RLG00000010988

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010917
Physical Location & Seq
Reverse (-)
5846686 .. 5849700
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010988

Sequence Viewer

Length: 870 bp
ATGCATATGACCGAGGATAAGGCAGAGAATCAGAAAAGCAAAGAAGAAGATAAAGCAAGCTGCTCTTCATCTTCTTCGACTGCCATTGTCAGTAACAACCAGCTCATGAGAATTGAGACTGGGATTTTGAGCAAAATCCCACCTGAGCTGTTCCGTCACATCCTCAAGTTCCTATCTTCTGAGGATCTCATTGCTTGTTCACTCGTCTGTAGATTTCTGAATCATGCAGCTTCTGATGAATCCTTGTGGCAACGCTTGTATCGTATGCGATGGGGTCTTTTGCCCCCTACCAGAAAGCTACGCGAATGTCCTTGGAAAAAGCTTTACATTGAGCGTGATGAAGAGGACATGGTGATTCTTGGTAGAAATTGCCCGGTTGAGTTCAAGGAGTACTATGTCCAAATGCAAGCTGCCAAAAGAAGCCAAGCACCTCTTCCTTCACAGGCGAAAGATGATGGGTTAATTCTTGACAAGACAGTAGCTGATCAAGTGTCCATCTGGAAAAGAAGTAAAGGCCTGACTGATAAGGTGGTAACTGATCATACTTGTTCCGGTGAAAAGTGTACTTACTATCAAGTTGGAGATGTGTTTGTTTGCGAGAAGACTGGGCAGGTTCATGTATGTGATGAGTTTTGCAGAGAAGCTGTTATGGATCCCGCTAATATGCTTTGGGTCTGTAGAATATCTGGTCATTGCTTTGATAATGTACTGTCACCACAAGAAGTGGAGTCAGATGCAGAACAGCAACAGGAAGATCCGACAGATGAGCCAGAACCATTCATGGGATCCGGCCGCTTTGCGCGAGCTTATTTGCTGGGATATAACTGTGCGGATGAGAAAGAGCTAGAAGCTACTTGGAGGTTTTTGTGA

Protein Analysis

290

Amino Acids

33.14

Weight (kDa)

5.16

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 84 4.2e-09 F-box domain
F-box-like PF12937 44 - 88 9.7e-14 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45360
fragaria_vesca FvH4_2g37370 FvH4_2g37370
malus_domestica MD15G1000900.v1.1
prunus_persica Prupe.1G353600_v2.0.a1 Prupe.1G353600_v2.0.a1 Prupe.1G353600_v2.0.a1
pyrus_communis pycom15g00030
rosa_chinensis RchiOBHm_Chr6g0304341
rosa_laevigata RLG00000010988
rosa_multiflora Rmu_ssc0000218.1_g000040
rosa_roxburghii Rroxscaffold_7G00163820
rosa_rugosa Rorug06G0330400 Rorug06G0330500 Rorug06G0330600
rosa_samantha Rh6AG443600 Rh6BG458800 Rh6CG456400 Rh6DG443100
rosa_wichuraiana Rw6G038500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 601
AccII CGCG 2 cut(s) 303, 802
AciI CCGC 3 cut(s) 657, 793, 830
AclWI GGATC 6 cut(s) 192, 647, 660, 749, 780, 793
AcoI YGGCCR 1 cut(s) 790
AfaI GTAC 3 cut(s) 392, 565, 708
AfiI CCNNNNNNNGG 1 cut(s) 782
AgsI TTSAA 1 cut(s) 385
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 6 cut(s) 192, 647, 660, 749, 780, 793
AlwNI CAGNNNCTG 2 cut(s) 233, 482
AoxI GGCC 2 cut(s) 514, 790
ApeKI GCWGC 3 cut(s) 60, 227, 410
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
AspLEI GCGC 1 cut(s) 802
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 3 cut(s) 364, 566, 705
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BamHI GGATCC 2 cut(s) 652, 785
BbsI GAAGAC 1 cut(s) 608
BbvI GCAGC 3 cut(s) 47, 239, 397
BccI CCATC 3 cut(s) 264, 449, 503
BclI TGATCA 2 cut(s) 484, 538
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 845
BfmI CTRYAG 2 cut(s) 208, 676
BfuAI ACCTGC 1 cut(s) 601
BisI GCNGC 4 cut(s) 61, 228, 411, 793
BlsI GCNGC 4 cut(s) 62, 229, 412, 794
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 654, 787
BmrFI CCNGG 1 cut(s) 374
BmrI ACTGGG 2 cut(s) 129, 615
BmsI GCATC 1 cut(s) 724
BmuI ACTGGG 2 cut(s) 129, 615
BpiI GAAGAC 1 cut(s) 608
Bpu10I CCTNAGC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 12, 311
BsaWI WCCGGW 1 cut(s) 551
Bsc4I CCNNNNNNNGG 1 cut(s) 782
Bse1I ACTGG 2 cut(s) 124, 610
Bse3DI GCAATG 2 cut(s) 189, 691
BseDI CCNNGG 2 cut(s) 12, 311
BseGI GGATG 2 cut(s) 159, 838
BseLI CCNNNNNNNGG 1 cut(s) 782
BseMI GCAATG 2 cut(s) 189, 691
BseMII CTCAG 2 cut(s) 135, 171
BseNI ACTGG 2 cut(s) 124, 610
BseX3I CGGCCG 1 cut(s) 790
BseXI GCAGC 3 cut(s) 47, 239, 397
BseYI CCCAGC 1 cut(s) 814
Bsh1236I CGCG 2 cut(s) 303, 802
Bsh1285I CGRYCG 1 cut(s) 793
BshFI GGCC 2 cut(s) 516, 792
BsiEI CGRYCG 1 cut(s) 793
BsiSI CCGG 3 cut(s) 374, 552, 789
BslI CCNNNNNNNGG 1 cut(s) 782
BsmAI GTCTC 1 cut(s) 110
BsnI GGCC 2 cut(s) 516, 792
Bsp143I GATC 6 cut(s) 184, 484, 538, 652, 754, 785
BspACI CCGC 3 cut(s) 657, 793, 830
BspANI GGCC 2 cut(s) 516, 792
BspCNI CTCAG 2 cut(s) 136, 172
BspFNI CGCG 2 cut(s) 303, 802
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 654, 787
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 6 cut(s) 192, 647, 660, 749, 780, 793
BspQI GCTCTTC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 189, 691
BsrI ACTGG 2 cut(s) 124, 610
BssECI CCNNGG 2 cut(s) 12, 311
BssMI GATC 6 cut(s) 184, 484, 538, 652, 754, 785
BssT1I CCWWGG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 478, 711, 827
Bst6I CTCTTC 3 cut(s) 70, 336, 438
BstC8I GCNNGC 3 cut(s) 58, 408, 804
BstDEI CTNAG 2 cut(s) 144, 180
BstF5I GGATG 2 cut(s) 159, 838
BstFNI CGCG 2 cut(s) 303, 802
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 6 cut(s) 187, 487, 541, 655, 757, 788
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 6 cut(s) 184, 484, 538, 652, 754, 785
BstMCI CGRYCG 1 cut(s) 793
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 2 cut(s) 208, 676
BstUI CGCG 2 cut(s) 303, 802
BstV1I GCAGC 3 cut(s) 47, 239, 397
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 4 cut(s) 184, 652, 754, 785
BstYI RGATCY 4 cut(s) 184, 652, 754, 785
BstZI CGGCCG 1 cut(s) 790
BsuRI GGCC 2 cut(s) 516, 792
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 159, 838
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 3 cut(s) 58, 408, 804
CaiI CAGNNNCTG 2 cut(s) 233, 482
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 802
Csp6I GTAC 3 cut(s) 391, 564, 707
CviAII CATG 5 cut(s) 106, 224, 349, 617, 781
CviQI GTAC 3 cut(s) 391, 564, 707
DdeI CTNAG 2 cut(s) 144, 180
DpnI GATC 6 cut(s) 186, 486, 540, 654, 756, 787
DpnII GATC 6 cut(s) 184, 484, 538, 652, 754, 785
EaeI YGGCCR 1 cut(s) 790
EagI CGGCCG 1 cut(s) 790
Eam1104I CTCTTC 3 cut(s) 70, 336, 438
EarI CTCTTC 3 cut(s) 70, 336, 438
EclXI CGGCCG 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 311
Eco147I AGGCCT 1 cut(s) 516
Eco52I CGGCCG 1 cut(s) 790
EcoT14I CCWWGG 1 cut(s) 311
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 5 cut(s) 109, 227, 352, 620, 784
FalI AAGNNNNNCTT 4 cut(s) 49, 81, 417, 449
FatI CATG 5 cut(s) 105, 223, 348, 616, 780
FauI CCCGC 1 cut(s) 664
FauNDI CATATG 1 cut(s) 6
FbaI TGATCA 2 cut(s) 484, 538
Fnu4HI GCNGC 4 cut(s) 61, 228, 411, 793
FokI GGATG 2 cut(s) 146, 845
Fsp4HI GCNGC 4 cut(s) 61, 228, 411, 793
FspBI CTAG 1 cut(s) 845
GlaI GCGC 1 cut(s) 801
GluI GCNGC 4 cut(s) 61, 228, 411, 793
GsaI CCCAGC 1 cut(s) 818
HaeIII GGCC 2 cut(s) 516, 792
HapII CCGG 3 cut(s) 374, 552, 789
HhaI GCGC 1 cut(s) 802
Hin1II CATG 5 cut(s) 109, 227, 352, 620, 784
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 5 cut(s) 28, 220, 239, 355, 728
HpaII CCGG 3 cut(s) 374, 552, 789
HphI GGTGA 3 cut(s) 364, 566, 705
Hpy166II GTNNAC 2 cut(s) 200, 564
Hpy188I TCNGA 6 cut(s) 33, 181, 219, 235, 733, 759
Hpy188III TCNNGA 3 cut(s) 106, 467, 499
Hpy8I GTNNAC 2 cut(s) 200, 564
HpyAV CCTTC 1 cut(s) 447
HpyCH4III ACNGT 3 cut(s) 478, 711, 827
HpyCH4V TGCA 5 cut(s) 4, 227, 406, 636, 737
HpyF3I CTNAG 2 cut(s) 144, 180
Hsp92II CATG 5 cut(s) 109, 227, 352, 620, 784
HspAI GCGC 1 cut(s) 800
Ksp22I TGATCA 2 cut(s) 484, 538
Kzo9I GATC 6 cut(s) 184, 484, 538, 652, 754, 785
LguI GCTCTTC 1 cut(s) 70
Lsp1109I GCAGC 3 cut(s) 47, 239, 397
LweI GCATC 1 cut(s) 724
MaeI CTAG 1 cut(s) 845
MaeIII GTNAC 4 cut(s) 92, 155, 532, 711
MalI GATC 6 cut(s) 186, 486, 540, 654, 756, 787
MboI GATC 6 cut(s) 184, 484, 538, 652, 754, 785
MflI RGATCY 4 cut(s) 184, 652, 754, 785
MluCI AATT 3 cut(s) 111, 367, 462
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 2 cut(s) 559, 782
MnlI CCTC 6 cut(s) 7, 173, 175, 337, 441, 852
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 461
MslI CAYNNNNRTG 1 cut(s) 621
MspI CCGG 3 cut(s) 374, 552, 789
MspR9I CCNGG 1 cut(s) 374
MvnI CGCG 2 cut(s) 303, 802
NciI CCSGG 1 cut(s) 374
NdeI CATATG 1 cut(s) 6
NdeII GATC 6 cut(s) 184, 484, 538, 652, 754, 785
NlaIII CATG 5 cut(s) 109, 227, 352, 620, 784
NlaIV GGNNCC 2 cut(s) 654, 787
NmuCI GTSAC 2 cut(s) 155, 711
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 105
PceI AGGCCT 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 4 cut(s) 28, 220, 239, 355
PkrI GCNGC 4 cut(s) 62, 229, 412, 794
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PspFI CCCAGC 1 cut(s) 814
PspN4I GGNNCC 2 cut(s) 654, 787
PstNI CAGNNNCTG 2 cut(s) 233, 482
PsuI RGATCY 4 cut(s) 184, 652, 754, 785
RsaI GTAC 3 cut(s) 392, 565, 708
RsaNI GTAC 3 cut(s) 391, 564, 707
RseI CAYNNNNRTG 1 cut(s) 621
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 4 cut(s) 61, 228, 411, 793
Sau3AI GATC 6 cut(s) 184, 484, 538, 652, 754, 785
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 1 cut(s) 374
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 2 cut(s) 208, 676
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 111, 367, 462
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 3 cut(s) 657, 793, 830
SspMI CTAG 1 cut(s) 845
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 3 cut(s) 478, 711, 827
TaqI TCGA 1 cut(s) 77
TaqII GACCGA 1 cut(s) 26
TasI AATT 3 cut(s) 111, 367, 462
TatI WGTACW 3 cut(s) 390, 563, 706
TauI GCSGC 1 cut(s) 795
TfiI GAWTC 4 cut(s) 28, 220, 239, 355
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TseFI GTSAC 2 cut(s) 155, 711
TseI GCWGC 3 cut(s) 60, 227, 410
Tsp45I GTSAC 2 cut(s) 155, 711
TspDTI ATGAA 5 cut(s) 57, 252, 354, 605, 769
TspGWI ACGGA 1 cut(s) 143
XspI CTAG 1 cut(s) 845
ZrmI AGTACT 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.