1778, 2057
ZraI GACGTC 1 cut(s) 1955
ZrmI AGTACT 1 cut(s) 563
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.