pycom15g00030

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010917
Physical Location & Seq
Forward (+)
25890 .. 28738
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g00030.8

Sequence Viewer

Length: 1014 bp
ATGACTATATCAGAGGATGAGGACGAGAGTTTGGCTCATTTCCTTGAATCGGAGGTGCTCTCCGAGGTTTCAGAAGACAAGGAGGAGGAGGAGGAGGATGATGAGGTAACAGAGGAGCCCAAAGCAAAGAAAATGCGACTAGTGGACGATACGGCTGAGAAAGACCCTCAATTTCAGAAAAGCGCATCTTCTTCCGGGTTTGTTCAGTCAAAAAGCGCCATCGGCTGCAGTAGCAATAGCGGGAACTCGCCCGTGAGGATTGAAACTGGCATTTTCAGCAAAATCCCTCCTGAACTCTTCCCTCACATCCTCAAGTTCCTCTCTTCTGAAGATCTATTTGCTTGCTCTCTCGTCTGTAGATTTCTAAGTCATGTCGCTTCCGATGAATCCTTATGGCGCCGCCTGTACTGTATGCGATGGGGTCATTTACCCCCTAGTAGAAAGCTGCGTGAATGTCCATGGAAAAAACTTTATATTCAGCGTGATGAGGAGGATACGGTGGAACTTGTTAGGAATTGCACACTTGAGTTCAAGGAATATTTTATTCAAATGCAAGCTGCCAAAAGAAGCCAAGCACCTCTTCCTTCACAGGTGAATGATGACATAATTCTTGACACAACAGTAGCTGATCAAGTGTCTATATGGAAAAGTAGAAGAGGCCTAACTGATAAGGCGGAAGCTGATCATGCTTGTTCAGGTGAAAAGTGCACTTACTACCAAATTGGAGATGTATTTGTCTGTGAGAACACTGGCCAGGTTCATGTATGTGATGATAATTGCAGAGAAGTTGTTATGGATCCGACCAATGAGCTTTGGGTCTGCACAATATCTGGGCACTGTTTCGATAGGCTGCTATCACCAGAAGAAACGGAGTCAGATGCTGAGCAGCAGCAAGGAGCTGTGACAGACGAGGCAGAACCATTCATGGGATCTGGGCGCTTTGCAAGAGCTTATATGATGGGATATAATTGTGTTGATGAGAAGGAGCTAGAGGATGCTTTGAGGTTTGTCTGA

Protein Analysis

338

Amino Acids

38.31

Weight (kDa)

4.55

Isoelectric Point (pI)

60.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45360
fragaria_vesca FvH4_2g37370 FvH4_2g37370
malus_domestica MD15G1000900.v1.1
prunus_persica Prupe.1G353600_v2.0.a1 Prupe.1G353600_v2.0.a1 Prupe.1G353600_v2.0.a1
pyrus_communis pycom15g00030
rosa_chinensis RchiOBHm_Chr6g0304341
rosa_laevigata RLG00000010988
rosa_multiflora Rmu_ssc0000218.1_g000040
rosa_roxburghii Rroxscaffold_7G00163820
rosa_rugosa Rorug06G0330400 Rorug06G0330500 Rorug06G0330600
rosa_samantha Rh6AG443600 Rh6BG458800 Rh6CG456400 Rh6DG443100
rosa_wichuraiana Rw6G038500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 396
AciI CCGC 3 cut(s) 240, 400, 674
AclWI GGATC 3 cut(s) 791, 804, 937
AcoI YGGCCR 1 cut(s) 751
AcuI CTGAAG 1 cut(s) 348
AcyI GRCGYC 1 cut(s) 397
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 2 cut(s) 49, 926
AgsI TTSAA 4 cut(s) 47, 263, 532, 548
AhlI ACTAGT 1 cut(s) 139
AjnI CCWGG 1 cut(s) 753
AjuI GAANNNNNNNTTGG 2 cut(s) 564, 596
AluBI AGCT 8 cut(s) 445, 557, 626, 680, 811, 899, 950, 988
AluI AGCT 8 cut(s) 445, 557, 626, 680, 811, 899, 950, 988
Alw21I GWGCWC 2 cut(s) 60, 710
Alw44I GTGCAC 1 cut(s) 706
AlwI GGATC 3 cut(s) 791, 804, 937
AlwNI CAGNNNCTG 2 cut(s) 626, 881
AoxI GGCC 2 cut(s) 658, 751
ApaLI GTGCAC 1 cut(s) 706
ApeKI GCWGC 6 cut(s) 225, 445, 557, 850, 886, 889
AspLEI GCGC 4 cut(s) 185, 218, 399, 939
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 3 cut(s) 604, 710, 849
BaeGI GKGCMC 2 cut(s) 710, 837
BalI TGGCCA 1 cut(s) 753
BamHI GGATCC 1 cut(s) 796
BanI GGYRCC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 60, 710
BbvI GCAGC 6 cut(s) 212, 432, 544, 837, 898, 901
BccI CCATC 3 cut(s) 227, 411, 952
BceAI ACGGC 1 cut(s) 168
BciT130I CCWGG 1 cut(s) 755
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 2 cut(s) 628, 682
BcnI CCSGG 1 cut(s) 196
BcuI ACTAGT 1 cut(s) 139
BfaI CTAG 3 cut(s) 140, 435, 989
BfmI CTRYAG 2 cut(s) 226, 355
BfoI RGCGCY 3 cut(s) 219, 400, 940
BfuI GTATCC 1 cut(s) 487
BglII AGATCT 1 cut(s) 331
BisI GCNGC 7 cut(s) 226, 400, 446, 558, 851, 887, 890
BlpI GCTNAGC 1 cut(s) 882
BlsI GCNGC 7 cut(s) 227, 401, 447, 559, 852, 888, 891
Bme1390I CCNGG 2 cut(s) 196, 755
BmiI GGNNCC 3 cut(s) 117, 398, 798
BmrFI CCNGG 2 cut(s) 196, 755
BmsI GCATC 3 cut(s) 194, 868, 985
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 4 cut(s) 19, 51, 44, 76
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 2 cut(s) 296, 545
BpuMI CCSGG 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 397
BsaJI CCNNGG 2 cut(s) 63, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 926
Bse1I ACTGG 2 cut(s) 271, 754
BseBI CCWGG 1 cut(s) 755
BseDI CCNNGG 2 cut(s) 63, 458
BseGI GGATG 4 cut(s) 22, 103, 306, 1000
BseLI CCNNNNNNNGG 2 cut(s) 49, 926
BseMII CTCAG 2 cut(s) 147, 873
BseNI ACTGG 2 cut(s) 271, 754
BseRI GAGGAG 6 cut(s) 98, 101, 104, 107, 128, 503
BseSI GKGCMC 2 cut(s) 710, 837
BseXI GCAGC 6 cut(s) 212, 432, 544, 837, 898, 901
BsgI GTGCAG 1 cut(s) 805
BshFI GGCC 2 cut(s) 660, 753
BshNI GGYRCC 1 cut(s) 396
BsiHKAI GWGCWC 2 cut(s) 60, 710
BsiSI CCGG 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 49, 926
BsnI GGCC 2 cut(s) 660, 753
Bsp1286I GDGCHC 4 cut(s) 60, 120, 710, 837
Bsp143I GATC 5 cut(s) 331, 628, 682, 796, 929
Bsp1720I GCTNAGC 1 cut(s) 882
Bsp19I CCATGG 1 cut(s) 458
BspACI CCGC 3 cut(s) 240, 400, 674
BspANI GGCC 2 cut(s) 660, 753
BspCNI CTCAG 2 cut(s) 148, 874
BspLI GGNNCC 3 cut(s) 117, 398, 798
BspMAI CTGCAG 1 cut(s) 230
BspPI GGATC 3 cut(s) 791, 804, 937
BspT107I GGYRCC 1 cut(s) 396
BsrI ACTGG 2 cut(s) 271, 754
BssECI CCNNGG 2 cut(s) 63, 458
BssMI GATC 5 cut(s) 331, 628, 682, 796, 929
BssNI GRCGYC 1 cut(s) 397
BssT1I CCWWGG 1 cut(s) 458
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 4 cut(s) 410, 499, 622, 839
Bst6I CTCTTC 4 cut(s) 302, 328, 585, 649
BstACI GRCGYC 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 343, 555
BstDEI CTNAG 3 cut(s) 156, 365, 882
BstDSI CCRYGG 1 cut(s) 458
BstF5I GGATG 4 cut(s) 22, 103, 306, 1000
BstH2I RGCGCY 3 cut(s) 219, 400, 940
BstHHI GCGC 4 cut(s) 185, 218, 399, 939
BstKTI GATC 5 cut(s) 334, 631, 685, 799, 932
BstMBI GATC 5 cut(s) 331, 628, 682, 796, 929
BstMWI GCNNNNNNNGC 4 cut(s) 222, 231, 276, 686
BstNI CCWGG 1 cut(s) 755
BstSCI CCNGG 2 cut(s) 194, 753
BstSFI CTRYAG 2 cut(s) 226, 355
BstSLI GKGCMC 2 cut(s) 710, 837
BstV1I GCAGC 6 cut(s) 212, 432, 544, 837, 898, 901
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 3 cut(s) 331, 796, 929
BstYI RGATCY 3 cut(s) 331, 796, 929
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 2 cut(s) 660, 753
BtgI CCRYGG 1 cut(s) 458
BtgZI GCGATG 1 cut(s) 430
BtsCI GGATG 4 cut(s) 22, 103, 306, 1000
BtsIMutI CAGTG 2 cut(s) 747, 835
Cac8I GCNNGC 2 cut(s) 343, 555
CaiI CAGNNNCTG 2 cut(s) 626, 881
CfoI GCGC 4 cut(s) 185, 218, 399, 939
Csp6I GTAC 1 cut(s) 406
CviAII CATG 5 cut(s) 371, 459, 686, 761, 925
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 3 cut(s) 156, 365, 882
DinI GGCGCC 1 cut(s) 398
DpnI GATC 5 cut(s) 333, 630, 684, 798, 931
DpnII GATC 5 cut(s) 331, 628, 682, 796, 929
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 4 cut(s) 302, 328, 585, 649
EarI CTCTTC 4 cut(s) 302, 328, 585, 649
EciI GGCGGA 1 cut(s) 689
Eco130I CCWWGG 1 cut(s) 458
Eco147I AGGCCT 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 120
EgeI GGCGCC 1 cut(s) 398
EheI GGCGCC 1 cut(s) 398
ErhI CCWWGG 1 cut(s) 458
FaeI CATG 5 cut(s) 374, 462, 689, 764, 928
FalI AAGNNNNNCTT 4 cut(s) 172, 204, 564, 596
FatI CATG 5 cut(s) 370, 458, 685, 760, 924
FauI CCCGC 1 cut(s) 233
FbaI TGATCA 2 cut(s) 628, 682
Fnu4HI GCNGC 7 cut(s) 226, 400, 446, 558, 851, 887, 890
FokI GGATG 4 cut(s) 29, 110, 293, 1007
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 7 cut(s) 226, 400, 446, 558, 851, 887, 890
FspBI CTAG 3 cut(s) 140, 435, 989
GlaI GCGC 4 cut(s) 184, 217, 398, 938
GluI GCNGC 7 cut(s) 226, 400, 446, 558, 851, 887, 890
HaeII RGCGCY 3 cut(s) 219, 400, 940
HaeIII GGCC 2 cut(s) 660, 753
HapII CCGG 1 cut(s) 195
HhaI GCGC 4 cut(s) 185, 218, 399, 939
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 5 cut(s) 374, 462, 689, 764, 928
Hin6I GCGC 4 cut(s) 183, 216, 397, 937
HinP1I GCGC 4 cut(s) 183, 216, 397, 937
HinfI GANTC 3 cut(s) 47, 386, 872
HpaII CCGG 1 cut(s) 195
HphI GGTGA 3 cut(s) 604, 710, 849
Hpy166II GTNNAC 2 cut(s) 145, 708
Hpy188III TCNNGA 2 cut(s) 290, 611
Hpy8I GTNNAC 2 cut(s) 145, 708
HpyAV CCTTC 2 cut(s) 594, 976
HpyCH4III ACNGT 4 cut(s) 410, 499, 622, 839
HpyCH4V TGCA 7 cut(s) 228, 519, 553, 708, 780, 822, 944
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 231, 276, 686
HpyF3I CTNAG 3 cut(s) 156, 365, 882
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 5 cut(s) 374, 462, 689, 764, 928
HspAI GCGC 4 cut(s) 183, 216, 397, 937
KasI GGCGCC 1 cut(s) 396
Ksp22I TGATCA 2 cut(s) 628, 682
Kzo9I GATC 5 cut(s) 331, 628, 682, 796, 929
LmnI GCTCC 3 cut(s) 115, 896, 985
Lsp1109I GCAGC 6 cut(s) 212, 432, 544, 837, 898, 901
LweI GCATC 3 cut(s) 194, 868, 985
MaeI CTAG 3 cut(s) 140, 435, 989
MaeIII GTNAC 2 cut(s) 106, 901
MalI GATC 5 cut(s) 333, 630, 684, 798, 931
MboI GATC 5 cut(s) 331, 628, 682, 796, 929
MboII GAAGA 9 cut(s) 86, 180, 183, 289, 315, 341, 572, 666, 875
MflI RGATCY 3 cut(s) 331, 796, 929
MhlI GDGCHC 4 cut(s) 60, 120, 710, 837
MlsI TGGCCA 1 cut(s) 753
MluCI AATT 6 cut(s) 170, 514, 606, 720, 775, 967
MluNI TGGCCA 1 cut(s) 753
Mly113I GGCGCC 1 cut(s) 397
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 1 cut(s) 824
Mox20I TGGCCA 1 cut(s) 753
MscI TGGCCA 1 cut(s) 753
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 1 cut(s) 753
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 196, 755
MvaI CCWGG 1 cut(s) 755
MwoI GCNNNNNNNGC 4 cut(s) 222, 231, 276, 686
NarI GGCGCC 1 cut(s) 397
NciI CCSGG 1 cut(s) 196
NcoI CCATGG 1 cut(s) 458
NdeII GATC 5 cut(s) 331, 628, 682, 796, 929
NlaIII CATG 5 cut(s) 374, 462, 689, 764, 928
NlaIV GGNNCC 3 cut(s) 117, 398, 798
NmuCI GTSAC 1 cut(s) 901
PceI AGGCCT 1 cut(s) 660
PfeI GAWTC 2 cut(s) 47, 386
PkrI GCNGC 7 cut(s) 227, 401, 447, 559, 852, 888, 891
PleI GAGTC 1 cut(s) 880
PluTI GGCGCC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 880
Psp6I CCWGG 1 cut(s) 753
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 3 cut(s) 117, 398, 798
PstI CTGCAG 1 cut(s) 230
PstNI CAGNNNCTG 2 cut(s) 626, 881
PsuI RGATCY 3 cut(s) 331, 796, 929
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 1 cut(s) 765
SatI GCNGC 7 cut(s) 226, 400, 446, 558, 851, 887, 890
Sau3AI GATC 5 cut(s) 331, 628, 682, 796, 929
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 2 cut(s) 196, 755
SduI GDGCHC 4 cut(s) 60, 120, 710, 837
SfaNI GCATC 3 cut(s) 194, 868, 985
SfcI CTRYAG 2 cut(s) 226, 355
SfoI GGCGCC 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 311, 524
SmoI CTYRAG 2 cut(s) 311, 524
SpeI ACTAGT 1 cut(s) 139
Sse9I AATT 6 cut(s) 170, 514, 606, 720, 775, 967
SseBI AGGCCT 1 cut(s) 660
SsiI CCGC 3 cut(s) 240, 400, 674
SspDI GGCGCC 1 cut(s) 396
SspI AATATT 1 cut(s) 539
SspMI CTAG 3 cut(s) 140, 435, 989
StuI AGGCCT 1 cut(s) 660
StyD4I CCNGG 2 cut(s) 194, 753
StyI CCWWGG 1 cut(s) 458
TaaI ACNGT 4 cut(s) 410, 499, 622, 839
TaqI TCGA 1 cut(s) 843
TasI AATT 6 cut(s) 170, 514, 606, 720, 775, 967
TatI WGTACW 1 cut(s) 405
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 2 cut(s) 47, 386
TscAI CASTG 2 cut(s) 754, 842
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 6 cut(s) 225, 445, 557, 850, 886, 889
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 3 cut(s) 399, 749, 913
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 2 cut(s) 754, 842
VneI GTGCAC 1 cut(s) 706
XspI CTAG 3 cut(s) 140, 435, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.