pycom14g19800

Putative sugar-binding N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006366
Physical Location & Seq
Reverse (-)
21023141 .. 21034877
11737 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4281 bp
ATGGAGAAAAAAAACAAAGATTGGCGCACAACGACGGAAACGTGGAGACGTTTGGCATCCGTAGGACTCTACTCAAGTCCACAATCAAAACTGGTCAGTCCGGTTTTTAGTGACAGTGAGGGTCGTTGCAAAAACCAAGCTATCAGAGCAGCAGAAGAAAGCAGAGCAGCAATGGCGTCCAAGGGAGCTGTTCGGTTCGTCGGCTTGGATGCCTTGAGCTTGGACTTGGCTTCCTCTCTCCTCCGCAGTGGCTACAAAGTCCAAGCTTTTGAGATGGATGGGAATTTGATTAGTGAGTTTTTAAAACTTGGAGGAACCAGATGTGGCGGCCCAGAGGAGGCAGGAAAAGATGTTGCAGCTTTGGTTGTGTTAGCAGATCAAGTAAATGATGCAACTTTCAGTGATGAGGGTGCATCGAAAGGATTTCAGAAGGATACTGTTGTCATACTTCGTTCAACAATATTACCATCGTCTATTCAGAATCTAAAGACGCGTTTCACAGATACAGCTGATCTTGTAGATATATATGCCACAAAGGGAGTATCTGATGGTTTTAATGGAAAGATCATGATCGCTTCTTCCGGAGGGTCAGACGCTATTTTAAAAGCACGGCCAGTTCTCTCCGCAATGTGCGATAAGCTTTATGTTTTTGATGGTGAAGTTGGCGCTGGAAGCAAAATTAGGATGATTAAGGAACTGCTTGAGGGTATTCATCTTGTTGCTTCTTTGGAGGCTATTTCTCTTGGCACCAAAGCCGGTATTCATCCGTGGATAATCTACGATATCATATCTAATGCTGCGGGAAACTCATGGGTTTTCAAGAACCATATTCCTCAGTTGTTAAGGGGTGCTGCCAAAGATGATTTGAATACTCTTGTTCAAAACTTGAGGGTTATATTGGATCTGGCCAAGTCGCTTAGCTTTCCTCTCCCGCTTTTAGCAGTTGCTCATCAGCAACTTATTCTTGGGTCTCCACGTGACAACACAGATGATGAAGATGCTACATTGATTAAGGTTTGGGAACAGAAACTTGGAGTGAGAATTTTAGATGCAGCAAATGCTGAGACTTACATCCCTGAGGAATTGGCAAGCCACACTGTTGCTAAATCATATACCGTCAACCGAGTTGGATTCATTGGTCTTGGAGCAATGGGATTTGGAATGGCAACTCACCTTTTAAAAGCTAAATTTTCTGTTTGTGGTTATGATGTATATAAACCAACACTGACTCGTTTTGCCAATGCTGGTGGCTCGATTGGAGGCTCTCCAGCAGAAGTATGTAAAGATGTTGATGTGCTGGTAATAATGGTCACAAATGAAGCTCAAGCTGAGAGCGCTTTGTATGGAGACTTTGGTGCTATTTCAGCACTTCCATCTGGAGCTTCTATTATCCTTTCATCCACTGTTTCTCCTGGATTTGTGAGCCGACTAGATCACCGTTTGCAAAATGAAGGAAAGAATTTCAAGTTGGTGGATGCTCCTGTGTCTGGTGGGGTTGTGAGAGCCTCCATGGGGGCACTTACGATTATGGCATCCGGTACAGATGAAGCTCTTAAGAGTGCTGGTGCAGTCCTCTCAGCATTAAGCGAGAAGCTTTATGTTATCAAAGGAGGCTGTGGGGCTGGAAGTGGTGTAAAGATGGTTAATCAATTGCTTGCTGGAGTTCATATAGCGTCGGGAGCTGAAGCAATGGCTTTTGGAGCTCGATTGGGTCTTAATACAAGGACGTTGTTTGATTTCATCACAAATAGTGAGGGAACATCCTGGATGTTAGAAAATCGTGTTCCACACATGCTCGACAATGATTATACTCCGCATTCTGCACTTGATATTTTTGTGAAGGATCTGGGAATTGTTTCTCAAGATTGTTCAGTTCGAAAAATTCCACTTCATATATCAACTGTTGCTCATCAACTCTTCCTGTCAGGTTCTGCTGCAGGTTGGGGTCGGCAAGATGATGCTGGTGTGGTTAAGGTTTATGAGACACTAACGGGTGTTAAGGTAGAAGCAAAACTTCCGGTTCTTAAGAAGGACGTTGTCTTCAAGTCTCTCCCAGTTGAGTGGCAAGTAGATCCCACTGGTGAAATACAGAGACTAAATCAGGACAGTTCAAAGACTTTGGTTGTTCTCGATGACGATCCAACTGGAACTCAAACTGTTCATGATATTGAGGTCTTGACGGAATGGACCATTGAATCACTAATGGAGCAGTTCAGAAAAAGTCCTAAATGCTTTTTCATTTTGACCAACTCCAGGGCCTTGAGTTCTGAGAAGGCTACTGCATTAATTAAAGACATCTGCGTAAACCTGCGTACTGCAGCCAAATCAGTTGAGAATGCTGATTATACAGTAGTTTTGAGAGGCGATTCAACTTTGCGTGGCCATTTCCCAGAGGAAGCTGATGCCGCTGTTTCAGTACTGGGCGAGATGGATGCATGGATCATCTGCCCCTTTTTTCTTCAAGGAGGGCGTTATACTGTTGGAGATGTACACTATGTTGCAGATTCGGACCAGCTTATCCCTGCAGGGGATACGGGATTCGCTAAAGATGCTGCTTTTGGCTACAAATCTTCAAACCTTCGCGAGTGGGTTGAGGAGAAAACAGCCGGCCGCATACCAGCCAGTAGTGTTACATCTATTTCTATCCAAATTCTAAGAAAAGGTGGGCCAGATGCTGTTTGTGAGCGTCTTTGCAGTTTGCAGAAGGGATCAACATGCATAGTTAACGCAGCTAGTGACAGAGACATGGCTGTTTTTGCAGCTGGAATGATCAAGGCGGAACTGAAGGGCAAGCGCTTCTTATGTCGCACTGCTGCTAGCTTTGTTTCTGCCAGGATCGGAATTATCCCCAGAGCTCCAATTCTGCCCAAGGATCTAGGAATAAACAAGGAAAGGAACGGAGGTCTCATAGTTGTCGGGTCATATGTTCCCAAAACGACAAAACAGGTGGAAGAGCTTAAACTACAGTGTAGCCAGATTTTGAGAAGCATTGAGGTTTCGGTTGCTAAAGTTGCCATGAGTTCTACAGAAGAGAGGGAGGAGGAAATCAGTAGAGCAGCTGAGATTGCAGATATATTCCTTACAGCTCGGAAGGACACTCTAATAATGACCAGTCGGGAGCTTATTACCGGAAAAACTCCCTCTGAGAGTTTGGAAATCAACTTCAAAGTTAGCTCGGCACTGGTGGAAATAGTGAGACGCATAACTACAAGACCACGTTACATTCTTGCAAAGGGTGGAATCACTTCATCTGACCTTGCTACGAAAGCTCTTGAAGCAAAATGTGCAAAAATAGTGGGACAAGCCCTGGCTGGTGTTCCCTTATGGCAGCTAGGTCCTGAGAGTAGGCATCTGGGAGTTCCATACATTGTTTTTCCGGGTAATGTTGGTGACGACAGAGCATTGGCGGAGGTTGTGAAGTCCTGGGCTCGTCCAGTCAGACTTTCATCAACCAAAGAGCTTCTTCTGAATGCAGAGAAGGGTGGATATGCTGTAGGAGCATTCAATGTATATAATTTGGAAGGAGTAGAGGCAGTTGTTGCTGCAGCAGAAGAAGAACAAAGTCCGGCTATATTACAGATCCATCCAGGTGCCTTGAAGCAAGGAGGGATCCCGTTGGTTGCATGTTGTATCGCTGCTGCAGAACAAGCCAGTGTTCCCATAACTGTACACTTTGACCACGGAACTTCAAAGCAAGATCTGGTGGAAGCTCTTGAGTTGGGATTTGATTCTGTCATGGCGGATGGTTCGCATCTTTCATTTACCGAGAATGTTTCATACACCAAGTTTGTCGCATTCTTTGCTCACTCAAAAGGCGTGCTAGTAGAAGCGGAACTAGGGAGATTGTCGGGGACAGAAGATGATTTAACTGTCGAAGATTATGAAGCAAGGCTGACTGATGTTAAGCAGGCTGAGGAGTTCATTGATAAAACCGGGATAGATGCTTTGGCAGTCTGTATTGGAAATGTGCACGGAAAATACCCTGCAAGTGGCCCGAATCTGAGACTCGATCTGCTTAAGGATCTGTACGCTTTGAGTTCGAAGAAAGGAGTCCTCCTGGTGCTTCATGGCGCTTCTGGTTTGTCGAAGGAACTTATAAAGGAATGCATAGAGCATGGTGTGAGGAAGTTCAATGTAAACACCGAGGTGCGAAAAGCTTACACGGATTCCCTTAACAACCCGAAGAAAGACCTGGTCCACGTTATGGCATCGGCGAAGGAAGCCATGAAAGCAGTGGTTGCAGAGAAGATGCATCTATTTGGTTCAGCAGGAAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1357_C DUF1537 F_bP_aldolase NAD_binding_11 NAD_binding_2

Protein Analysis

1427

Amino Acids

152.87

Weight (kDa)

6.13

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 65 - 215 3.7e-09 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 222 - 339 5.8e-17 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
NAD_binding_2 PF03446 375 - 534 1.3e-35 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 540 - 660 1e-28 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
SBD_N PF07005 707 - 944 4.6e-58 Sugar-binding N-terminal domain
NBD_C PF17042 969 - 1135 1.5e-34 Nucleotide-binding C-terminal domain
F_bP_aldolase PF01116 1150 - 1425 3.4e-94 Fructose-bisphosphate aldolase class-II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4097
Acc36I ACCTGC 2 cut(s) 1928, 2316
AccB1I GGYRCC 2 cut(s) 746, 3590
AccB7I CCANNNNNTGG 1 cut(s) 4204
AccII CGCG 2 cut(s) 493, 2583
AccIII TCCGGA 1 cut(s) 581
AcoI YGGCCR 4 cut(s) 611, 906, 2380, 2608
AcsI RAATTY 6 cut(s) 283, 1041, 1187, 1459, 1881, 2649
AcuI CTGAAG 2 cut(s) 1704, 2804
AcvI CACGTG 1 cut(s) 977
AcyI GRCGYC 1 cut(s) 176
AfaI GTAC 6 cut(s) 1540, 2314, 2418, 2490, 3669, 4028
AfeI AGCGCT 2 cut(s) 1336, 2795
AfiI CCNNNNNNNGG 7 cut(s) 337, 1487, 1512, 2253, 2527, 3989, 4204
AflII CTTAAG 3 cut(s) 1553, 2024, 4016
AflIII ACRYGT 1 cut(s) 491
AjnI CCWGG 9 cut(s) 1411, 1763, 2252, 2831, 3306, 3422, 3586, 4056, 4191
AjuI GAANNNNNNNTTGG 4 cut(s) 1014, 1046, 1451, 1483
AleI CACNNNNGTG 1 cut(s) 4145
Alw21I GWGCWC 3 cut(s) 1705, 2857, 3972
Alw44I GTGCAC 1 cut(s) 3968
AlwNI CAGNNNCTG 2 cut(s) 1931, 2675
Aor13HI TCCGGA 1 cut(s) 581
Aor51HI AGCGCT 2 cut(s) 1336, 2795
AoxI GGCC 8 cut(s) 328, 611, 906, 2256, 2380, 2608, 2666, 3991
ApaLI GTGCAC 1 cut(s) 3968
ApoI RAATTY 6 cut(s) 283, 1041, 1187, 1459, 1881, 2649
AseI ATTAAT 1 cut(s) 2285
Asp700I GAANNNNTTC 2 cut(s) 1855, 3244
AspLEI GCGC 5 cut(s) 27, 668, 1337, 2796, 4073
AspS9I GGNCC 8 cut(s) 329, 2187, 2256, 2509, 2666, 3335, 3992, 4195
AsuC2I CCSGG 2 cut(s) 3378, 3934
AsuHPI GGTGA 5 cut(s) 668, 1163, 1427, 2093, 3401
AsuII TTCGAA 2 cut(s) 1876, 4040
AsuNHI GCTAGC 1 cut(s) 2816
AvaII GGWCC 4 cut(s) 2187, 2509, 3335, 4195
AxyI CCTNAGG 1 cut(s) 1077
BaeGI GKGCMC 2 cut(s) 1519, 3972
BaeI ACNNNNGTAYC 2 cut(s) 426, 459
BalI TGGCCA 2 cut(s) 908, 2382
BamHI GGATCC 1 cut(s) 3609
BanI GGYRCC 2 cut(s) 746, 3590
BanII GRGCYC 3 cut(s) 1705, 2857, 3430
BbrPI CACGTG 1 cut(s) 977
BbsI GAAGAC 1 cut(s) 2032
Bbv12I GWGCWC 3 cut(s) 1705, 2857, 3972
BbvCI CCTCAGC 1 cut(s) 3912
BceAI ACGGC 1 cut(s) 626
BcgI CGANNNNNNTGC 2 cut(s) 2414, 2448
BciT130I CCWGG 9 cut(s) 1413, 1765, 2254, 2833, 3308, 3424, 3588, 4058, 4193
BciVI GTATCC 2 cut(s) 427, 2524
BclI TGATCA 1 cut(s) 2769
BcnI CCSGG 2 cut(s) 3378, 3934
BfaI CTAG 7 cut(s) 1430, 2733, 2817, 2876, 3332, 3821, 3836
BfmI CTRYAG 8 cut(s) 1935, 2316, 2523, 2963, 3024, 3492, 3543, 3639
BfoI RGCGCY 4 cut(s) 669, 1338, 2797, 4074
BfrI CTTAAG 3 cut(s) 1553, 2024, 4016
BfuAI ACCTGC 2 cut(s) 1928, 2316
BfuI GTATCC 2 cut(s) 427, 2524
BglII AGATCT 1 cut(s) 3697
BlpI GCTNAGC 1 cut(s) 917
BmcAI AGTACT 1 cut(s) 2418
Bme18I GGWCC 4 cut(s) 2187, 2509, 3335, 4195
BmgT120I GGNCC 8 cut(s) 329, 2187, 2256, 2509, 2666, 3335, 3992, 4195
BmiI GGNNCC 4 cut(s) 316, 748, 3592, 3611
BmrI ACTGGG 2 cut(s) 2048, 2429
BmtI GCTAGC 1 cut(s) 2820
BmuI ACTGGG 2 cut(s) 2048, 2429
BpiI GAAGAC 1 cut(s) 2032
BpmI CTGGAG 4 cut(s) 1251, 1398, 1680, 2236
Bpu10I CCTNAGC 1 cut(s) 3912
Bpu1102I GCTNAGC 1 cut(s) 917
Bpu14I TTCGAA 2 cut(s) 1876, 4040
BpuEI CTTGAG 8 cut(s) 58, 235, 722, 907, 1308, 1845, 2281, 3734
BpuMI CCSGG 2 cut(s) 3378, 3934
BsaAI YACGTR 1 cut(s) 977
BsaBI GATNNNNATC 2 cut(s) 569, 2136
BsaHI GRCGYC 1 cut(s) 176
BsaI GGTCTC 2 cut(s) 975, 2909
BsaJI CCNNGG 9 cut(s) 180, 767, 1509, 2253, 2868, 3306, 3423, 3679, 4143
BsaWI WCCGGW 5 cut(s) 100, 581, 1535, 2017, 3128
BsaXI ACNNNNNCTCC 2 cut(s) 1393, 1423
Bsc4I CCNNNNNNNGG 7 cut(s) 337, 1487, 1512, 2253, 2527, 3989, 4204
Bse118I RCCGGY 2 cut(s) 755, 2606
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 4 cut(s) 177, 633, 1155, 1695
Bse8I GATNNNNATC 2 cut(s) 569, 2136
BseAI TCCGGA 1 cut(s) 581
BseBI CCWGG 9 cut(s) 1413, 1765, 2254, 2833, 3308, 3424, 3588, 4058, 4193
BseDI CCNNGG 9 cut(s) 180, 767, 1509, 2253, 2868, 3306, 3423, 3679, 4143
BseJI GATNNNNATC 2 cut(s) 569, 2136
BseLI CCNNNNNNNGG 7 cut(s) 337, 1487, 1512, 2253, 2527, 3989, 4204
BseMI GCAATG 4 cut(s) 177, 633, 1155, 1695
BseRI GAGGAG 5 cut(s) 230, 350, 2609, 3053, 3929
BseSI GKGCMC 2 cut(s) 1519, 3972
BseX3I CGGCCG 1 cut(s) 2608
BsgI GTGCAG 2 cut(s) 1587, 1806
Bsh1236I CGCG 2 cut(s) 493, 2583
Bsh1285I CGRYCG 1 cut(s) 2611
BshFI GGCC 8 cut(s) 330, 613, 908, 2258, 2382, 2610, 2668, 3993
BshNI GGYRCC 2 cut(s) 746, 3590
BsiEI CGRYCG 1 cut(s) 2611
BsiHKAI GWGCWC 3 cut(s) 1705, 2857, 3972
BslFI GGGAC 2 cut(s) 3312, 3865
BslI CCNNNNNNNGG 7 cut(s) 337, 1487, 1512, 2253, 2527, 3989, 4204
BsmBI CGTCTC 2 cut(s) 40, 3190
BsmFI GGGAC 2 cut(s) 3312, 3865
BsmI GAATGC 6 cut(s) 1816, 2341, 3475, 3500, 3794, 4109
BsnI GGCC 8 cut(s) 330, 613, 908, 2258, 2382, 2610, 2668, 3993
Bso31I GGTCTC 2 cut(s) 975, 2909
Bsp119I TTCGAA 2 cut(s) 1876, 4040
Bsp1286I GDGCHC 5 cut(s) 1519, 1705, 2857, 3430, 3972
Bsp13I TCCGGA 1 cut(s) 581
Bsp1407I TGTACA 2 cut(s) 2488, 3667
Bsp1720I GCTNAGC 1 cut(s) 917
Bsp19I CCATGG 1 cut(s) 1509
Bsp68I TCGCGA 1 cut(s) 2583
BspANI GGCC 8 cut(s) 330, 613, 908, 2258, 2382, 2610, 2668, 3993
BspEI TCCGGA 1 cut(s) 581
BspFNI CGCG 2 cut(s) 493, 2583
BspHI TCATGA 2 cut(s) 567, 2161
BspLI GGNNCC 4 cut(s) 316, 748, 3592, 3611
BspMAI CTGCAG 5 cut(s) 1939, 2320, 2527, 3547, 3643
BspMI ACCTGC 2 cut(s) 1928, 2316
BspOI GCTAGC 1 cut(s) 2820
BspQI GCTCTTC 1 cut(s) 2946
BspT104I TTCGAA 2 cut(s) 1876, 4040
BspT107I GGYRCC 2 cut(s) 746, 3590
BspTI CTTAAG 3 cut(s) 1553, 2024, 4016
BspTNI GGTCTC 2 cut(s) 975, 2909
BsrDI GCAATG 4 cut(s) 177, 633, 1155, 1695
BsrFI RCCGGY 2 cut(s) 755, 2606
BsrGI TGTACA 2 cut(s) 2488, 3667
BssAI RCCGGY 2 cut(s) 755, 2606
BssECI CCNNGG 9 cut(s) 180, 767, 1509, 2253, 2868, 3306, 3423, 3679, 4143
BssNI GRCGYC 1 cut(s) 176
BssT1I CCWWGG 3 cut(s) 180, 1509, 2868
Bst2UI CCWGG 9 cut(s) 1413, 1765, 2254, 2833, 3308, 3424, 3588, 4058, 4193
Bst6I CTCTTC 3 cut(s) 1922, 2946, 3024
BstACI GRCGYC 1 cut(s) 176
BstAFI CTTAAG 3 cut(s) 1553, 2024, 4016
BstAPI GCANNNNNTGC 5 cut(s) 1058, 3284, 3539, 3800, 4238
BstAUI TGTACA 2 cut(s) 2488, 3667
BstBAI YACGTR 1 cut(s) 977
BstBI TTCGAA 2 cut(s) 1876, 4040
BstC8I GCNNGC 7 cut(s) 1090, 1656, 2608, 2792, 2818, 3818, 3909
BstDSI CCRYGG 3 cut(s) 767, 1509, 3679
BstFNI CGCG 2 cut(s) 493, 2583
BstH2I RGCGCY 4 cut(s) 669, 1338, 2797, 4074
BstHHI GCGC 5 cut(s) 27, 668, 1337, 2796, 4073
BstMCI CGRYCG 1 cut(s) 2611
BstNI CCWGG 9 cut(s) 1413, 1765, 2254, 2833, 3308, 3424, 3588, 4058, 4193
BstNSI RCATGY 3 cut(s) 1795, 2718, 3627
BstSFI CTRYAG 8 cut(s) 1935, 2316, 2523, 2963, 3024, 3492, 3543, 3639
BstSLI GKGCMC 2 cut(s) 1519, 3972
BstUI CGCG 2 cut(s) 493, 2583
BstV2I GAAGAC 1 cut(s) 2032
BstX2I RGATCY 8 cut(s) 901, 1843, 2071, 2872, 3579, 3609, 3697, 4021
BstXI CCANNNNNNTGG 1 cut(s) 2061
BstYI RGATCY 8 cut(s) 901, 1843, 2071, 2872, 3579, 3609, 3697, 4021
BstZI CGGCCG 1 cut(s) 2608
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 2 cut(s) 427, 2524
BsuRI GGCC 8 cut(s) 330, 613, 908, 2258, 2382, 2610, 2668, 3993
BtgI CCRYGG 3 cut(s) 767, 1509, 3679
BtsI GCAGTG 3 cut(s) 253, 2808, 4239
BtuMI TCGCGA 1 cut(s) 2583
BveI ACCTGC 2 cut(s) 1928, 2316
Cac8I GCNNGC 7 cut(s) 1090, 1656, 2608, 2792, 2818, 3818, 3909
CaiI CAGNNNCTG 2 cut(s) 1931, 2675
CciI TCATGA 2 cut(s) 567, 2161
CfoI GCGC 5 cut(s) 27, 668, 1337, 2796, 4073
Cfr10I RCCGGY 2 cut(s) 755, 2606
Cfr13I GGNCC 8 cut(s) 329, 2187, 2256, 2509, 2666, 3335, 3992, 4195
CseI GACGC 6 cut(s) 165, 499, 602, 1662, 2675, 3207
CsiI ACCWGGT 1 cut(s) 4191
Csp6I GTAC 6 cut(s) 1539, 2313, 2417, 2489, 3668, 4027
CspCI CAANNNNNGTGG 6 cut(s) 1228, 1263, 2928, 2963, 3276, 3311
CviQI GTAC 6 cut(s) 1539, 2313, 2417, 2489, 3668, 4027
DraI TTTAAA 3 cut(s) 303, 603, 1179
EaeI YGGCCR 4 cut(s) 611, 906, 2380, 2608
EagI CGGCCG 1 cut(s) 2608
Eam1104I CTCTTC 3 cut(s) 1922, 2946, 3024
EarI CTCTTC 3 cut(s) 1922, 2946, 3024
EciI GGCGGA 3 cut(s) 2792, 3422, 3755
Ecl136II GAGCTC 2 cut(s) 1703, 2855
EclXI CGGCCG 1 cut(s) 2608
Eco130I CCWWGG 3 cut(s) 180, 1509, 2868
Eco24I GRGCYC 3 cut(s) 1705, 2857, 3430
Eco31I GGTCTC 2 cut(s) 975, 2909
Eco32I GATATC 1 cut(s) 784
Eco47I GGWCC 4 cut(s) 2187, 2509, 3335, 4195
Eco47III AGCGCT 2 cut(s) 1336, 2795
Eco52I CGGCCG 1 cut(s) 2608
Eco53kI GAGCTC 2 cut(s) 1703, 2855
Eco57I CTGAAG 2 cut(s) 1704, 2804
Eco72I CACGTG 1 cut(s) 977
Eco81I CCTNAGG 1 cut(s) 1077
EcoICRI GAGCTC 2 cut(s) 1703, 2855
EcoO109I RGGNCCY 2 cut(s) 2256, 3335
EcoRII CCWGG 9 cut(s) 1411, 1763, 2252, 2831, 3306, 3422, 3586, 4056, 4191
EcoRV GATATC 1 cut(s) 784
EcoT14I CCWWGG 3 cut(s) 180, 1509, 2868
EcoT22I ATGCAT 4 cut(s) 2437, 2720, 4109, 4254
EcoT38I GRGCYC 3 cut(s) 1705, 2857, 3430
ErhI CCWWGG 3 cut(s) 180, 1509, 2868
Esp3I CGTCTC 2 cut(s) 40, 3190
FalI AAGNNNNNCTT 6 cut(s) 1998, 2030, 2783, 2815, 3447, 3479
FaqI GGGAC 2 cut(s) 3312, 3865
FauI CCCGC 2 cut(s) 793, 939
FauNDI CATATG 1 cut(s) 2923
FbaI TGATCA 1 cut(s) 2769
FriOI GRGCYC 3 cut(s) 1705, 2857, 3430
FspBI CTAG 7 cut(s) 1430, 2733, 2817, 2876, 3332, 3821, 3836
GlaI GCGC 5 cut(s) 26, 667, 1336, 2795, 4072
GsuI CTGGAG 4 cut(s) 1251, 1398, 1680, 2236
HaeII RGCGCY 4 cut(s) 669, 1338, 2797, 4074
HaeIII GGCC 8 cut(s) 330, 613, 908, 2258, 2382, 2610, 2668, 3993
HgaI GACGC 6 cut(s) 165, 499, 602, 1662, 2675, 3207
HhaI GCGC 5 cut(s) 27, 668, 1337, 2796, 4073
Hin1I GRCGYC 1 cut(s) 176
Hin6I GCGC 5 cut(s) 25, 666, 1335, 2794, 4071
HinP1I GCGC 5 cut(s) 25, 666, 1335, 2794, 4071
HincII GTYRAC 2 cut(s) 1120, 2725
HindII GTYRAC 2 cut(s) 1120, 2725
HindIII AAGCTT 4 cut(s) 264, 638, 1592, 4155
HpaI GTTAAC 1 cut(s) 2725
HphI GGTGA 5 cut(s) 668, 1163, 1427, 2093, 3401
Hpy166II GTNNAC 9 cut(s) 80, 1120, 2305, 2491, 2725, 3670, 3970, 4138, 4198
Hpy8I GTNNAC 9 cut(s) 80, 1120, 2305, 2491, 2725, 3670, 3970, 4138, 4198
Hpy99I CGWCG 3 cut(s) 37, 203, 1678
HpyCH4IV ACGT 7 cut(s) 41, 49, 976, 1727, 2034, 3217, 4200
HpySE526I ACGT 7 cut(s) 41, 49, 976, 1727, 2034, 3217, 4200
Hsp92I GRCGYC 1 cut(s) 176
HspAI GCGC 5 cut(s) 25, 666, 1335, 2794, 4071
Kpn2I TCCGGA 1 cut(s) 581
KroI GCCGGC 1 cut(s) 2606
KroNI GCCGGC 1 cut(s) 2608
Ksp22I TGATCA 1 cut(s) 2769
KspAI GTTAAC 1 cut(s) 2725
LguI GCTCTTC 1 cut(s) 2946
MabI ACCWGGT 1 cut(s) 4191
MaeI CTAG 7 cut(s) 1430, 2733, 2817, 2876, 3332, 3821, 3836
MaeII ACGT 7 cut(s) 41, 49, 976, 1727, 2034, 3217, 4200
MaeIII GTNAC 7 cut(s) 110, 977, 1309, 2629, 2735, 3218, 3389
MfeI CAATTG 1 cut(s) 1649
MflI RGATCY 8 cut(s) 901, 1843, 2071, 2872, 3579, 3609, 3697, 4021
MhlI GDGCHC 5 cut(s) 1519, 1705, 2857, 3430, 3972
MlsI TGGCCA 2 cut(s) 908, 2382
MluI ACGCGT 1 cut(s) 491
MluNI TGGCCA 2 cut(s) 908, 2382
MlyI GAGTC 4 cut(s) 60, 1222, 3999, 4059
MmeI TCCRAC 3 cut(s) 1108, 2165, 2461
Mox20I TGGCCA 2 cut(s) 908, 2382
Mph1103I ATGCAT 4 cut(s) 2437, 2720, 4109, 4254
MroI TCCGGA 1 cut(s) 581
MroNI GCCGGC 1 cut(s) 2606
MroXI GAANNNNTTC 2 cut(s) 1855, 3244
MscI TGGCCA 2 cut(s) 908, 2382
MslI CAYNNNNRTG 2 cut(s) 3588, 4145
Msp20I TGGCCA 2 cut(s) 908, 2382
MspA1I CMGCKG 4 cut(s) 509, 2408, 2762, 3059
MspCI CTTAAG 3 cut(s) 1553, 2024, 4016
MunI CAATTG 1 cut(s) 1649
Mva1269I GAATGC 6 cut(s) 1816, 2341, 3475, 3500, 3794, 4109
MvaI CCWGG 9 cut(s) 1413, 1765, 2254, 2833, 3308, 3424, 3588, 4058, 4193
MvnI CGCG 2 cut(s) 493, 2583
NaeI GCCGGC 1 cut(s) 2608
NciI CCSGG 2 cut(s) 3378, 3934
NcoI CCATGG 1 cut(s) 1509
NdeI CATATG 1 cut(s) 2923
NgoMIV GCCGGC 1 cut(s) 2606
NheI GCTAGC 1 cut(s) 2816
NlaIV GGNNCC 4 cut(s) 316, 748, 3592, 3611
NmeAIII GCCGAG 1 cut(s) 3155
NmuCI GTSAC 5 cut(s) 110, 977, 1309, 2735, 3389
NruI TCGCGA 1 cut(s) 2583
NsiI ATGCAT 4 cut(s) 2437, 2720, 4109, 4254
NspI RCATGY 3 cut(s) 1795, 2718, 3627
NspV TTCGAA 2 cut(s) 1876, 4040
OliI CACNNNNGTG 1 cut(s) 4145
PacI TTAATTAA 1 cut(s) 2289
PagI TCATGA 2 cut(s) 567, 2161
PciSI GCTCTTC 1 cut(s) 2946
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PctI GAATGC 6 cut(s) 1816, 2341, 3475, 3500, 3794, 4109
PdiI GCCGGC 1 cut(s) 2608
PdmI GAANNNNTTC 2 cut(s) 1855, 3244
PflFI GACNNNGTC 2 cut(s) 2036, 4193
PflMI CCANNNNNTGG 1 cut(s) 4204
PfoI TCCNGGA 2 cut(s) 1411, 1763
PleI GAGTC 4 cut(s) 60, 1222, 3999, 4058
PmaCI CACGTG 1 cut(s) 977
PmlI CACGTG 1 cut(s) 977
PpsI GAGTC 4 cut(s) 60, 1222, 3999, 4058
Ppu21I YACGTR 1 cut(s) 977
PpuMI RGGWCCY 1 cut(s) 3335
PshBI ATTAAT 1 cut(s) 2285
PsiI TTATAA 1 cut(s) 4097
Psp124BI GAGCTC 2 cut(s) 1705, 2857
Psp5II RGGWCCY 1 cut(s) 3335
Psp6I CCWGG 9 cut(s) 1411, 1763, 2252, 2831, 3306, 3422, 3586, 4056, 4191
PspCI CACGTG 1 cut(s) 977
PspGI CCWGG 9 cut(s) 1411, 1763, 2252, 2831, 3306, 3422, 3586, 4056, 4191
PspN4I GGNNCC 4 cut(s) 316, 748, 3592, 3611
PspPI GGNCC 8 cut(s) 329, 2187, 2256, 2509, 2666, 3335, 3992, 4195
PspPPI RGGWCCY 1 cut(s) 3335
PstI CTGCAG 5 cut(s) 1939, 2320, 2527, 3547, 3643
PstNI CAGNNNCTG 2 cut(s) 1931, 2675
PsuI RGATCY 8 cut(s) 901, 1843, 2071, 2872, 3579, 3609, 3697, 4021
PsyI GACNNNGTC 2 cut(s) 2036, 4193
PvuII CAGCTG 3 cut(s) 509, 2762, 3059
RruI TCGCGA 1 cut(s) 2583
RsaI GTAC 6 cut(s) 1540, 2314, 2418, 2490, 3669, 4028
RsaNI GTAC 6 cut(s) 1539, 2313, 2417, 2489, 3668, 4027
RseI CAYNNNNRTG 2 cut(s) 3588, 4145
SacI GAGCTC 2 cut(s) 1705, 2857
SapI GCTCTTC 1 cut(s) 2946
Sau96I GGNCC 8 cut(s) 329, 2187, 2256, 2509, 2666, 3335, 3992, 4195
SbfI CCTGCAGG 1 cut(s) 2527
ScaI AGTACT 1 cut(s) 2418
SchI GAGTC 4 cut(s) 60, 1222, 3999, 4059
SdaI CCTGCAGG 1 cut(s) 2527
SduI GDGCHC 5 cut(s) 1519, 1705, 2857, 3430, 3972
SexAI ACCWGGT 1 cut(s) 4191
SfcI CTRYAG 8 cut(s) 1935, 2316, 2523, 2963, 3024, 3492, 3543, 3639
SfuI TTCGAA 2 cut(s) 1876, 4040
SinI GGWCC 4 cut(s) 2187, 2509, 3335, 4195
SmiMI CAYNNNNRTG 2 cut(s) 3588, 4145
Sse8387I CCTGCAGG 1 cut(s) 2527
SspI AATATT 1 cut(s) 462
SspMI CTAG 7 cut(s) 1430, 2733, 2817, 2876, 3332, 3821, 3836
SstI GAGCTC 2 cut(s) 1705, 2857
StyI CCWWGG 3 cut(s) 180, 1509, 2868
TaiI ACGT 7 cut(s) 44, 52, 979, 1730, 2037, 3220, 4203
TatI WGTACW 3 cut(s) 2416, 2488, 3667
TauI GCSGC 3 cut(s) 330, 2408, 2613
TseFI GTSAC 5 cut(s) 110, 977, 1309, 2735, 3389
Tsp45I GTSAC 5 cut(s) 110, 977, 1309, 2735, 3389
TspGWI ACGGA 8 cut(s) 49, 50, 756, 2195, 2913, 3696, 3987, 4178
Tth111I GACNNNGTC 2 cut(s) 2036, 4193
Van91I CCANNNNNTGG 1 cut(s) 4204
Vha464I CTTAAG 3 cut(s) 1553, 2024, 4016
VneI GTGCAC 1 cut(s) 3968
VpaK11BI GGWCC 4 cut(s) 2187, 2509, 3335, 4195
VspI ATTAAT 1 cut(s) 2285
XapI RAATTY 6 cut(s) 283, 1041, 1187, 1459, 1881, 2649
XceI RCATGY 3 cut(s) 1795, 2718, 3627
XcmI CCANNNNNNNNNTGG 1 cut(s) 4231
XmnI GAANNNNTTC 2 cut(s) 1855, 3244
XspI CTAG 7 cut(s) 1430, 2733, 2817, 2876, 3332, 3821, 3836
ZrmI AGTACT 1 cut(s) 2418
Zsp2I ATGCAT 4 cut(s) 2437, 2720, 4109, 4254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.