pycom14g18300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010332
Physical Location & Seq
Reverse (-)
19987304 .. 19987792
489 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGTTCGTCATCTGCATGCAAGCGCCGATTCCGACTCGGACTCGTCGATGCGCCTGAAGTCCAAAGCCCGTATGCTGGTCTCCATCTCGGCACTCCTTGCCATCGGCCGCCGCCTCTCGGGGAAGCTGAAAGCCGCCAACTCCAAGCTGATAAAAAACAACCACCACTCGGATATCGCGCCGAATAAGTCGCCGATGATGAGAAAGCCCAAGCAGCTGATGTCGACCATAAGCAACAAGGCCATCACCTTCCTGCACCGGAAAAAGATCGGCGAGGAATCGGATGGTAGGGCCCACGCCGTGGAGGAGGAGTGGGGGGACGGTGGGGTATGGCAGAGGTCGATCCTGATGGGGGATAAGTGCGAGCCGTTGGATTTCTCAGGCGCAATTTACTACGATAGTAACGGGAAGAAACTTAACGGAGCTCCCCTCCGGTCCCCACGTGCAAGTCCGATGCCGGGGTACCTCGAAAGGGTCAACCGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.94

Weight (kDa)

10.09

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 462
AccB7I CCANNNNNTGG 1 cut(s) 301
AccI GTMKAC 1 cut(s) 224
AccII CGCG 1 cut(s) 179
AciI CCGC 4 cut(s) 109, 112, 135, 481
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 77
AcvI CACGTG 1 cut(s) 443
AdeI CACNNNGTG 1 cut(s) 301
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 7 cut(s) 76, 118, 169, 301, 352, 458, 472
AluBI AGCT 4 cut(s) 127, 148, 217, 425
AluI AGCT 4 cut(s) 127, 148, 217, 425
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 3 cut(s) 106, 240, 291
ApaI GGGCCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 214
Asp718I GGTACC 1 cut(s) 462
AspLEI GCGC 4 cut(s) 26, 54, 181, 386
AspS9I GGNCC 3 cut(s) 291, 292, 435
AsuC2I CCSGG 1 cut(s) 459
AsuHPI GGTGA 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 295
BanI GGYRCC 1 cut(s) 462
BanII GRGCYC 2 cut(s) 295, 427
BbrPI CACGTG 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 226
BccI CCATC 5 cut(s) 92, 110, 251, 278, 343
BceAI ACGGC 2 cut(s) 284, 352
BcnI CCSGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 27
BisI GCNGC 4 cut(s) 109, 112, 135, 215
BlsI GCNGC 4 cut(s) 110, 113, 136, 216
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 1 cut(s) 435
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 3 cut(s) 291, 292, 435
BmiI GGNNCC 3 cut(s) 293, 437, 464
BmrFI CCNGG 1 cut(s) 459
BmsI GCATC 2 cut(s) 39, 444
BplI GAGNNNNNCTC 2 cut(s) 414, 446
BpuMI CCSGG 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 300, 458
BsaWI WCCGGW 2 cut(s) 258, 432
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 7 cut(s) 76, 118, 169, 301, 352, 458, 472
BseDI CCNNGG 2 cut(s) 300, 458
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 7 cut(s) 76, 118, 169, 301, 352, 458, 472
BseMII CTCAG 1 cut(s) 393
BseRI GAGGAG 2 cut(s) 320, 323
BseSI GKGCMC 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 239
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 108, 242, 293
BshNI GGYRCC 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 3 cut(s) 259, 433, 458
BslFI GGGAC 2 cut(s) 332, 421
BslI CCNNNNNNNGG 7 cut(s) 76, 118, 169, 301, 352, 458, 472
BsmAI GTCTC 1 cut(s) 85
BsmFI GGGAC 2 cut(s) 332, 421
BsnI GGCC 3 cut(s) 108, 242, 293
Bso31I GGTCTC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 118
Bsp120I GGGCCC 1 cut(s) 291
Bsp1286I GDGCHC 2 cut(s) 295, 427
Bsp143I GATC 2 cut(s) 267, 342
BspACI CCGC 4 cut(s) 109, 112, 135, 481
BspANI GGCC 3 cut(s) 108, 242, 293
BspCNI CTCAG 1 cut(s) 392
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 3 cut(s) 293, 437, 464
BspPI GGATC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 462
BspTNI GGTCTC 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 300, 458
BssMI GATC 2 cut(s) 267, 342
Bst4CI ACNGT 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 18, 22, 365
BstDEI CTNAG 1 cut(s) 379
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 179
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 4 cut(s) 26, 54, 181, 386
BstKTI GATC 2 cut(s) 270, 345
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 267, 342
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 2 cut(s) 98, 214
BstNSI RCATGY 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 457
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 3 cut(s) 108, 242, 293
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 18, 22, 365
CfoI GCGC 4 cut(s) 26, 54, 181, 386
Cfr13I GGNCC 3 cut(s) 291, 292, 435
Csp6I GTAC 1 cut(s) 463
CviAII CATG 1 cut(s) 17
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 379
DpnI GATC 2 cut(s) 269, 344
DpnII GATC 2 cut(s) 267, 342
DraIII CACNNNGTG 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 106
EagI CGGCCG 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 425
EclXI CGGCCG 1 cut(s) 106
Eco24I GRGCYC 2 cut(s) 295, 427
Eco31I GGTCTC 1 cut(s) 85
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 435
Eco52I CGGCCG 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 77
Eco72I CACGTG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 291
EcoRV GATATC 1 cut(s) 175
EcoT38I GRGCYC 2 cut(s) 295, 427
FaeI CATG 1 cut(s) 20
FaiI YATR 4 cut(s) 18, 74, 230, 331
FaqI GGGAC 2 cut(s) 332, 421
FatI CATG 1 cut(s) 16
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 4 cut(s) 109, 112, 135, 215
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 2 cut(s) 295, 427
Fsp4HI GCNGC 4 cut(s) 109, 112, 135, 215
GlaI GCGC 4 cut(s) 25, 53, 180, 385
GluI GCNGC 4 cut(s) 109, 112, 135, 215
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 3 cut(s) 108, 242, 293
HapII CCGG 3 cut(s) 259, 433, 458
HhaI GCGC 4 cut(s) 26, 54, 181, 386
Hin1II CATG 1 cut(s) 20
Hin6I GCGC 4 cut(s) 24, 52, 179, 384
HinP1I GCGC 4 cut(s) 24, 52, 179, 384
HincII GTYRAC 2 cut(s) 225, 478
HindII GTYRAC 2 cut(s) 225, 478
HinfI GANTC 4 cut(s) 29, 35, 41, 278
HpaII CCGG 3 cut(s) 259, 433, 458
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 225, 478
Hpy188I TCNGA 5 cut(s) 34, 40, 172, 283, 453
Hpy188III TCNNGA 1 cut(s) 346
Hpy8I GTNNAC 2 cut(s) 225, 478
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 4 cut(s) 16, 20, 256, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 214
HpyF3I CTNAG 1 cut(s) 379
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 1 cut(s) 20
HspAI GCGC 4 cut(s) 24, 52, 179, 384
KpnI GGTACC 1 cut(s) 466
Kzo9I GATC 2 cut(s) 267, 342
LmnI GCTCC 2 cut(s) 422, 430
LpnPI CCDG 8 cut(s) 62, 68, 266, 272, 359, 366, 446, 471
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 2 cut(s) 39, 444
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 1 cut(s) 401
MalI GATC 2 cut(s) 269, 344
MboI GATC 2 cut(s) 267, 342
MboII GAAGA 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 295, 427
MluCI AATT 1 cut(s) 387
MlyI GAGTC 2 cut(s) 29, 35
MmeI TCCRAC 2 cut(s) 57, 351
MnlI CCTC 7 cut(s) 125, 268, 298, 301, 330, 440, 476
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 3 cut(s) 259, 433, 458
MspR9I CCNGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 98, 214
NciI CCSGG 1 cut(s) 459
NdeII GATC 2 cut(s) 267, 342
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 3 cut(s) 293, 437, 464
NmeAIII GCCGAG 1 cut(s) 68
NspI RCATGY 1 cut(s) 20
PaeI GCATGC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 2 cut(s) 29, 278
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 4 cut(s) 110, 113, 136, 216
PleI GAGTC 2 cut(s) 29, 35
PmaCI CACGTG 1 cut(s) 443
PmlI CACGTG 1 cut(s) 443
PpsI GAGTC 2 cut(s) 29, 35
Ppu21I YACGTR 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 427
PspCI CACGTG 1 cut(s) 443
PspN4I GGNNCC 3 cut(s) 293, 437, 464
PspOMI GGGCCC 1 cut(s) 291
PspPI GGNCC 3 cut(s) 291, 292, 435
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 15
SacI GAGCTC 1 cut(s) 427
SalI GTCGAC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 4 cut(s) 109, 112, 135, 215
Sau3AI GATC 2 cut(s) 267, 342
Sau96I GGNCC 3 cut(s) 291, 292, 435
SchI GAGTC 2 cut(s) 29, 35
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 2 cut(s) 295, 427
SetI ASST 8 cut(s) 129, 150, 219, 251, 341, 427, 445, 468
SfaNI GCATC 2 cut(s) 39, 444
SinI GGWCC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 15
SphI GCATGC 1 cut(s) 20
Sse9I AATT 1 cut(s) 387
SsiI CCGC 4 cut(s) 109, 112, 135, 481
SstI GAGCTC 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 457
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 445
TaqI TCGA 4 cut(s) 47, 224, 341, 468
TasI AATT 1 cut(s) 387
TauI GCSGC 3 cut(s) 111, 114, 137
TfiI GAWTC 2 cut(s) 29, 278
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseI GCWGC 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 435
Van91I CCANNNNNTGG 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 435
XceI RCATGY 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.