Rroxscaffold_7G00163820

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010917
Physical Location & Seq
Reverse (-)
5991883 .. 5995683
3801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163820.1

Sequence Viewer

Length: 1194 bp
ATGACGATATCAGAGGACGAGGACGAGAGTTTGGCTCAATTCCTTGAAACGGAGGTGCTCTCCGAGGTCTCCGATCAGGAGGAGGAAGATTTGATGATAAAGGAGCCCAACCCTAAGAGAATGCATATGACCGAGGATAAGGCAGAGAATCAGATAAGCAAAGAAGAAGAAGATAAAGCAAGCTGCTCTTCATCTTCTTCGACCGCCATTGTCAGTAACAACCAGCTCATGAGAATTGAGACTGGGATTTTGAGCAAAATCCCACCTGAGCTGTTCCGTCACATCCTCAAGTTCCTATCTTCTGAGGATCTCATAGCTTGTTCACTCGTCTGTAGATTTCTGAATCATGCAGCTTCTGATGAATCCTTGTGGCAACGCTTGTATCGTATGCGATGGGGTCTTTTGCCCCCTACCAGAAAGTTGCGGGAATGTCCTTGGAAAAAGCTTTACATTGAGCGTGATGAAGAGGACATGGTGGATCTTGGTAGAAATTGCCCGGTTGAGTTCAAGGAGTACTATGTCCAAATGCAAGCTGCCAAAAGAAGCCAAGCACCTCTTCCTTCACAGAGAGGTGCCGACAAGAGGGCTAATACTCTGTGGAACTGTGCTGTTTTGGCCTCTTTGTGGGTGGTATGGTTGGAAAGATATAGAGCAATTATTGAAGACAAGGAAGTGTCTTTAGTACGTGACAGAATCAAGTATATGTCTCGTTGGGCTTCTATGTCTAGGCATTTTATTCGTGTTCCTTGTGGGTTAAGGGTTTTGCAGGCGAAAGATGATGGGTTAATTCTTGACAAGACAGTAGCTGATCAAGTGTCCATCTGGAAAAGAAGTAAAGGCCTGACTGATAAGGTGGTAACTGATCATACTTGTTCTGGTGAAAAGTGTACTTACTACCAAGTTGGAGATGTGTTTGTTTGCGAGAAGACTGGGCAGGTTCATGTATGCGATGAGTTTTGCAGAGAAGCTGTTATGGATCCCGCTAATATGCTTTGGGTCTGTAGAATATCTGGTCATTGCTTTGATAATGTACTGTCACCACAAGAAGTGGAGTCAGATGCAGAACAGCAACAGGAAGATCCGACAGATGAGCCAGAACCATTCATGGGATCCGGCCGCTTTGCGCGAGCTTATTTGCTGGGATATAACTGTGCGGATGAGAAAGAGCTAGAAGCTACTTGGAGGTTTTTGTGA

Protein Analysis

397

Amino Acids

45.87

Weight (kDa)

5.01

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 84 - 125 6.7e-09 F-box domain
F-box-like PF12937 85 - 129 1.5e-13 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45360
fragaria_vesca FvH4_2g37370 FvH4_2g37370
malus_domestica MD15G1000900.v1.1
prunus_persica Prupe.1G353600_v2.0.a1 Prupe.1G353600_v2.0.a1 Prupe.1G353600_v2.0.a1
pyrus_communis pycom15g00030
rosa_chinensis RchiOBHm_Chr6g0304341
rosa_laevigata RLG00000010988
rosa_multiflora Rmu_ssc0000218.1_g000040
rosa_roxburghii Rroxscaffold_7G00163820
rosa_rugosa Rorug06G0330400 Rorug06G0330500 Rorug06G0330600
rosa_samantha Rh6AG443600 Rh6BG458800 Rh6CG456400 Rh6DG443100
rosa_wichuraiana Rw6G038500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 925
AccB1I GGYRCC 1 cut(s) 572
AccII CGCG 1 cut(s) 1126
AciI CCGC 5 cut(s) 204, 424, 981, 1117, 1154
AclWI GGATC 7 cut(s) 315, 486, 971, 984, 1073, 1104, 1117
AcoI YGGCCR 1 cut(s) 1114
AfaI GTAC 4 cut(s) 515, 684, 889, 1032
AfiI CCNNNNNNNGG 4 cut(s) 49, 582, 624, 1106
AgsI TTSAA 3 cut(s) 47, 508, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 3 cut(s) 73, 233, 711
AlwI GGATC 7 cut(s) 315, 486, 971, 984, 1073, 1104, 1117
AlwNI CAGNNNCTG 2 cut(s) 356, 806
AoxI GGCC 3 cut(s) 615, 838, 1114
ApeKI GCWGC 3 cut(s) 183, 350, 533
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AspLEI GCGC 1 cut(s) 1126
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 2 cut(s) 890, 1029
BaeI ACNNNNGTAYC 2 cut(s) 365, 398
BamHI GGATCC 2 cut(s) 976, 1109
BanI GGYRCC 1 cut(s) 572
BanII GRGCYC 1 cut(s) 108
BbsI GAAGAC 2 cut(s) 669, 932
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 170, 362, 520
BccI CCATC 3 cut(s) 387, 773, 827
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BclI TGATCA 2 cut(s) 808, 862
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 3 cut(s) 73, 233, 711
BfaI CTAG 2 cut(s) 726, 1169
BfmI CTRYAG 2 cut(s) 331, 1000
BfuAI ACCTGC 1 cut(s) 925
BisI GCNGC 4 cut(s) 184, 351, 534, 1117
BlsI GCNGC 4 cut(s) 185, 352, 535, 1118
BmcAI AGTACT 1 cut(s) 515
Bme1390I CCNGG 1 cut(s) 497
BmiI GGNNCC 4 cut(s) 105, 574, 978, 1111
BmrFI CCNGG 1 cut(s) 497
BmrI ACTGGG 2 cut(s) 252, 939
BmsI GCATC 1 cut(s) 1048
BmuI ACTGGG 2 cut(s) 252, 939
BpiI GAAGAC 2 cut(s) 669, 932
BplI GAGNNNNNCTC 4 cut(s) 19, 51, 44, 76
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 686
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 3 cut(s) 63, 132, 434
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 582, 624, 1106
Bse1I ACTGG 2 cut(s) 247, 934
Bse3DI GCAATG 1 cut(s) 1015
BseDI CCNNGG 3 cut(s) 63, 132, 434
BseGI GGATG 2 cut(s) 282, 1162
BseLI CCNNNNNNNGG 4 cut(s) 49, 582, 624, 1106
BseMI GCAATG 1 cut(s) 1015
BseMII CTCAG 2 cut(s) 258, 294
BseNI ACTGG 2 cut(s) 247, 934
BseRI GAGGAG 1 cut(s) 95
BseX3I CGGCCG 1 cut(s) 1114
BseXI GCAGC 3 cut(s) 170, 362, 520
BseYI CCCAGC 1 cut(s) 1138
Bsh1236I CGCG 1 cut(s) 1126
Bsh1285I CGRYCG 2 cut(s) 204, 1117
BshFI GGCC 3 cut(s) 617, 840, 1116
BshNI GGYRCC 1 cut(s) 572
BsiEI CGRYCG 2 cut(s) 204, 1117
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 2 cut(s) 497, 1113
BslI CCNNNNNNNGG 4 cut(s) 49, 582, 624, 1106
BsmAI GTCTC 3 cut(s) 73, 233, 711
BsmI GAATGC 1 cut(s) 126
BsnI GGCC 3 cut(s) 617, 840, 1116
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 60, 108
Bsp143I GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
BspACI CCGC 5 cut(s) 204, 424, 981, 1117, 1154
BspANI GGCC 3 cut(s) 617, 840, 1116
BspCNI CTCAG 2 cut(s) 259, 295
BspFNI CGCG 1 cut(s) 1126
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 4 cut(s) 105, 574, 978, 1111
BspMI ACCTGC 1 cut(s) 925
BspPI GGATC 7 cut(s) 315, 486, 971, 984, 1073, 1104, 1117
BspQI GCTCTTC 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 572
BspTNI GGTCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 1015
BsrI ACTGG 2 cut(s) 247, 934
BssECI CCNNGG 3 cut(s) 63, 132, 434
BssMI GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
BssT1I CCWWGG 1 cut(s) 434
Bst4CI ACNGT 4 cut(s) 605, 802, 1035, 1151
Bst6I CTCTTC 3 cut(s) 193, 459, 561
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 4 cut(s) 181, 531, 768, 1128
BstDEI CTNAG 3 cut(s) 114, 267, 303
BstF5I GGATG 2 cut(s) 282, 1162
BstFNI CGCG 1 cut(s) 1126
BstHHI GCGC 1 cut(s) 1126
BstKTI GATC 8 cut(s) 76, 310, 481, 811, 865, 979, 1081, 1112
BstMAI GTCTC 3 cut(s) 73, 233, 711
BstMBI GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
BstMCI CGRYCG 2 cut(s) 204, 1117
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 495
BstSFI CTRYAG 2 cut(s) 331, 1000
BstUI CGCG 1 cut(s) 1126
BstV1I GCAGC 3 cut(s) 170, 362, 520
BstV2I GAAGAC 2 cut(s) 669, 932
BstX2I RGATCY 5 cut(s) 307, 478, 976, 1078, 1109
BstYI RGATCY 5 cut(s) 307, 478, 976, 1078, 1109
BstZI CGGCCG 1 cut(s) 1114
BsuRI GGCC 3 cut(s) 617, 840, 1116
BtgZI GCGATG 2 cut(s) 406, 963
BtsCI GGATG 2 cut(s) 282, 1162
BveI ACCTGC 1 cut(s) 925
Cac8I GCNNGC 4 cut(s) 181, 531, 768, 1128
CaiI CAGNNNCTG 2 cut(s) 356, 806
CciI TCATGA 1 cut(s) 228
CfoI GCGC 1 cut(s) 1126
Csp6I GTAC 4 cut(s) 514, 683, 888, 1031
CviAII CATG 5 cut(s) 229, 347, 472, 941, 1105
CviQI GTAC 4 cut(s) 514, 683, 888, 1031
DdeI CTNAG 3 cut(s) 114, 267, 303
DpnI GATC 8 cut(s) 75, 309, 480, 810, 864, 978, 1080, 1111
DpnII GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
EaeI YGGCCR 1 cut(s) 1114
EagI CGGCCG 1 cut(s) 1114
Eam1104I CTCTTC 3 cut(s) 193, 459, 561
EarI CTCTTC 3 cut(s) 193, 459, 561
EclXI CGGCCG 1 cut(s) 1114
Eco130I CCWWGG 1 cut(s) 434
Eco147I AGGCCT 1 cut(s) 840
Eco24I GRGCYC 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 73
Eco32I GATATC 1 cut(s) 9
Eco52I CGGCCG 1 cut(s) 1114
EcoRV GATATC 1 cut(s) 9
EcoT14I CCWWGG 1 cut(s) 434
EcoT22I ATGCAT 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 434
FaeI CATG 5 cut(s) 232, 350, 475, 944, 1108
FalI AAGNNNNNCTT 4 cut(s) 172, 204, 540, 572
FatI CATG 5 cut(s) 228, 346, 471, 940, 1104
FauI CCCGC 2 cut(s) 417, 988
FauNDI CATATG 1 cut(s) 126
FbaI TGATCA 2 cut(s) 808, 862
Fnu4HI GCNGC 4 cut(s) 184, 351, 534, 1117
FokI GGATG 2 cut(s) 269, 1169
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 4 cut(s) 184, 351, 534, 1117
FspBI CTAG 2 cut(s) 726, 1169
GlaI GCGC 1 cut(s) 1125
GluI GCNGC 4 cut(s) 184, 351, 534, 1117
GsaI CCCAGC 1 cut(s) 1142
HaeIII GGCC 3 cut(s) 617, 840, 1116
HapII CCGG 2 cut(s) 497, 1113
HhaI GCGC 1 cut(s) 1126
Hin1II CATG 5 cut(s) 232, 350, 475, 944, 1108
Hin6I GCGC 1 cut(s) 1124
HinP1I GCGC 1 cut(s) 1124
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 5 cut(s) 148, 343, 362, 693, 1052
HpaII CCGG 2 cut(s) 497, 1113
HphI GGTGA 2 cut(s) 890, 1029
Hpy166II GTNNAC 2 cut(s) 323, 888
Hpy188I TCNGA 9 cut(s) 13, 64, 73, 153, 304, 342, 358, 1057, 1083
Hpy188III TCNNGA 4 cut(s) 77, 229, 791, 823
Hpy8I GTNNAC 2 cut(s) 323, 888
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 4 cut(s) 605, 802, 1035, 1151
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 6 cut(s) 124, 350, 529, 766, 960, 1061
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 3 cut(s) 114, 267, 303
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 5 cut(s) 232, 350, 475, 944, 1108
HspAI GCGC 1 cut(s) 1124
Ksp22I TGATCA 2 cut(s) 808, 862
Kzo9I GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
LguI GCTCTTC 1 cut(s) 193
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 3 cut(s) 170, 362, 520
LweI GCATC 1 cut(s) 1048
MaeI CTAG 2 cut(s) 726, 1169
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 5 cut(s) 215, 278, 686, 856, 1035
MalI GATC 8 cut(s) 75, 309, 480, 810, 864, 978, 1080, 1111
MboI GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
MflI RGATCY 5 cut(s) 307, 478, 976, 1078, 1109
MhlI GDGCHC 2 cut(s) 60, 108
MluCI AATT 5 cut(s) 38, 234, 490, 654, 786
MlyI GAGTC 1 cut(s) 1061
MmeI TCCRAC 3 cut(s) 618, 883, 1106
Mph1103I ATGCAT 1 cut(s) 126
MseI TTAA 2 cut(s) 755, 785
MspI CCGG 2 cut(s) 497, 1113
MspR9I CCNGG 1 cut(s) 497
Mva1269I GAATGC 1 cut(s) 126
MvnI CGCG 1 cut(s) 1126
MwoI GCNNNNNNNGC 1 cut(s) 614
NciI CCSGG 1 cut(s) 497
NdeI CATATG 1 cut(s) 126
NdeII GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
NlaIII CATG 5 cut(s) 232, 350, 475, 944, 1108
NlaIV GGNNCC 4 cut(s) 105, 574, 978, 1111
NmuCI GTSAC 3 cut(s) 278, 686, 1035
NsiI ATGCAT 1 cut(s) 126
PagI TCATGA 1 cut(s) 228
PceI AGGCCT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PctI GAATGC 1 cut(s) 126
PfeI GAWTC 4 cut(s) 148, 343, 362, 693
PkrI GCNGC 4 cut(s) 185, 352, 535, 1118
PleI GAGTC 1 cut(s) 1060
PpsI GAGTC 1 cut(s) 1060
Ppu21I YACGTR 1 cut(s) 686
PspFI CCCAGC 1 cut(s) 1138
PspN4I GGNNCC 4 cut(s) 105, 574, 978, 1111
PstNI CAGNNNCTG 2 cut(s) 356, 806
PsuI RGATCY 5 cut(s) 307, 478, 976, 1078, 1109
RsaI GTAC 4 cut(s) 515, 684, 889, 1032
RsaNI GTAC 4 cut(s) 514, 683, 888, 1031
SapI GCTCTTC 1 cut(s) 193
SaqAI TTAA 2 cut(s) 755, 785
SatI GCNGC 4 cut(s) 184, 351, 534, 1117
Sau3AI GATC 8 cut(s) 73, 307, 478, 808, 862, 976, 1078, 1109
ScaI AGTACT 1 cut(s) 515
SchI GAGTC 1 cut(s) 1061
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 2 cut(s) 60, 108
SfaNI GCATC 1 cut(s) 1048
SfcI CTRYAG 2 cut(s) 331, 1000
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 5 cut(s) 38, 234, 490, 654, 786
SseBI AGGCCT 1 cut(s) 840
SsiI CCGC 5 cut(s) 204, 424, 981, 1117, 1154
SspMI CTAG 2 cut(s) 726, 1169
StuI AGGCCT 1 cut(s) 840
StyD4I CCNGG 1 cut(s) 495
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 4 cut(s) 605, 802, 1035, 1151
TaiI ACGT 1 cut(s) 688
TaqI TCGA 1 cut(s) 200
TaqII GACCGA 1 cut(s) 146
TasI AATT 5 cut(s) 38, 234, 490, 654, 786
TatI WGTACW 3 cut(s) 513, 887, 1030
TauI GCSGC 1 cut(s) 1119
TfiI GAWTC 4 cut(s) 148, 343, 362, 693
Tru1I TTAA 2 cut(s) 755, 785
Tru9I TTAA 2 cut(s) 755, 785
TseFI GTSAC 3 cut(s) 278, 686, 1035
TseI GCWGC 3 cut(s) 183, 350, 533
Tsp45I GTSAC 3 cut(s) 278, 686, 1035
TspDTI ATGAA 5 cut(s) 180, 375, 477, 929, 1093
TspGWI ACGGA 2 cut(s) 65, 266
XspI CTAG 2 cut(s) 726, 1169
ZrmI AGTACT 1 cut(s) 515
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.