Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0004278

Physical Location & Seq

Genomic Coordinates Forward (+)

20370139 .. 20373915

Gene Structure 3777 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGGATTCCGCATCCAATTACAGCAGAAAAGAAAATGGAACCAGCAGCTCAAGCGTTCCTGCTACCGGGAATAGTTTCGAACAAGTTGTAAATGGTAATGATGAGACTGAAGGCAAGCGGCGAGCATACTTAGTAGAATGGTTAAATAGTTTAGTTCCTAATCTAGGTTTACCAACAACTGCTTCTGATGAGGACTTGAGATCGTGCTTGATCGATGGTACTGTTTTATGTTGGATATTGAACAGGCTTAGACCTGGTCTTATTGATGAGAACTCGGTTCCACCCTCAGAAAAAGTTGCAAGGTTTCTGGCAGCTATGGATGCACTGGGGCTGCCGAAGTTTGATATGTCCGACCTAGAGAAGGTACCTATCAATGTAATTTGTGTGCATACAGATCCCTTGCAATGTGGTTCTATGAAGGCTGTCACAGAATGTCTTTTAACACTTAAAGCACGATTTATGCCAAATGTCATGGGTTATGGTGTATCTATAACTAGTCCGACGACTAAATCTGGCAGCCAATCTTCCCGTGTCCAGCTTGCTCCATTATCTGTAGCTGACACGCGGAAAGTCTTGTCTGAGTCAAAATTTCAGCGCGCATTGCGTAGTCCTGTTATGTCAGGAGAGACGTCACCTTTACTCATGCATCATGTCGGACATAAGTTCCACGAGGTGTTTCAGTTGAAGCAAGGAGGCTACGCAGATCTTCCTGCTGCAAAAATTTCAGAAATGATGAAACCAAACAGTTTAGATAACGCTCCAACGCAGTCACTTTTGAGTGTCGTAAATGGAATCCTCGATGAAAGTGTTGAAAGAAAGAATGGTGAAATACCCCATGTATGGAAACCCGAAATGGGAATGTTTTACAATTGGCAGAATGAGAGAACCCGAATGGAAGCAAAGAAGAAAACCGATGATGAGGATGTGATCAGATTAATGAAAGAGAAGGATCAATGCAACCTTGAAATTTCAGGATTGAAGCAAGAGTTGGATATAGCGAAAAAGACATATGACCTGCGTTGCTTGCAAATGGAAACGGAAGCAAAAGGTGCTAGGGCGGAGCTTGAAGAGAGGACAAAAGAACTCGAACGTCTCTTGGCAGAGTCGAGGAGTAAGGTGAAGGAGCTCCAGGAAAATTCTGAGTCAAAACATCAGTCTAATAAAGCAGAACTTGAAGAGACAGTAAAAGAACTTGAACGTTCTTTGGCAGAATCAAGGAATAAAGTGAAAGAACTCGAGGCAAGTTCCGAGTCCAAATATCAGTTTTCTAAAGCAGAGCTTGAAGGGAGAATAAAAGAACTTGAACGCCTCTTGGCAGATTCAAGGAATGAGGCAAAGCAGCTTTCAGCGAATTCAGATTCAAAATATAAAAGTTGGACCAAGAAACTGCGTGTTTACGAGACCTCTATGGATCTTCAACTTGGTTCACTAAAGGAATTAAGGTTATCATCCGAATCCATTAAGCAAGAAGTTTTGACGGCAGGACAGAGTTACACGGTGAACTTTAATCACTTAGGTAGGCTTCAAGCATTAGCAGACGCGTCTGAGAACTATCATGCACTTCTCGCTGAAAATAGGAAGTTATTTAATGAAATTCAGGATTTAAGAGGAAACATCAGAGTCTTCTGTCGAATAAGACCATTTCTTCCTGGACAAAAAGAAAAGAAGACATCAGTAGAACATACTGGTGAAAATGGGGAACTGGTAGTTGCAGACCGCTCCAAACCAGGAAAAGAGGGTCATCGATTGTTCAAGTTTAATAAGGTCTATGGTTCAGATGCAACTCAAGGTTCTCTATTTTCTGAAGTATACGCTGACACACAACCGTTGATACGATCTGTGCTTGATGGATATAATGTTTGTATTTGTGCTTATGGTCAAACTGGATCCGGGAAAACCTACACGATGACTGGTCCTAATCAAGCAAGTAAAGAGAACTGGGGGGTCAACTACCGGGCCTTAAACGACCTTTTTGACATCTCTCAATGTAGAAAAAGCTCCATTACATATGAAATTGGAGTCCAAATGGTTGAAATATACAACGAACAAGTGCGCGATTTGCTATCCAGTGATGGTGCTCAGAAGAAATATCCTTGTCGTTACGAAATGCGCTTCCTATTATTTTGCATACTTATATGTCGTCTGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

717

Amino Acids

80.6

Weight (kDa)

6.86

Isoelectric Point (pI)

42.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	41 - 120	2.2e-08	Calponin homology (CH) domain
Microtub_bd	PF16796	524 - 688	2.6e-38	Microtubule binding
Kinesin	PF00225	544 - 697	3.5e-37	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G73860 AT1G73860
fragaria_vesca	FvH4_5g12130 FvH4_5g12130
malus_domestica	MD06G1216000.v1.1 MD14G1226600.v1.1
prunus_persica	Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1
pyrus_communis	pycom06g19260 pycom14g18800
rosa_chinensis	RchiOBHm_Chr7g0185441 RchiOBHm_Chr7g0185451
rosa_laevigata	RLG00000004912
rosa_multiflora	Rmu_sc0010190.1_g000028
rosa_roxburghii	Rroxscaffold_3G00268930
rosa_rugosa	Rorug06G0469100 Rorug06G0469200 Rorug06G0469300 Rorug06G0469400 Rorug06G0469500
rosa_samantha	Rh7AG073500 Rh7BG074100 Rh7CG074300 Rh7DG074200
rosa_wichuraiana	Rw7G006170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	632
Acc36I	ACCTGC	1 cut(s)	1023
Acc65I	GGTACC	1 cut(s)	364
AccB1I	GGYRCC	1 cut(s)	364
AccBSI	CCGCTC	1 cut(s)	1719
AccI	GTMKAC	1 cut(s)	1809
AccII	CGCG	4 cut(s)	565, 597, 1541, 2055
AciI	CCGC	5 cut(s)	9, 118, 565, 1058, 1717
AclI	AACGTT	1 cut(s)	1198
AclWI	GGATC	5 cut(s)	389, 957, 1419, 1881, 1894
AcsI	RAATTY	6 cut(s)	587, 720, 966, 1135, 1351, 1593
AcuI	CTGAAG	2 cut(s)	129, 1824
AcyI	GRCGYC	1 cut(s)	629
AdeI	CACNNNGTG	1 cut(s)	673
AfaI	GTAC	2 cut(s)	220, 366
AfiI	CCNNNNNNNGG	5 cut(s)	65, 164, 361, 840, 854
AflIII	ACRYGT	1 cut(s)	1539
AhlI	ACTAGT	1 cut(s)	494
AjnI	CCWGG	4 cut(s)	253, 1128, 1648, 1726
AjuI	GAANNNNNNNTTGG	4 cut(s)	166, 198, 971, 1003
AluBI	AGCT	9 cut(s)	48, 314, 538, 557, 1063, 1126, 1279, 1342, 1998
AluI	AGCT	9 cut(s)	48, 314, 538, 557, 1063, 1126, 1279, 1342, 1998
Alw21I	GWGCWC	2 cut(s)	1128, 2080
Alw26I	GTCTC	5 cut(s)	98, 620, 1097, 1172, 1394
AlwI	GGATC	5 cut(s)	389, 957, 1419, 1881, 1894
Ama87I	CYCGRG	1 cut(s)	1235
AoxI	GGCC	1 cut(s)	1956
ApeKI	GCWGC	6 cut(s)	45, 311, 331, 516, 713, 1339
ApoI	RAATTY	6 cut(s)	587, 720, 966, 1135, 1351, 1593
ArsI	GACNNNNNNTTYG	2 cut(s)	1139, 1171
AseI	ATTAAT	1 cut(s)	935
Asp700I	GAANNNNTTC	1 cut(s)	74
Asp718I	GGTACC	1 cut(s)	364
AspLEI	GCGC	4 cut(s)	597, 599, 2055, 2112
AspS9I	GGNCC	3 cut(s)	1377, 1913, 1956
AsuC2I	CCSGG	3 cut(s)	67, 1891, 1955
AsuHPI	GGTGA	5 cut(s)	624, 836, 1129, 1510, 1700
AsuII	TTCGAA	1 cut(s)	78
AvaI	CYCGRG	1 cut(s)	1235
AvaII	GGWCC	2 cut(s)	1377, 1913
BamHI	GGATCC	1 cut(s)	1886
BanI	GGYRCC	1 cut(s)	364
BanII	GRGCYC	1 cut(s)	1128
BauI	CACGAG	1 cut(s)	668
BbsI	GAAGAC	2 cut(s)	1615, 1673
Bbv12I	GWGCWC	2 cut(s)	1128, 2080
BbvI	GCAGC	6 cut(s)	57, 318, 323, 528, 700, 1351
BccI	CCATC	3 cut(s)	209, 1841, 2066
BceAI	ACGGC	1 cut(s)	1494
BciT130I	CCWGG	4 cut(s)	255, 1130, 1650, 1728
BclI	TGATCA	1 cut(s)	927
BcnI	CCSGG	3 cut(s)	67, 1891, 1955
BcoDI	GTCTC	5 cut(s)	98, 620, 1097, 1172, 1394
BcuI	ACTAGT	1 cut(s)	494
BfaI	CTAG	4 cut(s)	164, 356, 495, 1053
BfmI	CTRYAG	1 cut(s)	552
BfuAI	ACCTGC	1 cut(s)	1023
BglII	AGATCT	1 cut(s)	703
BisI	GCNGC	7 cut(s)	46, 119, 312, 332, 517, 714, 1340
BlsI	GCNGC	7 cut(s)	47, 120, 313, 333, 518, 715, 1341
Bme1390I	CCNGG	7 cut(s)	67, 255, 1130, 1650, 1728, 1891, 1955
Bme18I	GGWCC	2 cut(s)	1377, 1913
BmeT110I	CYCGRG	1 cut(s)	1235
BmgT120I	GGNCC	3 cut(s)	1377, 1913, 1956
BmiI	GGNNCC	4 cut(s)	40, 279, 366, 1888
BmrFI	CCNGG	7 cut(s)	67, 255, 1130, 1650, 1728, 1891, 1955
BmrI	ACTGGG	2 cut(s)	335, 1948
BmsI	GCATC	4 cut(s)	20, 310, 655, 1768
BmuI	ACTGGG	2 cut(s)	335, 1948
BpiI	GAAGAC	2 cut(s)	1615, 1673
BpmI	CTGGAG	1 cut(s)	1112
Bpu14I	TTCGAA	1 cut(s)	78
BpuEI	CTTGAG	3 cut(s)	34, 217, 1770
BpuMI	CCSGG	3 cut(s)	67, 1891, 1955
Bsa29I	ATCGAT	2 cut(s)	213, 1744
BsaHI	GRCGYC	1 cut(s)	629
BsaI	GGTCTC	1 cut(s)	1394
Bsc4I	CCNNNNNNNGG	5 cut(s)	65, 164, 361, 840, 854
Bse1I	ACTGG	7 cut(s)	330, 1690, 1707, 1888, 1915, 1943, 2067
Bse3DI	GCAATG	2 cut(s)	410, 599
BseBI	CCWGG	4 cut(s)	255, 1130, 1650, 1728
BseCI	ATCGAT	2 cut(s)	213, 1744
BseGI	GGATG	4 cut(s)	11, 325, 928, 1448
BseLI	CCNNNNNNNGG	5 cut(s)	65, 164, 361, 840, 854
BseMI	GCAATG	2 cut(s)	410, 599
BseMII	CTCAG	6 cut(s)	300, 570, 1131, 1536, 2093, 2135
BseNI	ACTGG	7 cut(s)	330, 1690, 1707, 1888, 1915, 1943, 2067
BsePI	GCGCGC	1 cut(s)	595
BseRI	GAGGAG	1 cut(s)	1123
BseXI	GCAGC	6 cut(s)	57, 318, 323, 528, 700, 1351
Bsh1236I	CGCG	4 cut(s)	565, 597, 1541, 2055
BshFI	GGCC	1 cut(s)	1958
BshNI	GGYRCC	1 cut(s)	364
BshVI	ATCGAT	2 cut(s)	213, 1744
BsiHKAI	GWGCWC	2 cut(s)	1128, 2080
BsiHKCI	CYCGRG	1 cut(s)	1235
BsiSI	CCGG	3 cut(s)	66, 1890, 1954
BslI	CCNNNNNNNGG	5 cut(s)	65, 164, 361, 840, 854
BsmAI	GTCTC	5 cut(s)	98, 620, 1097, 1172, 1394
BsmBI	CGTCTC	2 cut(s)	620, 1097
BsnI	GGCC	1 cut(s)	1958
Bso31I	GGTCTC	1 cut(s)	1394
BsoBI	CYCGRG	1 cut(s)	1235
Bsp119I	TTCGAA	1 cut(s)	78
Bsp1286I	GDGCHC	2 cut(s)	1128, 2080
Bsp143I	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
BspACI	CCGC	5 cut(s)	9, 118, 565, 1058, 1717
BspANI	GGCC	1 cut(s)	1958
BspCNI	CTCAG	6 cut(s)	299, 571, 1132, 1537, 2092, 2136
BspDI	ATCGAT	2 cut(s)	213, 1744
BspFNI	CGCG	4 cut(s)	565, 597, 1541, 2055
BspLI	GGNNCC	4 cut(s)	40, 279, 366, 1888
BspMI	ACCTGC	1 cut(s)	1023
BspPI	GGATC	5 cut(s)	389, 957, 1419, 1881, 1894
BspT104I	TTCGAA	1 cut(s)	78
BspT107I	GGYRCC	1 cut(s)	364
BspTNI	GGTCTC	1 cut(s)	1394
BsrBI	CCGCTC	1 cut(s)	1719
BsrDI	GCAATG	2 cut(s)	410, 599
BsrI	ACTGG	7 cut(s)	330, 1690, 1707, 1888, 1915, 1943, 2067
BssHII	GCGCGC	1 cut(s)	595
BssMI	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
BssNAI	GTATAC	1 cut(s)	1810
BssNI	GRCGYC	1 cut(s)	629
BssSI	CACGAG	1 cut(s)	668
Bst1107I	GTATAC	1 cut(s)	1810
Bst2BI	CACGAG	1 cut(s)	668
Bst2UI	CCWGG	4 cut(s)	255, 1130, 1650, 1728
Bst4CI	ACNGT	5 cut(s)	223, 746, 1183, 1498, 1827
Bst6I	CTCTTC	2 cut(s)	1062, 1170
BstACI	GRCGYC	1 cut(s)	629
BstAPI	GCANNNNNTGC	1 cut(s)	1049
BstBI	TTCGAA	1 cut(s)	78
BstC8I	GCNNGC	5 cut(s)	116, 123, 540, 597, 1025
BstDEI	CTNAG	9 cut(s)	130, 248, 286, 579, 1140, 1513, 1545, 2079, 2144
BstENI	CCTNNNNNAGG	2 cut(s)	162, 359
BstF5I	GGATG	4 cut(s)	11, 325, 928, 1448
BstFNI	CGCG	4 cut(s)	565, 597, 1541, 2055
BstHHI	GCGC	4 cut(s)	597, 599, 2055, 2112
BstKTI	GATC	9 cut(s)	203, 213, 397, 706, 930, 952, 1414, 1838, 1889
BstMAI	GTCTC	5 cut(s)	98, 620, 1097, 1172, 1394
BstMBI	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
BstMWI	GCNNNNNNNGC	7 cut(s)	51, 320, 601, 1024, 1049, 1565, 2059
BstNI	CCWGG	4 cut(s)	255, 1130, 1650, 1728
BstSCI	CCNGG	7 cut(s)	65, 253, 1128, 1648, 1726, 1889, 1953
BstSFI	CTRYAG	1 cut(s)	552
BstUI	CGCG	4 cut(s)	565, 597, 1541, 2055
BstV1I	GCAGC	6 cut(s)	57, 318, 323, 528, 700, 1351
BstV2I	GAAGAC	2 cut(s)	1615, 1673
BstX2I	RGATCY	4 cut(s)	394, 703, 1411, 1886
BstYI	RGATCY	4 cut(s)	394, 703, 1411, 1886
BstZ17I	GTATAC	1 cut(s)	1810
Bsu15I	ATCGAT	2 cut(s)	213, 1744
BsuRI	GGCC	1 cut(s)	1958
BsuTUI	ATCGAT	2 cut(s)	213, 1744
BtsCI	GGATG	4 cut(s)	11, 325, 928, 1448
BtsIMutI	CAGTG	2 cut(s)	323, 2074
BveI	ACCTGC	1 cut(s)	1023
Cac8I	GCNNGC	5 cut(s)	116, 123, 540, 597, 1025
CfoI	GCGC	4 cut(s)	597, 599, 2055, 2112
Cfr13I	GGNCC	3 cut(s)	1377, 1913, 1956
ClaI	ATCGAT	2 cut(s)	213, 1744
CseI	GACGC	2 cut(s)	1530, 1547
CsiI	ACCWGGT	1 cut(s)	253
Csp6I	GTAC	2 cut(s)	219, 365
CviAII	CATG	5 cut(s)	472, 643, 650, 836, 1556
CviQI	GTAC	2 cut(s)	219, 365
DdeI	CTNAG	9 cut(s)	130, 248, 286, 579, 1140, 1513, 1545, 2079, 2144
DpnI	GATC	9 cut(s)	202, 212, 396, 705, 929, 951, 1413, 1837, 1888
DpnII	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
DraIII	CACNNNGTG	1 cut(s)	673
Eam1104I	CTCTTC	2 cut(s)	1062, 1170
EarI	CTCTTC	2 cut(s)	1062, 1170
EciI	GGCGGA	1 cut(s)	1073
Ecl136II	GAGCTC	1 cut(s)	1126
Eco24I	GRGCYC	1 cut(s)	1128
Eco31I	GGTCTC	1 cut(s)	1394
Eco47I	GGWCC	2 cut(s)	1377, 1913
Eco53kI	GAGCTC	1 cut(s)	1126
Eco57I	CTGAAG	2 cut(s)	129, 1824
Eco88I	CYCGRG	1 cut(s)	1235
EcoICRI	GAGCTC	1 cut(s)	1126
EcoNI	CCTNNNNNAGG	2 cut(s)	162, 359
EcoRI	GAATTC	1 cut(s)	1351
EcoRII	CCWGG	4 cut(s)	253, 1128, 1648, 1726
EcoT22I	ATGCAT	1 cut(s)	648
EcoT38I	GRGCYC	1 cut(s)	1128
Esp3I	CGTCTC	2 cut(s)	620, 1097
FaeI	CATG	5 cut(s)	475, 646, 653, 839, 1559
FalI	AAGNNNNNCTT	4 cut(s)	1155, 1187, 1263, 1295
FatI	CATG	5 cut(s)	471, 642, 649, 835, 1555
FauNDI	CATATG	2 cut(s)	1009, 2008
FbaI	TGATCA	1 cut(s)	927
FblI	GTMKAC	1 cut(s)	1809
Fnu4HI	GCNGC	7 cut(s)	46, 119, 312, 332, 517, 714, 1340
FokI	GGATG	3 cut(s)	332, 935, 1435
FriOI	GRGCYC	1 cut(s)	1128
Fsp4HI	GCNGC	7 cut(s)	46, 119, 312, 332, 517, 714, 1340
FspBI	CTAG	4 cut(s)	164, 356, 495, 1053
GlaI	GCGC	4 cut(s)	596, 598, 2054, 2111
GluI	GCNGC	7 cut(s)	46, 119, 312, 332, 517, 714, 1340
GsuI	CTGGAG	1 cut(s)	1112
HaeIII	GGCC	1 cut(s)	1958
HapII	CCGG	3 cut(s)	66, 1890, 1954
HgaI	GACGC	2 cut(s)	1530, 1547
HhaI	GCGC	4 cut(s)	597, 599, 2055, 2112
Hin1I	GRCGYC	1 cut(s)	629
Hin1II	CATG	5 cut(s)	475, 646, 653, 839, 1559
Hin6I	GCGC	4 cut(s)	595, 597, 2053, 2110
HinP1I	GCGC	4 cut(s)	595, 597, 2053, 2110
HincII	GTYRAC	1 cut(s)	1948
HindII	GTYRAC	1 cut(s)	1948
HpaII	CCGG	3 cut(s)	66, 1890, 1954
HphI	GGTGA	5 cut(s)	624, 836, 1129, 1510, 1700
Hpy166II	GTNNAC	6 cut(s)	170, 1396, 1427, 1501, 1810, 1948
Hpy188III	TCNNGA	3 cut(s)	621, 972, 1598
Hpy8I	GTNNAC	6 cut(s)	170, 1396, 1427, 1501, 1810, 1948
Hpy99I	CGWCG	1 cut(s)	505
HpyAV	CCTTC	6 cut(s)	104, 355, 412, 940, 1114, 1277
HpyCH4III	ACNGT	5 cut(s)	223, 746, 1183, 1498, 1827
HpyCH4IV	ACGT	3 cut(s)	629, 1090, 1198
HpyF10VI	GCNNNNNNNGC	7 cut(s)	51, 320, 601, 1024, 1049, 1565, 2059
HpyF3I	CTNAG	9 cut(s)	130, 248, 286, 579, 1140, 1513, 1545, 2079, 2144
HpySE526I	ACGT	3 cut(s)	629, 1090, 1198
Hsp92I	GRCGYC	1 cut(s)	629
Hsp92II	CATG	5 cut(s)	475, 646, 653, 839, 1559
HspAI	GCGC	4 cut(s)	595, 597, 2053, 2110
KpnI	GGTACC	1 cut(s)	368
Ksp22I	TGATCA	1 cut(s)	927
Kzo9I	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
LmnI	GCTCC	7 cut(s)	547, 763, 1060, 1123, 1131, 1724, 2003
Lsp1109I	GCAGC	6 cut(s)	57, 318, 323, 528, 700, 1351
LweI	GCATC	4 cut(s)	20, 310, 655, 1768
MabI	ACCWGGT	1 cut(s)	253
MaeI	CTAG	4 cut(s)	164, 356, 495, 1053
MaeII	ACGT	3 cut(s)	629, 1090, 1198
MaeIII	GTNAC	5 cut(s)	424, 630, 768, 1490, 2099
MalI	GATC	9 cut(s)	202, 212, 396, 705, 929, 951, 1413, 1837, 1888
MbiI	CCGCTC	1 cut(s)	1719
MboI	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
MfeI	CAATTG	1 cut(s)	868
MflI	RGATCY	4 cut(s)	394, 703, 1411, 1886
MhlI	GDGCHC	2 cut(s)	1128, 2080
MluI	ACGCGT	1 cut(s)	1539
MlyI	GAGTC	6 cut(s)	590, 1112, 1151, 1259, 1629, 2028
MmeI	TCCRAC	7 cut(s)	212, 375, 524, 634, 785, 969, 1355
Mph1103I	ATGCAT	1 cut(s)	648
MroXI	GAANNNNTTC	1 cut(s)	74
MslI	CAYNNNNRTG	1 cut(s)	1686
MspI	CCGG	3 cut(s)	66, 1890, 1954
MspR9I	CCNGG	7 cut(s)	67, 255, 1130, 1650, 1728, 1891, 1955
MunI	CAATTG	1 cut(s)	868
MvaI	CCWGG	4 cut(s)	255, 1130, 1650, 1728
MvnI	CGCG	4 cut(s)	565, 597, 1541, 2055
MwoI	GCNNNNNNNGC	7 cut(s)	51, 320, 601, 1024, 1049, 1565, 2059
NciI	CCSGG	3 cut(s)	67, 1891, 1955
NdeI	CATATG	2 cut(s)	1009, 2008
NdeII	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
NlaIII	CATG	5 cut(s)	475, 646, 653, 839, 1559
NlaIV	GGNNCC	4 cut(s)	40, 279, 366, 1888
NmuCI	GTSAC	3 cut(s)	424, 630, 768
NsiI	ATGCAT	1 cut(s)	648
NspV	TTCGAA	1 cut(s)	78
PaeR7I	CTCGAG	1 cut(s)	1235
PauI	GCGCGC	1 cut(s)	595
PdmI	GAANNNNTTC	1 cut(s)	74
PfeI	GAWTC	6 cut(s)	5, 792, 1211, 1319, 1358, 1454
PflFI	GACNNNGTC	1 cut(s)	255
PfoI	TCCNGGA	3 cut(s)	1128, 1648, 1889
PkrI	GCNGC	7 cut(s)	47, 120, 313, 333, 518, 715, 1341
PleI	GAGTC	6 cut(s)	589, 1111, 1150, 1258, 1628, 2027
PpsI	GAGTC	6 cut(s)	589, 1111, 1150, 1258, 1628, 2027
PshBI	ATTAAT	1 cut(s)	935
Psp124BI	GAGCTC	1 cut(s)	1128
Psp1406I	AACGTT	1 cut(s)	1198
Psp6I	CCWGG	4 cut(s)	253, 1128, 1648, 1726
PspGI	CCWGG	4 cut(s)	253, 1128, 1648, 1726
PspN4I	GGNNCC	4 cut(s)	40, 279, 366, 1888
PspPI	GGNCC	3 cut(s)	1377, 1913, 1956
PspXI	VCTCGAGB	1 cut(s)	1235
PsuI	RGATCY	4 cut(s)	394, 703, 1411, 1886
PsyI	GACNNNGTC	1 cut(s)	255
PteI	GCGCGC	1 cut(s)	595
RsaI	GTAC	2 cut(s)	220, 366
RsaNI	GTAC	2 cut(s)	219, 365
RseI	CAYNNNNRTG	1 cut(s)	1686
SacI	GAGCTC	1 cut(s)	1128
SatI	GCNGC	7 cut(s)	46, 119, 312, 332, 517, 714, 1340
Sau3AI	GATC	9 cut(s)	200, 210, 394, 703, 927, 949, 1411, 1835, 1886
Sau96I	GGNCC	3 cut(s)	1377, 1913, 1956
SchI	GAGTC	6 cut(s)	590, 1112, 1151, 1259, 1629, 2028
ScrFI	CCNGG	7 cut(s)	67, 255, 1130, 1650, 1728, 1891, 1955
SduI	GDGCHC	2 cut(s)	1128, 2080
SexAI	ACCWGGT	1 cut(s)	253
SfaNI	GCATC	4 cut(s)	20, 310, 655, 1768
SfcI	CTRYAG	1 cut(s)	552
Sfr274I	CTCGAG	1 cut(s)	1235
SfuI	TTCGAA	1 cut(s)	78
SinI	GGWCC	2 cut(s)	1377, 1913
SlaI	CTCGAG	1 cut(s)	1235
SmiMI	CAYNNNNRTG	1 cut(s)	1686
SmlI	CTYRAG	4 cut(s)	49, 196, 1235, 1785
SmoI	CTYRAG	4 cut(s)	49, 196, 1235, 1785
SpeI	ACTAGT	1 cut(s)	494
SsiI	CCGC	5 cut(s)	9, 118, 565, 1058, 1717
SspMI	CTAG	4 cut(s)	164, 356, 495, 1053
SstI	GAGCTC	1 cut(s)	1128
StyD4I	CCNGG	7 cut(s)	65, 253, 1128, 1648, 1726, 1889, 1953
TaaI	ACNGT	5 cut(s)	223, 746, 1183, 1498, 1827
TaiI	ACGT	3 cut(s)	632, 1093, 1201
TaqI	TCGA	8 cut(s)	78, 213, 798, 1086, 1106, 1236, 1630, 1744
TauI	GCSGC	1 cut(s)	121
TfiI	GAWTC	6 cut(s)	5, 792, 1211, 1319, 1358, 1454
TscAI	CASTG	2 cut(s)	330, 2074
TseFI	GTSAC	3 cut(s)	424, 630, 768
TseI	GCWGC	6 cut(s)	45, 311, 331, 516, 713, 1339
Tsp45I	GTSAC	3 cut(s)	424, 630, 768
TspDTI	ATGAA	6 cut(s)	431, 749, 816, 953, 1605, 2025
TspGWI	ACGGA	1 cut(s)	1052
TspRI	CASTG	2 cut(s)	330, 2074
Tth111I	GACNNNGTC	1 cut(s)	255
VpaK11BI	GGWCC	2 cut(s)	1377, 1913
VspI	ATTAAT	1 cut(s)	935
XagI	CCTNNNNNAGG	2 cut(s)	162, 359
XapI	RAATTY	6 cut(s)	587, 720, 966, 1135, 1351, 1593
XhoI	CTCGAG	1 cut(s)	1235
XmiI	GTMKAC	1 cut(s)	1809
XmnI	GAANNNNTTC	1 cut(s)	74
XspI	CTAG	4 cut(s)	164, 356, 495, 1053
ZraI	GACGTC	1 cut(s)	630
Zsp2I	ATGCAT	1 cut(s)	648

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.