pycom14g20550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0017160
Physical Location & Seq
Forward (+)
21575537 .. 21575917
381 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGTCGGATCCATTCGAGAGAGCGAAAGGAGGCAGATTGACCTTCAAAGACGGAACTCTCGCCACTCGCAGCAAAGCCATCGACAAGAAGAAGAAGAAAAAGAAGAAACAGCAGCTTCAGGAGCAGCATATGTACCCTAACGACGATCCCCAAGTGGTGGCTGAGGACGGAAGCGCCGAAGCAGCAGCAGCAGTAGTAGAAGGAACTGGAGGAGGAACAGAGACGTACACCATCGACGCAGCGAAGCGGATGAAGTATGAGCAGCTGTTCCCGGTTGAGGCCAAGAAGTTCGGCTACGATCCCAAATCCGTTGAACGGAAGTCCGTCGAGGACGTTCTTGACGACCGCGTCAAGAAGAAAGCCGACCGCTACTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

127

Amino Acids

14.15

Weight (kDa)

9.3

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25720
fragaria_vesca FvH4_5g18200
malus_domestica MD14G1244400.v1.1
prunus_persica Prupe.5G241900_v2.0.a1
pyrus_communis pycom14g20550
rosa_chinensis RchiOBHm_Chr7g0177391
rosa_laevigata RLG00000005540
rosa_multiflora Rmu_sc0005545.1_g000002
rosa_rugosa Rorug06G0405500
rosa_samantha Rh7AG005200 Rh7BG005200 Rh7CG005300
rosa_wichuraiana Rw7G000430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 157
AccII CGCG 1 cut(s) 348
AciI CCGC 3 cut(s) 247, 346, 367
AclWI GGATC 4 cut(s) 2, 15, 140, 293
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 2 cut(s) 134, 227
AfiI CCNNNNNNNGG 3 cut(s) 157, 277, 315
AgsI TTSAA 2 cut(s) 46, 314
AluBI AGCT 2 cut(s) 115, 265
AluI AGCT 2 cut(s) 115, 265
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 4 cut(s) 2, 15, 140, 293
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 8 cut(s) 69, 112, 124, 182, 185, 188, 239, 262
AspLEI GCGC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 272
BamHI GGATCC 1 cut(s) 7
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 8 cut(s) 81, 124, 136, 194, 197, 200, 251, 274
BccI CCATC 2 cut(s) 86, 239
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 215
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 8 cut(s) 70, 113, 125, 183, 186, 189, 240, 263
BlsI GCNGC 8 cut(s) 71, 114, 126, 184, 187, 190, 241, 264
Bme1390I CCNGG 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 272
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 277, 315
Bse1I ACTGG 1 cut(s) 211
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 3 cut(s) 157, 277, 315
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 1 cut(s) 225
BseXI GCAGC 8 cut(s) 81, 124, 136, 194, 197, 200, 251, 274
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 2 cut(s) 346, 367
BshFI GGCC 1 cut(s) 281
BsiEI CGRYCG 2 cut(s) 346, 367
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 157, 277, 315
BsmAI GTCTC 1 cut(s) 215
BsmBI CGTCTC 1 cut(s) 215
BsnI GGCC 1 cut(s) 281
Bsp143I GATC 3 cut(s) 7, 145, 298
BspACI CCGC 3 cut(s) 247, 346, 367
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 4 cut(s) 2, 15, 140, 293
BsrI ACTGG 1 cut(s) 211
BssMI GATC 3 cut(s) 7, 145, 298
Bst4CI ACNGT 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 3 cut(s) 10, 148, 301
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 3 cut(s) 7, 145, 298
BstMCI CGRYCG 2 cut(s) 346, 367
BstMWI GCNNNNNNNGC 3 cut(s) 121, 182, 188
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 8 cut(s) 81, 124, 136, 194, 197, 200, 251, 274
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 281
BtsCI GGATG 1 cut(s) 255
CfoI GCGC 1 cut(s) 176
CseI GACGC 2 cut(s) 245, 337
Csp6I GTAC 2 cut(s) 133, 226
CviJI RGCY 7 cut(s) 77, 115, 161, 265, 281, 294, 362
CviKI_1 RGCY 7 cut(s) 77, 115, 161, 265, 281, 294, 362
CviQI GTAC 2 cut(s) 133, 226
DdeI CTNAG 1 cut(s) 162
DpnI GATC 3 cut(s) 9, 147, 300
DpnII GATC 3 cut(s) 7, 145, 298
DrdI GACNNNNNNGTC 1 cut(s) 347
DseDI GACNNNNNNGTC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 101
Esp3I CGTCTC 1 cut(s) 215
FaiI YATR 3 cut(s) 129, 131, 258
FauNDI CATATG 1 cut(s) 129
Fnu4HI GCNGC 8 cut(s) 70, 113, 125, 183, 186, 189, 240, 263
FokI GGATG 1 cut(s) 262
Fsp4HI GCNGC 8 cut(s) 70, 113, 125, 183, 186, 189, 240, 263
GlaI GCGC 1 cut(s) 175
GluI GCNGC 8 cut(s) 70, 113, 125, 183, 186, 189, 240, 263
GsuI CTGGAG 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 1 cut(s) 281
HapII CCGG 1 cut(s) 272
HgaI GACGC 2 cut(s) 245, 337
HhaI GCGC 1 cut(s) 176
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HpaII CCGG 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 4 cut(s) 16, 119, 338, 352
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 3 cut(s) 146, 239, 329
HpyAV CCTTC 2 cut(s) 52, 194
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 2 cut(s) 224, 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 182, 188
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 2 cut(s) 224, 333
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 3 cut(s) 7, 145, 298
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 3 cut(s) 104, 192, 285
Lsp1109I GCAGC 8 cut(s) 81, 124, 136, 194, 197, 200, 251, 274
MaeII ACGT 2 cut(s) 224, 333
MalI GATC 3 cut(s) 9, 147, 300
MboI GATC 3 cut(s) 7, 145, 298
MboII GAAGA 5 cut(s) 100, 103, 106, 115, 367
MflI RGATCY 1 cut(s) 7
MnlI CCTC 6 cut(s) 23, 157, 203, 206, 271, 322
MspA1I CMGCKG 1 cut(s) 265
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 121, 182, 188
NciI CCSGG 1 cut(s) 272
NdeI CATATG 1 cut(s) 129
NdeII GATC 3 cut(s) 7, 145, 298
NlaIV GGNNCC 1 cut(s) 9
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 339
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 8 cut(s) 71, 114, 126, 184, 187, 190, 241, 264
PspN4I GGNNCC 1 cut(s) 9
PsuI RGATCY 1 cut(s) 7
PsyI GACNNNGTC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 134, 227
RsaNI GTAC 2 cut(s) 133, 226
SatI GCNGC 8 cut(s) 70, 113, 125, 183, 186, 189, 240, 263
Sau3AI GATC 3 cut(s) 7, 145, 298
ScrFI CCNGG 1 cut(s) 272
SetI ASST 5 cut(s) 44, 117, 227, 267, 336
SsiI CCGC 3 cut(s) 247, 346, 367
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 2 cut(s) 227, 336
TaqI TCGA 4 cut(s) 15, 81, 234, 327
TseI GCWGC 8 cut(s) 69, 112, 124, 182, 185, 188, 239, 262
TspDTI ATGAA 1 cut(s) 266
TspGWI ACGGA 5 cut(s) 66, 183, 298, 313, 331
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.