pycom14g20130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011123
Physical Location & Seq
Forward (+)
21238971 .. 21240779
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
ATGGGGTTGCAGTGGATGATATTGACGTACGTGGTGGCGGCAGAGGCGGCGTTGTCCTTTCTCCTAACCATTCCGGCGCCCAAGCTGTTGAAGAATCGATTCGTGTCGCTGGTCTCTCTAATCCTCCAACCCGCCCTCTTCATCGTCCCCTTCGCCGGATTTCAGCTCCTGGATATATACTGGAAGAATGAGCACCGGTTGGCCTGCACCTCTGATATCTGCACTGCCTCTGAGCGTGACCGCTATGAGAAATCGATGTACAAGGCTCAAAGGAATGTAATTCTTTGTGCTTCAGCATGTCTGCTTTACTGGTGTCTATTTCGCATCTGTAAATTGTGCAAGGACATTCAGAACTTGGAGGAAGTGGAGAAGAGGTACAAGGACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bap31

Protein Analysis

130

Amino Acids

14.99

Weight (kDa)

8.86

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bap31 PF05529 1 - 122 2.3e-06 Bap31/Bap29 transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 4 cut(s) 38, 47, 132, 241
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 3 cut(s) 29, 260, 377
AfiI CCNNNNNNNGG 1 cut(s) 155
AgeI ACCGGT 1 cut(s) 195
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 2 cut(s) 85, 166
AluI AGCT 2 cut(s) 85, 166
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 118
AlwNI CAGNNNCTG 1 cut(s) 169
AoxI GGCC 1 cut(s) 201
AsiGI ACCGGT 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 79
BanI GGYRCC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 195
BciT130I CCWGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 2 cut(s) 39, 48
BlsI GCNGC 2 cut(s) 40, 49
Bme1390I CCNGG 1 cut(s) 170
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 333
Bsa29I ATCGAT 2 cut(s) 97, 254
BsaAI YACGTR 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 2 cut(s) 185, 314
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 97, 254
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 185, 314
BsgI GTGCAG 2 cut(s) 190, 205
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 76
BshTI ACCGGT 1 cut(s) 195
BshVI ATCGAT 2 cut(s) 97, 254
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 3 cut(s) 74, 156, 196
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 1 cut(s) 203
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 258
BspACI CCGC 4 cut(s) 38, 47, 132, 241
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 2 cut(s) 97, 254
BspLI GGNNCC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 195
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 185, 314
BssAI RCCGGY 1 cut(s) 195
BssNI GRCGYC 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 170
Bst6I CTCTTC 2 cut(s) 143, 365
BstACI GRCGYC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 231
BstF5I GGATG 1 cut(s) 21
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 2 cut(s) 44, 47
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 168
Bsu15I ATCGAT 2 cut(s) 97, 254
BsuRI GGCC 1 cut(s) 203
BsuTUI ATCGAT 2 cut(s) 97, 254
BtsCI GGATG 1 cut(s) 21
BtsI GCAGTG 2 cut(s) 17, 222
BtsIMutI CAGTG 2 cut(s) 17, 222
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 169
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 195
ClaI ATCGAT 2 cut(s) 97, 254
Csp6I GTAC 3 cut(s) 28, 259, 376
CspAI ACCGGT 1 cut(s) 195
CviAII CATG 1 cut(s) 297
CviJI RGCY 4 cut(s) 85, 166, 203, 266
CviKI_1 RGCY 4 cut(s) 85, 166, 203, 266
CviQI GTAC 3 cut(s) 28, 259, 376
DdeI CTNAG 1 cut(s) 231
DinI GGCGCC 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 143, 365
EarI CTCTTC 2 cut(s) 143, 365
Eco31I GGTCTC 1 cut(s) 118
Eco32I GATATC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 168
EcoRV GATATC 1 cut(s) 217
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
FaeI CATG 1 cut(s) 300
FaiI YATR 4 cut(s) 176, 178, 246, 298
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 296
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 39, 48
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 2 cut(s) 39, 48
GlaI GCGC 1 cut(s) 78
GluI GCNGC 2 cut(s) 39, 48
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 1 cut(s) 203
HapII CCGG 3 cut(s) 74, 156, 196
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 300
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 2 cut(s) 94, 99
HpaII CCGG 3 cut(s) 74, 156, 196
Hpy188I TCNGA 3 cut(s) 214, 232, 351
HpyAV CCTTC 1 cut(s) 160
HpyCH4IV ACGT 2 cut(s) 26, 30
HpyCH4V TGCA 4 cut(s) 10, 207, 222, 339
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 47
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 2 cut(s) 26, 30
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 300
HspAI GCGC 1 cut(s) 77
KasI GGCGCC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 9 cut(s) 87, 95, 155, 166, 169, 182, 209, 217, 295
LweI GCATC 1 cut(s) 333
MaeII ACGT 2 cut(s) 26, 30
MaeIII GTNAC 1 cut(s) 236
MboII GAAGA 4 cut(s) 103, 130, 196, 382
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 2 cut(s) 279, 332
Mly113I GGCGCC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 7 cut(s) 37, 134, 146, 220, 238, 352, 366
MroXI GAANNNNTTC 1 cut(s) 98
MspI CCGG 3 cut(s) 74, 156, 196
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 2 cut(s) 44, 47
NarI GGCGCC 1 cut(s) 77
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 94, 99
Pfl23II CGTACG 1 cut(s) 27
PfoI TCCNGGA 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 2 cut(s) 40, 49
PluTI GGCGCC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 78
PstNI CAGNNNCTG 1 cut(s) 169
RsaI GTAC 3 cut(s) 29, 260, 377
RsaNI GTAC 3 cut(s) 28, 259, 376
SatI GCNGC 2 cut(s) 39, 48
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 29, 33, 87, 168, 212, 377
SfaNI GCATC 1 cut(s) 333
SfoI GGCGCC 1 cut(s) 78
Sse9I AATT 2 cut(s) 279, 332
SsiI CCGC 4 cut(s) 38, 47, 132, 241
SspDI GGCGCC 1 cut(s) 76
StyD4I CCNGG 1 cut(s) 168
TaiI ACGT 2 cut(s) 29, 33
TaqI TCGA 2 cut(s) 97, 254
TasI AATT 2 cut(s) 279, 332
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 41, 50
TfiI GAWTC 2 cut(s) 94, 99
TscAI CASTG 2 cut(s) 17, 229
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 130
TspRI CASTG 2 cut(s) 17, 229
XceI RCATGY 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.