pycom14g15610

two-component response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012837
Physical Location & Seq
Forward (+)
18191379 .. 18193309
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGGCGACGGCAGGCGAGATTCTCAGGCGGAATCTGACCGAAAGTTTTGATCTTTCCGGTGATTCGCAGATGGGTTCCGGCGAGCTGCATGTTCTTGCTGTGGATGACAGCCACGTGGACCGGAAGGTCATTGAACGCCTGCTAAAGATATCATCCTGCAAAGTGACGGCCGTTGACAGTGGGACGAGAGCTCTGGAGTATCTGGGCTTGGATGGAGAGAAGAGCTCGGTTGGATTTGATAACATGAAGGTGAATTTGATCATAACGGACTACTCAATGCCAGGGATGACAGGATATGAACTGCTAAAGAAAATCAAGGAATCATCGGCTTTTCGAGAAGTTCCGGTGGTGATCATGTCATCGGAAAACATCCTTACTCGTATTGATAGATGTTTAGAGGAAGGAGCTGAGGAATATATAGTGAAGCCGGTGAAGCTTTCCGACGTGAACCGTTTAAAAAACTTCATGCTGGGAGGAGAAAGAAAAGAAACCGAAGAGAAAATGATTCACAAAAGAACATTTTCAGTTGACTACAATGCATCATCATCTCCCTCCTCTTTACCACAACTTTCGCAACCGTTCGCTTGCAATCTAGCATCGTCTCAGCTACCACCTTTGTCACCGTCATCGTCATTGCCACGATGTTCGTCAAAGAGACCGAGATTGCACACGATGGATTGA

Protein Analysis

227

Amino Acids

25.01

Weight (kDa)

6.14

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 31 - 148 3e-21 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48100 AT5G62920
fragaria_vesca FvH4_5g16240
malus_domestica MD06G1182400.v1.1 MD14G1188400.v1.1
prunus_persica Prupe.5G180500_v2.0.a1 Prupe.5G180500_v2.0.a1
pyrus_communis pycom06g16130 pycom14g15610
rosa_chinensis RchiOBHm_Chr7g0179851
rosa_laevigata RLG00000005318
rosa_roxburghii Rroxscaffold_3G00273400
rosa_rugosa Rorug06G0428800
rosa_samantha Rh7AG028600 Rh7BG027800 Rh7CG029500 Rh7DG028900
rosa_wichuraiana Rw7G002290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AciI CCGC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 253
AcvI CACGTG 1 cut(s) 115
AgsI TTSAA 1 cut(s) 134
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 6 cut(s) 85, 191, 225, 407, 436, 607
AluI AGCT 6 cut(s) 85, 191, 225, 407, 436, 607
Alw21I GWGCWC 2 cut(s) 193, 227
Alw26I GTCTC 2 cut(s) 606, 649
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 1 cut(s) 85
ApoI RAATTY 1 cut(s) 253
Asp700I GAANNNNTTC 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 5 cut(s) 71, 262, 361, 442, 612
AvaII GGWCC 1 cut(s) 118
BanII GRGCYC 2 cut(s) 193, 227
BbrPI CACGTG 1 cut(s) 115
Bbv12I GWGCWC 2 cut(s) 193, 227
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 72
BccI CCATC 3 cut(s) 64, 206, 667
BceAI ACGGC 3 cut(s) 24, 155, 183
BciT130I CCWGG 1 cut(s) 282
BclI TGATCA 2 cut(s) 258, 351
BcoDI GTCTC 2 cut(s) 606, 649
BfaI CTAG 1 cut(s) 593
BisI GCNGC 1 cut(s) 86
BlsI GCNGC 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 1 cut(s) 118
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 2 cut(s) 548, 605
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 115
BsaI GGTCTC 1 cut(s) 649
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 3 cut(s) 56, 120, 343
Bse118I RCCGGY 1 cut(s) 427
Bse3DI GCAATG 1 cut(s) 632
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 5 cut(s) 109, 152, 217, 291, 369
BseMI GCAATG 1 cut(s) 632
BseMII CTCAG 3 cut(s) 37, 399, 617
BseRI GAGGAG 2 cut(s) 489, 544
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 1 cut(s) 72
BseYI CCCAGC 1 cut(s) 469
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 1 cut(s) 170
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 193, 227
BsiSI CCGG 5 cut(s) 57, 78, 121, 344, 428
BslFI GGGAC 1 cut(s) 196
BsmAI GTCTC 2 cut(s) 606, 649
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 196
BsnI GGCC 1 cut(s) 170
Bso31I GGTCTC 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 193, 227
Bsp143I GATC 3 cut(s) 49, 258, 351
BspACI CCGC 1 cut(s) 28
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 3 cut(s) 36, 400, 616
BspLI GGNNCC 1 cut(s) 76
BspQI GCTCTTC 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 649
BsrDI GCAATG 1 cut(s) 632
BsrFI RCCGGY 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 3 cut(s) 49, 258, 351
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 4 cut(s) 179, 452, 579, 624
Bst6I CTCTTC 2 cut(s) 215, 489
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 4 cut(s) 13, 83, 140, 586
BstDEI CTNAG 3 cut(s) 23, 408, 603
BstF5I GGATG 5 cut(s) 109, 152, 217, 291, 369
BstKTI GATC 3 cut(s) 52, 261, 354
BstMAI GTCTC 2 cut(s) 606, 649
BstMBI GATC 3 cut(s) 49, 258, 351
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 72
BstZI CGGCCG 1 cut(s) 168
BsuRI GGCC 1 cut(s) 170
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 5 cut(s) 109, 152, 217, 291, 369
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 4 cut(s) 13, 83, 140, 586
Cfr10I RCCGGY 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 118
CviAII CATG 4 cut(s) 89, 244, 355, 466
DdeI CTNAG 3 cut(s) 23, 408, 603
DpnI GATC 3 cut(s) 51, 260, 353
DpnII GATC 3 cut(s) 49, 258, 351
DraI TTTAAA 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 168
EagI CGGCCG 1 cut(s) 168
Eam1104I CTCTTC 2 cut(s) 215, 489
EarI CTCTTC 2 cut(s) 215, 489
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 2 cut(s) 191, 225
EclXI CGGCCG 1 cut(s) 168
Eco24I GRGCYC 2 cut(s) 193, 227
Eco31I GGTCTC 1 cut(s) 649
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 118
Eco52I CGGCCG 1 cut(s) 168
Eco53kI GAGCTC 2 cut(s) 191, 225
Eco72I CACGTG 1 cut(s) 115
EcoICRI GAGCTC 2 cut(s) 191, 225
EcoRII CCWGG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 541
EcoT38I GRGCYC 2 cut(s) 193, 227
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 4 cut(s) 92, 247, 358, 469
FaiI YATR 8 cut(s) 90, 245, 263, 297, 356, 417, 419, 467
FaqI GGGAC 1 cut(s) 196
FatI CATG 4 cut(s) 88, 243, 354, 465
FbaI TGATCA 2 cut(s) 258, 351
Fnu4HI GCNGC 1 cut(s) 86
FokI GGATG 5 cut(s) 116, 139, 224, 298, 356
FriOI GRGCYC 2 cut(s) 193, 227
Fsp4HI GCNGC 1 cut(s) 86
FspBI CTAG 1 cut(s) 593
GluI GCNGC 1 cut(s) 86
GsaI CCCAGC 1 cut(s) 473
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 170
HapII CCGG 5 cut(s) 57, 78, 121, 344, 428
Hin1II CATG 4 cut(s) 92, 247, 358, 469
HincII GTYRAC 2 cut(s) 175, 529
HindII GTYRAC 2 cut(s) 175, 529
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 5 cut(s) 19, 31, 62, 320, 505
HpaII CCGG 5 cut(s) 57, 78, 121, 344, 428
HphI GGTGA 5 cut(s) 71, 262, 361, 442, 612
Hpy166II GTNNAC 4 cut(s) 118, 175, 448, 529
Hpy188I TCNGA 3 cut(s) 36, 364, 442
Hpy188III TCNNGA 2 cut(s) 194, 335
Hpy8I GTNNAC 4 cut(s) 118, 175, 448, 529
Hpy99I CGWCG 2 cut(s) 10, 446
HpyAV CCTTC 3 cut(s) 118, 241, 395
HpyCH4III ACNGT 4 cut(s) 179, 452, 579, 624
HpyCH4IV ACGT 2 cut(s) 114, 444
HpyCH4V TGCA 5 cut(s) 88, 159, 539, 588, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpyF3I CTNAG 3 cut(s) 23, 408, 603
HpySE526I ACGT 2 cut(s) 114, 444
Hsp92II CATG 4 cut(s) 92, 247, 358, 469
Ksp22I TGATCA 2 cut(s) 258, 351
Kzo9I GATC 3 cut(s) 49, 258, 351
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 2 cut(s) 548, 605
MaeI CTAG 1 cut(s) 593
MaeII ACGT 2 cut(s) 114, 444
MaeIII GTNAC 2 cut(s) 163, 618
MalI GATC 3 cut(s) 51, 260, 353
MboI GATC 3 cut(s) 49, 258, 351
MboII GAAGA 2 cut(s) 232, 506
MhlI GDGCHC 2 cut(s) 193, 227
MluCI AATT 1 cut(s) 253
MmeI TCCRAC 2 cut(s) 211, 465
MnlI CCTC 5 cut(s) 391, 403, 467, 562, 565
Mph1103I ATGCAT 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 520
MseI TTAA 1 cut(s) 455
MslI CAYNNNNRTG 1 cut(s) 248
MspI CCGG 5 cut(s) 57, 78, 121, 344, 428
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 433
NdeII GATC 3 cut(s) 49, 258, 351
NlaIII CATG 4 cut(s) 92, 247, 358, 469
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 163, 618
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 1 cut(s) 92
PciSI GCTCTTC 1 cut(s) 215
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 5 cut(s) 19, 31, 62, 320, 505
PkrI GCNGC 1 cut(s) 87
PmaCI CACGTG 1 cut(s) 115
PmlI CACGTG 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 115
Psp124BI GAGCTC 2 cut(s) 193, 227
Psp6I CCWGG 1 cut(s) 280
PspCI CACGTG 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 248
SacI GAGCTC 2 cut(s) 193, 227
SapI GCTCTTC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 455
SatI GCNGC 1 cut(s) 86
Sau3AI GATC 3 cut(s) 49, 258, 351
Sau96I GGNCC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 2 cut(s) 193, 227
SfaNI GCATC 2 cut(s) 548, 605
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 1 cut(s) 253
SsiI CCGC 1 cut(s) 28
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 2 cut(s) 193, 227
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 4 cut(s) 179, 452, 579, 624
TaiI ACGT 2 cut(s) 117, 447
TaqI TCGA 1 cut(s) 334
TaqII GACCGA 2 cut(s) 53, 673
TasI AATT 1 cut(s) 253
TfiI GAWTC 5 cut(s) 19, 31, 62, 320, 505
Tru1I TTAA 1 cut(s) 455
Tru9I TTAA 1 cut(s) 455
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 2 cut(s) 163, 618
TseI GCWGC 1 cut(s) 85
Tsp45I GTSAC 2 cut(s) 163, 618
TspDTI ATGAA 3 cut(s) 260, 312, 454
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 253
XceI RCATGY 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 1 cut(s) 593
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.