Rh7DG028900

two-component response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012837
Physical Location & Seq
Forward (+)
2175728 .. 2178157
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG028900.1

Sequence Viewer

Length: 495 bp
ATGGCCACCGCCGGTGAGGTTTTAAGGCGAAATGTGCCGGAAATGACTGCCGTTGAGAGTGGAACAAGGGCTCTGCAGTATCTGGGCTTGGATGGAGAGAAGAAGAGCTCAGTTGGATTTGATGGTATGAAGGTGAATCTAATAATAACAGACTACTCAATGCCTGGGATGACAGGATATGAGCTGCTAAAAAAGATCAAGGAATCATCAGCTTTTAGAGAAGTTCCAGTGGTGATCATGTCCTCAGAGAACATCCTCACTCGAATCGATAGATGTTTGGAGGAAGGAGCTGAAGAGTTTATAGTAAAGCCGGTAAAACTTTCAGATGTGAACCGCTTGAAAAATTTCATGATGGGAGGAGAAAGAAAAGAGAAAGGAGTGAAGAAGGTTCAGAAAAGACCGCTTCAAGTTGACTACAACAATTCTTCATCGGCACCCTCGCCTTTACCACAACTGTCACCATCATTTGCTTATGTAGACCCTCTACATTATTAG

Protein Analysis

164

Amino Acids

18.26

Weight (kDa)

8.59

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 41 - 109 3.3e-15 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012837)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48100 AT5G62920
fragaria_vesca FvH4_5g16240
malus_domestica MD06G1182400.v1.1 MD14G1188400.v1.1
prunus_persica Prupe.5G180500_v2.0.a1 Prupe.5G180500_v2.0.a1
pyrus_communis pycom06g16130 pycom14g15610
rosa_chinensis RchiOBHm_Chr7g0179851
rosa_laevigata RLG00000005318
rosa_roxburghii Rroxscaffold_3G00273400
rosa_rugosa Rorug06G0428800
rosa_samantha Rh7AG028600 Rh7BG027800 Rh7CG029500 Rh7DG028900
rosa_wichuraiana Rw7G002290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 477
AciI CCGC 3 cut(s) 9, 334, 401
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 312
AgsI TTSAA 2 cut(s) 340, 407
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 4 cut(s) 108, 184, 212, 290
AluI AGCT 4 cut(s) 108, 184, 212, 290
Alw21I GWGCWC 1 cut(s) 110
AlwNI CAGNNNCTG 1 cut(s) 82
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 1 cut(s) 343
Asp700I GAANNNNTTC 1 cut(s) 344
AsuHPI GGTGA 4 cut(s) 26, 145, 244, 450
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 433
BanII GRGCYC 2 cut(s) 73, 110
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 171
BccI CCATC 4 cut(s) 86, 116, 346, 469
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 164
Bse118I RCCGGY 2 cut(s) 11, 310
Bse1I ACTGG 1 cut(s) 227
BseBI CCWGG 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 164
BseGI GGATG 3 cut(s) 97, 174, 252
BseMII CTCAG 2 cut(s) 123, 258
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 171
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 433
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 110
BsiSI CCGG 3 cut(s) 12, 38, 311
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 73, 110
Bsp143I GATC 2 cut(s) 195, 234
BspACI CCGC 3 cut(s) 9, 334, 401
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 122, 257
BspDI ATCGAT 1 cut(s) 267
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 433
BsrFI RCCGGY 2 cut(s) 11, 310
BsrI ACTGG 1 cut(s) 227
BssAI RCCGGY 2 cut(s) 11, 310
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 2 cut(s) 195, 234
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 456
Bst6I CTCTTC 2 cut(s) 98, 288
BstDEI CTNAG 2 cut(s) 109, 244
BstF5I GGATG 3 cut(s) 97, 174, 252
BstKTI GATC 2 cut(s) 198, 237
BstMBI GATC 2 cut(s) 195, 234
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 3 cut(s) 97, 174, 252
BtsIMutI CAGTG 1 cut(s) 234
CaiI CAGNNNCTG 1 cut(s) 82
CciI TCATGA 1 cut(s) 348
Cfr10I RCCGGY 2 cut(s) 11, 310
ClaI ATCGAT 1 cut(s) 267
CviAII CATG 2 cut(s) 238, 349
CviJI RGCY 8 cut(s) 5, 71, 87, 108, 184, 212, 290, 310
CviKI_1 RGCY 8 cut(s) 5, 71, 87, 108, 184, 212, 290, 310
DdeI CTNAG 2 cut(s) 109, 244
DpnI GATC 2 cut(s) 197, 236
DpnII GATC 2 cut(s) 195, 234
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 98, 288
EarI CTCTTC 2 cut(s) 98, 288
Ecl136II GAGCTC 1 cut(s) 108
Eco24I GRGCYC 2 cut(s) 73, 110
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 163
EcoT38I GRGCYC 2 cut(s) 73, 110
FaeI CATG 2 cut(s) 241, 352
FaiI YATR 6 cut(s) 128, 180, 239, 302, 350, 474
FatI CATG 2 cut(s) 237, 348
FbaI TGATCA 1 cut(s) 234
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 185
FokI GGATG 3 cut(s) 104, 181, 239
FriOI GRGCYC 2 cut(s) 73, 110
Fsp4HI GCNGC 1 cut(s) 185
GluI GCNGC 1 cut(s) 185
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 12, 38, 311
Hin1II CATG 2 cut(s) 241, 352
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 3 cut(s) 136, 203, 264
HpaII CCGG 3 cut(s) 12, 38, 311
HphI GGTGA 4 cut(s) 26, 145, 244, 450
Hpy166II GTNNAC 3 cut(s) 331, 412, 478
Hpy188I TCNGA 3 cut(s) 247, 325, 393
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 3 cut(s) 331, 412, 478
HpyAV CCTTC 3 cut(s) 124, 278, 379
HpyCH4III ACNGT 1 cut(s) 456
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 2 cut(s) 109, 244
Hsp92II CATG 2 cut(s) 241, 352
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 2 cut(s) 195, 234
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 8 cut(s) 25, 51, 68, 150, 159, 177, 240, 324
Lsp1109I GCAGC 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 456
MalI GATC 2 cut(s) 197, 236
MboI GATC 2 cut(s) 195, 234
MboII GAAGA 5 cut(s) 112, 115, 305, 394, 417
MhlI GDGCHC 2 cut(s) 73, 110
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 343, 421
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 7 cut(s) 10, 253, 266, 274, 350, 448, 492
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 344
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 12, 38, 311
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 1 cut(s) 34
NdeII GATC 2 cut(s) 195, 234
NlaIII CATG 2 cut(s) 241, 352
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 456
PagI TCATGA 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 3 cut(s) 136, 203, 264
PkrI GCNGC 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 435
PstI CTGCAG 1 cut(s) 78
PstNI CAGNNNCTG 1 cut(s) 82
SacI GAGCTC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 2 cut(s) 195, 234
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 2 cut(s) 73, 110
SetI ASST 7 cut(s) 21, 110, 135, 186, 214, 292, 390
SfcI CTRYAG 1 cut(s) 74
SgrAI CRCCGGYG 1 cut(s) 11
Sse9I AATT 2 cut(s) 343, 421
SsiI CCGC 3 cut(s) 9, 334, 401
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 456
TaqI TCGA 2 cut(s) 262, 267
TasI AATT 2 cut(s) 343, 421
TfiI GAWTC 3 cut(s) 136, 203, 264
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 1 cut(s) 456
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 456
TspDTI ATGAA 3 cut(s) 143, 337, 417
TspRI CASTG 1 cut(s) 234
XapI RAATTY 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 477
XmnI GAANNNNTTC 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.