pycom14g17210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006932
Physical Location & Seq
Reverse (-)
19247183 .. 19253863
6681 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4935 bp
ATGGAGAACAGTGGAGAAGCAGCAGATGATGAGGGCTCTGGATGGTTCGAAGTGAAAAAGAAAAATAGAAGTAGCTCAAAGTTCTCTCTACAGAGTTGGGTTGGGGGGTTTTCTGGAAAAAATGCTTCTAACCATTCGAGTAGTCGGACCTCTACAAGTGAAAGCAGTGGATATTCTCGGGACAAGCGCAGATCCCAGCCTCCAAAGATACCGGGAAACTATGGTGTATATAGCCGGGGAAGTGATGCAAATTCTATTGCTGTCCCAAATGAAAAGAAACCGGGTGCTCCCTATCATGATATCTGTGTTATTAAGCAAGATACTGGATGTCCTAAGCCACCTCCTTCCTTCGTTACAAATTCTGATGATGGAACTATAGATGCTGAGAAAATCCCTGCAAACGATAACTCCGAAGTAGTTGCTAAAATCAGGTGGGGGGATCTCGAGGGTGATGGTTTGGCACTCCCTTGTGCTAACCTTGTCGGTGCCAAAATCAAGTTTGGTGCTATCGGAGATGATAATCTGGTGGTCTCGAGTGAGCATGGGAATGGTCATAAGTTTGTTTCCTCTACAAATTTACAAAAAAACACATTGGTTGCAGAATCTGTGGATGCGAAGGATCAATTATGTGAAGATAAATGTAAGGAGGTGAATATAATCTCTTCTGAAAAGGCTGAAGAGCCAATTTTGAACAAAAAAAAGCTTGATTTTGATAACAGTACTTCGAATTGCAAGGATATAGATACTGAACACGTTGAAGTAGTAGCTGATGATAGTTTAAGTGCCAGTCCTTTAGCTGGTGAAGAGGCTGCGACAGTGGAGAAATGTCAGACACCTGTTGTTCTACCTGAGGTAGGTGACCATGAAATTCCTGAAGTATCAGTGATAACTGGGGATTCAATTGAAGTTCATGTTTCCGAAGATAAAGGGTTAATTCCACCAAAGAGGGATCATGGAATTTCTGAGGTGTCTACTTACACAGCCTCCGATGAGGACCATGAGGGTACACAATGTGGTATGATACATGATATGTCAAACTCACAAAATATGAGTGCCCTTGGAGAATGTGACACAGATGAAAGCAAAGAAAGGTTTAGGCAGCGGCTTTGGTGCTTTCTTTTTGAGAATCTTAACAGGGCTGTGGATGAACTTTATCTTCTTTGCGAACTAGAATGCGATGTAGAGCAGATGAAAGAGGCAATTCTTGTTCTTGAAGAGGCTGCCTCTGATTTTCGAGATCTAAGCACTAGAGTGGAAGACTTTGAGGAAATAAAAAGGTCGTCATCCCAGATAATTGATGGTGTGCCCATTACCTTGAAGAGCGACCACCGTAGGCCGCATGCTCTCTCATGGGAGGTTAGAAGAATGACAACTTCAGCACACAAAGCAGAAATACTATCGTCATCTCTTGAAGCGTTTAAGAAAATTCAACAAGAAAGAGCTAGCTTGTGTGCAACTACCGATGATAAACTTTTGGGGCCTCAGTACCTAAATCTCCGATGTGTTGATAAGCTGAATAAGTTATCTGCCATAAATGATGGGAAATTTAATACTAAAGATTCAATAAAGAAGTCAGGAAAGCAGAATGGAGGTTCGGATCTTGCTGAAGCTGACATAAATGGAGGAAAAGGCAATACTGAGTCAAGCTGTTCGAGCAAAACAAACTTGGTACAAAGTGCACGTGCCCCTCATAATGCATCTTCCTCTGTTGTTAATGCATCTAGGCTGCCTCCTAGGGATAGTTCTGCTGCTGGAAAGACTAAGAGTAAACAGTTTGGATCCGAAGCCGAGAGGCTGCTCCCTAAGAAAGATAAATTGTCAACAGAAGGTGGTGTTGAAAAATTCCCCAGATTGACGGATCAATCCAAAAAGGAAATTCCTCTATTTGAGAAAGATAAAGAAAAGAGGAACTCAGCTCCATGGAAATCTATGGATGCTTGGAAAGAGAAGAGGAACTGGGAGAACGTACTTTCCTCTCCTTCCCGTGTCTCTTCTCGTGTTTCACGTTCACCTGGAATGAGGAGGAAAAGTGCTGACCGTGCACGTATGCTGCATTATAAACTAATGTCTCCTGAGAAGAAGAAGAAAACTGCTCTTGATCTGAAGAGAGAAGCAGAAGAAAAGCATGCTCGTGCTCTGAGAATTAAAAGTGAGCTAGATAATGAGAGAGCTCAAAAACTTCATCGCAATTCAGAAAAGGTGTATCGAGCTAGTGCATTTCACGTTGTTCGCAACATGAAGTTACGTGAGGGAATATATGCTCGTCATCAACGCAGTGAGTCTCGCCACGAAGCTTTTCTGGCTCAAGTTGTAAAGAGAGCTGGTGATGAAAGCAGTAAAGTTAATGAGGTTCGTTTCATAACTTCCTTAAATGAAGAAAATAAAAAACTTAGTTTGCGCCAGAAGCTTCATGATTCAGAGTTGAGGAGAGCTGAAAAACTTCAAGTGATAAGAACTAAACAGAAAGAGGATATGGCAAGAGAAGAAGCTGTTTTAGAACGGAGGAAACTCATTGAAGCTGAAAAGTTGCAGCGTCTTGCTGAGACACAGAAAAGAAAGGAAGAGGCTCTAGTTAGAAGAGAAGAGGAACGCAAAGCGTCTAGTGCAGCCCGTGAAGCAAGGGCCATGGAACAACTTCGTAGGAAGGAGGAAAGAGCCAAAGCTCAGCAGGAAGAGGCTGAACTTCTGGCCCAGAAATTGGCTGAGAGACTTAGTGAAAGTGAGCAACGCCGAAAGTTTTACCTGGAGCAGATACGAGAGAGAGCTTCTATGGATTTCAGGGATCAATCTTCGCCTTTATTCCGCCGAACCCTGAACAAGGAGGGTCAGGGAAGATCTTCATCGGTCAACAATGGTGATGAGTATCAAGGGGCCAACTTCTCAGGCTTAGGAGGTTCTACTCTTGTGGCAAGCAATGTTACAGCACAACATTCAATGAAGCGGAGAATCAAAAGAATTCGGCAAAGACTAATGGCTCTAAAATATGATTTTCCCGAGCCTCCTGCTGGTGCTGAAAATGCTGGCATTGGATACAGAACGGCAGTGGGAACTGCAAGGGCAAAGATTGGAAGATGGCTTCAAGAACTTAAAACACTCCGGCAGGCAAGAAAAGAAGGGGCTGCAAGTATTGGGTTAATAATTGCTGAAATGATCAAGTATTTGGAGGGAAAGGAGCCTGAACTGCAGGCTTCCCGCCAAGCTGGTTTGCTTGATTTCATAGCTTCTACTTTACCTGCTTCTCATACATCAAAACCTGAAGCCTGCCAAGTGACAATACACCTTTTAAAGCTTTTGAGGGTGGTGTTATCAGTGCCTGCAAACAGGAGTTATTTTCTCATACAGAATCTCTTACCTCCTATCATCCCGATGCTGGCAGCAGCCCTCGAGAGCTACATAAAGATGGCAGTATCCTTGAACCTTTCGGCTAATGTTAATTCCCTATCAAGCAAAACCTCAGCTGAAAATTTTGAATCGATGTCTGAGGTTCTGGATGGTTATTTATGGACTGTGACAACAATCATCAGTCATATAAGTTCTGATGAACGACAGCTCCAAATGCGGGATGGCTTGCTTGAGCTGTTGATTGCCTACCAAGTTATTCAACGGCTGCGAGATCTCTTTGCACTTTATGATCGGCCCCAGGTGGAAGGGTCTCCATTTCCATCTTCAATTCTCTTAAGTATAAATTTGTTGGTCGTTTTAACTTCCAGATCTGAAATCAATGGCTCTATCAATTGGGAATCTGTGCCTATTGAAACAATGGTAGGAAATGGATGTGAAGAGGCTAAATTTGCGGAGCGTGACAGCATTGAATTTCTTCCTATCACTCAGTCCTTGGGAGATTCTAGACCTCCATTCTCTTTCGTCAATGGTGGGAAAGCTGTACATCTACTAGATGTACCCGAGGAAAGTCCATTAAATGAATCATGTCCGATAAATAAGTCTGCTGAGTCAGTATCCGCCATTAAGGATGGTGAGAAAGAGCAGTCTAGTAGTTTGGTAGCAGGCAGGAATGCTAACACAATCAGCACAGATGTTCCAGATGAACCCCAGAAGATTCCAGTAATGGATATTATGGAGCCTTTAGCATCACAAAGGGATGAGAAACCCTTGGTAGTTAACGGTGCAGAACAAAAGAATGAAAACATTGTGAGTGCAGAGCAACCGGTAGCGTTTCTTCTTTCTGCTGTATCAGAGACTGGGCTTGTCAGCCTCCCTTCTCTGTTGACATCTGTCCTACTGCAGGCAAACAATAGATTGGCTTCTGAACAGACCTCAGATGCCCTTCCCTCTAATTTTGAAGACGTGGCAACTGGAGTACTGAAGGTGGGGTTTCTTCTACTGGAATCTCTTTTGCTCCTTGGCCACTTTGCGTTGTTCCATCTCGGAAATCAGGCTGTCCTTCGCTGGGGAAAGAGTCCTACCATCATTCACAAGGTATGTGATTTACCGTTCGTATTCTTCAGTGACCCTGAGTTGATGCCAATCTTGGCTGGTACACTGGTTGCTGCCTGTTATGGGTGTGAGCAGAACAAGGGTGTTGTTCAACAGGAAATCAGTACGGATATGTTACTCTCATTGTTACGATCTTGTAGAAATGTCTTGCCGTCCGTTAAATCGAATTCAAACTTGGACAATTCCCAAGCAGATAGTGTTCCCCTAAGATCCGGGCGCAACAATGCCAAGAGCACTATAAGCACTAGGCTTTCTTCAGTGAAGGGTGGTGCTTCAGGAAACAGCTTGAGAATTGGCGGCAAGATGAAAAGCCATAGGGAAAGTAAAGCAACAAAGAGTCCCGGAGAGACGGCTGAGAAGGATAATCTTCCAGTTTCGGACACTTCCTCCGTGATGTTGCACTCTAGATTTCCTATCAGCTTCATCGATAGAGCAGAAGACTTCTTTTCCACTGGGACCCCTGGCGTCGATGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAPER_N

Protein Analysis

1645

Amino Acids

181.73

Weight (kDa)

6.82

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAPER_N PF16501 359 - 460 2.6e-15 S phase cyclin A-associated protein in the endoplasmic reticulum
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2058
Acc36I ACCTGC 1 cut(s) 3241
AccB1I GGYRCC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 2698
AccI GTMKAC 1 cut(s) 971
AciI CCGC 9 cut(s) 1102, 1337, 2803, 2941, 3193, 3559, 3794, 3960, 4752
AcoI YGGCCR 1 cut(s) 4363
AcvI CACGTG 1 cut(s) 1682
AcyI GRCGYC 1 cut(s) 4920
AdeI CACNNNGTG 1 cut(s) 1013
AflII CTTAAG 1 cut(s) 3676
AflIII ACRYGT 1 cut(s) 751
AgeI ACCGGT 1 cut(s) 4165
AjiI CACGTC 1 cut(s) 4306
AjnI CCWGG 4 cut(s) 2011, 2742, 3639, 4915
AjuI GAANNNNNNNTTGG 2 cut(s) 4613, 4645
AleI CACNNNNGTG 1 cut(s) 1250
AloI GAACNNNNNNTCC 4 cut(s) 1955, 1987, 3534, 3566
Alw21I GWGCWC 6 cut(s) 289, 1681, 2044, 2137, 2173, 4691
Alw26I GTCTC 9 cut(s) 535, 1993, 2073, 2286, 2537, 2701, 3657, 4190, 4796
Alw44I GTGCAC 2 cut(s) 1677, 2040
AlwNI CAGNNNCTG 3 cut(s) 605, 3314, 4199
Ama87I CYCGRG 6 cut(s) 177, 443, 532, 2993, 3383, 3902
AoxI GGCC 7 cut(s) 1334, 1478, 2622, 2688, 2871, 3635, 4363
ApaLI GTGCAC 2 cut(s) 1677, 2040
AsiGI ACCGGT 1 cut(s) 4165
Asp700I GAANNNNTTC 5 cut(s) 2295, 2439, 2634, 2836, 3816
AspA2I CCTAGG 1 cut(s) 1733
AspLEI GCGC 3 cut(s) 189, 2400, 4674
AspS9I GGNCC 8 cut(s) 147, 994, 1478, 2622, 2689, 2871, 3636, 4911
AsuC2I CCSGG 5 cut(s) 213, 236, 282, 4669, 4797
AsuHPI GGTGA 8 cut(s) 461, 661, 812, 869, 2001, 2336, 2867, 3986
AsuII TTCGAA 2 cut(s) 48, 725
AsuNHI GCTAGC 1 cut(s) 1442
AvaI CYCGRG 6 cut(s) 177, 443, 532, 2993, 3383, 3902
AvaII GGWCC 3 cut(s) 147, 994, 4911
AvrII CCTAGG 1 cut(s) 1733
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 5 cut(s) 1057, 1308, 1681, 1687, 2044
BaeI ACNNNNGTAYC 2 cut(s) 4488, 4521
BalI TGGCCA 1 cut(s) 4365
BamHI GGATCC 1 cut(s) 1778
BanI GGYRCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 38, 2173
BarI GAAGNNNNNNTAC 2 cut(s) 4161, 4193
BauI CACGAG 2 cut(s) 1995, 2130
BbrPI CACGTG 1 cut(s) 1682
BbsI GAAGAC 3 cut(s) 1263, 4308, 4899
Bbv12I GWGCWC 6 cut(s) 289, 1681, 2044, 2137, 2173, 4691
BbvCI CCTCAGC 1 cut(s) 3454
BceAI ACGGC 4 cut(s) 3054, 3620, 4591, 4821
BcgI CGANNNNNNTGC 4 cut(s) 401, 435, 2984, 3018
BciT130I CCWGG 4 cut(s) 2013, 2744, 3641, 4917
BciVI GTATCC 3 cut(s) 3023, 3418, 3967
BclI TGATCA 1 cut(s) 3150
BcnI CCSGG 5 cut(s) 213, 236, 282, 4669, 4797
BcoDI GTCTC 9 cut(s) 535, 1993, 2073, 2286, 2537, 2701, 3657, 4190, 4796
BfmI CTRYAG 4 cut(s) 89, 375, 3182, 4241
BfrI CTTAAG 1 cut(s) 3676
BfuAI ACCTGC 1 cut(s) 3241
BfuI GTATCC 3 cut(s) 3023, 3418, 3967
BglII AGATCT 4 cut(s) 1237, 2834, 3613, 3710
BlnI CCTAGG 1 cut(s) 1733
BlpI GCTNAGC 1 cut(s) 2664
BmcAI AGTACT 2 cut(s) 721, 4320
Bme1390I CCNGG 9 cut(s) 213, 236, 282, 2013, 2744, 3641, 4669, 4797, 4917
Bme18I GGWCC 3 cut(s) 147, 994, 4911
BmeT110I CYCGRG 6 cut(s) 177, 443, 532, 2993, 3383, 3902
BmgBI CACGTC 1 cut(s) 4306
BmgT120I GGNCC 8 cut(s) 147, 994, 1478, 2622, 2689, 2871, 3636, 4911
BmiI GGNNCC 9 cut(s) 487, 1479, 1780, 2872, 3174, 3638, 4080, 4912, 4913
BmrFI CCNGG 9 cut(s) 213, 236, 282, 2013, 2744, 3641, 4669, 4797, 4917
BmrI ACTGGG 4 cut(s) 900, 1966, 4209, 4917
BmtI GCTAGC 1 cut(s) 1446
BmuI ACTGGG 4 cut(s) 900, 1966, 4209, 4917
BoxI GACNNNNGTC 1 cut(s) 4232
BpiI GAAGAC 3 cut(s) 1263, 4308, 4899
BplI GAGNNNNNCTC 2 cut(s) 1208, 1240
BpmI CTGGAG 2 cut(s) 2765, 4335
Bpu10I CCTNAGC 3 cut(s) 333, 2887, 3454
Bpu1102I GCTNAGC 1 cut(s) 2664
Bpu14I TTCGAA 2 cut(s) 48, 725
BpuEI CTTGAG 3 cut(s) 2289, 3593, 4762
BpuMI CCSGG 5 cut(s) 213, 236, 282, 4669, 4797
Bsa29I ATCGAT 2 cut(s) 3473, 4881
BsaAI YACGTR 3 cut(s) 1682, 2045, 2246
BsaBI GATNNNNATC 4 cut(s) 519, 2839, 2862, 4484
BsaHI GRCGYC 1 cut(s) 4920
BsaI GGTCTC 2 cut(s) 535, 3657
BsaWI WCCGGW 1 cut(s) 4165
Bse118I RCCGGY 1 cut(s) 4165
Bse21I CCTNAGG 1 cut(s) 849
Bse3DI GCAATG 1 cut(s) 2920
Bse8I GATNNNNATC 4 cut(s) 519, 2839, 2862, 4484
BseBI CCWGG 4 cut(s) 2013, 2744, 3641, 4917
BseCI ATCGAT 2 cut(s) 3473, 4881
BseJI GATNNNNATC 4 cut(s) 519, 2839, 2862, 4484
BseMI GCAATG 1 cut(s) 2920
BseRI GAGGAG 2 cut(s) 2035, 2440
BseSI GKGCMC 5 cut(s) 1057, 1308, 1681, 1687, 2044
BseYI CCCAGC 2 cut(s) 195, 4407
BsgI GTGCAG 3 cut(s) 2625, 4146, 4176
BshFI GGCC 7 cut(s) 1336, 1480, 2624, 2690, 2873, 3637, 4365
BshNI GGYRCC 1 cut(s) 485
BshTI ACCGGT 1 cut(s) 4165
BshVI ATCGAT 2 cut(s) 3473, 4881
BsiHKAI GWGCWC 6 cut(s) 289, 1681, 2044, 2137, 2173, 4691
BsiHKCI CYCGRG 6 cut(s) 177, 443, 532, 2993, 3383, 3902
BsiSI CCGG 7 cut(s) 212, 235, 281, 3097, 4166, 4668, 4797
BslFI GGGAC 4 cut(s) 194, 248, 4779, 4924
BsmAI GTCTC 9 cut(s) 535, 1993, 2073, 2286, 2537, 2701, 3657, 4190, 4796
BsmBI CGTCTC 1 cut(s) 4796
BsmFI GGGAC 4 cut(s) 194, 248, 4779, 4924
BsmI GAATGC 2 cut(s) 1178, 4018
BsnI GGCC 7 cut(s) 1336, 1480, 2624, 2690, 2873, 3637, 4365
Bso31I GGTCTC 2 cut(s) 535, 3657
BsoBI CYCGRG 6 cut(s) 177, 443, 532, 2993, 3383, 3902
Bsp119I TTCGAA 2 cut(s) 48, 725
Bsp1407I TGTACA 1 cut(s) 3883
Bsp1720I GCTNAGC 1 cut(s) 2664
Bsp19I CCATGG 2 cut(s) 1919, 2625
BspACI CCGC 9 cut(s) 1102, 1337, 2803, 2941, 3193, 3559, 3794, 3960, 4752
BspANI GGCC 7 cut(s) 1336, 1480, 2624, 2690, 2873, 3637, 4365
BspDI ATCGAT 2 cut(s) 3473, 4881
BspHI TCATGA 2 cut(s) 295, 2410
BspLI GGNNCC 9 cut(s) 487, 1479, 1780, 2872, 3174, 3638, 4080, 4912, 4913
BspMAI CTGCAG 2 cut(s) 3186, 4245
BspMI ACCTGC 1 cut(s) 3241
BspOI GCTAGC 1 cut(s) 1446
BspQI GCTCTTC 2 cut(s) 672, 1313
BspT104I TTCGAA 2 cut(s) 48, 725
BspT107I GGYRCC 1 cut(s) 485
BspTI CTTAAG 1 cut(s) 3676
BspTNI GGTCTC 2 cut(s) 535, 3657
BsrDI GCAATG 1 cut(s) 2920
BsrFI RCCGGY 1 cut(s) 4165
BsrGI TGTACA 1 cut(s) 3883
BssAI RCCGGY 1 cut(s) 4165
BssNI GRCGYC 1 cut(s) 4920
BssSI CACGAG 2 cut(s) 1995, 2130
BssT1I CCWWGG 7 cut(s) 1057, 1733, 1919, 2625, 3834, 4110, 4360
Bst2BI CACGAG 2 cut(s) 1995, 2130
Bst2UI CCWGG 4 cut(s) 2013, 2744, 3641, 4917
Bst4CI ACNGT 9 cut(s) 11, 719, 817, 1331, 1773, 2039, 3508, 4124, 4452
BstACI GRCGYC 1 cut(s) 4920
BstAFI CTTAAG 1 cut(s) 3676
BstAUI TGTACA 1 cut(s) 3883
BstBAI YACGTR 3 cut(s) 1682, 2045, 2246
BstBI TTCGAA 2 cut(s) 48, 725
BstDSI CCRYGG 2 cut(s) 1919, 2625
BstEII GGTNACC 1 cut(s) 857
BstENI CCTNNNNNAGG 3 cut(s) 852, 2816, 4241
BstHHI GCGC 3 cut(s) 189, 2400, 4674
BstMAI GTCTC 9 cut(s) 535, 1993, 2073, 2286, 2537, 2701, 3657, 4190, 4796
BstNI CCWGG 4 cut(s) 2013, 2744, 3641, 4917
BstNSI RCATGY 2 cut(s) 1343, 2129
BstPAI GACNNNNGTC 1 cut(s) 4232
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 9 cut(s) 211, 234, 280, 2011, 2742, 3639, 4667, 4795, 4915
BstSFI CTRYAG 4 cut(s) 89, 375, 3182, 4241
BstSLI GKGCMC 5 cut(s) 1057, 1308, 1681, 1687, 2044
BstV2I GAAGAC 3 cut(s) 1263, 4308, 4899
BstX2I RGATCY 9 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3710, 4664
BstYI RGATCY 9 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3710, 4664
Bsu15I ATCGAT 2 cut(s) 3473, 4881
Bsu36I CCTNAGG 1 cut(s) 849
BsuI GTATCC 3 cut(s) 3023, 3418, 3967
BsuRI GGCC 7 cut(s) 1336, 1480, 2624, 2690, 2873, 3637, 4365
BsuTUI ATCGAT 2 cut(s) 3473, 4881
BtgI CCRYGG 2 cut(s) 1919, 2625
BtgZI GCGATG 2 cut(s) 1191, 2168
BtrI CACGTC 1 cut(s) 4306
BtsI GCAGTG 3 cut(s) 172, 2281, 3048
BveI ACCTGC 1 cut(s) 3241
CaiI CAGNNNCTG 3 cut(s) 605, 3314, 4199
CciI TCATGA 2 cut(s) 295, 2410
CfoI GCGC 3 cut(s) 189, 2400, 4674
Cfr10I RCCGGY 1 cut(s) 4165
Cfr13I GGNCC 8 cut(s) 147, 994, 1478, 2622, 2689, 2871, 3636, 4911
ClaI ATCGAT 2 cut(s) 3473, 4881
CseI GACGC 3 cut(s) 2522, 2586, 4909
CspAI ACCGGT 1 cut(s) 4165
DraI TTTAAA 1 cut(s) 3285
DraIII CACNNNGTG 1 cut(s) 1013
EaeI YGGCCR 1 cut(s) 4363
EciI GGCGGA 2 cut(s) 2792, 3949
Ecl136II GAGCTC 1 cut(s) 2171
Eco130I CCWWGG 7 cut(s) 1057, 1733, 1919, 2625, 3834, 4110, 4360
Eco24I GRGCYC 2 cut(s) 38, 2173
Eco31I GGTCTC 2 cut(s) 535, 3657
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 3 cut(s) 147, 994, 4911
Eco53kI GAGCTC 1 cut(s) 2171
Eco72I CACGTG 1 cut(s) 1682
Eco81I CCTNAGG 1 cut(s) 849
Eco88I CYCGRG 6 cut(s) 177, 443, 532, 2993, 3383, 3902
Eco91I GGTNACC 1 cut(s) 857
EcoICRI GAGCTC 1 cut(s) 2171
EcoNI CCTNNNNNAGG 3 cut(s) 852, 2816, 4241
EcoO109I RGGNCCY 2 cut(s) 1478, 4911
EcoO65I GGTNACC 1 cut(s) 857
EcoRI GAATTC 2 cut(s) 2955, 4621
EcoRII CCWGG 4 cut(s) 2011, 2742, 3639, 4915
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 7 cut(s) 1057, 1733, 1919, 2625, 3834, 4110, 4360
EcoT22I ATGCAT 2 cut(s) 1699, 1720
EcoT38I GRGCYC 2 cut(s) 38, 2173
ErhI CCWWGG 7 cut(s) 1057, 1733, 1919, 2625, 3834, 4110, 4360
Esp3I CGTCTC 1 cut(s) 4796
FaqI GGGAC 4 cut(s) 194, 248, 4779, 4924
FauI CCCGC 2 cut(s) 3200, 3552
FbaI TGATCA 1 cut(s) 3150
FblI GTMKAC 1 cut(s) 971
FriOI GRGCYC 2 cut(s) 38, 2173
GlaI GCGC 3 cut(s) 188, 2399, 4673
GsaI CCCAGC 2 cut(s) 199, 4411
GsuI CTGGAG 2 cut(s) 2765, 4335
HaeIII GGCC 7 cut(s) 1336, 1480, 2624, 2690, 2873, 3637, 4365
HapII CCGG 7 cut(s) 212, 235, 281, 3097, 4166, 4668, 4797
HgaI GACGC 3 cut(s) 2522, 2586, 4909
HhaI GCGC 3 cut(s) 189, 2400, 4674
Hin1I GRCGYC 1 cut(s) 4920
Hin6I GCGC 3 cut(s) 187, 2398, 4672
HinP1I GCGC 3 cut(s) 187, 2398, 4672
HincII GTYRAC 4 cut(s) 1821, 2848, 4120, 4227
HindII GTYRAC 4 cut(s) 1821, 2848, 4120, 4227
HindIII AAGCTT 4 cut(s) 701, 2292, 2405, 3287
HpaI GTTAAC 1 cut(s) 4120
HpaII CCGG 7 cut(s) 212, 235, 281, 3097, 4166, 4668, 4797
HphI GGTGA 8 cut(s) 461, 661, 812, 869, 2001, 2336, 2867, 3986
Hpy99I CGWCG 1 cut(s) 4925
HpyCH4III ACNGT 9 cut(s) 11, 719, 817, 1331, 1773, 2039, 3508, 4124, 4452
HpyCH4IV ACGT 8 cut(s) 753, 1681, 1965, 2005, 2044, 2223, 2245, 4305
HpySE526I ACGT 8 cut(s) 753, 1681, 1965, 2005, 2044, 2223, 2245, 4305
Hsp92I GRCGYC 1 cut(s) 4920
HspAI GCGC 3 cut(s) 187, 2398, 4672
KflI GGGWCCC 1 cut(s) 4911
Ksp22I TGATCA 1 cut(s) 3150
KspAI GTTAAC 1 cut(s) 4120
LguI GCTCTTC 2 cut(s) 672, 1313
LmnI GCTCC 9 cut(s) 292, 1803, 1921, 2746, 3172, 3555, 3796, 4078, 4362
MaeII ACGT 8 cut(s) 753, 1681, 1965, 2005, 2044, 2223, 2245, 4305
MfeI CAATTG 2 cut(s) 900, 3733
MflI RGATCY 9 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3710, 4664
MlsI TGGCCA 1 cut(s) 4365
MluNI TGGCCA 1 cut(s) 4365
MlyI GAGTC 5 cut(s) 1649, 2288, 3959, 4426, 4801
MmeI TCCRAC 1 cut(s) 125
Mox20I TGGCCA 1 cut(s) 4365
Mph1103I ATGCAT 2 cut(s) 1699, 1720
MroXI GAANNNNTTC 5 cut(s) 2295, 2439, 2634, 2836, 3816
MscI TGGCCA 1 cut(s) 4365
MslI CAYNNNNRTG 5 cut(s) 546, 1250, 2130, 3365, 3398
Msp20I TGGCCA 1 cut(s) 4365
MspA1I CMGCKG 2 cut(s) 1102, 3458
MspCI CTTAAG 1 cut(s) 3676
MspI CCGG 7 cut(s) 212, 235, 281, 3097, 4166, 4668, 4797
MspR9I CCNGG 9 cut(s) 213, 236, 282, 2013, 2744, 3641, 4669, 4797, 4917
MunI CAATTG 2 cut(s) 900, 3733
Mva1269I GAATGC 2 cut(s) 1178, 4018
MvaI CCWGG 4 cut(s) 2013, 2744, 3641, 4917
NciI CCSGG 5 cut(s) 213, 236, 282, 4669, 4797
NcoI CCATGG 2 cut(s) 1919, 2625
NheI GCTAGC 1 cut(s) 1442
NlaIV GGNNCC 9 cut(s) 487, 1479, 1780, 2872, 3174, 3638, 4080, 4912, 4913
NmeAIII GCCGAG 1 cut(s) 1813
NmuCI GTSAC 6 cut(s) 857, 1067, 3268, 3508, 3800, 4466
NsiI ATGCAT 2 cut(s) 1699, 1720
NspI RCATGY 2 cut(s) 1343, 2129
NspV TTCGAA 2 cut(s) 48, 725
OliI CACNNNNGTG 1 cut(s) 1250
PaeI GCATGC 2 cut(s) 1343, 2129
PaeR7I CTCGAG 3 cut(s) 443, 532, 3383
PagI TCATGA 2 cut(s) 295, 2410
PciSI GCTCTTC 2 cut(s) 672, 1313
PcsI WCGNNNNNNNCGW 2 cut(s) 408, 2002
PctI GAATGC 2 cut(s) 1178, 4018
PdmI GAANNNNTTC 5 cut(s) 2295, 2439, 2634, 2836, 3816
PflMI CCANNNNNTGG 1 cut(s) 2698
PfoI TCCNGGA 1 cut(s) 4795
PinAI ACCGGT 1 cut(s) 4165
PleI GAGTC 5 cut(s) 1648, 2287, 3958, 4425, 4800
PmaCI CACGTG 1 cut(s) 1682
PmlI CACGTG 1 cut(s) 1682
PpsI GAGTC 5 cut(s) 1648, 2287, 3958, 4425, 4800
Ppu21I YACGTR 3 cut(s) 1682, 2045, 2246
PpuMI RGGWCCY 1 cut(s) 4911
PshAI GACNNNNGTC 1 cut(s) 4232
PsiI TTATAA 1 cut(s) 2058
Psp124BI GAGCTC 1 cut(s) 2173
Psp5II RGGWCCY 1 cut(s) 4911
Psp6I CCWGG 4 cut(s) 2011, 2742, 3639, 4915
PspCI CACGTG 1 cut(s) 1682
PspEI GGTNACC 1 cut(s) 857
PspFI CCCAGC 2 cut(s) 195, 4407
PspGI CCWGG 4 cut(s) 2011, 2742, 3639, 4915
PspN4I GGNNCC 9 cut(s) 487, 1479, 1780, 2872, 3174, 3638, 4080, 4912, 4913
PspPI GGNCC 8 cut(s) 147, 994, 1478, 2622, 2689, 2871, 3636, 4911
PspPPI RGGWCCY 1 cut(s) 4911
PstI CTGCAG 2 cut(s) 3186, 4245
PstNI CAGNNNCTG 3 cut(s) 605, 3314, 4199
PsuI RGATCY 9 cut(s) 191, 439, 1237, 1597, 1778, 2834, 3613, 3710, 4664
PvuII CAGCTG 1 cut(s) 3458
RseI CAYNNNNRTG 5 cut(s) 546, 1250, 2130, 3365, 3398
SacI GAGCTC 1 cut(s) 2173
SapI GCTCTTC 2 cut(s) 672, 1313
Sau96I GGNCC 8 cut(s) 147, 994, 1478, 2622, 2689, 2871, 3636, 4911
ScaI AGTACT 2 cut(s) 721, 4320
SchI GAGTC 5 cut(s) 1649, 2288, 3959, 4426, 4801
ScrFI CCNGG 9 cut(s) 213, 236, 282, 2013, 2744, 3641, 4669, 4797, 4917
SfcI CTRYAG 4 cut(s) 89, 375, 3182, 4241
Sfr274I CTCGAG 3 cut(s) 443, 532, 3383
SfuI TTCGAA 2 cut(s) 48, 725
SinI GGWCC 3 cut(s) 147, 994, 4911
SlaI CTCGAG 3 cut(s) 443, 532, 3383
SmiMI CAYNNNNRTG 5 cut(s) 546, 1250, 2130, 3365, 3398
SmlI CTYRAG 7 cut(s) 443, 532, 2304, 3383, 3572, 3676, 4741
SmoI CTYRAG 7 cut(s) 443, 532, 2304, 3383, 3572, 3676, 4741
SphI GCATGC 2 cut(s) 1343, 2129
SsiI CCGC 9 cut(s) 1102, 1337, 2803, 2941, 3193, 3559, 3794, 3960, 4752
SstI GAGCTC 1 cut(s) 2173
StyD4I CCNGG 9 cut(s) 211, 234, 280, 2011, 2742, 3639, 4667, 4795, 4915
StyI CCWWGG 7 cut(s) 1057, 1733, 1919, 2625, 3834, 4110, 4360
TaaI ACNGT 9 cut(s) 11, 719, 817, 1331, 1773, 2039, 3508, 4124, 4452
TaiI ACGT 8 cut(s) 756, 1684, 1968, 2008, 2047, 2226, 2248, 4308
TaqII GACCGA 1 cut(s) 2833
TatI WGTACW 3 cut(s) 719, 3883, 4318
TauI GCSGC 3 cut(s) 1105, 1339, 4755
TseFI GTSAC 6 cut(s) 857, 1067, 3268, 3508, 3800, 4466
Tsp45I GTSAC 6 cut(s) 857, 1067, 3268, 3508, 3800, 4466
TspGWI ACGGA 5 cut(s) 1871, 2515, 4577, 4600, 4834
Van91I CCANNNNNTGG 1 cut(s) 2698
Vha464I CTTAAG 1 cut(s) 3676
VneI GTGCAC 2 cut(s) 1677, 2040
VpaK11BI GGWCC 3 cut(s) 147, 994, 4911
XagI CCTNNNNNAGG 3 cut(s) 852, 2816, 4241
XbaI TCTAGA 2 cut(s) 3845, 4859
XceI RCATGY 2 cut(s) 1343, 2129
XcmI CCANNNNNNNNNTGG 2 cut(s) 1295, 3560
XhoI CTCGAG 3 cut(s) 443, 532, 3383
XmaJI CCTAGG 1 cut(s) 1733
XmiI GTMKAC 1 cut(s) 971
XmnI GAANNNNTTC 5 cut(s) 2295, 2439, 2634, 2836, 3816
ZrmI AGTACT 2 cut(s) 721, 4320
Zsp2I ATGCAT 2 cut(s) 1699, 1720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.