pycom14g16750

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010978
Physical Location & Seq
Reverse (-)
18936918 .. 18938161
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 855 bp
ATGGCCAAGGCATTTGCATCCCAGAGTGGTTTCTGCATTTCTTCTGGGTTGGATGAAATTGCAGTTGGCTCCAAATCCCCCAAAGGGGAATTCAGGGTTTCTCCCTCCTTCAGTACCTCACAATTTAAGGATGCAAATTCAAGACCTTTTCCTTCATTGAGTGTTGATTTCTTGGGAAAACCCATGACTCTCTCCGATCACAAGGGTTTTAAGAATTGGAGGGTCAGAAAACCTAGCAATTTCTCTGTGCATGCTCAAGCATCTGTCTGTGTAAGCAGAGCTATGAGGTGGTGGGAAAAGACCCTTCACCCCAACATGGTGGAGATCCATTCCGCTCAAGAACTTGTTGATTCCCTGAAAAATGCTGGGGACAGATTGGTTGTAGTTGACTTTTACTCACCTGGCTGTGGAGGCTGCAGAGCTCTCCATCCCAAGATCTGTCAGCTGGCTGAATCGAACCCGAATGCGATTTTCCTCAAAGTTAACTATGAACAGCACAAGACTATGTGTCATGCTCTCAACATTCATGTGCTGCCGTTCTTTAGATTCTTTAGAGGCGCAGATGGTCGCGTGTGCAGCTTTAGCTGCACCAATGCAACTATTAAGAAATTCAAAGATGCATTGGCGAAACACGGGTCCGATCGTTGTAATCTTGGGCCGGCGAAAGGGTTGGATGAATCTGAGATACTAAAGTTAGCTTCAATAGGTGAAATATCAACAACAACATCATCACCTTCCACGAAGGAAGAAAGATTGGAGGATCTGCTCACGAAAAGCGTAGATTTTTCGGGTGCTTGGAGCAATGCAGGCCATAGCATAGAACTCGAGGAAGAAAATTCCATGGTGAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

285

Amino Acids

31.24

Weight (kDa)

8.68

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 113 - 190 1.5e-13 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 335
AccII CGCG 1 cut(s) 570
AciI CCGC 1 cut(s) 333
AclWI GGATC 2 cut(s) 319, 768
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 89, 136, 608, 835
AcuI CTGAAG 1 cut(s) 94
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 5 cut(s) 84, 85, 316, 407, 665
AgsI TTSAA 3 cut(s) 141, 613, 702
AhdI GACNNNNNGTC 1 cut(s) 507
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 4 cut(s) 48, 74, 80, 106
AluBI AGCT 6 cut(s) 281, 422, 445, 579, 585, 698
AluI AGCT 6 cut(s) 281, 422, 445, 579, 585, 698
Alw21I GWGCWC 1 cut(s) 424
AlwI GGATC 2 cut(s) 319, 768
Ama87I CYCGRG 1 cut(s) 824
AoxI GGCC 3 cut(s) 3, 656, 808
ApeKI GCWGC 4 cut(s) 414, 532, 576, 585
ApoI RAATTY 4 cut(s) 89, 136, 608, 835
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AspLEI GCGC 1 cut(s) 560
AspS9I GGNCC 2 cut(s) 636, 656
AsuHPI GGTGA 4 cut(s) 299, 390, 719, 723
AvaI CYCGRG 1 cut(s) 824
AvaII GGWCC 1 cut(s) 636
BaeI ACNNNNGTAYC 2 cut(s) 677, 710
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 4 cut(s) 401, 519, 572, 588
BccI CCATC 2 cut(s) 435, 557
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 2 cut(s) 805, 839
BciT130I CCWGG 1 cut(s) 402
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 415
BglII AGATCT 1 cut(s) 435
BisI GCNGC 4 cut(s) 415, 533, 577, 586
BlsI GCNGC 4 cut(s) 416, 534, 578, 587
Bme1390I CCNGG 1 cut(s) 402
Bme18I GGWCC 1 cut(s) 636
BmeRI GACNNNNNGTC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 824
BmgT120I GGNCC 2 cut(s) 636, 656
BmiI GGNNCC 2 cut(s) 70, 637
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 4 cut(s) 26, 121, 269, 607
BpuEI CTTGAG 2 cut(s) 240, 321
BsaJI CCNNGG 2 cut(s) 6, 840
Bsc4I CCNNNNNNNGG 5 cut(s) 84, 85, 316, 407, 665
Bse118I RCCGGY 1 cut(s) 658
Bse3DI GCAATG 1 cut(s) 808
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 6, 840
BseGI GGATG 5 cut(s) 17, 58, 136, 427, 679
BseLI CCNNNNNNNGG 5 cut(s) 84, 85, 316, 407, 665
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 1 cut(s) 672
BseXI GCAGC 4 cut(s) 401, 519, 572, 588
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 2 cut(s) 571, 595
Bsh1236I CGCG 1 cut(s) 570
Bsh1285I CGRYCG 1 cut(s) 643
BshFI GGCC 3 cut(s) 5, 658, 810
BsiEI CGRYCG 1 cut(s) 643
BsiHKAI GWGCWC 1 cut(s) 424
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 5 cut(s) 84, 85, 316, 407, 665
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 3 cut(s) 5, 658, 810
BsoBI CYCGRG 1 cut(s) 824
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 5 cut(s) 196, 324, 435, 640, 760
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 1 cut(s) 333
BspANI GGCC 3 cut(s) 5, 658, 810
BspCNI CTCAG 1 cut(s) 673
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 70, 637
BspMAI CTGCAG 1 cut(s) 419
BspPI GGATC 2 cut(s) 319, 768
BsrBI CCGCTC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 808
BsrFI RCCGGY 1 cut(s) 658
BssAI RCCGGY 1 cut(s) 658
BssECI CCNNGG 2 cut(s) 6, 840
BssMI GATC 5 cut(s) 196, 324, 435, 640, 760
BssT1I CCWWGG 2 cut(s) 6, 840
Bst2UI CCWGG 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 252, 447, 660, 808
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 5 cut(s) 17, 58, 136, 427, 679
BstFNI CGCG 1 cut(s) 570
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 5 cut(s) 199, 327, 438, 643, 763
BstMBI GATC 5 cut(s) 196, 324, 435, 640, 760
BstMCI CGRYCG 1 cut(s) 643
BstMWI GCNNNNNNNGC 5 cut(s) 411, 576, 582, 585, 807
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 415
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 4 cut(s) 401, 519, 572, 588
BstX2I RGATCY 3 cut(s) 324, 435, 760
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 3 cut(s) 324, 435, 760
BsuRI GGCC 3 cut(s) 5, 658, 810
BtgI CCRYGG 1 cut(s) 840
BtsCI GGATG 5 cut(s) 17, 58, 136, 427, 679
Cac8I GCNNGC 4 cut(s) 252, 447, 660, 808
CfoI GCGC 1 cut(s) 560
Cfr10I RCCGGY 1 cut(s) 658
Cfr13I GGNCC 2 cut(s) 636, 656
Csp6I GTAC 1 cut(s) 114
CviAII CATG 6 cut(s) 184, 251, 316, 512, 527, 841
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 681
DpnI GATC 5 cut(s) 198, 326, 437, 642, 762
DpnII GATC 5 cut(s) 196, 324, 435, 640, 760
DriI GACNNNNNGTC 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 507
Ecl136II GAGCTC 1 cut(s) 422
Eco130I CCWWGG 2 cut(s) 6, 840
Eco24I GRGCYC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 636
Eco53kI GAGCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 824
EcoICRI GAGCTC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 6, 840
EcoT22I ATGCAT 1 cut(s) 622
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 2 cut(s) 6, 840
FaeI CATG 6 cut(s) 187, 254, 319, 515, 530, 844
FaqI GGGAC 1 cut(s) 383
FatI CATG 6 cut(s) 183, 250, 315, 511, 526, 840
Fnu4HI GCNGC 4 cut(s) 415, 533, 577, 586
FokI GGATG 5 cut(s) 4, 65, 143, 414, 686
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 4 cut(s) 415, 533, 577, 586
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 559
GluI GCNGC 4 cut(s) 415, 533, 577, 586
GsaI CCCAGC 1 cut(s) 369
HaeIII GGCC 3 cut(s) 5, 658, 810
HapII CCGG 1 cut(s) 659
HhaI GCGC 1 cut(s) 560
Hin1II CATG 6 cut(s) 187, 254, 319, 515, 530, 844
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HincII GTYRAC 2 cut(s) 388, 484
HindII GTYRAC 2 cut(s) 388, 484
HinfI GANTC 5 cut(s) 187, 350, 452, 546, 677
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 1 cut(s) 659
HphI GGTGA 4 cut(s) 299, 390, 719, 723
Hpy166II GTNNAC 2 cut(s) 388, 484
Hpy188I TCNGA 4 cut(s) 196, 227, 640, 682
Hpy188III TCNNGA 3 cut(s) 141, 338, 769
Hpy8I GTNNAC 2 cut(s) 388, 484
HpyAV CCTTC 5 cut(s) 118, 162, 314, 736, 744
HpyF10VI GCNNNNNNNGC 5 cut(s) 411, 576, 582, 585, 807
HpyF3I CTNAG 1 cut(s) 681
Hsp92II CATG 6 cut(s) 187, 254, 319, 515, 530, 844
HspAI GCGC 1 cut(s) 558
KroI GCCGGC 1 cut(s) 658
KroNI GCCGGC 1 cut(s) 660
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 5 cut(s) 196, 324, 435, 640, 760
LmnI GCTCC 2 cut(s) 74, 798
Lsp1109I GCAGC 4 cut(s) 401, 519, 572, 588
LweI GCATC 4 cut(s) 26, 121, 269, 607
MaeI CTAG 1 cut(s) 234
MalI GATC 5 cut(s) 198, 326, 437, 642, 762
MbiI CCGCTC 1 cut(s) 335
MboI GATC 5 cut(s) 196, 324, 435, 640, 760
MboII GAAGA 3 cut(s) 33, 758, 842
MflI RGATCY 3 cut(s) 324, 435, 760
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 8 cut(s) 57, 89, 122, 136, 214, 238, 608, 835
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 2 cut(s) 30, 651
MnlI CCTC 9 cut(s) 115, 127, 213, 279, 404, 485, 548, 751, 820
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 622
MroNI GCCGGC 1 cut(s) 658
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 126, 210, 483, 603, 853
MslI CAYNNNNRTG 1 cut(s) 527
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 1 cut(s) 659
MspR9I CCNGG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 469
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 5 cut(s) 411, 576, 582, 585, 807
NaeI GCCGGC 1 cut(s) 660
NcoI CCATGG 1 cut(s) 840
NdeII GATC 5 cut(s) 196, 324, 435, 640, 760
NgoMIV GCCGGC 1 cut(s) 658
NlaIII CATG 6 cut(s) 187, 254, 319, 515, 530, 844
NlaIV GGNNCC 2 cut(s) 70, 637
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 1 cut(s) 254
PaeI GCATGC 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 824
PctI GAATGC 1 cut(s) 469
PdiI GCCGGC 1 cut(s) 660
PfeI GAWTC 4 cut(s) 350, 452, 546, 677
PkrI GCNGC 4 cut(s) 416, 534, 578, 587
Ple19I CGATCG 1 cut(s) 643
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 70, 637
PspPI GGNCC 2 cut(s) 636, 656
PspXI VCTCGAGB 1 cut(s) 824
PstI CTGCAG 1 cut(s) 419
PsuI RGATCY 3 cut(s) 324, 435, 760
PvuI CGATCG 1 cut(s) 643
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 527
SacI GAGCTC 1 cut(s) 424
SaqAI TTAA 5 cut(s) 126, 210, 483, 603, 853
SatI GCNGC 4 cut(s) 415, 533, 577, 586
Sau3AI GATC 5 cut(s) 196, 324, 435, 640, 760
Sau96I GGNCC 2 cut(s) 636, 656
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 4 cut(s) 26, 121, 269, 607
SfcI CTRYAG 1 cut(s) 415
Sfr274I CTCGAG 1 cut(s) 824
SinI GGWCC 1 cut(s) 636
SlaI CTCGAG 1 cut(s) 824
SmiMI CAYNNNNRTG 1 cut(s) 527
SmlI CTYRAG 3 cut(s) 255, 336, 824
SmoI CTYRAG 3 cut(s) 255, 336, 824
SphI GCATGC 1 cut(s) 254
Sse9I AATT 8 cut(s) 57, 89, 122, 136, 214, 238, 608, 835
SsiI CCGC 1 cut(s) 333
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 2 cut(s) 6, 840
TaqI TCGA 2 cut(s) 455, 825
TasI AATT 8 cut(s) 57, 89, 122, 136, 214, 238, 608, 835
TfiI GAWTC 4 cut(s) 350, 452, 546, 677
Tru1I TTAA 5 cut(s) 126, 210, 483, 603, 853
Tru9I TTAA 5 cut(s) 126, 210, 483, 603, 853
TseI GCWGC 4 cut(s) 414, 532, 576, 585
TspDTI ATGAA 5 cut(s) 69, 144, 504, 515, 690
VpaK11BI GGWCC 1 cut(s) 636
XapI RAATTY 4 cut(s) 89, 136, 608, 835
XceI RCATGY 1 cut(s) 254
XhoI CTCGAG 1 cut(s) 824
XspI CTAG 1 cut(s) 234
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.