pycom14g20630

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009074
Physical Location & Seq
Reverse (-)
21655773 .. 21658016
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGTCGTCCTCGGGCGGCCACTCCGGCAGTGTTGGCGGTCCGATCGCCCCTCAGCTTTACTTCTGCCACCAGTGCGACCGTACGGTTTCCCTCACCCCCTCACCTACCTCCGATCTCGTTTGCCCGCTTTGCAGCGGCGGCTTCCTCGAAGAGATGGAAACCCCCAACCCTAACTCTTTCCCCCCCAATCCTTTCTTCTCCTTCCCCTCCTCCGACGCCCCTCCCTTCGCCTCCAGCGGATTTCCCCTCCTCTTCTCCTCCTCCGCCTCTGGTCCCGCTCCCGCTCCCGCCCCTGCCAATCACGCCGATGATCTCTCCTCCCTCTTCGGTGGCGCACCCTCACGCTCCTCACCCTTTCACGACCCCGACGCCTTCAACCCGTTCGTCTTCCTCCAGAACTACTTGCAAACCCTAAGGGCAAATGGTGCCAATGTCCAGTTCGTGATCGATGGCAGCCCTGGCGGCGGCGGCGGCGGGGACCCATCAGGTTTTCGTGCTCCTACCAATCTTAATCTCGGCGATTACTTCTTTGGCCCGGGCATCGAGCAATTGATCCAACAATTAGCCGAGAACGATCCAAACAGGTACGGCACCCCACCCGCCTCTAAATCCGCCATCGTAGCCTTGCCGGTTGTGAAAATTTCAGAGGAGTTGTTGGCTTCGGATTCATCACAGTGCGCGGTGTGTAAGGACTCATTTGAGCTCGGTGAGGAGGCCAAACAGATGCCCTGTAAGCACTTATACCACTCGGATTGTATTCTGCCTTGGCTGGAATTGCATAACTCATGTCCTGTCTGTCGCCACGAGTTGCCCACTGATGATCCTGAATACGAGCAGAGGATCCGTGGGGACTCCGGAAATGGCAATCAAACCAGTCAATCTGTGGCTCCTGGTGGGACTAATATTTCTATGGGCGGTGGTGGGAGTGTTAGTGGGTTTGGGTTAGGAATTGGCACGCCAGCTCTAGACAATCCGAGTCCAAGTCCGCGAACGGTGGAGCGGAGGTTTACCATTTCCCTACCACGCTTTTTCGGGGCACTTGGTGGTCCGGCCGAGACTAGGGAAGGCATAACTCTGGATCGGAGGAGCCTAGGCAAGAAGATCTTGATTGAGCACAATTGCTTTTGA

Protein Analysis

376

Amino Acids

39.42

Weight (kDa)

4.93

Isoelectric Point (pI)

65.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 18 - 52 2.4e-15 zinc-ribbon
zf-RING_2 PF13639 225 - 267 4.7e-13 Ring finger domain
zf-C3HC4_2 PF13923 225 - 266 7.5e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 225 - 253 7.3e-08 RING-like zinc finger
zf-C3HC4 PF00097 226 - 266 2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 425, 590
AccBSI CCGCTC 3 cut(s) 278, 284, 1000
AccII CGCG 2 cut(s) 680, 988
AccIII TCCGGA 1 cut(s) 854
AclWI GGATC 6 cut(s) 547, 569, 815, 835, 848, 1086
AcoI YGGCCR 2 cut(s) 16, 1050
AcsI RAATTY 1 cut(s) 639
AcyI GRCGYC 2 cut(s) 216, 369
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 2 cut(s) 82, 587
AfiI CCNNNNNNNGG 2 cut(s) 464, 1059
AgsI TTSAA 1 cut(s) 376
AjnI CCWGG 2 cut(s) 457, 889
AjuI GAANNNNNNNTTGG 4 cut(s) 179, 211, 422, 454
AluBI AGCT 3 cut(s) 55, 703, 962
AluI AGCT 3 cut(s) 55, 703, 962
Alw21I GWGCWC 3 cut(s) 499, 705, 1116
Alw26I GTCTC 1 cut(s) 1049
AlwI GGATC 6 cut(s) 547, 569, 815, 835, 848, 1086
Ama87I CYCGRG 2 cut(s) 10, 535
Aor13HI TCCGGA 1 cut(s) 854
AoxI GGCC 4 cut(s) 16, 532, 714, 1050
ApeKI GCWGC 2 cut(s) 132, 453
ApoI RAATTY 1 cut(s) 639
AspA2I CCTAGG 1 cut(s) 1090
AspLEI GCGC 2 cut(s) 335, 680
AspS9I GGNCC 5 cut(s) 38, 272, 478, 533, 1046
AsuC2I CCSGG 2 cut(s) 536, 537
AsuHPI GGTGA 4 cut(s) 85, 93, 342, 719
AvaI CYCGRG 2 cut(s) 10, 535
AvaII GGWCC 4 cut(s) 38, 272, 478, 1046
AvrII CCTAGG 1 cut(s) 1090
AxyI CCTNAGG 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 1039
BamHI GGATCC 1 cut(s) 840
BanI GGYRCC 2 cut(s) 425, 590
BanII GRGCYC 1 cut(s) 705
BauI CACGAG 1 cut(s) 803
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 3 cut(s) 499, 705, 1116
BbvCI CCTCAGC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 144, 465
BccI CCATC 4 cut(s) 148, 443, 490, 623
BceAI ACGGC 1 cut(s) 604
BciT130I CCWGG 2 cut(s) 459, 891
BcnI CCSGG 2 cut(s) 536, 537
BcoDI GTCTC 1 cut(s) 1049
BfaI CTAG 3 cut(s) 965, 1059, 1091
BglI GCCNNNNNGGC 2 cut(s) 24, 462
BglII AGATCT 1 cut(s) 1101
BisI GCNGC 9 cut(s) 16, 133, 136, 139, 454, 463, 466, 469, 472
BlnI CCTAGG 1 cut(s) 1090
BlsI GCNGC 9 cut(s) 17, 134, 137, 140, 455, 464, 467, 470, 473
Bme1390I CCNGG 4 cut(s) 459, 536, 537, 891
Bme18I GGWCC 4 cut(s) 38, 272, 478, 1046
BmeT110I CYCGRG 2 cut(s) 10, 535
BmgT120I GGNCC 5 cut(s) 38, 272, 478, 533, 1046
BmiI GGNNCC 8 cut(s) 274, 427, 479, 480, 592, 842, 888, 1088
BmrFI CCNGG 4 cut(s) 459, 536, 537, 891
BmsI GCATC 2 cut(s) 549, 714
BpiI GAAGAC 1 cut(s) 379
BpmI CTGGAG 2 cut(s) 217, 377
Bpu10I CCTNAGC 1 cut(s) 51
BpuMI CCSGG 2 cut(s) 536, 537
Bsa29I ATCGAT 1 cut(s) 447
BsaHI GRCGYC 2 cut(s) 216, 369
BsaJI CCNNGG 6 cut(s) 9, 457, 535, 764, 844, 1090
BsaWI WCCGGW 1 cut(s) 854
BsaXI ACNNNNNCTCC 4 cut(s) 916, 946, 989, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 1059
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 3 cut(s) 70, 436, 873
Bse21I CCTNAGG 1 cut(s) 413
BseAI TCCGGA 1 cut(s) 854
BseBI CCWGG 2 cut(s) 459, 891
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 6 cut(s) 9, 457, 535, 764, 844, 1090
BseLI CCNNNNNNNGG 2 cut(s) 464, 1059
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 3 cut(s) 70, 436, 873
BseRI GAGGAG 9 cut(s) 199, 239, 247, 250, 307, 337, 662, 725, 1099
BseSI GKGCMC 1 cut(s) 1039
BseX3I CGGCCG 1 cut(s) 1050
BseXI GCAGC 2 cut(s) 144, 465
Bsh1236I CGCG 2 cut(s) 680, 988
Bsh1285I CGRYCG 3 cut(s) 45, 79, 1053
BshFI GGCC 4 cut(s) 18, 534, 716, 1052
BshNI GGYRCC 2 cut(s) 425, 590
BshVI ATCGAT 1 cut(s) 447
BsiEI CGRYCG 3 cut(s) 45, 79, 1053
BsiHKAI GWGCWC 3 cut(s) 499, 705, 1116
BsiHKCI CYCGRG 2 cut(s) 10, 535
BsiSI CCGG 5 cut(s) 24, 536, 629, 855, 1049
BsiWI CGTACG 1 cut(s) 80
BslFI GGGAC 4 cut(s) 258, 491, 863, 910
BslI CCNNNNNNNGG 2 cut(s) 464, 1059
BsmAI GTCTC 1 cut(s) 1049
BsmFI GGGAC 4 cut(s) 258, 491, 863, 910
BsnI GGCC 4 cut(s) 18, 534, 716, 1052
BsoBI CYCGRG 2 cut(s) 10, 535
Bsp1286I GDGCHC 4 cut(s) 499, 705, 1039, 1116
Bsp13I TCCGGA 1 cut(s) 854
BspANI GGCC 4 cut(s) 18, 534, 716, 1052
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 1 cut(s) 447
BspEI TCCGGA 1 cut(s) 854
BspFNI CGCG 2 cut(s) 680, 988
BspLI GGNNCC 8 cut(s) 274, 427, 479, 480, 592, 842, 888, 1088
BspPI GGATC 6 cut(s) 547, 569, 815, 835, 848, 1086
BspT107I GGYRCC 2 cut(s) 425, 590
BsrBI CCGCTC 3 cut(s) 278, 284, 1000
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 3 cut(s) 70, 436, 873
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 6 cut(s) 9, 457, 535, 764, 844, 1090
BssNI GRCGYC 2 cut(s) 216, 369
BssSI CACGAG 1 cut(s) 803
BssT1I CCWWGG 2 cut(s) 764, 1090
Bst2BI CACGAG 1 cut(s) 803
Bst2UI CCWGG 2 cut(s) 459, 891
Bst4CI ACNGT 4 cut(s) 80, 85, 675, 994
Bst6I CTCTTC 3 cut(s) 144, 257, 329
BstACI GRCGYC 2 cut(s) 216, 369
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 3 cut(s) 125, 956, 960
BstDEI CTNAG 2 cut(s) 51, 413
BstDSI CCRYGG 1 cut(s) 844
BstFNI CGCG 2 cut(s) 680, 988
BstHHI GCGC 2 cut(s) 335, 680
BstMAI GTCTC 1 cut(s) 1049
BstMCI CGRYCG 3 cut(s) 45, 79, 1053
BstNI CCWGG 2 cut(s) 459, 891
BstSCI CCNGG 4 cut(s) 457, 534, 535, 889
BstSLI GKGCMC 1 cut(s) 1039
BstUI CGCG 2 cut(s) 680, 988
BstV1I GCAGC 2 cut(s) 144, 465
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 840, 1101
BstYI RGATCY 2 cut(s) 840, 1101
BstZI CGGCCG 1 cut(s) 1050
Bsu15I ATCGAT 1 cut(s) 447
Bsu36I CCTNAGG 1 cut(s) 413
BsuRI GGCC 4 cut(s) 18, 534, 716, 1052
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 4 cut(s) 34, 77, 680, 813
Cac8I GCNNGC 3 cut(s) 125, 956, 960
CfoI GCGC 2 cut(s) 335, 680
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 5 cut(s) 38, 272, 478, 533, 1046
Cfr9I CCCGGG 1 cut(s) 535
ClaI ATCGAT 1 cut(s) 447
CpoI CGGWCCG 1 cut(s) 38
CseI GACGC 2 cut(s) 224, 377
Csp6I GTAC 2 cut(s) 81, 586
CspCI CAANNNNNGTGG 2 cut(s) 734, 769
CspI CGGWCCG 1 cut(s) 38
CviAII CATG 1 cut(s) 786
CviQI GTAC 2 cut(s) 81, 586
DdeI CTNAG 2 cut(s) 51, 413
DraIII CACNNNGTG 1 cut(s) 1043
EaeI YGGCCR 2 cut(s) 16, 1050
EagI CGGCCG 1 cut(s) 1050
Eam1104I CTCTTC 3 cut(s) 144, 257, 329
EarI CTCTTC 3 cut(s) 144, 257, 329
EciI GGCGGA 2 cut(s) 253, 601
Ecl136II GAGCTC 1 cut(s) 703
EclXI CGGCCG 1 cut(s) 1050
Eco130I CCWWGG 2 cut(s) 764, 1090
Eco24I GRGCYC 1 cut(s) 705
Eco47I GGWCC 4 cut(s) 38, 272, 478, 1046
Eco52I CGGCCG 1 cut(s) 1050
Eco53kI GAGCTC 1 cut(s) 703
Eco81I CCTNAGG 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 10, 535
EcoICRI GAGCTC 1 cut(s) 703
EcoO109I RGGNCCY 1 cut(s) 478
EcoRII CCWGG 2 cut(s) 457, 889
EcoT14I CCWWGG 2 cut(s) 764, 1090
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 764, 1090
FaeI CATG 1 cut(s) 789
FaiI YATR 5 cut(s) 742, 780, 787, 911, 1070
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 4 cut(s) 258, 491, 863, 910
FatI CATG 1 cut(s) 785
FauI CCCGC 6 cut(s) 132, 283, 289, 295, 467, 607
Fnu4HI GCNGC 9 cut(s) 16, 133, 136, 139, 454, 463, 466, 469, 472
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 9 cut(s) 16, 133, 136, 139, 454, 463, 466, 469, 472
FspBI CTAG 3 cut(s) 965, 1059, 1091
GlaI GCGC 2 cut(s) 334, 679
GluI GCNGC 9 cut(s) 16, 133, 136, 139, 454, 463, 466, 469, 472
GsuI CTGGAG 2 cut(s) 217, 377
HaeIII GGCC 4 cut(s) 18, 534, 716, 1052
HapII CCGG 5 cut(s) 24, 536, 629, 855, 1049
HgaI GACGC 2 cut(s) 224, 377
HhaI GCGC 2 cut(s) 335, 680
Hin1I GRCGYC 2 cut(s) 216, 369
Hin1II CATG 1 cut(s) 789
Hin6I GCGC 2 cut(s) 333, 678
HinP1I GCGC 2 cut(s) 333, 678
HinfI GANTC 4 cut(s) 665, 692, 851, 976
HpaII CCGG 5 cut(s) 24, 536, 629, 855, 1049
HphI GGTGA 4 cut(s) 85, 93, 342, 719
Hpy166II GTNNAC 1 cut(s) 1008
Hpy188I TCNGA 8 cut(s) 42, 112, 214, 646, 664, 751, 975, 1083
Hpy188III TCNNGA 8 cut(s) 359, 394, 442, 824, 855, 965, 1076, 1105
Hpy8I GTNNAC 1 cut(s) 1008
Hpy99I CGWCG 2 cut(s) 218, 371
HpyAV CCTTC 4 cut(s) 211, 235, 382, 1058
HpyCH4III ACNGT 4 cut(s) 80, 85, 675, 994
HpyCH4V TGCA 3 cut(s) 132, 406, 778
HpyF3I CTNAG 2 cut(s) 51, 413
Hsp92I GRCGYC 2 cut(s) 216, 369
Hsp92II CATG 1 cut(s) 789
HspAI GCGC 2 cut(s) 333, 678
KflI GGGWCCC 1 cut(s) 478
Kpn2I TCCGGA 1 cut(s) 854
LmnI GCTCC 7 cut(s) 283, 289, 350, 502, 892, 997, 1086
Lsp1109I GCAGC 2 cut(s) 144, 465
LweI GCATC 2 cut(s) 549, 714
MaeI CTAG 3 cut(s) 965, 1059, 1091
MbiI CCGCTC 3 cut(s) 278, 284, 1000
MboII GAAGA 6 cut(s) 161, 187, 244, 316, 379, 1111
MfeI CAATTG 2 cut(s) 548, 1117
MflI RGATCY 2 cut(s) 840, 1101
MhlI GDGCHC 4 cut(s) 499, 705, 1039, 1116
MluCI AATT 6 cut(s) 548, 560, 639, 773, 948, 1117
MlyI GAGTC 3 cut(s) 686, 845, 985
MmeI TCCRAC 2 cut(s) 237, 580
MroI TCCGGA 1 cut(s) 854
MseI TTAA 1 cut(s) 510
MslI CAYNNNNRTG 2 cut(s) 306, 673
MspA1I CMGCKG 2 cut(s) 135, 237
MspI CCGG 5 cut(s) 24, 536, 629, 855, 1049
MspR9I CCNGG 4 cut(s) 459, 536, 537, 891
MunI CAATTG 2 cut(s) 548, 1117
MvaI CCWGG 2 cut(s) 459, 891
MvnI CGCG 2 cut(s) 680, 988
NciI CCSGG 2 cut(s) 536, 537
NlaIII CATG 1 cut(s) 789
NlaIV GGNNCC 8 cut(s) 274, 427, 479, 480, 592, 842, 888, 1088
NmeAIII GCCGAG 3 cut(s) 495, 592, 1078
PfeI GAWTC 1 cut(s) 665
Pfl23II CGTACG 1 cut(s) 80
PkrI GCNGC 9 cut(s) 17, 134, 137, 140, 455, 464, 467, 470, 473
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 3 cut(s) 686, 845, 984
PpsI GAGTC 3 cut(s) 686, 845, 984
PpuMI RGGWCCY 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 2 cut(s) 457, 889
PspGI CCWGG 2 cut(s) 457, 889
PspLI CGTACG 1 cut(s) 80
PspN4I GGNNCC 8 cut(s) 274, 427, 479, 480, 592, 842, 888, 1088
PspPI GGNCC 5 cut(s) 38, 272, 478, 533, 1046
PspPPI RGGWCCY 1 cut(s) 478
PsuI RGATCY 2 cut(s) 840, 1101
PvuI CGATCG 1 cut(s) 45
RsaI GTAC 2 cut(s) 82, 587
RsaNI GTAC 2 cut(s) 81, 586
RseI CAYNNNNRTG 2 cut(s) 306, 673
Rsr2I CGGWCCG 1 cut(s) 38
RsrII CGGWCCG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 705
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 9 cut(s) 16, 133, 136, 139, 454, 463, 466, 469, 472
Sau96I GGNCC 5 cut(s) 38, 272, 478, 533, 1046
SchI GAGTC 3 cut(s) 686, 845, 985
ScrFI CCNGG 4 cut(s) 459, 536, 537, 891
SduI GDGCHC 4 cut(s) 499, 705, 1039, 1116
SetI ASST 8 cut(s) 57, 106, 110, 490, 587, 705, 964, 1007
SfaNI GCATC 2 cut(s) 549, 714
SinI GGWCC 4 cut(s) 38, 272, 478, 1046
SmaI CCCGGG 1 cut(s) 537
SmiMI CAYNNNNRTG 2 cut(s) 306, 673
SrfI GCCCGGGC 1 cut(s) 537
Sse9I AATT 6 cut(s) 548, 560, 639, 773, 948, 1117
SspI AATATT 1 cut(s) 904
SspMI CTAG 3 cut(s) 965, 1059, 1091
SstI GAGCTC 1 cut(s) 705
StyD4I CCNGG 4 cut(s) 457, 534, 535, 889
StyI CCWWGG 2 cut(s) 764, 1090
TaaI ACNGT 4 cut(s) 80, 85, 675, 994
TaqI TCGA 3 cut(s) 147, 447, 543
TasI AATT 6 cut(s) 548, 560, 639, 773, 948, 1117
TauI GCSGC 7 cut(s) 18, 138, 141, 465, 468, 471, 474
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TscAI CASTG 4 cut(s) 34, 77, 680, 820
TseI GCWGC 2 cut(s) 132, 453
TspDTI ATGAA 1 cut(s) 657
TspGWI ACGGA 1 cut(s) 833
TspMI CCCGGG 1 cut(s) 535
TspRI CASTG 4 cut(s) 34, 77, 680, 820
VpaK11BI GGWCC 4 cut(s) 38, 272, 478, 1046
XapI RAATTY 1 cut(s) 639
XbaI TCTAGA 1 cut(s) 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 880
XmaI CCCGGG 1 cut(s) 535
XmaJI CCTAGG 1 cut(s) 1090
XspI CTAG 3 cut(s) 965, 1059, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.