pycom14g17300

Plant intracellular Ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005398
Physical Location & Seq
Reverse (-)
19295317 .. 19298063
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGGGTTGCAATACAAGCAGAAACGTGGATTCGAAAGCAAGTCGGGCCGCCCGGTGGCGAACCACCGGCATTGTTGGCCTCCGTGATTCCAAATTGAAGACATTTCCAGATGAAGTTCTTGACCTGGACAGGTCCATACGTACTCTTGATCTAACACACAATAAAATAGTTGACATTCCTGTGGATATCAGCAAATTAGTAAACCTGCAGCGACTGATATTAGCTCATAACCTCATTGACCGACTGCCGATAAATCTGGCAAAACTAGTGTCCTTAAAAGTTATGACACTTGATGGAAATCGAGTTACTACGTTGCCTGATGAATTGGGACAGTTGGTGAGACTTGAGCAGTTATCTATCTCTGGGAATGCATTAGAGTCTTTGCCTGAGACTATTGGAAGCTTGCGGAATTTAAAGCTTTTAAATTTATCAAAGAACAAATTAAAGTCTCTTCCTGAATCCATCGGAAGTTGCTTCTCTCTGGAAGAATTACAAGCAAATGATAATTCAATTGAAGAACTTCCTGAATCAGTTTGCAACCTTACCCACGTAAAGTCACTTTGCTTGGACAATAATAGTGTGAAGCAGATACCCACTAATCTGTTGAAGGACTGTAAAGCGCTGCAGAATATTTCCCTTCATGGAAACCCTATTTCCATGGATCAGTTTCAGCAGATGGAAGGATTCCAAGAGTTTGAAGCGAGGAGGAAGAAGAAGTTCGACAAACAAATTGACTCAAATGTGATGATCAGTTCGAAAGGCCTTGACGAGGGTGTTGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8

Protein Analysis

262

Amino Acids

29.12

Weight (kDa)

6.91

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 26 - 79 7.1e-06 Leucine rich repeat
LRR_8 PF13855 45 - 101 3.6e-09 Leucine rich repeat
LRR_8 PF13855 68 - 125 8.7e-08 Leucine rich repeat
LRR_14 PF23598 85 - 165 6.5e-13 Leucine-rich repeat region
LRR_8 PF13855 114 - 171 2.3e-07 Leucine rich repeat
LRR_14 PF23598 131 - 245 4.4e-13 Leucine-rich repeat region
LRR_8 PF13855 160 - 218 7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 213
AciI CCGC 2 cut(s) 48, 406
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 2 cut(s) 409, 424
AfaI GTAC 1 cut(s) 142
AfeI AGCGCT 1 cut(s) 621
AfiI CCNNNNNNNGG 2 cut(s) 54, 769
AgsI TTSAA 5 cut(s) 97, 510, 515, 607, 698
AhlI ACTAGT 1 cut(s) 265
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 3 cut(s) 224, 402, 418
AluI AGCT 3 cut(s) 224, 402, 418
Alw26I GTCTC 3 cut(s) 334, 383, 453
AlwI GGATC 1 cut(s) 669
AlwNI CAGNNNCTG 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 621
AoxI GGCC 3 cut(s) 45, 76, 760
ApeKI GCWGC 2 cut(s) 208, 622
ApoI RAATTY 2 cut(s) 409, 424
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
Asp700I GAANNNNTTC 2 cut(s) 519, 716
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 2 cut(s) 45, 132
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 349
AsuII TTCGAA 2 cut(s) 32, 755
AvaII GGWCC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 220, 609
BccI CCATC 3 cut(s) 287, 470, 670
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 747
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 334, 383, 453
BcuI ACTAGT 1 cut(s) 265
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 2 cut(s) 206, 623
BfoI RGCGCY 1 cut(s) 623
BfuAI ACCTGC 1 cut(s) 213
BisI GCNGC 3 cut(s) 48, 209, 623
BlsI GCNGC 3 cut(s) 49, 210, 624
Bme1390I CCNGG 2 cut(s) 52, 125
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 45, 132
BmrFI CCNGG 2 cut(s) 52, 125
BpiI GAAGAC 1 cut(s) 104
Bpu14I TTCGAA 2 cut(s) 32, 755
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 52
BsaAI YACGTR 2 cut(s) 140, 550
BsaBI GATNNNNATC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 657
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 769
Bse118I RCCGGY 1 cut(s) 65
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 657
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 54, 769
BseMII CTCAG 1 cut(s) 378
BseRI GAGGAG 1 cut(s) 718
BseXI GCAGC 2 cut(s) 220, 609
BshFI GGCC 3 cut(s) 47, 78, 762
BsiSI CCGG 2 cut(s) 52, 66
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 54, 769
BsmAI GTCTC 3 cut(s) 334, 383, 453
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 3 cut(s) 47, 78, 762
Bsp119I TTCGAA 2 cut(s) 32, 755
Bsp143I GATC 4 cut(s) 148, 661, 747, 778
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 2 cut(s) 48, 406
BspANI GGCC 3 cut(s) 47, 78, 762
BspCNI CTCAG 1 cut(s) 379
BspMAI CTGCAG 2 cut(s) 210, 627
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 1 cut(s) 669
BspT104I TTCGAA 2 cut(s) 32, 755
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 4 cut(s) 148, 661, 747, 778
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 333, 614
Bst6I CTCTTC 1 cut(s) 456
BstBAI YACGTR 2 cut(s) 140, 550
BstBI TTCGAA 2 cut(s) 32, 755
BstC8I GCNNGC 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 657
BstENI CCTNNNNNAGG 1 cut(s) 767
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 4 cut(s) 151, 664, 750, 781
BstMAI GTCTC 3 cut(s) 334, 383, 453
BstMBI GATC 4 cut(s) 148, 661, 747, 778
BstMWI GCNNNNNNNGC 3 cut(s) 15, 44, 75
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 50, 123
BstSFI CTRYAG 2 cut(s) 206, 623
BstSNI TACGTA 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 220, 609
BstV2I GAAGAC 1 cut(s) 104
BsuRI GGCC 3 cut(s) 47, 78, 762
BtgI CCRYGG 1 cut(s) 657
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 404
CaiI CAGNNNCTG 1 cut(s) 214
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 45, 132
Csp6I GTAC 1 cut(s) 141
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 2 cut(s) 641, 658
CviJI RGCY 6 cut(s) 47, 78, 224, 402, 418, 762
CviKI_1 RGCY 6 cut(s) 47, 78, 224, 402, 418, 762
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 1 cut(s) 387
DpnI GATC 4 cut(s) 150, 663, 749, 780
DpnII GATC 4 cut(s) 148, 661, 747, 778
DraI TTTAAA 2 cut(s) 414, 423
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco105I TACGTA 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 657
Eco147I AGGCCT 1 cut(s) 762
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 132
Eco47III AGCGCT 1 cut(s) 621
EcoNI CCTNNNNNAGG 1 cut(s) 767
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 657
EcoT22I ATGCAT 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 2 cut(s) 644, 661
FaiI YATR 5 cut(s) 137, 228, 284, 642, 659
FaqI GGGAC 1 cut(s) 342
FatI CATG 2 cut(s) 640, 657
FbaI TGATCA 1 cut(s) 747
Fnu4HI GCNGC 3 cut(s) 48, 209, 623
Fsp4HI GCNGC 3 cut(s) 48, 209, 623
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 621
GluI GCNGC 3 cut(s) 48, 209, 623
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 3 cut(s) 47, 78, 762
HapII CCGG 2 cut(s) 52, 66
HhaI GCGC 1 cut(s) 622
Hin1II CATG 2 cut(s) 644, 661
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 2 cut(s) 400, 416
HinfI GANTC 7 cut(s) 29, 86, 377, 458, 527, 684, 734
HpaII CCGG 2 cut(s) 52, 66
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 2 cut(s) 172, 202
Hpy188I TCNGA 1 cut(s) 467
Hpy188III TCNNGA 6 cut(s) 107, 119, 146, 455, 482, 524
Hpy8I GTNNAC 2 cut(s) 172, 202
HpyAV CCTTC 3 cut(s) 601, 647, 674
HpyCH4III ACNGT 2 cut(s) 333, 614
HpyCH4IV ACGT 4 cut(s) 24, 139, 311, 549
HpyCH4V TGCA 5 cut(s) 9, 208, 371, 537, 625
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 44, 75
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 4 cut(s) 24, 139, 311, 549
Hsp92II CATG 2 cut(s) 644, 661
HspAI GCGC 1 cut(s) 620
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 4 cut(s) 148, 661, 747, 778
Lsp1109I GCAGC 2 cut(s) 220, 609
MaeI CTAG 1 cut(s) 266
MaeII ACGT 4 cut(s) 24, 139, 311, 549
MaeIII GTNAC 2 cut(s) 304, 555
MalI GATC 4 cut(s) 150, 663, 749, 780
MboI GATC 4 cut(s) 148, 661, 747, 778
MboII GAAGA 6 cut(s) 109, 443, 497, 527, 721, 724
MfeI CAATTG 1 cut(s) 510
MlyI GAGTC 2 cut(s) 386, 728
MnlI CCTC 5 cut(s) 89, 242, 696, 699, 763
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 2 cut(s) 519, 716
MseI TTAA 4 cut(s) 275, 413, 422, 443
MslI CAYNNNNRTG 1 cut(s) 179
MspI CCGG 2 cut(s) 52, 66
MspR9I CCNGG 2 cut(s) 52, 125
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 373
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 15, 44, 75
NciI CCSGG 1 cut(s) 52
NcoI CCATGG 1 cut(s) 657
NdeII GATC 4 cut(s) 148, 661, 747, 778
NlaIII CATG 2 cut(s) 644, 661
NmuCI GTSAC 1 cut(s) 555
NsiI ATGCAT 1 cut(s) 373
NspV TTCGAA 2 cut(s) 32, 755
PceI AGGCCT 1 cut(s) 762
PctI GAATGC 1 cut(s) 373
PdmI GAANNNNTTC 2 cut(s) 519, 716
PfeI GAWTC 5 cut(s) 29, 86, 458, 527, 684
PkrI GCNGC 3 cut(s) 49, 210, 624
PleI GAGTC 2 cut(s) 385, 728
PpsI GAGTC 2 cut(s) 385, 728
Ppu21I YACGTR 2 cut(s) 140, 550
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspPI GGNCC 2 cut(s) 45, 132
PstI CTGCAG 2 cut(s) 210, 627
PstNI CAGNNNCTG 1 cut(s) 214
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 4 cut(s) 275, 413, 422, 443
SatI GCNGC 3 cut(s) 48, 209, 623
Sau3AI GATC 4 cut(s) 148, 661, 747, 778
Sau96I GGNCC 2 cut(s) 45, 132
SchI GAGTC 2 cut(s) 386, 728
ScrFI CCNGG 2 cut(s) 52, 125
SfcI CTRYAG 2 cut(s) 206, 623
SfuI TTCGAA 2 cut(s) 32, 755
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SnaBI TACGTA 1 cut(s) 140
SpeI ACTAGT 1 cut(s) 265
SseBI AGGCCT 1 cut(s) 762
SsiI CCGC 2 cut(s) 48, 406
SspI AATATT 1 cut(s) 631
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 1 cut(s) 762
StyD4I CCNGG 2 cut(s) 50, 123
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 2 cut(s) 333, 614
TaiI ACGT 4 cut(s) 27, 142, 314, 552
TaqI TCGA 4 cut(s) 32, 301, 720, 755
TaqII GACCGA 1 cut(s) 255
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 5 cut(s) 29, 86, 458, 527, 684
Tru1I TTAA 4 cut(s) 275, 413, 422, 443
Tru9I TTAA 4 cut(s) 275, 413, 422, 443
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 2 cut(s) 208, 622
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 3 cut(s) 126, 336, 629
TspGWI ACGGA 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 132
XagI CCTNNNNNAGG 1 cut(s) 767
XapI RAATTY 2 cut(s) 409, 424
XmnI GAANNNNTTC 2 cut(s) 519, 716
XspI CTAG 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.